AlfalfaGEDB | MS.gene35808

M. sativa cultivar XinJiangDaYe / MS.gene35808

KEGG: K05387
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35808.t1	KEH39010.1	100	64	0	0	1	64	1	64	9.30E-26	125.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35808.t1	A0A072VLI4	100.0	64	0	0	1	64	1	64	6.7e-26	125.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene35808	0.837514	4.97E-57	-1.69E-46
MS.gene049159	MS.gene35808	0.859627	3.63E-63	-1.69E-46
MS.gene049368	MS.gene35808	0.811177	7.97E-51	-1.69E-46
MS.gene049435	MS.gene35808	0.823016	1.74E-53	-1.69E-46
MS.gene049585	MS.gene35808	0.820011	8.59E-53	-1.69E-46
MS.gene049647	MS.gene35808	0.828368	9.35E-55	-1.69E-46
MS.gene049801	MS.gene35808	0.804981	1.66E-49	-1.69E-46
MS.gene049983	MS.gene35808	0.811102	8.28E-51	-1.69E-46
MS.gene050105	MS.gene35808	0.822783	1.97E-53	-1.69E-46
MS.gene050361	MS.gene35808	0.836848	7.36E-57	-1.69E-46
MS.gene050442	MS.gene35808	0.823014	1.74E-53	-1.69E-46
MS.gene050654	MS.gene35808	0.808419	3.13E-50	-1.69E-46
MS.gene050680	MS.gene35808	0.826987	2.01E-54	-1.69E-46
MS.gene050762	MS.gene35808	0.82521	5.31E-54	-1.69E-46
MS.gene050763	MS.gene35808	0.825022	5.88E-54	-1.69E-46
MS.gene050780	MS.gene35808	0.802324	5.92E-49	-1.69E-46
MS.gene050807	MS.gene35808	0.852403	4.72E-61	-1.69E-46
MS.gene050808	MS.gene35808	0.814133	1.80E-51	-1.69E-46
MS.gene050826	MS.gene35808	0.85323	2.74E-61	-1.69E-46
MS.gene050941	MS.gene35808	0.806737	7.11E-50	-1.69E-46
MS.gene051066	MS.gene35808	0.826319	2.90E-54	-1.69E-46
MS.gene051180	MS.gene35808	0.838848	2.25E-57	-1.69E-46
MS.gene051198	MS.gene35808	0.827917	1.20E-54	-1.69E-46
MS.gene051452	MS.gene35808	0.806618	7.54E-50	-1.69E-46
MS.gene051800	MS.gene35808	0.839007	2.05E-57	-1.69E-46
MS.gene051856	MS.gene35808	0.812751	3.62E-51	-1.69E-46
MS.gene052012	MS.gene35808	0.816029	6.82E-52	-1.69E-46
MS.gene052426	MS.gene35808	0.821099	4.83E-53	-1.69E-46
MS.gene052516	MS.gene35808	0.803847	2.87E-49	-1.69E-46
MS.gene052674	MS.gene35808	0.832954	7.04E-56	-1.69E-46
MS.gene052675	MS.gene35808	0.839345	1.68E-57	-1.69E-46
MS.gene052677	MS.gene35808	0.84308	1.75E-58	-1.69E-46
MS.gene052863	MS.gene35808	0.810315	1.23E-50	-1.69E-46
MS.gene053008	MS.gene35808	0.844578	6.93E-59	-1.69E-46
MS.gene053166	MS.gene35808	0.838625	2.57E-57	-1.69E-46
MS.gene05350	MS.gene35808	0.802138	6.47E-49	-1.69E-46
MS.gene054139	MS.gene35808	0.801686	8.01E-49	-1.69E-46
MS.gene05415	MS.gene35808	0.801253	9.82E-49	-1.69E-46
MS.gene054236	MS.gene35808	0.821566	3.77E-53	-1.69E-46
MS.gene054379	MS.gene35808	0.842978	1.86E-58	-1.69E-46
MS.gene054467	MS.gene35808	0.803483	3.41E-49	-1.69E-46
MS.gene054472	MS.gene35808	0.821523	3.86E-53	-1.69E-46
MS.gene054479	MS.gene35808	0.829132	6.11E-55	-1.69E-46
MS.gene054559	MS.gene35808	0.80106	1.08E-48	-1.69E-46
MS.gene05458	MS.gene35808	0.810678	1.02E-50	-1.69E-46
MS.gene054657	MS.gene35808	0.819091	1.39E-52	-1.69E-46
MS.gene05468	MS.gene35808	0.818965	1.49E-52	-1.69E-46
MS.gene054775	MS.gene35808	0.819716	1.00E-52	-1.69E-46
MS.gene05510	MS.gene35808	0.846247	2.45E-59	-1.69E-46
MS.gene055227	MS.gene35808	0.831995	1.22E-55	-1.69E-46
MS.gene055228	MS.gene35808	0.821358	4.21E-53	-1.69E-46
MS.gene055230	MS.gene35808	0.80657	7.72E-50	-1.69E-46
MS.gene055310	MS.gene35808	0.828999	6.58E-55	-1.69E-46
MS.gene055311	MS.gene35808	0.815095	1.10E-51	-1.69E-46
MS.gene055313	MS.gene35808	0.822866	1.88E-53	-1.69E-46
MS.gene055608	MS.gene35808	0.819897	9.13E-53	-1.69E-46
MS.gene055623	MS.gene35808	0.815709	8.05E-52	-1.69E-46
MS.gene055701	MS.gene35808	0.863581	2.25E-64	-1.69E-46
MS.gene055818	MS.gene35808	0.820295	7.40E-53	-1.69E-46
MS.gene055926	MS.gene35808	0.878368	2.97E-69	-1.69E-46
MS.gene056045	MS.gene35808	0.812773	3.58E-51	-1.69E-46
MS.gene05604	MS.gene35808	0.819551	1.09E-52	-1.69E-46
MS.gene056181	MS.gene35808	0.818318	2.09E-52	-1.69E-46
MS.gene056480	MS.gene35808	0.835811	1.35E-56	-1.69E-46
MS.gene056552	MS.gene35808	0.802671	5.02E-49	-1.69E-46
MS.gene057394	MS.gene35808	0.831637	1.49E-55	-1.69E-46
MS.gene057444	MS.gene35808	0.80162	8.26E-49	-1.69E-46
MS.gene05761	MS.gene35808	0.804466	2.13E-49	-1.69E-46
MS.gene057693	MS.gene35808	0.814333	1.63E-51	-1.69E-46
MS.gene057855	MS.gene35808	0.813879	2.05E-51	-1.69E-46
MS.gene057869	MS.gene35808	0.819194	1.32E-52	-1.69E-46
MS.gene057915	MS.gene35808	0.809033	2.31E-50	-1.69E-46
MS.gene058010	MS.gene35808	0.838565	2.67E-57	-1.69E-46
MS.gene058081	MS.gene35808	0.833799	4.33E-56	-1.69E-46
MS.gene058146	MS.gene35808	0.82763	1.41E-54	-1.69E-46
MS.gene058223	MS.gene35808	-0.80431	2.30E-49	-1.69E-46
MS.gene058292	MS.gene35808	0.806389	8.42E-50	-1.69E-46
MS.gene058339	MS.gene35808	0.802554	5.31E-49	-1.69E-46
MS.gene05847	MS.gene35808	0.819685	1.02E-52	-1.69E-46
MS.gene058595	MS.gene35808	0.840667	7.58E-58	-1.69E-46
MS.gene058647	MS.gene35808	0.822751	2.00E-53	-1.69E-46
MS.gene058979	MS.gene35808	0.82786	1.24E-54	-1.69E-46
MS.gene05897	MS.gene35808	0.814675	1.37E-51	-1.69E-46
MS.gene059128	MS.gene35808	0.818534	1.86E-52	-1.69E-46
MS.gene059161	MS.gene35808	0.815774	7.78E-52	-1.69E-46
MS.gene059283	MS.gene35808	0.813146	2.97E-51	-1.69E-46
MS.gene059439	MS.gene35808	0.851388	9.17E-61	-1.69E-46
MS.gene059561	MS.gene35808	0.833116	6.42E-56	-1.69E-46
MS.gene059713	MS.gene35808	0.814585	1.43E-51	-1.69E-46
MS.gene059725	MS.gene35808	0.818568	1.83E-52	-1.69E-46
MS.gene05973	MS.gene35808	0.813733	2.20E-51	-1.69E-46
MS.gene059842	MS.gene35808	0.825452	4.65E-54	-1.69E-46
MS.gene059952	MS.gene35808	0.81085	9.39E-51	-1.69E-46
MS.gene060011	MS.gene35808	0.821829	3.28E-53	-1.69E-46
MS.gene06010	MS.gene35808	0.803931	2.75E-49	-1.69E-46
MS.gene060167	MS.gene35808	0.800654	1.30E-48	-1.69E-46
MS.gene060296	MS.gene35808	0.831606	1.52E-55	-1.69E-46
MS.gene060310	MS.gene35808	0.83107	2.06E-55	-1.69E-46
MS.gene060322	MS.gene35808	0.837721	4.40E-57	-1.69E-46
MS.gene06052	MS.gene35808	0.826998	1.99E-54	-1.69E-46
MS.gene060637	MS.gene35808	0.804919	1.71E-49	-1.69E-46
MS.gene060683	MS.gene35808	0.838292	3.14E-57	-1.69E-46
MS.gene060685	MS.gene35808	0.844848	5.86E-59	-1.69E-46
MS.gene060802	MS.gene35808	0.859671	3.52E-63	-1.69E-46
MS.gene060803	MS.gene35808	0.835425	1.69E-56	-1.69E-46
MS.gene060870	MS.gene35808	0.811311	7.46E-51	-1.69E-46
MS.gene060932	MS.gene35808	0.803925	2.76E-49	-1.69E-46
MS.gene060935	MS.gene35808	0.809024	2.32E-50	-1.69E-46
MS.gene060998	MS.gene35808	0.851539	8.31E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35808.t1	MTR_2g088450	100.000	64	1	64	1	64	1.74e-36	128
MS.gene35808.t1	MTR_2g088450	100.000	64	1	64	1	64	1.79e-36	128
MS.gene35808.t1	MTR_2g088450	100.000	64	1	64	1	64	2.48e-36	128

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 10 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAATTGCAGTGGATTTAT+TGG	0.178126	2.4:-12587588	MS.gene35808:intron
AGACCAACTGTTGTGAAAAT+TGG	0.195940	2.4:-12587738	MS.gene35808:CDS
AATGAAGTTGTGGCTATAAT+TGG	0.217187	2.4:-12587780	MS.gene35808:CDS
ATTATAGCCACAACTTCATT+TGG	0.318223	2.4:+12587783	None:intergenic
ATGGAGGTGTTAGGAGTAAA+GGG	0.377419	2.4:-12587837	MS.gene35808:CDS
TTTACTGTTGATTCTGTGAT+TGG	0.383641	2.4:-12587708	MS.gene35808:CDS
GGATTTATTGGAACAGTTGA+AGG	0.435584	2.4:-12587576	None:intergenic
AATGGAGGTGTTAGGAGTAA+AGG	0.453081	2.4:-12587838	MS.gene35808:CDS
GTGGATTCCAAATGAAGTTG+TGG	0.579560	2.4:-12587790	MS.gene35808:CDS
ATTGGAAGATCAGCTCAACA+AGG	0.681994	2.4:-12587690	MS.gene35808:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATTCTTCCTGGTATTAAAA+TGG	-	chr2.4:12587788-12587807	MS.gene35808:CDS	25.0%
!!!	ATAACATCCATTTTAATACC+AGG	+	chr2.4:12587798-12587817	None:intergenic	25.0%
	AATGAAGTTGTGGCTATAAT+TGG	-	chr2.4:12587636-12587655	MS.gene35808:intron	30.0%
	ATTATAGCCACAACTTCATT+TGG	+	chr2.4:12587636-12587655	None:intergenic	30.0%
	TTTCATGATACAAATTGCAG+TGG	-	chr2.4:12587819-12587838	MS.gene35808:CDS	30.0%
!	ACAAATTGCAGTGGATTTAT+TGG	-	chr2.4:12587828-12587847	MS.gene35808:CDS	30.0%
!!	TTTACTGTTGATTCTGTGAT+TGG	-	chr2.4:12587708-12587727	MS.gene35808:CDS	30.0%
	AGACCAACTGTTGTGAAAAT+TGG	-	chr2.4:12587678-12587697	MS.gene35808:CDS	35.0%
	GCTAATAAGACTATTCTTCC+TGG	-	chr2.4:12587777-12587796	MS.gene35808:CDS	35.0%
	TCCACAAACAGAAAAACAAG+AGG	+	chr2.4:12587611-12587630	None:intergenic	35.0%
!!!	TCCTCTTGTTTTTCTGTTTG+TGG	-	chr2.4:12587607-12587626	MS.gene35808:intron	35.0%
	ATTGGAAGATCAGCTCAACA+AGG	-	chr2.4:12587726-12587745	MS.gene35808:CDS	40.0%
	GTGGATTCCAAATGAAGTTG+TGG	-	chr2.4:12587626-12587645	MS.gene35808:intron	40.0%
!	GCTCCAATTTTCACAACAGT+TGG	+	chr2.4:12587684-12587703	None:intergenic	40.0%
!!	ATGGAGGTGTTAGGAGTAAA+GGG	-	chr2.4:12587579-12587598	MS.gene35808:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	12587579	12587859	12587579	ID=MS.gene35808
chr2.4	mRNA	12587579	12587859	12587579	ID=MS.gene35808.t1;Parent=MS.gene35808
chr2.4	exon	12587668	12587859	12587668	ID=MS.gene35808.t1.exon1;Parent=MS.gene35808.t1
chr2.4	CDS	12587668	12587859	12587668	ID=cds.MS.gene35808.t1;Parent=MS.gene35808.t1
chr2.4	exon	12587579	12587599	12587579	ID=MS.gene35808.t1.exon2;Parent=MS.gene35808.t1
chr2.4	CDS	12587579	12587599	12587579	ID=cds.MS.gene35808.t1;Parent=MS.gene35808.t1

Gene Sequence	>MS.gene35808 ATGGAGGTGTTAGGAGTAAAGGGTTTTGTCCTCTTGTTTTTCTGTTTGTGGATTCCAAATGAAGTTGTGGCTATAATTGGAAACTCTACTGTTTCTTCTAGACCAACTGTTGTGAAAATTGGAGCACTGTTTACTGTTGATTCTGTGATTGGAAGATCAGCTCAACAAGGAATCAAAACTGCAATTGATGATGTTAATGCTAATAAGACTATTCTTCCTGGTATTAAAATGGATGTTATTTTTCATGATACAAATTGCAGTGGATTTATTGGAACAGTTGA
Protein sequence	>MS.gene35808.t1 MEVLGVKGFVLLFFCLWIPNEVVAIIGNSTVSSRPTVVKIGALFTVDSVIGRSAQQGIKTAIDDWIYWNS