AlfalfaGEDB | MS.gene62540

M. sativa cultivar XinJiangDaYe / MS.gene62540

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62540.t1	RHN45038.1	98.7	77	1	0	13	89	1	77	1.20E-33	152.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62540.t1	Q8RYJ9	50.8	63	31	0	18	80	6	68	2.1e-10	67.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62540.t1	A0A396GX56	98.7	77	1	0	13	89	1	77	8.6e-34	152.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060446	MS.gene62540	0.999297	1.90E-301	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21155	MS.gene62540	PPI
MS.gene62540	MS.gene93763	PPI
MS.gene21932	MS.gene62540	PPI
MS.gene28338	MS.gene62540	PPI
MS.gene008225	MS.gene62540	PPI
MS.gene056383	MS.gene62540	PPI
MS.gene64324	MS.gene62540	PPI
MS.gene015470	MS.gene62540	PPI
MS.gene62540	MS.gene87673	PPI
MS.gene63000	MS.gene62540	PPI
MS.gene62540	MS.gene53659	PPI
MS.gene22553	MS.gene62540	PPI
MS.gene53662	MS.gene62540	PPI
MS.gene80011	MS.gene62540	PPI
MS.gene62540	MS.gene061768	PPI
MS.gene62540	MS.gene89216	PPI
MS.gene070168	MS.gene62540	PPI
MS.gene62540	MS.gene64308	PPI
MS.gene62540	MS.gene009021	PPI
MS.gene62540	MS.gene47195	PPI
MS.gene62540	MS.gene017446	PPI
MS.gene62540	MS.gene96914	PPI
MS.gene62540	MS.gene066862	PPI
MS.gene62540	MS.gene96913	PPI
MS.gene038456	MS.gene62540	PPI
MS.gene62540	MS.gene07422	PPI
MS.gene004899	MS.gene62540	PPI
MS.gene62540	MS.gene64316	PPI
MS.gene62540	MS.gene80012	PPI
MS.gene62540	MS.gene061261	PPI
MS.gene070167	MS.gene62540	PPI
MS.gene62540	MS.gene026618	PPI
MS.gene05284	MS.gene62540	PPI
MS.gene62540	MS.gene53648	PPI
MS.gene041795	MS.gene62540	PPI
MS.gene62540	MS.gene008831	PPI
MS.gene62540	MS.gene55545	PPI
MS.gene62540	MS.gene056461	PPI
MS.gene62540	MS.gene98972	PPI
MS.gene62540	MS.gene64310	PPI
MS.gene62540	MS.gene29459	PPI
MS.gene62540	MS.gene71207	PPI
MS.gene63281	MS.gene62540	PPI
MS.gene68345	MS.gene62540	PPI
MS.gene62540	MS.gene92775	PPI
MS.gene001246	MS.gene62540	PPI
MS.gene040127	MS.gene62540	PPI
MS.gene62258	MS.gene62540	PPI
MS.gene62540	MS.gene63281	PPI
MS.gene000724	MS.gene62540	PPI
MS.gene052672	MS.gene62540	PPI
MS.gene64311	MS.gene62540	PPI
MS.gene62540	MS.gene21696	PPI
MS.gene25186	MS.gene62540	PPI
MS.gene62540	MS.gene074045	PPI
MS.gene62540	MS.gene03228	PPI
MS.gene58906	MS.gene62540	PPI
MS.gene020515	MS.gene62540	PPI
MS.gene62540	MS.gene46237	PPI
MS.gene62540	MS.gene64317	PPI
MS.gene58860	MS.gene62540	PPI
MS.gene27516	MS.gene62540	PPI
MS.gene053319	MS.gene62540	PPI
MS.gene04915	MS.gene62540	PPI
MS.gene61009	MS.gene62540	PPI
MS.gene64327	MS.gene62540	PPI
MS.gene64307	MS.gene62540	PPI
MS.gene62540	MS.gene93087	PPI
MS.gene62540	MS.gene046988	PPI
MS.gene04917	MS.gene62540	PPI
MS.gene62540	MS.gene057293	PPI
MS.gene026530	MS.gene62540	PPI
MS.gene62540	MS.gene09934	PPI
MS.gene036930	MS.gene62540	PPI
MS.gene62540	MS.gene79028	PPI
MS.gene013748	MS.gene62540	PPI
MS.gene62540	MS.gene026940	PPI
MS.gene62540	MS.gene65954	PPI
MS.gene62540	MS.gene85596	PPI
MS.gene62540	MS.gene96922	PPI
MS.gene62540	MS.gene80013	PPI
MS.gene015658	MS.gene62540	PPI
MS.gene62540	MS.gene025797	PPI
MS.gene024717	MS.gene62540	PPI
MS.gene62540	MS.gene21931	PPI
MS.gene62540	MS.gene64314	PPI
MS.gene62540	MS.gene09960	PPI
MS.gene64313	MS.gene62540	PPI
MS.gene83068	MS.gene62540	PPI
MS.gene62540	MS.gene94551	PPI
MS.gene62689	MS.gene62540	PPI
MS.gene97948	MS.gene62540	PPI
MS.gene003880	MS.gene62540	PPI
MS.gene54637	MS.gene62540	PPI
MS.gene000738	MS.gene62540	PPI
MS.gene67281	MS.gene62540	PPI
MS.gene025777	MS.gene62540	PPI
MS.gene62540	MS.gene53650	PPI
MS.gene062239	MS.gene62540	PPI
MS.gene62540	MS.gene066863	PPI
MS.gene46886	MS.gene62540	PPI
MS.gene62540	MS.gene02545	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62540.t1	MTR_6g023460	77.465	142	31	1	12	153	2	142	1.33e-76	224
MS.gene62540.t1	MTR_4g103655	59.259	135	54	1	18	152	6	139	2.73e-51	160
MS.gene62540.t1	MTR_4g103650	58.519	135	55	1	18	152	2	135	1.43e-50	158
MS.gene62540.t1	MTR_4g103630	57.037	135	57	1	18	152	6	139	2.24e-49	155
MS.gene62540.t1	MTR_3g089070	53.571	140	64	1	13	152	1	139	2.16e-46	148
MS.gene62540.t1	MTR_3g089090	53.623	138	63	1	15	152	2	138	3.58e-46	147
MS.gene62540.t1	MTR_8g078270	48.485	132	66	2	22	152	57	187	5.68e-35	120
MS.gene62540.t1	MTR_1g046950	39.437	142	74	3	18	152	4	140	7.82e-30	105
MS.gene62540.t1	MTR_5g079340	37.778	135	83	1	18	152	4	137	1.77e-28	102
MS.gene62540.t1	MTR_3g067610	38.519	135	79	2	18	152	5	135	1.87e-28	102
MS.gene62540.t1	MTR_1g047100	38.686	137	75	3	21	152	7	139	3.88e-27	99.4
MS.gene62540.t1	MTR_8g107110	38.519	135	82	1	18	152	24	157	7.04e-27	99.4
MS.gene62540.t1	MTR_8g105230	37.778	135	82	2	16	149	34	167	1.26e-25	96.7
MS.gene62540.t1	MTR_1g032070	38.519	135	81	2	20	153	48	181	2.90e-25	95.9
MS.gene62540.t1	MTR_6g079570	38.519	135	83	0	18	152	26	160	5.55e-25	94.7
MS.gene62540.t1	MTR_7g075040	38.255	149	83	4	13	152	42	190	2.28e-24	93.6
MS.gene62540.t1	MTR_7g087610	34.043	141	90	3	14	152	8	147	3.57e-23	89.4
MS.gene62540.t1	MTR_4g067270	35.971	139	85	3	17	152	4	141	3.67e-23	89.4
MS.gene62540.t1	MTR_5g017510	35.252	139	90	0	15	153	45	183	1.07e-22	89.0
MS.gene62540.t1	MTR_5g017560	35.252	139	90	0	15	153	45	183	1.07e-22	89.0
MS.gene62540.t1	MTR_1g076650	31.915	141	94	2	14	153	8	147	2.52e-22	87.0
MS.gene62540.t1	MTR_1g076650	46.269	67	36	0	86	152	7	73	1.46e-12	61.6
MS.gene62540.t1	MTR_5g017550	34.532	139	90	1	15	153	45	182	2.95e-22	87.8
MS.gene62540.t1	MTR_8g066630	33.813	139	87	2	14	148	13	150	3.27e-22	87.0
MS.gene62540.t1	MTR_8g036075	36.111	144	82	4	18	152	49	191	5.29e-22	87.4
MS.gene62540.t1	MTR_6g025320	33.333	141	91	3	14	152	8	147	6.91e-22	85.9
MS.gene62540.t1	MTR_6g025320	41.791	67	39	0	86	152	7	73	2.68e-11	58.5
MS.gene62540.t1	MTR_1g041285	33.333	141	92	2	14	153	8	147	2.10e-21	84.7
MS.gene62540.t1	MTR_5g088320	32.624	141	92	3	14	152	8	147	3.18e-21	84.3
MS.gene62540.t1	MTR_5g088320	43.284	67	38	0	86	152	7	73	7.08e-12	60.1
MS.gene62540.t1	MTR_4g086260	35.338	133	80	3	22	152	61	189	1.33e-20	84.0
MS.gene62540.t1	MTR_1g071150	31.206	141	95	2	14	153	8	147	4.32e-20	81.3
MS.gene62540.t1	MTR_3g083470	31.159	138	83	2	17	153	92	218	9.38e-20	82.4
MS.gene62540.t1	MTR_4g115170	36.943	157	73	5	18	152	64	216	9.72e-19	79.7
MS.gene62540.t1	MTR_7g089760	36.296	135	82	3	21	152	8	141	2.59e-18	76.6
MS.gene62540.t1	MTR_5g008705	34.091	132	77	3	26	152	2	128	1.22e-17	74.3
MS.gene62540.t1	MTR_4g112460	32.168	143	93	3	13	152	6	147	1.36e-17	75.5
MS.gene62540.t1	MTR_7g074240	35.374	147	77	4	21	152	27	170	3.10e-17	74.7
MS.gene62540.t1	MTR_8g069915	28.873	142	97	3	14	152	8	148	1.18e-15	70.1
MS.gene62540.t1	MTR_5g027000	28.289	152	107	2	2	152	6	156	2.35e-15	69.3
MS.gene62540.t1	MTR_5g089320	28.758	153	103	3	2	152	357	505	3.18e-15	72.0
MS.gene62540.t1	MTR_1g030440	33.557	149	81	3	16	153	20	161	4.64e-15	68.6
MS.gene62540.t1	MTR_7g090450	28.082	146	100	2	12	152	67	212	1.93e-14	68.2
MS.gene62540.t1	MTR_0028s0170	30.519	154	99	4	3	152	366	515	2.10e-14	69.7
MS.gene62540.t1	MTR_3g088655	32.895	152	78	5	20	153	10	155	7.15e-14	65.5
MS.gene62540.t1	MTR_0168s0120	32.727	110	71	3	45	152	3	111	9.78e-14	63.9
MS.gene62540.t1	MTR_6g025320	32.727	110	71	3	45	152	3	111	9.78e-14	63.9
MS.gene62540.t1	MTR_1g026190	28.571	154	102	4	3	152	370	519	1.09e-13	67.8
MS.gene62540.t1	MTR_5g009830	28.571	154	102	4	3	152	354	503	1.15e-13	67.4
MS.gene62540.t1	MTR_6g007613	29.496	139	96	2	15	152	26	163	1.28e-13	65.1
MS.gene62540.t1	MTR_5g008695	33.333	105	67	1	50	151	8	112	2.88e-13	62.8
MS.gene62540.t1	MTR_5g079470	40.580	69	39	1	16	84	8	74	8.95e-13	60.5
MS.gene62540.t1	MTR_7g074020	29.496	139	96	2	15	152	27	164	1.05e-12	62.4
MS.gene62540.t1	MTR_2g098890	31.206	141	91	3	17	152	63	202	1.17e-12	63.2
MS.gene62540.t1	MTR_7g068710	30.201	149	101	2	6	152	347	494	2.18e-12	63.9
MS.gene62540.t1	MTR_8g099095	28.571	154	102	4	3	152	354	503	3.07e-12	63.5
MS.gene62540.t1	MTR_5g092810	29.221	154	101	4	3	152	380	529	3.10e-12	63.5
MS.gene62540.t1	MTR_4g132070	30.827	133	86	3	22	152	400	528	6.81e-12	62.4
MS.gene62540.t1	MTR_4g132040	29.167	144	96	3	11	152	393	532	6.82e-12	62.4
MS.gene62540.t1	MTR_3g051770	29.114	158	103	4	3	152	371	527	2.12e-11	60.8
MS.gene62540.t1	MTR_7g115040	31.206	141	95	2	14	153	8	147	2.63e-11	58.5
MS.gene62540.t1	MTR_3g098090	28.105	153	103	4	3	152	320	468	7.08e-11	59.3
MS.gene62540.t1	MTR_5g011850	27.083	144	95	3	14	153	8	145	9.28e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62540.t1	AT1G76650	48.000	150	77	1	3	152	28	176	1.29e-41	137
MS.gene62540.t1	AT1G76650	48.000	150	77	1	3	152	28	176	1.29e-41	137
MS.gene62540.t1	AT1G76650	47.482	139	62	1	14	152	39	166	2.58e-37	125
MS.gene62540.t1	AT1G76640	47.183	142	71	2	14	152	18	158	5.58e-37	124
MS.gene62540.t1	AT3G01830	48.592	142	69	2	13	152	6	145	3.24e-35	120
MS.gene62540.t1	AT5G42380	47.143	140	71	2	15	152	46	184	8.18e-35	120
MS.gene62540.t1	AT3G50770	45.652	138	68	3	21	152	67	203	2.37e-33	117
MS.gene62540.t1	AT1G24620	42.446	139	78	2	15	152	34	171	8.50e-31	109
MS.gene62540.t1	AT5G37770	42.754	138	73	2	20	152	19	155	3.56e-30	107
MS.gene62540.t1	AT5G37770	42.754	138	73	2	20	152	19	155	3.56e-30	107
MS.gene62540.t1	AT1G66400	40.876	137	77	2	20	153	17	152	1.71e-27	100
MS.gene62540.t1	AT1G18210	36.429	140	85	2	13	152	18	153	1.65e-23	90.9
MS.gene62540.t1	AT1G18210	36.429	140	85	2	13	152	18	153	1.65e-23	90.9
MS.gene62540.t1	AT5G17470	37.778	135	78	3	22	152	6	138	1.24e-22	87.8
MS.gene62540.t1	AT5G21274	34.043	141	90	3	14	152	8	147	3.67e-22	86.7
MS.gene62540.t1	AT5G21274	41.791	67	39	0	86	152	7	73	2.84e-11	58.5
MS.gene62540.t1	AT3G56800	33.333	141	91	3	14	152	8	147	6.43e-22	85.9
MS.gene62540.t1	AT3G56800	41.791	67	39	0	86	152	7	73	2.84e-11	58.5
MS.gene62540.t1	AT2G27030	33.333	141	91	3	14	152	8	147	6.43e-22	85.9
MS.gene62540.t1	AT2G27030	41.791	67	39	0	86	152	7	73	2.84e-11	58.5
MS.gene62540.t1	AT2G41110	33.333	141	91	3	14	152	8	147	6.43e-22	85.9
MS.gene62540.t1	AT2G41110	41.791	67	39	0	86	152	7	73	2.84e-11	58.5
MS.gene62540.t1	AT1G73630	33.333	135	86	2	18	152	20	150	7.01e-22	86.3
MS.gene62540.t1	AT3G43810	33.333	141	91	3	14	152	8	147	7.24e-22	85.9
MS.gene62540.t1	AT3G43810	41.791	67	39	0	86	152	7	73	2.81e-11	58.5
MS.gene62540.t1	AT2G43290	35.374	147	80	4	17	152	64	206	1.22e-21	87.0
MS.gene62540.t1	AT5G37780	31.915	141	93	3	14	152	8	147	1.66e-21	85.1
MS.gene62540.t1	AT5G37780	43.284	67	38	0	86	152	7	73	7.42e-12	60.1
MS.gene62540.t1	AT1G66410	31.915	141	93	3	14	152	8	147	1.66e-21	85.1
MS.gene62540.t1	AT1G66410	43.284	67	38	0	86	152	7	73	7.42e-12	60.1
MS.gene62540.t1	AT3G22930	30.769	143	97	2	12	153	29	170	2.46e-21	85.1
MS.gene62540.t1	AT2G27030	33.333	141	91	3	14	152	8	147	2.83e-21	85.1
MS.gene62540.t1	AT2G27030	41.791	67	39	0	86	152	7	73	9.44e-11	57.8
MS.gene62540.t1	AT3G43810	33.333	141	91	3	14	152	8	147	3.18e-21	85.9
MS.gene62540.t1	AT3G43810	41.791	67	39	0	86	152	7	73	9.80e-11	58.2
MS.gene62540.t1	AT4G14640	31.206	141	95	2	14	153	9	148	3.66e-21	84.3
MS.gene62540.t1	AT1G05990	35.714	140	85	3	17	152	4	142	5.43e-21	83.6
MS.gene62540.t1	AT2G15680	34.532	139	85	3	16	152	48	182	9.41e-21	84.0
MS.gene62540.t1	AT4G12860	35.664	143	87	4	13	152	1	141	9.97e-21	83.2
MS.gene62540.t1	AT2G36180	34.532	139	80	3	21	152	3	137	1.09e-20	82.8
MS.gene62540.t1	AT2G41100	32.051	156	88	2	14	152	8	162	2.52e-20	85.5
MS.gene62540.t1	AT2G41100	31.210	157	89	2	14	152	97	252	1.13e-19	83.6
MS.gene62540.t1	AT2G41100	32.051	156	88	2	14	152	8	162	2.52e-20	85.5
MS.gene62540.t1	AT2G41100	31.210	157	89	2	14	152	97	252	1.13e-19	83.6
MS.gene62540.t1	AT2G41100	31.210	157	89	2	14	152	62	217	6.75e-20	84.0
MS.gene62540.t1	AT2G41100	29.921	127	71	2	43	152	2	127	2.34e-11	60.5
MS.gene62540.t1	AT2G41100	31.210	157	89	2	14	152	62	217	6.75e-20	84.0
MS.gene62540.t1	AT2G41100	29.921	127	71	2	43	152	2	127	2.34e-11	60.5
MS.gene62540.t1	AT2G41100	31.210	157	89	2	14	152	62	217	6.75e-20	84.0
MS.gene62540.t1	AT2G41100	29.921	127	71	2	43	152	2	127	2.34e-11	60.5
MS.gene62540.t1	AT3G01830	47.273	110	54	2	13	120	6	113	7.17e-20	80.5
MS.gene62540.t1	AT2G41110	30.719	153	91	4	14	152	8	159	1.05e-19	80.5
MS.gene62540.t1	AT3G59440	33.333	135	87	2	20	152	53	186	1.10e-19	81.6
MS.gene62540.t1	AT4G03290	33.099	142	88	3	17	152	4	144	1.46e-18	77.4
MS.gene62540.t1	AT1G18530	33.099	142	91	3	14	152	3	143	2.21e-18	77.0
MS.gene62540.t1	AT3G07490	35.252	139	86	3	17	152	4	141	3.70e-18	76.6
MS.gene62540.t1	AT3G50360	33.094	139	91	2	15	152	24	161	4.23e-18	76.6
MS.gene62540.t1	AT3G10190	31.579	133	90	1	21	152	73	205	1.29e-17	76.3
MS.gene62540.t1	AT5G37780	28.846	156	93	4	14	152	8	162	2.13e-17	74.7
MS.gene62540.t1	AT1G32250	30.986	142	90	3	14	148	12	152	1.18e-16	72.8
MS.gene62540.t1	AT3G03400	33.858	127	80	2	22	145	9	134	2.00e-16	71.6
MS.gene62540.t1	AT5G37780	26.946	167	93	4	14	152	8	173	9.65e-16	70.9
MS.gene62540.t1	AT4G14640	30.894	123	83	2	32	153	9	130	1.05e-15	69.7
MS.gene62540.t1	AT5G37780	31.707	123	81	3	32	152	13	134	1.72e-15	68.9
MS.gene62540.t1	AT3G25600	30.282	142	95	3	14	152	8	148	2.56e-15	69.3
MS.gene62540.t1	AT1G66410	31.707	123	81	3	32	152	36	157	2.65e-15	69.3
MS.gene62540.t1	AT4G37010	28.477	151	104	3	3	152	18	165	3.67e-15	68.9
MS.gene62540.t1	AT4G37010	28.477	151	104	3	3	152	14	161	3.77e-15	68.9
MS.gene62540.t1	AT2G41090	28.777	139	96	2	15	153	9	144	5.56e-15	68.9
MS.gene62540.t1	AT3G51920	29.078	141	98	2	14	153	8	147	8.79e-15	67.8
MS.gene62540.t1	AT1G66410	31.818	110	72	3	45	152	3	111	5.63e-14	64.7
MS.gene62540.t1	AT1G66410	31.818	110	72	3	45	152	3	111	5.63e-14	64.7
MS.gene62540.t1	AT2G41410	29.371	143	98	2	14	153	66	208	5.88e-14	66.6
MS.gene62540.t1	AT4G20780	32.278	158	81	5	18	152	29	183	7.61e-14	65.9
MS.gene62540.t1	AT3G43810	32.727	110	71	3	45	152	3	111	1.02e-13	63.9
MS.gene62540.t1	AT3G43810	32.727	110	71	3	45	152	3	111	1.02e-13	63.9
MS.gene62540.t1	AT1G76040	31.169	154	98	4	3	152	161	310	2.10e-13	66.6
MS.gene62540.t1	AT1G76040	31.169	154	98	4	3	152	161	310	2.10e-13	66.6
MS.gene62540.t1	AT3G03000	30.657	137	92	2	14	148	16	151	2.26e-13	64.3
MS.gene62540.t1	AT5G12180	31.818	154	97	4	3	152	361	510	2.80e-13	66.6
MS.gene62540.t1	AT5G19360	31.169	154	98	4	3	152	356	505	3.95e-13	66.2
MS.gene62540.t1	AT1G76040	31.169	154	98	4	3	152	399	548	4.12e-13	65.9
MS.gene62540.t1	AT1G74740	33.333	135	81	4	22	152	368	497	4.51e-13	65.9
MS.gene62540.t1	AT5G44460	33.333	150	82	6	18	152	28	174	1.08e-12	62.8
MS.gene62540.t1	AT3G03410	31.579	133	82	2	21	153	5	128	1.53e-12	61.2
MS.gene62540.t1	AT5G17480	38.889	72	41	2	9	80	1	69	1.83e-12	59.7
MS.gene62540.t1	AT4G23650	29.221	154	101	4	3	152	366	515	2.05e-12	63.9
MS.gene62540.t1	AT1G12310	28.472	144	93	3	14	153	9	146	6.69e-12	60.1
MS.gene62540.t1	AT3G03430	35.526	76	46	2	9	84	1	73	8.53e-12	58.2
MS.gene62540.t1	AT1G50700	29.221	154	101	4	3	152	361	510	9.55e-12	62.0
MS.gene62540.t1	AT4G04695	28.571	154	102	4	3	152	319	468	1.25e-11	61.6
MS.gene62540.t1	AT2G31500	31.579	152	98	4	6	152	357	507	1.55e-11	61.6
MS.gene62540.t1	AT3G51850	29.008	131	92	1	22	152	363	492	1.77e-11	61.2
MS.gene62540.t1	AT5G19450	27.632	152	108	1	3	152	345	496	7.89e-11	59.3
MS.gene62540.t1	AT5G19450	27.632	152	108	1	3	152	345	496	7.89e-11	59.3

Find 30 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGATGGTGATGGATTGTT+GGG	0.312082	7.3:+69625647	MS.gene62540:CDS
ATCTCTAAGTTTGATCTTGA+TGG	0.328179	7.3:+69625848	MS.gene62540:CDS
CTCAGATGGTGATGGATTGT+TGG	0.354318	7.3:+69625646	MS.gene62540:CDS
GGTTGAACACACGTTCATAT+TGG	0.358450	7.3:-69625505	None:intergenic
GTATGTTATTTGTGATGTTA+TGG	0.366465	7.3:+69625472	MS.gene62540:CDS
AAAAGAATGCTTAGTAGGTT+AGG	0.383946	7.3:+69625791	MS.gene62540:CDS
GTCTTAAAAGAATGCTTAGT+AGG	0.416556	7.3:+69625786	MS.gene62540:CDS
AGAGGCGGCCGTGGCAATCT+TGG	0.417019	7.3:+69625622	MS.gene62540:CDS
GTTTGTGGAGGGTGCAAAAG+AGG	0.441772	7.3:+69625688	MS.gene62540:CDS
AAGTTTGATCTTGATGGGGA+TGG	0.469441	7.3:+69625854	MS.gene62540:CDS
TCTCTAAGTTTGATCTTGAT+GGG	0.474554	7.3:+69625849	MS.gene62540:CDS
GATCGGTGATGAAAAGTTAT+CGG	0.478907	7.3:+69625589	MS.gene62540:CDS
AGAGGATGTAGAGGCGGCCG+TGG	0.516831	7.3:+69625613	MS.gene62540:CDS
TTTAAGTTGTATGAGATGGA+TGG	0.526008	7.3:+69625740	MS.gene62540:CDS
AGTTTGATCTTGATGGGGAT+GGG	0.530544	7.3:+69625855	MS.gene62540:CDS
GAAAGTGAATGACTTAAAAG+AGG	0.531408	7.3:+69625715	MS.gene62540:CDS
ACACATTGTCGCAGCTCAGC+CGG	0.534198	7.3:-69625560	None:intergenic
TGATGAAAAGTTATCGGCAG+AGG	0.566777	7.3:+69625595	MS.gene62540:CDS
CTCTAAGTTTGATCTTGATG+GGG	0.585858	7.3:+69625850	MS.gene62540:CDS
GTGGCAATCTTGGACTCAGA+TGG	0.588921	7.3:+69625632	MS.gene62540:CDS
CAGATGGTGATGGATTGTTG+GGG	0.592987	7.3:+69625648	MS.gene62540:CDS
TTGTATGAGATGGATGGAAG+TGG	0.607357	7.3:+69625746	MS.gene62540:CDS
ATCTTGGACTCAGATGGTGA+TGG	0.610181	7.3:+69625638	MS.gene62540:CDS
TGGAGATGGTAAGATATCGC+CGG	0.613279	7.3:+69625541	MS.gene62540:CDS
CGACAATGTGTTGAGGCGAT+CGG	0.636267	7.3:+69625572	MS.gene62540:CDS
GTTATCGGCAGAGGATGTAG+AGG	0.638879	7.3:+69625604	MS.gene62540:CDS
ATCTGAGTCCAAGATTGCCA+CGG	0.643059	7.3:-69625630	None:intergenic
TATTTGTGATGTTATGGACA+AGG	0.644981	7.3:+69625478	MS.gene62540:CDS
ATCGGCAGAGGATGTAGAGG+CGG	0.662701	7.3:+69625607	MS.gene62540:CDS
TGAGCTGCGACAATGTGTTG+AGG	0.706960	7.3:+69625565	MS.gene62540:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATCAGTTTTGATGAATTTA+AGG	+	chr7.3:69625880-69625899	MS.gene62540:CDS	20.0%
!!	GTATGTTATTTGTGATGTTA+TGG	+	chr7.3:69625472-69625491	MS.gene62540:CDS	25.0%
	AAAAGAATGCTTAGTAGGTT+AGG	+	chr7.3:69625791-69625810	MS.gene62540:CDS	30.0%
	ATCTCTAAGTTTGATCTTGA+TGG	+	chr7.3:69625848-69625867	MS.gene62540:CDS	30.0%
	GAAAGTGAATGACTTAAAAG+AGG	+	chr7.3:69625715-69625734	MS.gene62540:CDS	30.0%
	GTCTTAAAAGAATGCTTAGT+AGG	+	chr7.3:69625786-69625805	MS.gene62540:CDS	30.0%
	TATTTGTGATGTTATGGACA+AGG	+	chr7.3:69625478-69625497	MS.gene62540:CDS	30.0%
	TCTCTAAGTTTGATCTTGAT+GGG	+	chr7.3:69625849-69625868	MS.gene62540:CDS	30.0%
!	CATCTCCATTTTCATCAAAA+CGG	-	chr7.3:69625529-69625548	None:intergenic	30.0%
!	TGATGATTTTGTGAAGTTTG+TGG	+	chr7.3:69625673-69625692	MS.gene62540:CDS	30.0%
!!	TTCAACCGTTTTGATGAAAA+TGG	+	chr7.3:69625521-69625540	MS.gene62540:CDS	30.0%
!!	TTTAAGTTGTATGAGATGGA+TGG	+	chr7.3:69625740-69625759	MS.gene62540:CDS	30.0%
!!!	AGCATTCTTTTAAGACTCTT+AGG	-	chr7.3:69625782-69625801	None:intergenic	30.0%
	CTCTAAGTTTGATCTTGATG+GGG	+	chr7.3:69625850-69625869	MS.gene62540:CDS	35.0%
	GATCGGTGATGAAAAGTTAT+CGG	+	chr7.3:69625589-69625608	MS.gene62540:CDS	35.0%
!	GATTTTGTGAAGTTTGTGGA+GGG	+	chr7.3:69625677-69625696	MS.gene62540:CDS	35.0%
!	TGATTTTGTGAAGTTTGTGG+AGG	+	chr7.3:69625676-69625695	MS.gene62540:CDS	35.0%
!!	CGTTTTGATGAAAATGGAGA+TGG	+	chr7.3:69625527-69625546	MS.gene62540:CDS	35.0%
!!!	GGCTTTTAAGTTGTATGAGA+TGG	+	chr7.3:69625736-69625755	MS.gene62540:CDS	35.0%
	AAGTTTGATCTTGATGGGGA+TGG	+	chr7.3:69625854-69625873	MS.gene62540:CDS	40.0%
	AGTTTGATCTTGATGGGGAT+GGG	+	chr7.3:69625855-69625874	MS.gene62540:CDS	40.0%
	GGTTGAACACACGTTCATAT+TGG	-	chr7.3:69625508-69625527	None:intergenic	40.0%
	TGATGAAAAGTTATCGGCAG+AGG	+	chr7.3:69625595-69625614	MS.gene62540:CDS	40.0%
	TTGTATGAGATGGATGGAAG+TGG	+	chr7.3:69625746-69625765	MS.gene62540:CDS	40.0%
!!	TCAGATGGTGATGGATTGTT+GGG	+	chr7.3:69625647-69625666	MS.gene62540:CDS	40.0%
	ATCTGAGTCCAAGATTGCCA+CGG	-	chr7.3:69625633-69625652	None:intergenic	45.0%
	ATCTTGGACTCAGATGGTGA+TGG	+	chr7.3:69625638-69625657	MS.gene62540:CDS	45.0%
	TGGAGATGGTAAGATATCGC+CGG	+	chr7.3:69625541-69625560	MS.gene62540:CDS	45.0%
!!	CAGATGGTGATGGATTGTTG+GGG	+	chr7.3:69625648-69625667	MS.gene62540:CDS	45.0%
!!	CTCAGATGGTGATGGATTGT+TGG	+	chr7.3:69625646-69625665	MS.gene62540:CDS	45.0%
	CGACAATGTGTTGAGGCGAT+CGG	+	chr7.3:69625572-69625591	MS.gene62540:CDS	50.0%
	GTGGCAATCTTGGACTCAGA+TGG	+	chr7.3:69625632-69625651	MS.gene62540:CDS	50.0%
	GTTATCGGCAGAGGATGTAG+AGG	+	chr7.3:69625604-69625623	MS.gene62540:CDS	50.0%
	TGAGCTGCGACAATGTGTTG+AGG	+	chr7.3:69625565-69625584	MS.gene62540:CDS	50.0%
!	GTTTGTGGAGGGTGCAAAAG+AGG	+	chr7.3:69625688-69625707	MS.gene62540:CDS	50.0%
	ACACATTGTCGCAGCTCAGC+CGG	-	chr7.3:69625563-69625582	None:intergenic	55.0%
	ATCGGCAGAGGATGTAGAGG+CGG	+	chr7.3:69625607-69625626	MS.gene62540:CDS	55.0%
	AGAGGATGTAGAGGCGGCCG+TGG	+	chr7.3:69625613-69625632	MS.gene62540:CDS	65.0%
	AGAGGCGGCCGTGGCAATCT+TGG	+	chr7.3:69625622-69625641	MS.gene62540:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	69625455	69625916	69625455	ID=MS.gene62540
chr7.3	mRNA	69625455	69625916	69625455	ID=MS.gene62540.t1;Parent=MS.gene62540
chr7.3	exon	69625455	69625916	69625455	ID=MS.gene62540.t1.exon1;Parent=MS.gene62540.t1
chr7.3	CDS	69625455	69625916	69625455	ID=cds.MS.gene62540.t1;Parent=MS.gene62540.t1

Gene Sequence

>MS.gene62540

ATCATAAAAATAAAGTTGTATGTTATTTGTGATGTTATGGACAAGGTAAGCCAATATGAACGTGTGTTCAACCGTTTTGATGAAAATGGAGATGGTAAGATATCGCCGGCTGAGCTGCGACAATGTGTTGAGGCGATCGGTGATGAAAAGTTATCGGCAGAGGATGTAGAGGCGGCCGTGGCAATCTTGGACTCAGATGGTGATGGATTGTTGGGGTTTGATGATTTTGTGAAGTTTGTGGAGGGTGCAAAAGAGGAAGAGAAAGTGAATGACTTAAAAGAGGCTTTTAAGTTGTATGAGATGGATGGAAGTGGTTGCATAACACCTAAGAGTCTTAAAAGAATGCTTAGTAGGTTAGGTGATTCTAGAAGTATAGATGAGTGTCAACTTATGATCTCTAAGTTTGATCTTGATGGGGATGGGAAAATCAGTTTTGATGAATTTAAGGTCATGATGTTGTAA

Protein sequence

>MS.gene62540.t1

IIKIKLYVICDVMDKVSQYERVFNRFDENGDGKISPAELRQCVEAIGDEKLSAEDVEAAVAILDSDGDGLLGFDDFVKFVEGAKEEEKVNDLKEAFKLYEMDGSGCITPKSLKRMLSRLGDSRSIDECQLMISKFDLDGDGKISFDEFKVMML