AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99290.t1 MTR_5g044670 89.091 330 36 0 1 330 1 330 0.0 613
MS.gene99290.t1 MTR_3g098790 32.248 307 183 8 16 310 13 306 1.07e-45 158
MS.gene99290.t1 MTR_8g103940 28.955 335 186 6 2 309 28 337 3.59e-45 160
MS.gene99290.t1 MTR_7g050710 31.715 309 182 9 16 306 13 310 1.15e-44 155
MS.gene99290.t1 MTR_7g050580 33.557 298 174 7 16 306 13 293 2.92e-44 154
MS.gene99290.t1 MTR_3g061350 32.423 293 170 9 16 301 12 283 1.67e-42 149
MS.gene99290.t1 MTR_7g050720 31.875 320 176 11 16 305 10 317 5.27e-42 148
MS.gene99290.t1 MTR_7g050560 30.492 305 182 8 16 310 13 297 9.80e-42 147
MS.gene99290.t1 MTR_1g108780 30.159 315 170 9 32 318 13 305 1.24e-41 150
MS.gene99290.t1 MTR_1g108780 23.549 293 186 11 16 304 201 459 7.08e-12 66.2
MS.gene99290.t1 MTR_7g050590 30.303 297 180 7 31 310 1 287 4.58e-41 145
MS.gene99290.t1 MTR_1g035640 32.302 291 163 9 25 304 14 281 3.53e-40 147
MS.gene99290.t1 MTR_3g061370 29.720 286 187 5 16 301 9 280 6.89e-40 142
MS.gene99290.t1 MTR_3g061310 30.847 295 173 7 16 301 12 284 1.54e-39 141
MS.gene99290.t1 MTR_3g061340 28.475 295 171 10 16 302 12 274 5.84e-38 136
MS.gene99290.t1 MTR_3g061320 29.371 286 183 5 16 301 12 278 9.46e-37 133
MS.gene99290.t1 MTR_6g061070 29.096 354 206 10 5 325 3 344 2.69e-36 136
MS.gene99290.t1 MTR_1g035570 30.584 291 181 7 25 307 14 291 1.87e-35 133
MS.gene99290.t1 MTR_4g119990 29.167 336 203 11 1 314 1 323 5.55e-35 131
MS.gene99290.t1 MTR_3g061330 29.961 257 160 8 16 269 12 251 9.29e-35 127
MS.gene99290.t1 MTR_1g035620 31.023 303 176 8 10 304 3 280 2.24e-33 127
MS.gene99290.t1 MTR_6g043890 29.260 311 178 9 1 289 1 291 1.06e-32 124
MS.gene99290.t1 MTR_1g035460 27.302 315 185 8 31 316 1 300 1.28e-32 125
MS.gene99290.t1 MTR_1g050845 28.904 301 173 7 10 304 3 268 3.69e-31 124
MS.gene99290.t1 MTR_3g005490 27.027 296 163 8 13 306 18 262 1.44e-28 111
MS.gene99290.t1 MTR_3g005460 29.000 300 163 8 16 306 262 520 1.48e-28 115
MS.gene99290.t1 MTR_3g005460 23.765 324 174 10 2 319 12 268 9.06e-19 87.4
MS.gene99290.t1 MTR_3g005480 24.832 298 168 7 13 306 18 263 1.73e-27 108
MS.gene99290.t1 MTR_1g035520 28.344 314 178 9 25 302 81 383 4.73e-27 109
MS.gene99290.t1 MTR_3g061290 35.404 161 86 4 16 169 12 161 8.56e-27 105
MS.gene99290.t1 MTR_3g005420 25.418 299 181 7 9 306 282 539 4.41e-26 108
MS.gene99290.t1 MTR_3g005420 32.800 125 82 2 13 136 37 160 5.67e-16 79.0
MS.gene99290.t1 MTR_5g070440 25.253 297 178 8 12 306 17 271 8.72e-26 104
MS.gene99290.t1 MTR_1g035480 40.299 134 74 3 16 149 17 144 7.31e-25 99.4
MS.gene99290.t1 MTR_7g050640 42.718 103 55 1 7 109 8 106 5.76e-23 92.4
MS.gene99290.t1 MTR_1g033740 35.821 134 79 3 16 149 22 148 2.17e-22 93.2
MS.gene99290.t1 MTR_1g034240 35.156 128 83 0 16 143 7 134 2.71e-22 97.1
MS.gene99290.t1 MTR_1g034240 29.730 148 86 5 178 310 255 399 9.29e-12 65.9
MS.gene99290.t1 MTR_1g021360 24.399 291 199 7 27 306 193 473 5.77e-22 96.7
MS.gene99290.t1 MTR_4g068320 32.275 189 114 3 13 191 3 187 7.13e-22 95.9
MS.gene99290.t1 MTR_4g068320 31.818 110 69 3 200 306 285 391 6.22e-12 66.6
MS.gene99290.t1 MTR_1g035650 29.148 223 128 5 10 224 3 203 7.52e-22 92.0
MS.gene99290.t1 MTR_3g005495 33.577 137 90 1 9 145 236 371 3.11e-21 94.4
MS.gene99290.t1 MTR_3g005495 33.735 83 55 0 32 114 29 111 1.08e-14 75.1
MS.gene99290.t1 MTR_1g035500 35.115 131 79 3 16 146 25 149 3.85e-20 87.0
MS.gene99290.t1 MTR_1g021400 24.773 331 208 12 16 309 30 356 1.30e-19 89.0
MS.gene99290.t1 MTR_1g034210 39.796 98 59 0 16 113 7 104 1.53e-19 88.2
MS.gene99290.t1 MTR_1g034210 39.796 98 59 0 16 113 7 104 2.92e-19 87.8
MS.gene99290.t1 MTR_1g035510 40.146 137 73 5 31 167 1 128 3.46e-19 85.1
MS.gene99290.t1 MTR_1g021330 23.214 336 206 8 17 302 26 359 8.05e-19 86.7
MS.gene99290.t1 MTR_1g035470 38.793 116 64 4 31 146 1 109 2.15e-18 80.9
MS.gene99290.t1 MTR_3g061220 34.746 118 60 3 16 133 12 112 2.77e-18 79.7
MS.gene99290.t1 MTR_1g035440 38.000 100 61 1 16 114 15 114 4.12e-16 77.0
MS.gene99290.t1 MTR_3g005420 32.800 125 82 2 13 136 18 141 5.12e-16 79.0
MS.gene99290.t1 MTR_1g021380 25.000 316 197 10 17 301 22 328 3.88e-15 75.5
MS.gene99290.t1 MTR_3g005440 36.471 85 54 0 30 114 34 118 4.57e-15 75.9
MS.gene99290.t1 MTR_3g005440 32.407 108 72 1 20 127 246 352 4.58e-14 73.2
MS.gene99290.t1 MTR_1g021410 23.385 325 207 8 17 302 23 344 4.58e-15 75.5
MS.gene99290.t1 MTR_1g098450 22.936 327 187 13 16 316 12 299 7.02e-15 74.7
MS.gene99290.t1 MTR_1g021435 23.867 331 193 11 23 301 29 352 2.10e-14 73.6
MS.gene99290.t1 MTR_1g035490 33.945 109 69 2 205 311 97 204 3.28e-14 72.8
MS.gene99290.t1 MTR_1g021440 37.778 90 56 0 17 106 23 112 4.35e-14 72.0
MS.gene99290.t1 MTR_1g021440 37.778 90 56 0 17 106 23 112 4.80e-14 71.6
MS.gene99290.t1 MTR_1g021440 37.778 90 56 0 17 106 23 112 9.71e-14 72.0
MS.gene99290.t1 MTR_1g021440 37.778 90 56 0 17 106 23 112 1.10e-13 71.6
MS.gene99290.t1 MTR_1g021320 41.250 80 47 0 27 106 193 272 4.18e-13 70.5
MS.gene99290.t1 MTR_1g021320 36.667 90 57 0 16 105 25 114 1.35e-12 68.9
MS.gene99290.t1 MTR_1g021350 34.043 94 62 0 17 110 22 115 3.02e-12 67.0
MS.gene99290.t1 MTR_1g021290 35.556 90 57 1 17 106 157 245 3.73e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99290.t1 AT3G18990 29.446 343 181 10 16 306 6 339 8.30e-37 136
MS.gene99290.t1 AT3G18990 29.155 343 182 10 16 306 6 339 1.49e-36 134
MS.gene99290.t1 AT4G01580 38.095 147 75 4 16 161 30 161 5.04e-22 92.0
MS.gene99290.t1 AT3G18960 41.414 99 58 0 16 114 30 128 1.16e-19 85.9
MS.gene99290.t1 AT3G18960 41.414 99 58 0 16 114 30 128 1.60e-19 85.9
MS.gene99290.t1 AT1G49475 33.974 156 92 3 16 166 34 183 7.83e-19 83.6
MS.gene99290.t1 AT3G18960 42.169 83 48 0 32 114 9 91 4.04e-15 72.8
MS.gene99290.t1 AT4G33280 21.918 292 207 7 30 306 37 322 1.28e-14 73.9
MS.gene99290.t1 AT1G49480 32.727 110 67 3 200 306 119 224 5.86e-13 67.8
MS.gene99290.t1 AT1G49480 32.727 110 67 3 200 306 119 224 5.86e-13 67.8
MS.gene99290.t1 AT1G49480 32.727 110 67 3 200 306 119 224 5.86e-13 67.8
MS.gene99290.t1 AT5G09780 23.203 306 204 11 11 299 14 305 6.71e-13 68.9

Find 55 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACCAAATGGTGATAAA+TGG 0.158299 5.4:+34127891 MS.gene99290:CDS
AGCATTGAACTTGAAGTTTA+AGG 0.206027 5.4:-34128901 None:intergenic
CTTTCCATTCAAATACTCTT+TGG 0.236141 5.4:-34128832 None:intergenic
GTGATAAATGGGAAGTAAAT+TGG 0.244779 5.4:+34127903 MS.gene99290:CDS
GCGCTTTGGAAATGGATTAT+TGG 0.263628 5.4:+34128062 MS.gene99290:CDS
AGACGATTATATTGAGATTT+CGG 0.264534 5.4:+34128159 MS.gene99290:CDS
ATGCAACGGACGAATGAAAA+AGG 0.295165 5.4:-34128518 None:intergenic
TTTGGTAGAACTAGGGTTAC+TGG 0.318076 5.4:-34127878 None:intergenic
AAACACTACAAAATACTAAA+AGG 0.340468 5.4:+34128467 MS.gene99290:CDS
TTTCTTTCAACTTGATCTCT+AGG 0.366619 5.4:-34128097 None:intergenic
CATCAAATGAAGCTATGAAT+TGG 0.370847 5.4:+34128440 MS.gene99290:CDS
TCAACTTGATCTCTAGGGCT+TGG 0.372702 5.4:-34128091 None:intergenic
GATATTTGACAAAAGCGCTT+TGG 0.378554 5.4:+34128048 MS.gene99290:CDS
CAACACTATTCAATGAGTTA+TGG 0.379815 5.4:+34127977 MS.gene99290:CDS
TGACAAAAGCGCTTTGGAAA+TGG 0.381408 5.4:+34128054 MS.gene99290:CDS
AACACTATTCAATGAGTTAT+GGG 0.394195 5.4:+34127978 MS.gene99290:CDS
CACGACGTGTGGTTTCAAAA+TGG 0.395330 5.4:+34127938 MS.gene99290:CDS
CAATGTAACAGATGCTTCCT+AGG 0.399104 5.4:+34127819 MS.gene99290:intron
TTTACAATTTCAGGAGGTAA+TGG 0.402632 5.4:+34128388 MS.gene99290:intron
TCTAGCTCCGCGCACTTTCA+AGG 0.407616 5.4:+34129068 MS.gene99290:CDS
TCCATCCTACCTTGAACATA+TGG 0.422875 5.4:+34128540 MS.gene99290:CDS
ACTTCCCATTTATCACCATT+TGG 0.427050 5.4:-34127896 None:intergenic
GGGATTGCAACCGTGGTGTT+TGG 0.435175 5.4:+34128858 MS.gene99290:CDS
AGTTATGAGTGCTCGATTAG+TGG 0.441965 5.4:-34128925 None:intergenic
TCTACCAAATGGTGATAAAT+GGG 0.445463 5.4:+34127892 MS.gene99290:CDS
TTCTTTCAACTTGATCTCTA+GGG 0.452245 5.4:-34128096 None:intergenic
GAGTATTTGAATGGAAAGGA+TGG 0.463140 5.4:+34128837 MS.gene99290:CDS
AATCGAGCACTCATAACTTC+TGG 0.465532 5.4:+34128930 MS.gene99290:CDS
TATCACCATTTGGTAGAACT+AGG 0.471668 5.4:-34127886 None:intergenic
CGTGTGGTTTCAAAATGGTT+GGG 0.485615 5.4:+34127943 MS.gene99290:CDS
GTTATCGGAATGAAAATTCA+TGG 0.486333 5.4:-34128494 None:intergenic
CAAAGAGTATTTGAATGGAA+AGG 0.500378 5.4:+34128833 MS.gene99290:CDS
ACGTGTGGTTTCAAAATGGT+TGG 0.508556 5.4:+34127942 MS.gene99290:CDS
TACTCTTTGGAAAACGATGT+TGG 0.517680 5.4:-34128819 None:intergenic
TATTTCTTCACAAAACTCCT+AGG 0.519057 5.4:-34127836 None:intergenic
TTGGTAGAACTAGGGTTACT+GGG 0.523068 5.4:-34127877 None:intergenic
ATCACCATTTGGTAGAACTA+GGG 0.529841 5.4:-34127885 None:intergenic
ACCATATGTTCAAGGTAGGA+TGG 0.542632 5.4:-34128541 None:intergenic
GTAACCCTAGTTCTACCAAA+TGG 0.543476 5.4:+34127881 MS.gene99290:CDS
ATATAGATACCATATGTTCA+AGG 0.544245 5.4:-34128549 None:intergenic
AGTATTTGAATGGAAAGGAT+GGG 0.554047 5.4:+34128838 MS.gene99290:CDS
CGCGGAGCTAGATTGATCAC+TGG 0.561180 5.4:-34129057 None:intergenic
AACCGTGGTGTTTGGTGATG+AGG 0.573245 5.4:+34128866 MS.gene99290:CDS
CTGGAAATCCTAAAATGAAG+AGG 0.579411 5.4:+34129337 MS.gene99290:CDS
GAGCACTCATAACTTCTGGT+TGG 0.583650 5.4:+34128934 MS.gene99290:CDS
AAAAGAATGAAAACTCGTGA+TGG 0.588738 5.4:+34128223 MS.gene99290:CDS
TAAAACGTGACCACGACGTG+TGG 0.590381 5.4:+34127927 MS.gene99290:CDS
AGATACCATATGTTCAAGGT+AGG 0.608884 5.4:-34128545 None:intergenic
TGGAACTGATGAACAACACT+CGG 0.613370 5.4:-34127599 None:intergenic
TTTAATACCTTGAAAGTGCG+CGG 0.633523 5.4:-34129075 None:intergenic
CATGAAGATGCTGAAAAGAG+TGG 0.644431 5.4:+34128130 MS.gene99290:CDS
TCAAGGTAGGATGGATGCAA+CGG 0.653476 5.4:-34128532 None:intergenic
AAAGGATGGGATTGCAACCG+TGG 0.682554 5.4:+34128851 MS.gene99290:CDS
ATCCTCATCACCAAACACCA+CGG 0.689007 5.4:-34128868 None:intergenic
TTGGTGATGAGGATAAGACT+TGG 0.724527 5.4:+34128877 MS.gene99290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAGAAAATTAAATAAA+AGG - chr5.4:34127698-34127717 None:intergenic 10.0%
!! ATTAATTAAACTAAAAAGAA+TGG - chr5.4:34128664-34128683 None:intergenic 10.0%
!! TTAATGAATTTATTTACTTT+CGG + chr5.4:34128679-34128698 MS.gene99290:intron 10.0%
!!! ATTTTCTACTTTAATATTTT+TGG + chr5.4:34127706-34127725 MS.gene99290:intron 10.0%
!! AAGAAAAAATATCAAGATTT+AGG - chr5.4:34128769-34128788 None:intergenic 15.0%
!!! AGTATTTTTTTTACAATTTC+AGG + chr5.4:34128379-34128398 MS.gene99290:intron 15.0%
!! AAACACTACAAAATACTAAA+AGG + chr5.4:34128467-34128486 MS.gene99290:CDS 20.0%
!! AAAGAAGGAAAAAATTAATG+TGG + chr5.4:34128267-34128286 MS.gene99290:intron 20.0%
!! AACCATTTGCATATAAAAAA+TGG - chr5.4:34129153-34129172 None:intergenic 20.0%
!! AAGGAATACATGAATTATAT+AGG - chr5.4:34128640-34128659 None:intergenic 20.0%
!! AATGTGGATAAAAAAATCAA+AGG + chr5.4:34128283-34128302 MS.gene99290:intron 20.0%
!! TATGAATGTGTCTAAATAAA+AGG - chr5.4:34127738-34127757 None:intergenic 20.0%
!! TCTTGTGTTTAAATGATAAT+TGG + chr5.4:34128785-34128804 MS.gene99290:intron 20.0%
!! TTAAACTAAAAAGAATGGAA+AGG - chr5.4:34128659-34128678 None:intergenic 20.0%
!! TTAAACTAGATAGTAAAATG+TGG - chr5.4:34128600-34128619 None:intergenic 20.0%
!!! ATTTTTTTTACAATTTCAGG+AGG + chr5.4:34128382-34128401 MS.gene99290:intron 20.0%
!!! CATTAATTTTTTCCTTCTTT+AGG - chr5.4:34128267-34128286 None:intergenic 20.0%
!!! GAAGAATATAATTTTGAAGT+TGG + chr5.4:34128969-34128988 MS.gene99290:CDS 20.0%
!!! TACAATCTCTTTCTTTTTAA+GGG - chr5.4:34127663-34127682 None:intergenic 20.0%
!!! TAGTTTTTAGAATTGAAACA+AGG + chr5.4:34128008-34128027 MS.gene99290:CDS 20.0%
!!! TTACAATCTCTTTCTTTTTA+AGG - chr5.4:34127664-34127683 None:intergenic 20.0%
!!! TTCCATTTTTTATATGCAAA+TGG + chr5.4:34129148-34129167 MS.gene99290:intron 20.0%
!!! TTTTTTTTTAGTGCTATTGT+TGG + chr5.4:34127761-34127780 MS.gene99290:intron 20.0%
! AACACTATTCAATGAGTTAT+GGG + chr5.4:34127978-34127997 MS.gene99290:CDS 25.0%
! ACAACATGATTTATAGTAGA+CGG - chr5.4:34129203-34129222 None:intergenic 25.0%
! AGACGATTATATTGAGATTT+CGG + chr5.4:34128159-34128178 MS.gene99290:CDS 25.0%
! ATATAGATACCATATGTTCA+AGG - chr5.4:34128552-34128571 None:intergenic 25.0%
! GAGGAGAATAATTAAAATGA+AGG - chr5.4:34128325-34128344 None:intergenic 25.0%
! GTAGCAATTTAACATTATGA+TGG - chr5.4:34128347-34128366 None:intergenic 25.0%
! TATTTACTTTCGGTATATCT+AGG + chr5.4:34128689-34128708 MS.gene99290:intron 25.0%
! TGAAAAAACATTGTGACTTA+TGG - chr5.4:34127622-34127641 None:intergenic 25.0%
! TTTATATGCAAATGGTTGAA+AGG + chr5.4:34129156-34129175 MS.gene99290:intron 25.0%
!! ATAGAGTTTTTCTTTCGTTA+CGG + chr5.4:34129251-34129270 MS.gene99290:intron 25.0%
!! ATGTGGAAATTGATTCTAAA+AGG - chr5.4:34128583-34128602 None:intergenic 25.0%
!! TTTGATATCCTCTTCATTTT+AGG - chr5.4:34129348-34129367 None:intergenic 25.0%
!!! TTTTCCAAAGAGTATTTGAA+TGG + chr5.4:34128828-34128847 MS.gene99290:CDS 25.0%
AAAAGAATGAAAACTCGTGA+TGG + chr5.4:34128223-34128242 MS.gene99290:CDS 30.0%
AGTATTTGAATGGAAAGGAT+GGG + chr5.4:34128838-34128857 MS.gene99290:CDS 30.0%
CAACACTATTCAATGAGTTA+TGG + chr5.4:34127977-34127996 MS.gene99290:CDS 30.0%
CAACATGATTTATAGTAGAC+GGG - chr5.4:34129202-34129221 None:intergenic 30.0%
CATCAAATGAAGCTATGAAT+TGG + chr5.4:34128440-34128459 MS.gene99290:CDS 30.0%
CTTTCCATTCAAATACTCTT+TGG - chr5.4:34128835-34128854 None:intergenic 30.0%
GCAATTTAACATTATGATGG+AGG - chr5.4:34128344-34128363 None:intergenic 30.0%
GTGATAAATGGGAAGTAAAT+TGG + chr5.4:34127903-34127922 MS.gene99290:CDS 30.0%
GTTATCGGAATGAAAATTCA+TGG - chr5.4:34128497-34128516 None:intergenic 30.0%
TATTTCTTCACAAAACTCCT+AGG - chr5.4:34127839-34127858 None:intergenic 30.0%
TCAAAGGAAAAACCTAAAGA+AGG + chr5.4:34128252-34128271 MS.gene99290:intron 30.0%
TCTACCAAATGGTGATAAAT+GGG + chr5.4:34127892-34127911 MS.gene99290:CDS 30.0%
TTCTACCAAATGGTGATAAA+TGG + chr5.4:34127891-34127910 MS.gene99290:CDS 30.0%
TTCTTTCAACTTGATCTCTA+GGG - chr5.4:34128099-34128118 None:intergenic 30.0%
TTTACAATTTCAGGAGGTAA+TGG + chr5.4:34128388-34128407 MS.gene99290:intron 30.0%
TTTCTTTCAACTTGATCTCT+AGG - chr5.4:34128100-34128119 None:intergenic 30.0%
! AGCATTGAACTTGAAGTTTA+AGG - chr5.4:34128904-34128923 None:intergenic 30.0%
!! AGGAGTTTTGTGAAGAAATA+TGG + chr5.4:34127839-34127858 MS.gene99290:CDS 30.0%
!! CAAAGAGTATTTGAATGGAA+AGG + chr5.4:34128833-34128852 MS.gene99290:CDS 30.0%
!! GGAGTTTTGTGAAGAAATAT+GGG + chr5.4:34127840-34127859 MS.gene99290:CDS 30.0%
!! TTTAGGTTTTTCCTTTGAGA+TGG - chr5.4:34128250-34128269 None:intergenic 30.0%
ACGAATGAAAAAGGGGTTAT+CGG - chr5.4:34128512-34128531 None:intergenic 35.0%
ACTTCCCATTTATCACCATT+TGG - chr5.4:34127899-34127918 None:intergenic 35.0%
AGATACCATATGTTCAAGGT+AGG - chr5.4:34128548-34128567 None:intergenic 35.0%
ATCACCATTTGGTAGAACTA+GGG - chr5.4:34127888-34127907 None:intergenic 35.0%
CTGGAAATCCTAAAATGAAG+AGG + chr5.4:34129337-34129356 MS.gene99290:CDS 35.0%
GAGTATTTGAATGGAAAGGA+TGG + chr5.4:34128837-34128856 MS.gene99290:CDS 35.0%
TATCACCATTTGGTAGAACT+AGG - chr5.4:34127889-34127908 None:intergenic 35.0%
! CGAGTTTTCATTCTTTTCTG+AGG - chr5.4:34128220-34128239 None:intergenic 35.0%
! TACTCTTTGGAAAACGATGT+TGG - chr5.4:34128822-34128841 None:intergenic 35.0%
! TTTAATACCTTGAAAGTGCG+CGG - chr5.4:34129078-34129097 None:intergenic 35.0%
!! GATATTTGACAAAAGCGCTT+TGG + chr5.4:34128048-34128067 MS.gene99290:CDS 35.0%
AATCGAGCACTCATAACTTC+TGG + chr5.4:34128930-34128949 MS.gene99290:CDS 40.0%
ACCATATGTTCAAGGTAGGA+TGG - chr5.4:34128544-34128563 None:intergenic 40.0%
ACGTGTGGTTTCAAAATGGT+TGG + chr5.4:34127942-34127961 MS.gene99290:CDS 40.0%
AGTTATGAGTGCTCGATTAG+TGG - chr5.4:34128928-34128947 None:intergenic 40.0%
ATGCAACGGACGAATGAAAA+AGG - chr5.4:34128521-34128540 None:intergenic 40.0%
CAATGTAACAGATGCTTCCT+AGG + chr5.4:34127819-34127838 MS.gene99290:intron 40.0%
CATGAAGATGCTGAAAAGAG+TGG + chr5.4:34128130-34128149 MS.gene99290:CDS 40.0%
CGTGTGGTTTCAAAATGGTT+GGG + chr5.4:34127943-34127962 MS.gene99290:CDS 40.0%
GTACGTATTCTTGCAGATTC+TGG + chr5.4:34129318-34129337 MS.gene99290:intron 40.0%
TCCATCCTACCTTGAACATA+TGG + chr5.4:34128540-34128559 MS.gene99290:CDS 40.0%
TGCAACGGACGAATGAAAAA+GGG - chr5.4:34128520-34128539 None:intergenic 40.0%
TGGAACTGATGAACAACACT+CGG - chr5.4:34127602-34127621 None:intergenic 40.0%
TTGGTAGAACTAGGGTTACT+GGG - chr5.4:34127880-34127899 None:intergenic 40.0%
TTTGGTAGAACTAGGGTTAC+TGG - chr5.4:34127881-34127900 None:intergenic 40.0%
!! GCGCTTTGGAAATGGATTAT+TGG + chr5.4:34128062-34128081 MS.gene99290:CDS 40.0%
!! GTAACCCTAGTTCTACCAAA+TGG + chr5.4:34127881-34127900 MS.gene99290:CDS 40.0%
!! TGACAAAAGCGCTTTGGAAA+TGG + chr5.4:34128054-34128073 MS.gene99290:CDS 40.0%
!! TTGGTGATGAGGATAAGACT+TGG + chr5.4:34128877-34128896 MS.gene99290:CDS 40.0%
!!! TTCATTCTTTTCTGAGGTCG+AGG - chr5.4:34128214-34128233 None:intergenic 40.0%
ATCCTCATCACCAAACACCA+CGG - chr5.4:34128871-34128890 None:intergenic 45.0%
CACGACGTGTGGTTTCAAAA+TGG + chr5.4:34127938-34127957 MS.gene99290:CDS 45.0%
GAGCACTCATAACTTCTGGT+TGG + chr5.4:34128934-34128953 MS.gene99290:CDS 45.0%
GCAACGGACGAATGAAAAAG+GGG - chr5.4:34128519-34128538 None:intergenic 45.0%
TCAAGGTAGGATGGATGCAA+CGG - chr5.4:34128535-34128554 None:intergenic 45.0%
!! TCAACTTGATCTCTAGGGCT+TGG - chr5.4:34128094-34128113 None:intergenic 45.0%
!!! CATTTTGAAACCACACGTCG+TGG - chr5.4:34127940-34127959 None:intergenic 45.0%
AAAGGATGGGATTGCAACCG+TGG + chr5.4:34128851-34128870 MS.gene99290:CDS 50.0%
CTCGTGATGGTCCATCTCAA+AGG + chr5.4:34128236-34128255 MS.gene99290:intron 50.0%
TAAAACGTGACCACGACGTG+TGG + chr5.4:34127927-34127946 MS.gene99290:CDS 50.0%
!! AACCGTGGTGTTTGGTGATG+AGG + chr5.4:34128866-34128885 MS.gene99290:CDS 50.0%
CGCGGAGCTAGATTGATCAC+TGG - chr5.4:34129060-34129079 None:intergenic 55.0%
GGGATTGCAACCGTGGTGTT+TGG + chr5.4:34128858-34128877 MS.gene99290:CDS 55.0%
! TCTAGCTCCGCGCACTTTCA+AGG + chr5.4:34129068-34129087 MS.gene99290:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 34127590 34129383 34127590 ID=MS.gene99290
chr5.4 mRNA 34127590 34129383 34127590 ID=MS.gene99290.t1;Parent=MS.gene99290
chr5.4 exon 34127590 34127679 34127590 ID=MS.gene99290.t1.exon1;Parent=MS.gene99290.t1
chr5.4 CDS 34127590 34127679 34127590 ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4 exon 34127830 34128244 34127830 ID=MS.gene99290.t1.exon2;Parent=MS.gene99290.t1
chr5.4 CDS 34127830 34128244 34127830 ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4 exon 34128401 34128561 34128401 ID=MS.gene99290.t1.exon3;Parent=MS.gene99290.t1
chr5.4 CDS 34128401 34128561 34128401 ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4 exon 34128813 34129089 34128813 ID=MS.gene99290.t1.exon4;Parent=MS.gene99290.t1
chr5.4 CDS 34128813 34129089 34128813 ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4 exon 34129334 34129383 34129334 ID=MS.gene99290.t1.exon5;Parent=MS.gene99290.t1
chr5.4 CDS 34129334 34129383 34129334 ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
Gene Sequence

>MS.gene99290

ATGTCTTCTCCGAGTGTTGTTCATCAGTTCCATAAGTCACAATGTTTTTTCAAAGTCATCGTCGAGACAACCCTTAAAAAGAAAGAGATTGTAAGTGCATGTTAACCTTTTATTTAATTTTCTACTTTAATATTTTTGGTCACAACCTTTTATTTAGACACATTCATAGCTTTTTTTTTTAGTGCTATTGTTGGAGTAATAGTATAATTTTTTATTACTAAAATGTTTACAATGTAACAGATGCTTCCTAGGAGTTTTGTGAAGAAATATGGGAAAAAATTATCAAACCCAGTAACCCTAGTTCTACCAAATGGTGATAAATGGGAAGTAAATTGGATAAAACGTGACCACGACGTGTGGTTTCAAAATGGTTGGGAAAATTTTGCACAACACTATTCAATGAGTTATGGGCATTTTTTAGTTTTTAGAATTGAAACAAGGTCTCAATTTCAAGTAATGATATTTGACAAAAGCGCTTTGGAAATGGATTATTGGAGTATACCAAGCCCTAGAGATCAAGTTGAAAGAAACATTCATCATCATGAAGATGCTGAAAAGAGTGGCGATAAAGACGATTATATTGAGATTTCGGATGAGAGTGAAATGCAAAGATCTTCATCACCTCGACCTCAGAAAAGAATGAAAACTCGTGATGGTCCATCTCAAAGGAAAAACCTAAAGAAGGAAAAAATTAATGTGGATAAAAAAATCAAAGGAAAGTTATAATTTTCTCCTTCATTTTAATTATTCTCCTCCATCATAATGTTAAATTGCTACTTGAATGCTGAAAGTATTTTTTTTACAATTTCAGGAGGTAATGGAGAAAATTTCTTCAATGCAAAGAATGACTCATCAAATGAAGCTATGAATTGGAAAGAAACACTACAAAATACTAAAAGGAGAGCCATGAATTTTCATTCCGATAACCCCTTTTTCATTCGTCCGTTGCATCCATCCTACCTTGAACATATGGTATCTATATTGTTTATTCCTTTTAGAATCAATTTCCACATTTTACTATCTAGTTTAACAATCTAGAAATGTTTTCCTATATAATTCATGTATTCCTTTCCATTCTTTTTAGTTTAATTAATGAATTTATTTACTTTCGGTATATCTAGGATTTATTTTTATTCTTCTCAAACAACACAAGAGTATATAATAGTTAGATTTAAACCTAAATCTTGATATTTTTTCTTGTGTTTAAATGATAATTGGATCAGTTTATACCAACATCGTTTTCCAAAGAGTATTTGAATGGAAAGGATGGGATTGCAACCGTGGTGTTTGGTGATGAGGATAAGACTTGGTCCTTAAACTTCAAGTTCAATGCTACCACTAATCGAGCACTCATAACTTCTGGTTGGAGACAATTTGTCGAAGAATATAATTTTGAAGTTGGAGACACTTGTGTGTTTGAGATGACTGATAGTACAAATATCTTATTTAAAGTCTATATTGTTCGAGCCAGTGATCAATCTAGCTCCGCGCACTTTCAAGGTATTAAATAAATATATTATACGTTACAAATTATAATAAATAATTTACATATTTACGTTTCCATTTTTTATATGCAAATGGTTGAAAGGTTTAATATTTCTATGAGAAGCCCGTCTACTATAAATCATGTTGTGTTGCGATTATTTTTGTTATGTCACAATATAGAGTTTTTCTTTCGTTACGGTAAGTGTTATATTATTACATTCTTGCAAATAACATTACAAATGCGTACGTATTCTTGCAGATTCTGGAAATCCTAAAATGAAGAGGATATCAAATTCAAGACAAAACTGA
Protein sequence

>MS.gene99290.t1

MSSPSVVHQFHKSQCFFKVIVETTLKKKEIMLPRSFVKKYGKKLSNPVTLVLPNGDKWEVNWIKRDHDVWFQNGWENFAQHYSMSYGHFLVFRIETRSQFQVMIFDKSALEMDYWSIPSPRDQVERNIHHHEDAEKSGDKDDYIEISDESEMQRSSSPRPQKRMKTRDGGNGENFFNAKNDSSNEAMNWKETLQNTKRRAMNFHSDNPFFIRPLHPSYLEHMFIPTSFSKEYLNGKDGIATVVFGDEDKTWSLNFKFNATTNRALITSGWRQFVEEYNFEVGDTCVFEMTDSTNILFKVYIVRASDQSSSAHFQDSGNPKMKRISNSRQN