AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45550.t1 MTR_2g098010 100.000 223 0 0 1 223 1 223 1.19e-170 468
MS.gene45550.t1 MTR_5g079740 98.655 223 3 0 1 223 1 223 1.96e-168 462
MS.gene45550.t1 MTR_5g079740 98.193 166 3 0 58 223 1 166 5.57e-121 340
MS.gene45550.t1 MTR_7g088490 32.558 129 87 0 9 137 2 130 5.54e-18 79.3
MS.gene45550.t1 MTR_4g087680 32.283 127 86 0 11 137 4 130 9.44e-17 75.9
MS.gene45550.t1 MTR_3g109210 27.273 209 126 4 15 217 13 201 1.43e-15 73.2
MS.gene45550.t1 MTR_4g023520 94.118 34 2 0 58 91 1 34 5.91e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45550.t1 AT3G60820 87.444 223 28 0 1 223 1 223 2.77e-150 417
MS.gene45550.t1 AT3G60820 87.444 223 28 0 1 223 1 223 2.77e-150 417
MS.gene45550.t1 AT3G60820 86.547 223 30 0 1 223 1 223 2.62e-148 412
MS.gene45550.t1 AT1G21720 32.558 129 87 0 9 137 2 130 2.33e-18 80.5
MS.gene45550.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.70e-17 78.2
MS.gene45550.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.70e-17 78.2
MS.gene45550.t1 AT4G31300 26.794 209 127 4 15 217 12 200 6.04e-13 66.2
MS.gene45550.t1 AT4G31300 26.794 209 127 4 15 217 12 200 6.04e-13 66.2
MS.gene45550.t1 AT4G31300 24.878 205 137 3 15 217 12 201 5.88e-12 63.5

Find 54 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTACAAGTGAGAGATTT+TGG 0.149319 2.1:-6221791 None:intergenic
GGATATAGCTCTCAGGGTTC+TGG 0.295385 2.1:+6224428 MS.gene45550:CDS
TGACTAAACAACACGCTAAT+TGG 0.296923 2.1:+6221549 MS.gene45550:CDS
AAATGTGTGATGGCTTCTTC+TGG 0.302083 2.1:+6222728 MS.gene45550:CDS
TACTATTCTTTCAATGTGTT+GGG 0.329503 2.1:+6224255 MS.gene45550:CDS
GATGAATACCACCAGCATTT+AGG 0.333947 2.1:-6225004 None:intergenic
TTACTATTCTTTCAATGTGT+TGG 0.338506 2.1:+6224254 MS.gene45550:CDS
TACCCTCCATTGTTGTCATA+TGG 0.354246 2.1:-6221575 None:intergenic
AGATCTTGTGTCGCAATTGC+TGG 0.365009 2.1:+6221697 MS.gene45550:intron
GGCAGGTAAGAGGAGTGGGC+TGG 0.368090 2.1:-6224495 None:intergenic
CAGGTAAGAGGAGTGGGCTG+GGG 0.380698 2.1:-6224493 None:intergenic
GCAGGTAAGAGGAGTGGGCT+GGG 0.386622 2.1:-6224494 None:intergenic
AGGTAAGAGGAGTGGGCTGG+GGG 0.399364 2.1:-6224492 None:intergenic
GAAAGCAATTGAGCCATTGC+AGG 0.407579 2.1:-6224201 None:intergenic
ACTTCACCTGGGCAGGTAAG+AGG 0.436789 2.1:-6224505 None:intergenic
GCTATATCCCACCCTCTCAT+AGG 0.437293 2.1:-6224414 None:intergenic
CCCACTCCTCTTACCTGCCC+AGG 0.466732 2.1:+6224499 MS.gene45550:CDS
TTGTGTCGCAATTGCTGGAT+CGG 0.468844 2.1:+6221702 MS.gene45550:CDS
TTCAAGAACTTCACCTGGGC+AGG 0.478299 2.1:-6224512 None:intergenic
AAAAGCATCAGTCTTTCCTT+AGG 0.478944 2.1:-6225040 None:intergenic
TTCAGTTGGTTATCAAGAAA+CGG 0.480345 2.1:-6224467 None:intergenic
TGAAGCAGAAGCAGTTGATC+TGG 0.483304 2.1:+6224635 MS.gene45550:CDS
TGGTATTCATCGTGAGTTCA+TGG 0.483816 2.1:+6225016 MS.gene45550:CDS
GTCTTCACTTATGATGCTGT+TGG 0.484095 2.1:+6224389 MS.gene45550:CDS
GAGGGTGGGATATAGCTCTC+AGG 0.498734 2.1:+6224421 MS.gene45550:CDS
GCGAGTGAGGATGTTGTAAC+CGG 0.513102 2.1:-6221761 None:intergenic
GCAGATGAGCTGCCCTGCAA+TGG 0.526195 2.1:+6224188 MS.gene45550:CDS
AACACATTGAAAGAATAGTA+AGG 0.535290 2.1:-6224252 None:intergenic
CCTGGGCAGGTAAGAGGAGT+GGG 0.542041 2.1:-6224499 None:intergenic
TGCTTCAGACAGTGGAGTAA+CGG 0.553618 2.1:-6224619 None:intergenic
ATCGTGAGTTCATGGACCTA+AGG 0.553653 2.1:+6225024 MS.gene45550:CDS
ATGCTGTTGGTTCCTATGAG+AGG 0.566800 2.1:+6224402 MS.gene45550:CDS
TGATGTGAAGGCTTTGCAGA+AGG 0.566876 2.1:+6222760 MS.gene45550:CDS
ACCTGGGCAGGTAAGAGGAG+TGG 0.577674 2.1:-6224500 None:intergenic
ATTAGCGTGTTGTTTAGTCA+TGG 0.578211 2.1:-6221546 None:intergenic
CTCCATATGACAACAATGGA+GGG 0.584655 2.1:+6221573 MS.gene45550:CDS
GTGTGATCGCAGCTGATACA+AGG 0.586277 2.1:+6221731 MS.gene45550:CDS
TATTCTTTCAATGTGTTGGG+CGG 0.587319 2.1:+6224258 MS.gene45550:CDS
GAAATTGTCATCCTAAATGC+TGG 0.591622 2.1:+6224993 MS.gene45550:CDS
ACTGCTTCTGCTTCAGACAG+TGG 0.591728 2.1:-6224627 None:intergenic
AGGGTGGGATATAGCTCTCA+GGG 0.602013 2.1:+6224422 MS.gene45550:CDS
AAAGCAATTGAGCCATTGCA+GGG 0.602125 2.1:-6224200 None:intergenic
TGTTGGTTCCTATGAGAGGG+TGG 0.605372 2.1:+6224406 MS.gene45550:CDS
AAGAAACGGCATAATGAGAG+TGG 0.608965 2.1:-6224453 None:intergenic
ATAGCTCACCTTCACTGTCA+AGG 0.612785 2.1:-6224281 None:intergenic
TGCTGTTGGTTCCTATGAGA+GGG 0.619026 2.1:+6224403 MS.gene45550:CDS
AGTGAGGATGTTGTAACCGG+TGG 0.625722 2.1:-6221758 None:intergenic
TCTCCATATGACAACAATGG+AGG 0.635572 2.1:+6221572 MS.gene45550:CDS
GTTGGTTCCTATGAGAGGGT+GGG 0.639673 2.1:+6224407 MS.gene45550:CDS
TGGTCTCCATATGACAACAA+TGG 0.646174 2.1:+6221569 MS.gene45550:CDS
GCTGATACAAGGATGTCCAC+CGG 0.646826 2.1:+6221742 MS.gene45550:CDS
TTGGGCGGCCTTGACAGTGA+AGG 0.657648 2.1:+6224273 MS.gene45550:CDS
CAGAGCTGAGAAATGTGTGA+TGG 0.663843 2.1:+6222718 MS.gene45550:intron
ATTGTCATCCTAAATGCTGG+TGG 0.706189 2.1:+6224996 MS.gene45550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr2.1:6221836-6221855 MS.gene45550:intron 0.0%
!! TATATATATATATATATATA+GGG + chr2.1:6221837-6221856 MS.gene45550:intron 0.0%
!! AATTGAAAAATATTAAAACA+AGG - chr2.1:6221648-6221667 None:intergenic 10.0%
!! AGAAGAAAAAAAAAAAAAAA+CGG - chr2.1:6222104-6222123 None:intergenic 10.0%
!! ATTGAAAAATATTAAAACAA+GGG - chr2.1:6221647-6221666 None:intergenic 10.0%
!! AAGAAAAAAAAAAAAAAACG+GGG - chr2.1:6222102-6222121 None:intergenic 15.0%
!! AATATAATCTGGTAATTAAT+TGG + chr2.1:6222355-6222374 MS.gene45550:intron 15.0%
!! AGAATTAATTAATCGATAAA+AGG - chr2.1:6222257-6222276 None:intergenic 15.0%
!! ATTAAAATATGATTTATTGC+AGG + chr2.1:6224593-6224612 MS.gene45550:intron 15.0%
!! GAAGAAAAAAAAAAAAAAAC+GGG - chr2.1:6222103-6222122 None:intergenic 15.0%
!! TATGCAAAACAAATAAAATT+AGG - chr2.1:6223803-6223822 None:intergenic 15.0%
!! TTATTTATTTATCTGTTACA+AGG - chr2.1:6222826-6222845 None:intergenic 15.0%
!! TTGAAAAATATTAAAACAAG+GGG - chr2.1:6221646-6221665 None:intergenic 15.0%
!!! ATATATATATTAAGTTGTGT+AGG - chr2.1:6221826-6221845 None:intergenic 15.0%
!!! TAAATCATATTTTAATGTAG+AGG - chr2.1:6224589-6224608 None:intergenic 15.0%
!!! TAACTTTTATATAGAAAAGA+TGG - chr2.1:6223746-6223765 None:intergenic 15.0%
!!! TTATCATTTCTTTACAATTT+TGG - chr2.1:6223889-6223908 None:intergenic 15.0%
!! AAAAATAGTCCAATAAGTAT+AGG - chr2.1:6223666-6223685 None:intergenic 20.0%
!! AAAATAAATATAGAGGTGAA+TGG - chr2.1:6223606-6223625 None:intergenic 20.0%
!! AAACGTAAAACCTAATTATT+TGG - chr2.1:6222047-6222066 None:intergenic 20.0%
!! AAGGAAATAATTAAATCTGA+TGG + chr2.1:6222465-6222484 MS.gene45550:intron 20.0%
!! ACAATAATAGAAAACCAATT+GGG - chr2.1:6222569-6222588 None:intergenic 20.0%
!! AGAAAAAAAAAAAAAAACGG+GGG - chr2.1:6222101-6222120 None:intergenic 20.0%
!! ATAAACACAACTAAAAAAAG+AGG - chr2.1:6222640-6222659 None:intergenic 20.0%
!! ATTATTGTAAAACTTCTAGT+GGG + chr2.1:6222581-6222600 MS.gene45550:intron 20.0%
!! CAAAATGACAATTATGTAAA+AGG - chr2.1:6224916-6224935 None:intergenic 20.0%
!! GAACATCAAAATAAATATAG+AGG - chr2.1:6223613-6223632 None:intergenic 20.0%
!! GTTTCAGCAATATATATATA+GGG - chr2.1:6222547-6222566 None:intergenic 20.0%
!! TACAATAATAGAAAACCAAT+TGG - chr2.1:6222570-6222589 None:intergenic 20.0%
!! TAGTTAGTTACATAGTTATT+TGG + chr2.1:6223345-6223364 MS.gene45550:intron 20.0%
!! TATAGAAACATTAGAAAAGA+GGG - chr2.1:6223694-6223713 None:intergenic 20.0%
!! TATTATTGTAAAACTTCTAG+TGG + chr2.1:6222580-6222599 MS.gene45550:intron 20.0%
!! TGAAAAATATTAAAACAAGG+GGG - chr2.1:6221645-6221664 None:intergenic 20.0%
!! TTATAGAAACATTAGAAAAG+AGG - chr2.1:6223695-6223714 None:intergenic 20.0%
!! TTATAGAGTGTGTATAAATT+AGG + chr2.1:6223373-6223392 MS.gene45550:intron 20.0%
!! TTTAGATTGTAAACATATTC+AGG + chr2.1:6222851-6222870 MS.gene45550:intron 20.0%
!! TTTGGATATACAATTATACT+TGG - chr2.1:6223871-6223890 None:intergenic 20.0%
!!! ACAATTTTATGCATGATAAT+TGG - chr2.1:6222226-6222245 None:intergenic 20.0%
!!! AGTTTGTTTATGTATGAAAA+CGG + chr2.1:6224705-6224724 MS.gene45550:intron 20.0%
!!! GATTTTGTATATCAATTACA+CGG + chr2.1:6222434-6222453 MS.gene45550:intron 20.0%
!!! TTAGATGTTAGTTTGAAATT+TGG + chr2.1:6224020-6224039 MS.gene45550:intron 20.0%
! AAAAAATTGAGCTACAATCT+TGG - chr2.1:6222407-6222426 None:intergenic 25.0%
! AACACATTGAAAGAATAGTA+AGG - chr2.1:6224255-6224274 None:intergenic 25.0%
! AATATCGTAAAGTTTGTGAA+TGG + chr2.1:6223425-6223444 MS.gene45550:intron 25.0%
! AATTGGAACGATTATCAATT+TGG - chr2.1:6222209-6222228 None:intergenic 25.0%
! AGTATAAACTTCTATAAAGG+TGG - chr2.1:6223780-6223799 None:intergenic 25.0%
! AGTTATGTTAACAAATAGAG+AGG - chr2.1:6223047-6223066 None:intergenic 25.0%
! ATAAATTAGGAGAACACAAA+GGG + chr2.1:6223386-6223405 MS.gene45550:intron 25.0%
! ATAGAAACATTAGAAAAGAG+GGG - chr2.1:6223693-6223712 None:intergenic 25.0%
! ATCTAACAACGTAATTGATA+AGG - chr2.1:6224006-6224025 None:intergenic 25.0%
! ATTGTAAAGAAATGATAAGC+AGG + chr2.1:6223891-6223910 MS.gene45550:intron 25.0%
! CAAGGTAATATTCAAAACAA+TGG - chr2.1:6224094-6224113 None:intergenic 25.0%
! GGTTTCAGCAATATATATAT+AGG - chr2.1:6222548-6222567 None:intergenic 25.0%
! GTAACACATTATGTAATGAT+GGG - chr2.1:6223284-6223303 None:intergenic 25.0%
! GTATAAACTTCTATAAAGGT+GGG - chr2.1:6223779-6223798 None:intergenic 25.0%
! TAAACACTAAAGTCAAAATG+TGG - chr2.1:6224788-6224807 None:intergenic 25.0%
! TAACAAATAGAGAGGTTAAA+AGG - chr2.1:6223039-6223058 None:intergenic 25.0%
! TACTATTCTTTCAATGTGTT+GGG + chr2.1:6224255-6224274 MS.gene45550:CDS 25.0%
! TATAAATTAGGAGAACACAA+AGG + chr2.1:6223385-6223404 MS.gene45550:intron 25.0%
! TCGTTTCTCATATAAGATAT+TGG + chr2.1:6222504-6222523 MS.gene45550:intron 25.0%
! TCGTTTCTCATATAAGATAT+TGG - chr2.1:6222523-6222504 None:intergenic 25.0%
! TTAAGAAAATAAGAAAGACC+AGG - chr2.1:6223499-6223518 None:intergenic 25.0%
! TTACTATTCTTTCAATGTGT+TGG + chr2.1:6224254-6224273 MS.gene45550:CDS 25.0%
! TTATTGTAAAACTTCTAGTG+GGG + chr2.1:6222582-6222601 MS.gene45550:intron 25.0%
!! AGAAAGCGTAAAATATAATC+TGG + chr2.1:6222344-6222363 MS.gene45550:intron 25.0%
!! AGGAGTATAAACTTCTATAA+AGG - chr2.1:6223783-6223802 None:intergenic 25.0%
!! CAATTACACAGTATGTTTTA+AGG - chr2.1:6221956-6221975 None:intergenic 25.0%
!! CTTTAACTTCAACCTAAAAT+AGG + chr2.1:6224356-6224375 MS.gene45550:intron 25.0%
!! GAAGCTTTAATTATTTGTCA+AGG - chr2.1:6224112-6224131 None:intergenic 25.0%
!! TTTGAAATTTGGTGAAATTG+TGG + chr2.1:6224031-6224050 MS.gene45550:intron 25.0%
!!! CATAATTGTCATTTTGCATT+AGG + chr2.1:6224920-6224939 MS.gene45550:intron 25.0%
!!! GTGTTTTTCTTGAAGTATTT+TGG + chr2.1:6224538-6224557 MS.gene45550:intron 25.0%
!!! GTTAAATGTTGCTTTGATTT+GGG + chr2.1:6223110-6223129 MS.gene45550:intron 25.0%
AACAAATTGAGCTACAATCT+TGG + chr2.1:6221995-6222014 MS.gene45550:intron 30.0%
AACCTAATTATTTGGAGAAG+AGG - chr2.1:6222039-6222058 None:intergenic 30.0%
ACAAACAGAACAAAAACACA+AGG - chr2.1:6222924-6222943 None:intergenic 30.0%
ACTTCAACCTAAAATAGGAA+AGG + chr2.1:6224361-6224380 MS.gene45550:intron 30.0%
ATATATTGCTGAAACCCAAT+TGG + chr2.1:6222552-6222571 MS.gene45550:intron 30.0%
ATCAGATTTAATTAACGCGT+AGG + chr2.1:6221918-6221937 MS.gene45550:intron 30.0%
ATTCTATATACCGAGAAAAG+AGG - chr2.1:6223239-6223258 None:intergenic 30.0%
CGTAACACATTATGTAATGA+TGG - chr2.1:6223285-6223304 None:intergenic 30.0%
CTTCAACCTAAAATAGGAAA+GGG + chr2.1:6224362-6224381 MS.gene45550:intron 30.0%
GAAACGAGAACTATCTATAT+CGG + chr2.1:6221893-6221912 MS.gene45550:intron 30.0%
GTAAACATATTCAGGAAACA+GGG + chr2.1:6222859-6222878 MS.gene45550:intron 30.0%
GTCCAATAAGTATAGGTAAA+AGG - chr2.1:6223659-6223678 None:intergenic 30.0%
TACATGACATGCATTATGTT+AGG - chr2.1:6223559-6223578 None:intergenic 30.0%
TATTCAAAACAATGGAAGCA+TGG - chr2.1:6224086-6224105 None:intergenic 30.0%
TGTAAACATATTCAGGAAAC+AGG + chr2.1:6222858-6222877 MS.gene45550:intron 30.0%
TTCAGTTGGTTATCAAGAAA+CGG - chr2.1:6224470-6224489 None:intergenic 30.0%
TTCCTCTTCTCCAAATAATT+AGG + chr2.1:6222034-6222053 MS.gene45550:intron 30.0%
! AAAGGTTTGAGATTTGGAAA+TGG + chr2.1:6222300-6222319 MS.gene45550:intron 30.0%
! AAGTCTAAACTAGGTTTTTG+GGG + chr2.1:6222676-6222695 MS.gene45550:intron 30.0%
! ATATATATAGGGAGCATATC+CGG + chr2.1:6221848-6221867 MS.gene45550:intron 30.0%
! ATGTCATGTATTGAGTTGTA+AGG + chr2.1:6223569-6223588 MS.gene45550:intron 30.0%
! CAAGATTGAGAAATTTCTAG+CGG - chr2.1:6222176-6222195 None:intergenic 30.0%
! CAAGTCTAAACTAGGTTTTT+GGG + chr2.1:6222675-6222694 MS.gene45550:intron 30.0%
! CAATTACACGGTATGTTTTA+AGG + chr2.1:6222446-6222465 MS.gene45550:intron 30.0%
! CTCCTTTTACCTATACTTAT+TGG + chr2.1:6223654-6223673 MS.gene45550:intron 30.0%
! TCACTATGTTGATTACTTGT+TGG + chr2.1:6222949-6222968 MS.gene45550:intron 30.0%
! TTGATTACTTGTTGGAAAGT+TGG + chr2.1:6222957-6222976 MS.gene45550:intron 30.0%
!! ACAAGCTTTAAACTTGCTTT+GGG + chr2.1:6224943-6224962 MS.gene45550:intron 30.0%
!! GGTTAAATGTTGCTTTGATT+TGG + chr2.1:6223109-6223128 MS.gene45550:intron 30.0%
!! TATAAGATATTGGTTCTCAC+CGG - chr2.1:6221870-6221889 None:intergenic 30.0%
!! TTCTGCACTAGTATTTTTGT+TGG + chr2.1:6223630-6223649 MS.gene45550:intron 30.0%
!! TTTGGGTGTATGTTTTTACA+GGG + chr2.1:6224960-6224979 MS.gene45550:intron 30.0%
!!! GTATTTTGACTTTGCAAAAC+AGG - chr2.1:6224814-6224833 None:intergenic 30.0%
AAAGATGCCAAGTCTAAACT+AGG + chr2.1:6222667-6222686 MS.gene45550:intron 35.0%
AAAGCAACATTTAACCCTAG+AGG - chr2.1:6223105-6223124 None:intergenic 35.0%
AAATGATAAGCAGGATTGAG+GGG + chr2.1:6223900-6223919 MS.gene45550:intron 35.0%
AAGATCGTGGACATGTTTAA+AGG - chr2.1:6223721-6223740 None:intergenic 35.0%
AGAAATGATAAGCAGGATTG+AGG + chr2.1:6223898-6223917 MS.gene45550:intron 35.0%
AGAGCGAGCAAATACAAAAA+TGG - chr2.1:6224334-6224353 None:intergenic 35.0%
ATTGAGGGGAAAGTAAAAAC+TGG + chr2.1:6223914-6223933 MS.gene45550:intron 35.0%
ATTTCGACTGCAGAAAATTG+TGG + chr2.1:6224889-6224908 MS.gene45550:intron 35.0%
CACATTCATACTTGCAGAAT+TGG + chr2.1:6224742-6224761 MS.gene45550:intron 35.0%
CCAAAAACCTAGTTTAGACT+TGG - chr2.1:6222677-6222696 None:intergenic 35.0%
CTTTCTTGAAAGAGAGCAAA+AGG - chr2.1:6222143-6222162 None:intergenic 35.0%
GAAATGATAAGCAGGATTGA+GGG + chr2.1:6223899-6223918 MS.gene45550:intron 35.0%
GAAATTGTCATCCTAAATGC+TGG + chr2.1:6224993-6225012 MS.gene45550:CDS 35.0%
GAGCGAGCAAATACAAAAAT+GGG - chr2.1:6224333-6224352 None:intergenic 35.0%
GGTTTATTTATAGACTGCCA+TGG + chr2.1:6223203-6223222 MS.gene45550:intron 35.0%
TATTCTTTCAATGTGTTGGG+CGG + chr2.1:6224258-6224277 MS.gene45550:CDS 35.0%
TGACTAAACAACACGCTAAT+TGG + chr2.1:6221549-6221568 MS.gene45550:CDS 35.0%
TTATTTGGAGAAGAGGAACT+CGG - chr2.1:6222032-6222051 None:intergenic 35.0%
TTCTATTCCATTCAGCTCTT+GGG + chr2.1:6223531-6223550 MS.gene45550:intron 35.0%
TTTCTATTCCATTCAGCTCT+TGG + chr2.1:6223530-6223549 MS.gene45550:intron 35.0%
! ACACATCCCTTTCCTATTTT+AGG - chr2.1:6224371-6224390 None:intergenic 35.0%
! AGCTTACAAGTGAGAGATTT+TGG - chr2.1:6221794-6221813 None:intergenic 35.0%
! ATACCGAGAAAAGAGGTTTT+CGG - chr2.1:6223232-6223251 None:intergenic 35.0%
! CCAAGTCTAAACTAGGTTTT+TGG + chr2.1:6222674-6222693 MS.gene45550:intron 35.0%
! GAACGATTATCAATTTGGTG+AGG - chr2.1:6222204-6222223 None:intergenic 35.0%
! TAACCGAAAACCTCTTTTCT+CGG + chr2.1:6223226-6223245 MS.gene45550:intron 35.0%
! TGAGGAAAAGGTTTGAGATT+TGG + chr2.1:6222294-6222313 MS.gene45550:intron 35.0%
!! AAACTGAGAGTATTCCTCTA+GGG + chr2.1:6223088-6223107 MS.gene45550:intron 35.0%
!! AAGCAGTGTTGATCATTTGA+TGG + chr2.1:6223182-6223201 MS.gene45550:intron 35.0%
!! CTTTGGGTGTATGTTTTTAC+AGG + chr2.1:6224959-6224978 MS.gene45550:intron 35.0%
!! GACAAGCTTTAAACTTGCTT+TGG + chr2.1:6224942-6224961 MS.gene45550:intron 35.0%
!! TAAACTGAGAGTATTCCTCT+AGG + chr2.1:6223087-6223106 MS.gene45550:intron 35.0%
!! TACTTGCTGATTTGAGGAAA+AGG + chr2.1:6222282-6222301 MS.gene45550:intron 35.0%
!! TATGTTTTTACAGGGGGATA+AGG + chr2.1:6224968-6224987 MS.gene45550:intron 35.0%
!! TTGGGTGTATGTTTTTACAG+GGG + chr2.1:6224961-6224980 MS.gene45550:intron 35.0%
AAAGCAATTGAGCCATTGCA+GGG - chr2.1:6224203-6224222 None:intergenic 40.0%
AAATGTGTGATGGCTTCTTC+TGG + chr2.1:6222728-6222747 MS.gene45550:CDS 40.0%
AACATATTCAGGAAACAGGG+TGG + chr2.1:6222862-6222881 MS.gene45550:intron 40.0%
AAGAAACGGCATAATGAGAG+TGG - chr2.1:6224456-6224475 None:intergenic 40.0%
ACCAATTCAAGAACTTCACC+TGG - chr2.1:6224520-6224539 None:intergenic 40.0%
AGAAAAGATGGCAAAGATCG+TGG - chr2.1:6223734-6223753 None:intergenic 40.0%
AGTAGCAACTCTAATGCGAA+GGG + chr2.1:6223448-6223467 MS.gene45550:intron 40.0%
ATTAAAACAAGGGGGAGAGA+GGG - chr2.1:6221637-6221656 None:intergenic 40.0%
ATTGTCATCCTAAATGCTGG+TGG + chr2.1:6224996-6225015 MS.gene45550:CDS 40.0%
CCAATTCAAGAACTTCACCT+GGG - chr2.1:6224519-6224538 None:intergenic 40.0%
CTCCATATGACAACAATGGA+GGG + chr2.1:6221573-6221592 MS.gene45550:CDS 40.0%
CTGCAAGTATGAATGTGTGA+TGG - chr2.1:6224739-6224758 None:intergenic 40.0%
GATGAATACCACCAGCATTT+AGG - chr2.1:6225007-6225026 None:intergenic 40.0%
GTCTTCACTTATGATGCTGT+TGG + chr2.1:6224389-6224408 MS.gene45550:CDS 40.0%
TACCCTCCATTGTTGTCATA+TGG - chr2.1:6221578-6221597 None:intergenic 40.0%
TATTAAAACAAGGGGGAGAG+AGG - chr2.1:6221638-6221657 None:intergenic 40.0%
TCTCCATATGACAACAATGG+AGG + chr2.1:6221572-6221591 MS.gene45550:CDS 40.0%
TGGTATTCATCGTGAGTTCA+TGG + chr2.1:6225016-6225035 MS.gene45550:CDS 40.0%
TGGTCTCCATATGACAACAA+TGG + chr2.1:6221569-6221588 MS.gene45550:CDS 40.0%
TTCAACCGCCTGATCTTAAT+AGG - chr2.1:6224844-6224863 None:intergenic 40.0%
! AAAGAGGTTTTCGGTTACCA+TGG - chr2.1:6223223-6223242 None:intergenic 40.0%
! CAAAACAATGGAAGCATGGA+AGG - chr2.1:6224082-6224101 None:intergenic 40.0%
!! TAGTGCTACTTGCTGATTTG+AGG + chr2.1:6222276-6222295 MS.gene45550:intron 40.0%
!! TGGGTGTATGTTTTTACAGG+GGG + chr2.1:6224962-6224981 MS.gene45550:intron 40.0%
!! TGGTTTTCAAGCTGATGTGA+AGG + chr2.1:6222748-6222767 MS.gene45550:CDS 40.0%
AATCTCGTGACTGTGAATGC+AGG + chr2.1:6224863-6224882 MS.gene45550:intron 45.0%
ACCGCCTGATCTTAATAGGA+CGG - chr2.1:6224840-6224859 None:intergenic 45.0%
AGATCTTGTGTCGCAATTGC+TGG + chr2.1:6221697-6221716 MS.gene45550:intron 45.0%
ATAGCTCACCTTCACTGTCA+AGG - chr2.1:6224284-6224303 None:intergenic 45.0%
ATCGTGAGTTCATGGACCTA+AGG + chr2.1:6225024-6225043 MS.gene45550:CDS 45.0%
ATCTCGTGACTGTGAATGCA+GGG + chr2.1:6224864-6224883 MS.gene45550:intron 45.0%
CAAGCCGTCCTATTAAGATC+AGG + chr2.1:6224833-6224852 MS.gene45550:intron 45.0%
CAGAGCTGAGAAATGTGTGA+TGG + chr2.1:6222718-6222737 MS.gene45550:intron 45.0%
CCCAGGTGAAGTTCTTGAAT+TGG + chr2.1:6224516-6224535 MS.gene45550:intron 45.0%
GAAAGCAATTGAGCCATTGC+AGG - chr2.1:6224204-6224223 None:intergenic 45.0%
GAGTAGCAACTCTAATGCGA+AGG + chr2.1:6223447-6223466 MS.gene45550:intron 45.0%
GTAGCAACTCTAATGCGAAG+GGG + chr2.1:6223449-6223468 MS.gene45550:intron 45.0%
TAATGCGAAGGGGAAACTCT+TGG + chr2.1:6223459-6223478 MS.gene45550:intron 45.0%
TGAAGCAGAAGCAGTTGATC+TGG + chr2.1:6224635-6224654 MS.gene45550:CDS 45.0%
TGATGTGAAGGCTTTGCAGA+AGG + chr2.1:6222760-6222779 MS.gene45550:CDS 45.0%
TGCTTCAGACAGTGGAGTAA+CGG - chr2.1:6224622-6224641 None:intergenic 45.0%
TTAGGAACCCAAGAGCTGAA+TGG - chr2.1:6223541-6223560 None:intergenic 45.0%
TTGGAGGAGAGATTATCTCC+TGG + chr2.1:6223478-6223497 MS.gene45550:intron 45.0%
TTGTGTCGCAATTGCTGGAT+CGG + chr2.1:6221702-6221721 MS.gene45550:CDS 45.0%
!! ATGCTGTTGGTTCCTATGAG+AGG + chr2.1:6224402-6224421 MS.gene45550:CDS 45.0%
!! GGTTTTGTCTGCTAGGCATT+TGG + chr2.1:6222781-6222800 MS.gene45550:CDS 45.0%
!! TGCAGAAGGTTTTGTCTGCT+AGG + chr2.1:6222774-6222793 MS.gene45550:CDS 45.0%
!! TGCTGTTGGTTCCTATGAGA+GGG + chr2.1:6224403-6224422 MS.gene45550:CDS 45.0%
!!! TTTTGGAGTAATCGCGAGTG+AGG - chr2.1:6221777-6221796 None:intergenic 45.0%
ACTGCTTCTGCTTCAGACAG+TGG - chr2.1:6224630-6224649 None:intergenic 50.0%
AGGGTGGGATATAGCTCTCA+GGG + chr2.1:6224422-6224441 MS.gene45550:CDS 50.0%
AGTGAGGATGTTGTAACCGG+TGG - chr2.1:6221761-6221780 None:intergenic 50.0%
GCCGTCCTATTAAGATCAGG+CGG + chr2.1:6224836-6224855 MS.gene45550:intron 50.0%
GCGAGTGAGGATGTTGTAAC+CGG - chr2.1:6221764-6221783 None:intergenic 50.0%
GCTATATCCCACCCTCTCAT+AGG - chr2.1:6224417-6224436 None:intergenic 50.0%
GCTGATACAAGGATGTCCAC+CGG + chr2.1:6221742-6221761 MS.gene45550:CDS 50.0%
GGATATAGCTCTCAGGGTTC+TGG + chr2.1:6224428-6224447 MS.gene45550:CDS 50.0%
GTGTGATCGCAGCTGATACA+AGG + chr2.1:6221731-6221750 MS.gene45550:CDS 50.0%
TTCAAGAACTTCACCTGGGC+AGG - chr2.1:6224515-6224534 None:intergenic 50.0%
! GTTGGTTCCTATGAGAGGGT+GGG + chr2.1:6224407-6224426 MS.gene45550:CDS 50.0%
!! TGTTGGTTCCTATGAGAGGG+TGG + chr2.1:6224406-6224425 MS.gene45550:CDS 50.0%
ACTTCACCTGGGCAGGTAAG+AGG - chr2.1:6224508-6224527 None:intergenic 55.0%
GAGGGTGGGATATAGCTCTC+AGG + chr2.1:6224421-6224440 MS.gene45550:CDS 55.0%
TGCGAAGGGGAAACTCTTGG+AGG + chr2.1:6223462-6223481 MS.gene45550:intron 55.0%
AACAAGGGGGAGAGAGGGAG+AGG - chr2.1:6221632-6221651 None:intergenic 60.0%
ACAAGGGGGAGAGAGGGAGA+GGG - chr2.1:6221631-6221650 None:intergenic 60.0%
ACCTGGGCAGGTAAGAGGAG+TGG - chr2.1:6224503-6224522 None:intergenic 60.0%
CCTGGGCAGGTAAGAGGAGT+GGG - chr2.1:6224502-6224521 None:intergenic 60.0%
GCAGATGAGCTGCCCTGCAA+TGG + chr2.1:6224188-6224207 MS.gene45550:CDS 60.0%
TTGGGCGGCCTTGACAGTGA+AGG + chr2.1:6224273-6224292 MS.gene45550:CDS 60.0%
! GCAGGTAAGAGGAGTGGGCT+GGG - chr2.1:6224497-6224516 None:intergenic 60.0%
!! AGGTAAGAGGAGTGGGCTGG+GGG - chr2.1:6224495-6224514 None:intergenic 60.0%
!! CAGGTAAGAGGAGTGGGCTG+GGG - chr2.1:6224496-6224515 None:intergenic 60.0%
CCCACTCCTCTTACCTGCCC+AGG + chr2.1:6224499-6224518 MS.gene45550:CDS 65.0%
GGCAGGTAAGAGGAGTGGGC+TGG - chr2.1:6224498-6224517 None:intergenic 65.0%
!! GTGGGCTGGGGGACTTCAGT+TGG - chr2.1:6224484-6224503 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6221548 6225055 6221548 ID=MS.gene45550
chr2.1 mRNA 6221548 6225055 6221548 ID=MS.gene45550.t1;Parent=MS.gene45550
chr2.1 exon 6221548 6221594 6221548 ID=MS.gene45550.t1.exon1;Parent=MS.gene45550.t1
chr2.1 CDS 6221548 6221594 6221548 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6221699 6221806 6221699 ID=MS.gene45550.t1.exon2;Parent=MS.gene45550.t1
chr2.1 CDS 6221699 6221806 6221699 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6222721 6222802 6222721 ID=MS.gene45550.t1.exon3;Parent=MS.gene45550.t1
chr2.1 CDS 6222721 6222802 6222721 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6224165 6224294 6224165 ID=MS.gene45550.t1.exon4;Parent=MS.gene45550.t1
chr2.1 CDS 6224165 6224294 6224165 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6224378 6224520 6224378 ID=MS.gene45550.t1.exon5;Parent=MS.gene45550.t1
chr2.1 CDS 6224378 6224520 6224378 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6224615 6224701 6224615 ID=MS.gene45550.t1.exon6;Parent=MS.gene45550.t1
chr2.1 CDS 6224615 6224701 6224615 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
chr2.1 exon 6224981 6225055 6224981 ID=MS.gene45550.t1.exon7;Parent=MS.gene45550.t1
chr2.1 CDS 6224981 6225055 6224981 ID=cds.MS.gene45550.t1;Parent=MS.gene45550.t1
Gene Sequence

>MS.gene45550

ATGACTAAACAACACGCTAATTGGTCTCCATATGACAACAATGGAGGGTAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTCTCCCTCTCTCCCCCTTGTTTTAATATTTTTCAATTACTATCTTTTTAATATTATTATTATTTGTAGATCTTGTGTCGCAATTGCTGGATCGGATTACTGTGTGATCGCAGCTGATACAAGGATGTCCACCGGTTACAACATCCTCACTCGCGATTACTCCAAAATCTCTCACTTGTAAGCTATTTCTCATCCTACACAACTTAATATATATATATATATATATAGGGAGCATATCCGGTGAGAACCAATATCTTATATGAGAAACGAGAACTATCTATATCGGCTATCAGATTTAATTAACGCGTAGGATTTAATTATTTCCTTAAAACATACTGTGTAATTGATATACAAAATCACAAGTTAACAAATTGAGCTACAATCTTGGCACAATTAACGCCGAGTTCCTCTTCTCCAAATAATTAGGTTTTACGTTTCTTGTTTCTTGTTTTTCTTTATATTATTGTTCCCCCGTTTTTTTTTTTTTTTCTTCTTCTTAATATGATTTTGCCTTTTGCTCTCTTTCAAGAAAGCTAGTATATACCGCTAGAAATTTCTCAATCTTGTTTATCCTCACCAAATTGATAATCGTTCCAATTATCATGCATAAAATTGTGTCTATTACCTTTTATCGATTAATTAATTCTAGTGCTACTTGCTGATTTGAGGAAAAGGTTTGAGATTTGGAAATGGCTGCAACAAACAAGAAATCAAAGAAAGCGTAAAATATAATCTGGTAATTAATTGGAGAAGAGAAACTGAGCGCTAATTGTGCCAAGATTGTAGCTCAATTTTTTAACTTGTGATTTTGTATATCAATTACACGGTATGTTTTAAGGAAATAATTAAATCTGATGGCTGATATAGATAGTTCTCGTTTCTCATATAAGATATTGGTTCTCATCGAATATGCTCCCTATATATATATTGCTGAAACCCAATTGGTTTTCTATTATTGTAAAACTTCTAGTGGGGTTAATTTTCTTGATCATAGCATTAGACAAATGCCTCTTTTTTTAGTTGTGTTTATGCTGTTAAAAGATGCCAAGTCTAAACTAGGTTTTTGGGGTTTGTGTTTTGTTTCGCAACAGAGCTGAGAAATGTGTGATGGCTTCTTCTGGTTTTCAAGCTGATGTGAAGGCTTTGCAGAAGGTTTTGTCTGCTAGGCATTTGGTGAGTCTAATAATCTCAATCCTTGTAACAGATAAATAAATAATACTATTTAGATTGTAAACATATTCAGGAAACAGGGTGGCTTTGCATGTGAGAAACTTTCTTCTTTGTTGAATTTCCTTGTGTTTTTGTTCTGTTTGTATGCTTCACTATGTTGATTACTTGTTGGAAAGTTGGAAATATTTTCTAGATGAGTAAAGAAGTTTAGAAGAAACTTTATTCAGTTGCAAATTCCTTTTAACCTCTCTATTTGTTAACATAACTGATTTAGAACTACAGAAGAATAAACTGAGAGTATTCCTCTAGGGTTAAATGTTGCTTTGATTTGGGTTTCAGATATTCTGATAATAAACTAAACTCAATATTCTTAGTTATACAAAAGCAGTGTTGATCATTTGATGGTTTATTTATAGACTGCCATGGTAACCGAAAACCTCTTTTCTCGGTATATAGAATTAGATACATTGATTAATTGACACCCATCATTACATAATGTGTTACGATATATAATAATTGCTAATTAAATTAGTTAAGAGTTGCTATAGTTAGTTACATAGTTATTTGGATTAGTTATAGAGTGTGTATAAATTAGGAGAACACAAAGGGTAGAAAGTATCTTTTGAATATCGTAAAGTTTGTGAATGGAGTAGCAACTCTAATGCGAAGGGGAAACTCTTGGAGGAGAGATTATCTCCTGGTCTTTCTTATTTTCTTAATTCAGTTGTTCTTTCTATTCCATTCAGCTCTTGGGTTCCTAACATAATGCATGTCATGTATTGAGTTGTAAGGATTGAGATCGACCATTCACCTCTATATTTATTTTGATGTTCTGCACTAGTATTTTTGTTGGTCTCCTTTTACCTATACTTATTGGACTATTTTTGTCACCCCTCTTTTCTAATGTTTCTATAAGTTCCTTTAAACATGTCCACGATCTTTGCCATCTTTTCTATATAAAAGTTATTGAGTTAAGCCCACCTTTATAGAAGTTTATACTCCTAATTTTATTTGTTTTGCATAATCATCATCTGTTGTGTAAATTGTATTATTATCGTCTTTTTGACGCCAAGTATAATTGTATATCCAAAATTGTAAAGAAATGATAAGCAGGATTGAGGGGAAAGTAAAAACTGGCGAAGTGTTCTGTCAATTGCATTGCATCAATTATTAAAGTTTTTACTCTTTTATCAATGCATATAACCTTATCAATTACGTTGTTAGATGTTAGTTTGAAATTTGGTGAAATTGTGGTGTTTGACTCACAAACTGTTATGTACCTTCCATGCTTCCATTGTTTTGAATATTACCTTGACAAATAATTAAAGCTTCTGATTGAAATGACACTATTGATTCTTAATGCAGACTTATCAGCATCAACACAACAAGCAGATGAGCTGCCCTGCAATGGCTCAATTGCTTTCAAACACACTTTATTACAAACGCTTCTTTCCTTACTATTCTTTCAATGTGTTGGGCGGCCTTGACAGTGAAGGTGAGCTATTTCTAACTTTCTATACTTGTTCGTGTCCCATTTTTGTATTTGCTCGCTCTGACTTTAACTTCAACCTAAAATAGGAAAGGGATGTGTCTTCACTTATGATGCTGTTGGTTCCTATGAGAGGGTGGGATATAGCTCTCAGGGTTCTGGCTCCACTCTCATTATGCCGTTTCTTGATAACCAACTGAAGTCCCCCAGCCCACTCCTCTTACCTGCCCAGGTGAAGTTCTTGAATTGGTGTTTTTCTTGAAGTATTTTGGTTCTTTAAATTATTTTTGAATAACACCTCTACATTAAAATATGATTTATTGCAGGATGCCGTTACTCCACTGTCTGAAGCAGAAGCAGTTGATCTGGTCAAAACTGTTTTTGCATCTGCAACTGAGAGAGATATATACACTGTAAGTTTGTTTATGTATGAAAACGGATTGTCTGCCATCACACATTCATACTTGCAGAATTGGATCGTCTAATCTTAATCAATCCACATTTTGACTTTAGTGTTTAAGACCTGTTTTGCAAAGTCAAAATACAAGCCGTCCTATTAAGATCAGGCGGTTGAAATCTCGTGACTGTGAATGCAGGGTTATTTCGACTGCAGAAAATTGTGGTCCTTTTACATAATTGTCATTTTGCATTAGGACAAGCTTTAAACTTGCTTTGGGTGTATGTTTTTACAGGGGGATAAGGTTGAAATTGTCATCCTAAATGCTGGTGGTATTCATCGTGAGTTCATGGACCTAAGGAAAGACTGA
Protein sequence

>MS.gene45550.t1

MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD