AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63354

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63354.t1 MTR_3g106420 90.833 480 15 2 1 451 1 480 0.0 875
MS.gene63354.t1 MTR_3g106480 87.866 478 29 2 3 451 1 478 0.0 841
MS.gene63354.t1 MTR_3g106485 89.451 474 25 1 3 451 1 474 0.0 827
MS.gene63354.t1 MTR_1g083190 88.655 476 25 2 1 451 1 472 0.0 816
MS.gene63354.t1 MTR_1g073400 87.764 474 33 1 3 451 1 474 0.0 795
MS.gene63354.t1 MTR_1g071130 85.714 455 53 3 3 451 1 449 0.0 786
MS.gene63354.t1 MTR_1g115145 85.084 476 41 4 3 451 1 473 0.0 785
MS.gene63354.t1 MTR_3g106430 86.892 473 37 1 3 450 1 473 0.0 771
MS.gene63354.t1 MTR_3g106470 84.176 455 31 4 3 450 32 452 0.0 723
MS.gene63354.t1 MTR_8g063830 75.862 464 44 6 3 437 1 425 0.0 615
MS.gene63354.t1 MTR_3g065540 64.348 115 14 1 42 156 14 101 3.08e-40 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63354.t1 AT5G25260 70.464 474 99 4 3 447 1 462 0.0 676
MS.gene63354.t1 AT5G25250 70.426 470 102 3 3 446 1 459 0.0 671
MS.gene63354.t1 AT5G64870 67.161 472 115 4 4 446 3 463 0.0 607

Find 113 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGAGTATTTCTCATATT+TGG 0.110239 3.3:+59658588 MS.gene63354:CDS
TTGAACCTAAGATTAGTATT+TGG 0.251111 3.3:+59659805 MS.gene63354:CDS
CATGAGTATTTCTCATATTT+GGG 0.292202 3.3:+59658589 MS.gene63354:CDS
AGGGATTACTTGATGATAAA+TGG 0.297566 3.3:+59659732 MS.gene63354:CDS
CGAAGAAGGCTGTGGCTCTA+AGG 0.317541 3.3:+59658917 MS.gene63354:CDS
GTCAACCCTAGCTTGATTCT+TGG 0.318930 3.3:-59658632 None:intergenic
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 3.3:+59658664 MS.gene63354:CDS
AACAAGTTCATTAACGTGAT+TGG 0.321473 3.3:-59658389 None:intergenic
TTAGTCCAAATACTAATCTT+AGG 0.325227 3.3:-59659810 None:intergenic
TATCATCAAGTAATCCCTTA+TGG 0.339784 3.3:-59659727 None:intergenic
CAACTTGAGTTGAATCAATT+CGG 0.350972 3.3:+59658517 MS.gene63354:CDS
ACTTCGAAGGTGTAGTTTAC+CGG 0.380695 3.3:-59658241 None:intergenic
GTGAAATTGGTTCAAAATTG+AGG 0.384625 3.3:+59658677 MS.gene63354:CDS
CAATGCGAATGTTAAGCAAT+TGG 0.388576 3.3:+59658552 MS.gene63354:CDS
AAGATTAGTATTTGGACTAA+TGG 0.394006 3.3:+59659813 MS.gene63354:CDS
TCAGAATACCTTGTGATAAC+AGG 0.405430 3.3:+59658139 MS.gene63354:CDS
TGTAGGTACAAGAAGCAAAC+TGG 0.406248 3.3:+59659478 MS.gene63354:intron
TGGTCCTCGTGTTGACGATC+AGG 0.407219 3.3:+59658318 MS.gene63354:CDS
GGCTTGGACCAAGGCGGCTC+AGG 0.413477 3.3:+59658879 MS.gene63354:CDS
CACGTTAATGAACTTGTTCA+AGG 0.425303 3.3:+59658394 MS.gene63354:CDS
TTCAGCCACCTGAGCCGCCT+TGG 0.429757 3.3:-59658887 None:intergenic
CATGTTGCCTCCTGCATGGA+TGG 0.431761 3.3:+59659932 MS.gene63354:CDS
TGAAATTGGTTCAAAATTGA+GGG 0.433449 3.3:+59658678 MS.gene63354:CDS
CTCCCCGCTGTCTTCACCAT+TGG 0.433479 3.3:+59658298 MS.gene63354:CDS
ATCATGTGGTGAAATAAGTT+TGG 0.438830 3.3:-59658359 None:intergenic
CTTTGCATATAGCTCTGCTT+CGG 0.455229 3.3:-59659613 None:intergenic
CGGTCAATCCTGCACCGTCT+TGG 0.457748 3.3:+59658210 MS.gene63354:CDS
