AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52448


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52448.t1 MTR_0064s0160 69.082 207 60 3 30 232 238 444 1.73e-94 284
MS.gene52448.t1 MTR_1g061330 69.268 205 56 4 29 228 175 377 4.42e-92 276
MS.gene52448.t1 MTR_5g048160 57.576 198 66 7 29 210 196 391 1.31e-61 197
MS.gene52448.t1 MTR_0115s0020 56.784 199 67 8 29 210 202 398 9.69e-61 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGATATCTTCAGGTTTC+TGG 0.094666 7.2:-60630769 MS.gene52448:CDS
CATTTCTCTAATCCCTTTAC+AGG 0.173221 7.2:+60630964 None:intergenic
TGGTAACTTTATGAGGATTT+TGG 0.244298 7.2:+60631785 None:intergenic
TTTGGTTGATGAAAGATTAT+TGG 0.255893 7.2:-60630933 MS.gene52448:CDS
ATGGGAGTAATAGGTTTCTT+TGG 0.307329 7.2:-60630840 MS.gene52448:CDS
AATAAGAGCTGCTAGATATA+TGG 0.316809 7.2:-60630734 MS.gene52448:CDS
GAATTAGAATGCTCAATCTT+TGG 0.332900 7.2:+60631054 None:intergenic
CTATTGCTGGTAACTTTATG+AGG 0.343221 7.2:+60631778 None:intergenic
ACATGGTCGATGAACGGTTT+AGG 0.346638 7.2:-60631089 MS.gene52448:CDS
GTCATTGTTTATCCTGTAAA+GGG 0.361651 7.2:-60630976 MS.gene52448:CDS
CAGTGATTTATAAGCTTGTT+GGG 0.370830 7.2:-60630867 MS.gene52448:CDS
TAGAGAAATGGTTTGATGTT+TGG 0.375876 7.2:-60630951 MS.gene52448:CDS
TTGGTTGATGAAAGATTATT+GGG 0.379665 7.2:-60630932 MS.gene52448:CDS
CCAGTGATTTATAAGCTTGT+TGG 0.381944 7.2:-60630868 MS.gene52448:CDS
TTTGATGAATCTGATACTAT+TGG 0.385243 7.2:-60631000 MS.gene52448:CDS
TGGAAATATAAGATATCTTC+AGG 0.389661 7.2:-60630776 MS.gene52448:CDS
GCAAATCCATTTGCAAATCA+TGG 0.393739 7.2:-60631743 MS.gene52448:intron
CTGTAAAGGGATTAGAGAAA+TGG 0.411920 7.2:-60630963 MS.gene52448:CDS
ATGAAAGATTATTGGGATGA+AGG 0.426247 7.2:-60630925 MS.gene52448:CDS
TGTCATTGTTTATCCTGTAA+AGG 0.431563 7.2:-60630977 MS.gene52448:CDS
ATTATTGGGATGAAGGGTCT+TGG 0.438909 7.2:-60630918 MS.gene52448:CDS
TGGATCAAGTTATATAGTAT+TGG 0.438963 7.2:-60630898 MS.gene52448:CDS
TGCGAAATTGTTGTCATTGG+TGG 0.446619 7.2:-60631155 MS.gene52448:CDS
ATCTAACCATGATTTGCAAA+TGG 0.451488 7.2:+60631737 None:intergenic
GCGAAATTGTTGTCATTGGT+GGG 0.458301 7.2:-60631154 MS.gene52448:CDS
TTGTGCGAAATTGTTGTCAT+TGG 0.471678 7.2:-60631158 MS.gene52448:CDS
GAAAGATAGCAATGAGAGGT+TGG 0.477079 7.2:-60630818 MS.gene52448:CDS
ATAGTATCAGATTCATCAAA+AGG 0.488870 7.2:+60631003 None:intergenic
GAAGAACCCCAAATATCAAA+AGG 0.492126 7.2:+60631195 None:intergenic
ATTGAGCATTCTAATTCCTC+AGG 0.497942 7.2:-60631048 MS.gene52448:CDS
TGAAAGATTATTGGGATGAA+GGG 0.520538 7.2:-60630924 MS.gene52448:CDS
AAAAGGAAGAGGTAAATCTT+CGG 0.527250 7.2:+60631212 None:intergenic
CATTCGACATGGTCGATGAA+CGG 0.531410 7.2:-60631095 MS.gene52448:CDS
AGCTTATAAATCACTGGCAC+AGG 0.536304 7.2:+60630874 None:intergenic
TTGGTGTTGTATGAAGTTGA+GGG 0.539970 7.2:-60630799 MS.gene52448:CDS
CCAACAAGCTTATAAATCAC+TGG 0.548782 7.2:+60630868 None:intergenic
CGAAATTGTTGTCATTGGTG+GGG 0.549815 7.2:-60631153 MS.gene52448:CDS
CTTCAAAGAGGAAATGAAAG+TGG 0.549958 7.2:-60630697 MS.gene52448:CDS
GTTGGTGTTGTATGAAGTTG+AGG 0.558932 7.2:-60630800 MS.gene52448:CDS
CCCCAAATATCAAAAGGAAG+AGG 0.561584 7.2:+60631201 None:intergenic
AGTCTTGTTTCACTTCAAAG+AGG 0.582727 7.2:-60630709 MS.gene52448:CDS
GTGTTGTATGAAGTTGAGGG+TGG 0.632253 7.2:-60630796 MS.gene52448:CDS
TTTGGAAAGATAGCAATGAG+AGG 0.639164 7.2:-60630822 MS.gene52448:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTAGTAATAAATTG+AGG + chr7.2:60630753-60630772 None:intergenic 15.0%
!!! ACTTTATAATCATTAGTTTT+AGG + chr7.2:60631113-60631132 None:intergenic 15.0%
!!! AGAAGAAATAATATGTTTTT+TGG + chr7.2:60630880-60630899 None:intergenic 15.0%
!!! ATAGTTTTATTTCAAATTCT+TGG - chr7.2:60631204-60631223 MS.gene52448:CDS 15.0%
!!! CTTTATAATCATTAGTTTTA+GGG + chr7.2:60631112-60631131 None:intergenic 15.0%
!!! GAAGAAATAATATGTTTTTT+GGG + chr7.2:60630879-60630898 None:intergenic 15.0%
!!! TATTCTTTTAGTGAATATTA+TGG - chr7.2:60631074-60631093 MS.gene52448:CDS 15.0%
!! AAATATTGGACTGAAATAAT+GGG - chr7.2:60630939-60630958 MS.gene52448:CDS 20.0%
!! TAAATATTGGACTGAAATAA+TGG - chr7.2:60630938-60630957 MS.gene52448:CDS 20.0%
!! TTATTTCAGTCCAATATTTA+TGG + chr7.2:60630938-60630957 None:intergenic 20.0%
!!! TTTAGTGAATATTATGGTTT+TGG - chr7.2:60631080-60631099 MS.gene52448:CDS 20.0%
! ACAATTCTGACCATAAATAT+TGG - chr7.2:60630925-60630944 MS.gene52448:CDS 25.0%
! ATAGTATCAGATTCATCAAA+AGG + chr7.2:60631452-60631471 None:intergenic 25.0%
! CTAAAAGAATAAAAACCTTG+AGG + chr7.2:60631065-60631084 None:intergenic 25.0%
! TGGAAATATAAGATATCTTC+AGG - chr7.2:60631676-60631695 MS.gene52448:intron 25.0%
! TGGATCAAGTTATATAGTAT+TGG - chr7.2:60631554-60631573 MS.gene52448:intron 25.0%
! TTTGATGAATCTGATACTAT+TGG - chr7.2:60631452-60631471 MS.gene52448:intron 25.0%
!! ATTTTGGATACTAAATCTTG+TGG + chr7.2:60630654-60630673 None:intergenic 25.0%
!! TCAATGTTTTTCTTGTAGTT+TGG - chr7.2:60631781-60631800 MS.gene52448:CDS 25.0%
!!! GTTTTATTTCAAATTCTTGG+AGG - chr7.2:60631207-60631226 MS.gene52448:CDS 25.0%
!!! TTGGTTGATGAAAGATTATT+GGG - chr7.2:60631520-60631539 MS.gene52448:intron 25.0%
!!! TTTGGTTGATGAAAGATTAT+TGG - chr7.2:60631519-60631538 MS.gene52448:intron 25.0%
AAAAGGAAGAGGTAAATCTT+CGG + chr7.2:60631243-60631262 None:intergenic 30.0%
AAACAGATGAGAGACAAAAT+GGG - chr7.2:60631162-60631181 MS.gene52448:CDS 30.0%
AATAAGAGCTGCTAGATATA+TGG - chr7.2:60631718-60631737 MS.gene52448:intron 30.0%
AATGGGATATACTTTCTAGA+AGG - chr7.2:60630969-60630988 MS.gene52448:CDS 30.0%
ACCATAAACTGTATCAAAGA+TGG + chr7.2:60631017-60631036 None:intergenic 30.0%
ATGAAAGATTATTGGGATGA+AGG - chr7.2:60631527-60631546 MS.gene52448:intron 30.0%
CAGAATTGTATTCATAAGGT+GGG + chr7.2:60630914-60630933 None:intergenic 30.0%
CTTACATTTCACATCTTTCT+TGG - chr7.2:60630841-60630860 MS.gene52448:CDS 30.0%
GAATTAGAATGCTCAATCTT+TGG + chr7.2:60631401-60631420 None:intergenic 30.0%
GTCATTGTTTATCCTGTAAA+GGG - chr7.2:60631476-60631495 MS.gene52448:intron 30.0%
GTTGAAAGATCTTTGGTTAA+AGG - chr7.2:60631139-60631158 MS.gene52448:CDS 30.0%
TAGAGAAATGGTTTGATGTT+TGG - chr7.2:60631501-60631520 MS.gene52448:intron 30.0%
TCAGAATTGTATTCATAAGG+TGG + chr7.2:60630915-60630934 None:intergenic 30.0%
TCCATCTTTGATACAGTTTA+TGG - chr7.2:60631013-60631032 MS.gene52448:CDS 30.0%
TGAAAGATTATTGGGATGAA+GGG - chr7.2:60631528-60631547 MS.gene52448:intron 30.0%
TGACTAAAAGATGATCTTGT+TGG + chr7.2:60630791-60630810 None:intergenic 30.0%
TGGTCAGAATTGTATTCATA+AGG + chr7.2:60630918-60630937 None:intergenic 30.0%
TGTCATTGTTTATCCTGTAA+AGG - chr7.2:60631475-60631494 MS.gene52448:intron 30.0%
TTGTTGTGTTGAAAGATCTT+TGG - chr7.2:60631132-60631151 MS.gene52448:CDS 30.0%
! ACCTCTTCCTTTTGATATTT+GGG - chr7.2:60631250-60631269 MS.gene52448:intron 30.0%
! AGAAAGTATATCCCATTACA+AGG + chr7.2:60630966-60630985 None:intergenic 30.0%
! ATAAGATATCTTCAGGTTTC+TGG - chr7.2:60631683-60631702 MS.gene52448:intron 30.0%
! ATCTAACCATGATTTGCAAA+TGG + chr7.2:60630718-60630737 None:intergenic 30.0%
! CAGTGATTTATAAGCTTGTT+GGG - chr7.2:60631585-60631604 MS.gene52448:intron 30.0%
! GATGGATTCATCATTACATT+TGG + chr7.2:60630999-60631018 None:intergenic 30.0%
! TACCTCTTCCTTTTGATATT+TGG - chr7.2:60631249-60631268 MS.gene52448:intron 30.0%
! TGGTAACTTTATGAGGATTT+TGG + chr7.2:60630670-60630689 None:intergenic 30.0%
!! AAATAATGGGTCCTTGTAAT+GGG - chr7.2:60630952-60630971 MS.gene52448:CDS 30.0%
!! CATTTTGATAACTCTATTGC+TGG + chr7.2:60630690-60630709 None:intergenic 30.0%
!!! ATAAGCTTGTTGGGTTTTAT+GGG - chr7.2:60631594-60631613 MS.gene52448:intron 30.0%
!!! GGTTTTGTTGATGGAAATAT+AGG - chr7.2:60631320-60631339 MS.gene52448:intron 30.0%
!!! TATAAGCTTGTTGGGTTTTA+TGG - chr7.2:60631593-60631612 MS.gene52448:intron 30.0%
!!! TGTTTTAAAACATTCACCTG+AGG + chr7.2:60631423-60631442 None:intergenic 30.0%
ATGAGAGACAAAATGGGTAT+TGG - chr7.2:60631168-60631187 MS.gene52448:CDS 35.0%
ATGGGAGTAATAGGTTTCTT+TGG - chr7.2:60631612-60631631 MS.gene52448:intron 35.0%
ATTGAGCATTCTAATTCCTC+AGG - chr7.2:60631404-60631423 MS.gene52448:intron 35.0%
CATTTCTCTAATCCCTTTAC+AGG + chr7.2:60631491-60631510 None:intergenic 35.0%
CCAACAAGCTTATAAATCAC+TGG + chr7.2:60631587-60631606 None:intergenic 35.0%
CCAGTGATTTATAAGCTTGT+TGG - chr7.2:60631584-60631603 MS.gene52448:intron 35.0%
CTATTGCTGGTAACTTTATG+AGG + chr7.2:60630677-60630696 None:intergenic 35.0%
CTGTAAAGGGATTAGAGAAA+TGG - chr7.2:60631489-60631508 MS.gene52448:intron 35.0%
CTTCAAAGAGGAAATGAAAG+TGG - chr7.2:60631755-60631774 MS.gene52448:CDS 35.0%
GAAACAGATGAGAGACAAAA+TGG - chr7.2:60631161-60631180 MS.gene52448:CDS 35.0%
GAAGAACCCCAAATATCAAA+AGG + chr7.2:60631260-60631279 None:intergenic 35.0%
GCAAATCCATTTGCAAATCA+TGG - chr7.2:60630709-60630728 MS.gene52448:CDS 35.0%
TTGTGCGAAATTGTTGTCAT+TGG - chr7.2:60631294-60631313 MS.gene52448:intron 35.0%
TTTGGAAAGATAGCAATGAG+AGG - chr7.2:60631630-60631649 MS.gene52448:intron 35.0%
! ATTCGTAGACCTTTTCTTTG+TGG - chr7.2:60630810-60630829 MS.gene52448:CDS 35.0%
! CCTCTTCCTTTTGATATTTG+GGG - chr7.2:60631251-60631270 MS.gene52448:intron 35.0%
!! AGTCTTGTTTCACTTCAAAG+AGG - chr7.2:60631743-60631762 MS.gene52448:intron 35.0%
!! GAAATAATGGGTCCTTGTAA+TGG - chr7.2:60630951-60630970 MS.gene52448:CDS 35.0%
!! TTGGGTTTTATGGGAGTAAT+AGG - chr7.2:60631603-60631622 MS.gene52448:intron 35.0%
!! TTGGTGTTGTATGAAGTTGA+GGG - chr7.2:60631653-60631672 MS.gene52448:intron 35.0%
!!! GTTTTGAGACCACAAAGAAA+AGG + chr7.2:60630822-60630841 None:intergenic 35.0%
!!! TGTTGTTTTAGCATTCGACA+TGG - chr7.2:60631346-60631365 MS.gene52448:intron 35.0%
AACCTTGAGGAGCTGTAAAA+TGG + chr7.2:60631052-60631071 None:intergenic 40.0%
ACCTTGAGGAGCTGTAAAAT+GGG + chr7.2:60631051-60631070 None:intergenic 40.0%
AGCTTATAAATCACTGGCAC+AGG + chr7.2:60631581-60631600 None:intergenic 40.0%
ATTATTGGGATGAAGGGTCT+TGG - chr7.2:60631534-60631553 MS.gene52448:intron 40.0%
CCCCAAATATCAAAAGGAAG+AGG + chr7.2:60631254-60631273 None:intergenic 40.0%
CGAAATTGTTGTCATTGGTG+GGG - chr7.2:60631299-60631318 MS.gene52448:intron 40.0%
GAAAGATAGCAATGAGAGGT+TGG - chr7.2:60631634-60631653 MS.gene52448:intron 40.0%
GCGAAATTGTTGTCATTGGT+GGG - chr7.2:60631298-60631317 MS.gene52448:intron 40.0%
TGCGAAATTGTTGTCATTGG+TGG - chr7.2:60631297-60631316 MS.gene52448:intron 40.0%
! ATGATCCTCTTTTGAACCTG+GGG - chr7.2:60631807-60631826 MS.gene52448:CDS 40.0%
! CATGATCCTCTTTTGAACCT+GGG - chr7.2:60631806-60631825 MS.gene52448:CDS 40.0%
! TCATGATCCTCTTTTGAACC+TGG - chr7.2:60631805-60631824 MS.gene52448:CDS 40.0%
!! GTTGGTGTTGTATGAAGTTG+AGG - chr7.2:60631652-60631671 MS.gene52448:intron 40.0%
ACATGGTCGATGAACGGTTT+AGG - chr7.2:60631363-60631382 MS.gene52448:intron 45.0%
CATTCGACATGGTCGATGAA+CGG - chr7.2:60631357-60631376 MS.gene52448:intron 45.0%
GGAGCTGTAAAATGGGATTG+AGG + chr7.2:60631044-60631063 None:intergenic 45.0%
GTGTTGTATGAAGTTGAGGG+TGG - chr7.2:60631656-60631675 MS.gene52448:intron 45.0%
! TCCCATTTTACAGCTCCTCA+AGG - chr7.2:60631047-60631066 MS.gene52448:CDS 45.0%
! TGATCCTCTTTTGAACCTGG+GGG - chr7.2:60631808-60631827 MS.gene52448:CDS 45.0%
!!! CATTGGTGGGGTTTTGTTGA+TGG - chr7.2:60631311-60631330 MS.gene52448:intron 45.0%
TACTCCCCCAGGTTCAAAAG+AGG + chr7.2:60631815-60631834 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 60630639 60631835 60630639 ID=MS.gene52448
chr7.2 mRNA 60630639 60631835 60630639 ID=MS.gene52448.t1;Parent=MS.gene52448
chr7.2 exon 60631744 60631835 60631744 ID=MS.gene52448.t1.exon1;Parent=MS.gene52448.t1
chr7.2 CDS 60631744 60631835 60631744 ID=cds.MS.gene52448.t1;Parent=MS.gene52448.t1
chr7.2 exon 60630639 60631245 60630639 ID=MS.gene52448.t1.exon2;Parent=MS.gene52448.t1
chr7.2 CDS 60630639 60631245 60630639 ID=cds.MS.gene52448.t1;Parent=MS.gene52448.t1
Gene Sequence

>MS.gene52448

ATGGAGCGTTTGCCACAAGATTTAGTATCCAAAATCCTCATAAAGTTACCAGCAATAGAGTTATCAAAATGCAAATCCATTTGCAAATCATGGTTAGATCTCATAACTAATCCTCAATTTATTACTAATTACTATATTATCTACAACAACCAACAAGATCATCTTTTAGTCATTCGTAGACCTTTTCTTTGTGGTCTCAAAACTTACATTTCACATCTTTCTTGGAATTTTAATGATCCCAAAAAACATATTATTTCTTCTGAGATTTTTAACCCACCTTATGAATACAATTCTGACCATAAATATTGGACTGAAATAATGGGTCCTTGTAATGGGATATACTTTCTAGAAGGTAATCCAAATGTAATGATGAATCCATCTTTGATACAGTTTATGGTTTTGCCTCAATCCCATTTTACAGCTCCTCAAGGTTTTTATTCTTTTAGTGAATATTATGGTTTTGGTTTTGACCCTAAAACTAATGATTATAAAGTTGTTGTGTTGAAAGATCTTTGGTTAAAGGAAACAGATGAGAGACAAAATGGGTATTGGAATGTTGAGTTATATAGTTTTATTTCAAATTCTTGGAGGAAACTTGATGCCGAAGATTTACCTCTTCCTTTTGATATTTGGGGTTCTTCTAGAATCTACACTTTTGTGCGAAATTGTTGTCATTGGTGGGGTTTTGTTGATGGAAATATAGGAGATGTTGTTTTAGCATTCGACATGGTCGATGAACGGTTTAGGAAGATAAAAGTGCCAAAGATTGAGCATTCTAATTCCTCAGGTGAATGTTTTAAAACATTAGTACCTTTTGATGAATCTGATACTATTGGTGTCATTGTTTATCCTGTAAAGGGATTAGAGAAATGGTTTGATGTTTGGTTGATGAAAGATTATTGGGATGAAGGGTCTTGGATCAAGTTATATAGTATTGGACCTGTGCCAGTGATTTATAAGCTTGTTGGGTTTTATGGGAGTAATAGGTTTCTTTGGAAAGATAGCAATGAGAGGTTGGTGTTGTATGAAGTTGAGGGTGGAAATATAAGATATCTTCAGGTTTCTGGAAAGTATGATTCAATAAGAGCTGCTAGATATATGGAAAGTCTTGTTTCACTTCAAAGAGGAAATGAAAGTGGTCATCAATGTTTTTCTTGTAGTTTGGTTCATGATCCTCTTTTGAACCTGGGGGAGTAG

Protein sequence

>MS.gene52448.t1

MERLPQDLVSKILIKLPAIELSKCKSICKSWKLDAEDLPLPFDIWGSSRIYTFVRNCCHWWGFVDGNIGDVVLAFDMVDERFRKIKVPKIEHSNSSGECFKTLVPFDESDTIGVIVYPVKGLEKWFDVWLMKDYWDEGSWIKLYSIGPVPVIYKLVGFYGSNRFLWKDSNERLVLYEVEGGNIRYLQVSGKYDSIRAARYMESLVSLQRGNESGHQCFSCSLVHDPLLNLGE