AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62244

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62244.t1 MTR_3g106420 95.625 480 18 1 1 477 1 480 0.0 929
MS.gene62244.t1 MTR_3g106480 91.423 478 38 1 3 477 1 478 0.0 889
MS.gene62244.t1 MTR_3g106485 90.795 478 37 3 3 477 1 474 0.0 871
MS.gene62244.t1 MTR_1g083190 90.417 480 35 2 1 477 1 472 0.0 865
MS.gene62244.t1 MTR_1g115145 87.056 479 52 4 3 477 1 473 0.0 828
MS.gene62244.t1 MTR_1g073400 87.238 478 54 2 3 477 1 474 0.0 827
MS.gene62244.t1 MTR_3g106430 88.470 477 48 3 3 476 1 473 0.0 823
MS.gene62244.t1 MTR_1g071130 85.263 475 44 2 3 477 1 449 0.0 822
MS.gene62244.t1 MTR_3g106470 80.084 477 36 3 3 476 32 452 0.0 745
MS.gene62244.t1 MTR_8g063830 79.355 465 52 6 3 463 1 425 0.0 671
MS.gene62244.t1 MTR_3g065540 63.478 115 15 1 42 156 14 101 2.46e-39 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62244.t1 AT5G25260 73.629 474 110 3 3 473 1 462 0.0 719
MS.gene62244.t1 AT5G25250 73.573 473 108 3 3 472 1 459 0.0 706
MS.gene62244.t1 AT5G64870 70.339 472 126 2 4 472 3 463 0.0 647

Find 118 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGAGTATTTCTCATATT+TGG 0.110239 3.2:+62053321 MS.gene62244:CDS
TTGAGCCTAAGATTAGTATT+TGG 0.251063 3.2:+62054528 MS.gene62244:CDS
CATGAGTATTTCTCATATTT+GGG 0.292202 3.2:+62053322 MS.gene62244:CDS
AGGGATTACTTGATGATAAA+TGG 0.297566 3.2:+62054455 MS.gene62244:CDS
CGAAGAAGGCTGTGGCTCTA+AGG 0.317541 3.2:+62053641 MS.gene62244:CDS
GTCAACCCTAGCTTGATTCT+TGG 0.318930 3.2:-62053365 None:intergenic
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 3.2:+62053397 MS.gene62244:CDS
CATCAAGAATGAAGATTTAC+CGG 0.319614 3.2:+62052837 None:intergenic
AACAAGTTCATTAACGTGAT+TGG 0.321473 3.2:-62053122 None:intergenic
CGCCTCAACTTCAGCCGCCT+TGG 0.340207 3.2:-62053620 None:intergenic
CAACTTGAGTTGAATCAATT+CGG 0.350972 3.2:+62053250 MS.gene62244:CDS
GTGAAATTGGTTCAAAATTG+AGG 0.384625 3.2:+62053410 MS.gene62244:CDS
TTAGTCCAAATACTAATCTT+AGG 0.384650 3.2:-62054533 None:intergenic
AAGATTAGTATTTGGACTAA+TGG 0.384982 3.2:+62054536 MS.gene62244:CDS
ACTTTGAAGGTGTAGTTTAC+CGG 0.385250 3.2:-62052974 None:intergenic
CAATGCGAATGTTAAGCAAT+TGG 0.388576 3.2:+62053285 MS.gene62244:CDS
CAAGACGGTGCAGGATTGAC+TGG 0.396621 3.2:-62052942 None:intergenic
TCAGAATACCTTGTGATAAC+AGG 0.405430 3.2:+62052872 MS.gene62244:CDS
TGTAGGTACAAGAAGCAAAC+TGG 0.406248 3.2:+62054201 MS.gene62244:intron
TGGTCCTCGTGTTGACGATC+AGG 0.407219 3.2:+62053051 MS.gene62244:CDS
TGGACTAATGGTGGTGATAA+TGG 0.423773 3.2:+62054548 MS.gene62244:CDS
CACGTTAATGAACTTGTTCA+AGG 0.425303 3.2:+62053127 MS.gene62244:CDS
AGCTGGAGAGGGTGAGAAGG+AGG 0.426976 3.2:+62053498 MS.gene62244:CDS
CATGTTGCCTCCTGCATGGA+TGG 0.431761 3.2:+62054667 MS.gene62244:CDS
TGAAATTGGTTCAAAATTGA+GGG 0.433449 3.2:+62053411 MS.gene62244:CDS
CTCCCCGCTGTCTTCACCAT+TGG 0.433479 3.2:+62053031 MS.gene62244:CDS
ATCATGTGGTGAAATAAGTT+TGG 0.438830 3.2:-62053092 None:intergenic
CTTTGCATATAGCTCTGCTT+CGG 0.455229 3.2:-62054336 None:intergenic
TAATGGTGGTGATAATGGTG+GGG 0.455818 3.2:+62054553 MS.gene62244:CDS
GGCTGAAGTTGAGGCGAAGA+AGG 0.456977 3.2:+62053627 MS.gene62244:CDS
ACTCATAGCTTGAACTTTGA+AGG 0.458699 3.2:-62052987 None:intergenic
GCAGAGGGAACCGTGACCCT+TGG 0.465294 3.2:+62054368 MS.gene62244:CDS
CAGAGGGAACCGTGACCCTT+GGG 0.472084 3.2:+62054369 MS.gene62244:CDS
CAGTCAATCCTGCACCGTCT+TGG 0.482767 3.2:+62052943 MS.gene62244:CDS
GGACCAATGGTGAAGACAGC+GGG 0.486586 3.2:-62053034 None:intergenic
AAGACTGTCCATGAGCAAAC+AGG 0.492575 3.2:+62054644 MS.gene62244:CDS
ATTCTGATGCTTTCGCGACC+CGG 0.494061 3.2:-62052856 None:intergenic
GAGAGCTGGAGAGGGTGAGA+AGG 0.498051 3.2:+62053495 MS.gene62244:CDS
CTCAAGTCCGCGTATCGCCT+CGG 0.498347 3.2:-62054510 None:intergenic
AAGACAGCGGGGAGAACAAA+AGG 0.503055 3.2:-62053022 None:intergenic
TATCATCAAGTAATCCCTTA+CGG 0.507171 3.2:-62054450 None:intergenic
GCTGGAGAGGGTGAGAAGGA+GGG 0.508003 3.2:+62053499 MS.gene62244:CDS
GTTATTGCGATGCAGAGAGC+TGG 0.520901 3.2:+62053481 MS.gene62244:CDS
GCAACATGCCTGTTTGCTCA+TGG 0.521020 3.2:-62054652 None:intergenic
AGCAATTCTGTACGAGAAGA+AGG 0.521753 3.2:+62054253 MS.gene62244:CDS
GAGAACAAAAGGAAGTTTCT+CGG 0.527868 3.2:-62053011 None:intergenic
ATTAGTATTTGGACTAATGG+TGG 0.532835 3.2:+62054539 MS.gene62244:CDS
TAAGTTTGGCGTATTTGAGA+AGG 0.534048 3.2:-62053078 None:intergenic
TGAGAGGTCCAAGACGGTGC+AGG 0.535402 3.2:-62052951 None:intergenic
GACCAAGGCGGCTGAAGTTG+AGG 0.538230 3.2:+62053618 MS.gene62244:CDS
CACCGTCTTGGACCTCTCAC+CGG 0.539796 3.2:+62052955 MS.gene62244:CDS
TTGAGCATTCCCAAGGGTCA+CGG 0.540006 3.2:-62054378 None:intergenic
GGCTCAAGCTAATTCTGAGT+TGG 0.541784 3.2:+62053576 MS.gene62244:CDS
AATGGTGGTGATAATGGTGG+GGG 0.542270 3.2:+62054554 MS.gene62244:CDS
GAAGAAGGCTGTGGCTCTAA+GGG 0.545005 3.2:+62053642 MS.gene62244:CDS
TGGAAGCCAAGAATCAAGCT+AGG 0.548842 3.2:+62053359 MS.gene62244:CDS
ATATGAGAAATACTCATGAC+CGG 0.550689 3.2:-62053317 None:intergenic
GATAAGGATCCCATCCATGC+AGG 0.550974 3.2:-62054677 None:intergenic
AAGCAATTGGTGGATGTGCC+CGG 0.551180 3.2:+62053298 MS.gene62244:CDS
GGGATAACTGAAGGTGACAT+TGG 0.554586 3.2:+62054575 MS.gene62244:CDS
TAAAGTGAGGACTGAAGTGA+AGG 0.556664 3.2:+62053525 MS.gene62244:CDS
AGTTGAGGCGAAGAAGGCTG+TGG 0.558891 3.2:+62053633 MS.gene62244:CDS
TGCGAATGTTAAGCAATTGG+TGG 0.563120 3.2:+62053288 MS.gene62244:CDS
ATTGAACACTAGCTTTGCTA+AGG 0.566032 3.2:-62053735 None:intergenic
GAAACAACTACACAGCCGTA+AGG 0.567841 3.2:+62054435 MS.gene62244:CDS
AGTACACTTCTCAATGCACT+TGG 0.570595 3.2:+62054413 MS.gene62244:CDS
GAAAACTTCCTCCATCGTCA+TGG 0.571023 3.2:-62053185 None:intergenic
ATGTTGCCTCCTGCATGGAT+GGG 0.571620 3.2:+62054668 MS.gene62244:CDS
GAAGAAAGCGGCTTGGACCA+AGG 0.572319 3.2:+62053603 MS.gene62244:CDS
ATTGGTTCAAAATTGAGGGA+AGG 0.580771 3.2:+62053415 MS.gene62244:CDS
TGCGATGCAGAGAGCTGGAG+AGG 0.580805 3.2:+62053486 MS.gene62244:CDS
ATCGTCAACACGAGGACCAA+TGG 0.584843 3.2:-62053047 None:intergenic
TGGCTAAGAAGAAAGCGGCT+TGG 0.585206 3.2:+62053596 MS.gene62244:CDS
CTTGTTCAAGGTATCATCGA+AGG 0.588052 3.2:+62053139 MS.gene62244:CDS
ATTGGTATGAAAGAAGTTGC+TGG 0.588411 3.2:+62054593 MS.gene62244:CDS
ACGCTCCTTGAGCATTCCCA+AGG 0.588853 3.2:-62054385 None:intergenic
GTGACCCTTGGGAATGCTCA+AGG 0.591886 3.2:+62054380 MS.gene62244:CDS
GAATCAAAGAGAGGATGAAG+TGG 0.593587 3.2:+62053555 MS.gene62244:CDS
CTAATGGTGGTGATAATGGT+GGG 0.595336 3.2:+62054552 MS.gene62244:CDS
AAGACGGTGCAGGATTGACT+GGG 0.597395 3.2:-62052941 None:intergenic
TTGTTCAAGGTATCATCGAA+GGG 0.598521 3.2:+62053140 MS.gene62244:CDS
TCTGTACGAGAAGAAGGCAG+AGG 0.600808 3.2:+62054259 MS.gene62244:CDS
GCGATGCAGAGAGCTGGAGA+GGG 0.601209 3.2:+62053487 MS.gene62244:CDS
AGGTGTAGTTTACCGGTGAG+AGG 0.602217 3.2:-62052967 None:intergenic
AGAAGGAGGGAATTAAAGTG+AGG 0.607130 3.2:+62053512 MS.gene62244:CDS
ACTAATGGTGGTGATAATGG+TGG 0.611329 3.2:+62054551 MS.gene62244:CDS
CAGGCATGTTGCCTCCTGCA+TGG 0.611578 3.2:+62054663 MS.gene62244:CDS
ATCAAGAATGAAGATTTACC+GGG 0.612301 3.2:+62052838 None:intergenic
CTAAGGGAAGCTGAGTTGCA+AGG 0.613783 3.2:+62053658 MS.gene62244:CDS
TTTGGGTCAAAAGACGCAGA+TGG 0.613836 3.2:+62053339 MS.gene62244:CDS
GTGTCAGAAGCTAAGATGAA+AGG 0.614706 3.2:+62053388 MS.gene62244:CDS
GTAGGTACAAGAAGCAAACT+GGG 0.614898 3.2:+62054202 MS.gene62244:intron
TCATGGACAGTCTTAAACAA+AGG 0.619848 3.2:-62054635 None:intergenic
TGCTGCTTCCATGACGATGG+AGG 0.622104 3.2:+62053177 MS.gene62244:CDS
AAACAACTACACAGCCGTAA+GGG 0.623742 3.2:+62054436 MS.gene62244:CDS
CGCTCCTTGAGCATTCCCAA+GGG 0.624114 3.2:-62054384 None:intergenic
ATTAATGCCGAGGCGATACG+CGG 0.629875 3.2:+62054503 MS.gene62244:CDS
TGAGTTGGCTAAGAAGAAAG+CGG 0.631786 3.2:+62053591 MS.gene62244:CDS
GGAAGCCAAGAATCAAGCTA+GGG 0.640292 3.2:+62053360 MS.gene62244:CDS
GATTGCTAAGATTAATGCCG+AGG 0.643593 3.2:+62054493 MS.gene62244:CDS
GCTGTACAAGAAACAGAAAG+AGG 0.643966 3.2:+62054226 MS.gene62244:CDS
TATGAGAAATACTCATGACC+GGG 0.646479 3.2:-62053316 None:intergenic
ACTTGCTGCTTCCATGACGA+TGG 0.648427 3.2:+62053174 MS.gene62244:CDS
TGGACAGTCTTAAACAAAGG+TGG 0.649112 3.2:-62054632 None:intergenic
TAGTGTTCAATATGAAACCA+AGG 0.651992 3.2:+62053747 MS.gene62244:CDS
GAAAATTGATGCAGAGACGA+AGG 0.652587 3.2:+62053459 MS.gene62244:CDS
AGTTGCAAGGTGAAGTTGAG+AGG 0.653383 3.2:+62053671 MS.gene62244:CDS
GATTACTTGATGATAAATGG+TGG 0.658453 3.2:+62054458 MS.gene62244:CDS
TACCGGTGAGAGGTCCAAGA+CGG 0.667806 3.2:-62052957 None:intergenic
AAGGATCCCATCCATGCAGG+AGG 0.669522 3.2:-62054674 None:intergenic
TGAAAATACCTGTTATCACA+AGG 0.669974 3.2:-62052880 None:intergenic
AGGACCAATGGTGAAGACAG+CGG 0.683272 3.2:-62053035 None:intergenic
CTTTCCTGATCGTCAACACG+AGG 0.689163 3.2:-62053055 None:intergenic
TGTTCAAGGTATCATCGAAG+GGG 0.695602 3.2:+62053141 MS.gene62244:CDS
TGATTGGAGTGTCGATCATG+TGG 0.697521 3.2:-62053106 None:intergenic
GACCAATGGTGAAGACAGCG+GGG 0.714278 3.2:-62053033 None:intergenic
ATGGTGGTGATAATGGTGGG+GGG 0.731868 3.2:+62054555 MS.gene62244:CDS
GAAAGCGGCTTGGACCAAGG+CGG 0.735762 3.2:+62053606 MS.gene62244:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATATATATATTATAT+AGG + chr3.2:62053841-62053860 MS.gene62244:intron 0.0%
!!! ATTTTCAACATTTACTTTTT+AGG + chr3.2:62054118-62054137 MS.gene62244:intron 15.0%
!!! TTTTTTGGTGATTAAAATTT+TGG + chr3.2:62054174-62054193 MS.gene62244:intron 15.0%
!! ATATAGGAGATTATTCAATA+TGG + chr3.2:62053857-62053876 MS.gene62244:intron 20.0%
!!! TCTTATATAGAACACTTTTA+TGG - chr3.2:62054092-62054111 None:intergenic 20.0%
!!! TTTACTGGATTTTACTTTTT+TGG + chr3.2:62054159-62054178 MS.gene62244:intron 20.0%
! AAGATTAGTATTTGGACTAA+TGG + chr3.2:62054536-62054555 MS.gene62244:CDS 25.0%
! CATGAGTATTTCTCATATTT+GGG + chr3.2:62053322-62053341 MS.gene62244:CDS 25.0%
! TCATGAGTATTTCTCATATT+TGG + chr3.2:62053321-62053340 MS.gene62244:CDS 25.0%
! TGAAAGAAGTTATATTTGCA+TGG - chr3.2:62053783-62053802 None:intergenic 25.0%
! TTAGTCCAAATACTAATCTT+AGG - chr3.2:62054536-62054555 None:intergenic 25.0%
!! ATTAAAATTTTGGATGGTGT+AGG + chr3.2:62054184-62054203 MS.gene62244:intron 25.0%
!! TGAAATTGGTTCAAAATTGA+GGG + chr3.2:62053411-62053430 MS.gene62244:CDS 25.0%
!!! AACATTTACTTTTTAGGTGT+TGG + chr3.2:62054124-62054143 MS.gene62244:intron 25.0%
!!! TGGCTATTTGATAATTTTAC+TGG + chr3.2:62054144-62054163 MS.gene62244:intron 25.0%
!!! TTGGTGATTAAAATTTTGGA+TGG + chr3.2:62054178-62054197 MS.gene62244:intron 25.0%
AACAAGTTCATTAACGTGAT+TGG - chr3.2:62053125-62053144 None:intergenic 30.0%
ACAAGACAAAACAATTCAAG+TGG - chr3.2:62054067-62054086 None:intergenic 30.0%
ATATGAGAAATACTCATGAC+CGG - chr3.2:62053320-62053339 None:intergenic 30.0%
ATCATGTGGTGAAATAAGTT+TGG - chr3.2:62053095-62053114 None:intergenic 30.0%
ATTAGTATTTGGACTAATGG+TGG + chr3.2:62054539-62054558 MS.gene62244:CDS 30.0%
GCTATATGCAAAGAAAAAAG+AGG + chr3.2:62054346-62054365 MS.gene62244:CDS 30.0%
TAGTGTTCAATATGAAACCA+AGG + chr3.2:62053747-62053766 MS.gene62244:CDS 30.0%
TATCATCAAGTAATCCCTTA+CGG - chr3.2:62054453-62054472 None:intergenic 30.0%
TCAAACTCAAAAAGAAAGCA+TGG + chr3.2:62052912-62052931 MS.gene62244:CDS 30.0%
TGAAAATACCTGTTATCACA+AGG - chr3.2:62052883-62052902 None:intergenic 30.0%
TTGAGCCTAAGATTAGTATT+TGG + chr3.2:62054528-62054547 MS.gene62244:CDS 30.0%
! AGGGATTACTTGATGATAAA+TGG + chr3.2:62054455-62054474 MS.gene62244:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG + chr3.2:62054458-62054477 MS.gene62244:CDS 30.0%
! GTGAAATTGGTTCAAAATTG+AGG + chr3.2:62053410-62053429 MS.gene62244:CDS 30.0%
! TTAAGAGCTTTTGTCAGATA+AGG - chr3.2:62054696-62054715 None:intergenic 30.0%
!! CAACTTGAGTTGAATCAATT+CGG + chr3.2:62053250-62053269 MS.gene62244:CDS 30.0%
ACTCATAGCTTGAACTTTGA+AGG - chr3.2:62052990-62053009 None:intergenic 35.0%
ATTGGTATGAAAGAAGTTGC+TGG + chr3.2:62054593-62054612 MS.gene62244:CDS 35.0%
CAATGCGAATGTTAAGCAAT+TGG + chr3.2:62053285-62053304 MS.gene62244:CDS 35.0%
GAGAACAAAAGGAAGTTTCT+CGG - chr3.2:62053014-62053033 None:intergenic 35.0%
GATGAGAACAATGTCTGTTT+GGG + chr3.2:62053902-62053921 MS.gene62244:intron 35.0%
GCTAAGATGAAAGGTGAAAT+TGG + chr3.2:62053397-62053416 MS.gene62244:CDS 35.0%
TAAGTTTGGCGTATTTGAGA+AGG - chr3.2:62053081-62053100 None:intergenic 35.0%
TATGAGAAATACTCATGACC+GGG - chr3.2:62053319-62053338 None:intergenic 35.0%
TCAGAATACCTTGTGATAAC+AGG + chr3.2:62052872-62052891 MS.gene62244:CDS 35.0%
TCATGGACAGTCTTAAACAA+AGG - chr3.2:62054638-62054657 None:intergenic 35.0%
TGATGAGAACAATGTCTGTT+TGG + chr3.2:62053901-62053920 MS.gene62244:intron 35.0%
! ACTTTGAAGGTGTAGTTTAC+CGG - chr3.2:62052977-62052996 None:intergenic 35.0%
! ATTGGTTCAAAATTGAGGGA+AGG + chr3.2:62053415-62053434 MS.gene62244:CDS 35.0%
! CACGTTAATGAACTTGTTCA+AGG + chr3.2:62053127-62053146 MS.gene62244:CDS 35.0%
! TTGTTCAAGGTATCATCGAA+GGG + chr3.2:62053140-62053159 MS.gene62244:CDS 35.0%
!! ATTGAACACTAGCTTTGCTA+AGG - chr3.2:62053738-62053757 None:intergenic 35.0%
!! GGTTTTTGAGAATCAAAGAG+AGG + chr3.2:62053546-62053565 MS.gene62244:CDS 35.0%
AAACAACTACACAGCCGTAA+GGG + chr3.2:62054436-62054455 MS.gene62244:CDS 40.0%
AGAAGGAGGGAATTAAAGTG+AGG + chr3.2:62053512-62053531 MS.gene62244:CDS 40.0%
AGCAATTCTGTACGAGAAGA+AGG + chr3.2:62054253-62054272 MS.gene62244:CDS 40.0%
AGTACACTTCTCAATGCACT+TGG + chr3.2:62054413-62054432 MS.gene62244:CDS 40.0%
CAATGTCTGTTTGGGTGTTT+AGG + chr3.2:62053910-62053929 MS.gene62244:intron 40.0%
GAAAATTGATGCAGAGACGA+AGG + chr3.2:62053459-62053478 MS.gene62244:CDS 40.0%
GAATCAAAGAGAGGATGAAG+TGG + chr3.2:62053555-62053574 MS.gene62244:CDS 40.0%
GATTGCTAAGATTAATGCCG+AGG + chr3.2:62054493-62054512 MS.gene62244:CDS 40.0%
GCAAAGAAAAAAGAGGCAGA+GGG + chr3.2:62054353-62054372 MS.gene62244:CDS 40.0%
GCTGTACAAGAAACAGAAAG+AGG + chr3.2:62054226-62054245 MS.gene62244:CDS 40.0%
GTAGGTACAAGAAGCAAACT+GGG + chr3.2:62054202-62054221 MS.gene62244:intron 40.0%
GTGTCAGAAGCTAAGATGAA+AGG + chr3.2:62053388-62053407 MS.gene62244:CDS 40.0%
TAAAGTGAGGACTGAAGTGA+AGG + chr3.2:62053525-62053544 MS.gene62244:CDS 40.0%
TGCAAAGAAAAAAGAGGCAG+AGG + chr3.2:62054352-62054371 MS.gene62244:CDS 40.0%
TGCGAATGTTAAGCAATTGG+TGG + chr3.2:62053288-62053307 MS.gene62244:CDS 40.0%
TGGACAGTCTTAAACAAAGG+TGG - chr3.2:62054635-62054654 None:intergenic 40.0%
TGTAGGTACAAGAAGCAAAC+TGG + chr3.2:62054201-62054220 MS.gene62244:intron 40.0%
! CTTGTTCAAGGTATCATCGA+AGG + chr3.2:62053139-62053158 MS.gene62244:CDS 40.0%
! CTTTGCATATAGCTCTGCTT+CGG - chr3.2:62054339-62054358 None:intergenic 40.0%
! TGAGTTGGCTAAGAAGAAAG+CGG + chr3.2:62053591-62053610 MS.gene62244:CDS 40.0%
! TGCATGGACTAAACTAACCT+TGG - chr3.2:62053767-62053786 None:intergenic 40.0%
! TGGACTAATGGTGGTGATAA+TGG + chr3.2:62054548-62054567 MS.gene62244:CDS 40.0%
! TGTTCAAGGTATCATCGAAG+GGG + chr3.2:62053141-62053160 MS.gene62244:CDS 40.0%
!! ACTAATGGTGGTGATAATGG+TGG + chr3.2:62054551-62054570 MS.gene62244:CDS 40.0%
!! CTAATGGTGGTGATAATGGT+GGG + chr3.2:62054552-62054571 MS.gene62244:CDS 40.0%
!! TAATGGTGGTGATAATGGTG+GGG + chr3.2:62054553-62054572 MS.gene62244:CDS 40.0%
AAGACTGTCCATGAGCAAAC+AGG + chr3.2:62054644-62054663 MS.gene62244:CDS 45.0%
AATGCTTAAGCAATGCCCCA+AGG - chr3.2:62054007-62054026 None:intergenic 45.0%
ACCAACATAGTCACGCTCAA+TGG + chr3.2:62053967-62053986 MS.gene62244:intron 45.0%
AGTTGCAAGGTGAAGTTGAG+AGG + chr3.2:62053671-62053690 MS.gene62244:CDS 45.0%
ATGCTTAAGCAATGCCCCAA+GGG - chr3.2:62054006-62054025 None:intergenic 45.0%
CCAACATAGTCACGCTCAAT+GGG + chr3.2:62053968-62053987 MS.gene62244:intron 45.0%
GAAAACTTCCTCCATCGTCA+TGG - chr3.2:62053188-62053207 None:intergenic 45.0%
GAAACAACTACACAGCCGTA+AGG + chr3.2:62054435-62054454 MS.gene62244:CDS 45.0%
GGAAGCCAAGAATCAAGCTA+GGG + chr3.2:62053360-62053379 MS.gene62244:CDS 45.0%
GGCTCAAGCTAATTCTGAGT+TGG + chr3.2:62053576-62053595 MS.gene62244:CDS 45.0%
GGGATAACTGAAGGTGACAT+TGG + chr3.2:62054575-62054594 MS.gene62244:CDS 45.0%
GTCAACCCTAGCTTGATTCT+TGG - chr3.2:62053368-62053387 None:intergenic 45.0%
TGGAAGCCAAGAATCAAGCT+AGG + chr3.2:62053359-62053378 MS.gene62244:CDS 45.0%
TTTGGGTCAAAAGACGCAGA+TGG + chr3.2:62053339-62053358 MS.gene62244:CDS 45.0%
! ACGATGGAGGAAGTTTTCAG+AGG + chr3.2:62053190-62053209 MS.gene62244:CDS 45.0%
!! AATGGTGGTGATAATGGTGG+GGG + chr3.2:62054554-62054573 MS.gene62244:CDS 45.0%
!! TGATTGGAGTGTCGATCATG+TGG - chr3.2:62053109-62053128 None:intergenic 45.0%
AAGACAGCGGGGAGAACAAA+AGG - chr3.2:62053025-62053044 None:intergenic 50.0%
AAGACGGTGCAGGATTGACT+GGG - chr3.2:62052944-62052963 None:intergenic 50.0%
ACATAGTCACGCTCAATGGG+TGG + chr3.2:62053971-62053990 MS.gene62244:intron 50.0%
AGGACCAATGGTGAAGACAG+CGG - chr3.2:62053038-62053057 None:intergenic 50.0%
AGGTGTAGTTTACCGGTGAG+AGG - chr3.2:62052970-62052989 None:intergenic 50.0%
ATCGTCAACACGAGGACCAA+TGG - chr3.2:62053050-62053069 None:intergenic 50.0%
ATGTTGCCTCCTGCATGGAT+GGG + chr3.2:62054668-62054687 MS.gene62244:CDS 50.0%
ATTAATGCCGAGGCGATACG+CGG + chr3.2:62054503-62054522 MS.gene62244:CDS 50.0%
ATTCTGATGCTTTCGCGACC+CGG - chr3.2:62052859-62052878 None:intergenic 50.0%
CCCATTGAGCGTGACTATGT+TGG - chr3.2:62053971-62053990 None:intergenic 50.0%
CTAAGGGAAGCTGAGTTGCA+AGG + chr3.2:62053658-62053677 MS.gene62244:CDS 50.0%
CTTTCCTGATCGTCAACACG+AGG - chr3.2:62053058-62053077 None:intergenic 50.0%
GATAAGGATCCCATCCATGC+AGG - chr3.2:62054680-62054699 None:intergenic 50.0%
GCAACATGCCTGTTTGCTCA+TGG - chr3.2:62054655-62054674 None:intergenic 50.0%
GGGTGGTAATAAGTGCCCTT+GGG + chr3.2:62053988-62054007 MS.gene62244:intron 50.0%
GGTGGTAATAAGTGCCCTTG+GGG + chr3.2:62053989-62054008 MS.gene62244:intron 50.0%
GTTATTGCGATGCAGAGAGC+TGG + chr3.2:62053481-62053500 MS.gene62244:CDS 50.0%
TCTGTACGAGAAGAAGGCAG+AGG + chr3.2:62054259-62054278 MS.gene62244:CDS 50.0%
TGGCTAAGAAGAAAGCGGCT+TGG + chr3.2:62053596-62053615 MS.gene62244:CDS 50.0%
TGGGTGGTAATAAGTGCCCT+TGG + chr3.2:62053987-62054006 MS.gene62244:intron 50.0%
TTGAGCATTCCCAAGGGTCA+CGG - chr3.2:62054381-62054400 None:intergenic 50.0%
! AAGCAATTGGTGGATGTGCC+CGG + chr3.2:62053298-62053317 MS.gene62244:CDS 50.0%
! AATGGTGGGGGGATAACTGA+AGG + chr3.2:62054566-62054585 MS.gene62244:CDS 50.0%
! ACTTGCTGCTTCCATGACGA+TGG + chr3.2:62053174-62053193 MS.gene62244:CDS 50.0%
!! ATGGTGGTGATAATGGTGGG+GGG + chr3.2:62054555-62054574 MS.gene62244:CDS 50.0%
!! GAAGAAGGCTGTGGCTCTAA+GGG + chr3.2:62053642-62053661 MS.gene62244:CDS 50.0%
AAGGATCCCATCCATGCAGG+AGG - chr3.2:62054677-62054696 None:intergenic 55.0%
ACGCTCCTTGAGCATTCCCA+AGG - chr3.2:62054388-62054407 None:intergenic 55.0%
AGTTGAGGCGAAGAAGGCTG+TGG + chr3.2:62053633-62053652 MS.gene62244:CDS 55.0%
CAAGACGGTGCAGGATTGAC+TGG - chr3.2:62052945-62052964 None:intergenic 55.0%
CAGTCAATCCTGCACCGTCT+TGG + chr3.2:62052943-62052962 MS.gene62244:CDS 55.0%
CATGTTGCCTCCTGCATGGA+TGG + chr3.2:62054667-62054686 MS.gene62244:CDS 55.0%
CGCTCCTTGAGCATTCCCAA+GGG - chr3.2:62054387-62054406 None:intergenic 55.0%
GACCAATGGTGAAGACAGCG+GGG - chr3.2:62053036-62053055 None:intergenic 55.0%
GGACCAATGGTGAAGACAGC+GGG - chr3.2:62053037-62053056 None:intergenic 55.0%
GGCTGAAGTTGAGGCGAAGA+AGG + chr3.2:62053627-62053646 MS.gene62244:CDS 55.0%
GTGACCCTTGGGAATGCTCA+AGG + chr3.2:62054380-62054399 MS.gene62244:CDS 55.0%
TACCGGTGAGAGGTCCAAGA+CGG - chr3.2:62052960-62052979 None:intergenic 55.0%
! CGAAGAAGGCTGTGGCTCTA+AGG + chr3.2:62053641-62053660 MS.gene62244:CDS 55.0%
! GAAGAAAGCGGCTTGGACCA+AGG + chr3.2:62053603-62053622 MS.gene62244:CDS 55.0%
! TGCTGCTTCCATGACGATGG+AGG + chr3.2:62053177-62053196 MS.gene62244:CDS 55.0%
! TGGTCCTCGTGTTGACGATC+AGG + chr3.2:62053051-62053070 MS.gene62244:CDS 55.0%
AGCTGGAGAGGGTGAGAAGG+AGG + chr3.2:62053498-62053517 MS.gene62244:CDS 60.0%
CACCGTCTTGGACCTCTCAC+CGG + chr3.2:62052955-62052974 MS.gene62244:CDS 60.0%
CAGAGGGAACCGTGACCCTT+GGG + chr3.2:62054369-62054388 MS.gene62244:CDS 60.0%
CAGGCATGTTGCCTCCTGCA+TGG + chr3.2:62054663-62054682 MS.gene62244:CDS 60.0%
CTCAAGTCCGCGTATCGCCT+CGG - chr3.2:62054513-62054532 None:intergenic 60.0%
CTCCCCGCTGTCTTCACCAT+TGG + chr3.2:62053031-62053050 MS.gene62244:CDS 60.0%
GACCAAGGCGGCTGAAGTTG+AGG + chr3.2:62053618-62053637 MS.gene62244:CDS 60.0%
GAGAGCTGGAGAGGGTGAGA+AGG + chr3.2:62053495-62053514 MS.gene62244:CDS 60.0%
GCGATGCAGAGAGCTGGAGA+GGG + chr3.2:62053487-62053506 MS.gene62244:CDS 60.0%
GCTGGAGAGGGTGAGAAGGA+GGG + chr3.2:62053499-62053518 MS.gene62244:CDS 60.0%
TGAGAGGTCCAAGACGGTGC+AGG - chr3.2:62052954-62052973 None:intergenic 60.0%
TGCGATGCAGAGAGCTGGAG+AGG + chr3.2:62053486-62053505 MS.gene62244:CDS 60.0%
! GAAAGCGGCTTGGACCAAGG+CGG + chr3.2:62053606-62053625 MS.gene62244:CDS 60.0%
CGCCTCAACTTCAGCCGCCT+TGG - chr3.2:62053623-62053642 None:intergenic 65.0%
GCAGAGGGAACCGTGACCCT+TGG + chr3.2:62054368-62054387 MS.gene62244:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 62052845 62054715 62052845 ID=MS.gene62244
chr3.2 mRNA 62052845 62054715 62052845 ID=MS.gene62244.t1;Parent=MS.gene62244
chr3.2 exon 62052845 62053768 62052845 ID=MS.gene62244.t1.exon1;Parent=MS.gene62244.t1
chr3.2 CDS 62052845 62053768 62052845 ID=cds.MS.gene62244.t1;Parent=MS.gene62244.t1
chr3.2 exon 62054206 62054715 62054206 ID=MS.gene62244.t1.exon2;Parent=MS.gene62244.t1
chr3.2 CDS 62054206 62054715 62054206 ID=cds.MS.gene62244.t1;Parent=MS.gene62244.t1
Gene Sequence

>MS.gene62244

ATGAAGATTTACCGGGTCGCGAAAGCATCAGAATACCTTGTGATAACAGGTATTTTCATCAAAGACATCAAACTCAAAAAGAAAGCATGGATTTTTCCCAGTCAATCCTGCACCGTCTTGGACCTCTCACCGGTAAACTACACCTTCAAAGTTCAAGCTATGAGTGCCGAGAAACTTCCTTTTGTTCTCCCCGCTGTCTTCACCATTGGTCCTCGTGTTGACGATCAGGAAAGCCTTCTCAAATACGCCAAACTTATTTCACCACATGATCGACACTCCAATCACGTTAATGAACTTGTTCAAGGTATCATCGAAGGGGAGACTCGTGTACTTGCTGCTTCCATGACGATGGAGGAAGTTTTCAGAGGAACAAAACAGTTTAAACAAGAAGTATTCGACAAAGTTCAACTTGAGTTGAATCAATTCGGATTATTGATTTACAATGCGAATGTTAAGCAATTGGTGGATGTGCCCGGTCATGAGTATTTCTCATATTTGGGTCAAAAGACGCAGATGGAAGCCAAGAATCAAGCTAGGGTTGACGTGTCAGAAGCTAAGATGAAAGGTGAAATTGGTTCAAAATTGAGGGAAGGACAAACGCTGCAGAATGCAGCGAAAATTGATGCAGAGACGAAGGTTATTGCGATGCAGAGAGCTGGAGAGGGTGAGAAGGAGGGAATTAAAGTGAGGACTGAAGTGAAGGTTTTTGAGAATCAAAGAGAGGATGAAGTGGCTCAAGCTAATTCTGAGTTGGCTAAGAAGAAAGCGGCTTGGACCAAGGCGGCTGAAGTTGAGGCGAAGAAGGCTGTGGCTCTAAGGGAAGCTGAGTTGCAAGGTGAAGTTGAGAGGATGAATGCTTTGACTACAACTGAGAAGCTTAAAGCAGAGTTCCTTAGCAAAGCTAGTGTTCAATATGAAACCAAGGTTAGTTTAGTCCATGCAAATATAACTTCTTTCATTATATTCTTATGCAATTATTTTCTTTCATCAGCAACAAATTAATATATATATTATATAGGAGATTATTCAATATGGTTAAAGTTATTTTCATATATGTGATGAGAACAATGTCTGTTTGGGTGTTTAGGTTCATTTTATTGCTCTCTGTCACTTTCAAGTTTGACCAACATAGTCACGCTCAATGGGTGGTAATAAGTGCCCTTGGGGCATTGCTTAAGCATTCAAAAGTAGTAATCTAAAAAAAAAAAATTAGACTACTCCACTTGAATTGTTTTGTCTTGTCGCCATAAAAGTGTTCTATATAAGATGAATGATTTTCAACATTTACTTTTTAGGTGTTGGCTATTTGATAATTTTACTGGATTTTACTTTTTTGGTGATTAAAATTTTGGATGGTGTAGGTACAAGAAGCAAACTGGGAGCTGTACAAGAAACAGAAAGAGGCTGAAGCAATTCTGTACGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATCAGCAGATGCAACATTTTATGCTCGCAAGCAAGAAGCCGAAGCAGAGCTATATGCAAAGAAAAAAGAGGCAGAGGGAACCGTGACCCTTGGGAATGCTCAAGGAGCGTATGTAAGTACACTTCTCAATGCACTTGGAAACAACTACACAGCCGTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCCGAGGCGATACGCGGACTTGAGCCTAAGATTAGTATTTGGACTAATGGTGGTGATAATGGTGGGGGGATAACTGAAGGTGACATTGGTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTTTGTTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCTGCATGGATGGGATCCTTATCTGACAAAAGCTCTTAA
Protein sequence

>MS.gene62244.t1

MKIYRVAKASEYLVITGIFIKDIKLKKKAWIFPSQSCTVLDLSPVNYTFKVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDRHSNHVNELVQGIIEGETRVLAASMTMEEVFRGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAKNQARVDVSEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEGEKEGIKVRTEVKVFENQREDEVAQANSELAKKKAAWTKAAEVEAKKAVALREAELQGEVERMNALTTTEKLKAEFLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKASADATFYARKQEAEAELYAKKKEAEGTVTLGNAQGAYVSTLLNALGNNYTAVRDYLMINGGMFQEIAKINAEAIRGLEPKISIWTNGGDNGGGITEGDIGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGSLSDKSS