AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34225


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34225.t1 MTR_8g061130 97.279 147 3 1 1 146 1 147 5.74e-101 286
MS.gene34225.t1 MTR_0002s0770 73.529 68 18 0 70 137 15 82 1.74e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34225.t1 AT4G11175 69.014 71 22 0 67 137 67 137 1.01e-30 108

Find 73 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATGGTGGGGTGGGGTTT+AGG 0.140970 8.4:+47675625 None:intergenic
GGATGGTGGGGTGGGGTTTA+GGG 0.154902 8.4:+47675626 None:intergenic
CCAAAGGACGTATCATTTAT+AGG 0.219493 8.4:-47675380 MS.gene34225:CDS
TTTGTCAGGGGTTGAGGATT+TGG 0.259095 8.4:+47675587 None:intergenic
TTCTGCTCGCTGGCTTTGTC+AGG 0.284276 8.4:+47675573 None:intergenic
GATGGTGGGGTGGGGTTTAG+GGG 0.321440 8.4:+47675627 None:intergenic
ACAAAGCCAGCGAGCAGAAA+TGG 0.346149 8.4:-47675569 MS.gene34225:CDS
GGTGAAGATGGATGGTGGGG+TGG 0.347822 8.4:+47675617 None:intergenic
TGGTGGGGTGGGGTTTAGGG+GGG 0.353477 8.4:+47675629 None:intergenic
GTTGGCGATGGTGAATGATT+GGG 0.370868 8.4:+47675733 None:intergenic
GATGGAGGGCGGAGGAAGGA+TGG 0.371912 8.4:+47675651 None:intergenic
TGGGGTGCGAAAGTGGAGAG+TGG 0.374140 8.4:+47675671 None:intergenic
GAATGATTGGGGCGTGGAAT+GGG 0.377126 8.4:+47675745 None:intergenic
ATGGTGGGGTGGGGTTTAGG+GGG 0.383430 8.4:+47675628 None:intergenic
AATTACGTTCGGATCCTTCC+TGG 0.385097 8.4:-47675441 MS.gene34225:CDS
GCTGGCTTTGTCAGGGGTTG+AGG 0.393571 8.4:+47675581 None:intergenic
TGAAGATGGATGGTGGGGTG+GGG 0.398425 8.4:+47675619 None:intergenic
ATGGAGGGCGGAGGAAGGAT+GGG 0.406506 8.4:+47675652 None:intergenic
GTGAAGATGGATGGTGGGGT+GGG 0.408157 8.4:+47675618 None:intergenic
GGTTGGCGATGGTGAATGAT+TGG 0.410559 8.4:+47675732 None:intergenic
CCTATAAATGATACGTCCTT+TGG 0.416877 8.4:+47675380 None:intergenic
CGAACACGGAACATGCCGTT+AGG 0.422494 8.4:+47675516 None:intergenic
GGATCCGAACGTAATTCTTT+CGG 0.427721 8.4:+47675448 None:intergenic
CTTATCCTTGGATATATCTC+TGG 0.434923 8.4:-47675477 MS.gene34225:CDS
TGGAGGGCGGAGGAAGGATG+GGG 0.435398 8.4:+47675653 None:intergenic
GTGGACCCATTTCTGCTCGC+TGG 0.449738 8.4:+47675563 None:intergenic
GGAAGGATGGGGTGCGAAAG+TGG 0.455893 8.4:+47675664 None:intergenic
TGAATGATTGGGGCGTGGAA+TGG 0.456507 8.4:+47675744 None:intergenic
TCTGCTCGCTGGCTTTGTCA+GGG 0.460798 8.4:+47675574 None:intergenic
GGGGTGCGAAAGTGGAGAGT+GGG 0.461179 8.4:+47675672 None:intergenic
GAGTGGGTGGTGACGGTAGA+GGG 0.464511 8.4:+47675711 None:intergenic
CAGAGATATATCCAAGGATA+AGG 0.465359 8.4:+47675478 None:intergenic
GGGTGCGAAAGTGGAGAGTG+GGG 0.467038 8.4:+47675673 None:intergenic
GTCAGGGGTTGAGGATTTGG+CGG 0.477583 8.4:+47675590 None:intergenic
CAAAGCCAGCGAGCAGAAAT+GGG 0.481491 8.4:-47675568 MS.gene34225:CDS
GGTTGAGGATTTGGCGGCGA+CGG 0.484284 8.4:+47675596 None:intergenic
GATAATGAAGACCTTATCCT+TGG 0.484697 8.4:-47675489 MS.gene34225:CDS
TATAGGCTTCGTAATACTCC+TGG 0.487758 8.4:-47675363 MS.gene34225:CDS
TTTGGCGGCGACGGTGAAGA+TGG 0.493162 8.4:+47675605 None:intergenic
GATTGGGGCGTGGAATGGGA+TGG 0.497058 8.4:+47675749 None:intergenic
GGAGTGGGTGGTGACGGTAG+AGG 0.509085 8.4:+47675710 None:intergenic
CGACGGTGAAGATGGATGGT+GGG 0.513263 8.4:+47675613 None:intergenic
ATAAATGATACGTCCTTTGG+AGG 0.516040 8.4:+47675383 None:intergenic
GCGACGGTGAAGATGGATGG+TGG 0.519916 8.4:+47675612 None:intergenic
AGAAAAGGAGTGGGTGGTGA+CGG 0.521019 8.4:+47675704 None:intergenic
AACTAAGAAGAGCCACCACC+AGG 0.533810 8.4:+47675345 None:intergenic
CTGCTCGCTGGCTTTGTCAG+GGG 0.538260 8.4:+47675575 None:intergenic
CTTCGTAATACTCCTGGTGG+TGG 0.553297 8.4:-47675357 MS.gene34225:CDS
GGGTGGTGACGGTAGAGGGT+TGG 0.556218 8.4:+47675715 None:intergenic
TTGGCGATGGTGAATGATTG+GGG 0.557416 8.4:+47675734 None:intergenic
GTGGAATGGGATGGAAGATG+AGG 0.567978 8.4:+47675758 None:intergenic
GCGGCGACGGTGAAGATGGA+TGG 0.571559 8.4:+47675609 None:intergenic
GGATCCGAAAGAATTACGTT+CGG 0.574169 8.4:-47675452 MS.gene34225:CDS
CACGGAACATGCCGTTAGGG+AGG 0.578464 8.4:+47675520 None:intergenic
GAACACGGAACATGCCGTTA+GGG 0.578793 8.4:+47675517 None:intergenic
AGTCGATATGATTCCTCCAA+AGG 0.579977 8.4:-47675396 MS.gene34225:CDS
CTTCATTATCGAGACGAACA+CGG 0.580715 8.4:+47675502 None:intergenic
TCAACTCTAACTCTATCTCC+AGG 0.592855 8.4:+47675423 None:intergenic
ATCACAGAATCCCTCCCTAA+CGG 0.596048 8.4:-47675531 MS.gene34225:CDS
GACGGTGAAGATGGATGGTG+GGG 0.596186 8.4:+47675614 None:intergenic
GGTGCGAAAGTGGAGAGTGG+GGG 0.609773 8.4:+47675674 None:intergenic
TGACGGTAGAGGGTTGGCGA+TGG 0.612664 8.4:+47675721 None:intergenic
CTCTAACTCTATCTCCAGGA+AGG 0.615980 8.4:+47675427 None:intergenic
GGGTGTGGAAAGAAAAGGAG+TGG 0.621177 8.4:+47675694 None:intergenic
GTGGAAAGAAAAGGAGTGGG+TGG 0.622441 8.4:+47675698 None:intergenic
ATTCTGTGATGAGACCTTCG+TGG 0.627359 8.4:+47675544 None:intergenic
ACGGAACATGCCGTTAGGGA+GGG 0.631389 8.4:+47675521 None:intergenic
GATGGTGAATGATTGGGGCG+TGG 0.638062 8.4:+47675739 None:intergenic
TCCTTGGATATATCTCTGGA+AGG 0.641053 8.4:-47675473 MS.gene34225:CDS
TCCTTCCAGAGATATATCCA+AGG 0.654970 8.4:+47675472 None:intergenic
GGTGTGGAAAGAAAAGGAGT+GGG 0.666040 8.4:+47675695 None:intergenic
AGGCTTCGTAATACTCCTGG+TGG 0.702905 8.4:-47675360 MS.gene34225:CDS
GAGCAGAAATGGGTCCACGA+AGG 0.710770 8.4:-47675558 MS.gene34225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAAATGATACGTCCTTTGG+AGG + chr8.4:47675732-47675751 None:intergenic 35.0%
CAGAGATATATCCAAGGATA+AGG + chr8.4:47675637-47675656 None:intergenic 35.0%
CCAAAGGACGTATCATTTAT+AGG - chr8.4:47675732-47675751 MS.gene34225:CDS 35.0%
CCTATAAATGATACGTCCTT+TGG + chr8.4:47675735-47675754 None:intergenic 35.0%
CTTATCCTTGGATATATCTC+TGG - chr8.4:47675635-47675654 MS.gene34225:CDS 35.0%
GATAATGAAGACCTTATCCT+TGG - chr8.4:47675623-47675642 MS.gene34225:CDS 35.0%
AGTCGATATGATTCCTCCAA+AGG - chr8.4:47675716-47675735 MS.gene34225:CDS 40.0%
CTTCATTATCGAGACGAACA+CGG + chr8.4:47675613-47675632 None:intergenic 40.0%
GGATCCGAAAGAATTACGTT+CGG - chr8.4:47675660-47675679 MS.gene34225:CDS 40.0%
GGATCCGAACGTAATTCTTT+CGG + chr8.4:47675667-47675686 None:intergenic 40.0%
TATAGGCTTCGTAATACTCC+TGG - chr8.4:47675749-47675768 MS.gene34225:CDS 40.0%
TCAACTCTAACTCTATCTCC+AGG + chr8.4:47675692-47675711 None:intergenic 40.0%
TCCTTCCAGAGATATATCCA+AGG + chr8.4:47675643-47675662 None:intergenic 40.0%
TCCTTGGATATATCTCTGGA+AGG - chr8.4:47675639-47675658 MS.gene34225:CDS 40.0%
AATTACGTTCGGATCCTTCC+TGG - chr8.4:47675671-47675690 MS.gene34225:CDS 45.0%
ATCACAGAATCCCTCCCTAA+CGG - chr8.4:47675581-47675600 MS.gene34225:CDS 45.0%
ATTCTGTGATGAGACCTTCG+TGG + chr8.4:47675571-47675590 None:intergenic 45.0%
CTCTAACTCTATCTCCAGGA+AGG + chr8.4:47675688-47675707 None:intergenic 45.0%
GGTGTGGAAAGAAAAGGAGT+GGG + chr8.4:47675420-47675439 None:intergenic 45.0%
GTTGGCGATGGTGAATGATT+GGG + chr8.4:47675382-47675401 None:intergenic 45.0%
TTGGCGATGGTGAATGATTG+GGG + chr8.4:47675381-47675400 None:intergenic 45.0%
TTTGTCAGGGGTTGAGGATT+TGG + chr8.4:47675528-47675547 None:intergenic 45.0%
ACAAAGCCAGCGAGCAGAAA+TGG - chr8.4:47675543-47675562 MS.gene34225:CDS 50.0%
AGAAAAGGAGTGGGTGGTGA+CGG + chr8.4:47675411-47675430 None:intergenic 50.0%
AGGCTTCGTAATACTCCTGG+TGG - chr8.4:47675752-47675771 MS.gene34225:CDS 50.0%
CAAAGCCAGCGAGCAGAAAT+GGG - chr8.4:47675544-47675563 MS.gene34225:CDS 50.0%
CTTCGTAATACTCCTGGTGG+TGG - chr8.4:47675755-47675774 MS.gene34225:CDS 50.0%
GAACACGGAACATGCCGTTA+GGG + chr8.4:47675598-47675617 None:intergenic 50.0%
GAATGATTGGGGCGTGGAAT+GGG + chr8.4:47675370-47675389 None:intergenic 50.0%
GGGTGTGGAAAGAAAAGGAG+TGG + chr8.4:47675421-47675440 None:intergenic 50.0%
GGTTGGCGATGGTGAATGAT+TGG + chr8.4:47675383-47675402 None:intergenic 50.0%
GTGGAAAGAAAAGGAGTGGG+TGG + chr8.4:47675417-47675436 None:intergenic 50.0%
GTGGAATGGGATGGAAGATG+AGG + chr8.4:47675357-47675376 None:intergenic 50.0%
TGAATGATTGGGGCGTGGAA+TGG + chr8.4:47675371-47675390 None:intergenic 50.0%
! AGTGGGGGTGTGGAAAGAAA+AGG + chr8.4:47675426-47675445 None:intergenic 50.0%
ACGGAACATGCCGTTAGGGA+GGG + chr8.4:47675594-47675613 None:intergenic 55.0%
CGAACACGGAACATGCCGTT+AGG + chr8.4:47675599-47675618 None:intergenic 55.0%
CGACGGTGAAGATGGATGGT+GGG + chr8.4:47675502-47675521 None:intergenic 55.0%
GACGGTGAAGATGGATGGTG+GGG + chr8.4:47675501-47675520 None:intergenic 55.0%
GAGCAGAAATGGGTCCACGA+AGG - chr8.4:47675554-47675573 MS.gene34225:CDS 55.0%
GATGGTGAATGATTGGGGCG+TGG + chr8.4:47675376-47675395 None:intergenic 55.0%
GTCAGGGGTTGAGGATTTGG+CGG + chr8.4:47675525-47675544 None:intergenic 55.0%
!! GTGAAGATGGATGGTGGGGT+GGG + chr8.4:47675497-47675516 None:intergenic 55.0%
!! TCTGCTCGCTGGCTTTGTCA+GGG + chr8.4:47675541-47675560 None:intergenic 55.0%
!! TGAAGATGGATGGTGGGGTG+GGG + chr8.4:47675496-47675515 None:intergenic 55.0%
!! TTCTGCTCGCTGGCTTTGTC+AGG + chr8.4:47675542-47675561 None:intergenic 55.0%
ATGGAGGGCGGAGGAAGGAT+GGG + chr8.4:47675463-47675482 None:intergenic 60.0%
CACGGAACATGCCGTTAGGG+AGG + chr8.4:47675595-47675614 None:intergenic 60.0%
GAAAGTGGAGAGTGGGGGTG+TGG + chr8.4:47675436-47675455 None:intergenic 60.0%
GATTGGGGCGTGGAATGGGA+TGG + chr8.4:47675366-47675385 None:intergenic 60.0%
GCGACGGTGAAGATGGATGG+TGG + chr8.4:47675503-47675522 None:intergenic 60.0%
GGAAGGATGGGGTGCGAAAG+TGG + chr8.4:47675451-47675470 None:intergenic 60.0%
GGGGTGCGAAAGTGGAGAGT+GGG + chr8.4:47675443-47675462 None:intergenic 60.0%
GGGTGCGAAAGTGGAGAGTG+GGG + chr8.4:47675442-47675461 None:intergenic 60.0%
GGTGCGAAAGTGGAGAGTGG+GGG + chr8.4:47675441-47675460 None:intergenic 60.0%
GGTTGAGGATTTGGCGGCGA+CGG + chr8.4:47675519-47675538 None:intergenic 60.0%
GTGGACCCATTTCTGCTCGC+TGG + chr8.4:47675552-47675571 None:intergenic 60.0%
TGGGGTGCGAAAGTGGAGAG+TGG + chr8.4:47675444-47675463 None:intergenic 60.0%
TGGGGTTTAGGGGGGATGGA+GGG + chr8.4:47675478-47675497 None:intergenic 60.0%
TTTGGCGGCGACGGTGAAGA+TGG + chr8.4:47675510-47675529 None:intergenic 60.0%
! ATGGTGGGGTGGGGTTTAGG+GGG + chr8.4:47675487-47675506 None:intergenic 60.0%
! TGACGGTAGAGGGTTGGCGA+TGG + chr8.4:47675394-47675413 None:intergenic 60.0%
!! CTGCTCGCTGGCTTTGTCAG+GGG + chr8.4:47675540-47675559 None:intergenic 60.0%
!! GAGTGGGTGGTGACGGTAGA+GGG + chr8.4:47675404-47675423 None:intergenic 60.0%
!! GATGGTGGGGTGGGGTTTAG+GGG + chr8.4:47675488-47675507 None:intergenic 60.0%
!! GCTGGCTTTGTCAGGGGTTG+AGG + chr8.4:47675534-47675553 None:intergenic 60.0%
!! GGATGGTGGGGTGGGGTTTA+GGG + chr8.4:47675489-47675508 None:intergenic 60.0%
!! GGTGAAGATGGATGGTGGGG+TGG + chr8.4:47675498-47675517 None:intergenic 60.0%
!! TGGATGGTGGGGTGGGGTTT+AGG + chr8.4:47675490-47675509 None:intergenic 60.0%
GATGGAGGGCGGAGGAAGGA+TGG + chr8.4:47675464-47675483 None:intergenic 65.0%
GCGGCGACGGTGAAGATGGA+TGG + chr8.4:47675506-47675525 None:intergenic 65.0%
GGTTTAGGGGGGATGGAGGG+CGG + chr8.4:47675475-47675494 None:intergenic 65.0%
GTGGGGTTTAGGGGGGATGG+AGG + chr8.4:47675479-47675498 None:intergenic 65.0%
TGGAGGGCGGAGGAAGGATG+GGG + chr8.4:47675462-47675481 None:intergenic 65.0%
TGGTGGGGTGGGGTTTAGGG+GGG + chr8.4:47675486-47675505 None:intergenic 65.0%
!! GGAGTGGGTGGTGACGGTAG+AGG + chr8.4:47675405-47675424 None:intergenic 65.0%
!! GGGTGGTGACGGTAGAGGGT+TGG + chr8.4:47675400-47675419 None:intergenic 65.0%
GGGGTGGGGTTTAGGGGGGA+TGG + chr8.4:47675482-47675501 None:intergenic 70.0%
TTAGGGGGGATGGAGGGCGG+AGG + chr8.4:47675472-47675491 None:intergenic 70.0%
GGGGGATGGAGGGCGGAGGA+AGG + chr8.4:47675468-47675487 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 47675347 47675787 47675347 ID=MS.gene34225
chr8.4 mRNA 47675347 47675787 47675347 ID=MS.gene34225.t1;Parent=MS.gene34225
chr8.4 exon 47675347 47675787 47675347 ID=MS.gene34225.t1.exon1;Parent=MS.gene34225.t1
chr8.4 CDS 47675347 47675787 47675347 ID=cds.MS.gene34225.t1;Parent=MS.gene34225.t1
Gene Sequence

>MS.gene34225

ATGTTCACCTCATCTTCCATCCCATTCCACGCCCCAATCATTCACCATCGCCAACCCTCTACCGTCACCACCCACTCCTTTTCTTTCCACACCCCCACTCTCCACTTTCGCACCCCATCCTTCCTCCGCCCTCCATCCCCCCTAAACCCCACCCCACCATCCATCTTCACCGTCGCCGCCAAATCCTCAACCCCTGACAAAGCCAGCGAGCAGAAATGGGTCCACGAAGGTCTCATCACAGAATCCCTCCCTAACGGCATGTTCCGTGTTCGTCTCGATAATGAAGACCTTATCCTTGGATATATCTCTGGAAGGATCCGAAAGAATTACGTTCGGATCCTTCCTGGAGATAGAGTTAGAGTTGAAGTTAGTCGATATGATTCCTCCAAAGGACGTATCATTTATAGGCTTCGTAATACTCCTGGTGGTGGCTCTTCTTAG

Protein sequence

>MS.gene34225.t1

MFTSSSIPFHAPIIHHRQPSTVTTHSFSFHTPTLHFRTPSFLRPPSPLNPTPPSIFTVAAKSSTPDKASEQKWVHEGLITESLPNGMFRVRLDNEDLILGYISGRIRKNYVRILPGDRVRVEVSRYDSSKGRIIYRLRNTPGGGSS