AlfalfaGEDB | MS.gene49271

M. sativa cultivar XinJiangDaYe / MS.gene49271

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49271.t1	XP_003608847.1	97.6	41	1	0	1	41	1	41	7.50E-13	84

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49271.t1	G7JLF5	97.6	41	1	0	1	41	1	41	5.4e-13	84.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051226	MS.gene49271	0.807052	6.10E-50	-1.69E-46
MS.gene057167	MS.gene49271	0.820856	5.50E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene49271	MS.gene64311	PPI
MS.gene80011	MS.gene49271	PPI
MS.gene070168	MS.gene49271	PPI
MS.gene49271	MS.gene026940	PPI
MS.gene49271	MS.gene024717	PPI
MS.gene49271	MS.gene28338	PPI
MS.gene004899	MS.gene49271	PPI
MS.gene49271	MS.gene87673	PPI
MS.gene49271	MS.gene53659	PPI
MS.gene49271	MS.gene09960	PPI
MS.gene49271	MS.gene061768	PPI
MS.gene49271	MS.gene89216	PPI
MS.gene49271	MS.gene64308	PPI
MS.gene49271	MS.gene009021	PPI
MS.gene49271	MS.gene47195	PPI
MS.gene070167	MS.gene49271	PPI
MS.gene49271	MS.gene017446	PPI
MS.gene49271	MS.gene96914	PPI
MS.gene49271	MS.gene066862	PPI
MS.gene49271	MS.gene96913	PPI
MS.gene041795	MS.gene49271	PPI
MS.gene49271	MS.gene07422	PPI
MS.gene49271	MS.gene015658	PPI
MS.gene49271	MS.gene93763	PPI
MS.gene49271	MS.gene64313	PPI
MS.gene49271	MS.gene80012	PPI
MS.gene68345	MS.gene49271	PPI
MS.gene49271	MS.gene061261	PPI
MS.gene49271	MS.gene036930	PPI
MS.gene001246	MS.gene49271	PPI
MS.gene040127	MS.gene49271	PPI
MS.gene62258	MS.gene49271	PPI
MS.gene49271	MS.gene21932	PPI
MS.gene49271	MS.gene026618	PPI
MS.gene038456	MS.gene49271	PPI
MS.gene052672	MS.gene49271	PPI
MS.gene49271	MS.gene53648	PPI
MS.gene49271	MS.gene015470	PPI
MS.gene49271	MS.gene008831	PPI
MS.gene49271	MS.gene55545	PPI
MS.gene49271	MS.gene056461	PPI
MS.gene49271	MS.gene98972	PPI
MS.gene49271	MS.gene29459	PPI
MS.gene49271	MS.gene92775	PPI
MS.gene58860	MS.gene49271	PPI
MS.gene49271	MS.gene53662	PPI
MS.gene27516	MS.gene49271	PPI
MS.gene49271	MS.gene63281	PPI
MS.gene49271	MS.gene020515	PPI
MS.gene61009	MS.gene49271	PPI
MS.gene64327	MS.gene49271	PPI
MS.gene64307	MS.gene49271	PPI
MS.gene49271	MS.gene013748	PPI
MS.gene04917	MS.gene49271	PPI
MS.gene49271	MS.gene21696	PPI
MS.gene49271	MS.gene64316	PPI
MS.gene49271	MS.gene05284	PPI
MS.gene49271	MS.gene22553	PPI
MS.gene026530	MS.gene49271	PPI
MS.gene49271	MS.gene03228	PPI
MS.gene49271	MS.gene46237	PPI
MS.gene49271	MS.gene64317	PPI
MS.gene49271	MS.gene025777	PPI
MS.gene49271	MS.gene63000	PPI
MS.gene49271	MS.gene93087	PPI
MS.gene49271	MS.gene64310	PPI
MS.gene49271	MS.gene046988	PPI
MS.gene49271	MS.gene057293	PPI
MS.gene49271	MS.gene71207	PPI
MS.gene49271	MS.gene09934	PPI
MS.gene49271	MS.gene58906	PPI
MS.gene49271	MS.gene79028	PPI
MS.gene83068	MS.gene49271	PPI
MS.gene62689	MS.gene49271	PPI
MS.gene97948	MS.gene49271	PPI
MS.gene003880	MS.gene49271	PPI
MS.gene54637	MS.gene49271	PPI
MS.gene000738	MS.gene49271	PPI
MS.gene67281	MS.gene49271	PPI
MS.gene49271	MS.gene053319	PPI
MS.gene49271	MS.gene000724	PPI
MS.gene49271	MS.gene65954	PPI
MS.gene062239	MS.gene49271	PPI
MS.gene46886	MS.gene49271	PPI
MS.gene49271	MS.gene074045	PPI
MS.gene49271	MS.gene85596	PPI
MS.gene49271	MS.gene96922	PPI
MS.gene49271	MS.gene25186	PPI
MS.gene49271	MS.gene80013	PPI
MS.gene21155	MS.gene49271	PPI
MS.gene49271	MS.gene025797	PPI
MS.gene49271	MS.gene21931	PPI
MS.gene49271	MS.gene64314	PPI
MS.gene49271	MS.gene94551	PPI
MS.gene008225	MS.gene49271	PPI
MS.gene056383	MS.gene49271	PPI
MS.gene64324	MS.gene49271	PPI
MS.gene49271	MS.gene53650	PPI
MS.gene49271	MS.gene066863	PPI
MS.gene49271	MS.gene02545	PPI
MS.gene49271	MS.gene04915	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49271.t1	MTR_4g103630	96.429	140	5	0	1	140	1	140	1.68e-91	263
MS.gene49271.t1	MTR_4g103655	95.714	140	6	0	1	140	1	140	1.21e-90	260
MS.gene49271.t1	MTR_4g103650	97.059	136	4	0	5	140	1	136	2.84e-88	254
MS.gene49271.t1	MTR_3g089070	73.571	140	37	0	1	140	1	140	9.94e-68	202
MS.gene49271.t1	MTR_3g089090	74.453	137	35	0	4	140	3	139	2.94e-67	201
MS.gene49271.t1	MTR_6g023460	59.701	134	54	0	6	139	8	141	1.13e-53	167
MS.gene49271.t1	MTR_8g078270	54.135	133	56	2	10	139	57	187	4.79e-37	126
MS.gene49271.t1	MTR_3g067610	41.791	134	75	1	6	139	5	135	3.33e-29	104
MS.gene49271.t1	MTR_8g105230	43.182	132	74	1	6	136	36	167	4.48e-27	100
MS.gene49271.t1	MTR_8g107110	38.971	136	83	0	6	141	24	159	5.68e-27	100
MS.gene49271.t1	MTR_5g079340	38.806	134	82	0	6	139	4	137	2.19e-26	97.8
MS.gene49271.t1	MTR_4g067270	39.568	139	81	2	6	141	5	143	6.82e-26	96.7
MS.gene49271.t1	MTR_1g032070	40.876	137	80	1	6	141	46	182	8.27e-25	95.5
MS.gene49271.t1	MTR_1g046950	34.752	141	81	2	6	139	4	140	5.24e-23	89.0
MS.gene49271.t1	MTR_7g075040	38.000	150	83	3	1	140	42	191	9.74e-23	89.7
MS.gene49271.t1	MTR_1g047100	34.559	136	87	1	6	139	4	139	3.08e-22	87.0
MS.gene49271.t1	MTR_6g079570	35.971	139	88	1	6	143	26	164	8.87e-22	87.0
MS.gene49271.t1	MTR_3g083470	38.519	135	72	3	6	139	93	217	7.58e-21	85.9
MS.gene49271.t1	MTR_7g087610	35.000	140	81	3	6	139	12	147	1.61e-20	82.8
MS.gene49271.t1	MTR_4g086260	35.821	134	85	1	6	139	57	189	2.10e-20	84.0
MS.gene49271.t1	MTR_6g025320	35.714	140	80	3	6	139	12	147	2.27e-20	82.4
MS.gene49271.t1	MTR_5g088320	35.714	140	80	3	6	139	12	147	2.50e-20	82.4
MS.gene49271.t1	MTR_1g076650	34.286	140	83	2	5	139	11	146	1.65e-19	80.5
MS.gene49271.t1	MTR_2g098890	37.589	141	83	2	6	141	64	204	6.95e-19	80.1
MS.gene49271.t1	MTR_8g036075	35.417	144	84	3	6	140	49	192	9.15e-19	79.7
MS.gene49271.t1	MTR_7g089760	34.532	139	88	2	6	141	5	143	1.73e-18	77.8
MS.gene49271.t1	MTR_1g041285	33.824	136	89	1	5	139	11	146	2.97e-18	77.0
MS.gene49271.t1	MTR_5g017510	34.058	138	89	2	6	141	48	185	9.05e-18	76.6
MS.gene49271.t1	MTR_5g017560	34.058	138	89	2	6	141	48	185	9.05e-18	76.6
MS.gene49271.t1	MTR_7g090450	33.103	145	83	4	6	140	73	213	9.27e-18	77.4
MS.gene49271.t1	MTR_1g071150	32.308	130	88	0	6	135	12	141	1.61e-17	75.1
MS.gene49271.t1	MTR_8g066630	32.847	137	82	2	6	135	17	150	1.79e-17	75.5
MS.gene49271.t1	MTR_7g074240	32.667	150	88	2	3	139	21	170	2.33e-16	72.8
MS.gene49271.t1	MTR_5g017550	33.094	139	88	3	6	141	48	184	3.61e-16	72.4
MS.gene49271.t1	MTR_4g115170	33.548	155	84	3	6	141	64	218	3.83e-16	73.2
MS.gene49271.t1	MTR_5g027000	32.061	131	88	1	14	143	30	160	9.90e-16	70.9
MS.gene49271.t1	MTR_5g009830	36.220	127	76	2	15	139	380	503	4.64e-15	72.0
MS.gene49271.t1	MTR_5g008705	31.538	130	82	2	14	139	2	128	1.55e-14	67.0
MS.gene49271.t1	MTR_4g112460	29.927	137	93	1	6	139	11	147	2.89e-14	67.0
MS.gene49271.t1	MTR_5g089320	29.197	137	92	2	5	139	372	505	3.18e-14	69.7
MS.gene49271.t1	MTR_8g099095	33.858	127	79	2	15	139	380	503	5.98e-14	68.9
MS.gene49271.t1	MTR_0028s0170	29.197	137	92	2	5	139	382	515	6.64e-14	68.9
MS.gene49271.t1	MTR_3g088655	32.667	150	88	4	3	139	5	154	9.00e-14	65.5
MS.gene49271.t1	MTR_5g092810	32.576	132	84	2	10	139	401	529	1.08e-13	68.2
MS.gene49271.t1	MTR_6g007613	31.111	135	92	1	6	139	29	163	2.24e-13	64.7
MS.gene49271.t1	MTR_1g026190	28.148	135	92	2	7	139	388	519	2.62e-13	67.0
MS.gene49271.t1	MTR_0168s0120	33.043	115	67	3	31	139	1	111	4.69e-13	62.4
MS.gene49271.t1	MTR_6g025320	33.043	115	67	3	31	139	1	111	4.69e-13	62.4
MS.gene49271.t1	MTR_5g008695	33.043	115	66	2	31	138	2	112	5.13e-13	62.4
MS.gene49271.t1	MTR_4g132070	33.071	127	80	2	15	139	405	528	6.48e-13	65.9
MS.gene49271.t1	MTR_3g051770	30.882	136	88	2	10	139	392	527	6.86e-13	65.9
MS.gene49271.t1	MTR_7g074020	30.370	135	93	1	6	139	30	164	1.76e-12	62.4
MS.gene49271.t1	MTR_1g030440	30.345	145	84	3	6	139	22	160	2.91e-12	61.6
MS.gene49271.t1	MTR_4g066660	32.117	137	86	3	7	140	378	510	3.55e-12	63.9
MS.gene49271.t1	MTR_7g115040	31.884	138	93	1	6	142	12	149	1.58e-11	59.3
MS.gene49271.t1	MTR_8g069915	26.277	137	98	1	6	139	12	148	2.51e-11	59.3
MS.gene49271.t1	MTR_1g052530	31.496	127	87	0	15	141	366	492	3.79e-11	60.8
MS.gene49271.t1	MTR_7g091890	30.000	130	85	2	15	141	372	498	6.68e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49271.t1	AT1G76650	47.794	136	71	0	4	139	41	176	3.16e-36	124
MS.gene49271.t1	AT1G76650	47.794	136	71	0	4	139	41	176	3.16e-36	124
MS.gene49271.t1	AT5G42380	47.794	136	69	1	6	139	49	184	4.43e-33	116
MS.gene49271.t1	AT1G76650	46.324	136	63	1	4	139	41	166	2.50e-32	114
MS.gene49271.t1	AT1G76640	44.604	139	74	1	4	139	20	158	7.58e-32	112
MS.gene49271.t1	AT3G50770	44.755	143	73	2	3	139	61	203	8.42e-31	110
MS.gene49271.t1	AT1G24620	44.444	135	74	1	6	139	37	171	1.19e-29	107
MS.gene49271.t1	AT3G01830	44.604	139	70	4	6	140	11	146	1.46e-27	100
MS.gene49271.t1	AT1G73630	36.496	137	84	1	3	139	17	150	2.23e-25	95.9
MS.gene49271.t1	AT5G37770	35.971	139	84	1	6	139	17	155	2.89e-25	95.5
MS.gene49271.t1	AT5G37770	35.971	139	84	1	6	139	17	155	2.89e-25	95.5
MS.gene49271.t1	AT1G18210	38.129	139	83	1	1	139	18	153	4.22e-25	95.5
MS.gene49271.t1	AT1G18210	38.129	139	83	1	1	139	18	153	4.22e-25	95.5
MS.gene49271.t1	AT1G66400	33.577	137	88	1	6	139	15	151	1.07e-23	91.3
MS.gene49271.t1	AT4G03290	39.007	141	80	2	6	140	5	145	1.42e-22	88.6
MS.gene49271.t1	AT1G05990	38.571	140	82	2	6	141	5	144	3.20e-22	87.4
MS.gene49271.t1	AT4G12860	38.129	139	83	2	6	141	5	143	1.05e-21	86.3
MS.gene49271.t1	AT2G43290	35.172	145	84	3	6	141	65	208	3.22e-21	86.3
MS.gene49271.t1	AT3G59440	35.461	141	89	1	3	141	48	188	3.73e-21	85.9
MS.gene49271.t1	AT5G37780	36.429	140	79	3	6	139	12	147	1.06e-20	83.6
MS.gene49271.t1	AT1G66410	36.429	140	79	3	6	139	12	147	1.06e-20	83.6
MS.gene49271.t1	AT5G21274	36.429	140	79	3	6	139	12	147	1.24e-20	83.2
MS.gene49271.t1	AT3G56800	35.714	140	80	3	6	139	12	147	1.65e-20	82.8
MS.gene49271.t1	AT2G27030	35.714	140	80	3	6	139	12	147	1.65e-20	82.8
MS.gene49271.t1	AT2G41110	35.714	140	80	3	6	139	12	147	1.65e-20	82.8
MS.gene49271.t1	AT3G07490	37.410	139	84	2	6	141	5	143	2.09e-20	82.8
MS.gene49271.t1	AT3G43810	35.714	140	80	3	6	139	12	147	2.38e-20	82.4
MS.gene49271.t1	AT2G27030	35.714	140	80	3	6	139	12	147	4.50e-20	82.8
MS.gene49271.t1	AT3G43810	34.722	144	84	3	6	143	12	151	4.58e-20	83.6
MS.gene49271.t1	AT3G22930	34.307	137	88	2	5	139	34	170	9.01e-20	81.6
MS.gene49271.t1	AT5G17470	35.294	136	81	2	7	139	7	138	1.31e-19	80.5
MS.gene49271.t1	AT2G15680	37.778	135	81	2	6	139	50	182	1.63e-19	81.3
MS.gene49271.t1	AT4G14640	34.532	139	82	2	6	139	13	147	3.40e-18	77.0
MS.gene49271.t1	AT2G41110	32.895	152	80	4	6	139	12	159	4.85e-18	77.0
MS.gene49271.t1	AT2G36180	34.074	135	85	2	9	139	3	137	6.76e-18	75.9
MS.gene49271.t1	AT3G10190	32.143	140	93	2	3	140	67	206	7.71e-18	77.4
MS.gene49271.t1	AT2G41100	28.662	157	94	1	6	144	66	222	1.35e-17	78.2
MS.gene49271.t1	AT2G41100	28.662	157	94	1	6	144	66	222	1.35e-17	78.2
MS.gene49271.t1	AT2G41100	28.662	157	94	1	6	144	66	222	1.35e-17	78.2
MS.gene49271.t1	AT2G41100	28.662	157	94	1	6	144	101	257	2.09e-17	78.2
MS.gene49271.t1	AT2G41100	27.152	151	93	1	6	139	12	162	8.24e-14	68.2
MS.gene49271.t1	AT2G41100	28.662	157	94	1	6	144	101	257	2.09e-17	78.2
MS.gene49271.t1	AT2G41100	27.152	151	93	1	6	139	12	162	8.24e-14	68.2
MS.gene49271.t1	AT5G37780	32.903	155	79	4	6	139	12	162	6.15e-17	73.9
MS.gene49271.t1	AT3G50360	30.935	139	95	1	2	139	23	161	1.43e-15	70.5
MS.gene49271.t1	AT4G23650	34.884	129	79	2	14	140	391	516	2.91e-15	72.8
MS.gene49271.t1	AT5G37780	30.723	166	79	4	6	139	12	173	3.63e-15	69.7
MS.gene49271.t1	AT4G20780	29.814	161	92	3	5	144	28	188	7.12e-15	69.3
MS.gene49271.t1	AT5G37780	34.127	126	73	3	20	139	13	134	9.31e-15	67.8
MS.gene49271.t1	AT3G51920	31.852	135	91	1	6	139	12	146	1.53e-14	67.4
MS.gene49271.t1	AT1G66410	34.127	126	73	3	20	139	36	157	1.54e-14	67.8
MS.gene49271.t1	AT3G03400	33.088	136	82	4	3	132	2	134	2.78e-14	66.2
MS.gene49271.t1	AT5G12180	30.657	137	90	2	5	139	377	510	2.89e-14	69.7
MS.gene49271.t1	AT4G37010	28.676	136	96	1	6	140	31	166	3.38e-14	67.0
MS.gene49271.t1	AT4G37010	28.676	136	96	1	6	140	27	162	3.48e-14	67.0
MS.gene49271.t1	AT1G18530	29.197	137	94	1	6	139	7	143	4.87e-14	66.2
MS.gene49271.t1	AT4G04695	31.111	135	88	2	7	139	337	468	1.19e-13	68.2
MS.gene49271.t1	AT5G44460	29.412	153	95	3	5	144	27	179	1.99e-13	65.1
MS.gene49271.t1	AT1G66410	33.913	115	66	3	31	139	1	111	2.29e-13	63.5
MS.gene49271.t1	AT1G66410	33.913	115	66	3	31	139	1	111	2.29e-13	63.5
MS.gene49271.t1	AT2G41090	31.111	135	90	2	6	139	12	144	2.40e-13	65.1
MS.gene49271.t1	AT4G04700	31.111	135	88	2	7	139	337	468	2.50e-13	67.0
MS.gene49271.t1	AT4G14640	32.800	125	75	2	20	139	9	129	4.10e-13	63.2
MS.gene49271.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.91e-13	62.4
MS.gene49271.t1	AT3G43810	33.043	115	67	3	31	139	1	111	4.91e-13	62.4
MS.gene49271.t1	AT4G21940	31.724	145	94	2	15	157	415	556	6.34e-13	65.9
MS.gene49271.t1	AT4G21940	33.083	133	84	2	15	145	415	544	6.76e-13	65.9
MS.gene49271.t1	AT5G19360	33.071	127	80	2	15	139	382	505	9.88e-13	65.5
MS.gene49271.t1	AT1G50700	31.061	132	86	2	10	139	382	510	9.97e-13	65.5
MS.gene49271.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.27e-12	64.7
MS.gene49271.t1	AT1G76040	33.071	127	80	2	15	139	187	310	1.27e-12	64.7
MS.gene49271.t1	AT1G76040	31.111	135	88	2	7	139	417	548	1.33e-12	65.1
MS.gene49271.t1	AT3G20410	31.818	132	85	2	10	139	400	528	1.53e-12	64.7
MS.gene49271.t1	AT3G20410	31.818	132	85	2	10	139	400	528	1.53e-12	64.7
MS.gene49271.t1	AT1G62820	35.714	126	72	4	14	135	21	141	1.59e-12	62.0
MS.gene49271.t1	AT3G51850	30.952	126	87	0	15	140	368	493	1.73e-12	64.7
MS.gene49271.t1	AT2G41410	31.884	138	90	3	6	139	70	207	1.77e-12	63.2
MS.gene49271.t1	AT1G12310	35.714	126	72	4	14	135	21	141	2.39e-12	61.6
MS.gene49271.t1	AT2G31500	33.784	148	86	5	3	141	365	509	3.39e-12	63.9
MS.gene49271.t1	AT1G32250	29.286	140	86	2	6	135	16	152	4.99e-12	61.2
MS.gene49271.t1	AT1G74740	29.771	131	90	2	12	141	370	499	6.72e-12	63.2
MS.gene49271.t1	AT2G41100	28.571	140	82	1	2	141	8	129	7.70e-12	61.2
MS.gene49271.t1	AT3G01830	42.453	106	54	4	6	107	11	113	8.39e-12	60.1
MS.gene49271.t1	AT3G25600	27.737	137	96	1	6	139	12	148	8.43e-12	60.5
MS.gene49271.t1	AT4G04720	31.250	128	83	2	14	139	392	516	9.81e-12	62.4
MS.gene49271.t1	AT4G04720	31.250	128	83	2	14	139	392	516	9.81e-12	62.4
MS.gene49271.t1	AT3G03000	30.303	132	90	1	6	135	20	151	1.27e-11	60.1
MS.gene49271.t1	AT2G41100	36.232	69	44	0	73	141	7	75	5.46e-11	57.8

Find 40 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTGATTTATTTGCTTTC+TGG	0.104979	8.2:+20643518	MS.gene49271:CDS
ATTTATTTGCTTTCTGGTTT+TGG	0.144543	8.2:+20643524	MS.gene49271:CDS
GGAGGATTTAATAACGCTTA+TGG	0.268235	8.2:+20643268	MS.gene49271:CDS
AACATCCTCTTCAAACTCTT+TGG	0.271094	8.2:-20643374	None:intergenic
CATCACTTTACATTCTTCAA+TGG	0.327118	8.2:-20643420	None:intergenic
GATGATTGGAAGGTTTGATT+TGG	0.354750	8.2:+20643439	MS.gene49271:CDS
TTAATAACGCTTATGGAATC+CGG	0.394530	8.2:+20643275	MS.gene49271:CDS
AGATTTCACCGAACGAGTTA+AGG	0.395728	8.2:+20643150	MS.gene49271:CDS
AAGAAAAGTTGAAGGATTTG+AGG	0.418092	8.2:+20643306	MS.gene49271:CDS
GGAAATTGCTATTGAGGCAT+TGG	0.422915	8.2:+20643217	MS.gene49271:CDS
TGGTGATGGGCTATTGAGTT+TGG	0.423743	8.2:+20643247	MS.gene49271:CDS
AGAAAAGTTGAAGGATTTGA+GGG	0.426919	8.2:+20643307	MS.gene49271:CDS
AGGAGTCGAATGGCGAAGAT+TGG	0.437882	8.2:+20643170	MS.gene49271:CDS
ATTCGACTCCTTAACTCGTT+CGG	0.474759	8.2:-20643158	None:intergenic
GAAGAATGTAAAGTGATGAT+TGG	0.486801	8.2:+20643425	MS.gene49271:CDS
TAATAACGCTTATGGAATCC+GGG	0.494077	8.2:+20643276	MS.gene49271:CDS
AGAAGTGGAAATTGCTATTG+AGG	0.499700	8.2:+20643211	MS.gene49271:CDS
AATGTAAAGTGATGATTGGA+AGG	0.500397	8.2:+20643429	MS.gene49271:CDS
AGAGTTTCAATTGAAAGAAG+TGG	0.502556	8.2:+20643196	MS.gene49271:CDS
TGAAGAATTCAGAATCATGA+TGG	0.506151	8.2:+20643484	MS.gene49271:CDS
AGGTTTGATTTGGATGGTGA+TGG	0.507936	8.2:+20643449	MS.gene49271:CDS
ATTGGAAGGTTTGATTTGGA+TGG	0.510943	8.2:+20643443	MS.gene49271:CDS
AGTCGAATGGCGAAGATTGG+CGG	0.513078	8.2:+20643173	MS.gene49271:CDS
TGATGGGCTATTGAGTTTGG+AGG	0.520299	8.2:+20643250	MS.gene49271:CDS
TTTGAAATGTATGATAATGA+AGG	0.534288	8.2:+20643335	MS.gene49271:CDS
ATGTATGATAATGAAGGATG+TGG	0.537649	8.2:+20643341	MS.gene49271:CDS
GCATTGGATTCTGATGGTGA+TGG	0.540839	8.2:+20643233	MS.gene49271:CDS
TACTTTGATGAAGACGGTGA+TGG	0.542295	8.2:+20643125	MS.gene49271:CDS
GGGAGAAGAAGAAAAGTTGA+AGG	0.545380	8.2:+20643298	MS.gene49271:CDS
CTAAGTTACTTTGATGAAGA+CGG	0.574980	8.2:+20643119	MS.gene49271:CDS
CATTGGATTCTGATGGTGAT+GGG	0.600002	8.2:+20643234	MS.gene49271:CDS
GAATTCAGAATCATGATGGA+GGG	0.601459	8.2:+20643488	MS.gene49271:CDS
GAACGAGTTAAGGAGTCGAA+TGG	0.604236	8.2:+20643160	MS.gene49271:CDS
TAACACCAAAGAGTTTGAAG+AGG	0.608054	8.2:+20643369	MS.gene49271:CDS
AGAATTCAGAATCATGATGG+AGG	0.611144	8.2:+20643487	MS.gene49271:CDS
AATTAACATGAGGAGTAACA+TGG	0.611175	8.2:+20643082	None:intergenic
ATTGAGGCATTGGATTCTGA+TGG	0.616237	8.2:+20643227	MS.gene49271:CDS
TGTATGATAATGAAGGATGT+GGG	0.638642	8.2:+20643342	MS.gene49271:CDS
AATAACGCTTATGGAATCCG+GGG	0.638897	8.2:+20643277	MS.gene49271:CDS
ATAACGCTTATGGAATCCGG+GGG	0.690847	8.2:+20643278	MS.gene49271:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGAAATGTATGATAATGA+AGG	+	chr8.2:20643335-20643354	MS.gene49271:CDS	20.0%
!!	TCATTCTTTTGTACAATGTA+TGG	+	chr8.2:20643569-20643588	MS.gene49271:CDS	25.0%
!!!	ATGTTGATTTATTTGCTTTC+TGG	+	chr8.2:20643518-20643537	MS.gene49271:CDS	25.0%
!!!	ATTTATTTGCTTTCTGGTTT+TGG	+	chr8.2:20643524-20643543	MS.gene49271:CDS	25.0%
	AAGAAAAGTTGAAGGATTTG+AGG	+	chr8.2:20643306-20643325	MS.gene49271:CDS	30.0%
	AAGAGGATGTTGAAAAAGTT+GGG	+	chr8.2:20643386-20643405	MS.gene49271:CDS	30.0%
	AATGTAAAGTGATGATTGGA+AGG	+	chr8.2:20643429-20643448	MS.gene49271:CDS	30.0%
	AGAAAAGTTGAAGGATTTGA+GGG	+	chr8.2:20643307-20643326	MS.gene49271:CDS	30.0%
	AGAGTTTCAATTGAAAGAAG+TGG	+	chr8.2:20643196-20643215	MS.gene49271:CDS	30.0%
	CATCACTTTACATTCTTCAA+TGG	-	chr8.2:20643423-20643442	None:intergenic	30.0%
	GAAGAATGTAAAGTGATGAT+TGG	+	chr8.2:20643425-20643444	MS.gene49271:CDS	30.0%
	TGAAGAATTCAGAATCATGA+TGG	+	chr8.2:20643484-20643503	MS.gene49271:CDS	30.0%
	TTAATAACGCTTATGGAATC+CGG	+	chr8.2:20643275-20643294	MS.gene49271:CDS	30.0%
!	ATGTATGATAATGAAGGATG+TGG	+	chr8.2:20643341-20643360	MS.gene49271:CDS	30.0%
!	CTAAGTTACTTTGATGAAGA+CGG	+	chr8.2:20643119-20643138	MS.gene49271:CDS	30.0%
!	TGTATGATAATGAAGGATGT+GGG	+	chr8.2:20643342-20643361	MS.gene49271:CDS	30.0%
	AACATCCTCTTCAAACTCTT+TGG	-	chr8.2:20643377-20643396	None:intergenic	35.0%
	AGAATTCAGAATCATGATGG+AGG	+	chr8.2:20643487-20643506	MS.gene49271:CDS	35.0%
	AGAGGATGTTGAAAAAGTTG+GGG	+	chr8.2:20643387-20643406	MS.gene49271:CDS	35.0%
	GAAGAGGATGTTGAAAAAGT+TGG	+	chr8.2:20643385-20643404	MS.gene49271:CDS	35.0%
	GATGATTGGAAGGTTTGATT+TGG	+	chr8.2:20643439-20643458	MS.gene49271:CDS	35.0%
	GGAGGATTTAATAACGCTTA+TGG	+	chr8.2:20643268-20643287	MS.gene49271:CDS	35.0%
	TAACACCAAAGAGTTTGAAG+AGG	+	chr8.2:20643369-20643388	MS.gene49271:CDS	35.0%
	TAATAACGCTTATGGAATCC+GGG	+	chr8.2:20643276-20643295	MS.gene49271:CDS	35.0%
!	AGAAGTGGAAATTGCTATTG+AGG	+	chr8.2:20643211-20643230	MS.gene49271:CDS	35.0%
!	GAATTCAGAATCATGATGGA+GGG	+	chr8.2:20643488-20643507	MS.gene49271:CDS	35.0%
!!	ATTGGAAGGTTTGATTTGGA+TGG	+	chr8.2:20643443-20643462	MS.gene49271:CDS	35.0%
	AATAACGCTTATGGAATCCG+GGG	+	chr8.2:20643277-20643296	MS.gene49271:CDS	40.0%
	AGATTTCACCGAACGAGTTA+AGG	+	chr8.2:20643150-20643169	MS.gene49271:CDS	40.0%
	ATTCGACTCCTTAACTCGTT+CGG	-	chr8.2:20643161-20643180	None:intergenic	40.0%
	GAGGATGTTGAAAAAGTTGG+GGG	+	chr8.2:20643388-20643407	MS.gene49271:CDS	40.0%
	GGGAGAAGAAGAAAAGTTGA+AGG	+	chr8.2:20643298-20643317	MS.gene49271:CDS	40.0%
	TACTTTGATGAAGACGGTGA+TGG	+	chr8.2:20643125-20643144	MS.gene49271:CDS	40.0%
!	CATTGGATTCTGATGGTGAT+GGG	+	chr8.2:20643234-20643253	MS.gene49271:CDS	40.0%
!	GGAAATTGCTATTGAGGCAT+TGG	+	chr8.2:20643217-20643236	MS.gene49271:CDS	40.0%
!!	AGGTTTGATTTGGATGGTGA+TGG	+	chr8.2:20643449-20643468	MS.gene49271:CDS	40.0%
!!	ATTGAGGCATTGGATTCTGA+TGG	+	chr8.2:20643227-20643246	MS.gene49271:CDS	40.0%
	ATAACGCTTATGGAATCCGG+GGG	+	chr8.2:20643278-20643297	MS.gene49271:CDS	45.0%
	GAACGAGTTAAGGAGTCGAA+TGG	+	chr8.2:20643160-20643179	MS.gene49271:CDS	45.0%
!	CAACTTTTCTTCTTCTCCCC+CGG	-	chr8.2:20643297-20643316	None:intergenic	45.0%
!	GCATTGGATTCTGATGGTGA+TGG	+	chr8.2:20643233-20643252	MS.gene49271:CDS	45.0%
!!	TGATGGGCTATTGAGTTTGG+AGG	+	chr8.2:20643250-20643269	MS.gene49271:CDS	45.0%
!!	TGGTGATGGGCTATTGAGTT+TGG	+	chr8.2:20643247-20643266	MS.gene49271:CDS	45.0%
	AGAATGAATGAAACCCCCGC+AGG	-	chr8.2:20643556-20643575	None:intergenic	50.0%
	AGGAGTCGAATGGCGAAGAT+TGG	+	chr8.2:20643170-20643189	MS.gene49271:CDS	50.0%
	AGTCGAATGGCGAAGATTGG+CGG	+	chr8.2:20643173-20643192	MS.gene49271:CDS	50.0%
	AATGAAACCCCCGCAGGCAC+TGG	-	chr8.2:20643550-20643569	None:intergenic	60.0%
!!!	CTGGTTTTGGCCAGTGCCTG+CGG	+	chr8.2:20643537-20643556	MS.gene49271:CDS	60.0%
!!!	TGGTTTTGGCCAGTGCCTGC+GGG	+	chr8.2:20643538-20643557	MS.gene49271:CDS	60.0%
!!	GGTTTTGGCCAGTGCCTGCG+GGG	+	chr8.2:20643539-20643558	MS.gene49271:CDS	65.0%
!!	GTTTTGGCCAGTGCCTGCGG+GGG	+	chr8.2:20643540-20643559	MS.gene49271:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	20643089	20643595	20643089	ID=MS.gene49271
chr8.2	mRNA	20643089	20643595	20643089	ID=MS.gene49271.t1;Parent=MS.gene49271
chr8.2	exon	20643089	20643595	20643089	ID=MS.gene49271.t1.exon1;Parent=MS.gene49271.t1
chr8.2	CDS	20643089	20643595	20643089	ID=cds.MS.gene49271.t1;Parent=MS.gene49271.t1

Gene Sequence

>MS.gene49271

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGGCGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGTATGATAATGAAGGATGTGGGTTCATAACACCAAAGAGTTTGAAGAGGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGGGAGTGATAATGTTGATTTATTTGCTTTCTGGTTTTGGCCAGTGCCTGCGGGGGTTTCATTCATTCTTTTGTACAATGTATGGCTAA

Protein sequence

>MS.gene49271.t1

MRSNMEFERVLSYFDEDGDGKISPNELRSRMAKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMYDNEGCGFITPKSLKRMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMMEGVIMLIYLLSGFGQCLRGFHSFFCTMYG