GCAGAGGGAACCGTGACCCT+TGG 0.465294 3.3:+59659645 MS.gene63354:CDS
GGCTTAAGCTAATTCTGAGT+TGG 0.466949 3.3:+59658843 MS.gene63354:intron
CAAGACGGTGCAGGATTGAC+CGG 0.470050 3.3:-59658209 None:intergenic
GGCTGAAGTTGAGGCGAAGA+AGG 0.470534 3.3:+59658903 MS.gene63354:CDS
CAGAGGGAACCGTGACCCTT+GGG 0.472084 3.3:+59659646 MS.gene63354:CDS
ATTAGTATTTGGACTAATGG+TGG 0.472603 3.3:+59659816 MS.gene63354:CDS
TTGGACCAAGGCGGCTCAGG+TGG 0.476684 3.3:+59658882 MS.gene63354:CDS
CATCAAGAATGAAGATGTAC+CGG 0.478871 3.3:+59658104 None:intergenic
GAAACAACTACACAGCCATA+AGG 0.486130 3.3:+59659712 MS.gene63354:CDS
GGACCAATGGTGAAGACAGC+GGG 0.486586 3.3:-59658301 None:intergenic
GGGAATAACTGAAGGTGCCA+TGG 0.487818 3.3:+59659839 MS.gene63354:CDS
AAGACTGTCCATGAGCAAAC+AGG 0.492575 3.3:+59659909 MS.gene63354:CDS
AAGACAGCGGGGAGAACAAA+AGG 0.503055 3.3:-59658289 None:intergenic
ATTCTGATGCTTTCGCGACC+CGG 0.510379 3.3:-59658123 None:intergenic
AAACAACTACACAGCCATAA+GGG 0.514934 3.3:+59659713 MS.gene63354:CDS
GTTATTGCGATGCAGAGAGC+TGG 0.520901 3.3:+59658748 MS.gene63354:CDS
GCAACATGCCTGTTTGCTCA+TGG 0.521020 3.3:-59659917 None:intergenic
AGCAATTCTGTACGAGAAGA+AGG 0.521753 3.3:+59659530 MS.gene63354:CDS
TTAGTATTTGGACTAATGGT+GGG 0.527470 3.3:+59659817 MS.gene63354:CDS
GAGAACAAAAGGAAGTTTCT+CGG 0.527868 3.3:-59658278 None:intergenic
TAAGTTTGGCGTATTTGAGA+AGG 0.534048 3.3:-59658345 None:intergenic
GGAATAACTGAAGGTGCCAT+GGG 0.534697 3.3:+59659840 MS.gene63354:CDS
AGCAACTTCTTTCATACCCA+TGG 0.534773 3.3:-59659856 None:intergenic
TGAGAGGTCCAAGACGGTGC+AGG 0.535402 3.3:-59658218 None:intergenic
CACCGTCTTGGACCTCTCAC+CGG 0.539796 3.3:+59658222 MS.gene63354:CDS
TTGAGCATTCCCAAGGGTCA+CGG 0.540006 3.3:-59659655 None:intergenic
GGCTCAGGTGGCTGAAGTTG+AGG 0.543832 3.3:+59658894 MS.gene63354:CDS
GAAGAAGGCTGTGGCTCTAA+GGG 0.545005 3.3:+59658918 MS.gene63354:CDS
TGGAAGCCAAGAATCAAGCT+AGG 0.548842 3.3:+59658626 MS.gene63354:CDS
ATATGAGAAATACTCATGAC+CGG 0.550689 3.3:-59658584 None:intergenic
GATAAGGATCCCATCCATGC+AGG 0.550974 3.3:-59659942 None:intergenic
AAGCAATTGGTGGATGTGCC+CGG 0.551180 3.3:+59658565 MS.gene63354:CDS
AGTTGAGGCGAAGAAGGCTG+TGG 0.558891 3.3:+59658909 MS.gene63354:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 3.3:+59658555 MS.gene63354:CDS
ATTGAACACTAGCTTTGCTA+AGG 0.566032 3.3:-59659011 None:intergenic
AGTACACTTCTCAATGCACT+TGG 0.570595 3.3:+59659690 MS.gene63354:CDS
GAAAACTTCCTCCATCGTCA+TGG 0.571023 3.3:-59658452 None:intergenic
ATGTTGCCTCCTGCATGGAT+GGG 0.571620 3.3:+59659933 MS.gene63354:CDS
GAAGAAAGCGGCTTGGACCA+AGG 0.572319 3.3:+59658870 MS.gene63354:CDS
ATGGGTATGAAAGAAGTTGC+TGG 0.579940 3.3:+59659858 MS.gene63354:CDS
ATTGGTTCAAAATTGAGGGA+AGG 0.580771 3.3:+59658682 MS.gene63354:CDS
TGCGATGCAGAGAGCTGGAG+AGG 0.580805 3.3:+59658753 MS.gene63354:CDS
ATCGTCAACACGAGGACCAA+TGG 0.584843 3.3:-59658314 None:intergenic
TGGCTAAGAAGAAAGCGGCT+TGG 0.585206 3.3:+59658863 MS.gene63354:CDS
CTTGTTCAAGGTATCATCGA+AGG 0.588052 3.3:+59658406 MS.gene63354:CDS
ACGCTCCTTGAGCATTCCCA+AGG 0.588853 3.3:-59659662 None:intergenic
AAGACGGTGCAGGATTGACC+GGG 0.589066 3.3:-59658208 None:intergenic
GTGACCCTTGGGAATGCTCA+AGG 0.591886 3.3:+59659657 MS.gene63354:CDS
AGTATTTGGACTAATGGTGG+GGG 0.593810 3.3:+59659819 MS.gene63354:CDS
TTGTTCAAGGTATCATCGAA+GGG 0.598521 3.3:+59658407 MS.gene63354:CDS
TCTGTACGAGAAGAAGGCAG+AGG 0.600808 3.3:+59659536 MS.gene63354:CDS
GCGATGCAGAGAGCTGGAGA+GGG 0.601209 3.3:+59658754 MS.gene63354:CDS
ACTCATAGCTTGAACTTCGA+AGG 0.602674 3.3:-59658254 None:intergenic
TAGTATTTGGACTAATGGTG+GGG 0.609242 3.3:+59659818 MS.gene63354:CDS
CAGGCATGTTGCCTCCTGCA+TGG 0.611578 3.3:+59659928 MS.gene63354:CDS
CTAAGGGAAGCTGAGTTGCA+AGG 0.613783 3.3:+59658934 MS.gene63354:CDS
TTTGGGTCAAAAGACGCAGA+TGG 0.613836 3.3:+59658606 MS.gene63354:CDS
GTGTCAGAAGCTAAGATGAA+AGG 0.614706 3.3:+59658655 MS.gene63354:CDS
GTAGGTACAAGAAGCAAACT+GGG 0.614898 3.3:+59659479 MS.gene63354:intron
AGGTGTAGTTTACCGGTGAG+AGG 0.619207 3.3:-59658234 None:intergenic
TCATGGACAGTCTTAAACAA+AGG 0.619848 3.3:-59659900 None:intergenic
ATTAATGCAGAGGCGGTACG+CGG 0.619951 3.3:+59659780 MS.gene63354:CDS
TGCTGCTTCCATGACGATGG+AGG 0.622104 3.3:+59658444 MS.gene63354:CDS
TGCTAAGATTAATGCAGAGG+CGG 0.623220 3.3:+59659773 MS.gene63354:CDS
CGCTCCTTGAGCATTCCCAA+GGG 0.624114 3.3:-59659661 None:intergenic
TGAGTTGGCTAAGAAGAAAG+CGG 0.631786 3.3:+59658858 MS.gene63354:CDS
GGAAGCCAAGAATCAAGCTA+GGG 0.640292 3.3:+59658627 MS.gene63354:CDS
GCTGTACAAGAAACAGAAAG+AGG 0.643966 3.3:+59659503 MS.gene63354:CDS
GATTGCTAAGATTAATGCAG+AGG 0.644360 3.3:+59659770 MS.gene63354:CDS
TATGAGAAATACTCATGACC+GGG 0.646479 3.3:-59658583 None:intergenic
ACTTGCTGCTTCCATGACGA+TGG 0.648427 3.3:+59658441 MS.gene63354:CDS
TGGACAGTCTTAAACAAAGG+TGG 0.649112 3.3:-59659897 None:intergenic
TAGTGTTCAATATGAAACCA+AGG 0.651992 3.3:+59659023 MS.gene63354:CDS
GAAAATTGATGCAGAGACGA+AGG 0.652587 3.3:+59658726 MS.gene63354:CDS
AGTTGCAAGGTGAAGTTGAG+AGG 0.653383 3.3:+59658947 MS.gene63354:CDS
ATCAAGAATGAAGATGTACC+GGG 0.654673 3.3:+59658105 None:intergenic
GATTACTTGATGATAAATGG+TGG 0.658453 3.3:+59659735 MS.gene63354:CDS
TACCGGTGAGAGGTCCAAGA+CGG 0.667806 3.3:-59658224 None:intergenic
AAGGATCCCATCCATGCAGG+AGG 0.669522 3.3:-59659939 None:intergenic
TGAAAATACCTGTTATCACA+AGG 0.669974 3.3:-59658147 None:intergenic
AGGACCAATGGTGAAGACAG+CGG 0.683272 3.3:-59658302 None:intergenic
CTTTCCTGATCGTCAACACG+AGG 0.689163 3.3:-59658322 None:intergenic
TGTTCAAGGTATCATCGAAG+GGG 0.695602 3.3:+59658408 MS.gene63354:CDS
TGATTGGAGTGTCGATCATG+TGG 0.697521 3.3:-59658373 None:intergenic
GACCAATGGTGAAGACAGCG+GGG 0.714278 3.3:-59658300 None:intergenic
GAAAGCGGCTTGGACCAAGG+CGG 0.735762 3.3:+59658873 MS.gene63354:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATATATATATTATAT+AGG + chr3.3:59659119-59659138 MS.gene63354:intron 0.0%
!!! TATATATATTAATTTGTTGC+TGG - chr3.3:59659111-59659130 None:intergenic 15.0%
!!! TTTTTTGGTGATTAAAATTT+TGG + chr3.3:59659451-59659470 MS.gene63354:intron 15.0%
!! ATATAGGAGATTATTCAATA+TGG + chr3.3:59659135-59659154 MS.gene63354:intron 20.0%
!!! AACATTTACTTTTTAAGTGT+TGG + chr3.3:59659401-59659420 MS.gene63354:intron 20.0%
!!! TTTACTGGATTTTACTTTTT+TGG + chr3.3:59659436-59659455 MS.gene63354:intron 20.0%
! AAGATTAGTATTTGGACTAA+TGG + chr3.3:59659813-59659832 MS.gene63354:CDS 25.0%
! CATGAGTATTTCTCATATTT+GGG + chr3.3:59658589-59658608 MS.gene63354:CDS 25.0%
! TCATGAGTATTTCTCATATT+TGG + chr3.3:59658588-59658607 MS.gene63354:CDS 25.0%
! TCTTATATAGAACACTTTCA+TGG - chr3.3:59659369-59659388 None:intergenic 25.0%
! TGAAAGAAGTTATATTTGCA+TGG - chr3.3:59659059-59659078 None:intergenic 25.0%
! TTAGTCCAAATACTAATCTT+AGG - chr3.3:59659813-59659832 None:intergenic 25.0%
! TTGAACCTAAGATTAGTATT+TGG + chr3.3:59659805-59659824 MS.gene63354:CDS 25.0%
!! ATTAAAATTTTGGATGGTGT+AGG + chr3.3:59659461-59659480 MS.gene63354:intron 25.0%
!! TGAAATTGGTTCAAAATTGA+GGG + chr3.3:59658678-59658697 MS.gene63354:CDS 25.0%
!!! TGGCTATTTGATAATTTTAC+TGG + chr3.3:59659421-59659440 MS.gene63354:intron 25.0%
!!! TTGGTGATTAAAATTTTGGA+TGG + chr3.3:59659455-59659474 MS.gene63354:intron 25.0%
AACAAGTTCATTAACGTGAT+TGG - chr3.3:59658392-59658411 None:intergenic 30.0%
ACAAGACAAAACAATTCAAG+TGG - chr3.3:59659344-59659363 None:intergenic 30.0%
ATATGAGAAATACTCATGAC+CGG - chr3.3:59658587-59658606 None:intergenic 30.0%
ATCATGTGGTGAAATAAGTT+TGG - chr3.3:59658362-59658381 None:intergenic 30.0%
ATTAGTATTTGGACTAATGG+TGG + chr3.3:59659816-59659835 MS.gene63354:CDS 30.0%
GCTATATGCAAAGAAAAAAG+AGG + chr3.3:59659623-59659642 MS.gene63354:CDS 30.0%
TAGTGTTCAATATGAAACCA+AGG + chr3.3:59659023-59659042 MS.gene63354:CDS 30.0%
TATCATCAAGTAATCCCTTA+TGG - chr3.3:59659730-59659749 None:intergenic 30.0%
TCAAACTCAAAAAGAAAGCA+TGG + chr3.3:59658179-59658198 MS.gene63354:CDS 30.0%
TGAAAATACCTGTTATCACA+AGG - chr3.3:59658150-59658169 None:intergenic 30.0%
TTAGTATTTGGACTAATGGT+GGG + chr3.3:59659817-59659836 MS.gene63354:CDS 30.0%
! AGGGATTACTTGATGATAAA+TGG + chr3.3:59659732-59659751 MS.gene63354:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG + chr3.3:59659735-59659754 MS.gene63354:CDS 30.0%
! GTGAAATTGGTTCAAAATTG+AGG + chr3.3:59658677-59658696 MS.gene63354:CDS 30.0%
! TTAAGAGCTTTTGTCAGATA+AGG - chr3.3:59659961-59659980 None:intergenic 30.0%
!! CAACTTGAGTTGAATCAATT+CGG + chr3.3:59658517-59658536 MS.gene63354:CDS 30.0%
AAACAACTACACAGCCATAA+GGG + chr3.3:59659713-59659732 MS.gene63354:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG + chr3.3:59658552-59658571 MS.gene63354:CDS 35.0%
GAGAACAAAAGGAAGTTTCT+CGG - chr3.3:59658281-59658300 None:intergenic 35.0%
GATGAGAACAATGTCTGTTT+GGG + chr3.3:59659180-59659199 MS.gene63354:intron 35.0%
GATTGCTAAGATTAATGCAG+AGG + chr3.3:59659770-59659789 MS.gene63354:CDS 35.0%
GCTAAGATGAAAGGTGAAAT+TGG + chr3.3:59658664-59658683 MS.gene63354:CDS 35.0%
TAAGTTTGGCGTATTTGAGA+AGG - chr3.3:59658348-59658367 None:intergenic 35.0%
TAGTATTTGGACTAATGGTG+GGG + chr3.3:59659818-59659837 MS.gene63354:CDS 35.0%
TATGAGAAATACTCATGACC+GGG - chr3.3:59658586-59658605 None:intergenic 35.0%
TCAGAATACCTTGTGATAAC+AGG + chr3.3:59658139-59658158 MS.gene63354:CDS 35.0%
TCATGGACAGTCTTAAACAA+AGG - chr3.3:59659903-59659922 None:intergenic 35.0%
TGATGAGAACAATGTCTGTT+TGG + chr3.3:59659179-59659198 MS.gene63354:intron 35.0%
! ATTGGTTCAAAATTGAGGGA+AGG + chr3.3:59658682-59658701 MS.gene63354:CDS 35.0%
! CACGTTAATGAACTTGTTCA+AGG + chr3.3:59658394-59658413 MS.gene63354:CDS 35.0%
! TTGTTCAAGGTATCATCGAA+GGG + chr3.3:59658407-59658426 MS.gene63354:CDS 35.0%
!! AAGAAAGCATGGATTTTTCC+CGG + chr3.3:59658190-59658209 MS.gene63354:CDS 35.0%
!! ATTGAACACTAGCTTTGCTA+AGG - chr3.3:59659014-59659033 None:intergenic 35.0%
!! GGTTTTTGAGAATCAAAGAG+AGG + chr3.3:59658813-59658832 MS.gene63354:intron 35.0%
ACTCATAGCTTGAACTTCGA+AGG - chr3.3:59658257-59658276 None:intergenic 40.0%
AGAAGGAGGGAATTAAAGTG+AGG + chr3.3:59658779-59658798 MS.gene63354:intron 40.0%
AGCAACTTCTTTCATACCCA+TGG - chr3.3:59659859-59659878 None:intergenic 40.0%
AGCAATTCTGTACGAGAAGA+AGG + chr3.3:59659530-59659549 MS.gene63354:CDS 40.0%
AGTACACTTCTCAATGCACT+TGG + chr3.3:59659690-59659709 MS.gene63354:CDS 40.0%
AGTATTTGGACTAATGGTGG+GGG + chr3.3:59659819-59659838 MS.gene63354:CDS 40.0%
ATGGGTATGAAAGAAGTTGC+TGG + chr3.3:59659858-59659877 MS.gene63354:CDS 40.0%
CAATGTCTGTTTGGGTGTTT+AGG + chr3.3:59659188-59659207 MS.gene63354:intron 40.0%
GAAAATTGATGCAGAGACGA+AGG + chr3.3:59658726-59658745 MS.gene63354:CDS 40.0%
GAAACAACTACACAGCCATA+AGG + chr3.3:59659712-59659731 MS.gene63354:CDS 40.0%
GCAAAGAAAAAAGAGGCAGA+GGG + chr3.3:59659630-59659649 MS.gene63354:CDS 40.0%
GCTGTACAAGAAACAGAAAG+AGG + chr3.3:59659503-59659522 MS.gene63354:CDS 40.0%
GGCTTAAGCTAATTCTGAGT+TGG + chr3.3:59658843-59658862 MS.gene63354:intron 40.0%
GTAGGTACAAGAAGCAAACT+GGG + chr3.3:59659479-59659498 MS.gene63354:intron 40.0%
GTGTCAGAAGCTAAGATGAA+AGG + chr3.3:59658655-59658674 MS.gene63354:CDS 40.0%
TAAAGTGAGGACTGAAGTGA+AGG + chr3.3:59658792-59658811 MS.gene63354:intron 40.0%
TGCAAAGAAAAAAGAGGCAG+AGG + chr3.3:59659629-59659648 MS.gene63354:CDS 40.0%
TGCGAATGTTAAGCAATTGG+TGG + chr3.3:59658555-59658574 MS.gene63354:CDS 40.0%
TGCTAAGATTAATGCAGAGG+CGG + chr3.3:59659773-59659792 MS.gene63354:CDS 40.0%
TGGACAGTCTTAAACAAAGG+TGG - chr3.3:59659900-59659919 None:intergenic 40.0%
TGTAGGTACAAGAAGCAAAC+TGG + chr3.3:59659478-59659497 MS.gene63354:intron 40.0%
! ACTTCGAAGGTGTAGTTTAC+CGG - chr3.3:59658244-59658263 None:intergenic 40.0%
! CTTGTTCAAGGTATCATCGA+AGG + chr3.3:59658406-59658425 MS.gene63354:CDS 40.0%
! CTTTGCATATAGCTCTGCTT+CGG - chr3.3:59659616-59659635 None:intergenic 40.0%
! TGAGTTGGCTAAGAAGAAAG+CGG + chr3.3:59658858-59658877 MS.gene63354:CDS 40.0%
! TGCATGGACTAAACTAACCT+TGG - chr3.3:59659043-59659062 None:intergenic 40.0%
! TGTTCAAGGTATCATCGAAG+GGG + chr3.3:59658408-59658427 MS.gene63354:CDS 40.0%
AAGACTGTCCATGAGCAAAC+AGG + chr3.3:59659909-59659928 MS.gene63354:CDS 45.0%
AATGCTTAAGCATGCCCCAA+GGG - chr3.3:59659284-59659303 None:intergenic 45.0%
ACCAACATAGTCACGCTCAA+TGG + chr3.3:59659245-59659264 MS.gene63354:intron 45.0%
AGTTGCAAGGTGAAGTTGAG+AGG + chr3.3:59658947-59658966 MS.gene63354:CDS 45.0%
CCAACATAGTCACGCTCAAT+GGG + chr3.3:59659246-59659265 MS.gene63354:intron 45.0%
GAAAACTTCCTCCATCGTCA+TGG - chr3.3:59658455-59658474 None:intergenic 45.0%
GAATCAAAGAGAGGCTGAAG+TGG + chr3.3:59658822-59658841 MS.gene63354:intron 45.0%
GGAAGCCAAGAATCAAGCTA+GGG + chr3.3:59658627-59658646 MS.gene63354:CDS 45.0%
GTCAACCCTAGCTTGATTCT+TGG - chr3.3:59658635-59658654 None:intergenic 45.0%
TGGAAGCCAAGAATCAAGCT+AGG + chr3.3:59658626-59658645 MS.gene63354:CDS 45.0%
TTTGGGTCAAAAGACGCAGA+TGG + chr3.3:59658606-59658625 MS.gene63354:CDS 45.0%
! AATGGTGGGGGAATAACTGA+AGG + chr3.3:59659831-59659850 MS.gene63354:CDS 45.0%
! ACGATGGAGGAAGTTTTCAG+AGG + chr3.3:59658457-59658476 MS.gene63354:CDS 45.0%
!! GGAATAACTGAAGGTGCCAT+GGG + chr3.3:59659840-59659859 MS.gene63354:CDS 45.0%
!! TGATTGGAGTGTCGATCATG+TGG - chr3.3:59658376-59658395 None:intergenic 45.0%
AAGACAGCGGGGAGAACAAA+AGG - chr3.3:59658292-59658311 None:intergenic 50.0%
ACATAGTCACGCTCAATGGG+TGG + chr3.3:59659249-59659268 MS.gene63354:intron 50.0%
AGGACCAATGGTGAAGACAG+CGG - chr3.3:59658305-59658324 None:intergenic 50.0%
AGGTGTAGTTTACCGGTGAG+AGG - chr3.3:59658237-59658256 None:intergenic 50.0%
ATCGTCAACACGAGGACCAA+TGG - chr3.3:59658317-59658336 None:intergenic 50.0%
ATGTTGCCTCCTGCATGGAT+GGG + chr3.3:59659933-59659952 MS.gene63354:CDS 50.0%
ATTAATGCAGAGGCGGTACG+CGG + chr3.3:59659780-59659799 MS.gene63354:CDS 50.0%
ATTCTGATGCTTTCGCGACC+CGG - chr3.3:59658126-59658145 None:intergenic 50.0%
CCCATTGAGCGTGACTATGT+TGG - chr3.3:59659249-59659268 None:intergenic 50.0%
CTAAGGGAAGCTGAGTTGCA+AGG + chr3.3:59658934-59658953 MS.gene63354:CDS 50.0%
CTTTCCTGATCGTCAACACG+AGG - chr3.3:59658325-59658344 None:intergenic 50.0%
GAATGCTTAAGCATGCCCCA+AGG - chr3.3:59659285-59659304 None:intergenic 50.0%
GATAAGGATCCCATCCATGC+AGG - chr3.3:59659945-59659964 None:intergenic 50.0%
GCAACATGCCTGTTTGCTCA+TGG - chr3.3:59659920-59659939 None:intergenic 50.0%
GGGTGGTAATAAGTGCCCTT+GGG + chr3.3:59659266-59659285 MS.gene63354:intron 50.0%
GGTGGTAATAAGTGCCCTTG+GGG + chr3.3:59659267-59659286 MS.gene63354:intron 50.0%
GTTATTGCGATGCAGAGAGC+TGG + chr3.3:59658748-59658767 MS.gene63354:CDS 50.0%
TCTGTACGAGAAGAAGGCAG+AGG + chr3.3:59659536-59659555 MS.gene63354:CDS 50.0%
TGGCTAAGAAGAAAGCGGCT+TGG + chr3.3:59658863-59658882 MS.gene63354:CDS 50.0%
TGGGTGGTAATAAGTGCCCT+TGG + chr3.3:59659265-59659284 MS.gene63354:intron 50.0%
TTGAGCATTCCCAAGGGTCA+CGG - chr3.3:59659658-59659677 None:intergenic 50.0%
! AAGCAATTGGTGGATGTGCC+CGG + chr3.3:59658565-59658584 MS.gene63354:CDS 50.0%
! ACTTGCTGCTTCCATGACGA+TGG + chr3.3:59658441-59658460 MS.gene63354:CDS 50.0%
!! GAAGAAGGCTGTGGCTCTAA+GGG + chr3.3:59658918-59658937 MS.gene63354:CDS 50.0%
!! GGGAATAACTGAAGGTGCCA+TGG + chr3.3:59659839-59659858 MS.gene63354:CDS 50.0%
AAGACGGTGCAGGATTGACC+GGG - chr3.3:59658211-59658230 None:intergenic 55.0%
AAGGATCCCATCCATGCAGG+AGG - chr3.3:59659942-59659961 None:intergenic 55.0%
ACGCTCCTTGAGCATTCCCA+AGG - chr3.3:59659665-59659684 None:intergenic 55.0%
AGTTGAGGCGAAGAAGGCTG+TGG + chr3.3:59658909-59658928 MS.gene63354:CDS 55.0%
CAAGACGGTGCAGGATTGAC+CGG - chr3.3:59658212-59658231 None:intergenic 55.0%
CATGTTGCCTCCTGCATGGA+TGG + chr3.3:59659932-59659951 MS.gene63354:CDS 55.0%
CGCTCCTTGAGCATTCCCAA+GGG - chr3.3:59659664-59659683 None:intergenic 55.0%
GACCAATGGTGAAGACAGCG+GGG - chr3.3:59658303-59658322 None:intergenic 55.0%
GGACCAATGGTGAAGACAGC+GGG - chr3.3:59658304-59658323 None:intergenic 55.0%
GGCTGAAGTTGAGGCGAAGA+AGG + chr3.3:59658903-59658922 MS.gene63354:CDS 55.0%
GTGACCCTTGGGAATGCTCA+AGG + chr3.3:59659657-59659676 MS.gene63354:CDS 55.0%
TACCGGTGAGAGGTCCAAGA+CGG - chr3.3:59658227-59658246 None:intergenic 55.0%
! CGAAGAAGGCTGTGGCTCTA+AGG + chr3.3:59658917-59658936 MS.gene63354:CDS 55.0%
! GAAGAAAGCGGCTTGGACCA+AGG + chr3.3:59658870-59658889 MS.gene63354:CDS 55.0%
! TGCTGCTTCCATGACGATGG+AGG + chr3.3:59658444-59658463 MS.gene63354:CDS 55.0%
! TGGTCCTCGTGTTGACGATC+AGG + chr3.3:59658318-59658337 MS.gene63354:CDS 55.0%
AGCTGGAGAGGGTGAGAAGG+AGG + chr3.3:59658765-59658784 MS.gene63354:intron 60.0%
CACCGTCTTGGACCTCTCAC+CGG + chr3.3:59658222-59658241 MS.gene63354:CDS 60.0%
CAGAGGGAACCGTGACCCTT+GGG + chr3.3:59659646-59659665 MS.gene63354:CDS 60.0%
CAGGCATGTTGCCTCCTGCA+TGG + chr3.3:59659928-59659947 MS.gene63354:CDS 60.0%
CGGTCAATCCTGCACCGTCT+TGG + chr3.3:59658210-59658229 MS.gene63354:CDS 60.0%
CTCCCCGCTGTCTTCACCAT+TGG + chr3.3:59658298-59658317 MS.gene63354:CDS 60.0%
GAGAGCTGGAGAGGGTGAGA+AGG + chr3.3:59658762-59658781 MS.gene63354:intron 60.0%
GCGATGCAGAGAGCTGGAGA+GGG + chr3.3:59658754-59658773 MS.gene63354:CDS 60.0%
GCTGGAGAGGGTGAGAAGGA+GGG + chr3.3:59658766-59658785 MS.gene63354:intron 60.0%
GGCTCAGGTGGCTGAAGTTG+AGG + chr3.3:59658894-59658913 MS.gene63354:CDS 60.0%
TGAGAGGTCCAAGACGGTGC+AGG - chr3.3:59658221-59658240 None:intergenic 60.0%
TGCGATGCAGAGAGCTGGAG+AGG + chr3.3:59658753-59658772 MS.gene63354:CDS 60.0%
! GAAAGCGGCTTGGACCAAGG+CGG + chr3.3:59658873-59658892 MS.gene63354:CDS 60.0%
GCAGAGGGAACCGTGACCCT+TGG + chr3.3:59659645-59659664 MS.gene63354:CDS 65.0%
TTCAGCCACCTGAGCCGCCT+TGG - chr3.3:59658890-59658909 None:intergenic 65.0%
TTGGACCAAGGCGGCTCAGG+TGG + chr3.3:59658882-59658901 MS.gene63354:CDS 65.0%
GGCTTGGACCAAGGCGGCTC+AGG + chr3.3:59658879-59658898 MS.gene63354:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 59658112 59659980 59658112 ID=MS.gene63354
chr3.3 mRNA 59658112 59659980 59658112 ID=MS.gene63354.t1;Parent=MS.gene63354
chr3.3 exon 59658112 59658775 59658112 ID=MS.gene63354.t1.exon1;Parent=MS.gene63354.t1
chr3.3 CDS 59658112 59658775 59658112 ID=cds.MS.gene63354.t1;Parent=MS.gene63354.t1
chr3.3 exon 59658851 59659044 59658851 ID=MS.gene63354.t1.exon2;Parent=MS.gene63354.t1
chr3.3 CDS 59658851 59659044 59658851 ID=cds.MS.gene63354.t1;Parent=MS.gene63354.t1
chr3.3 exon 59659483 59659980 59659483 ID=MS.gene63354.t1.exon3;Parent=MS.gene63354.t1
chr3.3 CDS 59659483 59659980 59659483 ID=cds.MS.gene63354.t1;Parent=MS.gene63354.t1
Gene Sequence

>MS.gene63354

ATGAAGATGTACCGGGTCGCGAAAGCATCAGAATACCTTGTGATAACAGGTATTTTCATCAAAGACATCAAACTCAAAAAGAAAGCATGGATTTTTCCCGGTCAATCCTGCACCGTCTTGGACCTCTCACCGGTAAACTACACCTTCGAAGTTCAAGCTATGAGTGCCGAGAAACTTCCTTTTGTTCTCCCCGCTGTCTTCACCATTGGTCCTCGTGTTGACGATCAGGAAAGCCTTCTCAAATACGCCAAACTTATTTCACCACATGATCGACACTCCAATCACGTTAATGAACTTGTTCAAGGTATCATCGAAGGGGAGACACGTGTACTTGCTGCTTCCATGACGATGGAGGAAGTTTTCAGAGGAACAAAACAGTTTAAACAAGAAGTATTCGACAAAGTTCAACTTGAGTTGAATCAATTCGGATTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCCCGGTCATGAGTATTTCTCATATTTGGGTCAAAAGACGCAGATGGAAGCCAAGAATCAAGCTAGGGTTGACGTGTCAGAAGCTAAGATGAAAGGTGAAATTGGTTCAAAATTGAGGGAAGGACAAACGCTGCAGAATGCAGCGAAAATTGATGCAGAGACGAAGGTTATTGCGATGCAGAGAGCTGGAGAGGGTGAGAAGGAGGGAATTAAAGTGAGGACTGAAGTGAAGGTTTTTGAGAATCAAAGAGAGGCTGAAGTGGCTTAAGCTAATTCTGAGTTGGCTAAGAAGAAAGCGGCTTGGACCAAGGCGGCTCAGGTGGCTGAAGTTGAGGCGAAGAAGGCTGTGGCTCTAAGGGAAGCTGAGTTGCAAGGTGAAGTTGAGAGGATGAATGCTTTGACTACAACTGAGAAGCTTAAAGCAGAGTTCCTTAGCAAAGCTAGTGTTCAATATGAAACCAAGGTTAGTTTAGTCCATGCAAATATAACTTCTTTCATTATATTCTTATGCAATTTTTTTCTTTCACCAGCAACAAATTAATATATATATATTATATAGGAGATTATTCAATATGGTTAAAGTTATTTTCATATATGTGATGAGAACAATGTCTGTTTGGGTGTTTAGGTTCATTTTATTGCTCTCTGTCACTTTCAAGTTTGACCAACATAGTCACGCTCAATGGGTGGTAATAAGTGCCCTTGGGGCATGCTTAAGCATTCAAAAGTAGTAATCTAAAAAAAAAAAATTAGACTACTCCACTTGAATTGTTTTGTCTTGTCGCCATGAAAGTGTTCTATATAAGATGAATGATTTTCAACATTTACTTTTTAAGTGTTGGCTATTTGATAATTTTACTGGATTTTACTTTTTTGGTGATTAAAATTTTGGATGGTGTAGGTACAAGAAGCAAACTGGGAGCTGTACAAGAAACAGAAAGAGGCTGAAGCAATTCTGTACGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATCAGCAGATGCAACATTTTATGCTCGCAAGCAAGAAGCCGAAGCAGAGCTATATGCAAAGAAAAAAGAGGCAGAGGGAACCGTGACCCTTGGGAATGCTCAAGGAGCGTATGTAAGTACACTTCTCAATGCACTTGGAAACAACTACACAGCCATAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCAGAGGCGGTACGCGGACTTGAACCTAAGATTAGTATTTGGACTAATGGTGGGGGAATAACTGAAGGTGCCATGGGTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTTTGTTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCTGCATGGATGGGATCCTTATCTGACAAAAGCTCTTAA
Protein sequence

>MS.gene63354.t1

MKMYRVAKASEYLVITGIFIKDIKLKKKAWIFPGQSCTVLDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDRHSNHVNELVQGIIEGETRVLAASMTMEEVFRGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAKNQARVDVSEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEANSELAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKAEFLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKASADATFYARKQEAEAELYAKKKEAEGTVTLGNAQGAYVSTLLNALGNNYTAIRDYLMINGGMFQEIAKINAEAVRGLEPKISIWTNGGGITEGAMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGSLSDKSS