Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20686.t1 | XP_013469226.1 | 97.3 | 602 | 10 | 2 | 1 | 602 | 1 | 596 | 0.00E+00 | 1161.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20686.t1 | O82261 | 71.2 | 617 | 153 | 7 | 1 | 602 | 1 | 607 | 1.9e-245 | 849.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20686.t1 | A0A072VML9 | 97.3 | 602 | 10 | 2 | 1 | 602 | 1 | 596 | 0.0e+00 | 1161.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene20686 | 0.815598 | 8.52E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene20686 | 0.819417 | 1.17E-52 | -1.69E-46 |
| MS.gene049284 | MS.gene20686 | 0.810967 | 8.86E-51 | -1.69E-46 |
| MS.gene049351 | MS.gene20686 | 0.852527 | 4.36E-61 | -1.69E-46 |
| MS.gene049427 | MS.gene20686 | 0.895401 | 9.93E-76 | -1.69E-46 |
| MS.gene049491 | MS.gene20686 | 0.842024 | 3.33E-58 | -1.69E-46 |
| MS.gene049614 | MS.gene20686 | 0.840861 | 6.74E-58 | -1.69E-46 |
| MS.gene049661 | MS.gene20686 | 0.813592 | 2.37E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene20686 | 0.808766 | 2.64E-50 | -1.69E-46 |
| MS.gene049760 | MS.gene20686 | 0.837092 | 6.37E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene20686 | 0.808729 | 2.68E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene20686 | 0.870456 | 1.44E-66 | -1.69E-46 |
| MS.gene050099 | MS.gene20686 | 0.8364 | 9.57E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene20686 | 0.815893 | 7.32E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene20686 | 0.861082 | 1.32E-63 | -1.69E-46 |
| MS.gene050333 | MS.gene20686 | 0.801778 | 7.67E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene20686 | 0.865556 | 5.42E-65 | -1.69E-46 |
| MS.gene050529 | MS.gene20686 | 0.814564 | 1.44E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene20686 | 0.899132 | 2.68E-77 | -1.69E-46 |
| MS.gene050770 | MS.gene20686 | 0.90228 | 1.14E-78 | -1.69E-46 |
| MS.gene050783 | MS.gene20686 | 0.857778 | 1.29E-62 | -1.69E-46 |
| MS.gene050812 | MS.gene20686 | 0.86785 | 1.01E-65 | -1.69E-46 |
| MS.gene05100 | MS.gene20686 | 0.815617 | 8.44E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene20686 | 0.879492 | 1.19E-69 | -1.69E-46 |
| MS.gene051228 | MS.gene20686 | 0.823986 | 1.03E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene20686 | 0.810247 | 1.27E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene20686 | 0.875343 | 3.32E-68 | -1.69E-46 |
| MS.gene051666 | MS.gene20686 | 0.841452 | 4.71E-58 | -1.69E-46 |
| MS.gene051694 | MS.gene20686 | 0.82319 | 1.58E-53 | -1.69E-46 |
| MS.gene051877 | MS.gene20686 | 0.852652 | 4.01E-61 | -1.69E-46 |
| MS.gene052078 | MS.gene20686 | 0.865471 | 5.76E-65 | -1.69E-46 |
| MS.gene052178 | MS.gene20686 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene20686 | 0.814218 | 1.72E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene20686 | 0.822776 | 1.98E-53 | -1.69E-46 |
| MS.gene052290 | MS.gene20686 | 0.837724 | 4.39E-57 | -1.69E-46 |
| MS.gene052310 | MS.gene20686 | 0.856072 | 4.12E-62 | -1.69E-46 |
| MS.gene052322 | MS.gene20686 | 0.814958 | 1.18E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene20686 | 0.897325 | 1.57E-76 | -1.69E-46 |
| MS.gene052379 | MS.gene20686 | 0.86062 | 1.82E-63 | -1.69E-46 |
| MS.gene052484 | MS.gene20686 | 0.809856 | 1.54E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene20686 | 0.817508 | 3.18E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene20686 | 0.821025 | 5.03E-53 | -1.69E-46 |
| MS.gene053104 | MS.gene20686 | 0.826588 | 2.50E-54 | -1.69E-46 |
| MS.gene053107 | MS.gene20686 | 0.85948 | 4.02E-63 | -1.69E-46 |
| MS.gene053322 | MS.gene20686 | 0.885931 | 5.31E-72 | -1.69E-46 |
| MS.gene054123 | MS.gene20686 | 0.859579 | 3.75E-63 | -1.69E-46 |
| MS.gene054376 | MS.gene20686 | 0.892258 | 1.87E-74 | -1.69E-46 |
| MS.gene05455 | MS.gene20686 | 0.871038 | 9.26E-67 | -1.69E-46 |
| MS.gene054642 | MS.gene20686 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene054802 | MS.gene20686 | 0.913931 | 3.47E-84 | -1.69E-46 |
| MS.gene054803 | MS.gene20686 | 0.91458 | 1.62E-84 | -1.69E-46 |
| MS.gene054804 | MS.gene20686 | 0.914313 | 2.22E-84 | -1.69E-46 |
| MS.gene054955 | MS.gene20686 | 0.833884 | 4.13E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene20686 | 0.849597 | 2.92E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene20686 | 0.86624 | 3.30E-65 | -1.69E-46 |
| MS.gene055390 | MS.gene20686 | 0.877201 | 7.60E-69 | -1.69E-46 |
| MS.gene055397 | MS.gene20686 | 0.881156 | 3.03E-70 | -1.69E-46 |
| MS.gene055412 | MS.gene20686 | 0.83098 | 2.16E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene20686 | 0.851283 | 9.81E-61 | -1.69E-46 |
| MS.gene055428 | MS.gene20686 | 0.843893 | 1.06E-58 | -1.69E-46 |
| MS.gene055499 | MS.gene20686 | 0.877258 | 7.26E-69 | -1.69E-46 |
| MS.gene055636 | MS.gene20686 | 0.812379 | 4.37E-51 | -1.69E-46 |
| MS.gene055638 | MS.gene20686 | 0.810855 | 9.37E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene20686 | 0.818371 | 2.03E-52 | -1.69E-46 |
| MS.gene055896 | MS.gene20686 | 0.874347 | 7.25E-68 | -1.69E-46 |
| MS.gene05592 | MS.gene20686 | 0.810929 | 9.03E-51 | -1.69E-46 |
| MS.gene055930 | MS.gene20686 | 0.83914 | 1.89E-57 | -1.69E-46 |
| MS.gene056407 | MS.gene20686 | 0.820574 | 6.38E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene20686 | 0.848885 | 4.61E-60 | -1.69E-46 |
| MS.gene056452 | MS.gene20686 | 0.859747 | 3.34E-63 | -1.69E-46 |
| MS.gene056609 | MS.gene20686 | 0.829436 | 5.15E-55 | -1.69E-46 |
| MS.gene056612 | MS.gene20686 | 0.824701 | 7.00E-54 | -1.69E-46 |
| MS.gene056796 | MS.gene20686 | 0.836143 | 1.11E-56 | -1.69E-46 |
| MS.gene056897 | MS.gene20686 | 0.801843 | 7.44E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene20686 | 0.874886 | 4.76E-68 | -1.69E-46 |
| MS.gene057123 | MS.gene20686 | 0.834908 | 2.28E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene20686 | 0.885198 | 9.99E-72 | -1.69E-46 |
| MS.gene057146 | MS.gene20686 | 0.86724 | 1.58E-65 | -1.69E-46 |
| MS.gene057295 | MS.gene20686 | 0.855209 | 7.36E-62 | -1.69E-46 |
| MS.gene05733 | MS.gene20686 | 0.80726 | 5.51E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene20686 | 0.809783 | 1.60E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene20686 | 0.834001 | 3.86E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene20686 | 0.835878 | 1.30E-56 | -1.69E-46 |
| MS.gene058082 | MS.gene20686 | 0.813593 | 2.37E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene20686 | 0.833124 | 6.39E-56 | -1.69E-46 |
| MS.gene058233 | MS.gene20686 | 0.820263 | 7.52E-53 | -1.69E-46 |
| MS.gene058293 | MS.gene20686 | 0.89514 | 1.27E-75 | -1.69E-46 |
| MS.gene058323 | MS.gene20686 | 0.83441 | 3.05E-56 | -1.69E-46 |
| MS.gene058365 | MS.gene20686 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene20686 | 0.902848 | 6.37E-79 | -1.69E-46 |
| MS.gene058787 | MS.gene20686 | 0.83014 | 3.47E-55 | -1.69E-46 |
| MS.gene058903 | MS.gene20686 | 0.806164 | 9.39E-50 | -1.69E-46 |
| MS.gene058993 | MS.gene20686 | 0.834513 | 2.87E-56 | -1.69E-46 |
| MS.gene059029 | MS.gene20686 | 0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene20686 | 0.837233 | 5.87E-57 | -1.69E-46 |
| MS.gene059326 | MS.gene20686 | 0.85286 | 3.50E-61 | -1.69E-46 |
| MS.gene059342 | MS.gene20686 | 0.80013 | 1.66E-48 | -1.69E-46 |
| MS.gene05934 | MS.gene20686 | 0.816571 | 5.16E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene20686 | 0.803967 | 2.71E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene20686 | 0.871249 | 7.89E-67 | -1.69E-46 |
| MS.gene059597 | MS.gene20686 | 0.858713 | 6.82E-63 | -1.69E-46 |
| MS.gene05959 | MS.gene20686 | 0.832174 | 1.10E-55 | -1.69E-46 |
| MS.gene059676 | MS.gene20686 | 0.864198 | 1.45E-64 | -1.69E-46 |
| MS.gene059688 | MS.gene20686 | 0.831759 | 1.39E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene20686 | 0.832541 | 8.92E-56 | -1.69E-46 |
| MS.gene059695 | MS.gene20686 | 0.837131 | 6.23E-57 | -1.69E-46 |
| MS.gene059708 | MS.gene20686 | 0.838181 | 3.35E-57 | -1.69E-46 |
| MS.gene059894 | MS.gene20686 | 0.817069 | 3.99E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene20686 | 0.922494 | 9.14E-89 | -1.69E-46 |
| MS.gene060005 | MS.gene20686 | 0.812313 | 4.51E-51 | -1.69E-46 |
| MS.gene060193 | MS.gene20686 | 0.842793 | 2.08E-58 | -1.69E-46 |
| MS.gene06037 | MS.gene20686 | 0.803121 | 4.05E-49 | -1.69E-46 |
| MS.gene060407 | MS.gene20686 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene20686 | 0.83576 | 1.39E-56 | -1.69E-46 |
| MS.gene060818 | MS.gene20686 | 0.853519 | 2.27E-61 | -1.69E-46 |
| MS.gene06090 | MS.gene20686 | 0.864173 | 1.47E-64 | -1.69E-46 |
| MS.gene061041 | MS.gene20686 | 0.828176 | 1.04E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene20686 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene061051 | MS.gene20686 | -0.825846 | 3.75E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene20686 | 0.808304 | 3.31E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene20686 | 0.912362 | 2.12E-83 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60345 | MS.gene20686 | PPI |
| MS.gene033222 | MS.gene20686 | PPI |
| MS.gene43291 | MS.gene20686 | PPI |
| MS.gene56943 | MS.gene20686 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20686.t1 | MTR_1g088980 | 97.342 | 602 | 10 | 2 | 1 | 602 | 1 | 596 | 0.0 | 1200 |
| MS.gene20686.t1 | MTR_4g106730 | 52.381 | 462 | 215 | 2 | 108 | 564 | 127 | 588 | 2.39e-176 | 513 |
| MS.gene20686.t1 | MTR_6g004110 | 47.186 | 462 | 237 | 4 | 108 | 565 | 80 | 538 | 1.90e-139 | 417 |
| MS.gene20686.t1 | MTR_6g004110 | 47.575 | 433 | 220 | 4 | 137 | 565 | 2 | 431 | 2.40e-129 | 387 |
| MS.gene20686.t1 | MTR_6g007030 | 29.537 | 281 | 176 | 9 | 147 | 418 | 161 | 428 | 9.54e-16 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20686.t1 | AT2G47940 | 71.129 | 620 | 148 | 8 | 1 | 602 | 1 | 607 | 0.0 | 875 |
| MS.gene20686.t1 | AT2G47940 | 71.405 | 619 | 147 | 9 | 1 | 602 | 1 | 606 | 0.0 | 873 |
| MS.gene20686.t1 | AT5G40200 | 52.381 | 462 | 215 | 2 | 108 | 564 | 125 | 586 | 5.93e-171 | 499 |
| MS.gene20686.t1 | AT5G36950 | 42.153 | 548 | 289 | 9 | 28 | 565 | 42 | 571 | 9.80e-132 | 398 |
| MS.gene20686.t1 | AT1G65640 | 40.756 | 476 | 264 | 6 | 91 | 563 | 53 | 513 | 1.81e-116 | 357 |
| MS.gene20686.t1 | AT1G65630 | 40.000 | 505 | 286 | 8 | 80 | 580 | 63 | 554 | 1.01e-113 | 351 |
| MS.gene20686.t1 | AT5G40560 | 39.913 | 461 | 252 | 5 | 106 | 565 | 37 | 473 | 2.76e-102 | 319 |
| MS.gene20686.t1 | AT1G65640 | 42.640 | 394 | 213 | 5 | 91 | 481 | 53 | 436 | 1.22e-100 | 313 |
| MS.gene20686.t1 | AT3G16540 | 34.894 | 470 | 266 | 6 | 104 | 569 | 102 | 535 | 1.35e-85 | 278 |
| MS.gene20686.t1 | AT3G16550 | 34.462 | 502 | 284 | 10 | 74 | 563 | 31 | 499 | 3.84e-84 | 272 |
| MS.gene20686.t1 | AT1G51150 | 42.581 | 155 | 86 | 2 | 109 | 262 | 57 | 209 | 1.80e-36 | 136 |
| MS.gene20686.t1 | AT5G54745 | 36.416 | 173 | 82 | 5 | 109 | 278 | 44 | 191 | 2.52e-25 | 104 |
| MS.gene20686.t1 | AT3G27925 | 27.645 | 293 | 189 | 10 | 136 | 418 | 156 | 435 | 9.73e-15 | 77.4 |
| MS.gene20686.t1 | AT3G27925 | 27.645 | 293 | 189 | 10 | 136 | 418 | 156 | 435 | 9.73e-15 | 77.4 |
| MS.gene20686.t1 | AT5G39830 | 27.612 | 268 | 155 | 12 | 166 | 415 | 197 | 443 | 2.91e-12 | 69.7 |
Find 133 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCATTGGGGTGGCATTTC+AGG | 0.121430 | 1.2:-66856074 | MS.gene20686:intron |
| ATTTACCTGTCCTTTGAAAT+TGG | 0.159675 | 1.2:+66860893 | None:intergenic |
| TCATGATCAGCTAAAGTTAT+TGG | 0.185967 | 1.2:-66853182 | MS.gene20686:intron |
| TATTCATTTGCAAGGTTTAA+AGG | 0.216596 | 1.2:-66853148 | MS.gene20686:CDS |
| CAGTAGAAAGCGAGGAGTTT+TGG | 0.221279 | 1.2:-66857375 | MS.gene20686:CDS |
| CCAACTTCAAAATTTGAAAC+TGG | 0.272180 | 1.2:+66852452 | None:intergenic |
| TGATGCAAATAATGTATTAA+AGG | 0.283856 | 1.2:-66854356 | MS.gene20686:CDS |
| CAACCACCAGACCAGGAATT+TGG | 0.295454 | 1.2:-66852482 | MS.gene20686:CDS |
| GATGTGGAACCTCTTAAATT+TGG | 0.297774 | 1.2:-66857349 | MS.gene20686:CDS |
| TGGACGTTTGCCAGTTCTTC+AGG | 0.302967 | 1.2:-66857329 | MS.gene20686:intron |
| CTTTGTCTTGCAGGGTGTAT+TGG | 0.305421 | 1.2:-66854401 | MS.gene20686:intron |
| CCTTCCTACTTAATTATTGC+CGG | 0.308967 | 1.2:-66853484 | MS.gene20686:CDS |
| AGATTGCTATGCTGCTGTTT+TGG | 0.312140 | 1.2:-66852621 | MS.gene20686:CDS |
| CTCCTAGGTTTCCCTTGTCT+TGG | 0.319367 | 1.2:-66854930 | MS.gene20686:intron |
| TTTACCTGTCCTTTGAAATT+GGG | 0.327411 | 1.2:+66860894 | None:intergenic |
| TCTGAAGATGCTGAGAATAT+TGG | 0.329846 | 1.2:-66855478 | MS.gene20686:CDS |
| AGGAACGTGAAGATTGTATA+GGG | 0.339523 | 1.2:-66853294 | MS.gene20686:CDS |
| ATTCATTTGCAAGGTTTAAA+GGG | 0.357832 | 1.2:-66853147 | MS.gene20686:CDS |
| GCAGAACTTGGTATCGTTAG+AGG | 0.364713 | 1.2:-66853676 | MS.gene20686:CDS |
| TTTAAACCCACGAGTTCATT+TGG | 0.374117 | 1.2:-66853626 | MS.gene20686:intron |
| AAATGAGATACAACTGTTGA+TGG | 0.387290 | 1.2:+66855445 | None:intergenic |
| CTCCTTGATCGTTGAACGCT+GGG | 0.390026 | 1.2:+66856098 | None:intergenic |
| ACGATCAAGGAGAGTGCATT+GGG | 0.390362 | 1.2:-66856087 | MS.gene20686:CDS |
| ACAATGTTATACAGATTTGC+AGG | 0.393430 | 1.2:-66853706 | MS.gene20686:intron |
| TGCCAGGGAAGTGAATAATC+TGG | 0.393666 | 1.2:+66859102 | None:intergenic |
| AAGGATTCTGTAACGGTTGT+GGG | 0.394720 | 1.2:-66856522 | MS.gene20686:CDS |
| TCATCTTTGCTGAGAGACTA+TGG | 0.395374 | 1.2:-66852572 | MS.gene20686:CDS |
| GAAAAGAATGGCAAGTATAC+TGG | 0.395730 | 1.2:-66855409 | MS.gene20686:intron |
| CATGGATCGTCTGAGTTGTT+AGG | 0.397668 | 1.2:-66856346 | MS.gene20686:CDS |
| CTTTGCAGTGCGTTTATGAT+TGG | 0.400708 | 1.2:-66858977 | MS.gene20686:intron |
| TTGGCCACGACGGATTGCTC+TGG | 0.403272 | 1.2:-66852433 | MS.gene20686:CDS |
| CTGGGCTATCACCAAATTCC+TGG | 0.406265 | 1.2:+66852471 | None:intergenic |
| CTTGAAGACTTAAAAGAAGC+TGG | 0.416711 | 1.2:+66860618 | None:intergenic |
| TTCAAACATGCTCGCAGTGC+AGG | 0.417589 | 1.2:+66854882 | None:intergenic |
| GAAGGATTCTGTAACGGTTG+TGG | 0.421436 | 1.2:-66856523 | MS.gene20686:intron |
| CTATCACCAAATTCCTGGTC+TGG | 0.424623 | 1.2:+66852476 | None:intergenic |
| TCAAAAGAGGAAAGGTCTAC+TGG | 0.436520 | 1.2:-66860654 | MS.gene20686:CDS |
| GTTCCCTTTCATATTGATGA+AGG | 0.442609 | 1.2:-66853511 | MS.gene20686:CDS |
| GAGGAACGTGAAGATTGTAT+AGG | 0.444272 | 1.2:-66853295 | MS.gene20686:CDS |
| GGCAAACGTCCAAATTTAAG+AGG | 0.453895 | 1.2:+66857340 | None:intergenic |
| TGCAGTGCGTTTATGATTGG+AGG | 0.459995 | 1.2:-66858974 | MS.gene20686:intron |
| GTGGATTCACAAGAAGTAGA+AGG | 0.468763 | 1.2:-66852509 | MS.gene20686:CDS |
| CTGACTGACTACGAAAAGAA+TGG | 0.470639 | 1.2:-66855421 | MS.gene20686:CDS |
| CAGAGACAATTCACAAGCAC+TGG | 0.471481 | 1.2:-66859075 | MS.gene20686:CDS |
| ACATTATTTGCATCAGAAGT+TGG | 0.472154 | 1.2:+66854364 | None:intergenic |
| ATAAAGTACCTGCTGATCCT+TGG | 0.472858 | 1.2:+66860569 | None:intergenic |
| TCAATCAATGGTTCTGAAAG+AGG | 0.483299 | 1.2:+66853448 | None:intergenic |
| TCTCCTTGATCGTTGAACGC+TGG | 0.484829 | 1.2:+66856097 | None:intergenic |
| GCAGGTGATACTGCAGAACT+TGG | 0.490497 | 1.2:-66853688 | MS.gene20686:CDS |
| AATTCTGACCCCAATTTCAA+AGG | 0.495015 | 1.2:-66860903 | MS.gene20686:CDS |
| TAAATCACAACCAACGGCTC+TGG | 0.496717 | 1.2:+66857408 | None:intergenic |
| ACGGTTGTGGGATATCCTCT+TGG | 0.503638 | 1.2:-66856510 | MS.gene20686:CDS |
| ACTTCTTGTGAATCCACATA+GGG | 0.503832 | 1.2:+66852515 | None:intergenic |
| CTGACCTTCATCAATATGAA+AGG | 0.505131 | 1.2:+66853507 | None:intergenic |
| TTACCTGTCCTTTGAAATTG+GGG | 0.507312 | 1.2:+66860895 | None:intergenic |
| CAAATTCCTGGTCTGGTGGT+TGG | 0.509803 | 1.2:+66852483 | None:intergenic |
| TGACCCCAATTTCAAAGGAC+AGG | 0.510011 | 1.2:-66860898 | MS.gene20686:intron |
| ACAAATTGTAATTCTCTCAC+AGG | 0.514924 | 1.2:-66853122 | MS.gene20686:intron |
| GGCCCAGCGTTCAACGATCA+AGG | 0.515753 | 1.2:-66856100 | MS.gene20686:CDS |
| AATGCAACATACTCAATCAA+TGG | 0.517559 | 1.2:+66853436 | None:intergenic |
| CTCCAGATTATTCACTTCCC+TGG | 0.520748 | 1.2:-66859104 | MS.gene20686:CDS |
| ATTCTCTCATTTGAGCGGAA+TGG | 0.521849 | 1.2:+66854191 | None:intergenic |
| CAACTTCAAAATTTGAAACT+GGG | 0.523013 | 1.2:+66852453 | None:intergenic |
| CAGGTTACATCATATGCTCA+TGG | 0.532853 | 1.2:-66856364 | MS.gene20686:CDS |
| AGGTGACCAACCACCAGACC+AGG | 0.533734 | 1.2:-66852489 | MS.gene20686:CDS |
| TGGCCACGACGGATTGCTCT+GGG | 0.534074 | 1.2:-66852432 | MS.gene20686:CDS |
| AGAAGATAACACAGAGAACA+TGG | 0.534825 | 1.2:+66861019 | None:intergenic |
| TGAAGATATGAGCAATCAGC+AGG | 0.534944 | 1.2:-66852903 | MS.gene20686:intron |
| AACGATCAAGGAGAGTGCAT+TGG | 0.536494 | 1.2:-66856088 | MS.gene20686:CDS |
| AGGTTAAAGTCAAGAGAAGA+GGG | 0.538556 | 1.2:-66858481 | MS.gene20686:CDS |
| ATTGATGCAGCAATAAATCC+TGG | 0.539309 | 1.2:-66856316 | MS.gene20686:intron |
| TACTTCTTGTGAATCCACAT+AGG | 0.544023 | 1.2:+66852514 | None:intergenic |
| GCTGCAATGAATAGAAATGA+AGG | 0.544707 | 1.2:+66860941 | None:intergenic |
| TCACCAAATTCCTGGTCTGG+TGG | 0.545587 | 1.2:+66852479 | None:intergenic |
| TTGCAGATTATAAAATTCAA+CGG | 0.548933 | 1.2:-66852809 | MS.gene20686:intron |
| CAATGAGATGAGCAAGATGA+TGG | 0.549452 | 1.2:+66852768 | None:intergenic |
| GACACAATTTCAGTGACAAA+GGG | 0.549526 | 1.2:-66856483 | MS.gene20686:CDS |
| TGACTCGAAATATGTGGCTA+AGG | 0.550800 | 1.2:-66858456 | MS.gene20686:intron |
| TTGGGGTCAGAATTAGAAGA+AGG | 0.554085 | 1.2:+66860912 | None:intergenic |
| GTTGATGTTTCAGAATTCGT+TGG | 0.555603 | 1.2:-66860685 | MS.gene20686:intron |
| AGATCAGAAGATCTTTCAGA+TGG | 0.557002 | 1.2:+66852545 | None:intergenic |
| TCACATACCAAATGAACTCG+TGG | 0.559012 | 1.2:+66853619 | None:intergenic |
| AGGCACGTTATTCATTTGCA+AGG | 0.560334 | 1.2:-66853156 | MS.gene20686:CDS |
| TTGACTAATGAGGTAGTGAA+AGG | 0.560509 | 1.2:+66854166 | None:intergenic |
| AGATGACGATGCAGCAGCAA+CGG | 0.560811 | 1.2:+66852589 | None:intergenic |
| TAAGCCGGCAATAATTAAGT+AGG | 0.560984 | 1.2:+66853480 | None:intergenic |
| TTCATTTGCAAGGTTTAAAG+GGG | 0.564937 | 1.2:-66853146 | MS.gene20686:CDS |
| TTTACTTTCAGTAGAAAGCG+AGG | 0.565519 | 1.2:-66857383 | MS.gene20686:CDS |
| GTTGTGGGATATCCTCTTGG+AGG | 0.566876 | 1.2:-66856507 | MS.gene20686:CDS |
| TGACCTTCATCAATATGAAA+GGG | 0.569231 | 1.2:+66853508 | None:intergenic |
| TTGTTTGAAGGATTCTGTAA+CGG | 0.572193 | 1.2:-66856529 | MS.gene20686:intron |
| GGTAGACTCCAAGGATCAGC+AGG | 0.572458 | 1.2:-66860577 | MS.gene20686:intron |
| GGACTTTGTGGTAGACTCCA+AGG | 0.572501 | 1.2:-66860586 | MS.gene20686:CDS |
| GTTGGAGCTCTCATCAAAAG+AGG | 0.572525 | 1.2:-66860667 | MS.gene20686:CDS |
| CAGGTTAAAGTCAAGAGAAG+AGG | 0.577823 | 1.2:-66858482 | MS.gene20686:CDS |
| AGCTTTGATGATATACGTGT+TGG | 0.579456 | 1.2:-66854230 | MS.gene20686:CDS |
| TTTGAAACTACAGTCTAATG+AGG | 0.579854 | 1.2:-66854865 | MS.gene20686:intron |
| TCACCTTCACAACAGCATTG+AGG | 0.583189 | 1.2:+66859638 | None:intergenic |
| TTTCCTCAATGCTGTTGTGA+AGG | 0.584051 | 1.2:-66859641 | MS.gene20686:intron |
| GAAATGAAGGGTCTCTGTGT+AGG | 0.590316 | 1.2:+66860954 | None:intergenic |
| AGACACAATTTCAGTGACAA+AGG | 0.591490 | 1.2:-66856484 | MS.gene20686:CDS |
| TTGGCAAATGAGCTCAACAT+AGG | 0.592406 | 1.2:-66852929 | MS.gene20686:CDS |
| CACATACCAAATGAACTCGT+GGG | 0.593146 | 1.2:+66853620 | None:intergenic |
| TCAGCTAAAGTTATTGGCAA+AGG | 0.593749 | 1.2:-66853176 | MS.gene20686:intron |
| ATTAACCAACGCACATTGTG+TGG | 0.595162 | 1.2:-66858942 | MS.gene20686:CDS |
| TGCAAATAATGTATTAAAGG+AGG | 0.597855 | 1.2:-66854353 | MS.gene20686:intron |
| AAGGTCTACTGGAGGGAATG+TGG | 0.599448 | 1.2:-66860643 | MS.gene20686:CDS |
| AGCTCTCATCAAAAGAGGAA+AGG | 0.599990 | 1.2:-66860662 | MS.gene20686:CDS |
| AAGAGGAAAGGTCTACTGGA+GGG | 0.601207 | 1.2:-66860650 | MS.gene20686:CDS |
| TGTATAAGTACTCCAAGACA+AGG | 0.607018 | 1.2:+66854918 | None:intergenic |
| AAGAGGTGTGAACACTAAGC+CGG | 0.608861 | 1.2:+66853465 | None:intergenic |
| TAAAGCTAAATCACAACCAA+CGG | 0.613619 | 1.2:+66857402 | None:intergenic |
| GACAATTCACAAGCACTGGA+AGG | 0.615528 | 1.2:-66859071 | MS.gene20686:intron |
| AGGGGATGACTCGAAATATG+TGG | 0.616639 | 1.2:-66858462 | MS.gene20686:CDS |
| AGGCAATTCTCTCATTTGAG+CGG | 0.624954 | 1.2:+66854186 | None:intergenic |
| ATGCTCCACACAATGTGCGT+TGG | 0.628709 | 1.2:+66858937 | None:intergenic |
| GGAACGTGAAGATTGTATAG+GGG | 0.629382 | 1.2:-66853293 | MS.gene20686:intron |
| GATATACGTGTTGGATGTGA+AGG | 0.631547 | 1.2:-66854221 | MS.gene20686:CDS |
| CCGGCAATAATTAAGTAGGA+AGG | 0.633784 | 1.2:+66853484 | None:intergenic |
| GGGAGTTGTATCTCGTGTAG+AGG | 0.638533 | 1.2:-66856463 | MS.gene20686:intron |
| GAAATTGTGTCTCCTCCAAG+AGG | 0.643687 | 1.2:+66856495 | None:intergenic |
| CTGCAATGAATAGAAATGAA+GGG | 0.646164 | 1.2:+66860942 | None:intergenic |
| AAAGAGGAAAGGTCTACTGG+AGG | 0.649604 | 1.2:-66860651 | MS.gene20686:CDS |
| TCAAGGAGAGTGCATTGGGG+TGG | 0.650646 | 1.2:-66856083 | MS.gene20686:CDS |
| CAGAATTAGAAGAAGGAGTG+CGG | 0.665220 | 1.2:+66860919 | None:intergenic |
| GTATAAGTACTCCAAGACAA+GGG | 0.666945 | 1.2:+66854919 | None:intergenic |
| TTGTGTGGAGCATGATACAC+AGG | 0.667470 | 1.2:-66858927 | MS.gene20686:intron |
| GAACTTGGTATCGTTAGAGG+CGG | 0.672790 | 1.2:-66853673 | MS.gene20686:CDS |
| GAGTGTGTCTACGTGAGTAG+CGG | 0.691186 | 1.2:+66860982 | None:intergenic |
| ATGCCCAGAGCAATCCGTCG+TGG | 0.693119 | 1.2:+66852429 | None:intergenic |
| CGATCAAGGAGAGTGCATTG+GGG | 0.714890 | 1.2:-66856086 | MS.gene20686:CDS |
| GGTTAAAGTCAAGAGAAGAG+GGG | 0.719322 | 1.2:-66858480 | MS.gene20686:CDS |
| TGAGTAGCGGAATTTGACAG+TGG | 0.725425 | 1.2:+66860995 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 66852427 | 66861069 | 66852427 | ID=MS.gene20686 |
| chr1.2 | mRNA | 66852427 | 66861069 | 66852427 | ID=MS.gene20686.t1;Parent=MS.gene20686 |
| chr1.2 | exon | 66860899 | 66861069 | 66860899 | ID=MS.gene20686.t1.exon1;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66860899 | 66861069 | 66860899 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66860578 | 66860694 | 66860578 | ID=MS.gene20686.t1.exon2;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66860578 | 66860694 | 66860578 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66859642 | 66859692 | 66859642 | ID=MS.gene20686.t1.exon3;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66859642 | 66859692 | 66859642 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66859072 | 66859145 | 66859072 | ID=MS.gene20686.t1.exon4;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66859072 | 66859145 | 66859072 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66858928 | 66858991 | 66858928 | ID=MS.gene20686.t1.exon5;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66858928 | 66858991 | 66858928 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66858457 | 66858501 | 66858457 | ID=MS.gene20686.t1.exon6;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66858457 | 66858501 | 66858457 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66857330 | 66857437 | 66857330 | ID=MS.gene20686.t1.exon7;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66857330 | 66857437 | 66857330 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66856464 | 66856541 | 66856464 | ID=MS.gene20686.t1.exon8;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66856464 | 66856541 | 66856464 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66856317 | 66856383 | 66856317 | ID=MS.gene20686.t1.exon9;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66856317 | 66856383 | 66856317 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66856075 | 66856133 | 66856075 | ID=MS.gene20686.t1.exon10;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66856075 | 66856133 | 66856075 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66855410 | 66855509 | 66855410 | ID=MS.gene20686.t1.exon11;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66855410 | 66855509 | 66855410 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66854866 | 66854945 | 66854866 | ID=MS.gene20686.t1.exon12;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66854866 | 66854945 | 66854866 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66854354 | 66854410 | 66854354 | ID=MS.gene20686.t1.exon13;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66854354 | 66854410 | 66854354 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66854164 | 66854267 | 66854164 | ID=MS.gene20686.t1.exon14;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66854164 | 66854267 | 66854164 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66853627 | 66853714 | 66853627 | ID=MS.gene20686.t1.exon15;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66853627 | 66853714 | 66853627 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66853448 | 66853533 | 66853448 | ID=MS.gene20686.t1.exon16;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66853448 | 66853533 | 66853448 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66853294 | 66853318 | 66853294 | ID=MS.gene20686.t1.exon17;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66853294 | 66853318 | 66853294 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66853123 | 66853194 | 66853123 | ID=MS.gene20686.t1.exon18;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66853123 | 66853194 | 66853123 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66852904 | 66852954 | 66852904 | ID=MS.gene20686.t1.exon19;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66852904 | 66852954 | 66852904 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66852765 | 66852825 | 66852765 | ID=MS.gene20686.t1.exon20;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66852765 | 66852825 | 66852765 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| chr1.2 | exon | 66852427 | 66852677 | 66852427 | ID=MS.gene20686.t1.exon21;Parent=MS.gene20686.t1 |
| chr1.2 | CDS | 66852427 | 66852677 | 66852427 | ID=cds.MS.gene20686.t1;Parent=MS.gene20686.t1 |
| Gene Sequence |
| Protein sequence |
