AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45194


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45194.t1 MTR_5g037140 98.750 80 0 1 1 79 45 124 9.76e-54 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45194.t1 AT1G69970 72.727 33 8 1 48 79 70 102 9.88e-11 53.5

Find 20 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGGAGAGCTGGAAATTC+TGG 0.279686 3.1:+86432377 MS.gene45194:intron
TAAACAAACCTGTTGTGAAT+AGG 0.400300 3.1:-86430721 None:intergenic
TTAGTTAGTGGTGGAGGAAC+AGG 0.433994 3.1:+86430550 MS.gene45194:CDS
TTTAGTAGCTTAGTTAGTGG+TGG 0.443725 3.1:+86430541 MS.gene45194:CDS
TGTCAACCATGATGCACAAT+TGG 0.454537 3.1:+86430654 MS.gene45194:CDS
GACCAGATCCTATTCACAAC+AGG 0.455835 3.1:+86430713 MS.gene45194:CDS
CATGATGAACAAGTTGTTGT+TGG 0.456308 3.1:+86430613 MS.gene45194:CDS
TGAATAGGATCTGGTCCATT+GGG 0.478116 3.1:-86430706 None:intergenic
GGAAATTCTGGTAGACCTCC+TGG 0.500969 3.1:+86432389 MS.gene45194:CDS
AGTAGCTTAGTTAGTGGTGG+AGG 0.503163 3.1:+86430544 MS.gene45194:CDS
AACCTGTTGTGAATAGGATC+TGG 0.513984 3.1:-86430715 None:intergenic
GTGAATAGGATCTGGTCCAT+TGG 0.561168 3.1:-86430707 None:intergenic
TGAAGTCCAATTGTGCATCA+TGG 0.582511 3.1:-86430660 None:intergenic
TCAGTTGACTAAGTTTGACC+AGG 0.583639 3.1:-86432407 None:intergenic
AGTTGTTGTTGGTAAAGACA+AGG 0.593670 3.1:+86430624 MS.gene45194:CDS
GACAAACAACTACAAGACAT+TGG 0.623172 3.1:+86430572 MS.gene45194:CDS
AACTACAAGACATTGGTCAG+AGG 0.649811 3.1:+86430579 MS.gene45194:CDS
GTTGTTGTTGGTAAAGACAA+GGG 0.652393 3.1:+86430625 MS.gene45194:CDS
AGTAAAAGAAGAGTTCCCAA+TGG 0.653384 3.1:+86430691 MS.gene45194:CDS
GTTGACTAAGTTTGACCAGG+AGG 0.677258 3.1:-86432404 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATACAATATTTAGAAAAT+AGG + chr3.1:86430959-86430978 MS.gene45194:intron 10.0%
!! TTTGAAGATATTTAATATAT+AGG - chr3.1:86432088-86432107 None:intergenic 10.0%
!!! TTTTGGATTTTTTTTTTTTT+TGG + chr3.1:86431465-86431484 MS.gene45194:intron 10.0%
!! AAAAAAAGAGATAAAAACTA+AGG + chr3.1:86431337-86431356 MS.gene45194:intron 15.0%
!! AAAAAAGAGATAAAAACTAA+GGG + chr3.1:86431338-86431357 MS.gene45194:intron 15.0%
!! ATTATAGATGAATAATATGT+TGG - chr3.1:86432297-86432316 None:intergenic 15.0%
!! TATCTGTTAATCTATTATAA+AGG - chr3.1:86431722-86431741 None:intergenic 15.0%
!! TGACAAATTTATTTAATGTA+AGG - chr3.1:86431994-86432013 None:intergenic 15.0%
!!! GAATTTTGAAATAGATATTT+AGG - chr3.1:86431962-86431981 None:intergenic 15.0%
!!! TCTTTTTTTTTTTTTTTGAG+TGG - chr3.1:86431326-86431345 None:intergenic 15.0%
!! AAGAAAGACAAAAAAAAAGA+AGG - chr3.1:86431863-86431882 None:intergenic 20.0%
!! AATCTAATTCGATTAGATTA+GGG + chr3.1:86431185-86431204 MS.gene45194:intron 20.0%
!! ATTAAATATCTTCAAATGCT+TGG + chr3.1:86432092-86432111 MS.gene45194:intron 20.0%
!! CACAAATTATTAACAAATTC+AGG + chr3.1:86432032-86432051 MS.gene45194:intron 20.0%
!! GATATATAAGTGAAAAGAAA+AGG + chr3.1:86431360-86431379 MS.gene45194:intron 20.0%
!! TATTTAGAAAATAGGATATG+AGG + chr3.1:86430967-86430986 MS.gene45194:intron 20.0%
!! TGAAATAGATATTTAGGAAA+TGG - chr3.1:86431956-86431975 None:intergenic 20.0%
!! TTAAATATCTTCAAATGCTT+GGG + chr3.1:86432093-86432112 MS.gene45194:intron 20.0%
!!! AACTTTTGATATTATGCTAA+GGG + chr3.1:86431289-86431308 MS.gene45194:intron 20.0%
!!! ACGTATTAAACATTAGTTTT+TGG + chr3.1:86431448-86431467 MS.gene45194:intron 20.0%
!!! TAACTTTTGATATTATGCTA+AGG + chr3.1:86431288-86431307 MS.gene45194:intron 20.0%
!!! TCATTTGGTAACATTTTTTT+TGG + chr3.1:86432345-86432364 MS.gene45194:intron 20.0%
!!! TTTTTTGGTAACATTTTTTC+AGG + chr3.1:86432360-86432379 MS.gene45194:intron 20.0%
! AAATCTTTATCCTGCTATAT+TGG + chr3.1:86431595-86431614 MS.gene45194:intron 25.0%
! AAGAAGATCGAATCTAATTA+AGG - chr3.1:86431513-86431532 None:intergenic 25.0%
! ACTAATGTTTAATACGTGTT+AGG - chr3.1:86431445-86431464 None:intergenic 25.0%
! CAATCTAATTCGATTAGATT+AGG + chr3.1:86431184-86431203 MS.gene45194:intron 25.0%
! GCTTATGAGTAATTAAGAAA+TGG - chr3.1:86431791-86431810 None:intergenic 25.0%
! TAAATATCTTCAAATGCTTG+GGG + chr3.1:86432094-86432113 MS.gene45194:intron 25.0%
! TACCAAATGATCTCTTAAAA+GGG - chr3.1:86432335-86432354 None:intergenic 25.0%
! TTACCAAATGATCTCTTAAA+AGG - chr3.1:86432336-86432355 None:intergenic 25.0%
! TTGGTTCAATCAGTTTAAAT+CGG + chr3.1:86431147-86431166 MS.gene45194:intron 25.0%
!! AGCTCACAAAATTTTAAATG+CGG + chr3.1:86432180-86432199 MS.gene45194:intron 25.0%
!! GTTTAAATCGGTTCAATTAA+AGG + chr3.1:86431159-86431178 MS.gene45194:intron 25.0%
!! TTAACCTCTTTTAACATTGA+TGG + chr3.1:86431695-86431714 MS.gene45194:intron 25.0%
!!! AGATTTTAAAGTCAGAGTTA+AGG - chr3.1:86431581-86431600 None:intergenic 25.0%
!!! CTTCAATTGTTTTTAGTTCA+TGG + chr3.1:86432061-86432080 MS.gene45194:intron 25.0%
!!! TAGTTAGCTTTCTTTCTTTT+TGG - chr3.1:86431629-86431648 None:intergenic 25.0%
!!! TTCAATTTTTGCCACTTATT+AGG + chr3.1:86430796-86430815 MS.gene45194:intron 25.0%
ACTGATTGAACCAATTCAAT+CGG - chr3.1:86431141-86431160 None:intergenic 30.0%
AGCAATGTATACCTAATAAG+TGG - chr3.1:86430810-86430829 None:intergenic 30.0%
CTATATTCTAGGTGCAATAT+AGG + chr3.1:86430917-86430936 MS.gene45194:intron 30.0%
GGTATGTGATAAGAATAGAA+AGG - chr3.1:86431492-86431511 None:intergenic 30.0%
GTTAAAAAATCAAACCGAAG+AGG - chr3.1:86431272-86431291 None:intergenic 30.0%
TAAACAAACCTGTTGTGAAT+AGG - chr3.1:86430724-86430743 None:intergenic 30.0%
TATGTGACACTCTATATTCT+AGG + chr3.1:86430906-86430925 MS.gene45194:intron 30.0%
TGTTAGGAAAGGAACATAAT+TGG - chr3.1:86431429-86431448 None:intergenic 30.0%
TGTTTAATACGTGTTAGGAA+AGG - chr3.1:86431440-86431459 None:intergenic 30.0%
TTCAGAGAGAAAAAATGACA+TGG - chr3.1:86431826-86431845 None:intergenic 30.0%
! AAATTTTAAATGCGGAGAAG+TGG + chr3.1:86432188-86432207 MS.gene45194:intron 30.0%
! AATTTTAAATGCGGAGAAGT+GGG + chr3.1:86432189-86432208 MS.gene45194:intron 30.0%
!! TTCCCTTTTAAGAGATCATT+TGG + chr3.1:86432330-86432349 MS.gene45194:intron 30.0%
!!! GTTTTTAGTAGCTTAGTTAG+TGG + chr3.1:86430538-86430557 MS.gene45194:CDS 30.0%
ACATTGCATAATATATGCGG+GGG - chr3.1:86432237-86432256 None:intergenic 35.0%
AGGACCATCAATGTTAAAAG+AGG - chr3.1:86431702-86431721 None:intergenic 35.0%
AGTAAAAGAAGAGTTCCCAA+TGG + chr3.1:86430691-86430710 MS.gene45194:CDS 35.0%
ATGAATAATATGTTGGTCCC+TGG - chr3.1:86432290-86432309 None:intergenic 35.0%
CATGATGAACAAGTTGTTGT+TGG + chr3.1:86430613-86430632 MS.gene45194:CDS 35.0%
CCAAACAACTGGTCAATTAT+TGG + chr3.1:86431228-86431247 MS.gene45194:intron 35.0%
CCAATAATTGACCAGTTGTT+TGG - chr3.1:86431231-86431250 None:intergenic 35.0%
CCAATTGAACCATAACTCAA+AGG - chr3.1:86431007-86431026 None:intergenic 35.0%
GACAAACAACTACAAGACAT+TGG + chr3.1:86430572-86430591 MS.gene45194:CDS 35.0%
GACATTGCATAATATATGCG+GGG - chr3.1:86432238-86432257 None:intergenic 35.0%
GATTAGATTAGGGTTCAAAC+AGG + chr3.1:86431195-86431214 MS.gene45194:intron 35.0%
GGACATTGCATAATATATGC+GGG - chr3.1:86432239-86432258 None:intergenic 35.0%
GGGACATTGCATAATATATG+CGG - chr3.1:86432240-86432259 None:intergenic 35.0%
TAATTGACCAGTTGTTTGGT+TGG - chr3.1:86431227-86431246 None:intergenic 35.0%
TTTAGTAGCTTAGTTAGTGG+TGG + chr3.1:86430541-86430560 MS.gene45194:CDS 35.0%
! ACCAATTCAATCGGTTCAAT+CGG - chr3.1:86431132-86431151 None:intergenic 35.0%
! AGTTGTTGTTGGTAAAGACA+AGG + chr3.1:86430624-86430643 MS.gene45194:CDS 35.0%
! ATGGTTCAATTGGTTTATCG+TGG + chr3.1:86431014-86431033 MS.gene45194:intron 35.0%
! ATTTTAAATGCGGAGAAGTG+GGG + chr3.1:86432190-86432209 MS.gene45194:intron 35.0%
! CCTTTGAGTTATGGTTCAAT+TGG + chr3.1:86431004-86431023 MS.gene45194:intron 35.0%
! GTTGTTGTTGGTAAAGACAA+GGG + chr3.1:86430625-86430644 MS.gene45194:CDS 35.0%
!! ACTAGTTGATTTGACCTCTT+CGG + chr3.1:86431255-86431274 MS.gene45194:intron 35.0%
!!! TAACATTTTTTCAGGAGAGC+TGG + chr3.1:86432368-86432387 MS.gene45194:intron 35.0%
AACCTGTTGTGAATAGGATC+TGG - chr3.1:86430718-86430737 None:intergenic 40.0%
AACTACAAGACATTGGTCAG+AGG + chr3.1:86430579-86430598 MS.gene45194:CDS 40.0%
ACCGATTGAACCGATTGAAT+TGG + chr3.1:86431128-86431147 MS.gene45194:intron 40.0%
GATCAAACCAACCAAACAAC+TGG + chr3.1:86431217-86431236 MS.gene45194:intron 40.0%
GGTCAAGTTTCCAATATAGC+AGG - chr3.1:86431608-86431627 None:intergenic 40.0%
TGAAGTCCAATTGTGCATCA+TGG - chr3.1:86430663-86430682 None:intergenic 40.0%
TGAATAGGATCTGGTCCATT+GGG - chr3.1:86430709-86430728 None:intergenic 40.0%
TGTCAACCATGATGCACAAT+TGG + chr3.1:86430654-86430673 MS.gene45194:CDS 40.0%
TTGTCAAGTACCCTAGTAGT+TGG + chr3.1:86432157-86432176 MS.gene45194:intron 40.0%
TTTGTGAGCTCCAACTACTA+GGG - chr3.1:86432170-86432189 None:intergenic 40.0%
! CTCATCTGACCTTTGAGTTA+TGG + chr3.1:86430995-86431014 MS.gene45194:intron 40.0%
! TTTTGTGAGCTCCAACTACT+AGG - chr3.1:86432171-86432190 None:intergenic 40.0%
AGTAGCTTAGTTAGTGGTGG+AGG + chr3.1:86430544-86430563 MS.gene45194:CDS 45.0%
GACCAGATCCTATTCACAAC+AGG + chr3.1:86430713-86430732 MS.gene45194:CDS 45.0%
GTGAATAGGATCTGGTCCAT+TGG - chr3.1:86430710-86430729 None:intergenic 45.0%
TACCAATTGAGCCAGCTTCA+CGG + chr3.1:86432260-86432279 MS.gene45194:intron 45.0%
TTCAGGAGAGCTGGAAATTC+TGG + chr3.1:86432377-86432396 MS.gene45194:intron 45.0%
! TGAAGCTGGCTCAATTGGTA+GGG - chr3.1:86432260-86432279 None:intergenic 45.0%
! TTAGTTAGTGGTGGAGGAAC+AGG + chr3.1:86430550-86430569 MS.gene45194:CDS 45.0%
ACCAATTGAGCCAGCTTCAC+GGG + chr3.1:86432261-86432280 MS.gene45194:intron 50.0%
GGAAATTCTGGTAGACCTCC+TGG + chr3.1:86432389-86432408 MS.gene45194:CDS 50.0%
TGCATAATATATGCGGGGGC+CGG - chr3.1:86432233-86432252 None:intergenic 50.0%
! GTGAAGCTGGCTCAATTGGT+AGG - chr3.1:86432261-86432280 None:intergenic 50.0%
CAATTGAGCCAGCTTCACGG+GGG + chr3.1:86432263-86432282 MS.gene45194:intron 55.0%
CATAATATATGCGGGGGCCG+GGG - chr3.1:86432231-86432250 None:intergenic 55.0%
CCAATTGAGCCAGCTTCACG+GGG + chr3.1:86432262-86432281 MS.gene45194:intron 55.0%
GCATAATATATGCGGGGGCC+GGG - chr3.1:86432232-86432251 None:intergenic 55.0%
! ATGCGGAGAAGTGGGGTGTT+CGG + chr3.1:86432197-86432216 MS.gene45194:intron 55.0%
! CCCCGTGAAGCTGGCTCAAT+TGG - chr3.1:86432265-86432284 None:intergenic 60.0%
! TGCGGAGAAGTGGGGTGTTC+GGG + chr3.1:86432198-86432217 MS.gene45194:intron 60.0%
GGTGTTCGGGGTTCGAACCC+CGG + chr3.1:86432211-86432230 MS.gene45194:intron 65.0%
! GCGGAGAAGTGGGGTGTTCG+GGG + chr3.1:86432199-86432218 MS.gene45194:intron 65.0%
AGCCAGCTTCACGGGGGACC+AGG + chr3.1:86432269-86432288 MS.gene45194:intron 70.0%
GCCAGCTTCACGGGGGACCA+GGG + chr3.1:86432270-86432289 MS.gene45194:intron 70.0%
TCCCTGGTCCCCCGTGAAGC+TGG - chr3.1:86432274-86432293 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 86430535 86432421 86430535 ID=MS.gene45194
chr3.1 mRNA 86430535 86432421 86430535 ID=MS.gene45194.t1;Parent=MS.gene45194
chr3.1 exon 86430535 86430734 86430535 ID=MS.gene45194.t1.exon1;Parent=MS.gene45194.t1
chr3.1 CDS 86430535 86430734 86430535 ID=cds.MS.gene45194.t1;Parent=MS.gene45194.t1
chr3.1 exon 86432382 86432421 86432382 ID=MS.gene45194.t1.exon2;Parent=MS.gene45194.t1
chr3.1 CDS 86432382 86432421 86432382 ID=cds.MS.gene45194.t1;Parent=MS.gene45194.t1
Gene Sequence

>MS.gene45194

ATGGTTTTTAGTAGCTTAGTTAGTGGTGGAGGAACAGGACAAACAACTACAAGACATTGGTCAGAGGAAACACTCAAACATGATGAACAAGTTGTTGTTGGTAAAGACAAGGGTGTTGTTGTCAACCATGATGCACAATTGGACTTCAACTATATGAGTAAAAGAAGAGTTCCCAATGGACCAGATCCTATTCACAACAGGTTTGTTTAGTTTATGTCTATAAACAATTTATATTATAATGTTCATATCTGTCTTTTTAATTTCAATTTTTGCCACTTATTAGGTATACATTGCTTTTAATAAAAAAATAAGAACATATATTTATCTTCTGTTTAGTAATAAAAAAACTTGTGTTTTACTTTGCTAATTAATATGTGACACTCTATATTCTAGGTGCAATATAGGTTATAGTAAGTATAGTATATTATACAATATTTAGAAAATAGGATATGAGGTTTAGCTCATCTGACCTTTGAGTTATGGTTCAATTGGTTTATCGTGGTTGAACTGAATAATGACTAACAAATAAATAGAAAGTAAATAAATATTAAAAGAGTATATAAAAGTAAACATCAAATGAAAAAAATATAAAAACCGATTGAACCGATTGAATTGGTTCAATCAGTTTAAATCGGTTCAATTAAAGGTTCAATCTAATTCGATTAGATTAGGGTTCAAACAGGATCAAACCAACCAAACAACTGGTCAATTATTGGTTCAACTAGTTGATTTGACCTCTTCGGTTTGATTTTTTAACTTTTGATATTATGCTAAGGGCTATATAAGTGCCACTCAAAAAAAAAAAAAAAGAGATAAAAACTAAGGGATATATAAGTGAAAAGAAAAGGAAAACTAGTGCTTTTTTTGTTTGAGTTTAAATTTGATGTGAGACCAATTATGTTCCTTTCCTAACACGTATTAAACATTAGTTTTTGGATTTTTTTTTTTTTTGGTCCTTTCTATTCTTATCACATACCTTAATTAGATTCGATCTTCTTTCTCAAAGTACAATACACATATTATATGTCTAATCTTTATCTTTTCCTTAACTCTGACTTTAAAATCTTTATCCTGCTATATTGGAAACTTGACCAAAAAGAAAGAAAGCTAACTATGAAAACTGATTATCATGCTTTTAACTGTTACATTGAATTGTTGATTTAACCTCTTTTAACATTGATGGTCCTTTATAATAGATTAACAGATAAAGATATTAAAAAAAGAAGATAAAGTGTATTGTTAAGAACTTTATTCCATTTCTTAATTACTCATAAGCAATTAAAGTTATCCATGTCATTTTTTCTCTCTGAATTAACAGCTTTTCACCTTCTTTTTTTTTGTCTTTCTTTTGATATTGCTTTATTTCTTTTAGCACAAGACACAAGCATGCATGAAGTTACACTTACTATGAACTAAAACCATTTCCTAAATATCTATTTCAAAATTCTAAATAGATCCTTACATTAAATAAATTTGTCACTGAGTCTCATATCTAATCACAAATTATTAACAAATTCAGGATCTAACTTCAATTGTTTTTAGTTCATGGACCTATATATTAAATATCTTCAAATGCTTGGGGATCAATATATATATATATAATATATATATATATATATATTTTGTCAAGTACCCTAGTAGTTGGAGCTCACAAAATTTTAAATGCGGAGAAGTGGGGTGTTCGGGGTTCGAACCCCGGCCCCCGCATATATTATGCAATGTCCCTACCAATTGAGCCAGCTTCACGGGGGACCAGGGACCAACATATTATTCATCTATAATAATAACTTTGAATTTCCCTTTTAAGAGATCATTTGGTAACATTTTTTTTGGTAACATTTTTTCAGGAGAGCTGGAAATTCTGGTAGACCTCCTGGTCAAACTTAG

Protein sequence

>MS.gene45194.t1

MVFSSLVSGGGTGQTTTRHWSEETLKHDEQVVVGKDKGVVVNHDAQLDFNYMSKRRVPNGPDPIHNRRAGNSGRPPGQT