Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31030.t1 | XP_003602501.1 | 96.1 | 333 | 13 | 0 | 1 | 333 | 1 | 333 | 6.10E-176 | 626.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31030.t1 | Q9LS48 | 46.1 | 89 | 48 | 0 | 218 | 306 | 212 | 300 | 5.5e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31030.t1 | G7JAC0 | 96.1 | 333 | 13 | 0 | 1 | 333 | 1 | 333 | 4.4e-176 | 626.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene31030 | 0.805863 | 1.09E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene31030 | 0.835694 | 1.45E-56 | -1.69E-46 |
| MS.gene049346 | MS.gene31030 | 0.804344 | 2.26E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene31030 | 0.875236 | 3.61E-68 | -1.69E-46 |
| MS.gene049427 | MS.gene31030 | 0.803256 | 3.80E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene31030 | 0.821957 | 3.06E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene31030 | 0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene31030 | 0.819252 | 1.28E-52 | -1.69E-46 |
| MS.gene049681 | MS.gene31030 | 0.814777 | 1.30E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene31030 | 0.831584 | 1.54E-55 | -1.69E-46 |
| MS.gene049910 | MS.gene31030 | 0.800292 | 1.54E-48 | -1.69E-46 |
| MS.gene050076 | MS.gene31030 | 0.826588 | 2.50E-54 | -1.69E-46 |
| MS.gene050099 | MS.gene31030 | 0.804095 | 2.55E-49 | -1.69E-46 |
| MS.gene050231 | MS.gene31030 | 0.844115 | 9.23E-59 | -1.69E-46 |
| MS.gene050301 | MS.gene31030 | 0.885831 | 5.79E-72 | -1.69E-46 |
| MS.gene050397 | MS.gene31030 | 0.825267 | 5.14E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene31030 | 0.8023 | 5.99E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene31030 | 0.810748 | 9.88E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene31030 | 0.845078 | 5.08E-59 | -1.69E-46 |
| MS.gene050560 | MS.gene31030 | 0.809742 | 1.63E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene31030 | 0.818259 | 2.15E-52 | -1.69E-46 |
| MS.gene050783 | MS.gene31030 | 0.81349 | 2.49E-51 | -1.69E-46 |
| MS.gene050812 | MS.gene31030 | 0.805991 | 1.02E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene31030 | 0.857051 | 2.12E-62 | -1.69E-46 |
| MS.gene051471 | MS.gene31030 | 0.816657 | 4.94E-52 | -1.69E-46 |
| MS.gene051525 | MS.gene31030 | 0.809709 | 1.66E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene31030 | 0.86528 | 6.62E-65 | -1.69E-46 |
| MS.gene051663 | MS.gene31030 | 0.825137 | 5.52E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene31030 | 0.860764 | 1.65E-63 | -1.69E-46 |
| MS.gene051877 | MS.gene31030 | 0.848032 | 7.93E-60 | -1.69E-46 |
| MS.gene052011 | MS.gene31030 | -0.832544 | 8.90E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene31030 | 0.847041 | 1.49E-59 | -1.69E-46 |
| MS.gene052178 | MS.gene31030 | 0.804664 | 1.94E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene31030 | 0.82174 | 3.44E-53 | -1.69E-46 |
| MS.gene052207 | MS.gene31030 | 0.819524 | 1.11E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene31030 | 0.867338 | 1.47E-65 | -1.69E-46 |
| MS.gene052310 | MS.gene31030 | 0.829337 | 5.45E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene31030 | 0.830766 | 2.44E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene31030 | 0.845337 | 4.33E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene31030 | 0.817743 | 2.81E-52 | -1.69E-46 |
| MS.gene052481 | MS.gene31030 | 0.8425 | 2.49E-58 | -1.69E-46 |
| MS.gene052485 | MS.gene31030 | 0.854391 | 1.27E-61 | -1.69E-46 |
| MS.gene053836 | MS.gene31030 | 0.890402 | 1.02E-73 | -1.69E-46 |
| MS.gene053971 | MS.gene31030 | 0.816656 | 4.94E-52 | -1.69E-46 |
| MS.gene054010 | MS.gene31030 | 0.81684 | 4.49E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene31030 | 0.834808 | 2.42E-56 | -1.69E-46 |
