AlfalfaGEDB | MS.gene32271

M. sativa cultivar XinJiangDaYe / MS.gene32271

KEGG: K07305
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016667 GO:0016671 GO:0033743 GO:0055114
PFAM: PF01641.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32271.t1	AFK39115.1	94.1	205	9	2	1	203	1	204	1.20E-102	382.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32271.t1	Q9C8M2	72.0	157	39	2	43	199	43	194	6.2e-63	241.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32271.t1	I3SN78	94.1	205	9	2	1	203	1	204	8.9e-103	382.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene32271	0.838847	2.25E-57	-1.69E-46
MS.gene049222	MS.gene32271	0.822341	2.49E-53	-1.69E-46
MS.gene049285	MS.gene32271	0.829406	5.24E-55	-1.69E-46
MS.gene049320	MS.gene32271	0.808354	3.23E-50	-1.69E-46
MS.gene049609	MS.gene32271	0.800806	1.21E-48	-1.69E-46
MS.gene049633	MS.gene32271	0.879177	1.54E-69	-1.69E-46
MS.gene050170	MS.gene32271	0.867033	1.84E-65	-1.69E-46
MS.gene050323	MS.gene32271	0.863035	3.32E-64	-1.69E-46
MS.gene050338	MS.gene32271	-0.851801	7.00E-61	-1.69E-46
MS.gene050444	MS.gene32271	0.846855	1.67E-59	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene027087	MS.gene32271	PPI
MS.gene30166	MS.gene32271	PPI
MS.gene82727	MS.gene32271	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32271.t1	MTR_8g461030	94.764	191	9	1	13	203	15	204	8.00e-128	358
MS.gene32271.t1	MTR_4g092800	45.600	125	63	2	79	201	65	186	9.47e-34	119
MS.gene32271.t1	MTR_2g020930	43.200	125	66	2	79	201	11	132	7.06e-31	110
MS.gene32271.t1	MTR_2g020950	40.164	122	68	2	82	201	13	131	1.54e-26	99.4
MS.gene32271.t1	MTR_2g020900	40.594	101	51	3	105	201	47	142	6.08e-19	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32271.t1	AT1G53670	80.159	126	25	0	74	199	69	194	5.59e-80	237
MS.gene32271.t1	AT1G53670	75.397	126	24	1	74	199	69	187	2.98e-73	220
MS.gene32271.t1	AT4G04800	44.000	125	65	2	79	201	50	171	4.25e-33	117
MS.gene32271.t1	AT4G04830	40.945	127	70	2	79	203	11	134	4.31e-31	110
MS.gene32271.t1	AT4G21840	44.262	122	63	2	80	199	18	136	6.39e-31	110
MS.gene32271.t1	AT4G21830	44.262	122	63	2	80	199	16	134	7.74e-31	110
MS.gene32271.t1	AT4G21830	44.262	122	63	2	80	199	19	137	1.36e-30	109
MS.gene32271.t1	AT4G21860	40.580	138	77	2	66	201	63	197	3.87e-30	110
MS.gene32271.t1	AT4G21860	40.580	138	77	2	66	201	63	197	3.87e-30	110
MS.gene32271.t1	AT4G21860	40.580	138	77	2	66	201	63	197	3.87e-30	110

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Find 42 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTTCTTCTTCAATCC+GGG	0.445114	8.3:+48852132	MS.gene32271:CDS
AAACAGCACAGAGAACTTCT+TGG	0.444117	8.3:-48855061	None:intergenic
AAATTTGTGAATTTGGGTAT+TGG	0.367865	8.3:-48852056	None:intergenic
AACACTACAGAAATTCAATC+AGG	0.493813	8.3:+48852193	MS.gene32271:CDS
AAGCTGAAGAGCCCATTGCC+CGG	0.557701	8.3:-48852150	None:intergenic
AATCTTTAAGTGATGCAGAA+TGG	0.513509	8.3:+48853677	MS.gene32271:CDS
AATTTGTGAATTTGGGTATT+GGG	0.342711	8.3:-48852055	None:intergenic
ACCAAGTTTAATAGTGGAAC+TGG	0.412251	8.3:+48854963	MS.gene32271:CDS
ACCAGTTCCACTATTAAACT+TGG	0.499953	8.3:-48854964	None:intergenic
ACTGGAATACCAAAACTGAA+GGG	0.657265	8.3:+48854768	MS.gene32271:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTTTCTTCTTCAATCC+GGG	+	chr8.3:48852132-48852151	MS.gene32271:CDS	30.0%
AACACACAATCATTGAAAGA+AGG	+	chr8.3:48854123-48854142	MS.gene32271:intron	30.0%
AACACTACAGAAATTCAATC+AGG	+	chr8.3:48852193-48852212	MS.gene32271:CDS	30.0%
AACATACATGTAACGTTTAG+AGG	+	chr8.3:48853347-48853366	MS.gene32271:intron	30.0%
AATCTTTAAGTGATGCAGAA+TGG	+	chr8.3:48853677-48853696	MS.gene32271:CDS	30.0%
ACATACATGTAACGTTTAGA+GGG	+	chr8.3:48853348-48853367	MS.gene32271:intron	30.0%
ACATTGCCCTTAATCATATA+TGG	-	chr8.3:48854619-48854638	None:intergenic	30.0%
AGAAGGGAAAAAAAATCTAG+TGG	+	chr8.3:48852411-48852430	MS.gene32271:intron	30.0%
ATATGATTAAGGGCAATGTA+AGG	+	chr8.3:48854620-48854639	MS.gene32271:intron	30.0%
ATCCTTGTATCTTTCATTTC+AGG	-	chr8.3:48854362-48854381	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	48852010	48855273	48852010	ID=MS.gene32271
chr8.3	mRNA	48852010	48855273	48852010	ID=MS.gene32271.t1;Parent=MS.gene32271
chr8.3	exon	48852010	48852214	48852010	ID=MS.gene32271.t1.exon1;Parent=MS.gene32271.t1
chr8.3	CDS	48852010	48852214	48852010	ID=cds.MS.gene32271.t1;Parent=MS.gene32271.t1
chr8.3	exon	48853656	48853767	48853656	ID=MS.gene32271.t1.exon2;Parent=MS.gene32271.t1
chr8.3	CDS	48853656	48853767	48853656	ID=cds.MS.gene32271.t1;Parent=MS.gene32271.t1
chr8.3	exon	48854763	48854828	48854763	ID=MS.gene32271.t1.exon3;Parent=MS.gene32271.t1
chr8.3	CDS	48854763	48854828	48854763	ID=cds.MS.gene32271.t1;Parent=MS.gene32271.t1
chr8.3	exon	48854956	48855153	48854956	ID=MS.gene32271.t1.exon4;Parent=MS.gene32271.t1
chr8.3	CDS	48854956	48855153	48854956	ID=cds.MS.gene32271.t1;Parent=MS.gene32271.t1
chr8.3	exon	48855243	48855273	48855243	ID=MS.gene32271.t1.exon5;Parent=MS.gene32271.t1
chr8.3	CDS	48855243	48855273	48855243	ID=cds.MS.gene32271.t1;Parent=MS.gene32271.t1

Gene Sequence

>MS.gene32271

ATGGCATCTCATAGTTTGAGCCTATCAGCTGCTGCACAAATTCTTCCCAATACCCAAATTCACAAATTTGATTCTAAACTTTTGTTGTGGCCATCGCGTTTTCTTACCAAACTTTCTTCTTCAAAACTTTCTTCTTCAATCCGGGCAATGGGCTCTTCAGCTTCTTCTCACTCCAACGACGCAAACACTACAGAAATTCAATCAGGTATTCATTATTCTCTTGGGTTTTCCTCACTTTTTTTTTTCTTTACTGAAATCAATTTAGTTTAGTATATAACAAGCTATATTTCGATGTCAAAAGTATCTAGTTTTGCTCATAGACTATTTCAACATATGATTCAAACATAACTTAATACTTAATATTTTGATAGAAACAAACTGGTATCAAGATTGATAAGTTTAGAAGGGAAAAAAAATCTAGTGGGTCGAACAATTTTTTGTCAGACTATACTTACTTAATGGTCGAACTTAGGATTACTAGAGCCATTTCCTTGGGAACTGGAGGATTAACACTTAACAGCCGAAAAAATATTATTAGGAGGGGGGCCATTAATAACTCTGTTTGTAGGGGTATGGGGGGCTGTGATGTTGAGGAAGGTTTTGATTAGATGTTCTTTGAATGCCCATTGTTTTCAAGGTTGTGCGAGGTGGTAGTGGGTTGCTTGGGTATGACTACTGCTCTTACAAATTCAGAAATTTATTATTCAATTTGCTGGCTTTGTGGAGTGGAGGAAGAGTAATTGAACAAAATTGTACAAGAAAATGGCGAGGGAAGTGTTGAATTTATATTAATAACTGAAGTGAAAATGAAAATAGGAGAAATTGGAATTAAACAAACAGCCCTTAATGTTGTGTCTGAAGAGTTCGAGTAAGAAGGTTTTGCCGTGTAGTGTGTATTCGCAGGGTGGGCAGCAGGAAAGATGAAGACACTTTGCAGCAGATGGCGTTGATGTTTCTCTCGAAAACAATGATATCCCAAATCTGCTGCAGAAGTGTGGGAAGCTATCCCAAACTATGGCTTCACATGAGATGGTGAAGGGGCTTAATTGGAGTGGTAAATTGTTTTGGCGGATGGTTTTGGAGGGTTGCTAAGCTTAAGTCATCACCTGGATCTGTGCTGATTAGTGCACCTCTTAAAGGCTTGATGTTGAGCACGTAGCTTAACCATCATGAACCTGAAAAGTTGTATGTTGGTTATAAGGCTGATGGAGATACATATAGCTAAATTTTATGTAAAAGTGTGTTCCCGTCGTAAGACTGGAGTGGAGTTAATAGGTGTGGGGTGTGATGTAATGCTTGCTGTTGAAAATTGAATGGGGAGTAGAAGTATTAAAACCAACATACATGTAACGTTTAGAGGGGTGGTGCTAGTTTTTATTTTAGACGTCTCTATGCTATTTTCAGTGATGGTAGTAGCAAGGTGTATGACGTGTTTGTAGTCCTTAGCATCTCTTGTTCTTGGTCTAGTGTGTTGTCAATCACTTACTTTTTGTGTGTATTATTGAAATGTGCGTAAATTGTAGAAATAAAAGTAATAAAGAGATTGATATATAGGAACGGTGTCTGTATTTTGACATTCAAATAATGCGATCGATATATATGTGCTTTGGTCCTCCAATCCAACAACTTCTTTCTGTGCTTTGCAGGATCTAGTCCTGTTGATTATAAATCTTTAAGTGATGCAGAATGGAAGAAGCAGCTAACCGATGAACAATTTTATGTTACTCGGAAAAAGGGAACCGAGAGAGCTTTCACTGGGTGAGTTTAAGATTGGGCTTAATAGTCGTGCATGTCATTTGCAATTTCTTTTTCAAGATTCGTTCAATGCTATGTAGCAGCAACATTTCTTTGAGTGTCTAACACATGTTGTCTGACACTCATACGACACGGACACATACTTTCAATCGTTTTTATTTCCTCAAATTATTACGGACCTTGGTATTATGTCCGTTATCTGTGTCTGTGTTGGTGCCAAAGTCGTGTTACCATGGCCTTTTGACTTGCTTATCACTCAGCTACTAACTTTCCTTGATTTTTATGTCTATTTAATTGAAATTTATCGATTATGTTAATTCAGTTCACCAAATTTATTTTACATTAAAGGAAATATCTCAACACACAATCATTGAAAGAAGGAAGTTTGAATGCACCTCATTGATTGCAGTTTTGTTCACAAATGTGGCATACTCAGTAAAGTAGCTACTTCTGTTTTATAACAGTTTTCTAGTTCTCACCATTGGCCAATGAACATCACACAGGTTTCTTATATCATATTATTCCACAATTAGTTGAAGATGTCACATTCAAAAGTTGTTCATTGTATTTCACTTGTGGTTAATATTAGTAGTACCTGAAATGAAAGATACAAGGATTTCAGTTGCATAAAGTATTGTATAAAAGTCTTGTGATGTTCTGAGTGTTTCACATTATTGAAAAGAAATCCAAGTTCTATATTTAAAACGGGAAAGGTCTTGAAAAGAGTTTATAAAGAGAGACACCTGTCGCCTTTCAAGTCAATTTTGTAAGGACGAGTTAAACCCAATATGAAAAACTTGATACACAATGCTGAGTTAGTTTTGTGTGCATTTGGCTTTAATACTTGGTGTCCATATATGATTAAGGGCAATGTAAGGAAGCATACCATATATTCTTGTGACAAAGTTTACTTCTCTACCATGTTGGTTCTCTAAAGAGATATTGTGGTCTGTGCTATACTATTTATTGTATTTAACTGTTTTTTTAATTCATTTTAGGGAGTACTGGAATACCAAAACTGAAGGGACTTACCATTGCATATGTTGTGACACACCCTTATTTGAGTAAGTACTTTATGCATGTATGTATAGTACACCTGTTCGCTTGCGCATACTCACATGTATGTATTTATTTAACCAAATATATGGCCTGAATCATCTTGAACTTATAAACTTCTCTTGTGTTTTCCAGATCATCTACCAAGTTTAATAGTGGAACTGGTTGGCCATCTTATTACCAGCCAATTGGTAAAAATGTGAAATCAAAGCTAGATTTGTCAATTATATTCATGCCCCGCCAAGAAGTTCTCTGTGCTGTTTGTGATGCTCATCTCGGACACATTTTTGATGATGGCCCTCCACCCACAGGAAAACGTTACTGTATCAACAGGTAAAGAATCACTTGTTGAATTGGGTTTCTTTTGCACCTGTATAAGCTCACATTTGTCTAAAAGCTTGTTTGTTTTGGAATTTCTGCAGTGCTTCCCTTAAGCTCAAACCAAGGAAGTAA

Protein sequence

>MS.gene32271.t1

MASHSLSLSAAAQILPNTQIHKFDSKLLLWPSRFLTKLSSSKLSSSIRAMGSSASSHSNDANTTEIQSGSSPVDYKSLSDAEWKKQLTDEQFYVTRKKGTERAFTGEYWNTKTEGTYHCICCDTPLFESSTKFNSGTGWPSYYQPIGKNVKSKLDLSIIFMPRQEVLCAVCDAHLGHIFDDGPPPTGKRYCINSASLKLKPRK