AlfalfaGEDB | MS.gene21868

M. sativa cultivar XinJiangDaYe / MS.gene21868

KEGG: K00660
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009058 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00195.22 PF02797.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21868.t1	RHN53403.1	98.7	389	5	0	1	389	50	438	1.10E-221	778.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21868.t1	P30077	98.7	389	5	0	1	389	1	389	7.9e-224	777.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21868.t1	A0A072UNH2	98.7	389	5	0	1	389	1	389	8.1e-222	778.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene21868	-0.808859	2.52E-50	-1.69E-46
MS.gene049259	MS.gene21868	0.815256	1.01E-51	-1.69E-46
MS.gene049320	MS.gene21868	-0.803199	3.91E-49	-1.69E-46
MS.gene049483	MS.gene21868	0.821114	4.80E-53	-1.69E-46
MS.gene049549	MS.gene21868	0.812028	5.21E-51	-1.69E-46
MS.gene049611	MS.gene21868	0.840802	6.98E-58	-1.69E-46
MS.gene049634	MS.gene21868	0.819025	1.44E-52	-1.69E-46
MS.gene050082	MS.gene21868	0.804936	1.70E-49	-1.69E-46
MS.gene050216	MS.gene21868	-0.82091	5.34E-53	-1.69E-46
MS.gene050380	MS.gene21868	0.815818	7.61E-52	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	bit score
MS.gene21868.t1	MTR_5g007713	98.715	389	5	1	389	1	389	802
MS.gene21868.t1	MTR_5g007717	98.458	389	6	1	389	1	389	800
MS.gene21868.t1	MTR_1g098140	96.658	389	13	1	389	1	389	785
MS.gene21868.t1	MTR_1g097935	96.401	389	14	1	389	1	389	783
MS.gene21868.t1	MTR_5g007730	95.887	389	16	1	389	1	389	782
MS.gene21868.t1	MTR_1g097910	96.144	389	15	1	389	1	389	782
MS.gene21868.t1	MTR_7g016700	96.144	389	15	1	389	1	389	781
MS.gene21868.t1	MTR_7g016800	95.887	389	16	1	389	1	389	780
MS.gene21868.t1	MTR_7g016720	96.144	389	15	1	389	1	389	780
MS.gene21868.t1	MTR_7g016820	95.887	389	16	1	389	1	389	779

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21868.t1	AT5G13930	82.732	388	66	1	3	389	8	395	0.0	677
MS.gene21868.t1	AT4G34850	40.957	376	216	4	15	387	18	390	2.06e-96	293
MS.gene21868.t1	AT4G00040	38.520	392	229	6	1	387	1	385	1.23e-94	288
MS.gene21868.t1	AT1G02050	38.046	389	230	6	2	387	12	392	5.87e-86	266
MS.gene21868.t1	AT4G34850	40.752	319	184	3	72	387	1	317	3.59e-81	251
MS.gene21868.t1	AT3G52160	27.848	237	155	9	150	383	97	320	6.48e-12	66.6
MS.gene21868.t1	AT3G52160	27.848	237	155	9	150	383	210	433	1.24e-11	66.2

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Find 106 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGGTGCTTTGCAGGT+GGG	0.613935	5.1:+2052735	MS.gene21868:CDS
AACCTATATTTGAGATGGTT+TGG	0.308691	5.1:+2052954	MS.gene21868:CDS
AACGATTGCTCCAGACAGTG+AGG	0.649845	5.1:+2052985	MS.gene21868:CDS
AACTGTTGTTCTCCATAGTG+TGG	0.577442	5.1:+2053393	MS.gene21868:CDS
AAGTGTGTAATCTTTGATTT+TGG	0.277272	5.1:-2052611	None:intergenic
AATATAGGTTTCTCAATTTC+TGG	0.117134	5.1:-2052941	None:intergenic
AATCAGATATGTTTAATGGT+TGG	0.461034	5.1:-2053114	None:intergenic
AATGAGTATCACTAGGACCA+CGG	0.635157	5.1:-2052847	None:intergenic
ACAAGTGGTGTAGACATGCC+TGG	0.600112	5.1:+2052647	MS.gene21868:CDS
ACAGGAGAAGGGCTTGATTG+GGG	0.623900	5.1:+2053334	MS.gene21868:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCATATACCTCTTCACATA+TGG	-	chr5.1:2052710-2052729	None:intergenic	30.0%
TGAGAAACCTATATTTGAGA+TGG	+	chr5.1:2052949-2052968	MS.gene21868:CDS	30.0%
TTCAACTTGATCAAGAATTG+CGG	-	chr5.1:2053176-2053195	None:intergenic	30.0%
ACCTTCTTAAAGATGTTCCT+GGG	+	chr5.1:2053050-2053069	MS.gene21868:CDS	35.0%
AGATGAGATGCGAAAGAAAT+CGG	+	chr5.1:2053291-2053310	MS.gene21868:CDS	35.0%
AGGTATATGATGTACCAACA+AGG	+	chr5.1:2052719-2052738	MS.gene21868:CDS	35.0%
AGTGAAGGCCATAAAAGAAT+GGG	+	chr5.1:2052583-2052602	MS.gene21868:CDS	35.0%
ATGGAGAACAACAGTTTCAA+TGG	-	chr5.1:2053389-2053408	None:intergenic	35.0%
CATGTATCTTCTCTTGATCA+TGG	-	chr5.1:2052444-2052463	None:intergenic	35.0%
GGTATATGATGTACCAACAA+GGG	+	chr5.1:2052720-2052739	MS.gene21868:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	2052047	2053423	2052047	ID=MS.gene21868
chr5.1	mRNA	2052047	2053423	2052047	ID=MS.gene21868.t1;Parent=MS.gene21868
chr5.1	exon	2052047	2052224	2052047	ID=MS.gene21868.t1.exon1;Parent=MS.gene21868.t1
chr5.1	CDS	2052047	2052224	2052047	ID=cds.MS.gene21868.t1;Parent=MS.gene21868.t1
chr5.1	exon	2052432	2053423	2052432	ID=MS.gene21868.t1.exon2;Parent=MS.gene21868.t1
chr5.1	CDS	2052432	2053423	2052432	ID=cds.MS.gene21868.t1;Parent=MS.gene21868.t1

Gene Sequence

>MS.gene21868

ATGGTGAGTGTATCCGAAATTCGCAAGGCTCAAAGAGCAGAAGGCCCTGCAACCATCATGGCCATTGGCACTGCAAATCCAGCCAATTGTGTTGAACAAAGCACATATCCTGATTTCTACTTTAAAATCACAAATAGTGAGCACAAAACCGAGCTCAAAGAAAAATTTCAACGCATGTGTAAGTACTTTTTTGCATTATTATTTTTATGATCACAATAGTTAAATTACATATAACATGACACAAATGTTGTAGAATCACCCGTGATATGAAATTTGCCAACCAAATTATTTCAAACTATTTGCATTAAAAGACCTATTATTATTTGATTGGTTTTGGTATTACCCAAAAAAAATTGTACTAATGTAATTTCTTTAACTACTTCAGGTGATAAATCCATGATCAAGAGAAGATACATGTATCTTACCGAAGAGATTTTGAAAGAAAATCCAAGTGTATGTGAATACATGGCACCTTCATTGGATGCTAGGCAGGACATGGTGGTGGTAGAGGTACCTAGACTAGGAAAGGAGGCTGCAGTGAAGGCCATAAAAGAATGGGGCCAGCCAAAATCAAAGATTACACACTTGATCTTTTGTACCACAAGTGGTGTAGACATGCCTGGCGCCGATTACCAACTCACGAAACTCTTAGGTCTTCGTCCATATGTGAAGAGGTATATGATGTACCAACAAGGGTGCTTTGCAGGTGGGACGGTCCTTCGTTTGGCCAAGGACTTGGCTGAGAACAATAAAGGTGCTCGTGTGTTGGTTGTTTGTTCTGAAGTTACTGCTGTGACATTCCGTGGTCCTAGTGATACTCATTTGGACAGTCTTGTTGGTCAAGCACTCTTTGGAGATGGAGCTGCTGCGCTCATTGTTGGTTCTGACCCAATACCAGAAATTGAGAAACCTATATTTGAGATGGTTTGGACTGCACAAACGATTGCTCCAGACAGTGAGGGAGCCATTGACGGTCACCTTCGTGAAGCTGGTCTAACATTTCACCTTCTTAAAGATGTTCCTGGGATTGTTTCAAAGAACATTGATAAAGCATTAATTGAGGCTTTCCAACCATTAAACATATCTGATTACAACTCAATCTTTTGGATTGCTCACCCAGGTGGACCCGCAATTCTTGATCAAGTTGAACAAAAGTTAGGCTTAAAACCTGAAAAGATGAAAGCCACTAGAGAAGTACTTAGTGAATATGGTAACATGTCAAGTGCATGTGTATTGTTTATCTTAGATGAGATGCGAAAGAAATCGGCTCAACAGGGACTTAAAACCACAGGAGAAGGGCTTGATTGGGGTGTGTTGTTCGGCTTTGGACCTGGACTTACCATTGAAACTGTTGTTCTCCATAGTGTGGCTCTATGA

Protein sequence

>MS.gene21868.t1

MVSVSEIRKAQRAEGPATIMAIGTANPANCVEQSTYPDFYFKITNSEHKTELKEKFQRMCDKSMIKRRYMYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQALFGDGAAALIVGSDPIPEIEKPIFEMVWTAQTIAPDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGPAILDQVEQKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQQGLKTTGEGLDWGVLFGFGPGLTIETVVLHSVAL