Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56431.t1 | XP_003607876.1 | 98.1 | 161 | 3 | 0 | 1 | 161 | 130 | 290 | 2.80E-89 | 337.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56431.t1 | O81865 | 51.8 | 164 | 76 | 2 | 1 | 162 | 84 | 246 | 9.1e-49 | 194.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56431.t1 | G7JIT3 | 98.1 | 161 | 3 | 0 | 1 | 161 | 130 | 290 | 2.0e-89 | 337.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene56431 | 0.830843 | 2.34E-55 | -1.69E-46 |
| MS.gene049151 | MS.gene56431 | 0.800149 | 1.65E-48 | -1.69E-46 |
| MS.gene049153 | MS.gene56431 | 0.812304 | 4.53E-51 | -1.69E-46 |
| MS.gene049159 | MS.gene56431 | 0.863623 | 2.18E-64 | -1.69E-46 |
| MS.gene049230 | MS.gene56431 | 0.807126 | 5.89E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene56431 | 0.842427 | 2.60E-58 | -1.69E-46 |
| MS.gene049424 | MS.gene56431 | 0.809028 | 2.32E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene56431 | 0.820966 | 5.19E-53 | -1.69E-46 |
| MS.gene049669 | MS.gene56431 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene050105 | MS.gene56431 | 0.813227 | 2.85E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene56431 | 0.822269 | 2.59E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene56431 | 0.834456 | 2.97E-56 | -1.69E-46 |
| MS.gene050228 | MS.gene56431 | 0.827219 | 1.76E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene56431 | -0.807772 | 4.29E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene56431 | 0.835608 | 1.52E-56 | -1.69E-46 |
| MS.gene050605 | MS.gene56431 | 0.822038 | 2.93E-53 | -1.69E-46 |
| MS.gene050623 | MS.gene56431 | -0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene050680 | MS.gene56431 | 0.878134 | 3.59E-69 | -1.69E-46 |
| MS.gene050719 | MS.gene56431 | 0.80484 | 1.78E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene56431 | 0.80284 | 4.64E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene56431 | 0.823903 | 1.08E-53 | -1.69E-46 |
| MS.gene050750 | MS.gene56431 | 0.850356 | 1.79E-60 | -1.69E-46 |
| MS.gene050762 | MS.gene56431 | 0.805577 | 1.25E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene56431 | 0.807266 | 5.50E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene56431 | 0.817967 | 2.51E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene56431 | 0.825524 | 4.47E-54 | -1.69E-46 |
| MS.gene050820 | MS.gene56431 | 0.821169 | 4.66E-53 | -1.69E-46 |
| MS.gene050826 | MS.gene56431 | 0.856224 | 3.72E-62 | -1.69E-46 |
| MS.gene050844 | MS.gene56431 | 0.823994 | 1.03E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene56431 | 0.855434 | 6.32E-62 | -1.69E-46 |
| MS.gene051198 | MS.gene56431 | 0.811705 | 6.12E-51 | -1.69E-46 |
| MS.gene051243 | MS.gene56431 | 0.819264 | 1.27E-52 | -1.69E-46 |
| MS.gene051368 | MS.gene56431 | 0.823968 | 1.04E-53 | -1.69E-46 |
| MS.gene051370 | MS.gene56431 | 0.805652 | 1.20E-49 | -1.69E-46 |
| MS.gene051452 | MS.gene56431 | 0.827239 | 1.75E-54 | -1.69E-46 |
| MS.gene051535 | MS.gene56431 | 0.824681 | 7.08E-54 | -1.69E-46 |
| MS.gene051800 | MS.gene56431 | 0.802776 | 4.78E-49 | -1.69E-46 |
| MS.gene051924 | MS.gene56431 | -0.83059 | 2.70E-55 | -1.69E-46 |
| MS.gene051926 | MS.gene56431 | -0.840754 | 7.19E-58 | -1.69E-46 |
| MS.gene051983 | MS.gene56431 | 0.801482 | 8.82E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene56431 | 0.807292 | 5.43E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene56431 | 0.816739 | 4.73E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene56431 | 0.821396 | 4.13E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene56431 | 0.807845 | 4.14E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene56431 | 0.859734 | 3.37E-63 | -1.69E-46 |
| MS.gene052519 | MS.gene56431 | 0.802627 | 5.13E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene56431 | 0.812004 | 5.27E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene56431 | 0.830042 | 3.67E-55 | -1.69E-46 |
| MS.gene052675 | MS.gene56431 | 0.828536 | 8.51E-55 | -1.69E-46 |
| MS.gene052677 | MS.gene56431 | 0.834954 | 2.22E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene56431 | 0.845812 | 3.22E-59 | -1.69E-46 |
| MS.gene05288 | MS.gene56431 | 0.819989 | 8.69E-53 | -1.69E-46 |
| MS.gene052896 | MS.gene56431 | 0.812607 | 3.89E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene56431 | 0.818258 | 2.15E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene56431 | 0.858873 | 6.11E-63 | -1.69E-46 |
| MS.gene053166 | MS.gene56431 | 0.864482 | 1.18E-64 | -1.69E-46 |
| MS.gene053201 | MS.gene56431 | 0.823665 | 1.23E-53 | -1.69E-46 |
| MS.gene053216 | MS.gene56431 | 0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene053494 | MS.gene56431 | 0.863928 | 1.75E-64 | -1.69E-46 |
| MS.gene05350 | MS.gene56431 | 0.813171 | 2.93E-51 | -1.69E-46 |
| MS.gene053565 | MS.gene56431 | 0.802496 | 5.46E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene56431 | 0.849342 | 3.44E-60 | -1.69E-46 |
| MS.gene05415 | MS.gene56431 | 0.829673 | 4.51E-55 | -1.69E-46 |
| MS.gene05417 | MS.gene56431 | 0.810566 | 1.08E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene56431 | 0.825967 | 3.51E-54 | -1.69E-46 |
| MS.gene054379 | MS.gene56431 | 0.835305 | 1.81E-56 | -1.69E-46 |
| MS.gene054405 | MS.gene56431 | 0.84898 | 4.34E-60 | -1.69E-46 |
| MS.gene054467 | MS.gene56431 | 0.802652 | 5.07E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene56431 | 0.824194 | 9.21E-54 | -1.69E-46 |
| MS.gene054472 | MS.gene56431 | 0.826478 | 2.65E-54 | -1.69E-46 |
| MS.gene054542 | MS.gene56431 | 0.80592 | 1.06E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene56431 | 0.839584 | 1.45E-57 | -1.69E-46 |
| MS.gene054657 | MS.gene56431 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene054930 | MS.gene56431 | 0.841716 | 4.01E-58 | -1.69E-46 |
| MS.gene05510 | MS.gene56431 | 0.885875 | 5.57E-72 | -1.69E-46 |
| MS.gene055227 | MS.gene56431 | 0.828499 | 8.69E-55 | -1.69E-46 |
| MS.gene055230 | MS.gene56431 | 0.811144 | 8.11E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene56431 | 0.8333 | 5.77E-56 | -1.69E-46 |
| MS.gene055358 | MS.gene56431 | 0.829789 | 4.23E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene56431 | 0.823805 | 1.14E-53 | -1.69E-46 |
| MS.gene055608 | MS.gene56431 | 0.813587 | 2.37E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene56431 | 0.827397 | 1.60E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene56431 | 0.897432 | 1.41E-76 | -1.69E-46 |
| MS.gene055818 | MS.gene56431 | 0.857998 | 1.11E-62 | -1.69E-46 |
| MS.gene055926 | MS.gene56431 | 0.845569 | 3.74E-59 | -1.69E-46 |
| MS.gene05604 | MS.gene56431 | 0.829272 | 5.65E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene56431 | 0.843419 | 1.42E-58 | -1.69E-46 |
| MS.gene056506 | MS.gene56431 | 0.855693 | 5.32E-62 | -1.69E-46 |
| MS.gene056873 | MS.gene56431 | 0.80128 | 9.70E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene56431 | 0.848874 | 4.64E-60 | -1.69E-46 |
| MS.gene057060 | MS.gene56431 | 0.811473 | 6.88E-51 | -1.69E-46 |
| MS.gene057156 | MS.gene56431 | -0.818619 | 1.78E-52 | -1.69E-46 |
| MS.gene057194 | MS.gene56431 | 0.8242 | 9.18E-54 | -1.69E-46 |
| MS.gene057394 | MS.gene56431 | 0.848563 | 5.66E-60 | -1.69E-46 |
| MS.gene057395 | MS.gene56431 | 0.837461 | 5.13E-57 | -1.69E-46 |
| MS.gene057444 | MS.gene56431 | 0.807433 | 5.07E-50 | -1.69E-46 |
| MS.gene05761 | MS.gene56431 | 0.820814 | 5.62E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene56431 | 0.812204 | 4.77E-51 | -1.69E-46 |
| MS.gene057750 | MS.gene56431 | 0.821752 | 3.42E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene56431 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene56431 | 0.808076 | 3.70E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene56431 | 0.813588 | 2.37E-51 | -1.69E-46 |
| MS.gene058139 | MS.gene56431 | 0.825882 | 3.68E-54 | -1.69E-46 |
| MS.gene058146 | MS.gene56431 | 0.841783 | 3.85E-58 | -1.69E-46 |
| MS.gene058399 | MS.gene56431 | 0.803817 | 2.91E-49 | -1.69E-46 |
| MS.gene058435 | MS.gene56431 | 0.824905 | 6.27E-54 | -1.69E-46 |
| MS.gene058548 | MS.gene56431 | 0.803719 | 3.05E-49 | -1.69E-46 |
| MS.gene058595 | MS.gene56431 | 0.850577 | 1.55E-60 | -1.69E-46 |
| MS.gene058979 | MS.gene56431 | 0.830026 | 3.70E-55 | -1.69E-46 |
| MS.gene059128 | MS.gene56431 | 0.865794 | 4.56E-65 | -1.69E-46 |
| MS.gene059161 | MS.gene56431 | 0.870638 | 1.25E-66 | -1.69E-46 |
| MS.gene059283 | MS.gene56431 | 0.805368 | 1.38E-49 | -1.69E-46 |
| MS.gene059382 | MS.gene56431 | 0.807932 | 3.97E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene56431 | 0.829876 | 4.03E-55 | -1.69E-46 |
| MS.gene05973 | MS.gene56431 | 0.804083 | 2.56E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene56431 | 0.825827 | 3.79E-54 | -1.69E-46 |
| MS.gene05979 | MS.gene56431 | 0.801002 | 1.11E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene56431 | 0.814499 | 1.49E-51 | -1.69E-46 |
| MS.gene059952 | MS.gene56431 | 0.804583 | 2.01E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene56431 | 0.836503 | 9.01E-57 | -1.69E-46 |
| MS.gene060137 | MS.gene56431 | -0.811848 | 5.70E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene56431 | 0.843545 | 1.31E-58 | -1.69E-46 |
| MS.gene060322 | MS.gene56431 | 0.89886 | 3.51E-77 | -1.69E-46 |
| MS.gene06052 | MS.gene56431 | 0.823882 | 1.09E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene56431 | 0.803219 | 3.87E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene56431 | 0.875037 | 4.22E-68 | -1.69E-46 |
| MS.gene060685 | MS.gene56431 | 0.877366 | 6.66E-69 | -1.69E-46 |
| MS.gene060802 | MS.gene56431 | 0.85486 | 9.29E-62 | -1.69E-46 |
| MS.gene060803 | MS.gene56431 | 0.838037 | 3.65E-57 | -1.69E-46 |
| MS.gene060918 | MS.gene56431 | 0.800616 | 1.33E-48 | -1.69E-46 |
| MS.gene060942 | MS.gene56431 | 0.81693 | 4.29E-52 | -1.69E-46 |
| MS.gene060943 | MS.gene56431 | 0.802367 | 5.80E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene56431 | 0.8644 | 1.25E-64 | -1.69E-46 |
| MS.gene061002 | MS.gene56431 | 0.819269 | 1.27E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene56431 | -0.802225 | 6.21E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene56431 | -0.821674 | 3.56E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56431.t1 | MTR_4g083970 | 98.137 | 161 | 3 | 0 | 1 | 161 | 130 | 290 | 4.45e-117 | 333 |
| MS.gene56431.t1 | MTR_1g079260 | 30.688 | 189 | 101 | 6 | 1 | 162 | 88 | 273 | 3.00e-26 | 100 |
| MS.gene56431.t1 | MTR_1g079380 | 30.508 | 177 | 102 | 2 | 4 | 161 | 91 | 265 | 7.90e-26 | 100 |
| MS.gene56431.t1 | MTR_1g079370 | 29.444 | 180 | 106 | 3 | 1 | 161 | 88 | 265 | 4.25e-24 | 94.7 |
| MS.gene56431.t1 | MTR_1g079460 | 29.050 | 179 | 108 | 2 | 1 | 162 | 91 | 267 | 2.20e-22 | 90.5 |
| MS.gene56431.t1 | MTR_1g079180 | 30.000 | 180 | 102 | 5 | 1 | 161 | 91 | 265 | 3.26e-21 | 87.0 |
| MS.gene56431.t1 | MTR_1g079200 | 28.729 | 181 | 100 | 4 | 4 | 161 | 90 | 264 | 1.03e-19 | 83.2 |
| MS.gene56431.t1 | MTR_1g079140 | 27.885 | 208 | 101 | 4 | 1 | 161 | 101 | 306 | 1.83e-19 | 83.2 |
| MS.gene56431.t1 | MTR_1g078470 | 28.161 | 174 | 103 | 4 | 5 | 161 | 16 | 184 | 3.26e-19 | 80.1 |
| MS.gene56431.t1 | MTR_7g112560 | 29.310 | 174 | 107 | 4 | 2 | 161 | 86 | 257 | 6.49e-19 | 80.9 |
| MS.gene56431.t1 | MTR_7g112550 | 31.980 | 197 | 92 | 9 | 2 | 162 | 94 | 284 | 1.28e-18 | 80.5 |
| MS.gene56431.t1 | MTR_1g079850 | 28.272 | 191 | 102 | 5 | 4 | 162 | 95 | 282 | 3.18e-18 | 79.7 |
| MS.gene56431.t1 | MTR_1g079130 | 28.272 | 191 | 92 | 5 | 14 | 161 | 1 | 189 | 6.06e-17 | 74.3 |
| MS.gene56431.t1 | MTR_1g083580 | 29.630 | 189 | 97 | 6 | 4 | 161 | 98 | 281 | 1.18e-16 | 75.1 |
| MS.gene56431.t1 | MTR_4g023570 | 27.329 | 161 | 107 | 3 | 2 | 161 | 86 | 237 | 2.32e-16 | 73.9 |
| MS.gene56431.t1 | MTR_1g079410 | 27.624 | 181 | 108 | 4 | 1 | 161 | 96 | 273 | 3.60e-16 | 73.9 |
| MS.gene56431.t1 | MTR_1g100597 | 27.128 | 188 | 107 | 4 | 1 | 161 | 88 | 272 | 3.79e-16 | 73.9 |
| MS.gene56431.t1 | MTR_1g079230 | 26.596 | 188 | 109 | 3 | 1 | 161 | 88 | 273 | 1.37e-15 | 72.4 |
| MS.gene56431.t1 | MTR_1g100613 | 27.586 | 174 | 94 | 3 | 1 | 144 | 73 | 244 | 2.05e-15 | 71.2 |
| MS.gene56431.t1 | MTR_1g026510 | 27.835 | 194 | 102 | 8 | 4 | 162 | 94 | 284 | 1.53e-13 | 67.0 |
| MS.gene56431.t1 | MTR_7g103380 | 25.806 | 186 | 110 | 6 | 1 | 162 | 104 | 285 | 7.78e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56431.t1 | AT4G19840 | 53.333 | 165 | 72 | 3 | 1 | 162 | 84 | 246 | 2.43e-59 | 184 |
| MS.gene56431.t1 | AT4G19850 | 49.383 | 162 | 77 | 3 | 5 | 161 | 15 | 176 | 5.22e-51 | 161 |
| MS.gene56431.t1 | AT4G19850 | 49.383 | 162 | 77 | 3 | 5 | 161 | 57 | 218 | 1.23e-50 | 162 |
| MS.gene56431.t1 | AT1G33920 | 44.079 | 152 | 81 | 3 | 14 | 161 | 13 | 164 | 1.80e-43 | 142 |
| MS.gene56431.t1 | AT4G19850 | 49.275 | 138 | 65 | 3 | 5 | 137 | 57 | 194 | 1.49e-39 | 133 |
| MS.gene56431.t1 | AT1G65390 | 40.361 | 166 | 93 | 4 | 1 | 161 | 246 | 410 | 4.48e-39 | 137 |
| MS.gene56431.t1 | AT5G45090 | 34.756 | 164 | 101 | 5 | 3 | 160 | 145 | 308 | 2.14e-27 | 104 |
| MS.gene56431.t1 | AT5G45090 | 34.756 | 164 | 101 | 5 | 3 | 160 | 167 | 330 | 2.42e-27 | 104 |
| MS.gene56431.t1 | AT2G02240 | 33.708 | 178 | 97 | 4 | 1 | 160 | 142 | 316 | 2.82e-24 | 96.3 |
| MS.gene56431.t1 | AT5G45080 | 35.294 | 153 | 93 | 4 | 14 | 160 | 238 | 390 | 7.26e-23 | 93.6 |
| MS.gene56431.t1 | AT1G80110 | 33.523 | 176 | 99 | 3 | 1 | 160 | 82 | 255 | 9.38e-23 | 91.3 |
| MS.gene56431.t1 | AT2G26820 | 31.818 | 154 | 98 | 3 | 14 | 160 | 308 | 461 | 4.44e-21 | 89.0 |
| MS.gene56431.t1 | AT2G02360 | 34.270 | 178 | 91 | 7 | 3 | 160 | 97 | 268 | 4.80e-21 | 87.0 |
| MS.gene56431.t1 | AT2G26820 | 31.818 | 154 | 98 | 3 | 14 | 160 | 324 | 477 | 5.95e-21 | 88.6 |
| MS.gene56431.t1 | AT2G02250 | 30.726 | 179 | 100 | 7 | 2 | 160 | 129 | 303 | 1.08e-20 | 86.7 |
| MS.gene56431.t1 | AT5G45070 | 33.133 | 166 | 103 | 6 | 4 | 162 | 190 | 354 | 8.41e-20 | 84.7 |
| MS.gene56431.t1 | AT2G02340 | 27.957 | 186 | 101 | 6 | 3 | 160 | 121 | 301 | 1.57e-16 | 75.1 |
| MS.gene56431.t1 | AT5G24560 | 27.778 | 162 | 65 | 4 | 1 | 162 | 84 | 193 | 1.91e-14 | 68.2 |
| MS.gene56431.t1 | AT2G02230 | 43.590 | 78 | 42 | 1 | 1 | 78 | 113 | 188 | 2.37e-14 | 69.3 |
| MS.gene56431.t1 | AT2G02320 | 28.889 | 180 | 102 | 6 | 3 | 160 | 123 | 298 | 9.36e-14 | 67.8 |
| MS.gene56431.t1 | AT2G02310 | 27.568 | 185 | 106 | 5 | 1 | 162 | 126 | 305 | 3.52e-12 | 63.2 |
| MS.gene56431.t1 | AT3G53000 | 28.571 | 182 | 102 | 7 | 3 | 160 | 107 | 284 | 4.98e-12 | 62.8 |
Find 42 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATCCATTTCCAATAATT+TGG | 0.178400 | 4.4:+28906140 | None:intergenic |
| AACGTTGTTTGTAGAAATAT+TGG | 0.268294 | 4.4:-28906235 | None:intergenic |
| GGGCAGAAGATCCAAATTAT+TGG | 0.313201 | 4.4:-28906151 | MS.gene56431:CDS |
| GCAACACAAGGAAAATTTAA+TGG | 0.313866 | 4.4:-28905506 | MS.gene56431:CDS |
| AATGTTCGACTTGTCCTTCC+AGG | 0.347328 | 4.4:-28905541 | MS.gene56431:CDS |
| AAGATCCAAATTATTGGAAA+TGG | 0.355234 | 4.4:-28906145 | MS.gene56431:CDS |
| GTGTGTTGGTTAGAAGTTCA+TGG | 0.382382 | 4.4:-28905661 | MS.gene56431:CDS |
| GAAACTTGATATAAAATTCC+TGG | 0.384782 | 4.4:+28905613 | None:intergenic |
| TGTTGCTGCTTCTTTCCTCC+TGG | 0.398539 | 4.4:+28905523 | None:intergenic |
| AGCTCAGGGTTGGGAACTTC+CGG | 0.410700 | 4.4:-28905566 | MS.gene56431:CDS |
| CAACATGAAACAGTTGGCTT+TGG | 0.410721 | 4.4:+28906199 | None:intergenic |
| GATGCTACAAACTCACCAAC+TGG | 0.464067 | 4.4:+28905454 | None:intergenic |
| ACGTTGTTTGTAGAAATATT+GGG | 0.469595 | 4.4:-28906234 | None:intergenic |
| AAAAGATCCAGCTCAGGGTT+GGG | 0.476440 | 4.4:-28905575 | MS.gene56431:CDS |
| TGCATACAACATGAAACAGT+TGG | 0.479048 | 4.4:+28906193 | None:intergenic |
| GCTGCTTCTTTCCTCCTGGA+AGG | 0.481544 | 4.4:+28905527 | None:intergenic |
| AATGTTAAAAGATCCAGCTC+AGG | 0.485668 | 4.4:-28905581 | MS.gene56431:CDS |
| CAAAAGCACTCTCAATCACC+TGG | 0.496488 | 4.4:-28906172 | MS.gene56431:CDS |
| GGAAGTTCCCAACCCTGAGC+TGG | 0.506652 | 4.4:+28905568 | None:intergenic |
| GTTAAGAGCACGGTGGATAG+AGG | 0.513931 | 4.4:-28905479 | MS.gene56431:CDS |
| TAAAAGATCCAGCTCAGGGT+TGG | 0.544106 | 4.4:-28905576 | MS.gene56431:CDS |
| ATATCAATGTTTGAATATGA+AGG | 0.554106 | 4.4:-28905406 | MS.gene56431:CDS |
| AGGACAAGTCGAACATTCAC+CGG | 0.560100 | 4.4:+28905547 | None:intergenic |
| TTGAATATGAAGGTGGTATG+TGG | 0.566199 | 4.4:-28905396 | MS.gene56431:CDS |
| ATAATTTGGATCTTCTGCCC+AGG | 0.570223 | 4.4:+28906154 | None:intergenic |
| AGGAAAGAAGCAGCAACACA+AGG | 0.570643 | 4.4:-28905518 | MS.gene56431:CDS |
| AAACAAGGGCTTGTCATCAA+AGG | 0.587551 | 4.4:-28905373 | MS.gene56431:CDS |
| GAAGGTGGTATGTGGAAACA+AGG | 0.592943 | 4.4:-28905388 | MS.gene56431:CDS |
| GACACAAGGAAGCTTTCACC+AGG | 0.601273 | 4.4:-28905631 | MS.gene56431:CDS |
| AGGCATTGCTATCAAACCAA+AGG | 0.606104 | 4.4:-28905353 | MS.gene56431:CDS |
| TAATGGAAAAGTTAAGAGCA+CGG | 0.611140 | 4.4:-28905489 | MS.gene56431:CDS |
| ATGTTAAAAGATCCAGCTCA+GGG | 0.613664 | 4.4:-28905580 | MS.gene56431:CDS |
| TCAATGTTTGAATATGAAGG+TGG | 0.618732 | 4.4:-28905403 | MS.gene56431:CDS |
| TTCATGGAAAATTCGACACA+AGG | 0.625618 | 4.4:-28905645 | MS.gene56431:CDS |
| AAGGTGGTATGTGGAAACAA+GGG | 0.657478 | 4.4:-28905387 | MS.gene56431:CDS |
| CAGTGAGGGGATGACTGAGG+TGG | 0.662615 | 4.4:-28905698 | MS.gene56431:CDS |
| CTGAACTGAAGAGAGTGTGT+TGG | 0.663119 | 4.4:-28905675 | MS.gene56431:CDS |
| TTGCAGTGAGGGGATGACTG+AGG | 0.666138 | 4.4:-28905701 | MS.gene56431:intron |
| CGGTGGATAGAGGTTCCAGT+TGG | 0.671489 | 4.4:-28905469 | MS.gene56431:CDS |
| AAAAGCACTCTCAATCACCT+GGG | 0.675178 | 4.4:-28906171 | MS.gene56431:CDS |
| GTTCGACTTGTCCTTCCAGG+AGG | 0.692090 | 4.4:-28905538 | MS.gene56431:CDS |
| TGGAAAAGTTAAGAGCACGG+TGG | 0.775430 | 4.4:-28905486 | MS.gene56431:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAAATTGTTGATATAAT+AGG | + | chr4.4:28905497-28905516 | None:intergenic | 15.0% |
| !!! | TTTTTCTAAGTTATATGTAA+AGG | + | chr4.4:28905602-28905621 | None:intergenic | 15.0% |
| !! | ATATCAATGTTTGAATATGA+AGG | - | chr4.4:28906163-28906182 | MS.gene56431:CDS | 20.0% |
| !! | TAGGAAAAGATAATGATATA+AGG | + | chr4.4:28905478-28905497 | None:intergenic | 20.0% |
| !! | TGAAATTATTTGTAATCACT+GGG | + | chr4.4:28905519-28905538 | None:intergenic | 20.0% |
| !! | TTGAAATTATTTGTAATCAC+TGG | + | chr4.4:28905520-28905539 | None:intergenic | 20.0% |
| ! | AAACATAGCTTGATCTAATT+AGG | + | chr4.4:28905849-28905868 | None:intergenic | 25.0% |
| ! | AAGATCCAAATTATTGGAAA+TGG | - | chr4.4:28905424-28905443 | MS.gene56431:CDS | 25.0% |
| ! | ATTACAAGCTAATTCTATCT+AGG | - | chr4.4:28905757-28905776 | MS.gene56431:intron | 25.0% |
| ! | GAAACTTGATATAAAATTCC+TGG | + | chr4.4:28905959-28905978 | None:intergenic | 25.0% |
| ! | TGTATCCATTTCCAATAATT+TGG | + | chr4.4:28905432-28905451 | None:intergenic | 25.0% |
| GCAACACAAGGAAAATTTAA+TGG | - | chr4.4:28906063-28906082 | MS.gene56431:intron | 30.0% | |
| TAATGGAAAAGTTAAGAGCA+CGG | - | chr4.4:28906080-28906099 | MS.gene56431:intron | 30.0% | |
| TCAATGTTTGAATATGAAGG+TGG | - | chr4.4:28906166-28906185 | MS.gene56431:CDS | 30.0% | |
| TTTAGGGACTAAATTGAAGA+TGG | + | chr4.4:28905663-28905682 | None:intergenic | 30.0% | |
| TTTGTAGCATCTGAAAAAGA+TGG | - | chr4.4:28906127-28906146 | MS.gene56431:CDS | 30.0% | |
| AATGTTAAAAGATCCAGCTC+AGG | - | chr4.4:28905988-28906007 | MS.gene56431:intron | 35.0% | |
| ATGTTAAAAGATCCAGCTCA+GGG | - | chr4.4:28905989-28906008 | MS.gene56431:intron | 35.0% | |
| GGATACAGCAAAAAGATGTT+AGG | - | chr4.4:28905445-28905464 | MS.gene56431:CDS | 35.0% | |
| TGCATACAACATGAAACAGT+TGG | + | chr4.4:28905379-28905398 | None:intergenic | 35.0% | |
| TTCATGGAAAATTCGACACA+AGG | - | chr4.4:28905924-28905943 | MS.gene56431:intron | 35.0% | |
| TTGAATATGAAGGTGGTATG+TGG | - | chr4.4:28906173-28906192 | MS.gene56431:CDS | 35.0% | |
| !! | TTCTATCTAGGACTTTTGTC+TGG | - | chr4.4:28905769-28905788 | MS.gene56431:intron | 35.0% |
| AAACAAGGGCTTGTCATCAA+AGG | - | chr4.4:28906196-28906215 | MS.gene56431:CDS | 40.0% | |
| AAGGTGGTATGTGGAAACAA+GGG | - | chr4.4:28906182-28906201 | MS.gene56431:CDS | 40.0% | |
| ATAATTTGGATCTTCTGCCC+AGG | + | chr4.4:28905418-28905437 | None:intergenic | 40.0% | |
| GGGCAGAAGATCCAAATTAT+TGG | - | chr4.4:28905418-28905437 | MS.gene56431:CDS | 40.0% | |
| GTAGCATCTGAAAAAGATGG+CGG | - | chr4.4:28906130-28906149 | MS.gene56431:CDS | 40.0% | |
| ! | AAGCTATGTTTTGCAGTGAG+GGG | - | chr4.4:28905858-28905877 | MS.gene56431:intron | 40.0% |
| ! | AGGCATTGCTATCAAACCAA+AGG | - | chr4.4:28906216-28906235 | MS.gene56431:CDS | 40.0% |
| ! | CAACATGAAACAGTTGGCTT+TGG | + | chr4.4:28905373-28905392 | None:intergenic | 40.0% |
| ! | CAAGCTATGTTTTGCAGTGA+GGG | - | chr4.4:28905857-28905876 | MS.gene56431:intron | 40.0% |
| ! | TCAAGCTATGTTTTGCAGTG+AGG | - | chr4.4:28905856-28905875 | MS.gene56431:intron | 40.0% |
| !! | AAAAGCACTCTCAATCACCT+GGG | - | chr4.4:28905398-28905417 | MS.gene56431:CDS | 40.0% |
| !! | GTGTGTTGGTTAGAAGTTCA+TGG | - | chr4.4:28905908-28905927 | MS.gene56431:intron | 40.0% |
| AAAAGATCCAGCTCAGGGTT+GGG | - | chr4.4:28905994-28906013 | MS.gene56431:intron | 45.0% | |
| AGGAAAGAAGCAGCAACACA+AGG | - | chr4.4:28906051-28906070 | MS.gene56431:intron | 45.0% | |
| AGGACAAGTCGAACATTCAC+CGG | + | chr4.4:28906025-28906044 | None:intergenic | 45.0% | |
| GAAGGTGGTATGTGGAAACA+AGG | - | chr4.4:28906181-28906200 | MS.gene56431:CDS | 45.0% | |
| GATGCTACAAACTCACCAAC+TGG | + | chr4.4:28906118-28906137 | None:intergenic | 45.0% | |
| TAAAAGATCCAGCTCAGGGT+TGG | - | chr4.4:28905993-28906012 | MS.gene56431:intron | 45.0% | |
| ! | AATGTTCGACTTGTCCTTCC+AGG | - | chr4.4:28906028-28906047 | MS.gene56431:intron | 45.0% |
| ! | CTGAACTGAAGAGAGTGTGT+TGG | - | chr4.4:28905894-28905913 | MS.gene56431:intron | 45.0% |
| ! | TGAAAAAGATGGCGGTGAGA+TGG | - | chr4.4:28906138-28906157 | MS.gene56431:CDS | 45.0% |
| ! | TGGAAAAGTTAAGAGCACGG+TGG | - | chr4.4:28906083-28906102 | MS.gene56431:intron | 45.0% |
| !! | CAAAAGCACTCTCAATCACC+TGG | - | chr4.4:28905397-28905416 | MS.gene56431:CDS | 45.0% |
| !! | TAAAAAATTTATATAGTTTA+GGG | + | chr4.4:28905679-28905698 | None:intergenic | 5.0% |
| !! | TTAAAAAATTTATATAGTTT+AGG | + | chr4.4:28905680-28905699 | None:intergenic | 5.0% |
| GACACAAGGAAGCTTTCACC+AGG | - | chr4.4:28905938-28905957 | MS.gene56431:intron | 50.0% | |
| ! | GTTAAGAGCACGGTGGATAG+AGG | - | chr4.4:28906090-28906109 | MS.gene56431:intron | 50.0% |
| ! | TGTTGCTGCTTCTTTCCTCC+TGG | + | chr4.4:28906049-28906068 | None:intergenic | 50.0% |
| AGCTCAGGGTTGGGAACTTC+CGG | - | chr4.4:28906003-28906022 | MS.gene56431:intron | 55.0% | |
| CGGTGGATAGAGGTTCCAGT+TGG | - | chr4.4:28906100-28906119 | MS.gene56431:intron | 55.0% | |
| GCTGCTTCTTTCCTCCTGGA+AGG | + | chr4.4:28906045-28906064 | None:intergenic | 55.0% | |
| TTGCAGTGAGGGGATGACTG+AGG | - | chr4.4:28905868-28905887 | MS.gene56431:intron | 55.0% | |
| ! | GTTCGACTTGTCCTTCCAGG+AGG | - | chr4.4:28906031-28906050 | MS.gene56431:intron | 55.0% |
| CAGTGAGGGGATGACTGAGG+TGG | - | chr4.4:28905871-28905890 | MS.gene56431:intron | 60.0% | |
| GGAAGTTCCCAACCCTGAGC+TGG | + | chr4.4:28906004-28906023 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 28905348 | 28906243 | 28905348 | ID=MS.gene56431 |
| chr4.4 | mRNA | 28905348 | 28906243 | 28905348 | ID=MS.gene56431.t1;Parent=MS.gene56431 |
| chr4.4 | exon | 28906125 | 28906243 | 28906125 | ID=MS.gene56431.t1.exon1;Parent=MS.gene56431.t1 |
| chr4.4 | CDS | 28906125 | 28906243 | 28906125 | ID=cds.MS.gene56431.t1;Parent=MS.gene56431.t1 |
| chr4.4 | exon | 28905348 | 28905717 | 28905348 | ID=MS.gene56431.t1.exon2;Parent=MS.gene56431.t1 |
| chr4.4 | CDS | 28905348 | 28905717 | 28905348 | ID=cds.MS.gene56431.t1;Parent=MS.gene56431.t1 |
| Gene Sequence |
| Protein sequence |
