AlfalfaGEDB | MS.gene99290

M. sativa cultivar XinJiangDaYe / MS.gene99290

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99290.t1	AES97051.1	89.1	330	36	0	1	330	1	330	4.00E-167	597.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99290.t1	Q8L3W1	29.6	338	187	10	16	306	6	339	6.9e-27	122.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99290.t1	G7JXZ7	89.1	330	36	0	1	330	1	330	2.8e-167	597.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene99290.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene026334	MS.gene99290	PPI
MS.gene026340	MS.gene99290	PPI
MS.gene026341	MS.gene99290	PPI
MS.gene026344	MS.gene99290	PPI
MS.gene026345	MS.gene99290	PPI
MS.gene034709	MS.gene99290	PPI
MS.gene040686	MS.gene99290	PPI
MS.gene040687	MS.gene99290	PPI
MS.gene040688	MS.gene99290	PPI
MS.gene040691	MS.gene99290	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99290.t1	MTR_5g044670	89.091	330	36	0	1	330	1	330	0.0	613
MS.gene99290.t1	MTR_3g098790	32.248	307	183	8	16	310	13	306	1.07e-45	158
MS.gene99290.t1	MTR_8g103940	28.955	335	186	6	2	309	28	337	3.59e-45	160
MS.gene99290.t1	MTR_7g050710	31.715	309	182	9	16	306	13	310	1.15e-44	155
MS.gene99290.t1	MTR_7g050580	33.557	298	174	7	16	306	13	293	2.92e-44	154
MS.gene99290.t1	MTR_3g061350	32.423	293	170	9	16	301	12	283	1.67e-42	149
MS.gene99290.t1	MTR_7g050720	31.875	320	176	11	16	305	10	317	5.27e-42	148
MS.gene99290.t1	MTR_7g050560	30.492	305	182	8	16	310	13	297	9.80e-42	147
MS.gene99290.t1	MTR_1g108780	30.159	315	170	9	32	318	13	305	1.24e-41	150
MS.gene99290.t1	MTR_1g108780	23.549	293	186	11	16	304	201	459	7.08e-12	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99290.t1	AT3G18990	29.446	343	181	10	16	306	6	339	8.30e-37	136
MS.gene99290.t1	AT3G18990	29.155	343	182	10	16	306	6	339	1.49e-36	134
MS.gene99290.t1	AT4G01580	38.095	147	75	4	16	161	30	161	5.04e-22	92.0
MS.gene99290.t1	AT3G18960	41.414	99	58	0	16	114	30	128	1.16e-19	85.9
MS.gene99290.t1	AT3G18960	41.414	99	58	0	16	114	30	128	1.60e-19	85.9
MS.gene99290.t1	AT1G49475	33.974	156	92	3	16	166	34	183	7.83e-19	83.6
MS.gene99290.t1	AT3G18960	42.169	83	48	0	32	114	9	91	4.04e-15	72.8
MS.gene99290.t1	AT4G33280	21.918	292	207	7	30	306	37	322	1.28e-14	73.9
MS.gene99290.t1	AT1G49480	32.727	110	67	3	200	306	119	224	5.86e-13	67.8
MS.gene99290.t1	AT1G49480	32.727	110	67	3	200	306	119	224	5.86e-13	67.8

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Find 55 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATGAAAACTCGTGA+TGG	0.588738	5.4:+34128223	MS.gene99290:CDS
AAACACTACAAAATACTAAA+AGG	0.340468	5.4:+34128467	MS.gene99290:CDS
AAAGGATGGGATTGCAACCG+TGG	0.682554	5.4:+34128851	MS.gene99290:CDS
AACACTATTCAATGAGTTAT+GGG	0.394195	5.4:+34127978	MS.gene99290:CDS
AACCGTGGTGTTTGGTGATG+AGG	0.573245	5.4:+34128866	MS.gene99290:CDS
AATCGAGCACTCATAACTTC+TGG	0.465532	5.4:+34128930	MS.gene99290:CDS
ACCATATGTTCAAGGTAGGA+TGG	0.542632	5.4:-34128541	None:intergenic
ACGTGTGGTTTCAAAATGGT+TGG	0.508556	5.4:+34127942	MS.gene99290:CDS
ACTTCCCATTTATCACCATT+TGG	0.427050	5.4:-34127896	None:intergenic
AGACGATTATATTGAGATTT+CGG	0.264534	5.4:+34128159	MS.gene99290:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAATGAAAACTCGTGA+TGG	+	chr5.4:34128223-34128242	MS.gene99290:CDS	30.0%
AGTATTTGAATGGAAAGGAT+GGG	+	chr5.4:34128838-34128857	MS.gene99290:CDS	30.0%
CAACACTATTCAATGAGTTA+TGG	+	chr5.4:34127977-34127996	MS.gene99290:CDS	30.0%
CAACATGATTTATAGTAGAC+GGG	-	chr5.4:34129202-34129221	None:intergenic	30.0%
CATCAAATGAAGCTATGAAT+TGG	+	chr5.4:34128440-34128459	MS.gene99290:CDS	30.0%
CTTTCCATTCAAATACTCTT+TGG	-	chr5.4:34128835-34128854	None:intergenic	30.0%
GCAATTTAACATTATGATGG+AGG	-	chr5.4:34128344-34128363	None:intergenic	30.0%
GTGATAAATGGGAAGTAAAT+TGG	+	chr5.4:34127903-34127922	MS.gene99290:CDS	30.0%
GTTATCGGAATGAAAATTCA+TGG	-	chr5.4:34128497-34128516	None:intergenic	30.0%
TATTTCTTCACAAAACTCCT+AGG	-	chr5.4:34127839-34127858	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	34127590	34129383	34127590	ID=MS.gene99290
chr5.4	mRNA	34127590	34129383	34127590	ID=MS.gene99290.t1;Parent=MS.gene99290
chr5.4	exon	34127590	34127679	34127590	ID=MS.gene99290.t1.exon1;Parent=MS.gene99290.t1
chr5.4	CDS	34127590	34127679	34127590	ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4	exon	34127830	34128244	34127830	ID=MS.gene99290.t1.exon2;Parent=MS.gene99290.t1
chr5.4	CDS	34127830	34128244	34127830	ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4	exon	34128401	34128561	34128401	ID=MS.gene99290.t1.exon3;Parent=MS.gene99290.t1
chr5.4	CDS	34128401	34128561	34128401	ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4	exon	34128813	34129089	34128813	ID=MS.gene99290.t1.exon4;Parent=MS.gene99290.t1
chr5.4	CDS	34128813	34129089	34128813	ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1
chr5.4	exon	34129334	34129383	34129334	ID=MS.gene99290.t1.exon5;Parent=MS.gene99290.t1
chr5.4	CDS	34129334	34129383	34129334	ID=cds.MS.gene99290.t1;Parent=MS.gene99290.t1

Gene Sequence

>MS.gene99290

ATGTCTTCTCCGAGTGTTGTTCATCAGTTCCATAAGTCACAATGTTTTTTCAAAGTCATCGTCGAGACAACCCTTAAAAAGAAAGAGATTGTAAGTGCATGTTAACCTTTTATTTAATTTTCTACTTTAATATTTTTGGTCACAACCTTTTATTTAGACACATTCATAGCTTTTTTTTTTAGTGCTATTGTTGGAGTAATAGTATAATTTTTTATTACTAAAATGTTTACAATGTAACAGATGCTTCCTAGGAGTTTTGTGAAGAAATATGGGAAAAAATTATCAAACCCAGTAACCCTAGTTCTACCAAATGGTGATAAATGGGAAGTAAATTGGATAAAACGTGACCACGACGTGTGGTTTCAAAATGGTTGGGAAAATTTTGCACAACACTATTCAATGAGTTATGGGCATTTTTTAGTTTTTAGAATTGAAACAAGGTCTCAATTTCAAGTAATGATATTTGACAAAAGCGCTTTGGAAATGGATTATTGGAGTATACCAAGCCCTAGAGATCAAGTTGAAAGAAACATTCATCATCATGAAGATGCTGAAAAGAGTGGCGATAAAGACGATTATATTGAGATTTCGGATGAGAGTGAAATGCAAAGATCTTCATCACCTCGACCTCAGAAAAGAATGAAAACTCGTGATGGTCCATCTCAAAGGAAAAACCTAAAGAAGGAAAAAATTAATGTGGATAAAAAAATCAAAGGAAAGTTATAATTTTCTCCTTCATTTTAATTATTCTCCTCCATCATAATGTTAAATTGCTACTTGAATGCTGAAAGTATTTTTTTTACAATTTCAGGAGGTAATGGAGAAAATTTCTTCAATGCAAAGAATGACTCATCAAATGAAGCTATGAATTGGAAAGAAACACTACAAAATACTAAAAGGAGAGCCATGAATTTTCATTCCGATAACCCCTTTTTCATTCGTCCGTTGCATCCATCCTACCTTGAACATATGGTATCTATATTGTTTATTCCTTTTAGAATCAATTTCCACATTTTACTATCTAGTTTAACAATCTAGAAATGTTTTCCTATATAATTCATGTATTCCTTTCCATTCTTTTTAGTTTAATTAATGAATTTATTTACTTTCGGTATATCTAGGATTTATTTTTATTCTTCTCAAACAACACAAGAGTATATAATAGTTAGATTTAAACCTAAATCTTGATATTTTTTCTTGTGTTTAAATGATAATTGGATCAGTTTATACCAACATCGTTTTCCAAAGAGTATTTGAATGGAAAGGATGGGATTGCAACCGTGGTGTTTGGTGATGAGGATAAGACTTGGTCCTTAAACTTCAAGTTCAATGCTACCACTAATCGAGCACTCATAACTTCTGGTTGGAGACAATTTGTCGAAGAATATAATTTTGAAGTTGGAGACACTTGTGTGTTTGAGATGACTGATAGTACAAATATCTTATTTAAAGTCTATATTGTTCGAGCCAGTGATCAATCTAGCTCCGCGCACTTTCAAGGTATTAAATAAATATATTATACGTTACAAATTATAATAAATAATTTACATATTTACGTTTCCATTTTTTATATGCAAATGGTTGAAAGGTTTAATATTTCTATGAGAAGCCCGTCTACTATAAATCATGTTGTGTTGCGATTATTTTTGTTATGTCACAATATAGAGTTTTTCTTTCGTTACGGTAAGTGTTATATTATTACATTCTTGCAAATAACATTACAAATGCGTACGTATTCTTGCAGATTCTGGAAATCCTAAAATGAAGAGGATATCAAATTCAAGACAAAACTGA

Protein sequence

>MS.gene99290.t1

MSSPSVVHQFHKSQCFFKVIVETTLKKKEIMLPRSFVKKYGKKLSNPVTLVLPNGDKWEVNWIKRDHDVWFQNGWENFAQHYSMSYGHFLVFRIETRSQFQVMIFDKSALEMDYWSIPSPRDQVERNIHHHEDAEKSGDKDDYIEISDESEMQRSSSPRPQKRMKTRDGGNGENFFNAKNDSSNEAMNWKETLQNTKRRAMNFHSDNPFFIRPLHPSYLEHMFIPTSFSKEYLNGKDGIATVVFGDEDKTWSLNFKFNATTNRALITSGWRQFVEEYNFEVGDTCVFEMTDSTNILFKVYIVRASDQSSSAHFQDSGNPKMKRISNSRQN