AlfalfaGEDB | MS.gene20184

M. sativa cultivar XinJiangDaYe / MS.gene20184

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20184.t1	AET05080.1	93.7	126	8	0	1	126	169	294	2.10E-60	241.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20184.t1	G7LIV2	93.7	126	8	0	1	126	169	294	1.5e-60	241.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene20184	0.833182	6.18E-56	-1.69E-46
MS.gene049151	MS.gene20184	0.820186	7.84E-53	-1.69E-46
MS.gene049153	MS.gene20184	0.830485	2.86E-55	-1.69E-46
MS.gene049159	MS.gene20184	0.83777	4.27E-57	-1.69E-46
MS.gene049230	MS.gene20184	0.843786	1.13E-58	-1.69E-46
MS.gene049378	MS.gene20184	0.865934	4.12E-65	-1.69E-46
MS.gene049435	MS.gene20184	0.834513	2.87E-56	-1.69E-46
MS.gene049443	MS.gene20184	0.81808	2.36E-52	-1.69E-46
MS.gene049629	MS.gene20184	0.812185	4.81E-51	-1.69E-46
MS.gene050226	MS.gene20184	0.859481	4.02E-63	-1.69E-46
MS.gene050227	MS.gene20184	0.85601	4.29E-62	-1.69E-46
MS.gene050228	MS.gene20184	0.854195	1.45E-61	-1.69E-46
MS.gene050442	MS.gene20184	0.856928	2.31E-62	-1.69E-46
MS.gene050605	MS.gene20184	0.833939	4.00E-56	-1.69E-46
MS.gene050623	MS.gene20184	-0.871192	8.24E-67	-1.69E-46
MS.gene050642	MS.gene20184	0.83781	4.17E-57	-1.69E-46
MS.gene050680	MS.gene20184	0.840269	9.62E-58	-1.69E-46
MS.gene050719	MS.gene20184	0.822809	1.94E-53	-1.69E-46
MS.gene050721	MS.gene20184	0.818612	1.79E-52	-1.69E-46
MS.gene050724	MS.gene20184	0.833682	4.63E-56	-1.69E-46
MS.gene050750	MS.gene20184	0.814012	1.91E-51	-1.69E-46
MS.gene050762	MS.gene20184	0.80527	1.45E-49	-1.69E-46
MS.gene050763	MS.gene20184	0.807857	4.12E-50	-1.69E-46
MS.gene050820	MS.gene20184	0.825854	3.74E-54	-1.69E-46
MS.gene050824	MS.gene20184	0.824674	7.10E-54	-1.69E-46
MS.gene050826	MS.gene20184	0.804298	2.31E-49	-1.69E-46
MS.gene050953	MS.gene20184	0.820023	8.54E-53	-1.69E-46
MS.gene051210	MS.gene20184	0.800247	1.58E-48	-1.69E-46
MS.gene051344	MS.gene20184	0.808814	2.57E-50	-1.69E-46
MS.gene051370	MS.gene20184	0.802169	6.37E-49	-1.69E-46
MS.gene051860	MS.gene20184	0.839466	1.56E-57	-1.69E-46
MS.gene051983	MS.gene20184	0.865775	4.62E-65	-1.69E-46
MS.gene052012	MS.gene20184	0.813781	2.15E-51	-1.69E-46
MS.gene052176	MS.gene20184	0.830511	2.82E-55	-1.69E-46
MS.gene052190	MS.gene20184	0.80896	2.40E-50	-1.69E-46
MS.gene052309	MS.gene20184	-0.800817	1.21E-48	-1.69E-46
MS.gene052323	MS.gene20184	0.806821	6.83E-50	-1.69E-46
MS.gene052333	MS.gene20184	0.839933	1.18E-57	-1.69E-46
MS.gene052389	MS.gene20184	0.817985	2.48E-52	-1.69E-46
MS.gene052426	MS.gene20184	0.837312	5.60E-57	-1.69E-46
MS.gene052863	MS.gene20184	0.805799	1.12E-49	-1.69E-46
MS.gene053008	MS.gene20184	0.825943	3.56E-54	-1.69E-46
MS.gene053166	MS.gene20184	0.82877	7.47E-55	-1.69E-46
MS.gene053216	MS.gene20184	0.816542	5.24E-52	-1.69E-46
MS.gene053494	MS.gene20184	0.824623	7.30E-54	-1.69E-46
MS.gene053584	MS.gene20184	0.80814	3.58E-50	-1.69E-46
MS.gene053836	MS.gene20184	0.816599	5.09E-52	-1.69E-46
MS.gene05415	MS.gene20184	0.801958	7.04E-49	-1.69E-46
MS.gene054184	MS.gene20184	0.804282	2.33E-49	-1.69E-46
MS.gene054185	MS.gene20184	0.802183	6.33E-49	-1.69E-46
MS.gene054236	MS.gene20184	0.830424	2.96E-55	-1.69E-46
MS.gene054291	MS.gene20184	-0.804195	2.43E-49	-1.69E-46
MS.gene054379	MS.gene20184	0.846193	2.53E-59	-1.69E-46
MS.gene054405	MS.gene20184	0.81655	5.22E-52	-1.69E-46
MS.gene054467	MS.gene20184	0.82243	2.38E-53	-1.69E-46
MS.gene054468	MS.gene20184	0.835536	1.59E-56	-1.69E-46
MS.gene054479	MS.gene20184	0.801481	8.82E-49	-1.69E-46
MS.gene054559	MS.gene20184	0.838879	2.21E-57	-1.69E-46
MS.gene054657	MS.gene20184	0.807861	4.11E-50	-1.69E-46
MS.gene054658	MS.gene20184	0.828676	7.88E-55	-1.69E-46
MS.gene054930	MS.gene20184	0.851349	9.41E-61	-1.69E-46
MS.gene05510	MS.gene20184	0.852141	5.61E-61	-1.69E-46
MS.gene055222	MS.gene20184	0.809472	1.86E-50	-1.69E-46
MS.gene055227	MS.gene20184	0.808823	2.56E-50	-1.69E-46
MS.gene055356	MS.gene20184	0.831605	1.52E-55	-1.69E-46
MS.gene055358	MS.gene20184	0.814022	1.90E-51	-1.69E-46
MS.gene055623	MS.gene20184	0.811978	5.34E-51	-1.69E-46
MS.gene055701	MS.gene20184	0.877553	5.73E-69	-1.69E-46
MS.gene055818	MS.gene20184	0.812285	4.58E-51	-1.69E-46
MS.gene055926	MS.gene20184	0.812515	4.08E-51	-1.69E-46
MS.gene05595	MS.gene20184	0.818892	1.55E-52	-1.69E-46
MS.gene05604	MS.gene20184	0.800276	1.55E-48	-1.69E-46
MS.gene056173	MS.gene20184	0.805403	1.36E-49	-1.69E-46
MS.gene056447	MS.gene20184	0.8102	1.30E-50	-1.69E-46
MS.gene056506	MS.gene20184	0.853898	1.76E-61	-1.69E-46
MS.gene056873	MS.gene20184	0.811344	7.34E-51	-1.69E-46
MS.gene056979	MS.gene20184	0.862238	5.84E-64	-1.69E-46
MS.gene057026	MS.gene20184	0.817093	3.94E-52	-1.69E-46
MS.gene057060	MS.gene20184	0.802405	5.70E-49	-1.69E-46
MS.gene057194	MS.gene20184	0.817924	2.56E-52	-1.69E-46
MS.gene057200	MS.gene20184	0.806567	7.73E-50	-1.69E-46
MS.gene057394	MS.gene20184	0.807265	5.50E-50	-1.69E-46
MS.gene057469	MS.gene20184	0.803009	4.28E-49	-1.69E-46
MS.gene05761	MS.gene20184	0.821133	4.75E-53	-1.69E-46
MS.gene057750	MS.gene20184	0.812746	3.63E-51	-1.69E-46
MS.gene057869	MS.gene20184	0.815374	9.55E-52	-1.69E-46
MS.gene058139	MS.gene20184	0.818478	1.92E-52	-1.69E-46
MS.gene058146	MS.gene20184	0.851266	9.92E-61	-1.69E-46
MS.gene058399	MS.gene20184	0.861628	8.98E-64	-1.69E-46
MS.gene058435	MS.gene20184	0.807415	5.11E-50	-1.69E-46
MS.gene058548	MS.gene20184	0.811978	5.34E-51	-1.69E-46
MS.gene058549	MS.gene20184	0.807464	4.99E-50	-1.69E-46
MS.gene058595	MS.gene20184	0.836073	1.16E-56	-1.69E-46
MS.gene058710	MS.gene20184	0.802335	5.89E-49	-1.69E-46
MS.gene058979	MS.gene20184	0.811201	7.88E-51	-1.69E-46
MS.gene059124	MS.gene20184	0.815555	8.71E-52	-1.69E-46
MS.gene059128	MS.gene20184	0.836128	1.12E-56	-1.69E-46
MS.gene059161	MS.gene20184	0.838768	2.36E-57	-1.69E-46
MS.gene059382	MS.gene20184	0.81347	2.52E-51	-1.69E-46
MS.gene059609	MS.gene20184	0.834416	3.04E-56	-1.69E-46
MS.gene059789	MS.gene20184	0.822184	2.71E-53	-1.69E-46
MS.gene05979	MS.gene20184	0.822308	2.54E-53	-1.69E-46
MS.gene05984	MS.gene20184	0.80187	7.34E-49	-1.69E-46
MS.gene060011	MS.gene20184	0.819043	1.43E-52	-1.69E-46
MS.gene060084	MS.gene20184	0.801613	8.29E-49	-1.69E-46
MS.gene060137	MS.gene20184	-0.81089	9.20E-51	-1.69E-46
MS.gene060322	MS.gene20184	0.829409	5.23E-55	-1.69E-46
MS.gene060572	MS.gene20184	0.803739	3.02E-49	-1.69E-46
MS.gene060683	MS.gene20184	0.855665	5.42E-62	-1.69E-46
MS.gene060685	MS.gene20184	0.853827	1.85E-61	-1.69E-46
MS.gene060802	MS.gene20184	0.847317	1.25E-59	-1.69E-46
MS.gene060803	MS.gene20184	0.857811	1.27E-62	-1.69E-46
MS.gene060942	MS.gene20184	0.820122	8.10E-53	-1.69E-46
MS.gene060998	MS.gene20184	0.85159	8.04E-61	-1.69E-46
MS.gene061002	MS.gene20184	0.800755	1.24E-48	-1.69E-46
MS.gene061040	MS.gene20184	-0.809956	1.46E-50	-1.69E-46
MS.gene061114	MS.gene20184	-0.806601	7.60E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20184.t1	MTR_8g101290	93.651	126	8	0	1	126	169	294	9.64e-83	244

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20184.t1	AT2G37400	38.739	111	64	2	17	126	161	268	2.49e-11	59.7

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTAGCTGCAGTGAGAATT+TGG	0.222303	4.4:-4142817	MS.gene20184:CDS
AATCGTTATCTTAGATTATT+CGG	0.255975	4.4:-4142627	MS.gene20184:CDS
GCAAGGTTGTTGATCGTATT+AGG	0.259715	4.4:-4142605	MS.gene20184:CDS
TGCCTTGTTGAAGGGTATTA+AGG	0.259824	4.4:-4142688	MS.gene20184:CDS
AGAGATATTGGAGAAAGATA+TGG	0.380622	4.4:-4142772	MS.gene20184:CDS
TAGGGTCTTGGAAGGAGATA+TGG	0.385995	4.4:-4142586	MS.gene20184:CDS
TGTTGATCGTATTAGGGTCT+TGG	0.393710	4.4:-4142598	MS.gene20184:CDS
CAAGGTTGTTGATCGTATTA+GGG	0.400610	4.4:-4142604	MS.gene20184:CDS
AATTTGGAGTTGATTAAGAT+TGG	0.405158	4.4:-4142801	MS.gene20184:CDS
AACATTCATCAACCATTTCA+AGG	0.417026	4.4:+4142712	None:intergenic
TGTTCTCGAGTACCTTGAAA+TGG	0.474209	4.4:-4142724	MS.gene20184:CDS
AACTGATGATGATGAAGCAT+GGG	0.561928	4.4:-4142859	MS.gene20184:CDS
AATGGATAAGTGTGAGAGAG+AGG	0.561975	4.4:-4142661	MS.gene20184:CDS
GAAGGGTATTAAGGTTGCAA+TGG	0.563079	4.4:-4142679	MS.gene20184:CDS
TGAATGTTATGCCTTGTTGA+AGG	0.563253	4.4:-4142697	MS.gene20184:CDS
GATCGTATTAGGGTCTTGGA+AGG	0.579181	4.4:-4142594	MS.gene20184:CDS
GGATTGTAACAAGGCGCATG+AGG	0.585808	4.4:-4142751	MS.gene20184:CDS
GAATGTTATGCCTTGTTGAA+GGG	0.607528	4.4:-4142696	MS.gene20184:CDS
TTATCTTAGATTATTCGGCA+AGG	0.617181	4.4:-4142622	MS.gene20184:CDS
AAACTGATGATGATGAAGCA+TGG	0.620574	4.4:-4142860	MS.gene20184:CDS
GTCTTGGAAGGAGATATGGT+TGG	0.652336	4.4:-4142582	MS.gene20184:CDS
GAAAGATATGGATTGTAACA+AGG	0.667587	4.4:-4142760	MS.gene20184:CDS
AACCTTAATACCCTTCAACA+AGG	0.669269	4.4:+4142686	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATCGTTATCTTAGATTATT+CGG	-	chr4.4:4142821-4142840	MS.gene20184:CDS	20.0%
!!	AATTTGGAGTTGATTAAGAT+TGG	-	chr4.4:4142647-4142666	MS.gene20184:CDS	25.0%
	AACATTCATCAACCATTTCA+AGG	+	chr4.4:4142739-4142758	None:intergenic	30.0%
	AGAGATATTGGAGAAAGATA+TGG	-	chr4.4:4142676-4142695	MS.gene20184:CDS	30.0%
	TTATCTTAGATTATTCGGCA+AGG	-	chr4.4:4142826-4142845	MS.gene20184:CDS	30.0%
!	GAAAGATATGGATTGTAACA+AGG	-	chr4.4:4142688-4142707	MS.gene20184:CDS	30.0%
!!!	GATTGGTTTTGAAGAGATAT+TGG	-	chr4.4:4142664-4142683	MS.gene20184:CDS	30.0%
	AACCTTAATACCCTTCAACA+AGG	+	chr4.4:4142765-4142784	None:intergenic	35.0%
	CAAGGTTGTTGATCGTATTA+GGG	-	chr4.4:4142844-4142863	MS.gene20184:CDS	35.0%
!	AAACTGATGATGATGAAGCA+TGG	-	chr4.4:4142588-4142607	MS.gene20184:CDS	35.0%
!	AACTGATGATGATGAAGCAT+GGG	-	chr4.4:4142589-4142608	MS.gene20184:CDS	35.0%
!	GAATGTTATGCCTTGTTGAA+GGG	-	chr4.4:4142752-4142771	MS.gene20184:CDS	35.0%
!	TGAATGTTATGCCTTGTTGA+AGG	-	chr4.4:4142751-4142770	MS.gene20184:CDS	35.0%
	AATGGATAAGTGTGAGAGAG+AGG	-	chr4.4:4142787-4142806	MS.gene20184:CDS	40.0%
	GCAAGGTTGTTGATCGTATT+AGG	-	chr4.4:4142843-4142862	MS.gene20184:CDS	40.0%
	TGTTAGCTGCAGTGAGAATT+TGG	-	chr4.4:4142631-4142650	MS.gene20184:CDS	40.0%
	TGTTCTCGAGTACCTTGAAA+TGG	-	chr4.4:4142724-4142743	MS.gene20184:CDS	40.0%
	TGTTGATCGTATTAGGGTCT+TGG	-	chr4.4:4142850-4142869	MS.gene20184:CDS	40.0%
!!	GAAGGGTATTAAGGTTGCAA+TGG	-	chr4.4:4142769-4142788	MS.gene20184:CDS	40.0%
!!	TGCCTTGTTGAAGGGTATTA+AGG	-	chr4.4:4142760-4142779	MS.gene20184:CDS	40.0%
	GATCGTATTAGGGTCTTGGA+AGG	-	chr4.4:4142854-4142873	MS.gene20184:CDS	45.0%
	GTCTTGGAAGGAGATATGGT+TGG	-	chr4.4:4142866-4142885	MS.gene20184:CDS	45.0%
	TAGGGTCTTGGAAGGAGATA+TGG	-	chr4.4:4142862-4142881	MS.gene20184:CDS	45.0%
!!!	ATGGGATGTTTTGAGAGCAG+AGG	-	chr4.4:4142607-4142626	MS.gene20184:CDS	45.0%
	GGATTGTAACAAGGCGCATG+AGG	-	chr4.4:4142697-4142716	MS.gene20184:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	4142545	4142925	4142545	ID=MS.gene20184
chr4.4	mRNA	4142545	4142925	4142545	ID=MS.gene20184.t1;Parent=MS.gene20184
chr4.4	exon	4142545	4142925	4142545	ID=MS.gene20184.t1.exon1;Parent=MS.gene20184.t1
chr4.4	CDS	4142545	4142925	4142545	ID=cds.MS.gene20184.t1;Parent=MS.gene20184.t1

Gene Sequence

>MS.gene20184

ATGAAAGAGAGAATTCGCGTTGTTCACAGTTTCAAGAAAATTAAAACTGATGATGATGAAGCATGGGATGTTTTGAGAGCAGAGGTTGTTAGCTGCAGTGAGAATTTGGAGTTGATTAAGATTGGTTTTGAAGAGATATTGGAGAAAGATATGGATTGTAACAAGGCGCATGAGGATCGTGTTCTCGAGTACCTTGAAATGGTTGATGAATGTTATGCCTTGTTGAAGGGTATTAAGGTTGCAATGGATAAGTGTGAGAGAGAGGATGCAGATATAAATCGTTATCTTAGATTATTCGGCAAGGTTGTTGATCGTATTAGGGTCTTGGAAGGAGATATGGTTGGTGCTTTGAAGTATTTTAAGCAGCTTGAACAAGAGTGA

Protein sequence

>MS.gene20184.t1

MKERIRVVHSFKKIKTDDDEAWDVLRAEVVSCSENLELIKIGFEEILEKDMDCNKAHEDRVLEYLEMVDECYALLKGIKVAMDKCEREDADINRYLRLFGKVVDRIRVLEGDMVGALKYFKQLEQE