| MS.gene054012 | MS.gene31030 | 0.828015 | 1.14E-54 | -1.69E-46 |
| MS.gene054013 | MS.gene31030 | 0.821305 | 4.33E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene31030 | 0.810044 | 1.40E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene31030 | 0.823046 | 1.71E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene31030 | 0.880687 | 4.47E-70 | -1.69E-46 |
| MS.gene05455 | MS.gene31030 | 0.848903 | 4.56E-60 | -1.69E-46 |
| MS.gene054719 | MS.gene31030 | 0.828521 | 8.59E-55 | -1.69E-46 |
| MS.gene054802 | MS.gene31030 | 0.808838 | 2.54E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene31030 | 0.809222 | 2.10E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene31030 | 0.805339 | 1.40E-49 | -1.69E-46 |
| MS.gene054917 | MS.gene31030 | 0.804789 | 1.82E-49 | -1.69E-46 |
| MS.gene054918 | MS.gene31030 | 0.805184 | 1.51E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene31030 | 0.817769 | 2.78E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene31030 | 0.830188 | 3.38E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene31030 | 0.8348 | 2.43E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene31030 | 0.862975 | 3.46E-64 | -1.69E-46 |
| MS.gene055303 | MS.gene31030 | 0.81578 | 7.76E-52 | -1.69E-46 |
| MS.gene055390 | MS.gene31030 | 0.854041 | 1.60E-61 | -1.69E-46 |
| MS.gene055422 | MS.gene31030 | 0.877826 | 4.60E-69 | -1.69E-46 |
| MS.gene055428 | MS.gene31030 | 0.901937 | 1.62E-78 | -1.69E-46 |
| MS.gene05553 | MS.gene31030 | 0.808264 | 3.37E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene31030 | 0.846965 | 1.56E-59 | -1.69E-46 |
| MS.gene056191 | MS.gene31030 | 0.845576 | 3.73E-59 | -1.69E-46 |
| MS.gene056407 | MS.gene31030 | 0.84926 | 3.62E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene31030 | 0.820169 | 7.91E-53 | -1.69E-46 |
| MS.gene056451 | MS.gene31030 | 0.824823 | 6.55E-54 | -1.69E-46 |
| MS.gene056452 | MS.gene31030 | 0.837055 | 6.51E-57 | -1.69E-46 |
| MS.gene056497 | MS.gene31030 | 0.826805 | 2.22E-54 | -1.69E-46 |
| MS.gene056612 | MS.gene31030 | 0.840146 | 1.04E-57 | -1.69E-46 |
| MS.gene056723 | MS.gene31030 | 0.806833 | 6.79E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene31030 | 0.824549 | 7.60E-54 | -1.69E-46 |
| MS.gene056846 | MS.gene31030 | 0.807489 | 4.93E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene31030 | 0.863619 | 2.19E-64 | -1.69E-46 |
| MS.gene057121 | MS.gene31030 | 0.8799 | 8.54E-70 | -1.69E-46 |
| MS.gene057146 | MS.gene31030 | 0.807459 | 5.00E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene31030 | 0.854418 | 1.25E-61 | -1.69E-46 |
| MS.gene057295 | MS.gene31030 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene05733 | MS.gene31030 | 0.820609 | 6.27E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene31030 | 0.815834 | 7.54E-52 | -1.69E-46 |
| MS.gene057469 | MS.gene31030 | 0.820927 | 5.29E-53 | -1.69E-46 |
| MS.gene057749 | MS.gene31030 | 0.836162 | 1.10E-56 | -1.69E-46 |
| MS.gene057823 | MS.gene31030 | 0.825777 | 3.89E-54 | -1.69E-46 |
| MS.gene057825 | MS.gene31030 | 0.802005 | 6.89E-49 | -1.69E-46 |
| MS.gene057845 | MS.gene31030 | 0.801151 | 1.03E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene31030 | 0.885081 | 1.11E-71 | -1.69E-46 |
| MS.gene05811 | MS.gene31030 | 0.873346 | 1.58E-67 | -1.69E-46 |
| MS.gene058142 | MS.gene31030 | 0.843442 | 1.40E-58 | -1.69E-46 |
| MS.gene058233 | MS.gene31030 | 0.853652 | 2.07E-61 | -1.69E-46 |
| MS.gene058276 | MS.gene31030 | 0.82079 | 5.69E-53 | -1.69E-46 |
| MS.gene058293 | MS.gene31030 | 0.823719 | 1.19E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene31030 | 0.882429 | 1.05E-70 | -1.69E-46 |
| MS.gene058710 | MS.gene31030 | 0.811221 | 7.80E-51 | -1.69E-46 |
| MS.gene05886 | MS.gene31030 | 0.828229 | 1.01E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene31030 | 0.880967 | 3.55E-70 | -1.69E-46 |
| MS.gene059141 | MS.gene31030 | 0.845504 | 3.90E-59 | -1.69E-46 |
| MS.gene059294 | MS.gene31030 | 0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene31030 | 0.839357 | 1.66E-57 | -1.69E-46 |
| MS.gene059518 | MS.gene31030 | 0.839114 | 1.92E-57 | -1.69E-46 |
| MS.gene059536 | MS.gene31030 | 0.809609 | 1.74E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene31030 | 0.847306 | 1.26E-59 | -1.69E-46 |
| MS.gene059676 | MS.gene31030 | 0.809381 | 1.95E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene31030 | 0.843864 | 1.08E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene31030 | 0.843033 | 1.80E-58 | -1.69E-46 |
| MS.gene059695 | MS.gene31030 | 0.845438 | 4.06E-59 | -1.69E-46 |
| MS.gene05984 | MS.gene31030 | 0.854101 | 1.54E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene31030 | 0.82036 | 7.15E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene31030 | 0.819078 | 1.40E-52 | -1.69E-46 |
| MS.gene060403 | MS.gene31030 | 0.826983 | 2.01E-54 | -1.69E-46 |
| MS.gene060407 | MS.gene31030 | 0.806573 | 7.70E-50 | -1.69E-46 |
| MS.gene060559 | MS.gene31030 | 0.812539 | 4.03E-51 | -1.69E-46 |
| MS.gene06090 | MS.gene31030 | 0.815182 | 1.05E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene31030 | 0.860984 | 1.41E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene018541 | MS.gene31030 | PPI |
| MS.gene64503 | MS.gene31030 | PPI |
| MS.gene061914 | MS.gene31030 | PPI |
| MS.gene31030 | MS.gene09833 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31030.t1 | MTR_3g095070 | 96.096 | 333 | 13 | 0 | 1 | 333 | 1 | 333 | 0.0 | 660 |
| MS.gene31030.t1 | MTR_7g100328 | 37.011 | 281 | 152 | 6 | 60 | 324 | 74 | 345 | 3.27e-51 | 173 |
| MS.gene31030.t1 | MTR_1g067600 | 33.333 | 210 | 134 | 4 | 104 | 310 | 132 | 338 | 4.73e-26 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31030.t1 | AT4G39470 | 54.513 | 277 | 120 | 2 | 62 | 333 | 66 | 341 | 1.00e-102 | 305 |
| MS.gene31030.t1 | AT3G18420 | 36.992 | 246 | 142 | 4 | 74 | 306 | 55 | 300 | 2.36e-42 | 149 |
| MS.gene31030.t1 | AT2G37400 | 39.896 | 193 | 115 | 1 | 123 | 314 | 129 | 321 | 1.03e-37 | 137 |
| MS.gene31030.t1 | AT3G53560 | 40.000 | 195 | 112 | 2 | 123 | 314 | 129 | 321 | 2.01e-37 | 137 |
| MS.gene31030.t1 | AT3G53560 | 39.320 | 206 | 119 | 3 | 112 | 314 | 167 | 369 | 3.85e-37 | 137 |
| MS.gene31030.t1 | AT5G02590 | 39.691 | 194 | 115 | 2 | 115 | 307 | 120 | 312 | 6.30e-37 | 135 |
| MS.gene31030.t1 | AT3G09490 | 35.814 | 215 | 130 | 3 | 92 | 303 | 84 | 293 | 1.46e-30 | 118 |
Find 68 sgRNAs with CRISPR-Local
Find 146 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGATTGTATTTGGAAATTT+TGG | 0.111951 | 3.3:-78849738 | MS.gene31030:CDS |
| TGTTGATTCAGCTGAAATTT+TGG | 0.183578 | 3.3:-78849957 | MS.gene31030:CDS |
| TAAGGCTAAAGGATTGTATT+TGG | 0.219222 | 3.3:-78849747 | MS.gene31030:CDS |
| AACAAGTGCATAGTGATAAA+TGG | 0.231649 | 3.3:+78850131 | None:intergenic |
| ATACAGTCTTCTAGATAAAA+AGG | 0.263949 | 3.3:-78848023 | MS.gene31030:CDS |
| AGAAGGGAATTGGGAAGATT+TGG | 0.279074 | 3.3:+78850223 | None:intergenic |
| AAAATTGTGGAGGGTGTTAA+TGG | 0.334111 | 3.3:+78850100 | None:intergenic |
| TGGTTGTGTATGGGAAAATT+AGG | 0.344777 | 3.3:-78849886 | MS.gene31030:CDS |
| GTGTGCTTTGCAGGGTGATT+TGG | 0.355123 | 3.3:-78848315 | MS.gene31030:intron |
| CCCTTCTGTGGAAACTCTTC+AGG | 0.358106 | 3.3:+78847959 | None:intergenic |
| AGCCTCCACTCAACCTCATT+TGG | 0.359412 | 3.3:+78849809 | None:intergenic |
| TCGTGGCAAAAGAAGGGAAT+TGG | 0.399875 | 3.3:+78850213 | None:intergenic |
| ATTGTTGAAGTGAAATTGAT+AGG | 0.431111 | 3.3:+78850170 | None:intergenic |
| AATTCCTTCTGAAACTGTAC+TGG | 0.436247 | 3.3:+78847896 | None:intergenic |
| ACAAGTGCATAGTGATAAAT+GGG | 0.468344 | 3.3:+78850132 | None:intergenic |
| GCTCTTAAAGTGGTTGTGTA+TGG | 0.470707 | 3.3:-78849896 | MS.gene31030:CDS |
| TTGCAGGGTGATTTGGAGGA+GGG | 0.472903 | 3.3:-78848308 | MS.gene31030:intron |
| TTACGGAGGAGAGAAATATA+AGG | 0.473332 | 3.3:-78848477 | MS.gene31030:CDS |
| TACGAATTCGTGGCAAAAGA+AGG | 0.481369 | 3.3:+78850206 | None:intergenic |
| TTTGCAGGGTGATTTGGAGG+AGG | 0.482427 | 3.3:-78848309 | MS.gene31030:intron |
| TGCACAAATGCGTGTTGTAC+AGG | 0.487235 | 3.3:-78848446 | MS.gene31030:intron |
| TGCTTTGCAGGGTGATTTGG+AGG | 0.487354 | 3.3:-78848312 | MS.gene31030:intron |
| GATATCACCTTAGCAGCAAA+AGG | 0.497307 | 3.3:-78847926 | MS.gene31030:CDS |
| TTCAGCATTTCAAATACAGC+AGG | 0.497693 | 3.3:+78848535 | None:intergenic |
| GACAATGGGGTATTTGAGTA+AGG | 0.500527 | 3.3:-78849765 | MS.gene31030:CDS |
| CTCTTAAAGTGGTTGTGTAT+GGG | 0.502104 | 3.3:-78849895 | MS.gene31030:CDS |
| CGTGGCAAAAGAAGGGAATT+GGG | 0.502308 | 3.3:+78850214 | None:intergenic |
| AAGCTGAGAAGTTTGTGAAG+AGG | 0.509773 | 3.3:-78849847 | MS.gene31030:CDS |
| TTGTTGAAGTGAAATTGATA+GGG | 0.520583 | 3.3:+78850171 | None:intergenic |
| AAACTCACCTGGCAAAGATA+AGG | 0.536373 | 3.3:+78848236 | None:intergenic |
| GGGTATTTGAGTAAGGCTAA+AGG | 0.536389 | 3.3:-78849758 | MS.gene31030:CDS |
| GCATTGTGTTATGAGACAAT+GGG | 0.536548 | 3.3:-78849779 | MS.gene31030:CDS |
| GCCTGAAGAGTTTCCACAGA+AGG | 0.536929 | 3.3:-78847960 | MS.gene31030:CDS |
| AAATGTTGAGGCTCTTAAAG+TGG | 0.543541 | 3.3:-78849906 | MS.gene31030:CDS |
| TATTAATGAAAACAAAGTTA+CGG | 0.545367 | 3.3:-78848494 | MS.gene31030:CDS |
| GGTACTGAAACTGCAAAAGC+GGG | 0.549723 | 3.3:+78849983 | None:intergenic |
| CCTGAAGAGTTTCCACAGAA+GGG | 0.557973 | 3.3:-78847959 | MS.gene31030:CDS |
| AAGTTGGTTAAAGTTGTTTG+AGG | 0.558821 | 3.3:+78850073 | None:intergenic |
| GGAGGGTGTTAATGGAGTGC+GGG | 0.560063 | 3.3:+78850108 | None:intergenic |
| ATTCCTTCTGAAACTGTACT+GGG | 0.561008 | 3.3:+78847897 | None:intergenic |
| TGCATTGTGTTATGAGACAA+TGG | 0.562265 | 3.3:-78849780 | MS.gene31030:CDS |
| GATTGATGAGGAACCAAATG+AGG | 0.565692 | 3.3:-78849822 | MS.gene31030:CDS |
| TGGAGGGTGTTAATGGAGTG+CGG | 0.568184 | 3.3:+78850107 | None:intergenic |
| TCATCAAGAAATCCCTTCTG+TGG | 0.572197 | 3.3:+78847947 | None:intergenic |
| TGAAGAGCTCTAACAAGAAG+AGG | 0.582607 | 3.3:+78849707 | None:intergenic |
| AGGAACCAAATGAGGTTGAG+TGG | 0.584513 | 3.3:-78849814 | MS.gene31030:CDS |
| ACGAATTCGTGGCAAAAGAA+GGG | 0.586466 | 3.3:+78850207 | None:intergenic |
| TTATTGCAGATACGAATTCG+TGG | 0.587857 | 3.3:+78850196 | None:intergenic |
| AGGTACTGAAACTGCAAAAG+CGG | 0.602398 | 3.3:+78849982 | None:intergenic |
| TGTTGAAGTGAAATTGATAG+GGG | 0.604513 | 3.3:+78850172 | None:intergenic |
| TGTGAAGAGGTTGATTGATG+AGG | 0.608344 | 3.3:-78849834 | MS.gene31030:CDS |
| TGTTTGAGGTGAGAAAATTG+TGG | 0.610889 | 3.3:+78850087 | None:intergenic |
| ATCCCCAGTACAGTTTCAGA+AGG | 0.615032 | 3.3:-78847900 | MS.gene31030:CDS |
| GAAGGATTCAAGAAATGTTG+AGG | 0.622010 | 3.3:-78849918 | MS.gene31030:CDS |
| TGTATGGGAAAATTAGGAGA+GGG | 0.623027 | 3.3:-78849880 | MS.gene31030:CDS |
| ATTTCAGCTGAATCAACAGA+AGG | 0.625556 | 3.3:+78849962 | None:intergenic |
| TGAGGTGAGAAAATTGTGGA+GGG | 0.628555 | 3.3:+78850091 | None:intergenic |
| TGAGTAGATGAAGAGAGTGG+TGG | 0.640354 | 3.3:+78850255 | None:intergenic |
| TTCTGAGTAGATGAAGAGAG+TGG | 0.641757 | 3.3:+78850252 | None:intergenic |
| GTGTATGGGAAAATTAGGAG+AGG | 0.651978 | 3.3:-78849881 | MS.gene31030:CDS |
| AGATAAGGCCTAAAATCTCG+AGG | 0.652081 | 3.3:+78848251 | None:intergenic |
| TGTATTTGAAATGCTGAACA+AGG | 0.665874 | 3.3:-78848530 | MS.gene31030:CDS |
| TTGAGGTGAGAAAATTGTGG+AGG | 0.671928 | 3.3:+78850090 | None:intergenic |
| ATAGTGATAAATGGGTAGCA+CGG | 0.676425 | 3.3:+78850140 | None:intergenic |
| AACCAAATGAGGTTGAGTGG+AGG | 0.677766 | 3.3:-78849811 | MS.gene31030:CDS |
| TAATGAAAACAAAGTTACGG+AGG | 0.686188 | 3.3:-78848491 | MS.gene31030:CDS |
| TTCCTTCTGAAACTGTACTG+GGG | 0.733749 | 3.3:+78847898 | None:intergenic |
| CATTGTGTTATGAGACAATG+GGG | 0.737873 | 3.3:-78849778 | MS.gene31030:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAATGAAAACAAAGTTA+CGG | - | chr3.3:78849645-78849664 | MS.gene31030:intron | 15.0% |
| !!! | TAATACAATTACTATTTTGA+CGG | + | chr3.3:78849451-78849470 | None:intergenic | 15.0% |
| !!! | TCAAATTTTGATTAGAAATT+TGG | - | chr3.3:78848577-78848596 | MS.gene31030:intron | 15.0% |
| !! | AAATAAAGAGTTAAGAACTT+AGG | + | chr3.3:78849034-78849053 | None:intergenic | 20.0% |
| !! | AATAAAGAGTTAAGAACTTA+GGG | + | chr3.3:78849033-78849052 | None:intergenic | 20.0% |
| !! | CAAAACAATATAAGCATAAT+AGG | + | chr3.3:78849138-78849157 | None:intergenic | 20.0% |
| !! | CAATACAATAATTTCATGAA+TGG | + | chr3.3:78850064-78850083 | None:intergenic | 20.0% |
| !! | TTTATGTGATGAATATTAGA+CGG | - | chr3.3:78849207-78849226 | MS.gene31030:intron | 20.0% |
| !!! | AAAGTTTCATTTTTTCTCAT+TGG | - | chr3.3:78848099-78848118 | MS.gene31030:intron | 20.0% |
| !!! | AAGTTTCATTTTTTCTCATT+GGG | - | chr3.3:78848100-78848119 | MS.gene31030:intron | 20.0% |
| !!! | ACTAAGTTGAAAAGAATTTA+TGG | + | chr3.3:78848721-78848740 | None:intergenic | 20.0% |
| !!! | ATATGAATGCTTTTTTGTTT+AGG | - | chr3.3:78848550-78848569 | MS.gene31030:CDS | 20.0% |
| !!! | TAATATAGTTCTGTAAGATA+TGG | - | chr3.3:78850018-78850037 | MS.gene31030:CDS | 20.0% |
| !!! | TATATCTGTTTTTGTTTTGA+TGG | - | chr3.3:78848474-78848493 | MS.gene31030:CDS | 20.0% |
| !!! | TTTTGATTATGTTGAAAAGT+TGG | - | chr3.3:78848527-78848546 | MS.gene31030:CDS | 20.0% |
| ! | AATCTGAATCCAAAGTAAAA+TGG | + | chr3.3:78849347-78849366 | None:intergenic | 25.0% |
| ! | AATTGTCAAAATAACAGCTA+TGG | - | chr3.3:78849249-78849268 | MS.gene31030:intron | 25.0% |
| ! | AGAACAAAAATTAAAGTCAG+AGG | + | chr3.3:78849958-78849977 | None:intergenic | 25.0% |
| ! | ATACAGTCTTCTAGATAAAA+AGG | - | chr3.3:78850116-78850135 | MS.gene31030:CDS | 25.0% |
| ! | ATGCAATCATATTTGCATAA+AGG | + | chr3.3:78848982-78849001 | None:intergenic | 25.0% |
| ! | ATGTTAAAAGAATATGTTGC+AGG | - | chr3.3:78850086-78850105 | MS.gene31030:CDS | 25.0% |
| ! | ATTGTTGAAGTGAAATTGAT+AGG | + | chr3.3:78847972-78847991 | None:intergenic | 25.0% |
| ! | TCACATAAATTTACAATCAC+AGG | + | chr3.3:78849196-78849215 | None:intergenic | 25.0% |
| ! | TGTTAAAAGAATATGTTGCA+GGG | - | chr3.3:78850087-78850106 | MS.gene31030:CDS | 25.0% |
| ! | TTGTTGAAGTGAAATTGATA+GGG | + | chr3.3:78847971-78847990 | None:intergenic | 25.0% |
| ! | TTTGATGGAATTATGAATGT+TGG | - | chr3.3:78848489-78848508 | MS.gene31030:CDS | 25.0% |
| !! | AACTTTTTGTGAAATGAAGT+TGG | + | chr3.3:78848085-78848104 | None:intergenic | 25.0% |
| !! | AGGATTGTATTTGGAAATTT+TGG | - | chr3.3:78848401-78848420 | MS.gene31030:intron | 25.0% |
| !!! | ACATTGATAATTGTTGACTT+AGG | - | chr3.3:78848613-78848632 | MS.gene31030:intron | 25.0% |
| !!! | AGTTGTGAGAATGTTTTAAA+GGG | - | chr3.3:78848670-78848689 | MS.gene31030:intron | 25.0% |
| !!! | ATAGCTGTTATTTTGACAAT+TGG | + | chr3.3:78849250-78849269 | None:intergenic | 25.0% |
| !!! | ATTTGGAAATTTTGGAGAAT+TGG | - | chr3.3:78848409-78848428 | MS.gene31030:intron | 25.0% |
| !!! | CATTGATAATTGTTGACTTA+GGG | - | chr3.3:78848614-78848633 | MS.gene31030:intron | 25.0% |
| !!! | GTCTTTTTGGAGTAATTATT+TGG | - | chr3.3:78849787-78849806 | MS.gene31030:CDS | 25.0% |
| !!! | TAGTTGTGAGAATGTTTTAA+AGG | - | chr3.3:78848669-78848688 | MS.gene31030:intron | 25.0% |
| !!! | TGTTTTTTCATAAGTTATCG+TGG | + | chr3.3:78848756-78848775 | None:intergenic | 25.0% |
| AACAAGTGCATAGTGATAAA+TGG | + | chr3.3:78848011-78848030 | None:intergenic | 30.0% | |
| AACATCCAGAATTCATACAT+GGG | + | chr3.3:78849088-78849107 | None:intergenic | 30.0% | |
| AATATGATTGCATGTTGACA+TGG | - | chr3.3:78848989-78849008 | MS.gene31030:intron | 30.0% | |
| ACAAGTGCATAGTGATAAAT+GGG | + | chr3.3:78848010-78848029 | None:intergenic | 30.0% | |
| TAAAACCCATGTATGAATTC+TGG | - | chr3.3:78849080-78849099 | MS.gene31030:intron | 30.0% | |
| TAAGGCTAAAGGATTGTATT+TGG | - | chr3.3:78848392-78848411 | MS.gene31030:intron | 30.0% | |
| TAATGAAAACAAAGTTACGG+AGG | - | chr3.3:78849648-78849667 | MS.gene31030:intron | 30.0% | |
| TACTTTGGATTCAGATTTGA+AGG | - | chr3.3:78849350-78849369 | MS.gene31030:intron | 30.0% | |
| TGTCATGAAATCAATTTACC+AGG | - | chr3.3:78849479-78849498 | MS.gene31030:intron | 30.0% | |
| TGTTGAAGTGAAATTGATAG+GGG | + | chr3.3:78847970-78847989 | None:intergenic | 30.0% | |
| TTGAGCCTTAGTATGTATTT+CGG | - | chr3.3:78849163-78849182 | MS.gene31030:intron | 30.0% | |
| ! | AACCATGAACAATGTCTTTT+TGG | - | chr3.3:78849774-78849793 | MS.gene31030:CDS | 30.0% |
| ! | AAGTTGGTTAAAGTTGTTTG+AGG | + | chr3.3:78848069-78848088 | None:intergenic | 30.0% |
| ! | ATCAAAATCCTCGAGATTTT+AGG | - | chr3.3:78849880-78849899 | MS.gene31030:CDS | 30.0% |
| ! | GGTAAATTGATTTCATGACA+TGG | + | chr3.3:78849479-78849498 | None:intergenic | 30.0% |
| ! | TGTATTTGAAATGCTGAACA+AGG | - | chr3.3:78849609-78849628 | MS.gene31030:intron | 30.0% |
| !! | AATTTTGGAGGAGAAGATTT+TGG | - | chr3.3:78848197-78848216 | MS.gene31030:intron | 30.0% |
| !!! | TGTTGATTCAGCTGAAATTT+TGG | - | chr3.3:78848182-78848201 | MS.gene31030:intron | 30.0% |
| AAATGTTGAGGCTCTTAAAG+TGG | - | chr3.3:78848233-78848252 | MS.gene31030:intron | 35.0% | |
| AACAAACAAATTGAGTGTCC+TGG | + | chr3.3:78849500-78849519 | None:intergenic | 35.0% | |
| AACTCGTAGTAGATAAGCAA+AGG | + | chr3.3:78849747-78849766 | None:intergenic | 35.0% | |
| ATAACATATGTGTGCTTTGC+AGG | - | chr3.3:78849815-78849834 | MS.gene31030:CDS | 35.0% | |
| ATAGTGATAAATGGGTAGCA+CGG | + | chr3.3:78848002-78848021 | None:intergenic | 35.0% | |
| ATTTCAGCTGAATCAACAGA+AGG | + | chr3.3:78848180-78848199 | None:intergenic | 35.0% | |
| CAACATCCAGAATTCATACA+TGG | + | chr3.3:78849089-78849108 | None:intergenic | 35.0% | |
| CAGTCTTCTAGATAAAAAGG+AGG | - | chr3.3:78850119-78850138 | MS.gene31030:CDS | 35.0% | |
| CATTGTGTTATGAGACAATG+GGG | - | chr3.3:78848361-78848380 | MS.gene31030:intron | 35.0% | |
| CTCCAAAAAGACATTGTTCA+TGG | + | chr3.3:78849779-78849798 | None:intergenic | 35.0% | |
| CTCTTAAAGTGGTTGTGTAT+GGG | - | chr3.3:78848244-78848263 | MS.gene31030:CDS | 35.0% | |
| CTGAATCCAAAGTAAAATGG+CGG | + | chr3.3:78849344-78849363 | None:intergenic | 35.0% | |
| GAAGGATTCAAGAAATGTTG+AGG | - | chr3.3:78848221-78848240 | MS.gene31030:intron | 35.0% | |
| GCATTGTGTTATGAGACAAT+GGG | - | chr3.3:78848360-78848379 | MS.gene31030:intron | 35.0% | |
| GTGATGCACAAAAACAATGA+GGG | - | chr3.3:78849580-78849599 | MS.gene31030:intron | 35.0% | |
| TAACATATGTGTGCTTTGCA+GGG | - | chr3.3:78849816-78849835 | MS.gene31030:CDS | 35.0% | |
| TCTTCCCGAAATACATACTA+AGG | + | chr3.3:78849171-78849190 | None:intergenic | 35.0% | |
| TGAGCCTTAGTATGTATTTC+GGG | - | chr3.3:78849164-78849183 | MS.gene31030:intron | 35.0% | |
| TGCATTGTGTTATGAGACAA+TGG | - | chr3.3:78848359-78848378 | MS.gene31030:intron | 35.0% | |
| TGGTTGTGTATGGGAAAATT+AGG | - | chr3.3:78848253-78848272 | MS.gene31030:CDS | 35.0% | |
| TGTATGGGAAAATTAGGAGA+GGG | - | chr3.3:78848259-78848278 | MS.gene31030:CDS | 35.0% | |
| TTACGGAGGAGAGAAATATA+AGG | - | chr3.3:78849662-78849681 | MS.gene31030:intron | 35.0% | |
| TTAGTATGTATTTCGGGAAG+AGG | - | chr3.3:78849170-78849189 | MS.gene31030:intron | 35.0% | |
| TTATTGCAGATACGAATTCG+TGG | + | chr3.3:78847946-78847965 | None:intergenic | 35.0% | |
| TTCAGCATTTCAAATACAGC+AGG | + | chr3.3:78849607-78849626 | None:intergenic | 35.0% | |
| TTTGCTAGATGCTTCTTTGA+CGG | - | chr3.3:78849376-78849395 | MS.gene31030:intron | 35.0% | |
| TTTGGATTCAGATTTGAAGG+TGG | - | chr3.3:78849353-78849372 | MS.gene31030:intron | 35.0% | |
| ! | AAACAAGTTGTGTTGCTGAT+TGG | - | chr3.3:78848880-78848899 | MS.gene31030:intron | 35.0% |
| ! | AACTGTGCAGTGTTGTTAAA+TGG | - | chr3.3:78849277-78849296 | MS.gene31030:intron | 35.0% |
| ! | AGTGATACTCTCTTTTCTAC+AGG | - | chr3.3:78849546-78849565 | MS.gene31030:intron | 35.0% |
| ! | GATTGGTAATCATGGCTATT+AGG | - | chr3.3:78848897-78848916 | MS.gene31030:intron | 35.0% |
| ! | GTGATACTCTCTTTTCTACA+GGG | - | chr3.3:78849547-78849566 | MS.gene31030:intron | 35.0% |
| ! | TGATTCAGCTGAAATTTTGG+AGG | - | chr3.3:78848185-78848204 | MS.gene31030:intron | 35.0% |
| ! | TGTTTGAGGTGAGAAAATTG+TGG | + | chr3.3:78848055-78848074 | None:intergenic | 35.0% |
| !! | AAAATTGTGGAGGGTGTTAA+TGG | + | chr3.3:78848042-78848061 | None:intergenic | 35.0% |
| !! | AATTCCTTCTGAAACTGTAC+TGG | + | chr3.3:78850246-78850265 | None:intergenic | 35.0% |
| !! | ATTCCTTCTGAAACTGTACT+GGG | + | chr3.3:78850245-78850264 | None:intergenic | 35.0% |
| !!! | ACTATTTTGACGGCATTTTG+TGG | + | chr3.3:78849441-78849460 | None:intergenic | 35.0% |
| AAACTCACCTGGCAAAGATA+AGG | + | chr3.3:78849906-78849925 | None:intergenic | 40.0% | |
| AAGCTGAGAAGTTTGTGAAG+AGG | - | chr3.3:78848292-78848311 | MS.gene31030:CDS | 40.0% | |
| AAGTTATCGTGGAGAGCTTA+TGG | + | chr3.3:78848745-78848764 | None:intergenic | 40.0% | |
| ACGAATTCGTGGCAAAAGAA+GGG | + | chr3.3:78847935-78847954 | None:intergenic | 40.0% | |
| AGAAGGGAATTGGGAAGATT+TGG | + | chr3.3:78847919-78847938 | None:intergenic | 40.0% | |
| AGATAAGGCCTAAAATCTCG+AGG | + | chr3.3:78849891-78849910 | None:intergenic | 40.0% | |
| GATATCACCTTAGCAGCAAA+AGG | - | chr3.3:78850213-78850232 | MS.gene31030:CDS | 40.0% | |
| GATTGATGAGGAACCAAATG+AGG | - | chr3.3:78848317-78848336 | MS.gene31030:intron | 40.0% | |
| GATTTGCATACAAACTCACC+TGG | + | chr3.3:78849917-78849936 | None:intergenic | 40.0% | |
| GCTCTTAAAGTGGTTGTGTA+TGG | - | chr3.3:78848243-78848262 | MS.gene31030:intron | 40.0% | |
| GGGTATTTGAGTAAGGCTAA+AGG | - | chr3.3:78848381-78848400 | MS.gene31030:intron | 40.0% | |
| GGTGATGCACAAAAACAATG+AGG | - | chr3.3:78849579-78849598 | MS.gene31030:intron | 40.0% | |
| GTGTATGGGAAAATTAGGAG+AGG | - | chr3.3:78848258-78848277 | MS.gene31030:CDS | 40.0% | |
| TACGAATTCGTGGCAAAAGA+AGG | + | chr3.3:78847936-78847955 | None:intergenic | 40.0% | |
| TATGACGCTTGTCAAAACAC+CGG | + | chr3.3:78849314-78849333 | None:intergenic | 40.0% | |
| TCATCAAGAAATCCCTTCTG+TGG | + | chr3.3:78850195-78850214 | None:intergenic | 40.0% | |
| TGAGGTGAGAAAATTGTGGA+GGG | + | chr3.3:78848051-78848070 | None:intergenic | 40.0% | |
| TTCTGAGTAGATGAAGAGAG+TGG | + | chr3.3:78847890-78847909 | None:intergenic | 40.0% | |
| TTGAGGTGAGAAAATTGTGG+AGG | + | chr3.3:78848052-78848071 | None:intergenic | 40.0% | |
| ! | ACTCTCTTTTCTACAGGGTT+TGG | - | chr3.3:78849552-78849571 | MS.gene31030:intron | 40.0% |
| ! | GACAATGGGGTATTTGAGTA+AGG | - | chr3.3:78848374-78848393 | MS.gene31030:intron | 40.0% |
| ! | GTGTTGCTGATTGGTAATCA+TGG | - | chr3.3:78848889-78848908 | MS.gene31030:intron | 40.0% |
| ! | TGAAGAGCTCTAACAAGAAG+AGG | + | chr3.3:78848435-78848454 | None:intergenic | 40.0% |
| ! | TGTGAAGAGGTTGATTGATG+AGG | - | chr3.3:78848305-78848324 | MS.gene31030:CDS | 40.0% |
| ! | TTTTCTACAGGGTTTGGCTA+TGG | - | chr3.3:78849558-78849577 | MS.gene31030:intron | 40.0% |
| !! | AGGTACTGAAACTGCAAAAG+CGG | + | chr3.3:78848160-78848179 | None:intergenic | 40.0% |
| !! | TGAACACCGCCATTTTACTT+TGG | - | chr3.3:78849335-78849354 | MS.gene31030:intron | 40.0% |
| !! | TTCCTTCTGAAACTGTACTG+GGG | + | chr3.3:78850244-78850263 | None:intergenic | 40.0% |
| !! | TTGTTGACTTAGGGTCTGTT+TGG | - | chr3.3:78848623-78848642 | MS.gene31030:intron | 40.0% |
| !! | TTTTAGGCCTTATCTTTGCC+AGG | - | chr3.3:78849896-78849915 | MS.gene31030:CDS | 40.0% |
| AACCAAATGAGGTTGAGTGG+AGG | - | chr3.3:78848328-78848347 | MS.gene31030:intron | 45.0% | |
| AGGAACCAAATGAGGTTGAG+TGG | - | chr3.3:78848325-78848344 | MS.gene31030:intron | 45.0% | |
| ATCCCCAGTACAGTTTCAGA+AGG | - | chr3.3:78850239-78850258 | MS.gene31030:CDS | 45.0% | |
| CCTGAAGAGTTTCCACAGAA+GGG | - | chr3.3:78850180-78850199 | MS.gene31030:CDS | 45.0% | |
| CGTGGCAAAAGAAGGGAATT+GGG | + | chr3.3:78847928-78847947 | None:intergenic | 45.0% | |
| GATGAATATTAGACGGCGCT+AGG | - | chr3.3:78849214-78849233 | MS.gene31030:intron | 45.0% | |
| TCGTGGCAAAAGAAGGGAAT+TGG | + | chr3.3:78847929-78847948 | None:intergenic | 45.0% | |
| TGAGTAGATGAAGAGAGTGG+TGG | + | chr3.3:78847887-78847906 | None:intergenic | 45.0% | |
| TGCACAAATGCGTGTTGTAC+AGG | - | chr3.3:78849693-78849712 | MS.gene31030:intron | 45.0% | |
| ! | GCTTCTTTGACGGCATTTTG+TGG | - | chr3.3:78849386-78849405 | MS.gene31030:intron | 45.0% |
| ! | GGATGTTCCTTTTGCTGCTA+AGG | + | chr3.3:78850223-78850242 | None:intergenic | 45.0% |
| ! | GGTACTGAAACTGCAAAAGC+GGG | + | chr3.3:78848159-78848178 | None:intergenic | 45.0% |
| !! | GGAGGAGAAGATTTTGGAGA+AGG | - | chr3.3:78848203-78848222 | MS.gene31030:intron | 45.0% |
| AGCCTCCACTCAACCTCATT+TGG | + | chr3.3:78848333-78848352 | None:intergenic | 50.0% | |
| CCCTTCTGTGGAAACTCTTC+AGG | + | chr3.3:78850183-78850202 | None:intergenic | 50.0% | |
| GCCTGAAGAGTTTCCACAGA+AGG | - | chr3.3:78850179-78850198 | MS.gene31030:CDS | 50.0% | |
| GCTTGTCAAAACACCGGTCA+CGG | + | chr3.3:78849308-78849327 | None:intergenic | 50.0% | |
| GTGTGCTTTGCAGGGTGATT+TGG | - | chr3.3:78849824-78849843 | MS.gene31030:CDS | 50.0% | |
| ! | TGCTTTGCAGGGTGATTTGG+AGG | - | chr3.3:78849827-78849846 | MS.gene31030:CDS | 50.0% |
| ! | TTGCAGGGTGATTTGGAGGA+GGG | - | chr3.3:78849831-78849850 | MS.gene31030:CDS | 50.0% |
| ! | TTTGCAGGGTGATTTGGAGG+AGG | - | chr3.3:78849830-78849849 | MS.gene31030:CDS | 50.0% |
| !! | TGGAGGGTGTTAATGGAGTG+CGG | + | chr3.3:78848035-78848054 | None:intergenic | 50.0% |
| TTAAATGGCGACGCCGTGAC+CGG | - | chr3.3:78849292-78849311 | MS.gene31030:intron | 55.0% | |
| !! | GGAGGGTGTTAATGGAGTGC+GGG | + | chr3.3:78848034-78848053 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 78847868 | 78850293 | 78847868 | ID=MS.gene31030 |
| chr3.3 | mRNA | 78847868 | 78850293 | 78847868 | ID=MS.gene31030.t1;Parent=MS.gene31030 |
| chr3.3 | exon | 78849706 | 78850293 | 78849706 | ID=MS.gene31030.t1.exon1;Parent=MS.gene31030.t1 |
| chr3.3 | CDS | 78849706 | 78850293 | 78849706 | ID=cds.MS.gene31030.t1;Parent=MS.gene31030.t1 |
| chr3.3 | exon | 78848447 | 78848593 | 78848447 | ID=MS.gene31030.t1.exon2;Parent=MS.gene31030.t1 |
| chr3.3 | CDS | 78848447 | 78848593 | 78848447 | ID=cds.MS.gene31030.t1;Parent=MS.gene31030.t1 |
| chr3.3 | exon | 78848244 | 78848324 | 78848244 | ID=MS.gene31030.t1.exon3;Parent=MS.gene31030.t1 |
| chr3.3 | CDS | 78848244 | 78848324 | 78848244 | ID=cds.MS.gene31030.t1;Parent=MS.gene31030.t1 |
| chr3.3 | exon | 78847868 | 78848053 | 78847868 | ID=MS.gene31030.t1.exon4;Parent=MS.gene31030.t1 |
| chr3.3 | CDS | 78847868 | 78848053 | 78847868 | ID=cds.MS.gene31030.t1;Parent=MS.gene31030.t1 |
| Gene Sequence |
| Protein sequence |
