AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32671

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32671.t1 MTR_3g106480 99.582 478 2 0 1 478 1 478 0.0 970
MS.gene32671.t1 MTR_3g106485 94.142 478 24 2 1 478 1 474 0.0 887
MS.gene32671.t1 MTR_3g106420 91.841 478 39 0 1 478 3 480 0.0 877
MS.gene32671.t1 MTR_1g073400 90.795 478 40 1 1 478 1 474 0.0 847
MS.gene32671.t1 MTR_1g115145 88.935 479 46 3 1 478 1 473 0.0 844
MS.gene32671.t1 MTR_1g083190 89.749 478 41 1 1 478 3 472 0.0 842
MS.gene32671.t1 MTR_3g106430 92.034 477 34 2 1 477 1 473 0.0 840
MS.gene32671.t1 MTR_1g071130 85.983 478 38 2 1 478 1 449 0.0 837
MS.gene32671.t1 MTR_3g106470 80.922 477 35 2 1 477 32 452 0.0 737
MS.gene32671.t1 MTR_8g063830 80.215 465 51 5 1 464 1 425 0.0 668
MS.gene32671.t1 MTR_3g065540 65.217 115 13 1 40 154 14 101 8.63e-41 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32671.t1 AT5G25260 73.996 473 111 2 1 473 1 461 0.0 725
MS.gene32671.t1 AT5G25250 73.784 473 110 2 1 473 1 459 0.0 715
MS.gene32671.t1 AT5G64870 71.398 472 124 1 2 473 3 463 0.0 660

Find 121 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGAATATTTCTCATATT+TGG 0.052081 3.1:-84468132 MS.gene32671:CDS
AATTGAACCAATTCGGATTA+TGG 0.158331 3.1:-84468196 MS.gene32671:CDS
TTGAGCCTAAGATTAGTATT+TGG 0.251063 3.1:-84466502 MS.gene32671:CDS
CTGCTAAAGCCGTGGCTCTA+AGG 0.254538 3.1:-84467803 MS.gene32671:CDS
CATGAATATTTCTCATATTT+GGG 0.273088 3.1:-84468131 MS.gene32671:CDS
GGCTGGAGAAGGTGAGAAAC+AGG 0.294061 3.1:-84467955 MS.gene32671:CDS
GCTAAGATGAAAGGTGAAAT+TGG 0.319472 3.1:-84468056 MS.gene32671:CDS
TCTAGGTACAAGAGGCTAAC+TGG 0.321242 3.1:-84466829 MS.gene32671:intron
AACGAGTTCATTAACGTGAT+TGG 0.339850 3.1:+84468331 None:intergenic
CAACTTGAATTGAACCAATT+CGG 0.339963 3.1:-84468203 MS.gene32671:CDS
CATGTTGCCTCCGGCTTGGA+TGG 0.343865 3.1:-84466363 MS.gene32671:CDS
CAATGCCAATGTTAAGCAAT+TGG 0.346020 3.1:-84468168 MS.gene32671:CDS
AGGGATTACTTGATGATAAA+TGG 0.347159 3.1:-84466575 MS.gene32671:CDS
GTGAAATTGGTTCGAAATTG+AGG 0.356446 3.1:-84468043 MS.gene32671:CDS
ATCATGAGGGGAAATTAGTT+TGG 0.360315 3.1:+84468361 None:intergenic
TCAGAATACCTTGTGATAAC+TGG 0.379855 3.1:-84468581 MS.gene32671:CDS
TTAGTCCAAATACTAATCTT+AGG 0.384650 3.1:+84466497 None:intergenic
AAGATTAGTATTTGGACTAA+TGG 0.384982 3.1:-84466494 MS.gene32671:CDS
AAGCTTTACATCGTCAATTC+CGG 0.391365 3.1:+84468556 None:intergenic
TGTAAATCCATAATCCGAAT+TGG 0.394399 3.1:+84468189 None:intergenic
CTCCCTGCCGTCTTCACAAT+AGG 0.407375 3.1:-84468422 MS.gene32671:CDS
AAATTAGTTTGGCATATTTG+AGG 0.439485 3.1:+84468372 None:intergenic
CTAATGGTGGTGATAATAAC+GGG 0.457145 3.1:-84466478 MS.gene32671:CDS
CACGTTAATGAACTCGTTCA+AGG 0.459703 3.1:-84468326 MS.gene32671:CDS
AGCAATTCTGTATGAGAAGA+AGG 0.462456 3.1:-84466777 MS.gene32671:CDS
GGGAATAACTGAAGGTGCCA+TGG 0.466350 3.1:-84466456 MS.gene32671:CDS
TGAAATTGGTTCGAAATTGA+GGG 0.484739 3.1:-84468042 MS.gene32671:CDS
AAACCAATATCAACATGTAC+CGG 0.488341 3.1:-84468616 None:intergenic
CTCAAGTCCGCGTACCGCCT+CGG 0.491620 3.1:+84466520 None:intergenic
AAGACTGTCCATGAGCAAAC+AGG 0.492575 3.1:-84466386 MS.gene32671:CDS
TGCTTCAATGACAATGGAAG+AGG 0.493478 3.1:-84468273 MS.gene32671:CDS
TGCTAAAGCCGTGGCTCTAA+GGG 0.495165 3.1:-84467802 MS.gene32671:CDS
GGAACAACTACACAGCTGTA+AGG 0.502820 3.1:-84466595 MS.gene32671:CDS
