AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004728

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004728.t1 MTR_1g073400 90.795 478 12 2 1 450 1 474 0.0 867
MS.gene004728.t1 MTR_3g106480 86.611 478 36 1 1 450 1 478 0.0 838
MS.gene004728.t1 MTR_1g071130 91.009 456 28 4 1 450 1 449 0.0 829
MS.gene004728.t1 MTR_1g115145 88.866 476 24 3 1 450 1 473 0.0 822
MS.gene004728.t1 MTR_3g106420 85.983 478 39 1 1 450 3 480 0.0 806
MS.gene004728.t1 MTR_3g106485 86.402 478 33 3 1 450 1 474 0.0 803
MS.gene004728.t1 MTR_1g083190 83.473 478 43 2 1 450 3 472 0.0 768
MS.gene004728.t1 MTR_3g106430 82.600 477 51 4 1 449 1 473 0.0 768
MS.gene004728.t1 MTR_3g106470 80.000 455 51 3 1 449 32 452 0.0 693
MS.gene004728.t1 MTR_8g063830 72.258 465 60 7 1 436 1 425 0.0 612
MS.gene004728.t1 MTR_3g065540 66.087 115 12 1 40 154 14 101 7.37e-42 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004728.t1 AT5G25260 70.886 474 98 3 1 446 1 462 0.0 683
MS.gene004728.t1 AT5G25250 70.613 473 97 3 1 445 1 459 0.0 671
MS.gene004728.t1 AT5G64870 68.008 472 112 2 2 445 3 463 0.0 615

Find 108 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAATATTTCTCATATTT+AGG 0.103686 1.1:-55455330 MS.gene004728:CDS
TTGAACCAAAGATTAGTATT+TGG 0.248544 1.1:-55454206 MS.gene004728:CDS
CGGAATAACGGAAGGATCTA+TGG 0.261895 1.1:-55454160 MS.gene004728:CDS
CTGCTAAAGCTGTGGCTCTA+AGG 0.272737 1.1:-55455048 MS.gene004728:CDS
AGGGATTACTTGATGATAAA+TGG 0.295084 1.1:-55454279 MS.gene004728:CDS
TTAGTCCAAATACTAATCTT+TGG 0.297211 1.1:+55454201 None:intergenic
ATGAATATTTCTCATATTTA+GGG 0.305111 1.1:-55455329 MS.gene004728:CDS
GTGAAGTTGGCTCGAAATCT+AGG 0.314615 1.1:-55455242 MS.gene004728:CDS
TTTAGGTACAAGAGGCTAAC+TGG 0.315931 1.1:-55454533 MS.gene004728:intron
CTTCCCGCTGTCTTCACAAT+TGG 0.324959 1.1:-55455621 MS.gene004728:CDS
CAACTTGAACTAAACCAATT+CGG 0.326573 1.1:-55455402 MS.gene004728:CDS
TCTAAGGGAAGCTGAGTTGC+AGG 0.329397 1.1:-55455032 MS.gene004728:CDS
CATGTTGCCTCCTGCTTGGA+TGG 0.342126 1.1:-55454067 MS.gene004728:CDS
TGTAAATCAATAACCCGAAT+TGG 0.349196 1.1:+55455388 None:intergenic
GAAACGACTACACAGCTCTA+AGG 0.361629 1.1:-55454299 MS.gene004728:CDS
AAGATTAGTATTTGGACTAA+TGG 0.364380 1.1:-55454198 MS.gene004728:CDS
CAATGCGAATGTTAAGCAAT+TGG 0.388576 1.1:-55455367 MS.gene004728:CDS
ATCAAAATCAAACGATGTAC+AGG 0.393868 1.1:-55455815 None:intergenic
TGGTCCTCGTGTTGACGATC+AGG 0.407219 1.1:-55455601 MS.gene004728:CDS
TCAAACTTGCAAAGAAATCA+TGG 0.416941 1.1:-55455740 MS.gene004728:CDS
AACTTGAACTAAACCAATTC+GGG 0.426389 1.1:-55455401 MS.gene004728:CDS
TCCGAGTACCTGGTGATAAC+CGG 0.437519 1.1:-55455780 MS.gene004728:CDS
GATGTCTTCGATTCCAGCTC+CGG 0.437947 1.1:+55455761 None:intergenic
AGCAATTCTGTATGAGAAGA+AGG 0.462456 1.1:-55454481 MS.gene004728:CDS
AAATCAGTTTAGCGTATTTG+AGG 0.472961 1.1:+55455571 None:intergenic
GAAGAAAGCGGCGTGGACTA+AGG 0.476838 1.1:-55455095 MS.gene004728:CDS
TTTAGGGCAAAAGAAGCAAA+TGG 0.491891 1.1:-55455313 MS.gene004728:CDS
AAGACTGTCCATGAGCAAAC+AGG 0.492575 1.1:-55454090 MS.gene004728:CDS
TGGAAGCAGCGAATCAAGCT+AGG 0.499263 1.1:-55455293 MS.gene004728:CDS
TGCTAAAGCTGTGGCTCTAA+GGG 0.501192 1.1:-55455047 MS.gene004728:CDS
CAGGCATGTTGCCTCCTGCT+TGG 0.503335 1.1:-55454071 MS.gene004728:CDS
TGCTTCGATGACCATGGAAG+AGG 0.508530 1.1:-55455472 MS.gene004728:CDS
AGCAAAAGCATCCGAGTACC+TGG 0.509436 1.1:-55455790 MS.gene004728:CDS
GTGGACTAAGGCGGCTCAAG+TGG 0.516297 1.1:-55455083 MS.gene004728:CDS
GCTAAGATGAAAGGTGAAGT+TGG 0.517494 1.1:-55455255 MS.gene004728:CDS
CATGTCAAGGAACTAGTTCA+AGG 0.517510 1.1:-55455525 MS.gene004728:CDS
GCAACATGCCTGTTTGCTCA+TGG 0.521020 1.1:+55454082 None:intergenic
ATGTTGCCTCCTGCTTGGAT+GGG 0.522936 1.1:-55454066 MS.gene004728:CDS
GTTATTGCTATGCAGAGGGC+TGG 0.525373 1.1:-55455171 MS.gene004728:CDS
TACCTGGTGATAACCGGAGC+TGG 0.525647 1.1:-55455774 MS.gene004728:CDS
AAGCAATTGGTGGATGTGCC+TGG 0.526695 1.1:-55455354 MS.gene004728:CDS
TCCTCTGAAAACCTCTTCCA+TGG 0.530446 1.1:+55455461 None:intergenic
ACTTCGAAGGTATAGTTCAC+AGG 0.531584 1.1:+55455678 None:intergenic
TGGCAATGGAGGCGGAATAA+CGG 0.532188 1.1:-55454172 MS.gene004728:CDS
CGAAAGTTATTGCTATGCAG+AGG 0.532272 1.1:-55455176 MS.gene004728:CDS
GGCTCAAGTGGCTGAAGTCG+AGG 0.533715 1.1:-55455071 MS.gene004728:CDS
ATTAGTATTTGGACTAATGG+AGG 0.534214 1.1:-55454195 MS.gene004728:CDS
AGGATCTATGGGCATGAAAG+AGG 0.537670 1.1:-55454148 MS.gene004728:CDS
TTCCAGCTCCGGTTATCACC+AGG 0.538435 1.1:+55455772 None:intergenic
AATGGAGGTGGCAATGGAGG+CGG 0.538662 1.1:-55454180 MS.gene004728:CDS
TGGTGGCATGTTTCAAGAGA+TGG 0.539247 1.1:-55454259 MS.gene004728:CDS
TCCGGTTATCACCAGGTACT+CGG 0.542656 1.1:+55455779 None:intergenic
TGGACTAATGGAGGTGGCAA+TGG 0.546081 1.1:-55454186 MS.gene004728:CDS
GCAGAAGGGATTGTGACACT+TGG 0.546780 1.1:-55454366 MS.gene004728:CDS
ATGGGCATGAAAGAGGTTGC+TGG 0.547263 1.1:-55454141 MS.gene004728:CDS
GATAAGGTTCCCATCCAAGC+AGG 0.550974 1.1:+55454057 None:intergenic
GGAAGCTGAGTTGCAGGGTG+AGG 0.555691 1.1:-55455026 MS.gene004728:CDS
TTGGATGGTTTAGGTACAAG+AGG 0.561865 1.1:-55454541 MS.gene004728:intron
CAAACTTGCAAAGAAATCAT+GGG 0.562132 1.1:-55455739 MS.gene004728:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 1.1:-55455364 MS.gene004728:CDS
GGACCAATTGTGAAGACAGC+GGG 0.564372 1.1:+55455618 None:intergenic
GGAATAACGGAAGGATCTAT+GGG 0.567283 1.1:-55454159 MS.gene004728:CDS
ACTAATGGAGGTGGCAATGG+AGG 0.570113 1.1:-55454183 MS.gene004728:CDS
AGTACACTTCTCAATGCACT+TGG 0.570595 1.1:-55454321 MS.gene004728:CDS
CCTCGCTGCTTCGATGACCA+TGG 0.570615 1.1:-55455478 MS.gene004728:CDS
TCATGGACAGTCTTAAATAG+AGG 0.570661 1.1:+55454099 None:intergenic
AAGACGGTGCAAGATTGACC+AGG 0.574032 1.1:+55455711 None:intergenic
TTAGGTACAAGAGGCTAACT+GGG 0.579786 1.1:-55454532 MS.gene004728:intron
TGAAGTTGGCTCGAAATCTA+GGG 0.579862 1.1:-55455241 MS.gene004728:CDS
GTGACACTTGGGAATGCTCA+AGG 0.583871 1.1:-55454354 MS.gene004728:CDS
AGTATTTGGACTAATGGAGG+TGG 0.584442 1.1:-55454192 MS.gene004728:CDS
TCAGTTTAGCGTATTTGAGG+AGG 0.587438 1.1:+55455574 None:intergenic
AGTTGCAGGGTGAGGTTGAG+AGG 0.589803 1.1:-55455018 MS.gene004728:CDS
AAACGACTACACAGCTCTAA+GGG 0.592700 1.1:-55454298 MS.gene004728:CDS
CAGAAGGGATTGTGACACTT+GGG 0.598815 1.1:-55454365 MS.gene004728:CDS
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 1.1:-55454475 MS.gene004728:CDS
ACTCATGGCTTGAACTTCGA+AGG 0.601319 1.1:+55455665 None:intergenic
CTAGTTCAAGGAATCATCGA+AGG 0.612313 1.1:-55455513 MS.gene004728:CDS
GTGGCAGAAGCTAAGATGAA+AGG 0.614706 1.1:-55455264 MS.gene004728:CDS
CACAGGTGAGAGGTCGAAGA+CGG 0.624112 1.1:+55455695 None:intergenic
AGGTATAGTTCACAGGTGAG+AGG 0.626851 1.1:+55455685 None:intergenic
AAGACAGCGGGAAGAACGAA+AGG 0.630029 1.1:+55455630 None:intergenic
GGAAGCAGCGAATCAAGCTA+GGG 0.630802 1.1:-55455292 MS.gene004728:CDS
TAGTTCAAGGAATCATCGAA+GGG 0.630851 1.1:-55455512 MS.gene004728:CDS
GAAAGTTATTGCTATGCAGA+GGG 0.630907 1.1:-55455175 MS.gene004728:CDS
CTAAGGGAAGCTGAGTTGCA+GGG 0.631632 1.1:-55455031 MS.gene004728:CDS
ATCAATGCTGAGGCAGTACG+TGG 0.635980 1.1:-55454231 MS.gene004728:CDS
TATGAGAAATATTCATGACC+AGG 0.636387 1.1:+55455336 None:intergenic
TCAAAATCAAACGATGTACA+GGG 0.641066 1.1:-55455814 None:intergenic
TAGTGTTGAGTATGAAACCA+AGG 0.644971 1.1:-55454942 MS.gene004728:intron
TGGACAGTCTTAAATAGAGG+TGG 0.647512 1.1:+55454102 None:intergenic
GAAAGCGGCGTGGACTAAGG+CGG 0.652094 1.1:-55455092 MS.gene004728:CDS
AATGGAGGCGGAATAACGGA+AGG 0.652839 1.1:-55454168 MS.gene004728:CDS
TAAACTCTCGAATCATGTCA+AGG 0.655320 1.1:-55455538 MS.gene004728:CDS
GATTACTTGATGATAAATGG+TGG 0.658453 1.1:-55454276 MS.gene004728:CDS
AGTCGAGGCTGCTAAAGCTG+TGG 0.666475 1.1:-55455056 MS.gene004728:CDS
AAGGTTCCCATCCAAGCAGG+AGG 0.669932 1.1:+55454060 None:intergenic
GTTGGCTCGAAATCTAGGGA+AGG 0.671253 1.1:-55455237 MS.gene004728:CDS
TGATTCGAGAGTTTATCATG+AGG 0.680428 1.1:+55455546 None:intergenic
GATGGCTAAGATCAATGCTG+AGG 0.682349 1.1:-55454241 MS.gene004728:CDS
GAATCAAGCTAGGGTTGACG+TGG 0.682564 1.1:-55455283 MS.gene004728:CDS
CTTTCCTGATCGTCAACACG+AGG 0.689163 1.1:+55455597 None:intergenic
AGAGTTGGCGAAGAAGAAAG+CGG 0.695769 1.1:-55455107 MS.gene004728:CDS
AGACGGTGCAAGATTGACCA+GGG 0.716272 1.1:+55455712 None:intergenic
TGGCGAAGAAGAAAGCGGCG+TGG 0.717862 1.1:-55455102 MS.gene004728:CDS
AGTTCAAGGAATCATCGAAG+GGG 0.721843 1.1:-55455511 MS.gene004728:CDS
AGGACCAATTGTGAAGACAG+CGG 0.726189 1.1:+55455617 None:intergenic
CCATGGTCATCGAAGCAGCG+AGG 0.728643 1.1:+55455478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTATTTTTTAAATAC+TGG - chr1.1:55455270-55455289 MS.gene004728:CDS 10.0%
!! ATGAATATTTCTCATATTTA+GGG - chr1.1:55454513-55454532 MS.gene004728:CDS 15.0%
!! CATTATAAAACTAAATCATA+TGG - chr1.1:55455149-55455168 MS.gene004728:intron 15.0%
!!! TGTTCTAAATTAATTTTGTT+AGG - chr1.1:55455205-55455224 MS.gene004728:CDS 15.0%
!!! TTAATATGTTATATGGATTA+TGG - chr1.1:55455073-55455092 MS.gene004728:CDS 15.0%
!! CATGAATATTTCTCATATTT+AGG - chr1.1:55454512-55454531 MS.gene004728:CDS 20.0%
!!! TTAAATACTGGTTGAAATTT+TGG - chr1.1:55455282-55455301 MS.gene004728:CDS 20.0%
! AAAGAAGAGTTATATTTGCA+TGG + chr1.1:55454931-55454950 None:intergenic 25.0%
! AAGATTAGTATTTGGACTAA+TGG - chr1.1:55455644-55455663 MS.gene004728:CDS 25.0%
! TTAGTCCAAATACTAATCTT+TGG + chr1.1:55455644-55455663 None:intergenic 25.0%
! TTGAACCAAAGATTAGTATT+TGG - chr1.1:55455636-55455655 MS.gene004728:CDS 25.0%
AAATCAGTTTAGCGTATTTG+AGG + chr1.1:55454274-55454293 None:intergenic 30.0%
AACTTGAACTAAACCAATTC+GGG - chr1.1:55454441-55454460 MS.gene004728:CDS 30.0%
ATTAGTATTTGGACTAATGG+AGG - chr1.1:55455647-55455666 MS.gene004728:CDS 30.0%
CAAACTTGCAAAGAAATCAT+GGG - chr1.1:55454103-55454122 MS.gene004728:CDS 30.0%
CAACTTGAACTAAACCAATT+CGG - chr1.1:55454440-55454459 MS.gene004728:CDS 30.0%
GCTATACACAAAGAAAAAAG+AGG - chr1.1:55455454-55455473 MS.gene004728:CDS 30.0%
TATGAGAAATATTCATGACC+AGG + chr1.1:55454509-55454528 None:intergenic 30.0%
TCAAACTTGCAAAGAAATCA+TGG - chr1.1:55454102-55454121 MS.gene004728:CDS 30.0%
TGTAAATCAATAACCCGAAT+TGG + chr1.1:55454457-55454476 None:intergenic 30.0%
! AGGGATTACTTGATGATAAA+TGG - chr1.1:55455563-55455582 MS.gene004728:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG - chr1.1:55455566-55455585 MS.gene004728:CDS 30.0%
! GTTGAAATTTTGGATGGTTT+AGG - chr1.1:55455292-55455311 MS.gene004728:CDS 30.0%
!! ATACTGGTTGAAATTTTGGA+TGG - chr1.1:55455286-55455305 MS.gene004728:CDS 30.0%
ACAAAGAAAAAAGAGGCAGA+AGG - chr1.1:55455461-55455480 MS.gene004728:CDS 35.0%
AGCAATTCTGTATGAGAAGA+AGG - chr1.1:55455361-55455380 MS.gene004728:CDS 35.0%
CAAAGAAAAAAGAGGCAGAA+GGG - chr1.1:55455462-55455481 MS.gene004728:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG - chr1.1:55454475-55454494 MS.gene004728:CDS 35.0%
GAAAGTTATTGCTATGCAGA+GGG - chr1.1:55454667-55454686 MS.gene004728:intron 35.0%
GATAGATTTCGACTTAGACT+TGG + chr1.1:55455237-55455256 None:intergenic 35.0%
TAAACTCTCGAATCATGTCA+AGG - chr1.1:55454304-55454323 MS.gene004728:CDS 35.0%
TAGTTCAAGGAATCATCGAA+GGG - chr1.1:55454330-55454349 MS.gene004728:CDS 35.0%
TCATGGACAGTCTTAAATAG+AGG + chr1.1:55455746-55455765 None:intergenic 35.0%
TGATTCGAGAGTTTATCATG+AGG + chr1.1:55454299-55454318 None:intergenic 35.0%
! AAGAACGAAAGGCAGTTTTT+CGG + chr1.1:55454204-55454223 None:intergenic 35.0%
! ATATTTGCATGGACTAACCT+TGG + chr1.1:55454920-55454939 None:intergenic 35.0%
! TAGTGTTGAGTATGAAACCA+AGG - chr1.1:55454900-55454919 MS.gene004728:intron 35.0%
! TTAAGAGCTTTTGTCGGATA+AGG + chr1.1:55455804-55455823 None:intergenic 35.0%
! TTTAGGGCAAAAGAAGCAAA+TGG - chr1.1:55454529-55454548 MS.gene004728:CDS 35.0%
AGTACACTTCTCAATGCACT+TGG - chr1.1:55455521-55455540 MS.gene004728:CDS 40.0%
AGTATTTGGACTAATGGAGG+TGG - chr1.1:55455650-55455669 MS.gene004728:CDS 40.0%
AGTTCAAGGAATCATCGAAG+GGG - chr1.1:55454331-55454350 MS.gene004728:CDS 40.0%
CATGTCAAGGAACTAGTTCA+AGG - chr1.1:55454317-55454336 MS.gene004728:CDS 40.0%
CGAAAGTTATTGCTATGCAG+AGG - chr1.1:55454666-55454685 MS.gene004728:intron 40.0%
CTAGTTCAAGGAATCATCGA+AGG - chr1.1:55454329-55454348 MS.gene004728:CDS 40.0%
GAATGCAGCAAAAATTGACG+CGG - chr1.1:55454640-55454659 MS.gene004728:intron 40.0%
GCTAAGATGAAAGGTGAAGT+TGG - chr1.1:55454587-55454606 MS.gene004728:intron 40.0%
TCAGTTTAGCGTATTTGAGG+AGG + chr1.1:55454271-55454290 None:intergenic 40.0%
TGCGAATGTTAAGCAATTGG+TGG - chr1.1:55454478-55454497 MS.gene004728:CDS 40.0%
TGGACAGTCTTAAATAGAGG+TGG + chr1.1:55455743-55455762 None:intergenic 40.0%
TTAGGTACAAGAGGCTAACT+GGG - chr1.1:55455310-55455329 MS.gene004728:CDS 40.0%
TTGGATGGTTTAGGTACAAG+AGG - chr1.1:55455301-55455320 MS.gene004728:CDS 40.0%
TTTAGGTACAAGAGGCTAAC+TGG - chr1.1:55455309-55455328 MS.gene004728:CDS 40.0%
! AAACGACTACACAGCTCTAA+GGG - chr1.1:55455544-55455563 MS.gene004728:CDS 40.0%
! AAGAAATCATGGGTTTTCCC+TGG - chr1.1:55454113-55454132 MS.gene004728:CDS 40.0%
! ACTTCGAAGGTATAGTTCAC+AGG + chr1.1:55454167-55454186 None:intergenic 40.0%
! GGAATAACGGAAGGATCTAT+GGG - chr1.1:55455683-55455702 MS.gene004728:CDS 40.0%
!! TGAAGTTGGCTCGAAATCTA+GGG - chr1.1:55454601-55454620 MS.gene004728:intron 40.0%
AAGACTGTCCATGAGCAAAC+AGG - chr1.1:55455752-55455771 MS.gene004728:CDS 45.0%
ACTCATGGCTTGAACTTCGA+AGG + chr1.1:55454180-55454199 None:intergenic 45.0%
AGAGTTGGCGAAGAAGAAAG+CGG - chr1.1:55454735-55454754 MS.gene004728:intron 45.0%
AGGACCAATTGTGAAGACAG+CGG + chr1.1:55454228-55454247 None:intergenic 45.0%
AGGATCTATGGGCATGAAAG+AGG - chr1.1:55455694-55455713 MS.gene004728:CDS 45.0%
AGGTATAGTTCACAGGTGAG+AGG + chr1.1:55454160-55454179 None:intergenic 45.0%
CAGAAGGGATTGTGACACTT+GGG - chr1.1:55455477-55455496 MS.gene004728:CDS 45.0%
GAAACGACTACACAGCTCTA+AGG - chr1.1:55455543-55455562 MS.gene004728:CDS 45.0%
GATGGCTAAGATCAATGCTG+AGG - chr1.1:55455601-55455620 MS.gene004728:CDS 45.0%
GTGGCAGAAGCTAAGATGAA+AGG - chr1.1:55454578-55454597 MS.gene004728:intron 45.0%
TCTGTATGAGAAGAAGGCAG+AGG - chr1.1:55455367-55455386 MS.gene004728:CDS 45.0%
TGGTGGCATGTTTCAAGAGA+TGG - chr1.1:55455583-55455602 MS.gene004728:CDS 45.0%
! ACCATGGAAGAGGTTTTCAG+AGG - chr1.1:55454380-55454399 MS.gene004728:CDS 45.0%
! CGGAATAACGGAAGGATCTA+TGG - chr1.1:55455682-55455701 MS.gene004728:CDS 45.0%
! TCCTCTGAAAACCTCTTCCA+TGG + chr1.1:55454384-55454403 None:intergenic 45.0%
!! GTGAAGTTGGCTCGAAATCT+AGG - chr1.1:55454600-55454619 MS.gene004728:intron 45.0%
!! TGCTAAAGCTGTGGCTCTAA+GGG - chr1.1:55454795-55454814 MS.gene004728:intron 45.0%
!!! TTATTTTTTAATATGTTATA+TGG - chr1.1:55455066-55455085 MS.gene004728:CDS 5.0%
AAGACAGCGGGAAGAACGAA+AGG + chr1.1:55454215-55454234 None:intergenic 50.0%
AAGACGGTGCAAGATTGACC+AGG + chr1.1:55454134-55454153 None:intergenic 50.0%
ACTAATGGAGGTGGCAATGG+AGG - chr1.1:55455659-55455678 MS.gene004728:CDS 50.0%
AGACGGTGCAAGATTGACCA+GGG + chr1.1:55454133-55454152 None:intergenic 50.0%
ATCAATGCTGAGGCAGTACG+TGG - chr1.1:55455611-55455630 MS.gene004728:CDS 50.0%
ATGGGCATGAAAGAGGTTGC+TGG - chr1.1:55455701-55455720 MS.gene004728:CDS 50.0%
ATGTTGCCTCCTGCTTGGAT+GGG - chr1.1:55455776-55455795 MS.gene004728:CDS 50.0%
CTAAGGGAAGCTGAGTTGCA+GGG - chr1.1:55454811-55454830 MS.gene004728:intron 50.0%
CTGGAGAGAGTGACAAAGAG+AGG - chr1.1:55454690-55454709 MS.gene004728:intron 50.0%
CTTCCCGCTGTCTTCACAAT+TGG - chr1.1:55454221-55454240 MS.gene004728:CDS 50.0%
CTTTCCTGATCGTCAACACG+AGG + chr1.1:55454248-55454267 None:intergenic 50.0%
GAATCAAGCTAGGGTTGACG+TGG - chr1.1:55454559-55454578 MS.gene004728:intron 50.0%
GATAAGGTTCCCATCCAAGC+AGG + chr1.1:55455788-55455807 None:intergenic 50.0%
GATGTCTTCGATTCCAGCTC+CGG + chr1.1:55454084-55454103 None:intergenic 50.0%
GCAACATGCCTGTTTGCTCA+TGG + chr1.1:55455763-55455782 None:intergenic 50.0%
GCAGAAGGGATTGTGACACT+TGG - chr1.1:55455476-55455495 MS.gene004728:CDS 50.0%
GGAAGCAGCGAATCAAGCTA+GGG - chr1.1:55454550-55454569 MS.gene004728:intron 50.0%
GGACCAATTGTGAAGACAGC+GGG + chr1.1:55454227-55454246 None:intergenic 50.0%
GGCTGAGGCTAATTCAGAGT+TGG - chr1.1:55454720-55454739 MS.gene004728:intron 50.0%
GTGACAAAGAGAGGCTGAAG+TGG - chr1.1:55454699-55454718 MS.gene004728:intron 50.0%
GTGACACTTGGGAATGCTCA+AGG - chr1.1:55455488-55455507 MS.gene004728:CDS 50.0%
GTTATTGCTATGCAGAGGGC+TGG - chr1.1:55454671-55454690 MS.gene004728:intron 50.0%
TCCGAGTACCTGGTGATAAC+CGG - chr1.1:55454062-55454081 MS.gene004728:CDS 50.0%
TCCGGTTATCACCAGGTACT+CGG + chr1.1:55454066-55454085 None:intergenic 50.0%
TCTAAGGGAAGCTGAGTTGC+AGG - chr1.1:55454810-55454829 MS.gene004728:intron 50.0%
TGCTTCGATGACCATGGAAG+AGG - chr1.1:55454370-55454389 MS.gene004728:CDS 50.0%
TGGAAGCAGCGAATCAAGCT+AGG - chr1.1:55454549-55454568 MS.gene004728:intron 50.0%
TGGACTAATGGAGGTGGCAA+TGG - chr1.1:55455656-55455675 MS.gene004728:CDS 50.0%
TGGCAATGGAGGCGGAATAA+CGG - chr1.1:55455670-55455689 MS.gene004728:CDS 50.0%
! AAGCAATTGGTGGATGTGCC+TGG - chr1.1:55454488-55454507 MS.gene004728:CDS 50.0%
! AATGGAGGCGGAATAACGGA+AGG - chr1.1:55455674-55455693 MS.gene004728:CDS 50.0%
! CTGCTAAAGCTGTGGCTCTA+AGG - chr1.1:55454794-55454813 MS.gene004728:intron 50.0%
!! AGCAAAAGCATCCGAGTACC+TGG - chr1.1:55454052-55454071 MS.gene004728:CDS 50.0%
!! AGGCAGTTTTTCGGCACTCA+TGG + chr1.1:55454195-55454214 None:intergenic 50.0%
!! GTTGGCTCGAAATCTAGGGA+AGG - chr1.1:55454605-55454624 MS.gene004728:intron 50.0%
AAGAGAGGCTGAAGTGGCTG+AGG - chr1.1:55454705-55454724 MS.gene004728:intron 55.0%
AAGGTTCCCATCCAAGCAGG+AGG + chr1.1:55455785-55455804 None:intergenic 55.0%
AATGGAGGTGGCAATGGAGG+CGG - chr1.1:55455662-55455681 MS.gene004728:CDS 55.0%
AGTCGAGGCTGCTAAAGCTG+TGG - chr1.1:55454786-55454805 MS.gene004728:intron 55.0%
AGTTGCAGGGTGAGGTTGAG+AGG - chr1.1:55454824-55454843 MS.gene004728:intron 55.0%
CACAGGTGAGAGGTCGAAGA+CGG + chr1.1:55454150-55454169 None:intergenic 55.0%
CATGTTGCCTCCTGCTTGGA+TGG - chr1.1:55455775-55455794 MS.gene004728:CDS 55.0%
TACCTGGTGATAACCGGAGC+TGG - chr1.1:55454068-55454087 MS.gene004728:CDS 55.0%
TTCCAGCTCCGGTTATCACC+AGG + chr1.1:55454073-55454092 None:intergenic 55.0%
! GAAGAAAGCGGCGTGGACTA+AGG - chr1.1:55454747-55454766 MS.gene004728:intron 55.0%
! TGGTCCTCGTGTTGACGATC+AGG - chr1.1:55454241-55454260 MS.gene004728:CDS 55.0%
CAGGCATGTTGCCTCCTGCT+TGG - chr1.1:55455771-55455790 MS.gene004728:CDS 60.0%
CCATGGTCATCGAAGCAGCG+AGG + chr1.1:55454367-55454386 None:intergenic 60.0%
CCTCGCTGCTTCGATGACCA+TGG - chr1.1:55454364-55454383 MS.gene004728:CDS 60.0%
GGAAGCTGAGTTGCAGGGTG+AGG - chr1.1:55454816-55454835 MS.gene004728:intron 60.0%
GGCTCAAGTGGCTGAAGTCG+AGG - chr1.1:55454771-55454790 MS.gene004728:intron 60.0%
TGGCGAAGAAGAAAGCGGCG+TGG - chr1.1:55454740-55454759 MS.gene004728:intron 60.0%
! GAAAGCGGCGTGGACTAAGG+CGG - chr1.1:55454750-55454769 MS.gene004728:intron 60.0%
! GTGGACTAAGGCGGCTCAAG+TGG - chr1.1:55454759-55454778 MS.gene004728:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 55454041 55455823 55454041 ID=MS.gene004728
chr1.1 mRNA 55454041 55455823 55454041 ID=MS.gene004728.t1;Parent=MS.gene004728
chr1.1 exon 55455166 55455823 55455166 ID=MS.gene004728.t1.exon1;Parent=MS.gene004728.t1
chr1.1 CDS 55455166 55455823 55455166 ID=cds.MS.gene004728.t1;Parent=MS.gene004728.t1
chr1.1 exon 55454943 55455127 55454943 ID=MS.gene004728.t1.exon2;Parent=MS.gene004728.t1
chr1.1 CDS 55454943 55455127 55454943 ID=cds.MS.gene004728.t1;Parent=MS.gene004728.t1
chr1.1 exon 55454041 55454550 55454041 ID=MS.gene004728.t1.exon3;Parent=MS.gene004728.t1
chr1.1 CDS 55454041 55454550 55454041 ID=cds.MS.gene004728.t1;Parent=MS.gene004728.t1
Gene Sequence

>MS.gene004728

ATGTACAGGGTAGCAAAAGCATCCGAGTACCTGGTGATAACCGGAGCTGGAATCGAAGACATCAAACTTGCAAAGAAATCATGGGTTTTCCCTGGTCAATCTTGCACCGTCTTCGACCTCTCACCTGTGAACTATACCTTCGAAGTTCAAGCCATGAGTGCCGAAAAACTGCCTTTCGTTCTTCCCGCTGTCTTCACAATTGGTCCTCGTGTTGACGATCAGGAAAGCCTCCTCAAATACGCTAAACTGATTTCACCTCATGATAAACTCTCGAATCATGTCAAGGAACTAGTTCAAGGAATCATCGAAGGGGAGACACGTGTCCTCGCTGCTTCGATGACCATGGAAGAGGTTTTCAGAGGAACAAAAGAGTTTAAACAAGAAGTATTCGAGAAAGTTCAACTTGAACTAAACCAATTCGGGTTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCCTGGTCATGAATATTTCTCATATTTAGGGCAAAAGAAGCAAATGGAAGCAGCGAATCAAGCTAGGGTTGACGTGGCAGAAGCTAAGATGAAAGGTGAAGTTGGCTCGAAATCTAGGGAAGGACAAACACTGCAGAATGCAGCAAAAATTGACGCGGAGACGAAAGTTATTGCTATGCAGAGGGCTGGAGAGAGTGACAAAGAGAGGCTGAAGTGGCTGAGGCTAATTCAGAGTTGGCGAAGAAGAAAGCGGCGTGGACTAAGGCGGCTCAAGTGGCTGAAGTCGAGGCTGCTAAAGCTGTGGCTCTAAGGGAAGCTGAGTTGCAGGGTGAGGTTGAGAGGATGAATGCTTTGACTACTACTCAGAAGCTTAAAGCTGATTTACTTAGCAAAGCTAGTGTTGAGTATGAAACCAAGGTTAGTCCATGCAAATATAACTCTTCTTTCATTTATTAATTATTATTATTATTATTCAAATTGTTTTACTAGCAACAATATTAATACTTCAATATCTTTTGTTAGAAAATAAGTCACTGTAGTTAACGAGACTTATTTTTTATTTTATTTTTTAATATGTTATATGGATTATGGAATAGTCAATTTTTTTTGCATACATGAATCTATCATTTGCTAATTTTATGTATCATTATAAAACTAAATCATATGGTATGTTCATATAATATTTGTTTCATGACAATTTTGTTCTAAATTAATTTTGTTAGGAATGTGCCAAGTCTAAGTCGAAATCTATCATGAATGATTTTCAATGTTTATTTTTTAAATACTGGTTGAAATTTTGGATGGTTTAGGTACAAGAGGCTAACTGGGAATTATACAAGAAACAAAAAGAAGCAGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATTGTCAGATGCAACATTTTATGCGCGCAAGCAAGCAGCTGAAGCAGAGCTATACACAAAGAAAAAAGAGGCAGAAGGGATTGTGACACTTGGGAATGCTCAAGGAGTGTATGTAAGTACACTTCTCAATGCACTTGGAAACGACTACACAGCTCTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATGGCTAAGATCAATGCTGAGGCAGTACGTGGACTTGAACCAAAGATTAGTATTTGGACTAATGGAGGTGGCAATGGAGGCGGAATAACGGAAGGATCTATGGGCATGAAAGAGGTTGCTGGTGTTTACAAGATGCTGCCACCTCTATTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCTGCTTGGATGGGAACCTTATCCGACAAAAGCTCTTAA
Protein sequence

>MS.gene004728.t1

MYRVAKASEYLVITGAGIEDIKLAKKSWVFPGQSCTVFDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDKLSNHVKELVQGIIEGETRVLAASMTMEEVFRGTKEFKQEVFEKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKKQMEAANQARVDVAEAKMKGEVGSKSREGQTLQNAAKIDAETKVIAMQRAGEKLAKKKAAWTKAAQVAEVEAAKAVALREAELQGEVERMNALTTTQKLKADLLSKASVEYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKALSDATFYARKQAAEAELYTKKKEAEGIVTLGNAQGVYVSTLLNALGNDYTALRDYLMINGGMFQEMAKINAEAVRGLEPKISIWTNGGGNGGGITEGSMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGTLSDKSS