AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052832

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052832.t1 MTR_1g035570 79.279 333 50 5 33 360 87 405 0.0 508
MS.gene052832.t1 MTR_1g035490 66.276 341 51 6 50 351 15 330 5.36e-138 396
MS.gene052832.t1 MTR_1g035620 41.193 352 137 13 33 348 91 408 3.03e-66 215
MS.gene052832.t1 MTR_1g035640 32.927 410 152 13 33 350 87 465 1.47e-48 170
MS.gene052832.t1 MTR_1g050845 35.647 317 128 10 33 316 91 364 1.24e-42 159
MS.gene052832.t1 MTR_6g061070 30.809 383 188 11 32 348 98 469 3.87e-40 148
MS.gene052832.t1 MTR_1g035520 43.011 93 49 3 261 349 291 383 1.48e-15 77.8
MS.gene052832.t1 MTR_5g044670 30.168 179 106 6 87 248 132 308 5.81e-14 72.4
MS.gene052832.t1 MTR_7g050590 26.991 226 131 8 35 243 72 280 4.11e-11 63.5
MS.gene052832.t1 MTR_1g108780 25.221 226 140 7 33 244 81 291 7.02e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTGGGCTTGGTTTCT+TGG 0.120870 1.4:+20537352 None:intergenic
AATACAGATGTTTGCAATAA+TGG 0.182705 1.4:+20536326 None:intergenic
GAAACACCTAGTAATTTCTT+TGG 0.254190 1.4:+20537338 None:intergenic
AAATATTGTGGAGTTGAAAA+TGG 0.256652 1.4:-20536551 MS.gene052832:CDS
GCATAAGACATTCAAGGTTT+TGG 0.285417 1.4:-20536721 MS.gene052832:CDS
GGAGTGTGGAAGTGGGTTTA+AGG 0.287521 1.4:-20537517 MS.gene052832:CDS
TGATAGTGTTTCTCTATTGA+TGG 0.293406 1.4:-20539379 MS.gene052832:intron
TGTTTGCAATAATGGATTCT+TGG 0.336287 1.4:+20536334 None:intergenic
CTTGGAGAACAAACAGCTAA+TGG 0.342183 1.4:+20536352 None:intergenic
GTGTTTGATGTTTCAATTGC+AGG 0.344942 1.4:-20536382 MS.gene052832:CDS
ATAGTAACAATGAATAAAAT+TGG 0.350107 1.4:+20537383 None:intergenic
AAACACCTAGTAATTTCTTT+GGG 0.357748 1.4:+20537339 None:intergenic
AATTATTATGAGAGTATTCA+TGG 0.374261 1.4:-20536502 MS.gene052832:CDS
TCCGTTCATCAATCAACTTA+AGG 0.375210 1.4:+20536410 None:intergenic
CCTAGTAATTTCTTTGGGCT+TGG 0.381459 1.4:+20537344 None:intergenic
TACCGAATTGCCGCTGAGCT+TGG 0.400116 1.4:+20539489 None:intergenic
AACAGCTTCATTAACAGCTT+CGG 0.400694 1.4:+20537750 None:intergenic
ATAATTTATTTCTAAAGTAT+TGG 0.414924 1.4:+20538478 None:intergenic
TCATTAACAGCTTCGGATGT+TGG 0.416976 1.4:+20537757 None:intergenic
GAGGTGGTTGGAAAATATTC+AGG 0.424095 1.4:-20537463 MS.gene052832:CDS
AAAGAATGCAAAGTGGAGAT+TGG 0.426567 1.4:-20536445 MS.gene052832:CDS
TACCAAGGAAAACTCTTGTA+TGG 0.429636 1.4:-20537486 MS.gene052832:CDS
CCAAGCCCAAAGAAATTACT+AGG 0.436880 1.4:-20537344 MS.gene052832:intron
GTCTTATGCTTCATCACATC+AGG 0.448698 1.4:+20536735 None:intergenic
GAGCTTGGCTGAACTGCAAT+CGG 0.449915 1.4:+20539504 None:intergenic
CAAGCTCAAAGGACCAGAAA+TGG 0.451340 1.4:-20538275 MS.gene052832:CDS
AATATTGTGGAGTTGAAAAT+GGG 0.451536 1.4:-20536550 MS.gene052832:CDS
GATGAAGCATAAGACATTCA+AGG 0.464182 1.4:-20536727 MS.gene052832:CDS
CAAAGTGGCCATGGTGAAGA+AGG 0.465563 1.4:-20537791 MS.gene052832:CDS
TGTCATTATAACTTGAAAAG+AGG 0.472819 1.4:+20537717 None:intergenic
GTCATTATAACTTGAAAAGA+GGG 0.482400 1.4:+20537718 None:intergenic
GGTGCTGAAGCCACTGAAGA+AGG 0.486856 1.4:-20538431 MS.gene052832:CDS
AGCCAAGCTCAGCGGCAATT+CGG 0.488549 1.4:-20539491 MS.gene052832:intron
CCAAAGGAGTTTATGAAAAG+AGG 0.490308 1.4:-20536583 MS.gene052832:CDS
TCATGGCTGCAGATTCAGTA+AGG 0.502175 1.4:-20536485 MS.gene052832:CDS
TGAAAATGGGGAGGAAGTCA+TGG 0.505391 1.4:-20536537 MS.gene052832:CDS
ACTTTCTTCACCTTCTTCTG+TGG 0.510570 1.4:+20538343 None:intergenic
GATGATGAAGCCACAGAAGA+AGG 0.515501 1.4:-20538353 MS.gene052832:CDS
ACTTTCTTGAACTTCTTCTG+TGG 0.518992 1.4:+20538388 None:intergenic
GAGTTCTGATGAAGTTGAAA+TGG 0.525927 1.4:-20538303 MS.gene052832:CDS
AGGATGTCATGAAAATATTG+TGG 0.531604 1.4:-20536563 MS.gene052832:CDS
ATGACACAATCTTACGTCAA+TGG 0.537902 1.4:-20537700 MS.gene052832:CDS
AATATCAAAGCAGTTGATGA+AGG 0.544297 1.4:-20538452 MS.gene052832:CDS
ACTTGACTCGCCTTCTTCAG+TGG 0.548239 1.4:+20538421 None:intergenic
TAATGAAAGGAGTGTGGAAG+TGG 0.550991 1.4:-20537525 MS.gene052832:CDS
CAATAAGAGTTGGTAATGAA+AGG 0.551596 1.4:-20537538 MS.gene052832:CDS
TGTTCTCTTGAAAACTCACT+TGG 0.555107 1.4:+20537587 None:intergenic
ACCAAGGAAAACTCTTGTAT+GGG 0.558782 1.4:-20537485 MS.gene052832:CDS
GGGACTGCAACAATAAGAGT+TGG 0.570294 1.4:-20537548 MS.gene052832:CDS
TTTGGTGAAGAACACAGCCC+CGG 0.573521 1.4:-20536703 MS.gene052832:intron
AACGACACTGCCATGTCTGT+TGG 0.574077 1.4:+20539530 None:intergenic
TCCTTAAGTTGATTGATGAA+CGG 0.574318 1.4:-20536411 MS.gene052832:CDS
AGTTGGTAATGAAAGGAGTG+TGG 0.575242 1.4:-20537531 MS.gene052832:CDS
AGGTAGTAACAAAGTGGCCA+TGG 0.577811 1.4:-20537800 MS.gene052832:CDS
GAAAACTCTTGTATGGGAGG+TGG 0.585914 1.4:-20537479 MS.gene052832:CDS
GTATTTGTAGAGTGTACCAA+AGG 0.588430 1.4:-20536599 MS.gene052832:intron
ACTCTTGTATGGGAGGTGGT+TGG 0.598499 1.4:-20537475 MS.gene052832:CDS
TTACGTCAATGGCTATCACT+TGG 0.603062 1.4:-20537689 MS.gene052832:intron
ATTTATGAAAGAATGCAAAG+TGG 0.609427 1.4:-20536452 MS.gene052832:CDS
GCTGCAGATTCAGTAAGGGA+TGG 0.619047 1.4:-20536480 MS.gene052832:CDS
TTTAAGGTATTATGATACCA+AGG 0.620069 1.4:-20537501 MS.gene052832:CDS
AAATGGCAAAGCAAGCTCAA+AGG 0.625671 1.4:-20538286 MS.gene052832:CDS
AAGGAAAACTCTTGTATGGG+AGG 0.629797 1.4:-20537482 MS.gene052832:CDS
CTTAGCAGGTAGTAACAAAG+TGG 0.630003 1.4:-20537806 MS.gene052832:intron
TTGTGGAGTTGAAAATGGGG+AGG 0.632079 1.4:-20536546 MS.gene052832:CDS
CATGGCTGCAGATTCAGTAA+GGG 0.636738 1.4:-20536484 MS.gene052832:CDS
AATGAAAGGAGTGTGGAAGT+GGG 0.647980 1.4:-20537524 MS.gene052832:CDS
TCATCTTCATCCAACAGACA+TGG 0.668320 1.4:-20539540 MS.gene052832:CDS
TGCAGTTCAGCCAAGCTCAG+CGG 0.670925 1.4:-20539499 MS.gene052832:CDS
ATATTGTGGAGTTGAAAATG+GGG 0.692849 1.4:-20536549 MS.gene052832:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATTATTTCAAATTCA+CGG + chr1.4:20538938-20538957 None:intergenic 10.0%
!!! ATAATTTATTTCTAAAGTAT+TGG + chr1.4:20537389-20537408 None:intergenic 10.0%
!!! CAAATACATATAATTTTTTT+GGG - chr1.4:20539053-20539072 MS.gene052832:intron 10.0%
!!! GTATTTTAAATTTAAGATTA+AGG + chr1.4:20538915-20538934 None:intergenic 10.0%
!!! TATAATAATCTATTTTGTTT+AGG + chr1.4:20538028-20538047 None:intergenic 10.0%
!!! TCAAATACATATAATTTTTT+TGG - chr1.4:20539052-20539071 MS.gene052832:intron 10.0%
!!! TTTTATTTTCAGATAATTTT+TGG - chr1.4:20537859-20537878 MS.gene052832:intron 10.0%
!! ATAGTAACAATGAATAAAAT+TGG + chr1.4:20538484-20538503 None:intergenic 15.0%
!! ATTTATGTAATTCTTCAATT+TGG - chr1.4:20536409-20536428 MS.gene052832:CDS 15.0%
!! ATTTGAATAGATTTATAGTT+AGG + chr1.4:20539038-20539057 None:intergenic 15.0%
!! TAAAATTATTTCAAATTCAC+GGG + chr1.4:20538937-20538956 None:intergenic 15.0%
!! TCTATATATATAGTTTATGT+TGG + chr1.4:20536754-20536773 None:intergenic 15.0%
!!! ATATTTGGTTTCAAAAAAAT+TGG - chr1.4:20537275-20537294 MS.gene052832:intron 15.0%
!!! ATGAATAACTTATTAGTTTT+TGG - chr1.4:20538868-20538887 MS.gene052832:intron 15.0%
!!! ATTCTTTTTCTATATATATC+AGG + chr1.4:20536877-20536896 None:intergenic 15.0%
!!! TAAAAATATGTGACTTTTTT+TGG - chr1.4:20537897-20537916 MS.gene052832:intron 15.0%
!!! TATTTGGTTTCAAAAAAATT+GGG - chr1.4:20537276-20537295 MS.gene052832:intron 15.0%
!!! TATTTTAAGGTTAAGATATT+TGG - chr1.4:20537361-20537380 MS.gene052832:CDS 15.0%
!!! TGTTGAACAAAAAATATTTT+GGG - chr1.4:20537237-20537256 MS.gene052832:intron 15.0%
!!! TTTTATATAATTAATCCTAC+CGG + chr1.4:20539183-20539202 None:intergenic 15.0%
!! AAAATTATCCTATGAAAATC+TGG + chr1.4:20537008-20537027 None:intergenic 20.0%
!! ATATTCCTTAAATTTCCAAA+CGG - chr1.4:20537214-20537233 MS.gene052832:intron 20.0%
!! ATGATAAGGTTATAAAAAAG+GGG - chr1.4:20538640-20538659 MS.gene052832:intron 20.0%
!! CATGATAAGGTTATAAAAAA+GGG - chr1.4:20538639-20538658 MS.gene052832:intron 20.0%
!! TAACAGAAAAAATTGAATAC+CGG + chr1.4:20539007-20539026 None:intergenic 20.0%
!! TCAAAAAAAACTGAAAGAAT+AGG - chr1.4:20536562-20536581 MS.gene052832:CDS 20.0%
!! TTGGAAATTTAAGGAATATA+GGG + chr1.4:20537213-20537232 None:intergenic 20.0%
!! TTTATATAATTAATCCTACC+GGG + chr1.4:20539182-20539201 None:intergenic 20.0%
!! TTTGGAAATTTAAGGAATAT+AGG + chr1.4:20537214-20537233 None:intergenic 20.0%
!!! AACACTTGAAAGATTTTAAA+GGG - chr1.4:20538296-20538315 MS.gene052832:CDS 20.0%
!!! AATTATTATGAGAGTATTCA+TGG - chr1.4:20539362-20539381 MS.gene052832:intron 20.0%
!!! ATATTTGCAATTTGTGATTT+TGG - chr1.4:20536801-20536820 MS.gene052832:intron 20.0%
!!! GTGTTGAACAAAAAATATTT+TGG - chr1.4:20537236-20537255 MS.gene052832:intron 20.0%
!!! TACTTTTAAGACTTATTCAA+TGG - chr1.4:20537770-20537789 MS.gene052832:CDS 20.0%
! AAACACCTAGTAATTTCTTT+GGG + chr1.4:20538528-20538547 None:intergenic 25.0%
! AAATATTGTGGAGTTGAAAA+TGG - chr1.4:20539313-20539332 MS.gene052832:intron 25.0%
! AAGAGATATCCATATTTGAA+AGG + chr1.4:20537316-20537335 None:intergenic 25.0%
! AATACAGATGTTTGCAATAA+TGG + chr1.4:20539541-20539560 None:intergenic 25.0%
! AATATTGTGGAGTTGAAAAT+GGG - chr1.4:20539314-20539333 MS.gene052832:intron 25.0%
! AGATATATGTGGAGAAATAT+TGG - chr1.4:20537173-20537192 MS.gene052832:intron 25.0%
! ATTTATGAAAGAATGCAAAG+TGG - chr1.4:20539412-20539431 MS.gene052832:intron 25.0%
! CCATGATAAGGTTATAAAAA+AGG - chr1.4:20538638-20538657 MS.gene052832:intron 25.0%
! CTCTTAACTTTCAAATTCAT+TGG - chr1.4:20537331-20537350 MS.gene052832:intron 25.0%
! GTCATTATAACTTGAAAAGA+GGG + chr1.4:20538149-20538168 None:intergenic 25.0%
! GTTCAATGGATATTATAGTT+AGG - chr1.4:20536584-20536603 MS.gene052832:CDS 25.0%
! TATATCTCTTTAAACTTAGC+AGG - chr1.4:20538044-20538063 MS.gene052832:intron 25.0%
! TATTTCTCCACATATATCTT+AGG + chr1.4:20537172-20537191 None:intergenic 25.0%
! TGTCATTATAACTTGAAAAG+AGG + chr1.4:20538150-20538169 None:intergenic 25.0%
! TTAACTTTCAAATTCATTGG+TGG - chr1.4:20537334-20537353 MS.gene052832:intron 25.0%
! TTAGCTAGAAATTACTCATT+TGG - chr1.4:20537681-20537700 MS.gene052832:intron 25.0%
! TTATATAATTAATCCTACCG+GGG + chr1.4:20539181-20539200 None:intergenic 25.0%
! TTCATAATTTATGCACTTCA+AGG + chr1.4:20537825-20537844 None:intergenic 25.0%
!! GAACACTTGAAAGATTTTAA+AGG - chr1.4:20538295-20538314 MS.gene052832:CDS 25.0%
!! TATTTCTTTTGTCCATGATA+AGG - chr1.4:20538626-20538645 MS.gene052832:intron 25.0%
!! TTTAAGGTATTATGATACCA+AGG - chr1.4:20538363-20538382 MS.gene052832:CDS 25.0%
!!! AATATGTGACTTTTTTTGGT+AGG - chr1.4:20537901-20537920 MS.gene052832:intron 25.0%
!!! ACTGTTTTTGAACTGATTTT+TGG - chr1.4:20536442-20536461 MS.gene052832:CDS 25.0%
!!! CCTTTTTTATAACCTTATCA+TGG + chr1.4:20538641-20538660 None:intergenic 25.0%
!!! CTGTTTTTGAACTGATTTTT+GGG - chr1.4:20536443-20536462 MS.gene052832:CDS 25.0%
!!! TGAACAAAAAATATTTTGGG+TGG - chr1.4:20537240-20537259 MS.gene052832:intron 25.0%
AACTGAAAGAATAGGTTCAA+TGG - chr1.4:20536570-20536589 MS.gene052832:CDS 30.0%
AATTGAATACCGGATAAGTT+AGG + chr1.4:20538997-20539016 None:intergenic 30.0%
ACTACCAAACAAAATTTCTC+AGG - chr1.4:20537640-20537659 MS.gene052832:intron 30.0%
AGGATGTCATGAAAATATTG+TGG - chr1.4:20539301-20539320 MS.gene052832:intron 30.0%
AGTTGTGATGATTCACAAAT+AGG - chr1.4:20536717-20536736 MS.gene052832:CDS 30.0%
ATATTGTGGAGTTGAAAATG+GGG - chr1.4:20539315-20539334 MS.gene052832:intron 30.0%
ATTACTCATTTGGTAGACTA+AGG - chr1.4:20537691-20537710 MS.gene052832:CDS 30.0%
CTACCAAACAAAATTTCTCA+GGG - chr1.4:20537641-20537660 MS.gene052832:intron 30.0%
GAAACACCTAGTAATTTCTT+TGG + chr1.4:20538529-20538548 None:intergenic 30.0%
TATGTGGAGAAATATTGGAA+AGG - chr1.4:20537178-20537197 MS.gene052832:intron 30.0%
TCATAGTAGTGTTGTAAAGA+TGG - chr1.4:20538670-20538689 MS.gene052832:intron 30.0%
TTTGCAAAGCCTTTCAAATA+TGG - chr1.4:20537304-20537323 MS.gene052832:intron 30.0%
! AACATGTTCCAGATTTTCAT+AGG - chr1.4:20536997-20537016 MS.gene052832:intron 30.0%
! AATCCCTGAGAAATTTTGTT+TGG + chr1.4:20537647-20537666 None:intergenic 30.0%
! CAAGAAAATTTCCTTTTGGA+TGG + chr1.4:20538721-20538740 None:intergenic 30.0%
! CCAAAAGGAAATTTTCTTGA+GGG - chr1.4:20538722-20538741 MS.gene052832:intron 30.0%
! TAATCCATGATTTGAAACAG+TGG - chr1.4:20538251-20538270 MS.gene052832:intron 30.0%
! TCCAAAAGGAAATTTTCTTG+AGG - chr1.4:20538721-20538740 MS.gene052832:intron 30.0%
! TGATAGTGTTTCTCTATTGA+TGG - chr1.4:20536485-20536504 MS.gene052832:CDS 30.0%
!! AATATCAAAGCAGTTGATGA+AGG - chr1.4:20537412-20537431 MS.gene052832:CDS 30.0%
!! CAATAAGAGTTGGTAATGAA+AGG - chr1.4:20538326-20538345 MS.gene052832:CDS 30.0%
!! CATTGGTGGATCATATTTTA+AGG - chr1.4:20537348-20537367 MS.gene052832:CDS 30.0%
!! TCCTTAAGTTGATTGATGAA+CGG - chr1.4:20539453-20539472 MS.gene052832:intron 30.0%
!! TGTTTGCAATAATGGATTCT+TGG + chr1.4:20539533-20539552 None:intergenic 30.0%
!! TTTTGTTATGCATGTACAGA+AGG - chr1.4:20539105-20539124 MS.gene052832:intron 30.0%
!!! ATTTTTTGTTCAACACCGTT+TGG + chr1.4:20537232-20537251 None:intergenic 30.0%
!!! GTTGCAATTTTTTGTCTGAA+AGG - chr1.4:20537796-20537815 MS.gene052832:CDS 30.0%
AAAGAATGCAAAGTGGAGAT+TGG - chr1.4:20539419-20539438 MS.gene052832:intron 35.0%
AACAGCTTCATTAACAGCTT+CGG + chr1.4:20538117-20538136 None:intergenic 35.0%
ACCAAGGAAAACTCTTGTAT+GGG - chr1.4:20538379-20538398 MS.gene052832:CDS 35.0%
ACTTTCTTGAACTTCTTCTG+TGG + chr1.4:20537479-20537498 None:intergenic 35.0%
AGTGATAGACCTAACTTATC+CGG - chr1.4:20538985-20539004 MS.gene052832:intron 35.0%
ATGACACAATCTTACGTCAA+TGG - chr1.4:20538164-20538183 MS.gene052832:intron 35.0%
CAACACCGTTTGGAAATTTA+AGG + chr1.4:20537222-20537241 None:intergenic 35.0%
CCAAAGGAGTTTATGAAAAG+AGG - chr1.4:20539281-20539300 MS.gene052832:intron 35.0%
CTGAATTTCTTTGTTGCAGA+GGG - chr1.4:20537144-20537163 MS.gene052832:intron 35.0%
GATGAAGCATAAGACATTCA+AGG - chr1.4:20539137-20539156 MS.gene052832:intron 35.0%
GTAACCACTGTTTCAAATCA+TGG + chr1.4:20538258-20538277 None:intergenic 35.0%
GTATTTGTAGAGTGTACCAA+AGG - chr1.4:20539265-20539284 MS.gene052832:intron 35.0%
GTGTTTGATGTTTCAATTGC+AGG - chr1.4:20539482-20539501 MS.gene052832:intron 35.0%
GTTGTAAAGATGGAAGACAT+TGG - chr1.4:20538680-20538699 MS.gene052832:intron 35.0%
TACCAAGGAAAACTCTTGTA+TGG - chr1.4:20538378-20538397 MS.gene052832:CDS 35.0%
TCCGTTCATCAATCAACTTA+AGG + chr1.4:20539457-20539476 None:intergenic 35.0%
TCTGAATTTCTTTGTTGCAG+AGG - chr1.4:20537143-20537162 MS.gene052832:intron 35.0%
TGGAGAGTAATGGTGATATT+TGG - chr1.4:20537260-20537279 MS.gene052832:intron 35.0%
TGGCAAAAGAAAAGCAAGTA+TGG - chr1.4:20537609-20537628 MS.gene052832:intron 35.0%
TGTTCTCTTGAAAACTCACT+TGG + chr1.4:20538280-20538299 None:intergenic 35.0%
! CCCTCAAGAAAATTTCCTTT+TGG + chr1.4:20538725-20538744 None:intergenic 35.0%
! CCTCTTTTCATAAACTCCTT+TGG + chr1.4:20539284-20539303 None:intergenic 35.0%
! GAGTTCTGATGAAGTTGAAA+TGG - chr1.4:20537561-20537580 MS.gene052832:CDS 35.0%
! GCATAAGACATTCAAGGTTT+TGG - chr1.4:20539143-20539162 MS.gene052832:intron 35.0%
!! ATATTTTGGGTGGAGAGTAA+TGG - chr1.4:20537250-20537269 MS.gene052832:intron 35.0%
!! TGCTTTTCTTTTGCCATTTC+TGG + chr1.4:20537605-20537624 None:intergenic 35.0%
AAATGGCAAAGCAAGCTCAA+AGG - chr1.4:20537578-20537597 MS.gene052832:CDS 40.0%
AAGGAAAACTCTTGTATGGG+AGG - chr1.4:20538382-20538401 MS.gene052832:CDS 40.0%
ACTTCTATGAGCCATCCAAA+AGG - chr1.4:20538707-20538726 MS.gene052832:intron 40.0%
ACTTTCTTCACCTTCTTCTG+TGG + chr1.4:20537524-20537543 None:intergenic 40.0%
CCAAGCCCAAAGAAATTACT+AGG - chr1.4:20538520-20538539 MS.gene052832:intron 40.0%
CCTAGTAATTTCTTTGGGCT+TGG + chr1.4:20538523-20538542 None:intergenic 40.0%
CTTAGCAGGTAGTAACAAAG+TGG - chr1.4:20538058-20538077 MS.gene052832:intron 40.0%
CTTGGAGAACAAACAGCTAA+TGG + chr1.4:20539515-20539534 None:intergenic 40.0%
GAGGGTTCCTAAGATATATG+TGG - chr1.4:20537162-20537181 MS.gene052832:intron 40.0%
GAGGTGGTTGGAAAATATTC+AGG - chr1.4:20538401-20538420 MS.gene052832:CDS 40.0%
GATGCTAGTGAGTGAGTATT+GGG + chr1.4:20536959-20536978 None:intergenic 40.0%
GTCTTATGCTTCATCACATC+AGG + chr1.4:20539132-20539151 None:intergenic 40.0%
TCATCTTCATCCAACAGACA+TGG - chr1.4:20536324-20536343 MS.gene052832:CDS 40.0%
TCATTAACAGCTTCGGATGT+TGG + chr1.4:20538110-20538129 None:intergenic 40.0%
TGATGCTAGTGAGTGAGTAT+TGG + chr1.4:20536960-20536979 None:intergenic 40.0%
TTACGTCAATGGCTATCACT+TGG - chr1.4:20538175-20538194 MS.gene052832:intron 40.0%
! AATGAAAGGAGTGTGGAAGT+GGG - chr1.4:20538340-20538359 MS.gene052832:CDS 40.0%
! TAATGAAAGGAGTGTGGAAG+TGG - chr1.4:20538339-20538358 MS.gene052832:CDS 40.0%
! TCCCATACAAGAGTTTTCCT+TGG + chr1.4:20538383-20538402 None:intergenic 40.0%
! TTTCTTTGGGCTTGGTTTCT+TGG + chr1.4:20538515-20538534 None:intergenic 40.0%
!! AGTTGGTAATGAAAGGAGTG+TGG - chr1.4:20538333-20538352 MS.gene052832:CDS 40.0%
!! TTTTCTAGCCTTCTTCACCA+TGG + chr1.4:20538084-20538103 None:intergenic 40.0%
AGGTAGTAACAAAGTGGCCA+TGG - chr1.4:20538064-20538083 MS.gene052832:intron 45.0%
CAAGCTCAAAGGACCAGAAA+TGG - chr1.4:20537589-20537608 MS.gene052832:CDS 45.0%
CATGGCTGCAGATTCAGTAA+GGG - chr1.4:20539380-20539399 MS.gene052832:intron 45.0%
GAAAACTCTTGTATGGGAGG+TGG - chr1.4:20538385-20538404 MS.gene052832:CDS 45.0%
GATGATGAAGCCACAGAAGA+AGG - chr1.4:20537466-20537485 MS.gene052832:CDS 45.0%
GGGACTGCAACAATAAGAGT+TGG - chr1.4:20538316-20538335 MS.gene052832:CDS 45.0%
TCATGGCTGCAGATTCAGTA+AGG - chr1.4:20539379-20539398 MS.gene052832:intron 45.0%
TGAAAATGGGGAGGAAGTCA+TGG - chr1.4:20539327-20539346 MS.gene052832:intron 45.0%
TTGTGGAGTTGAAAATGGGG+AGG - chr1.4:20539318-20539337 MS.gene052832:intron 45.0%
!! AGTGAGTGAGTATTGGGCTA+AGG + chr1.4:20536953-20536972 None:intergenic 45.0%
!! TATGATAAAAATTATATATT+TGG - chr1.4:20537943-20537962 MS.gene052832:intron 5.0%
!!! CATATATATAATTTTTAATT+TGG - chr1.4:20538834-20538853 MS.gene052832:intron 5.0%
!!! TAAATTTTATAATTGTAAAT+GGG - chr1.4:20538771-20538790 MS.gene052832:intron 5.0%
!!! TTAAATTTTATAATTGTAAA+TGG - chr1.4:20538770-20538789 MS.gene052832:intron 5.0%
AACGACACTGCCATGTCTGT+TGG + chr1.4:20536337-20536356 None:intergenic 50.0%
ACTCTTGTATGGGAGGTGGT+TGG - chr1.4:20538389-20538408 MS.gene052832:CDS 50.0%
CAAAGTGGCCATGGTGAAGA+AGG - chr1.4:20538073-20538092 MS.gene052832:intron 50.0%
GAGCTTGGCTGAACTGCAAT+CGG + chr1.4:20536363-20536382 None:intergenic 50.0%
GCTGCAGATTCAGTAAGGGA+TGG - chr1.4:20539384-20539403 MS.gene052832:intron 50.0%
GGCTAAGGCTTAGAGAAGTG+AGG + chr1.4:20536938-20536957 None:intergenic 50.0%
TTTGGTGAAGAACACAGCCC+CGG - chr1.4:20539161-20539180 MS.gene052832:intron 50.0%
! ACTTGACTCGCCTTCTTCAG+TGG + chr1.4:20537446-20537465 None:intergenic 50.0%
! GGAGTGTGGAAGTGGGTTTA+AGG - chr1.4:20538347-20538366 MS.gene052832:CDS 50.0%
AGCCAAGCTCAGCGGCAATT+CGG - chr1.4:20536373-20536392 MS.gene052832:CDS 55.0%
AGCTCAGCGGCAATTCGGTA+AGG - chr1.4:20536378-20536397 MS.gene052832:CDS 55.0%
TACCGAATTGCCGCTGAGCT+TGG + chr1.4:20536378-20536397 None:intergenic 55.0%
TGCAGTTCAGCCAAGCTCAG+CGG - chr1.4:20536365-20536384 MS.gene052832:CDS 55.0%
! GGTGCTGAAGCCACTGAAGA+AGG - chr1.4:20537433-20537452 MS.gene052832:CDS 55.0%
GTGAAGAACACAGCCCCGGT+AGG - chr1.4:20539165-20539184 MS.gene052832:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 20536318 20539568 20536318 ID=MS.gene052832
chr1.4 mRNA 20536318 20539568 20536318 ID=MS.gene052832.t1;Parent=MS.gene052832
chr1.4 exon 20539492 20539568 20539492 ID=MS.gene052832.t1.exon1;Parent=MS.gene052832.t1
chr1.4 CDS 20539492 20539568 20539492 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20539371 20539395 20539371 ID=MS.gene052832.t1.exon2;Parent=MS.gene052832.t1
chr1.4 CDS 20539371 20539395 20539371 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20538261 20538516 20538261 ID=MS.gene052832.t1.exon3;Parent=MS.gene052832.t1
chr1.4 CDS 20538261 20538516 20538261 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20537690 20537820 20537690 ID=MS.gene052832.t1.exon4;Parent=MS.gene052832.t1
chr1.4 CDS 20537690 20537820 20537690 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20537345 20537615 20537345 ID=MS.gene052832.t1.exon5;Parent=MS.gene052832.t1
chr1.4 CDS 20537345 20537615 20537345 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20536704 20536762 20536704 ID=MS.gene052832.t1.exon6;Parent=MS.gene052832.t1
chr1.4 CDS 20536704 20536762 20536704 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
chr1.4 exon 20536318 20536611 20536318 ID=MS.gene052832.t1.exon7;Parent=MS.gene052832.t1
chr1.4 CDS 20536318 20536611 20536318 ID=cds.MS.gene052832.t1;Parent=MS.gene052832.t1
Gene Sequence

>MS.gene052832

ATGTCTTCATCTTCATCCAACAGACATGGCAGTGTCGTTGCTCCGATTGCAGTTCAGCCAAGCTCAGCGGCAATTCGGTAAGGAAACGCTGATTTATGTAATTCTTCAATTTGGTTTCTGTGATACTGTTTTTGAACTGATTTTTGGGTTAATTGAGCAATTTTCTGTGATAGTGTTTCTCTATTGATGGATTTGTCTGTAAGTATGATATTAGAATCTATTTGATTGAGATTAATAAACAAATTCAAAAAAAACTGAAAGAATAGGTTCAATGGATATTATAGTTAGGAGTTTCTAGTTTGTCGATGAAATGTTCTTAGCATTGCATATGCAATGTTGAGAGATTGATTCTGTGTGTTCTGCATGTGAAATAGACACTTAACAAAGTATGACAGATGCAGTTGTGATGATTCACAAATAGGACAAAGCAAATCCAACATAAACTATATATATAGAAAAAGAATATAGACATTAAAAATGTTTATATTTGCAATTTGTGATTTTGGAATATAGACATTATGTTTCATTTATAATGTATTATTTATGCATCATTGAGCCTGATATATATAGAAAAAGAATATAGACATTAAAACAGACTGAACACTCAAACTCTTTCACCTCACTTCTCTAAGCCTTAGCCCAATACTCACTCACTAGCATCAGTTAACAATGTCATTTCAACATGTTCCAGATTTTCATAGGATAATTTTGCAAGATAAGAAACTTGTAAGCATATTAGAAAGAATTATTTTGCATATTTACATTATGTTTCATTTATAATGTATTATTTATGCATCATTGTTTTAAAAATGTAAATGTATGATTTCTGAATTTCTTTGTTGCAGAGGGTTCCTAAGATATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTTCCAAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAGTTTTGCAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAATTATGCAAATATCAAAGCAGTTGATGAAGGTGCTGAAGCCACTGAAGAAGGCGAGTCAAGTGATGATGAAGCCACAGAAGAAGTTCAAGAAAGTGATGAATCAAGTGATGATGAAGCCACAGAAGAAGGTGAAGAAAGTGATGAAGAGAGTGATGAGAGTTCTGATGAAGTTGAAATGGCAAAGCAAGCTCAAAGGACCAGAAATGGCAAAAGAAAAGCAAGTATGGATTTTGACACTACCAAACAAAATTTCTCAGGGATTTTTCTCTGTTTTTTTTAGCTAGAAATTACTCATTTGGTAGACTAAGGTTTGTCTGTTACTCAATTTTTAAAATAAATCATTTGTTTTATAGAATAGAAATAATTACTTTTAAGACTTATTCAATGGTTTGTTGCAATTTTTTGTCTGAAAGGTTGCCTTGAAGTGCATAAATTATGAAGCAGATTTAGTTGTTTTTATTTTCAGATAATTTTTGGTAGATTCTTTTTATATAAAAATATGTGACTTTTTTTGGTAGGAAAAATATGTGATTTTTTTTATGATAAAAATTATATATTTGGTTTTTCGTAAAATCATCTTAAAATAATCTCTACATCAAATGTATAATAAATAGTTAATGCCTAAACAAAATAGATTATTATATCTCTTTAAACTTAGCAGGTAGTAACAAAGTGGCCATGGTGAAGAAGGCTAGAAAATATCCAACATCCGAAGCTGTTAATGAAGCTGTTAATGAGAATCCCTCTTTTCAAGTTATAATGACACAATCTTACGTCAATGGCTATCACTTGGTAAGTTTGCTATACAAAGTATCTATTAATTTTAGTTTTAATTAATATCTCTGATAATCCATGATTTGAAACAGTGGTTACCAAGTGAGTTTTCAAGAGAACACTTGAAAGATTTTAAAGGGACTGCAACAATAAGAGTTGGTAATGAAAGGAGTGTGGAAGTGGGTTTAAGGTATTATGATACCAAGGAAAACTCTTGTATGGGAGGTGGTTGGAAAATATTCAGGCAAAAATATAATTTGCAAGTTGATGATGTTTGTGAATTTGAGATGATTCAAAGAAGACCAATTTTATTCATTGTTACTATTACTCGTGCCAAGAAACCAAGCCCAAAGAAATTACTAGGTGTTTCTTTCTTTCTTTTTTATTTGTTTTTTTTGTATGTTATAATTTTTTTTATTTGATATTTGATTAAAATCTATTTTTCTTTATTTCTTTTGTCCATGATAAGGTTATAAAAAAGGGGTCTCTTGTCATAGTAGTGTTGTAAAGATGGAAGACATTGGAGAAACTTCTATGAGCCATCCAAAAGGAAATTTTCTTGAGGGTATTCTTCTAACTTTTTATAGTTTTTTAAATTTTATAATTGTAAATGGGTTTGAAATATTTGTAACTTTTAAATAGTTCTTTGAACTATCATATATATAATTTTTAATTTGGTCTTCAAACTCATGAATAACTTATTAGTTTTTGGACTAATCTTTTAATTGTATATCCTTAATCTTAAATTTAAAATACCCGTGAATTTGAAATAATTTTAATTTATCTTGTTGAACTTTTTGTTAGTTAGTGATAGACCTAACTTATCCGGTATTCAATTTTTTCTGTTAGTTTAGAACCTAACTATAAATCTATTCAAATACATATAATTTTTTTGGGTGTTAAAAAAATTATATTAGATACTATCATTTTGTTATGCATGTACAGAAGGTCCTGATGTGATGAAGCATAAGACATTCAAGGTTTTGGTGAAGAACACAGCCCCGGTAGGATTAATTATATAAAATATAGTAACATATATAGATAGAGATGTAAGAACTACAAAATAAATAAATGATATTTTTGTTTGTATTTGTAGAGTGTACCAAAGGAGTTTATGAAAAGAGGATGTCATGAAAATATTGTGGAGTTGAAAATGGGGAGGAAGTCATGGTTTGTAAAAGTGAATTATTATGAGAGTATTCATGGCTGCAGATTCAGTAAGGGATGGAGAAAATTTATGAAAGAATGCAAAGTGGAGATTGGAGATACTTGTCTCCTTAAGTTGATTGATGAACGGAAATTTGTGTTTGATGTTTCAATTGCAGGAAAGAATCCATTAGCTGTTTGTTCTCCAAGAATCCATTATTGCAAACATCTGTATTTCATTTGA
Protein sequence

>MS.gene052832.t1

MSSSSSNRHGSVVAPIAVQPSSAAIRVSLLMDLSVKIFGANTLEINYANIKAVDEGAEATEEGESSDDEATEEVQESDESSDDEATEEGEESDEESDESSDEVEMAKQAQRTRNGKRKASSNKVAMVKKARKYPTSEAVNEAVNENPSFQVIMTQSYVNGYHLWLPSEFSREHLKDFKGTATIRVGNERSVEVGLRYYDTKENSCMGGGWKIFRQKYNLQVDDVCEFEMIQRRPILFIVTITRAKKPSPKKLLEGPDVMKHKTFKVLVKNTAPSVPKEFMKRGCHENIVELKMGRKSWFVKVNYYESIHGCRFSKGWRKFMKECKVEIGDTCLLKLIDERKFVFDVSIAGKNPLAVCSPRIHYCKHLYFI