CTAGGTACAAGAGGCTAACT+GGG 0.505098 3.1:-84466828 MS.gene32671:intron
TGGAAGCAGCGAATCAAGCT+AGG 0.506083 3.1:-84468094 MS.gene32671:CDS
GGACCTATTGTGAAGACGGC+AGG 0.507327 3.1:+84468419 None:intergenic
ATGTTGCCTCCGGCTTGGAT+GGG 0.512421 3.1:-84466362 MS.gene32671:CDS
TTGGACAATGGCGGCTCAAG+TGG 0.512749 3.1:-84467838 MS.gene32671:CDS
GTTATTGCGATGCAGAGGGC+TGG 0.513050 3.1:-84467972 MS.gene32671:CDS
CAGGCATGTTGCCTCCGGCT+TGG 0.517134 3.1:-84466367 MS.gene32671:CDS
GCAACATGCCTGTTTGCTCA+TGG 0.521020 3.1:+84466378 None:intergenic
AGCTTTAAGCTTCTCAGTAG+TGG 0.525292 3.1:+84467737 None:intergenic
AAGCAATTGGTGGATGTGCC+TGG 0.526695 3.1:-84468155 MS.gene32671:CDS
GGCTCAAGTGGCTGAGCTTG+AGG 0.528141 3.1:-84467826 MS.gene32671:CDS
GCAAACAGGCATGTTGCCTC+CGG 0.529165 3.1:-84466372 MS.gene32671:CDS
ATTAGTATTTGGACTAATGG+TGG 0.532835 3.1:-84466491 MS.gene32671:CDS
AGCAACTTCTTTCATACCCA+TGG 0.534773 3.1:+84466439 None:intergenic
ATCCACCAATTGCTTAACAT+TGG 0.540817 3.1:+84468163 None:intergenic
AGTACACTTCTCAATGCACT+TGG 0.541119 3.1:-84466617 MS.gene32671:CDS
CACCGTCTTCGACCTCTCAC+CGG 0.542934 3.1:-84468498 MS.gene32671:CDS
ATTCAACACTAGCTTTGCTA+AGG 0.544556 3.1:+84467709 None:intergenic
TGAGAGGTCGAAGACGGTGC+AGG 0.545239 3.1:+84468502 None:intergenic
GGAATAACTGAAGGTGCCAT+GGG 0.546476 3.1:-84466455 MS.gene32671:CDS
GCAGAGGGGATTGTGACACT+TGG 0.546780 3.1:-84466662 MS.gene32671:CDS
ACTTCGAAGGTGTAATTCAC+CGG 0.552954 3.1:+84468479 None:intergenic
AAGACGGTGCAGGATTGACC+CGG 0.554154 3.1:+84468512 None:intergenic
AAGAGAGGCTGAAGTGGCTG+AGG 0.558698 3.1:-84467892 MS.gene32671:CDS
GAAGAAAGCTGCTTGGACAA+TGG 0.561707 3.1:-84467850 MS.gene32671:CDS
AGCTTTCTTCTTAGCCAACT+CGG 0.562096 3.1:+84467863 None:intergenic
TAGTGTTGAATATGACACTA+AGG 0.562966 3.1:-84467697 MS.gene32671:intron
TAAAGCTTGAAAAGAAAGCA+TGG 0.566286 3.1:-84468541 MS.gene32671:CDS
AAGACGGCAGGGAGAACGAA+AGG 0.568717 3.1:+84468431 None:intergenic
TGGCTAAGAAGAAAGCTGCT+TGG 0.570632 3.1:-84467857 MS.gene32671:CDS
GGCTGAGGCTAATTCCGAGT+TGG 0.571047 3.1:-84467877 MS.gene32671:CDS
GGTAAAGATCCCATCCAAGC+CGG 0.577737 3.1:+84466353 None:intergenic
ATGGGTATGAAAGAAGTTGC+TGG 0.579940 3.1:-84466437 MS.gene32671:CDS
TACCTTGTGATAACTGGCGC+CGG 0.580254 3.1:-84468575 MS.gene32671:CDS
TGCCAATGTTAAGCAATTGG+TGG 0.581862 3.1:-84468165 MS.gene32671:CDS
GTGACACTTGGGAATGCTCA+AGG 0.583871 3.1:-84466650 MS.gene32671:CDS
ACTAATGGTGGTGATAATAA+CGG 0.586869 3.1:-84466479 MS.gene32671:CDS
CGAAGGTTATTGCGATGCAG+AGG 0.587186 3.1:-84467977 MS.gene32671:CDS
CTGGATGCTCTAGGTACAAG+AGG 0.587653 3.1:-84466837 MS.gene32671:intron
GAATCAAAGAGAGGCTGAAG+TGG 0.589954 3.1:-84467898 MS.gene32671:CDS
CTCAGCTTCCCTTAGAGCCA+CGG 0.590772 3.1:+84467794 None:intergenic
AGGTGTAATTCACCGGTGAG+AGG 0.592989 3.1:+84468486 None:intergenic
GAACAACTACACAGCTGTAA+GGG 0.593770 3.1:-84466594 MS.gene32671:CDS
ACTCATGGCTTGAACTTCGA+AGG 0.596495 3.1:+84468466 None:intergenic
CCTCGTTGCTTCAATGACAA+TGG 0.596534 3.1:-84468279 MS.gene32671:CDS
TTAGTTTGGCATATTTGAGG+AGG 0.598221 3.1:+84468375 None:intergenic
ACGAGGACCTATTGTGAAGA+CGG 0.598551 3.1:+84468415 None:intergenic
CAGAGGGGATTGTGACACTT+GGG 0.598815 3.1:-84466661 MS.gene32671:CDS
TCTGTATGAGAAGAAGGCAG+AGG 0.600808 3.1:-84466771 MS.gene32671:CDS
GCGATGCAGAGGGCTGGAGA+AGG 0.602267 3.1:-84467966 MS.gene32671:CDS
GACCTATTGTGAAGACGGCA+GGG 0.603396 3.1:+84468420 None:intergenic
GATTGGAGAGTTTATCATGA+GGG 0.606472 3.1:+84468348 None:intergenic
CTCGTTCAAGGAATCATCGA+AGG 0.607063 3.1:-84468314 MS.gene32671:CDS
AGTTGCAAGGTGAGGTTGAG+AGG 0.610701 3.1:-84467773 MS.gene32671:CDS
CTAAGGGAAGCTGAGTTGCA+AGG 0.613783 3.1:-84467786 MS.gene32671:CDS
GTGGCAGAAGCTAAGATGAA+AGG 0.614706 3.1:-84468065 MS.gene32671:CDS
ATTGGAGAGTTTATCATGAG+GGG 0.615848 3.1:+84468349 None:intergenic
TTTGGGACAAAAGACGCAGA+TGG 0.620243 3.1:-84468114 MS.gene32671:CDS
TTGTTTGCGAGCATAAAATG+TGG 0.626952 3.1:+84466721 None:intergenic
CACCGGTGAGAGGTCGAAGA+CGG 0.628279 3.1:+84468496 None:intergenic
ATTGGTTCGAAATTGAGGGA+AGG 0.631232 3.1:-84468038 MS.gene32671:CDS
AGACGGCAGGGAGAACGAAA+GGG 0.631236 3.1:+84468432 None:intergenic
GCTTGAGGCTGCTAAAGCCG+TGG 0.632981 3.1:-84467811 MS.gene32671:CDS
GAAGGTTATTGCGATGCAGA+GGG 0.633633 3.1:-84467976 MS.gene32671:CDS
TATGAGAAATATTCATGACC+AGG 0.636387 3.1:+84468137 None:intergenic
GGAAGCAGCGAATCAAGCTA+GGG 0.637623 3.1:-84468093 MS.gene32671:CDS
GATTGCTAAGATTAATGCCG+AGG 0.643593 3.1:-84466537 MS.gene32671:CDS
GGAAGCTGAGTTGCAAGGTG+AGG 0.648282 3.1:-84467781 MS.gene32671:CDS
AACCAATATCAACATGTACC+GGG 0.649459 3.1:-84468615 None:intergenic
GAAAATTGATGCAGAGACGA+AGG 0.652587 3.1:-84467994 MS.gene32671:CDS
TTCCGGCGCCAGTTATCACA+AGG 0.656470 3.1:+84468573 None:intergenic
TGGACAGTCTTAAACAGAGG+TGG 0.656626 3.1:+84466398 None:intergenic
GATTACTTGATGATAAATGG+TGG 0.658453 3.1:-84466572 MS.gene32671:CDS
TCATGGACAGTCTTAAACAG+AGG 0.660499 3.1:+84466395 None:intergenic
TGCTAAGATTAATGCCGAGG+CGG 0.661554 3.1:-84466534 MS.gene32671:CDS
AGACGGTGCAGGATTGACCC+GGG 0.662490 3.1:+84468513 None:intergenic
GTACACTTCTCAATGCACTT+GGG 0.664100 3.1:-84466616 MS.gene32671:CDS
GAAAGCTGCTTGGACAATGG+CGG 0.679670 3.1:-84467847 MS.gene32671:CDS
TGATTGGAGAGTTTATCATG+AGG 0.680440 3.1:+84468347 None:intergenic
ATTAATGCCGAGGCGGTACG+CGG 0.680968 3.1:-84466527 MS.gene32671:CDS
GAATCAAGCTAGGGTTGACG+TGG 0.682564 3.1:-84468084 MS.gene32671:CDS
GCTGGAGAAGGTGAGAAACA+GGG 0.684270 3.1:-84467954 MS.gene32671:CDS
AAAGATCCCATCCAAGCCGG+AGG 0.687910 3.1:+84466356 None:intergenic
AGAAACAGGGAATTAAAGTG+AGG 0.688934 3.1:-84467941 MS.gene32671:CDS
CTTTCGTAATCGTCAACACG+AGG 0.692794 3.1:+84468398 None:intergenic
CCATTGTCATTGAAGCAACG+AGG 0.710320 3.1:+84468279 None:intergenic
AATGGTGGTGATAATAACGG+GGG 0.719005 3.1:-84466476 MS.gene32671:CDS
TAATGGTGGTGATAATAACG+GGG 0.724495 3.1:-84466477 MS.gene32671:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTATAAAATTAAATCATT+CGG - chr3.1:84467759-84467778 MS.gene32671:CDS 10.0%
!!! ATTAAAAATCTTCTTTTTTT+TGG + chr3.1:84468054-84468073 None:intergenic 10.0%
!!! TATGTTTTACTTTTAATCAA+CGG + chr3.1:84467986-84468005 None:intergenic 15.0%
!! AAAAGTAAAACATAACAAGT+AGG - chr3.1:84467992-84468011 MS.gene32671:CDS 20.0%
!! AATGAGACTATTATATATTG+AGG - chr3.1:84467385-84467404 MS.gene32671:intron 20.0%
!! ATTATACAAGAAACAAAAAG+AGG - chr3.1:84468135-84468154 MS.gene32671:CDS 20.0%
!! CATGAATATTTCTCATATTT+GGG - chr3.1:84466808-84466827 MS.gene32671:CDS 20.0%
!! CCACAATATAGTTTAATAAA+AGG + chr3.1:84467578-84467597 None:intergenic 20.0%
!! TCATGAATATTTCTCATATT+TGG - chr3.1:84466807-84466826 MS.gene32671:CDS 20.0%
!! TTAAATTCATACATATACCT+AGG + chr3.1:84467439-84467458 None:intergenic 20.0%
!!! AAAAATCTTCTTTTTTTTGG+TGG + chr3.1:84468051-84468070 None:intergenic 20.0%
!!! CCTTTTATTAAACTATATTG+TGG - chr3.1:84467575-84467594 MS.gene32671:intron 20.0%
! AAGAAAAAGATTGAAGAATG+TGG - chr3.1:84467919-84467938 MS.gene32671:CDS 25.0%
! AAGAAGAAGTTAAATTTGCA+AGG + chr3.1:84467273-84467292 None:intergenic 25.0%
! AAGATTAGTATTTGGACTAA+TGG - chr3.1:84468445-84468464 MS.gene32671:CDS 25.0%
! ACACAAATAATAGCAAGAAA+AGG + chr3.1:84467849-84467868 None:intergenic 25.0%
! ATATTGAAACCAATCACAAA+GGG + chr3.1:84467474-84467493 None:intergenic 25.0%
! ATGATATATGTTCATTTGCA+AGG + chr3.1:84467722-84467741 None:intergenic 25.0%
! GTATATTAACTATGGATTTG+CGG + chr3.1:84467700-84467719 None:intergenic 25.0%
! TATATTGAAACCAATCACAA+AGG + chr3.1:84467475-84467494 None:intergenic 25.0%
! TTAACAAAAATTGACAAGGT+TGG + chr3.1:84467956-84467975 None:intergenic 25.0%
! TTAGTCCAAATACTAATCTT+AGG + chr3.1:84468445-84468464 None:intergenic 25.0%
! TTTGCAAGGTATATTAACTA+TGG + chr3.1:84467708-84467727 None:intergenic 25.0%
!! AAATTAGTTTGGCATATTTG+AGG + chr3.1:84466570-84466589 None:intergenic 25.0%
!! TAATGTTTAATCTGTCGATT+GGG + chr3.1:84467645-84467664 None:intergenic 25.0%
!!! AATGTTTACTTTTTAAGTGC+TGG - chr3.1:84468071-84468090 MS.gene32671:CDS 25.0%
!!! TCACTGTAATTACAGTTTTT+TGG - chr3.1:84467353-84467372 MS.gene32671:intron 25.0%
AAAGGATCATGATTTAGACT+TGG + chr3.1:84467831-84467850 None:intergenic 30.0%
AAGTAGCAAAATGCTAATTG+AGG - chr3.1:84467674-84467693 MS.gene32671:intron 30.0%
AATTGAACCAATTCGGATTA+TGG - chr3.1:84466743-84466762 MS.gene32671:CDS 30.0%
ATTAGTATTTGGACTAATGG+TGG - chr3.1:84468448-84468467 MS.gene32671:CDS 30.0%
CAACTTGAATTGAACCAATT+CGG - chr3.1:84466736-84466755 MS.gene32671:CDS 30.0%
CCTGTTAACAAAAATTGACA+AGG + chr3.1:84467960-84467979 None:intergenic 30.0%
GTAATGTTTAATCTGTCGAT+TGG + chr3.1:84467646-84467665 None:intergenic 30.0%
GTTATACGCAAAGAAAAAAG+AGG - chr3.1:84468255-84468274 MS.gene32671:CDS 30.0%
TAAAGCTTGAAAAGAAAGCA+TGG - chr3.1:84466398-84466417 MS.gene32671:CDS 30.0%
TATGAGAAATATTCATGACC+AGG + chr3.1:84466805-84466824 None:intergenic 30.0%
TATTGAAACCAATCACAAAG+GGG + chr3.1:84467473-84467492 None:intergenic 30.0%
TATTTACGAAGATCATAGGA+CGG - chr3.1:84467521-84467540 MS.gene32671:intron 30.0%
TGCATATTTACGAAGATCAT+AGG - chr3.1:84467517-84467536 MS.gene32671:intron 30.0%
TGTAAATCCATAATCCGAAT+TGG + chr3.1:84466753-84466772 None:intergenic 30.0%
TTGAGCCTAAGATTAGTATT+TGG - chr3.1:84468437-84468456 MS.gene32671:CDS 30.0%
! AACTTTAAGTTTAAGCTCCT+AGG - chr3.1:84467419-84467438 MS.gene32671:intron 30.0%
! AATGTTTAATCTGTCGATTG+GGG + chr3.1:84467644-84467663 None:intergenic 30.0%
! ACTAATGGTGGTGATAATAA+CGG - chr3.1:84468460-84468479 MS.gene32671:CDS 30.0%
! AGGGATTACTTGATGATAAA+TGG - chr3.1:84468364-84468383 MS.gene32671:CDS 30.0%
! CCTTGTCAATTTTTGTTAAC+AGG - chr3.1:84467957-84467976 MS.gene32671:CDS 30.0%
! GATTACTTGATGATAAATGG+TGG - chr3.1:84468367-84468386 MS.gene32671:CDS 30.0%
! TAGTGTTGAATATGACACTA+AGG - chr3.1:84467242-84467261 MS.gene32671:intron 30.0%
!! AGTTTTTGAGAATCAAAGAG+AGG - chr3.1:84467032-84467051 MS.gene32671:intron 30.0%
!! TGAAATTGGTTCGAAATTGA+GGG - chr3.1:84466897-84466916 MS.gene32671:intron 30.0%
!!! AATCTTCTTTTTTTTGGTGG+TGG + chr3.1:84468048-84468067 None:intergenic 30.0%
AACGAGTTCATTAACGTGAT+TGG + chr3.1:84466611-84466630 None:intergenic 35.0%
AAGCTTTACATCGTCAATTC+CGG + chr3.1:84466386-84466405 None:intergenic 35.0%
ACTATATTGTGGTTGCAGAT+AGG - chr3.1:84467586-84467605 MS.gene32671:intron 35.0%
AGAAACAGGGAATTAAAGTG+AGG - chr3.1:84466998-84467017 MS.gene32671:intron 35.0%
AGCAATTCTGTATGAGAAGA+AGG - chr3.1:84468162-84468181 MS.gene32671:CDS 35.0%
ATCATGAGGGGAAATTAGTT+TGG + chr3.1:84466581-84466600 None:intergenic 35.0%
ATCCACCAATTGCTTAACAT+TGG + chr3.1:84466779-84466798 None:intergenic 35.0%
ATTGAAACCAATCACAAAGG+GGG + chr3.1:84467472-84467491 None:intergenic 35.0%
ATTGGAGAGTTTATCATGAG+GGG + chr3.1:84466593-84466612 None:intergenic 35.0%
ATTTACGAAGATCATAGGAC+GGG - chr3.1:84467522-84467541 MS.gene32671:intron 35.0%
CAATGCCAATGTTAAGCAAT+TGG - chr3.1:84466771-84466790 MS.gene32671:CDS 35.0%
GACGGGAGTTAAAAATTCAA+TGG - chr3.1:84467539-84467558 MS.gene32671:intron 35.0%
GATTGGAGAGTTTATCATGA+GGG + chr3.1:84466594-84466613 None:intergenic 35.0%
GCTAAGATGAAAGGTGAAAT+TGG - chr3.1:84466883-84466902 MS.gene32671:intron 35.0%
TCAGAATACCTTGTGATAAC+TGG - chr3.1:84466358-84466377 MS.gene32671:CDS 35.0%
TGATTGGAGAGTTTATCATG+AGG + chr3.1:84466595-84466614 None:intergenic 35.0%
TTGTTTGCGAGCATAAAATG+TGG + chr3.1:84468221-84468240 None:intergenic 35.0%
! ATGTTTAATCTGTCGATTGG+GGG + chr3.1:84467643-84467662 None:intergenic 35.0%
! CTAATGGTGGTGATAATAAC+GGG - chr3.1:84468461-84468480 MS.gene32671:CDS 35.0%
! TAATGGTGGTGATAATAACG+GGG - chr3.1:84468462-84468481 MS.gene32671:CDS 35.0%
! TATTTTACCCCCTTTGTGAT+TGG - chr3.1:84467462-84467481 MS.gene32671:intron 35.0%
! TTAGTTTGGCATATTTGAGG+AGG + chr3.1:84466567-84466586 None:intergenic 35.0%
!! AAAGAAAGCATGGATTTTCC+CGG - chr3.1:84466408-84466427 MS.gene32671:CDS 35.0%
!! ATTCAACACTAGCTTTGCTA+AGG + chr3.1:84467233-84467252 None:intergenic 35.0%
!! GTGAAATTGGTTCGAAATTG+AGG - chr3.1:84466896-84466915 MS.gene32671:intron 35.0%
!! TTAAGTGCTGGTTGAAATTC+TGG - chr3.1:84468083-84468102 MS.gene32671:CDS 35.0%
AGCAACTTCTTTCATACCCA+TGG + chr3.1:84468503-84468522 None:intergenic 40.0%
AGCTTTCTTCTTAGCCAACT+CGG + chr3.1:84467079-84467098 None:intergenic 40.0%
AGTACACTTCTCAATGCACT+TGG - chr3.1:84468322-84468341 MS.gene32671:CDS 40.0%
ATGGGTATGAAAGAAGTTGC+TGG - chr3.1:84468502-84468521 MS.gene32671:CDS 40.0%
CAAAGAAAAAAGAGGCAGAG+GGG - chr3.1:84468263-84468282 MS.gene32671:CDS 40.0%
CACGTTAATGAACTCGTTCA+AGG - chr3.1:84466613-84466632 MS.gene32671:CDS 40.0%
CTTCGTAAATATGCAGTGCA+CGG + chr3.1:84467512-84467531 None:intergenic 40.0%
GAAAATTGATGCAGAGACGA+AGG - chr3.1:84466945-84466964 MS.gene32671:intron 40.0%
GAACAACTACACAGCTGTAA+GGG - chr3.1:84468345-84468364 MS.gene32671:CDS 40.0%
GATTGCTAAGATTAATGCCG+AGG - chr3.1:84468402-84468421 MS.gene32671:CDS 40.0%
GCAAAGAAAAAAGAGGCAGA+GGG - chr3.1:84468262-84468281 MS.gene32671:CDS 40.0%
GTACACTTCTCAATGCACTT+GGG - chr3.1:84468323-84468342 MS.gene32671:CDS 40.0%
GTTGAAATTCTGGATGCTCT+AGG - chr3.1:84468093-84468112 MS.gene32671:CDS 40.0%
TCATGGACAGTCTTAAACAG+AGG + chr3.1:84468547-84468566 None:intergenic 40.0%
TGCCAATGTTAAGCAATTGG+TGG - chr3.1:84466774-84466793 MS.gene32671:CDS 40.0%
TGCTTCAATGACAATGGAAG+AGG - chr3.1:84466666-84466685 MS.gene32671:CDS 40.0%
TGTTGAGTTAGCAAGTGCTA+TGG - chr3.1:84467884-84467903 MS.gene32671:CDS 40.0%
! AATAACGGGGGAATAACTGA+AGG - chr3.1:84468475-84468494 MS.gene32671:CDS 40.0%
! AATGGTGGTGATAATAACGG+GGG - chr3.1:84468463-84468482 MS.gene32671:CDS 40.0%
! ACAATGGAAGAGGTTTTCAG+AGG - chr3.1:84466676-84466695 MS.gene32671:CDS 40.0%
! ACTTCGAAGGTGTAATTCAC+CGG + chr3.1:84466463-84466482 None:intergenic 40.0%
! ATTCTGATGCTTTTGCTACC+CGG + chr3.1:84466345-84466364 None:intergenic 40.0%
!! AAGAAAGCATGGATTTTCCC+GGG - chr3.1:84466409-84466428 MS.gene32671:CDS 40.0%
!! AGCTTTAAGCTTCTCAGTAG+TGG + chr3.1:84467205-84467224 None:intergenic 40.0%
!! ATTGGTTCGAAATTGAGGGA+AGG - chr3.1:84466901-84466920 MS.gene32671:intron 40.0%
AAGACTGTCCATGAGCAAAC+AGG - chr3.1:84468553-84468572 MS.gene32671:CDS 45.0%
ACGAGGACCTATTGTGAAGA+CGG + chr3.1:84466527-84466546 None:intergenic 45.0%
ACTCATGGCTTGAACTTCGA+AGG + chr3.1:84466476-84466495 None:intergenic 45.0%
CCATTGTCATTGAAGCAACG+AGG + chr3.1:84466663-84466682 None:intergenic 45.0%
CCTCGTTGCTTCAATGACAA+TGG - chr3.1:84466660-84466679 MS.gene32671:CDS 45.0%
CGCAAAGAAAAAAGAGGCAG+AGG - chr3.1:84468261-84468280 MS.gene32671:CDS 45.0%
CTAGGTACAAGAGGCTAACT+GGG - chr3.1:84468111-84468130 MS.gene32671:CDS 45.0%
CTCGTTCAAGGAATCATCGA+AGG - chr3.1:84466625-84466644 MS.gene32671:CDS 45.0%
CTTTCGTAATCGTCAACACG+AGG + chr3.1:84466544-84466563 None:intergenic 45.0%
GAAGAAAGCTGCTTGGACAA+TGG - chr3.1:84467089-84467108 MS.gene32671:intron 45.0%
GAAGGTTATTGCGATGCAGA+GGG - chr3.1:84466963-84466982 MS.gene32671:intron 45.0%
GAATCAAAGAGAGGCTGAAG+TGG - chr3.1:84467041-84467060 MS.gene32671:intron 45.0%
GGAACAACTACACAGCTGTA+AGG - chr3.1:84468344-84468363 MS.gene32671:CDS 45.0%
GTGGCAGAAGCTAAGATGAA+AGG - chr3.1:84466874-84466893 MS.gene32671:intron 45.0%
TCTAGGTACAAGAGGCTAAC+TGG - chr3.1:84468110-84468129 MS.gene32671:CDS 45.0%
TCTGTATGAGAAGAAGGCAG+AGG - chr3.1:84468168-84468187 MS.gene32671:CDS 45.0%
TGCTAAGATTAATGCCGAGG+CGG - chr3.1:84468405-84468424 MS.gene32671:CDS 45.0%
TGGACAGTCTTAAACAGAGG+TGG + chr3.1:84468544-84468563 None:intergenic 45.0%
TGGCTAAGAAGAAAGCTGCT+TGG - chr3.1:84467082-84467101 MS.gene32671:intron 45.0%
! AGGGAGTTTTTCTGCACTCA+TGG + chr3.1:84466491-84466510 None:intergenic 45.0%
! CATCAATTTTCGCTGCGTTC+TGG + chr3.1:84466936-84466955 None:intergenic 45.0%
! TTTGGGACAAAAGACGCAGA+TGG - chr3.1:84466825-84466844 MS.gene32671:CDS 45.0%
!! GGAATAACTGAAGGTGCCAT+GGG - chr3.1:84468484-84468503 MS.gene32671:CDS 45.0%
AATATGCAGTGCACGGACGT+AGG + chr3.1:84467505-84467524 None:intergenic 50.0%
AGGTGTAATTCACCGGTGAG+AGG + chr3.1:84466456-84466475 None:intergenic 50.0%
AGTTGCAAGGTGAGGTTGAG+AGG - chr3.1:84467166-84467185 MS.gene32671:intron 50.0%
CAGAGGGGATTGTGACACTT+GGG - chr3.1:84468278-84468297 MS.gene32671:CDS 50.0%
CGAAGGTTATTGCGATGCAG+AGG - chr3.1:84466962-84466981 MS.gene32671:intron 50.0%
CTAAGGGAAGCTGAGTTGCA+AGG - chr3.1:84467153-84467172 MS.gene32671:intron 50.0%
GAAAGCTGCTTGGACAATGG+CGG - chr3.1:84467092-84467111 MS.gene32671:intron 50.0%
GAATCAAGCTAGGGTTGACG+TGG - chr3.1:84466855-84466874 MS.gene32671:intron 50.0%
GACCTATTGTGAAGACGGCA+GGG + chr3.1:84466522-84466541 None:intergenic 50.0%
GCAACATGCCTGTTTGCTCA+TGG + chr3.1:84468564-84468583 None:intergenic 50.0%
GCTGGAGAAGGTGAGAAACA+GGG - chr3.1:84466985-84467004 MS.gene32671:intron 50.0%
GGAAGCAGCGAATCAAGCTA+GGG - chr3.1:84466846-84466865 MS.gene32671:intron 50.0%
GGTAAAGATCCCATCCAAGC+CGG + chr3.1:84468589-84468608 None:intergenic 50.0%
GTGACACTTGGGAATGCTCA+AGG - chr3.1:84468289-84468308 MS.gene32671:CDS 50.0%
TACCTTGTGATAACTGGCGC+CGG - chr3.1:84466364-84466383 MS.gene32671:CDS 50.0%
TGGAAGCAGCGAATCAAGCT+AGG - chr3.1:84466845-84466864 MS.gene32671:intron 50.0%
! AAGCAATTGGTGGATGTGCC+TGG - chr3.1:84466784-84466803 MS.gene32671:CDS 50.0%
! CTGGATGCTCTAGGTACAAG+AGG - chr3.1:84468102-84468121 MS.gene32671:CDS 50.0%
! GGCAGAAGCGCAAAAAGCTT+TGG - chr3.1:84468189-84468208 MS.gene32671:CDS 50.0%
!! GGGAATAACTGAAGGTGCCA+TGG - chr3.1:84468483-84468502 MS.gene32671:CDS 50.0%
!! TGCTAAAGCCGTGGCTCTAA+GGG - chr3.1:84467137-84467156 MS.gene32671:intron 50.0%
AAAGATCCCATCCAAGCCGG+AGG + chr3.1:84468586-84468605 None:intergenic 55.0%
AAGACGGCAGGGAGAACGAA+AGG + chr3.1:84466511-84466530 None:intergenic 55.0%
AAGACGGTGCAGGATTGACC+CGG + chr3.1:84466430-84466449 None:intergenic 55.0%
AAGAGAGGCTGAAGTGGCTG+AGG - chr3.1:84467047-84467066 MS.gene32671:intron 55.0%
AGACGGCAGGGAGAACGAAA+GGG + chr3.1:84466510-84466529 None:intergenic 55.0%
ATTAATGCCGAGGCGGTACG+CGG - chr3.1:84468412-84468431 MS.gene32671:CDS 55.0%
CTCAGCTTCCCTTAGAGCCA+CGG + chr3.1:84467148-84467167 None:intergenic 55.0%
CTCCCTGCCGTCTTCACAAT+AGG - chr3.1:84466517-84466536 MS.gene32671:CDS 55.0%
GCAAACAGGCATGTTGCCTC+CGG - chr3.1:84468567-84468586 MS.gene32671:CDS 55.0%
GCAGAGGGGATTGTGACACT+TGG - chr3.1:84468277-84468296 MS.gene32671:CDS 55.0%
GGAAGCTGAGTTGCAAGGTG+AGG - chr3.1:84467158-84467177 MS.gene32671:intron 55.0%
GGACCTATTGTGAAGACGGC+AGG + chr3.1:84466523-84466542 None:intergenic 55.0%
GGCTGAGGCTAATTCCGAGT+TGG - chr3.1:84467062-84467081 MS.gene32671:intron 55.0%
GGCTGGAGAAGGTGAGAAAC+AGG - chr3.1:84466984-84467003 MS.gene32671:intron 55.0%
GTTATTGCGATGCAGAGGGC+TGG - chr3.1:84466967-84466986 MS.gene32671:intron 55.0%
TTCCGGCGCCAGTTATCACA+AGG + chr3.1:84466369-84466388 None:intergenic 55.0%
TTGGACAATGGCGGCTCAAG+TGG - chr3.1:84467101-84467120 MS.gene32671:intron 55.0%
! CTGCTAAAGCCGTGGCTCTA+AGG - chr3.1:84467136-84467155 MS.gene32671:intron 55.0%
!! ATGTTGCCTCCGGCTTGGAT+GGG - chr3.1:84468577-84468596 MS.gene32671:CDS 55.0%
AGACGGTGCAGGATTGACCC+GGG + chr3.1:84466429-84466448 None:intergenic 60.0%
CACCGGTGAGAGGTCGAAGA+CGG + chr3.1:84466446-84466465 None:intergenic 60.0%
CACCGTCTTCGACCTCTCAC+CGG - chr3.1:84466441-84466460 MS.gene32671:CDS 60.0%
GCTTGAGGCTGCTAAAGCCG+TGG - chr3.1:84467128-84467147 MS.gene32671:intron 60.0%
GGCTCAAGTGGCTGAGCTTG+AGG - chr3.1:84467113-84467132 MS.gene32671:intron 60.0%
TGAGAGGTCGAAGACGGTGC+AGG + chr3.1:84466440-84466459 None:intergenic 60.0%
!! CATGTTGCCTCCGGCTTGGA+TGG - chr3.1:84468576-84468595 MS.gene32671:CDS 60.0%
CAGGCATGTTGCCTCCGGCT+TGG - chr3.1:84468572-84468591 MS.gene32671:CDS 65.0%
CTCAAGTCCGCGTACCGCCT+CGG + chr3.1:84468422-84468441 None:intergenic 65.0%
GCGATGCAGAGGGCTGGAGA+AGG - chr3.1:84466973-84466992 MS.gene32671:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84466337 84468624 84466337 ID=MS.gene32671
chr3.1 mRNA 84466337 84468624 84466337 ID=MS.gene32671.t1;Parent=MS.gene32671
chr3.1 exon 84467698 84468624 84467698 ID=MS.gene32671.t1.exon1;Parent=MS.gene32671.t1
chr3.1 CDS 84467698 84468624 84467698 ID=cds.MS.gene32671.t1;Parent=MS.gene32671.t1
chr3.1 exon 84466337 84466846 84466337 ID=MS.gene32671.t1.exon2;Parent=MS.gene32671.t1
chr3.1 CDS 84466337 84466846 84466337 ID=cds.MS.gene32671.t1;Parent=MS.gene32671.t1
Gene Sequence

>MS.gene32671

ATGTACCGGGTAGCAAAAGCATCAGAATACCTTGTGATAACTGGCGCCGGAATTGACGATGTAAAGCTTGAAAAGAAAGCATGGATTTTCCCGGGTCAATCCTGCACCGTCTTCGACCTCTCACCGGTGAATTACACCTTCGAAGTTCAAGCCATGAGTGCAGAAAAACTCCCTTTCGTTCTCCCTGCCGTCTTCACAATAGGTCCTCGTGTTGACGATTACGAAAGCCTCCTCAAATATGCCAAACTAATTTCCCCTCATGATAAACTCTCCAATCACGTTAATGAACTCGTTCAAGGAATCATCGAAGGCGAGACACGTGTCCTCGTTGCTTCAATGACAATGGAAGAGGTTTTCAGAGGAACAAAAGAATTTAAACAAGAAGTATTCGACAAAGTTCAACTTGAATTGAACCAATTCGGATTATGGATTTACAATGCCAATGTTAAGCAATTGGTGGATGTGCCTGGTCATGAATATTTCTCATATTTGGGACAAAAGACGCAGATGGAAGCAGCGAATCAAGCTAGGGTTGACGTGGCAGAAGCTAAGATGAAAGGTGAAATTGGTTCGAAATTGAGGGAAGGACAAACGATCCAGAACGCAGCGAAAATTGATGCAGAGACGAAGGTTATTGCGATGCAGAGGGCTGGAGAAGGTGAGAAACAGGGAATTAAAGTGAGGACTGAAGTGAAAGTTTTTGAGAATCAAAGAGAGGCTGAAGTGGCTGAGGCTAATTCCGAGTTGGCTAAGAAGAAAGCTGCTTGGACAATGGCGGCTCAAGTGGCTGAGCTTGAGGCTGCTAAAGCCGTGGCTCTAAGGGAAGCTGAGTTGCAAGGTGAGGTTGAGAGGATGAATGCTTTGACCACTACTGAGAAGCTTAAAGCTGATTTCCTTAGCAAAGCTAGTGTTGAATATGACACTAAGGTTAGTCCTTGCAAATTTAACTTCTTCTTTCATCTGTTAATTATTATTCAAATTGTTTTACTAGCAACAAGAGTAATACTTCAATTAAGTCACTGTAATTACAGTTTTTTGGCATACATGTAATGAGACTATTATATATTGAGGTGTTAATGTTGAACTTTAAGTTTAAGCTCCTAGGTATATGTATGAATTTAACAGTATTTTACCCCCTTTGTGATTGGTTTCAATATACAATAGTCCTACGTCCGTGCACTGCATATTTACGAAGATCATAGGACGGGAGTTAAAAATTCAATGGCGCTATATAGTTGCCTTTTATTAAACTATATTGTGGTTGCAGATAGGTTTTGAATGTTTGTTTCTCAAAAGCTAGAGACCCCCAATCGACAGATTAAACATTACACAAAGAGAAGTAGCAAAATGCTAATTGAGGCCGCAAATCCATAGTTAATATACCTTGCAAATGAACATATATCATTTGCTAAATTTCTGTATCATTATAAAATTAAATCATTCGGTATGTTCATATAATAGTAGTATCATGACAGTTTTGTTAAGAACATGCCAAGTCTAAATCATGATCCTTTTCTTGCTATTATTTGTGTACTTATACTCAAGTTTGTTGAGTTAGCAAGTGCTATGGTTTGTTTGAATTAAGAAAAAGATTGAAGAATGTGGAGTTATCACTACCAACCTTGTCAATTTTTGTTAACAGGCAACCGTTGATTAAAAGTAAAACATAACAAGTAGGTTGTTTACTCGATCAATGATTTTCAATGTTCCACCACCAAAAAAAAGAAGATTTTTAATGTTTACTTTTTAAGTGCTGGTTGAAATTCTGGATGCTCTAGGTACAAGAGGCTAACTGGGAATTATACAAGAAACAAAAAGAGGCAGAAGCAATTCTGTATGAGAAGAAGGCAGAGGCAGAAGCGCAAAAAGCTTTGGCAGATTCCACATTTTATGCTCGCAAACAAGAAGCTGAAGCAGAGTTATACGCAAAGAAAAAAGAGGCAGAGGGGATTGTGACACTTGGGAATGCTCAAGGAGCATATGTAAGTACACTTCTCAATGCACTTGGGAACAACTACACAGCTGTAAGGGATTACTTGATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCCGAGGCGGTACGCGGACTTGAGCCTAAGATTAGTATTTGGACTAATGGTGGTGATAATAACGGGGGAATAACTGAAGGTGCCATGGGTATGAAAGAAGTTGCTGGTGTCTACAAGATGTTGCCACCTCTGTTTAAGACTGTCCATGAGCAAACAGGCATGTTGCCTCCGGCTTGGATGGGATCTTTACCTGACAAAAACTCTTAA
Protein sequence

>MS.gene32671.t1

MYRVAKASEYLVITGAGIDDVKLEKKAWIFPGQSCTVFDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDYESLLKYAKLISPHDKLSNHVNELVQGIIEGETRVLVASMTMEEVFRGTKEFKQEVFDKVQLELNQFGLWIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARVDVAEAKMKGEIGSKLREGQTIQNAAKIDAETKVIAMQRAGEGEKQGIKVRTEVKVFENQREAEVAEANSELAKKKAAWTMAAQVAELEAAKAVALREAELQGEVERMNALTTTEKLKADFLSKASVEYDTKVQEANWELYKKQKEAEAILYEKKAEAEAQKALADSTFYARKQEAEAELYAKKKEAEGIVTLGNAQGAYVSTLLNALGNNYTAVRDYLMINGGMFQEIAKINAEAVRGLEPKISIWTNGGDNNGGITEGAMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGSLPDKNS