AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53150.t1 MTR_5g043580 98.295 176 3 0 1 176 1 176 1.92e-128 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53150.t1 AT2G03420 73.585 106 28 0 69 174 63 168 1.00e-53 168

Find 36 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTACGAGGGGCGGAAT+GGG 0.329826 5.4:-32806750 MS.gene53150:CDS
CTTCTATTGCAATTCTTCTC+TGG 0.348974 5.4:-32808124 MS.gene53150:CDS
ATGAATTTGTCCCTTGCCTC+TGG 0.354711 5.4:+32806695 None:intergenic
CCTCAAAAGAAGGTTCAGTT+TGG 0.387035 5.4:-32808233 MS.gene53150:CDS
TGAAACCATTAAAACGTTTG+AGG 0.439460 5.4:+32807584 None:intergenic
AAGTCAATCTGAGGCAGCTC+AGG 0.441780 5.4:+32807607 None:intergenic
GTAAGAATGATGAGAAGATA+AGG 0.480601 5.4:+32808178 None:intergenic
CGAGGGGCGGAATGGGGAGT+TGG 0.490686 5.4:-32806743 MS.gene53150:CDS
GCTCAAGCAGTTTCTTCACC+AGG 0.491458 5.4:+32807416 None:intergenic
ACTGCTTACGAGGGGCGGAA+TGG 0.499290 5.4:-32806751 MS.gene53150:CDS
CATAGCAATGTTGAAGGAGA+AGG 0.511435 5.4:-32806675 MS.gene53150:CDS
GCACATGCTGGATTCATGTC+AGG 0.519014 5.4:-32807658 MS.gene53150:CDS
CAAATTCATAGCAATGTTGA+AGG 0.527626 5.4:-32806681 MS.gene53150:CDS
ATTCTTCTCTGGTCAACTCC+AGG 0.534134 5.4:-32808113 MS.gene53150:intron
CTCAAGCAGTTTCTTCACCA+GGG 0.535692 5.4:+32807417 None:intergenic
ATCTGAGGCAGCTCAGGACC+AGG 0.536804 5.4:+32807613 None:intergenic
ACTGAGCAATCCTCAAAAGA+AGG 0.537649 5.4:-32808243 MS.gene53150:CDS
GTGGAAATCTGAATGAAAGA+TGG 0.538239 5.4:+32808278 None:intergenic
GGGAATGACTCCAGAGGCAA+GGG 0.550474 5.4:-32806705 MS.gene53150:CDS
GGATTCATGTCAGGAATCAC+AGG 0.551319 5.4:-32807649 MS.gene53150:CDS
TCAAGCAGTTTCTTCACCAG+GGG 0.567433 5.4:+32807418 None:intergenic
TGCTTACGAGGGGCGGAATG+GGG 0.568619 5.4:-32806749 MS.gene53150:CDS
AATGAAAGATGGTAATGAAG+AGG 0.576236 5.4:+32808289 None:intergenic
AGGGAATGACTCCAGAGGCA+AGG 0.577514 5.4:-32806706 MS.gene53150:CDS
GAGAACAAGTACTGCTTACG+AGG 0.584541 5.4:-32806761 MS.gene53150:CDS
GTTGGTGATTGTTCAGCAGA+GGG 0.594398 5.4:-32806725 MS.gene53150:CDS
AGTTGGTGATTGTTCAGCAG+AGG 0.606424 5.4:-32806726 MS.gene53150:CDS
CAAGTACTGCTTACGAGGGG+CGG 0.612792 5.4:-32806756 MS.gene53150:CDS
ACAGGACTAGAGTCTGTACC+TGG 0.626927 5.4:-32807631 MS.gene53150:CDS
GCAATGTTGAAGGAGAAGGT+TGG 0.630694 5.4:-32806671 MS.gene53150:CDS
CGTTTGAGGAAGTCAATCTG+AGG 0.656409 5.4:+32807598 None:intergenic
AGAACAAGTACTGCTTACGA+GGG 0.671236 5.4:-32806760 MS.gene53150:CDS
ATTGCAATAGAAGCAGCCAA+AGG 0.674848 5.4:+32808134 None:intergenic
AAGATTTAAAGAGACACCCC+TGG 0.680049 5.4:-32807434 MS.gene53150:CDS
AGCAGAGGGAATGACTCCAG+AGG 0.703314 5.4:-32806711 MS.gene53150:CDS
GAACAAGTACTGCTTACGAG+GGG 0.743478 5.4:-32806759 MS.gene53150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATCATATAAGTAACT+AGG - chr5.4:32807867-32807886 MS.gene53150:intron 15.0%
!! TAATGTCCAATTAAAAAAAT+TGG - chr5.4:32807756-32807775 MS.gene53150:intron 15.0%
!! TTTGAATGGTTAATATATTT+CGG - chr5.4:32808006-32808025 MS.gene53150:intron 15.0%
!! AGTTTCATTTCATGAAATTT+AGG + chr5.4:32807436-32807455 None:intergenic 20.0%
!! ATTGACAAAAATGTAAGAAT+TGG - chr5.4:32807920-32807939 MS.gene53150:intron 20.0%
!! CTTTAATTCTTAAGAATCAT+AGG + chr5.4:32807180-32807199 None:intergenic 20.0%
! AAATTGTGTATGAGAAATGT+TGG + chr5.4:32807715-32807734 None:intergenic 25.0%
! AACATTTCTCATACACAATT+TGG - chr5.4:32807714-32807733 MS.gene53150:intron 25.0%
! ACCATTCAAAAATAAAGTCA+TGG + chr5.4:32807996-32808015 None:intergenic 25.0%
! CTCACAAAAAAGAATATTAC+AGG + chr5.4:32807959-32807978 None:intergenic 25.0%
! TAGCCTAAAAAAATGATAAG+AGG + chr5.4:32807842-32807861 None:intergenic 25.0%
! TCCAATTAAAAAAATTGGCT+CGG - chr5.4:32807761-32807780 MS.gene53150:intron 25.0%
! TTAATACCGAGACAAATAAT+AGG + chr5.4:32808086-32808105 None:intergenic 25.0%
! TTAGATCCTATTATTTGTCT+CGG - chr5.4:32808077-32808096 MS.gene53150:intron 25.0%
!! AAGATAGTTTGAGTTAGTTA+GGG - chr5.4:32807056-32807075 MS.gene53150:intron 25.0%
!! TAAGATAGTTTGAGTTAGTT+AGG - chr5.4:32807055-32807074 MS.gene53150:intron 25.0%
!! TTTTGTCAATATAAGAAGAC+AGG + chr5.4:32807910-32807929 None:intergenic 25.0%
!!! ACCATGACTTTATTTTTGAA+TGG - chr5.4:32807992-32808011 MS.gene53150:intron 25.0%
ACATATCAACATAGCACTTT+AGG + chr5.4:32807675-32807694 None:intergenic 30.0%
ATATGAGATAGAGATAGCAT+AGG + chr5.4:32807133-32807152 None:intergenic 30.0%
CAAATTCATAGCAATGTTGA+AGG - chr5.4:32808276-32808295 MS.gene53150:CDS 30.0%
CACAACATGTTATAAGAGTT+TGG + chr5.4:32807403-32807422 None:intergenic 30.0%
CATATCAACATAGCACTTTA+GGG + chr5.4:32807674-32807693 None:intergenic 30.0%
GTAAGAATGATGAGAAGATA+AGG + chr5.4:32806782-32806801 None:intergenic 30.0%
TCTTCAAAAATCAGTTCCTT+TGG - chr5.4:32806807-32806826 MS.gene53150:intron 30.0%
TGAAACCATTAAAACGTTTG+AGG + chr5.4:32807376-32807395 None:intergenic 30.0%
! AAAGTGCTATGTTGATATGT+CGG - chr5.4:32807675-32807694 MS.gene53150:intron 30.0%
! AATGAAAGATGGTAATGAAG+AGG + chr5.4:32806671-32806690 None:intergenic 30.0%
! CATTTTCAGCATATTTCTTC+TGG + chr5.4:32807499-32807518 None:intergenic 30.0%
!!! GAGCCTCTTATCATTTTTTT+AGG - chr5.4:32807836-32807855 MS.gene53150:intron 30.0%
!!! TCCGAGCCAATTTTTTTAAT+TGG + chr5.4:32807765-32807784 None:intergenic 30.0%
ATAAGAGGCTCAATAGATGT+TGG + chr5.4:32807827-32807846 None:intergenic 35.0%
ATCTTTGTGCTTTACTCTTC+AGG - chr5.4:32808158-32808177 MS.gene53150:CDS 35.0%
CTGCATTAAAGCATGGTAAA+AGG + chr5.4:32806878-32806897 None:intergenic 35.0%
CTTCTATTGCAATTCTTCTC+TGG - chr5.4:32806833-32806852 MS.gene53150:intron 35.0%
GTGGAAATCTGAATGAAAGA+TGG + chr5.4:32806682-32806701 None:intergenic 35.0%
TGACGATTATGATTGATCGA+CGG - chr5.4:32807219-32807238 MS.gene53150:intron 35.0%
TTCATTAGAGACACATGCTA+AGG - chr5.4:32806920-32806939 MS.gene53150:intron 35.0%
! ATTGCTCAGTTTTTCACAGA+AGG + chr5.4:32806704-32806723 None:intergenic 35.0%
! GACTTCCTCAAACGTTTTAA+TGG - chr5.4:32807368-32807387 MS.gene53150:intron 35.0%
! TGGTAAAAGGTTACTTTACC+TGG + chr5.4:32806865-32806884 None:intergenic 35.0%
! TTGATATGTCGGATACGTTT+TGG - chr5.4:32807686-32807705 MS.gene53150:intron 35.0%
!! TGAGTTAGTTAGGGTTAGTT+AGG - chr5.4:32807065-32807084 MS.gene53150:intron 35.0%
AAGATTTAAAGAGACACCCC+TGG - chr5.4:32807523-32807542 MS.gene53150:intron 40.0%
ACTGAGCAATCCTCAAAAGA+AGG - chr5.4:32806714-32806733 MS.gene53150:CDS 40.0%
ATAGGATCTAATGCACACAC+CGG + chr5.4:32808068-32808087 None:intergenic 40.0%
ATTGCAATAGAAGCAGCCAA+AGG + chr5.4:32806826-32806845 None:intergenic 40.0%
CATAGCAATGTTGAAGGAGA+AGG - chr5.4:32808282-32808301 MS.gene53150:CDS 40.0%
CCTCAAAAGAAGGTTCAGTT+TGG - chr5.4:32806724-32806743 MS.gene53150:CDS 40.0%
TTGCATGCTGCATTAAAGCA+TGG + chr5.4:32806885-32806904 None:intergenic 40.0%
! AGAACAAGTACTGCTTACGA+GGG - chr5.4:32808197-32808216 MS.gene53150:CDS 40.0%
! CCAAACTGAACCTTCTTTTG+AGG + chr5.4:32806727-32806746 None:intergenic 40.0%
!!! TCTTATTTTGCAGCACATGC+TGG - chr5.4:32807287-32807306 MS.gene53150:intron 40.0%
ATCTAATGCACACACCGGAT+AGG + chr5.4:32808063-32808082 None:intergenic 45.0%
ATGAATTTGTCCCTTGCCTC+TGG + chr5.4:32808265-32808284 None:intergenic 45.0%
ATTCTTCTCTGGTCAACTCC+AGG - chr5.4:32806844-32806863 MS.gene53150:intron 45.0%
CGTTTGAGGAAGTCAATCTG+AGG + chr5.4:32807362-32807381 None:intergenic 45.0%
CTCAAGCAGTTTCTTCACCA+GGG + chr5.4:32807543-32807562 None:intergenic 45.0%
GCAATGTTGAAGGAGAAGGT+TGG - chr5.4:32808286-32808305 MS.gene53150:CDS 45.0%
GGATTCATGTCAGGAATCAC+AGG - chr5.4:32807308-32807327 MS.gene53150:intron 45.0%
GTGTCTCTCTTTGTCCTATC+CGG - chr5.4:32808046-32808065 MS.gene53150:intron 45.0%
TCAAGCAGTTTCTTCACCAG+GGG + chr5.4:32807542-32807561 None:intergenic 45.0%
! AGGCTCAATAGATGTTGGCT+CGG + chr5.4:32807822-32807841 None:intergenic 45.0%
! CAACATAGCACTTTAGGGAG+AGG + chr5.4:32807669-32807688 None:intergenic 45.0%
! GAACAAGTACTGCTTACGAG+GGG - chr5.4:32808198-32808217 MS.gene53150:CDS 45.0%
! GAGAACAAGTACTGCTTACG+AGG - chr5.4:32808196-32808215 MS.gene53150:CDS 45.0%
! GCTCAGTTTTTCACAGAAGG+TGG + chr5.4:32806701-32806720 None:intergenic 45.0%
!! AAAATTGGCTCGGACCTGTT+AGG - chr5.4:32807771-32807790 MS.gene53150:intron 45.0%
!! AGTTGGTGATTGTTCAGCAG+AGG - chr5.4:32808231-32808250 MS.gene53150:CDS 45.0%
!! GTTGGTGATTGTTCAGCAGA+GGG - chr5.4:32808232-32808251 MS.gene53150:CDS 45.0%
ACAGGACTAGAGTCTGTACC+TGG - chr5.4:32807326-32807345 MS.gene53150:intron 50.0%
CAAAGAGAGACACCGCAACA+CGG + chr5.4:32808039-32808058 None:intergenic 50.0%
GCACATGCTGGATTCATGTC+AGG - chr5.4:32807299-32807318 MS.gene53150:intron 50.0%
GCTCAAGCAGTTTCTTCACC+AGG + chr5.4:32807544-32807563 None:intergenic 50.0%
GTGTCCAAGACATGCCTAAC+AGG + chr5.4:32807788-32807807 None:intergenic 50.0%
! AAGTCAATCTGAGGCAGCTC+AGG + chr5.4:32807353-32807372 None:intergenic 50.0%
AGCAGAGGGAATGACTCCAG+AGG - chr5.4:32808246-32808265 MS.gene53150:CDS 55.0%
AGGGAATGACTCCAGAGGCA+AGG - chr5.4:32808251-32808270 MS.gene53150:CDS 55.0%
CGGACCTGTTAGGCATGTCT+TGG - chr5.4:32807781-32807800 MS.gene53150:intron 55.0%
GGGAATGACTCCAGAGGCAA+GGG - chr5.4:32808252-32808271 MS.gene53150:CDS 55.0%
GGTGTCTGTGCTGTGCTCAT+AGG + chr5.4:32807648-32807667 None:intergenic 55.0%
TTCGGACACAAGCCGTGTTG+CGG - chr5.4:32808024-32808043 MS.gene53150:intron 55.0%
! CAAGTACTGCTTACGAGGGG+CGG - chr5.4:32808201-32808220 MS.gene53150:CDS 55.0%
ACTGCTTACGAGGGGCGGAA+TGG - chr5.4:32808206-32808225 MS.gene53150:CDS 60.0%
CTGCTTACGAGGGGCGGAAT+GGG - chr5.4:32808207-32808226 MS.gene53150:CDS 60.0%
TGCTTACGAGGGGCGGAATG+GGG - chr5.4:32808208-32808227 MS.gene53150:CDS 60.0%
! ATCTGAGGCAGCTCAGGACC+AGG + chr5.4:32807347-32807366 None:intergenic 60.0%
CGAGGGGCGGAATGGGGAGT+TGG - chr5.4:32808214-32808233 MS.gene53150:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 32806661 32808318 32806661 ID=MS.gene53150
chr5.4 mRNA 32806661 32808318 32806661 ID=MS.gene53150.t1;Parent=MS.gene53150
chr5.4 exon 32808114 32808318 32808114 ID=MS.gene53150.t1.exon1;Parent=MS.gene53150.t1
chr5.4 CDS 32808114 32808318 32808114 ID=cds.MS.gene53150.t1;Parent=MS.gene53150.t1
chr5.4 exon 32807590 32807679 32807590 ID=MS.gene53150.t1.exon2;Parent=MS.gene53150.t1
chr5.4 CDS 32807590 32807679 32807590 ID=cds.MS.gene53150.t1;Parent=MS.gene53150.t1
chr5.4 exon 32807394 32807490 32807394 ID=MS.gene53150.t1.exon3;Parent=MS.gene53150.t1
chr5.4 CDS 32807394 32807490 32807394 ID=cds.MS.gene53150.t1;Parent=MS.gene53150.t1
chr5.4 exon 32806661 32806799 32806661 ID=MS.gene53150.t1.exon4;Parent=MS.gene53150.t1
chr5.4 CDS 32806661 32806799 32806661 ID=cds.MS.gene53150.t1;Parent=MS.gene53150.t1
Gene Sequence

>MS.gene53150

ATGGGTTCCTCTTCATTACCATCTTTCATTCAGATTTCCACCTTCTGTGAAAAACTGAGCAATCCTCAAAAGAAGGTTCAGTTTGGAATAACAAAGAATGACACAAAATTTTCTTGCTCCTTATCTTCTCATCATTCTTACAATATTCTTCAAAAATCAGTTCCTTTGGCTGCTTCTATTGCAATTCTTCTCTGGTCAACTCCAGGTAAAGTAACCTTTTACCATGCTTTAATGCAGCATGCAATTCAAGTCACTAAACTTCATTAGAGACACATGCTAAGGCATTTTTGTTATTGAGTTTAATATTATATACTGACAAAGTAAAATAGTTTTACAGTGTTATCAAATCATAATCAATCATATATATGATTTTACAAGTAGTTATGATTGATGTTAAGATAGTTTGAGTTAGTTAGGGTTAGTTAGGATTAGTTACTCGTTAGCGTAGTTGAGTTCTATAAATAGTATTCCTATGCTATCTCTATCTCATATGAGTATGATCTGCAGCACTATGATCCTATGATTCTTAAGAATTAAAGTGAAACATTTTTAATAATCTGACGATTATGATTGATCGACGGTGTAAAAAATATTTACTGTGACAATGTATATGAATTGAATTTTTTTCTTATTTTGCAGCACATGCTGGATTCATGTCAGGAATCACAGGACTAGAGTCTGTACCTGGTCCTGAGCTGCCTCAGATTGACTTCCTCAAACGTTTTAATGGTTTCAACTTCCAAACTCTTATAACATGTTGTGATTTTGTAAACCTAAATTTCATGAAATGAAACTAACATATATTGTTTTATGATTATTATTTCACAGAAGAAAACCAGAAGAAATATGCTGAAAATGATGCAAGATTTAAAGAGACACCCCTGGTGAAGAAACTGCTTGAGCAATCTAAGTTGAATAAAGAGAAGTAATTTTTCTGTTATCATAATTGAACTGTGTATTTTTTATTTAGACAAAGTGCTATGTCCTATGAGCACAGCACAGACACCTCTCCCTAAAGTGCTATGTTGATATGTCGGATACGTTTTGGACGCCAACATTTCTCATACACAATTTGGACATGTGCTAGACACTTCTTAATGTCCAATTAAAAAAATTGGCTCGGACCTGTTAGGCATGTCTTGGACACACGTCAAGAGACCGAGCCAACATCTATTGAGCCTCTTATCATTTTTTTAGGCTATTATCAATTAATCATATAAGTAACTAGGTTTTATAAAACACACTACCTGTCTTCTTATATTGACAAAAATGTAAGAATTGGATAGTAAATGATTCCTGTAATATTCTTTTTTGTGAGAATCTCTGTCATTACCATGACTTTATTTTTGAATGGTTAATATATTTCGGACACAAGCCGTGTTGCGGTGTCTCTCTTTGTCCTATCCGGTGTGTGCATTAGATCCTATTATTTGTCTCGGTATTAACTTTGTTTAAAACATTGTTTCTATGCATCTGTTATCAGAAAGTTGCAATAACATCTTTGTGCTTTACTCTTCAGGAATAGTAAAGAAATTGAGAACAAGTACTGCTTACGAGGGGCGGAATGGGGAGTTGGTGATTGTTCAGCAGAGGGAATGACTCCAGAGGCAAGGGACAAATTCATAGCAATGTTGAAGGAGAAGGTTGGAGAAAAGTAA

Protein sequence

>MS.gene53150.t1

MGSSSLPSFIQISTFCEKLSNPQKKVQFGITKNDTKFSCSLSSHHSYNILQKSVPLAASIAILLWSTPAHAGFMSGITGLESVPGPELPQIDFLKRFNEENQKKYAENDARFKETPLVKKLLEQSKLNKEKNSKEIENKYCLRGAEWGVGDCSAEGMTPEARDKFIAMLKEKVGEK