AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073653

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073653.t1 MTR_1g035570 72.131 427 72 6 14 427 9 401 0.0 566
MS.gene073653.t1 MTR_1g035490 66.471 340 73 9 106 422 9 330 3.99e-133 386
MS.gene073653.t1 MTR_1g035640 43.548 496 151 11 15 421 10 465 7.72e-113 339
MS.gene073653.t1 MTR_1g035620 45.278 413 180 11 26 419 23 408 2.00e-105 318
MS.gene073653.t1 MTR_1g050845 43.717 382 155 11 26 387 23 364 9.29e-83 271
MS.gene073653.t1 MTR_6g061070 33.991 456 241 15 17 420 23 470 1.78e-66 219
MS.gene073653.t1 MTR_1g035650 43.902 205 71 3 26 210 23 203 1.34e-44 154
MS.gene073653.t1 MTR_8g103940 26.087 460 255 17 26 419 53 493 3.53e-37 142
MS.gene073653.t1 MTR_1g108780 26.797 459 256 15 27 419 12 456 1.01e-34 134
MS.gene073653.t1 MTR_5g044670 31.359 287 166 7 28 294 32 307 2.51e-34 130
MS.gene073653.t1 MTR_7g050710 30.820 305 167 10 26 300 27 317 3.87e-33 127
MS.gene073653.t1 MTR_3g098790 32.727 275 173 8 26 290 27 299 1.00e-31 123
MS.gene073653.t1 MTR_7g050560 32.721 272 168 8 26 290 27 290 1.18e-31 123
MS.gene073653.t1 MTR_7g050590 32.982 285 163 9 28 290 2 280 1.01e-29 117
MS.gene073653.t1 MTR_7g050580 34.182 275 160 10 26 290 27 290 4.39e-28 113
MS.gene073653.t1 MTR_1g035480 39.241 158 73 4 17 170 22 160 6.48e-27 106
MS.gene073653.t1 MTR_3g061310 27.465 284 178 9 18 288 16 284 1.56e-26 108
MS.gene073653.t1 MTR_1g035510 32.883 222 124 8 28 233 2 214 1.74e-26 106
MS.gene073653.t1 MTR_7g050720 31.615 291 170 11 28 290 26 315 5.09e-26 107
MS.gene073653.t1 MTR_1g033740 35.484 155 85 3 17 170 27 167 7.27e-26 104
MS.gene073653.t1 MTR_3g061350 25.088 283 197 6 12 288 10 283 2.71e-24 102
MS.gene073653.t1 MTR_1g035460 24.367 316 181 8 28 301 2 301 9.68e-24 102
MS.gene073653.t1 MTR_3g061340 27.174 276 178 11 18 288 16 273 1.77e-23 99.8
MS.gene073653.t1 MTR_6g043890 27.820 266 174 8 26 276 29 291 3.57e-23 100
MS.gene073653.t1 MTR_1g035470 35.036 137 75 2 28 164 2 124 2.34e-22 92.8
MS.gene073653.t1 MTR_3g061370 25.795 283 183 8 18 288 13 280 5.99e-22 95.5
MS.gene073653.t1 MTR_3g061320 25.267 281 182 8 18 288 16 278 6.32e-22 95.5
MS.gene073653.t1 MTR_1g035500 35.256 156 98 2 16 171 29 181 7.43e-22 92.8
MS.gene073653.t1 MTR_4g119990 27.465 284 183 9 26 288 29 310 3.26e-21 95.1
MS.gene073653.t1 MTR_1g035520 25.392 319 193 7 9 288 70 382 9.47e-21 94.0
MS.gene073653.t1 MTR_1g035520 38.053 113 57 4 321 420 271 383 1.61e-12 68.9
MS.gene073653.t1 MTR_3g061290 36.522 115 69 2 18 130 16 128 2.39e-17 80.5
MS.gene073653.t1 MTR_3g061330 35.652 115 70 2 18 130 16 128 2.82e-17 81.6
MS.gene073653.t1 MTR_7g050640 40.449 89 49 2 26 110 27 115 1.28e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 80 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAAAGCCTTTCAAATA+TGG 0.180110 1.1:+35271105 MS.gene073653:CDS
CCATGACAAGGTTATAAAAT+AGG 0.185443 1.1:+35272295 MS.gene073653:intron
AAATATCTTGGAGTTGAAAA+TGG 0.201158 1.1:+35272989 MS.gene073653:CDS
TTAGCTGTGCTTCTTTATTA+AGG 0.220445 1.1:+35273173 MS.gene073653:CDS
TATTTCTCCACATATTTCTT+TGG 0.248757 1.1:-35270970 None:intergenic
CATGACAAGGTTATAAAATA+GGG 0.250371 1.1:+35272296 MS.gene073653:intron
CATTAACATCTTCAGATCTT+AGG 0.260861 1.1:-35271775 None:intergenic
TTCATCAACTGATTTGATAT+TGG 0.261684 1.1:-35271211 None:intergenic
TTAATGGAACTGCAACAATA+AGG 0.277244 1.1:+35271968 MS.gene073653:CDS
AGAAATATGTGGAGAAATAT+TGG 0.281812 1.1:+35270974 MS.gene073653:CDS
TGGAGAGTAATGGTGATATT+TGG 0.287546 1.1:+35271061 MS.gene073653:CDS
GCATAACACATTCAAGGTTT+TGG 0.294441 1.1:+35272831 MS.gene073653:CDS
CTCTTAACTTTCAAATTCAT+TGG 0.314459 1.1:+35271132 MS.gene073653:CDS
TTAGGAAACTTAAGGAATAT+AGG 0.318222 1.1:-35271012 None:intergenic
ATAGTAACATTGAAAGAAAT+TGG 0.322432 1.1:-35272138 None:intergenic
AGAAAACCTAGTGATTTCTT+TGG 0.323706 1.1:-35272186 None:intergenic
AGAGCAATACCCTTTACTTT+TGG 0.331691 1.1:-35272379 None:intergenic
CCTAGTGATTTCTTTGGCTT+TGG 0.332979 1.1:-35272180 None:intergenic
CTGGTCCTTTGAGTTTGCTT+TGG 0.341746 1.1:-35271351 None:intergenic
TAGGAAACTTAAGGAATATA+GGG 0.346499 1.1:-35271011 None:intergenic
AATATCTTGGAGTTGAAAAT+GGG 0.359463 1.1:+35272990 MS.gene073653:CDS
GTGTTTGATGTTTCAATTGT+TGG 0.364550 1.1:+35273140 MS.gene073653:CDS
GTGATGATACACACACAAAT+AGG 0.369595 1.1:+35270265 MS.gene073653:CDS
TAATGGAACTGCAACAATAA+GGG 0.376410 1.1:+35271969 MS.gene073653:CDS
TAATAAAGAAGCACAGCTAA+TGG 0.396679 1.1:-35273170 None:intergenic
CAACACCGTTAGGAAACTTA+AGG 0.403140 1.1:-35271020 None:intergenic
ATAGTTTATTTCTAAAGTAT+TGG 0.406626 1.1:-35271187 None:intergenic
GGGATATAATGAAAATATCT+TGG 0.418536 1.1:+35272977 MS.gene073653:CDS
GTGGTGGATGGAAAATATTC+AGG 0.420444 1.1:+35272058 MS.gene073653:CDS
AAAGAATGCAAAGTGGAGAT+TGG 0.426567 1.1:+35273077 MS.gene073653:CDS
AGCTACAAAGGAACCATGCC+TGG 0.433161 1.1:+35269641 None:intergenic
ACCAAAGGAGTTTATGAAAA+GGG 0.442898 1.1:+35272956 MS.gene073653:CDS
CAAACTCAAAGGACCAGAAA+TGG 0.451853 1.1:+35271357 MS.gene073653:CDS
GATGAAGCATAACACATTCA+AGG 0.455053 1.1:+35272825 MS.gene073653:CDS
GTGTTATGCTTCATCACATC+AGG 0.468230 1.1:-35272817 None:intergenic
AAGAGCCATCCAAAAGTAAA+GGG 0.469681 1.1:+35272370 MS.gene073653:CDS
TACCAAAGGAGTTTATGAAA+AGG 0.473211 1.1:+35272955 MS.gene073653:CDS
TTAACTTTCAAATTCATTGG+TGG 0.475675 1.1:+35271135 MS.gene073653:CDS
TAAGAGCCATCCAAAAGTAA+AGG 0.478853 1.1:+35272369 MS.gene073653:CDS
CAAAGTGGCCATGGTAAAGA+AGG 0.488314 1.1:+35271742 MS.gene073653:CDS
TGACATTATAACTTCAAAAG+AGG 0.495408 1.1:-35271804 None:intergenic
CCATGCCTGGAGCAGTTTCA+AGG 0.511461 1.1:+35269654 None:intergenic
AAATACCTTGAAACTGCTCC+AGG 0.519839 1.1:-35269659 None:intergenic
AAGAGATATCCATATTTGAA+AGG 0.520085 1.1:-35271114 None:intergenic
AATGGCAAGAGAAAATTCTC+AGG 0.523324 1.1:+35271375 MS.gene073653:CDS
GACATTATAACTTCAAAAGA+GGG 0.525934 1.1:-35271803 None:intergenic
AATATCAAATCAGTTGATGA+AGG 0.540855 1.1:+35271213 MS.gene073653:CDS
CAATAAGGGTTGGTAATGAA+AGG 0.551596 1.1:+35271983 MS.gene073653:CDS
CCAAAGCCAAAGAAATCACT+AGG 0.556666 1.1:+35272180 MS.gene073653:CDS
GTAAATTTGAGATGATTCGA+CGG 0.557247 1.1:+35272112 MS.gene073653:CDS
GTTGTAAAGAAGCAAGACAT+TGG 0.558494 1.1:+35272337 MS.gene073653:CDS
TGTTCTCTTGAAAACTCACT+TGG 0.561927 1.1:-35271934 None:intergenic
TTTGGTGAAGAACAATGCCC+CGG 0.564050 1.1:+35272849 MS.gene073653:CDS
GTATTTGCAGAGTGTACCAA+AGG 0.570521 1.1:+35272941 MS.gene073653:intron
AGTGGCTGCAAGTTTAGTAA+AGG 0.571811 1.1:+35273038 MS.gene073653:CDS
AGGTAGTAACAAAGTGGCCA+TGG 0.577811 1.1:+35271733 MS.gene073653:intron
CCTTGAAACTGCTCCAGGCA+TGG 0.579128 1.1:-35269654 None:intergenic
GGAACTGCAACAATAAGGGT+TGG 0.581562 1.1:+35271973 MS.gene073653:CDS
GCTGCAAGTTTAGTAAAGGA+TGG 0.582172 1.1:+35273042 MS.gene073653:CDS
CCAAAGGAGTTTATGAAAAG+GGG 0.584375 1.1:+35272957 MS.gene073653:CDS
AATATTGTATGGGTGGTGGA+TGG 0.587908 1.1:+35272046 MS.gene073653:CDS
GGTGCTGAAGACACAGAAGA+AGG 0.588176 1.1:+35271234 MS.gene073653:CDS
ATATTCCTTAAGTTTCCTAA+CGG 0.590322 1.1:+35271015 MS.gene073653:CDS
GAGTAATGAGAGTTCAGATG+AGG 0.591415 1.1:+35271320 MS.gene073653:CDS
GAGGGTTCCAAAGAAATATG+TGG 0.594786 1.1:+35270963 MS.gene073653:intron
AAAGAAAGAGATGAATCAAG+TGG 0.595556 1.1:+35271258 MS.gene073653:CDS
ATTTATGAAAGAATGCAAAG+TGG 0.597056 1.1:+35273070 MS.gene073653:CDS
ATGACAAGGTTATAAAATAG+GGG 0.601908 1.1:+35272297 MS.gene073653:intron
GTAAAGAAGCAAGACATTGG+AGG 0.602206 1.1:+35272340 MS.gene073653:CDS
ACTTACAAAGAAATAGCCAT+TGG 0.605595 1.1:-35271837 None:intergenic
ATGTCACAATCTTACGCCAA+TGG 0.605717 1.1:+35271821 MS.gene073653:CDS
GGTTGGTAATGAAAGGAGTG+TGG 0.605745 1.1:+35271990 MS.gene073653:CDS
TATGTGGAGAAATATTGGAA+AGG 0.617787 1.1:+35270979 MS.gene073653:CDS
TGAAAATGGGGAGAAAGTCA+TGG 0.631530 1.1:+35273003 MS.gene073653:CDS
TATAAAATAGGGGTCTCTTG+TGG 0.639038 1.1:+35272307 MS.gene073653:CDS
ATATCTTGGAGTTGAAAATG+GGG 0.639227 1.1:+35272991 MS.gene073653:CDS
TCATGGTTCGTAAAAGTCAG+TGG 0.652056 1.1:+35273020 MS.gene073653:CDS
AAATACCAAAGCAAACTCAA+AGG 0.654830 1.1:+35271346 MS.gene073653:CDS
GGAGTGTGGAAGTGAGTGTG+AGG 0.659449 1.1:+35272004 MS.gene073653:CDS
CTTGGCAGGTAGTAACAAAG+TGG 0.671173 1.1:+35271727 MS.gene073653:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTTAATATTTAGTAAT+TGG + chr1.1:35272616-35272635 MS.gene073653:intron 10.0%
!!! TATAAAAATGTGATTTTTTT+TGG + chr1.1:35271585-35271604 MS.gene073653:intron 10.0%
!! AAACTTATGAATGATTTATA+AGG + chr1.1:35272507-35272526 MS.gene073653:intron 15.0%
!! CTAACATATAATTTATAGTT+AGG - chr1.1:35272683-35272702 None:intergenic 15.0%
!!! ATAGTTTATTTCTAAAGTAT+TGG - chr1.1:35271190-35271209 None:intergenic 15.0%
!!! ATATTTGGTTTCAAAAAAAT+TGG + chr1.1:35271076-35271095 MS.gene073653:CDS 15.0%
!!! ATTTAATCAATTTTGTTAGT+TGG + chr1.1:35270111-35270130 MS.gene073653:intron 15.0%
!!! ATTTTAAGAGTTATTGAATA+TGG + chr1.1:35271484-35271503 MS.gene073653:intron 15.0%
!!! ATTTTCTATCTAATTTTTTC+TGG - chr1.1:35270584-35270603 None:intergenic 15.0%
!!! TAAACATCTCTTAATTTTTT+GGG + chr1.1:35272738-35272757 MS.gene073653:intron 15.0%
!!! TAAAGATATTTTTTGTAGTA+TGG - chr1.1:35270661-35270680 None:intergenic 15.0%
!!! TATTTGGTTTCAAAAAAATT+GGG + chr1.1:35271077-35271096 MS.gene073653:CDS 15.0%
!!! TATTTTAAGGTTAAGATATA+TGG + chr1.1:35271162-35271181 MS.gene073653:CDS 15.0%
!!! TGTTGAACAAAAAATATTTT+GGG + chr1.1:35271038-35271057 MS.gene073653:CDS 15.0%
!!! TTAAACATCTCTTAATTTTT+TGG + chr1.1:35272737-35272756 MS.gene073653:intron 15.0%
!!! TTAGATTTTATTATTGTAGA+GGG + chr1.1:35272417-35272436 MS.gene073653:intron 15.0%
!!! TTTAGATTTTATTATTGTAG+AGG + chr1.1:35272416-35272435 MS.gene073653:intron 15.0%
!!! TTTTGGGTATTATAAAAATT+TGG + chr1.1:35272754-35272773 MS.gene073653:intron 15.0%
!! AAATATGATTTCAAATTCAC+AGG - chr1.1:35272583-35272602 None:intergenic 20.0%
!! AACCAAGTACTTATATATTA+TGG - chr1.1:35269819-35269838 None:intergenic 20.0%
!! AAGATTATTCTATGAAAATC+TGG - chr1.1:35270809-35270828 None:intergenic 20.0%
!! ATAGTAACATTGAAAGAAAT+TGG - chr1.1:35272141-35272160 None:intergenic 20.0%
!! ATTATGCATGTATATATTAC+AGG + chr1.1:35269880-35269899 MS.gene073653:intron 20.0%
!! TTATGCATGTATATATTACA+GGG + chr1.1:35269881-35269900 MS.gene073653:intron 20.0%
!!! AAAATGTGATTTTTTTTGGT+AGG + chr1.1:35271589-35271608 MS.gene073653:intron 20.0%
!!! GTGTTGAACAAAAAATATTT+TGG + chr1.1:35271037-35271056 MS.gene073653:CDS 20.0%
!!! TCTTTCGTTATTATTGTTTT+TGG + chr1.1:35269681-35269700 MS.gene073653:intron 20.0%
! AAATAGTTAATGCCTAAACT+TGG + chr1.1:35271709-35271728 MS.gene073653:intron 25.0%
! AAATATCTTGGAGTTGAAAA+TGG + chr1.1:35272989-35273008 MS.gene073653:CDS 25.0%
! AAGAGATATCCATATTTGAA+AGG - chr1.1:35271117-35271136 None:intergenic 25.0%
! AATATATCACCTTTAGTACT+TGG - chr1.1:35270000-35270019 None:intergenic 25.0%
! AATATCTTGGAGTTGAAAAT+GGG + chr1.1:35272990-35273009 MS.gene073653:CDS 25.0%
! ACCCAGAAAAAATATTGTAT+GGG + chr1.1:35272036-35272055 MS.gene073653:CDS 25.0%
! AGAAATATGTGGAGAAATAT+TGG + chr1.1:35270974-35270993 MS.gene073653:CDS 25.0%
! ATATTCCTTAAGTTTCCTAA+CGG + chr1.1:35271015-35271034 MS.gene073653:CDS 25.0%
! ATGACAAGGTTATAAAATAG+GGG + chr1.1:35272297-35272316 MS.gene073653:intron 25.0%
! ATTTATGAAAGAATGCAAAG+TGG + chr1.1:35273070-35273089 MS.gene073653:CDS 25.0%
! CACCATAATATATAAGTACT+TGG + chr1.1:35269814-35269833 MS.gene073653:intron 25.0%
! CATGACAAGGTTATAAAATA+GGG + chr1.1:35272296-35272315 MS.gene073653:intron 25.0%
! CTCTTAACTTTCAAATTCAT+TGG + chr1.1:35271132-35271151 MS.gene073653:CDS 25.0%
! GAATGAAACATTCAATGATT+CGG + chr1.1:35270218-35270237 MS.gene073653:intron 25.0%
! GACATTATAACTTCAAAAGA+GGG - chr1.1:35271806-35271825 None:intergenic 25.0%
! GGGATATAATGAAAATATCT+TGG + chr1.1:35272977-35272996 MS.gene073653:CDS 25.0%
! GTAAAAATGGCAGATATTAA+GGG + chr1.1:35270382-35270401 MS.gene073653:intron 25.0%
! TACAGTACCTTATGTAAAAA+TGG + chr1.1:35270369-35270388 MS.gene073653:intron 25.0%
! TACCCAGAAAAAATATTGTA+TGG + chr1.1:35272035-35272054 MS.gene073653:CDS 25.0%
! TAGGAAACTTAAGGAATATA+GGG - chr1.1:35271014-35271033 None:intergenic 25.0%
! TATTTCTCCACATATTTCTT+TGG - chr1.1:35270973-35270992 None:intergenic 25.0%
! TGAATGATTTATAAGGTTCT+TGG + chr1.1:35272514-35272533 MS.gene073653:intron 25.0%
! TGACATTATAACTTCAAAAG+AGG - chr1.1:35271807-35271826 None:intergenic 25.0%
! TGTAAAAATGGCAGATATTA+AGG + chr1.1:35270381-35270400 MS.gene073653:intron 25.0%
! TTAACTTTCAAATTCATTGG+TGG + chr1.1:35271135-35271154 MS.gene073653:CDS 25.0%
! TTAGGAAACTTAAGGAATAT+AGG - chr1.1:35271015-35271034 None:intergenic 25.0%
!! ACTTTATGATGACATAGTTT+TGG - chr1.1:35270161-35270180 None:intergenic 25.0%
!! AGTTATTGAATATGGTTTGT+TGG + chr1.1:35271492-35271511 MS.gene073653:intron 25.0%
!! GAACACTTGAAAGATTTTAA+TGG + chr1.1:35271952-35271971 MS.gene073653:CDS 25.0%
!! TTTTATGTAACTAATCCTAC+CGG - chr1.1:35272871-35272890 None:intergenic 25.0%
!!! AATATCAAATCAGTTGATGA+AGG + chr1.1:35271213-35271232 MS.gene073653:CDS 25.0%
!!! ACCCATACAATATTTTTTCT+GGG - chr1.1:35272040-35272059 None:intergenic 25.0%
!!! AGTTTTTTTCCAAGTACTAA+AGG + chr1.1:35269988-35270007 MS.gene073653:intron 25.0%
!!! ATATCTGCCATTTTTACATA+AGG - chr1.1:35270379-35270398 None:intergenic 25.0%
!!! ATCTTTTTTGGGTAGATTTA+GGG + chr1.1:35271406-35271425 MS.gene073653:intron 25.0%
!!! ATTTTTACATAAGGTACTGT+AGG - chr1.1:35270370-35270389 None:intergenic 25.0%
!!! CTTTTAATTTACCACAAAAG+TGG - chr1.1:35270302-35270321 None:intergenic 25.0%
!!! CTTTTTTATCGTGTCATAAA+TGG + chr1.1:35270528-35270547 MS.gene073653:intron 25.0%
!!! GGTTTTAGTCATTCTAATAT+TGG + chr1.1:35269835-35269854 MS.gene073653:intron 25.0%
!!! GTTAGGTTTTAAACTAGAAA+AGG - chr1.1:35272666-35272685 None:intergenic 25.0%
!!! TGAACAAAAAATATTTTGGG+TGG + chr1.1:35271041-35271060 MS.gene073653:CDS 25.0%
!!! TTCATCAACTGATTTGATAT+TGG - chr1.1:35271214-35271233 None:intergenic 25.0%
!!! TTTTTACATAAGGTACTGTA+GGG - chr1.1:35270369-35270388 None:intergenic 25.0%
!!! TTTTTTTGTTATCCATGACA+AGG + chr1.1:35272283-35272302 MS.gene073653:intron 25.0%
AAAAAATATTGTATGGGTGG+TGG + chr1.1:35272042-35272061 MS.gene073653:CDS 30.0%
AAAGAAAGAGATGAATCAAG+TGG + chr1.1:35271258-35271277 MS.gene073653:CDS 30.0%
AAATACCAAAGCAAACTCAA+AGG + chr1.1:35271346-35271365 MS.gene073653:CDS 30.0%
ACCAAAGGAGTTTATGAAAA+GGG + chr1.1:35272956-35272975 MS.gene073653:CDS 30.0%
ACTTACAAAGAAATAGCCAT+TGG - chr1.1:35271840-35271859 None:intergenic 30.0%
AGAAAACCTAGTGATTTCTT+TGG - chr1.1:35272189-35272208 None:intergenic 30.0%
ATATCTTGGAGTTGAAAATG+GGG + chr1.1:35272991-35273010 MS.gene073653:CDS 30.0%
CAGAAAAAATATTGTATGGG+TGG + chr1.1:35272039-35272058 MS.gene073653:CDS 30.0%
CATATATGTTCATCTTGGAA+TGG + chr1.1:35269790-35269809 MS.gene073653:intron 30.0%
CATTAACATCTTCAGATCTT+AGG - chr1.1:35271778-35271797 None:intergenic 30.0%
CATTCCAAGATGAACATATA+TGG - chr1.1:35269792-35269811 None:intergenic 30.0%
CCATGACAAGGTTATAAAAT+AGG + chr1.1:35272295-35272314 MS.gene073653:intron 30.0%
CTAAAGGTGATATATTTGTC+AGG + chr1.1:35270004-35270023 MS.gene073653:intron 30.0%
GCATACATTCAAAAACACAT+TGG - chr1.1:35270442-35270461 None:intergenic 30.0%
GTGTTTGATGTTTCAATTGT+TGG + chr1.1:35273140-35273159 MS.gene073653:CDS 30.0%
TAAACCATTACTTGAAACAG+TGG + chr1.1:35271908-35271927 MS.gene073653:intron 30.0%
TAATGGAACTGCAACAATAA+GGG + chr1.1:35271969-35271988 MS.gene073653:CDS 30.0%
TACCAAAGGAGTTTATGAAA+AGG + chr1.1:35272955-35272974 MS.gene073653:CDS 30.0%
TATGTGGAGAAATATTGGAA+AGG + chr1.1:35270979-35270998 MS.gene073653:CDS 30.0%
TCAACCATATATGTTCATCT+TGG + chr1.1:35269785-35269804 MS.gene073653:intron 30.0%
TTAATGGAACTGCAACAATA+AGG + chr1.1:35271968-35271987 MS.gene073653:CDS 30.0%
TTGAATTTCTTTGTTGCAGA+GGG + chr1.1:35270945-35270964 MS.gene073653:intron 30.0%
TTTATGTAACTAATCCTACC+GGG - chr1.1:35272870-35272889 None:intergenic 30.0%
TTTGAATTTCTTTGTTGCAG+AGG + chr1.1:35270944-35270963 MS.gene073653:intron 30.0%
TTTGCAAAGCCTTTCAAATA+TGG + chr1.1:35271105-35271124 MS.gene073653:CDS 30.0%
! CCTATTTTATAACCTTGTCA+TGG - chr1.1:35272298-35272317 None:intergenic 30.0%
! GTAAATTTGAGATGATTCGA+CGG + chr1.1:35272112-35272131 MS.gene073653:CDS 30.0%
! GTTGGAATTTTATGTCTGAA+AGG + chr1.1:35271510-35271529 MS.gene073653:intron 30.0%
! TAATAAAGAAGCACAGCTAA+TGG - chr1.1:35273173-35273192 None:intergenic 30.0%
!! CATTGGTGGATCATATTTTA+AGG + chr1.1:35271149-35271168 MS.gene073653:CDS 30.0%
!!! AGGTGAGTTTGATCTTTTTT+GGG + chr1.1:35271395-35271414 MS.gene073653:intron 30.0%
!!! ATTTTTTGTTCAACACCGTT+AGG - chr1.1:35271033-35271052 None:intergenic 30.0%
!!! CACCCATACAATATTTTTTC+TGG - chr1.1:35272041-35272060 None:intergenic 30.0%
!!! GATCTTTTTTGGGTAGATTT+AGG + chr1.1:35271405-35271424 MS.gene073653:intron 30.0%
!!! TTTGGCTCAAATCACTTTTT+AGG - chr1.1:35270143-35270162 None:intergenic 30.0%
!!! TTTTTCTGGTGTTACTACTT+TGG - chr1.1:35270570-35270589 None:intergenic 30.0%
AAAGAATGCAAAGTGGAGAT+TGG + chr1.1:35273077-35273096 MS.gene073653:CDS 35.0%
AACTGAATGCTGGATAAGTT+AGG - chr1.1:35272642-35272661 None:intergenic 35.0%
AAGAGCCATCCAAAAGTAAA+GGG + chr1.1:35272370-35272389 MS.gene073653:CDS 35.0%
AATGGCAAGAGAAAATTCTC+AGG + chr1.1:35271375-35271394 MS.gene073653:CDS 35.0%
ACTGGACAGTAATAAAGTCT+TGG + chr1.1:35270628-35270647 MS.gene073653:intron 35.0%
ACTTTATTACTGTCCAGTAC+AGG - chr1.1:35270626-35270645 None:intergenic 35.0%
CCAAAGGAGTTTATGAAAAG+GGG + chr1.1:35272957-35272976 MS.gene073653:CDS 35.0%
GATGAAGCATAACACATTCA+AGG + chr1.1:35272825-35272844 MS.gene073653:CDS 35.0%
GTGATGATACACACACAAAT+AGG + chr1.1:35270265-35270284 MS.gene073653:CDS 35.0%
GTTGTAAAGAAGCAAGACAT+TGG + chr1.1:35272337-35272356 MS.gene073653:CDS 35.0%
TAAGAGCCATCCAAAAGTAA+AGG + chr1.1:35272369-35272388 MS.gene073653:CDS 35.0%
TAGAAAAGGAAACTGAATGC+TGG - chr1.1:35272652-35272671 None:intergenic 35.0%
TATAAAATAGGGGTCTCTTG+TGG + chr1.1:35272307-35272326 MS.gene073653:CDS 35.0%
TGGAGAGTAATGGTGATATT+TGG + chr1.1:35271061-35271080 MS.gene073653:CDS 35.0%
TGTTCTCTTGAAAACTCACT+TGG - chr1.1:35271937-35271956 None:intergenic 35.0%
TTATGCCTGTATTGTACAGA+AGG + chr1.1:35272793-35272812 MS.gene073653:intron 35.0%
TTATGTAACTAATCCTACCG+GGG - chr1.1:35272869-35272888 None:intergenic 35.0%
! AGAGCAATACCCTTTACTTT+TGG - chr1.1:35272382-35272401 None:intergenic 35.0%
! GCATAACACATTCAAGGTTT+TGG + chr1.1:35272831-35272850 MS.gene073653:CDS 35.0%
! GTATCCACTGTTTCAAGTAA+TGG - chr1.1:35271915-35271934 None:intergenic 35.0%
!! ACAAAGCAAATCCACTTTTG+TGG + chr1.1:35270288-35270307 MS.gene073653:CDS 35.0%
!! ATATTTTGGGTGGAGAGTAA+TGG + chr1.1:35271051-35271070 MS.gene073653:CDS 35.0%
!! CAATAAGGGTTGGTAATGAA+AGG + chr1.1:35271983-35272002 MS.gene073653:CDS 35.0%
!! CAATACCCTTTACTTTTGGA+TGG - chr1.1:35272378-35272397 None:intergenic 35.0%
!! GAATTTTCTCTTGCCATTTC+TGG - chr1.1:35271373-35271392 None:intergenic 35.0%
!! GGTGAAAGATTTTGAGTGTT+AGG - chr1.1:35270726-35270745 None:intergenic 35.0%
!! TTTTCTAGCCTTCTTTACCA+TGG - chr1.1:35271753-35271772 None:intergenic 35.0%
!!! CAGGTGAGTTTGATCTTTTT+TGG + chr1.1:35271394-35271413 MS.gene073653:intron 35.0%
AAATACCTTGAAACTGCTCC+AGG - chr1.1:35269662-35269681 None:intergenic 40.0%
AGTGGCTGCAAGTTTAGTAA+AGG + chr1.1:35273038-35273057 MS.gene073653:CDS 40.0%
AGTTAATGCCTAAACTTGGC+AGG + chr1.1:35271713-35271732 MS.gene073653:intron 40.0%
ATGTCACAATCTTACGCCAA+TGG + chr1.1:35271821-35271840 MS.gene073653:CDS 40.0%
CAAACTCAAAGGACCAGAAA+TGG + chr1.1:35271357-35271376 MS.gene073653:CDS 40.0%
CAACACCGTTAGGAAACTTA+AGG - chr1.1:35271023-35271042 None:intergenic 40.0%
CATTGTGAGCTCATGTGAAA+TGG + chr1.1:35270342-35270361 MS.gene073653:intron 40.0%
CCAAAGCCAAAGAAATCACT+AGG + chr1.1:35272180-35272199 MS.gene073653:CDS 40.0%
GAGGGTTCCAAAGAAATATG+TGG + chr1.1:35270963-35270982 MS.gene073653:intron 40.0%
GAGTAATGAGAGTTCAGATG+AGG + chr1.1:35271320-35271339 MS.gene073653:CDS 40.0%
GCTGCAAGTTTAGTAAAGGA+TGG + chr1.1:35273042-35273061 MS.gene073653:CDS 40.0%
GGATCAAAGGAAAAGACACA+CGG + chr1.1:35270403-35270422 MS.gene073653:intron 40.0%
GGCAGATATTAAGGGATCAA+AGG + chr1.1:35270390-35270409 MS.gene073653:intron 40.0%
GTAAAGAAGCAAGACATTGG+AGG + chr1.1:35272340-35272359 MS.gene073653:CDS 40.0%
GTATTTGCAGAGTGTACCAA+AGG + chr1.1:35272941-35272960 MS.gene073653:intron 40.0%
GTGTTATGCTTCATCACATC+AGG - chr1.1:35272820-35272839 None:intergenic 40.0%
TCATGGTTCGTAAAAGTCAG+TGG + chr1.1:35273020-35273039 MS.gene073653:CDS 40.0%
TGAAAATGGGGAGAAAGTCA+TGG + chr1.1:35273003-35273022 MS.gene073653:CDS 40.0%
TGTTACTACCTGCCAAGTTT+AGG - chr1.1:35271724-35271743 None:intergenic 40.0%
TTTCTCTACATGACCTGTAC+TGG + chr1.1:35270610-35270629 MS.gene073653:intron 40.0%
! CCCCTTTTCATAAACTCCTT+TGG - chr1.1:35272960-35272979 None:intergenic 40.0%
! CCTAGTGATTTCTTTGGCTT+TGG - chr1.1:35272183-35272202 None:intergenic 40.0%
!! AATATTGTATGGGTGGTGGA+TGG + chr1.1:35272046-35272065 MS.gene073653:CDS 40.0%
!! AGTGAGTGAGTATTGAGCTA+AGG - chr1.1:35270762-35270781 None:intergenic 40.0%
!! CAAGCACTGAAACAGAACAA+TGG + chr1.1:35269930-35269949 MS.gene073653:intron 40.0%
!! GTGGTGGATGGAAAATATTC+AGG + chr1.1:35272058-35272077 MS.gene073653:CDS 40.0%
!! TGGTGTTACTACTTTGGTGT+TGG - chr1.1:35270564-35270583 None:intergenic 40.0%
!!! CTTTGGCTTTGGTTCGTTTT+TGG - chr1.1:35272172-35272191 None:intergenic 40.0%
AGCTAAGGCTTAGAGAAGTG+AGG - chr1.1:35270747-35270766 None:intergenic 45.0%
AGGTAGTAACAAAGTGGCCA+TGG + chr1.1:35271733-35271752 MS.gene073653:intron 45.0%
CAAAGTGGCCATGGTAAAGA+AGG + chr1.1:35271742-35271761 MS.gene073653:CDS 45.0%
CAGGACCTTCTGTACAATAC+AGG - chr1.1:35272801-35272820 None:intergenic 45.0%
CTTGGCAGGTAGTAACAAAG+TGG + chr1.1:35271727-35271746 MS.gene073653:intron 45.0%
GGAACTGCAACAATAAGGGT+TGG + chr1.1:35271973-35271992 MS.gene073653:CDS 45.0%
TGTATGCAGTGTGAGACTGT+TGG + chr1.1:35270455-35270474 MS.gene073653:intron 45.0%
TTTGGTGAAGAACAATGCCC+CGG + chr1.1:35272849-35272868 MS.gene073653:CDS 45.0%
! CTGGTCCTTTGAGTTTGCTT+TGG - chr1.1:35271354-35271373 None:intergenic 45.0%
!! GGTTGGTAATGAAAGGAGTG+TGG + chr1.1:35271990-35272009 MS.gene073653:CDS 45.0%
!! AAAAATAATAAATACTTAAT+TGG + chr1.1:35271640-35271659 MS.gene073653:intron 5.0%
!! ATTATATATATTTCTTAATT+TGG + chr1.1:35272479-35272498 MS.gene073653:intron 5.0%
! GGTGCTGAAGACACAGAAGA+AGG + chr1.1:35271234-35271253 MS.gene073653:CDS 50.0%
GGAGTGTGGAAGTGAGTGTG+AGG + chr1.1:35272004-35272023 MS.gene073653:CDS 55.0%
GTGAAGAACAATGCCCCGGT+AGG + chr1.1:35272853-35272872 MS.gene073653:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 35269656 35273193 35269656 ID=MS.gene073653
chr1.1 mRNA 35269656 35273193 35269656 ID=MS.gene073653.t1;Parent=MS.gene073653
chr1.1 exon 35269656 35269675 35269656 ID=MS.gene073653.t1.exon1;Parent=MS.gene073653.t1
chr1.1 CDS 35269656 35269675 35269656 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35270252 35270309 35270252 ID=MS.gene073653.t1.exon2;Parent=MS.gene073653.t1
chr1.1 CDS 35270252 35270309 35270252 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35270964 35271396 35270964 ID=MS.gene073653.t1.exon3;Parent=MS.gene073653.t1
chr1.1 CDS 35270964 35271396 35270964 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35271735 35271853 35271735 ID=MS.gene073653.t1.exon4;Parent=MS.gene073653.t1
chr1.1 CDS 35271735 35271853 35271735 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35271928 35272201 35271928 ID=MS.gene073653.t1.exon5;Parent=MS.gene073653.t1
chr1.1 CDS 35271928 35272201 35271928 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35272305 35272391 35272305 ID=MS.gene073653.t1.exon6;Parent=MS.gene073653.t1
chr1.1 CDS 35272305 35272391 35272305 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35272812 35272870 35272812 ID=MS.gene073653.t1.exon7;Parent=MS.gene073653.t1
chr1.1 CDS 35272812 35272870 35272812 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
chr1.1 exon 35272951 35273193 35272951 ID=MS.gene073653.t1.exon8;Parent=MS.gene073653.t1
chr1.1 CDS 35272951 35273193 35272951 ID=cds.MS.gene073653.t1;Parent=MS.gene073653.t1
Gene Sequence

>MS.gene073653

ATGCCTGGAGCAGTTTCAAGGTATTTCTTTCGTTATTATTGTTTTTGGAAAGATACATATTTTATTGTAGTTTAATCTTGATACATTTGATCAATGTAAAAAGCTTTTCAATTTGTCAACAAATTACAATCAACCATATATGTTCATCTTGGAATGGTCACCATAATATATAAGTACTTGGTTTTAGTCATTCTAATATTGGCTACATAAAGACTAAAATAAATATTATGCATGTATATATTACAGGGATTAATTCTAACATATACAAATTTTACAAGCACTGAAACAGAACAATGGTCAAAAGTAAAAAATGATATGTTCACTATGATTGAAGTTTTTTTCCAAGTACTAAAGGTGATATATTTGTCAGGACAAAAAACAAATTTAAACTAATTTTTAATGATCTAAGATCATTGATTTATACTGTAAATATCTTTACACATTCATATGAGTTAATTTAATCAATTTTGTTAGTTGGTTTTTACCTAAAAAGTGATTTGAGCCAAAACTATGTCATCATAAAGTAACTAAAGCTATCTAATTAATTAGACATTAGAAAACAGAATGAAACATTCAATGATTCGGTCATGTTAAAGCATGACAGATACTGTGATGATACACACACAAATAGGACAAAGCAAATCCACTTTTGTGGTAAATTAAAAGTTGCTTCTATTTATAGCTTACATTGTGAGCTCATGTGAAATGGACCCTACAGTACCTTATGTAAAAATGGCAGATATTAAGGGATCAAAGGAAAAGACACACGGCTTTGTAATCTAACCAATGTGTTTTTGAATGTATGCAGTGTGAGACTGTTGGATCATCTCAAGCATGTATAGCTTTCACTGAATTGTACTAACTGCTAAACACTTTTTTATCGTGTCATAAATGGCAAGTTCAATCCAACACCAAAGTAGTAACACCAGAAAAAATTAGATAGAAAATTCATCATTTCTCTACATGACCTGTACTGGACAGTAATAAAGTCTTGGTTTTCTACCATACTACAAAAAATATCTTTAACTTCATAACATTCACACTTCACATGATTTATAACATTCAGACCTAACACTCAAAATCTTTCACCTCACTTCTCTAAGCCTTAGCTCAATACTCACTCACTAACATCATGTCATTTCAACATATTCCAGATTTTCATAGAATAATCTTGCAAGATAAGAAACTTGTAAGCATATTAGAAAGAGTTATCTTGCATATTTACATTATGTTTCATTTATAATATATTATTTATGTATCATTTTTTTAAAAATGTTTATGTATGATTTTTGAATTTCTTTGTTGCAGAGGGTTCCAAAGAAATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAGTTTCCTAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAATTTTGCAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATATGGTGCCAATACTTTAGAAATAAACTATTCCAATATCAAATCAGTTGATGAAGGTGCTGAAGACACAGAAGAAGGTAAAGAAAGAGATGAATCAAGTGGTGATGAAGTCACAAAAGAAGCTCAAGAAAGTGATGAAGAGAGTAATGAGAGTTCAGATGAGGTTGAAATACCAAAGCAAACTCAAAGGACCAGAAATGGCAAGAGAAAATTCTCAGGTGAGTTTGATCTTTTTTGGGTAGATTTAGGGTTGTCTGTTACTCTATTTCTTTAAAAATAAATCACTTGTTTTTATAGAAATAATTATTTTAAGAGTTATTGAATATGGTTTGTTGGAATTTTATGTCTGAAAGGTTGAAAATCTTTGAAAGAAAAATGTCTTGAAGTGCTGAGTAGATACTTTTTATATAAAAATGTGATTTTTTTTGGTAGGAAAAATATGTGATTTTTATTATGATAAAAAAAATAATAAATACTTAATTGGTTTTCTTAAAATCTTCTAAAAACAATATCTAAATCAAATGTATAATAAATAGTTAATGCCTAAACTTGGCAGGTAGTAACAAAGTGGCCATGGTAAAGAAGGCTAGAAAATGCCTAAGATCTGAAGATGTTAATGAGAATCCCTCTTTTGAAGTTATAATGTCACAATCTTACGCCAATGGCTATTTCTTTGTAAGTTTGCTATACAAAGTATCTATTAATTTTAGCTTTAATTATTATATCTAATAAACCATTACTTGAAACAGTGGATACCAAGTGAGTTTTCAAGAGAACACTTGAAAGATTTTAATGGAACTGCAACAATAAGGGTTGGTAATGAAAGGAGTGTGGAAGTGAGTGTGAGGTATTATGATACCCAGAAAAAATATTGTATGGGTGGTGGATGGAAAATATTCAGGCAAAAATATAATTTGCAAGTTGATGATGTTTGTAAATTTGAGATGATTCGACGGAGACCAATTTCTTTCAATGTTACTATTACTCGTGCCAAAAACGAACCAAAGCCAAAGAAATCACTAGGTTTTCTTTCTTTCTTTTTTATTTCGTTTTCTGTATGTTATAATTTTTTTTATTTGATATTTGATTAACATCTAATATTTTTTTTTTTGTTATCCATGACAAGGTTATAAAATAGGGGTCTCTTGTGGTAAGAGTGTTGTAAAGAAGCAAGACATTGGAGGAACATCTAAGAGCCATCCAAAAGTAAAGGGTATTGCTCTAACTTTTTAATGTTTTTAGATTTTATTATTGTAGAGGGTTCTGAAATATTTGTATCTTTTAAATAGTTCTTTGAACTATTATATATATTTCTTAATTTGGTCTTCAAACTTATGAATGATTTATAAGGTTCTTGGACTAAGTTTTTAATTGTATATCGTTAATCTTTAGTTTTAAATACCTGTGAATTTGAAATCATATTTATTTACGTTGTTGACTTTTTAATATTTAGTAATTGGCCTAACTTATCCAGCATTCAGTTTCCTTTTCTAGTTTAAAACCTAACTATAAATTATATGTTAGTTCATAATTGAAATTTTTTAGCTCAAAAATATATTTAAACATCTCTTAATTTTTTGGGTATTATAAAAATTTGGTGATATCATTTTGTGTTTATGCCTGTATTGTACAGAAGGTCCTGATGTGATGAAGCATAACACATTCAAGGTTTTGGTGAAGAACAATGCCCCGGTAGGATTAGTTACATAAAATATGAATATATAGATAGAACTACATAACAAATAAATAAAAAAAATTCTTTGTATTTGCAGAGTGTACCAAAGGAGTTTATGAAAAGGGGATATAATGAAAATATCTTGGAGTTGAAAATGGGGAGAAAGTCATGGTTCGTAAAAGTCAGTGGCTGCAAGTTTAGTAAAGGATGGATAAAATTTATGAAAGAATGCAAAGTGGAGATTGGAGATATTTGTCTCTTCAAGTTAATTGATGAGAGCAAGTTTGTGTTTGATGTTTCAATTGTTGGAAAGAATCCATTAGCTGTGCTTCTTTATTAA
Protein sequence

>MS.gene073653.t1

MPGAVSSMTDTVMIHTQIGQSKSTFVRVPKKYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWENFAKPFKYGYLLTFKFIGGSYFKVKIYGANTLEINYSNIKSVDEGAEDTEEGKERDESSGDEVTKEAQESDEESNESSDEVEIPKQTQRTRNGKRKFSGSNKVAMVKKARKCLRSEDVNENPSFEVIMSQSYANGYFFWIPSEFSREHLKDFNGTATIRVGNERSVEVSVRYYDTQKKYCMGGGWKIFRQKYNLQVDDVCKFEMIRRRPISFNVTITRAKNEPKPKKSLGYKIGVSCGKSVVKKQDIGGTSKSHPKVKEGPDVMKHNTFKVLVKNNAPSVPKEFMKRGYNENILELKMGRKSWFVKVSGCKFSKGWIKFMKECKVEIGDICLFKLIDESKFVFDVSIVGKNPLAVLLY