AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64658.t1 MTR_5g079740 99.552 223 1 0 1 223 1 223 6.89e-170 466
MS.gene64658.t1 MTR_2g098010 99.103 223 2 0 1 223 1 223 4.60e-169 464
MS.gene64658.t1 MTR_5g079740 99.398 166 1 0 58 223 1 166 1.88e-122 344
MS.gene64658.t1 MTR_7g088490 32.558 129 87 0 9 137 2 130 5.83e-18 79.3
MS.gene64658.t1 MTR_4g087680 32.283 127 86 0 11 137 4 130 9.94e-17 75.9
MS.gene64658.t1 MTR_3g109210 27.273 209 126 4 15 217 13 201 4.03e-16 74.7
MS.gene64658.t1 MTR_4g023520 94.118 34 2 0 58 91 1 34 5.91e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64658.t1 AT3G60820 86.996 223 29 0 1 223 1 223 7.59e-150 416
MS.gene64658.t1 AT3G60820 86.996 223 29 0 1 223 1 223 7.59e-150 416
MS.gene64658.t1 AT3G60820 86.099 223 31 0 1 223 1 223 7.52e-148 410
MS.gene64658.t1 AT1G21720 32.558 129 87 0 9 137 2 130 2.30e-18 80.5
MS.gene64658.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.89e-17 77.8
MS.gene64658.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.89e-17 77.8
MS.gene64658.t1 AT4G31300 26.794 209 127 4 15 217 12 200 2.09e-13 67.4
MS.gene64658.t1 AT4G31300 26.794 209 127 4 15 217 12 200 2.09e-13 67.4
MS.gene64658.t1 AT4G31300 24.878 205 137 3 15 217 12 201 2.38e-12 64.7

Find 50 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTATTCTTTCAATGTTT+TGG 0.153956 5.2:-67779883 MS.gene64658:CDS
GATGAATACCACTCGCATTT+AGG 0.297426 5.2:+67778824 None:intergenic
TGACCAAACAACACGCTAAT+TGG 0.299095 5.2:-67782421 MS.gene64658:CDS
AGATCTTGTGTTGCAATTGC+TGG 0.324649 5.2:-67782283 MS.gene64658:CDS
AGCGTACAAGTGTGAGATTT+TGG 0.327605 5.2:+67782189 None:intergenic
AAATGTGTTATGGCTTCTTC+TGG 0.334407 5.2:-67782063 MS.gene64658:CDS
GGATATAGCTCTCAAGGTTC+TGG 0.342345 5.2:-67779710 MS.gene64658:CDS
AAAACATTGAAAGAATAATA+AGG 0.377276 5.2:+67779885 None:intergenic
TGAAACAGAAGCAGTTGATT+TGG 0.385508 5.2:-67779204 MS.gene64658:CDS
GAAAGTAACTGAGCCATTGC+AGG 0.408497 5.2:+67779936 None:intergenic
CGCGGCGATAACACAGTAAT+CGG 0.412401 5.2:+67782258 None:intergenic
TACCCTCCATTGTTGTCATA+TGG 0.417558 5.2:+67782395 None:intergenic
GCTATATCCCACCCTTTCAT+AGG 0.421527 5.2:+67779724 None:intergenic
ACTTCACCTGTGCAGGTAAG+AGG 0.429182 5.2:+67779633 None:intergenic
TTTAGTTGGTTATCAAGAAA+AGG 0.431130 5.2:+67779671 None:intergenic
AGACCAATTAGCGTGTTGTT+TGG 0.453572 5.2:+67782418 None:intergenic
GTGGACTGGGAGACTTTAGT+TGG 0.471755 5.2:+67779657 None:intergenic
TTTACGAACTTCACCTGTGC+AGG 0.482932 5.2:+67779626 None:intergenic
TGGTATTCATCGTGAATTCA+TGG 0.486554 5.2:-67778812 MS.gene64658:CDS
AAAAGCATCAGTCTTTCCTT+AGG 0.494518 5.2:+67778788 None:intergenic
ACGAGTGAGGATGTTGTAAC+CGG 0.502203 5.2:+67782219 None:intergenic
CAGGGCTGAGAAATGTGTTA+TGG 0.517173 5.2:-67782073 MS.gene64658:CDS
GCAGGTAAGAGGAGTGGACT+GGG 0.519527 5.2:+67779644 None:intergenic
GCAAATGAGCTGCCCTGCAA+TGG 0.526195 5.2:-67779949 MS.gene64658:CDS
TGCAGGTAAGAGGAGTGGAC+TGG 0.534103 5.2:+67779643 None:intergenic
ATCGTGAATTCATGGACCTA+AGG 0.538648 5.2:-67778804 MS.gene64658:CDS
ATGCTGTTGGTTCCTATGAA+AGG 0.538907 5.2:-67779736 MS.gene64658:CDS
TCCACTCCTCTTACCTGCAC+AGG 0.541323 5.2:-67779639 MS.gene64658:intron
GTCTTCACATATGATGCTGT+TGG 0.551042 5.2:-67779749 MS.gene64658:CDS
TTGGGCGGCCTCGACAGTGA+AGG 0.558929 5.2:-67779864 MS.gene64658:intron
TGATGTCAAGGCTTTGCAGA+AGG 0.566876 5.2:-67782031 MS.gene64658:CDS
AGGGTGGGATATAGCTCTCA+AGG 0.576238 5.2:-67779716 MS.gene64658:CDS
AAAGTAACTGAGCCATTGCA+GGG 0.583094 5.2:+67779937 None:intergenic
CTCCATATGACAACAATGGA+GGG 0.588306 5.2:-67782397 MS.gene64658:intron
ACCTGTGCAGGTAAGAGGAG+TGG 0.590257 5.2:+67779638 None:intergenic
ACTGCTTCTGTTTCAGACAG+TGG 0.593751 5.2:+67779212 None:intergenic
TGCTGTTGGTTCCTATGAAA+GGG 0.597669 5.2:-67779735 MS.gene64658:CDS
AAGAAAAGGCATAATGAGTG+TGG 0.597780 5.2:+67779685 None:intergenic
ATTAGCGTGTTGTTTGGTCA+TGG 0.614741 5.2:+67782424 None:intergenic
GTGTTATCGCCGCGGATACG+AGG 0.627619 5.2:-67782249 MS.gene64658:CDS
TCTCCATATGACAACAATGG+AGG 0.635572 5.2:-67782398 MS.gene64658:CDS
TGGTCTCCATATGACAACAA+TGG 0.646174 5.2:-67782401 MS.gene64658:CDS
TTCAACCTGAATTAGGAAAG+GGG 0.647601 5.2:-67779775 MS.gene64658:intron
TGTTGGTTCCTATGAAAGGG+TGG 0.648410 5.2:-67779732 MS.gene64658:CDS
ATAGTGCACCTTCACTGTCG+AGG 0.654158 5.2:+67779856 None:intergenic
ATTGTCATCCTAAATGCGAG+TGG 0.663862 5.2:-67778832 MS.gene64658:CDS
GGTAGACATCCTCGTATCCG+CGG 0.665904 5.2:+67782240 None:intergenic
GTTGGTTCCTATGAAAGGGT+GGG 0.677680 5.2:-67779731 MS.gene64658:CDS
GCGGATACGAGGATGTCTAC+CGG 0.703907 5.2:-67782238 MS.gene64658:CDS
CGATTACTGTGTTATCGCCG+CGG 0.718453 5.2:-67782257 MS.gene64658:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATTTATAAATTTCTTTA+TGG - chr5.2:67780801-67780820 MS.gene64658:intron 10.0%
!!! AATAAAATAATAATCACTTT+TGG + chr5.2:67781895-67781914 None:intergenic 10.0%
!!! CTATTTTTTCAATAATTTTT+TGG - chr5.2:67778881-67778900 MS.gene64658:intron 10.0%
!! AAAACATTGAAAGAATAATA+AGG + chr5.2:67781335-67781354 None:intergenic 15.0%
!! AAAATGACATTTATGTAAAA+GGG + chr5.2:67782304-67782323 None:intergenic 15.0%
!! AATCTAAATAGTATAACTTA+GGG + chr5.2:67779630-67779649 None:intergenic 15.0%
!! ATTAAAATATGATTTATTGC+AGG - chr5.2:67781971-67781990 MS.gene64658:intron 15.0%
!! TAAAATGACATTTATGTAAA+AGG + chr5.2:67782305-67782324 None:intergenic 15.0%
!! TTAAAAAAATTAGAATCTGA+AGG + chr5.2:67781855-67781874 None:intergenic 15.0%
!!! TAAATCATATTTTAATGTAG+AGG + chr5.2:67781967-67781986 None:intergenic 15.0%
!!! TAATTTAGAGATACTTTTTT+TGG - chr5.2:67781689-67781708 MS.gene64658:intron 15.0%
!!! TATGAAAATAAGTTGAAAAA+AGG - chr5.2:67779409-67779428 MS.gene64658:intron 15.0%
!!! TATTATTCTTTCAATGTTTT+GGG - chr5.2:67781335-67781354 MS.gene64658:intron 15.0%
!!! TTATTATTCTTTCAATGTTT+TGG - chr5.2:67781334-67781353 MS.gene64658:intron 15.0%
!! AATCTAAATAGTACTACTTA+GGG + chr5.2:67779533-67779552 None:intergenic 20.0%
!! AATGTATAGTGATTTATGTT+TGG - chr5.2:67779755-67779774 MS.gene64658:CDS 20.0%
!! CAATCTAAATAGTATAACTT+AGG + chr5.2:67779631-67779650 None:intergenic 20.0%
!! GATATACAATTATATGTTGA+GGG + chr5.2:67780659-67780678 None:intergenic 20.0%
!! GTAACTTCTATATAGAAAAA+TGG + chr5.2:67780637-67780656 None:intergenic 20.0%
!! TATAGTCAAACTTACTTATA+GGG + chr5.2:67780476-67780495 None:intergenic 20.0%
!! TGAAATATTTGATGCTTTAA+GGG - chr5.2:67781624-67781643 MS.gene64658:intron 20.0%
!! TTGAAATATTTGATGCTTTA+AGG - chr5.2:67781623-67781642 MS.gene64658:intron 20.0%
!!! AAATTTTGTGATACTGTTTT+GGG + chr5.2:67779471-67779490 None:intergenic 20.0%
!!! ACTTTTTTTGGAGATTTTTA+AGG - chr5.2:67781701-67781720 MS.gene64658:intron 20.0%
!!! CATAAATGTCATTTTACATT+AGG - chr5.2:67782309-67782328 MS.gene64658:intron 20.0%
!!! CTATAAGTTGTTTTTGTATA+AGG - chr5.2:67779372-67779391 MS.gene64658:intron 20.0%
!!! CTTTAAGTTAGAATCAATTT+TGG - chr5.2:67779858-67779877 MS.gene64658:intron 20.0%
!!! TAAATTTTGTGATACTGTTT+TGG + chr5.2:67779472-67779491 None:intergenic 20.0%
!!! TAAGGTTTTCTTTACAATTT+TGG + chr5.2:67780681-67780700 None:intergenic 20.0%
!!! TGTTTTTGTATAAGGTATAT+TGG - chr5.2:67779380-67779399 MS.gene64658:intron 20.0%
! AAATAAAGTCATACAGACTA+AGG + chr5.2:67780699-67780718 None:intergenic 25.0%
! AACTAATTCAGAAACATGTT+AGG + chr5.2:67780752-67780771 None:intergenic 25.0%
! AATAAAATAGTACTCCTCTA+GGG - chr5.2:67780304-67780323 MS.gene64658:intron 25.0%
! ACATCAGTAATGTTACAAAT+AGG + chr5.2:67780263-67780282 None:intergenic 25.0%
! CAATCTAAATAGTACTACTT+AGG + chr5.2:67779534-67779553 None:intergenic 25.0%
! CATCAGTAATGTTACAAATA+GGG + chr5.2:67780262-67780281 None:intergenic 25.0%
! CTCGTTTAATAAAGATTGAA+CGG + chr5.2:67782149-67782168 None:intergenic 25.0%
! CTCTAACTACATAATTAATC+AGG + chr5.2:67780902-67780921 None:intergenic 25.0%
! CTGGTATAGTTAAACTTATA+GGG + chr5.2:67780539-67780558 None:intergenic 25.0%
! GGAACATTACAATAATAGAA+AGG + chr5.2:67781392-67781411 None:intergenic 25.0%
! GGATATACAATTATATGTTG+AGG + chr5.2:67780660-67780679 None:intergenic 25.0%
! GTATAGTCAAACTTACTTAT+AGG + chr5.2:67780477-67780496 None:intergenic 25.0%
! TATTCTTAACTATACAAAGC+AGG - chr5.2:67780383-67780402 MS.gene64658:intron 25.0%
! TCAAGGTAAAATTAGAACAT+GGG + chr5.2:67780992-67781011 None:intergenic 25.0%
! TCTGGTATAGTTAAACTTAT+AGG + chr5.2:67780540-67780559 None:intergenic 25.0%
! TGATTATGTGAAACTATTTC+TGG - chr5.2:67779718-67779737 MS.gene64658:CDS 25.0%
! TTAACCAAAAAAGATATCCA+AGG + chr5.2:67780184-67780203 None:intergenic 25.0%
! TTTAGTTGGTTATCAAGAAA+AGG + chr5.2:67781549-67781568 None:intergenic 25.0%
!! ATATCTGAAACTCAAAACAA+AGG + chr5.2:67780339-67780358 None:intergenic 25.0%
!! GACAAATGTTTAACTTACTT+TGG - chr5.2:67782331-67782350 MS.gene64658:intron 25.0%
!! TAGATGTGTAGAATGAATTT+TGG + chr5.2:67779919-67779938 None:intergenic 25.0%
!! TGACGCAAATTAATTTTCAA+TGG - chr5.2:67779693-67779712 MS.gene64658:CDS 25.0%
!! TTTAGATTGCACATATTTTC+AGG - chr5.2:67779642-67779661 MS.gene64658:CDS 25.0%
!!! AGAATGAATTTTGGAATTTG+CGG + chr5.2:67779910-67779929 None:intergenic 25.0%
!!! TATGTTGTTGTTGTTGTTTA+AGG - chr5.2:67779098-67779117 MS.gene64658:intron 25.0%
!!! TTTTTGTTTGTTTTGTGACA+GGG - chr5.2:67779126-67779145 MS.gene64658:intron 25.0%
!!! TTTTTTGTTTGTTTTGTGAC+AGG - chr5.2:67779125-67779144 MS.gene64658:intron 25.0%
AAAAAAAACAGTGTCCAACA+TGG + chr5.2:67779076-67779095 None:intergenic 30.0%
AAATTATGCTATACCTTCTG+CGG - chr5.2:67780964-67780983 MS.gene64658:intron 30.0%
AATGAGAAGTTGTTCTTAGT+AGG + chr5.2:67781808-67781827 None:intergenic 30.0%
ACATTAGTGTTGTCAAATAG+CGG - chr5.2:67781040-67781059 MS.gene64658:intron 30.0%
AGAAAAAAGATGTAGCAAAC+AGG - chr5.2:67781918-67781937 MS.gene64658:intron 30.0%
CAAACCAATGTTGTCAAATA+TGG - chr5.2:67779970-67779989 MS.gene64658:CDS 30.0%
CCAAAATAATCGAATAACTC+TGG + chr5.2:67780558-67780577 None:intergenic 30.0%
CGTTCAATCTTTATTAAACG+AGG - chr5.2:67782147-67782166 MS.gene64658:intron 30.0%
CTTATAGGGCTATCATATTA+AGG + chr5.2:67780525-67780544 None:intergenic 30.0%
CTTATAGGGCTATTACATTA+AGG + chr5.2:67780462-67780481 None:intergenic 30.0%
GAATAAAATAGTACTCCTCT+AGG - chr5.2:67780303-67780322 MS.gene64658:intron 30.0%
GTCAAGGTAAAATTAGAACA+TGG + chr5.2:67780993-67781012 None:intergenic 30.0%
GTTAGTTAGAGATTTAGTGA+GGG - chr5.2:67780924-67780943 MS.gene64658:intron 30.0%
TCAAATAAGCGATTTCAAAC+TGG - chr5.2:67779507-67779526 MS.gene64658:intron 30.0%
TCCAAATTGTTGTCAAATAG+TGG - chr5.2:67781114-67781133 MS.gene64658:intron 30.0%
TGTTAGTTAAGCACAGTTTA+GGG + chr5.2:67781944-67781963 None:intergenic 30.0%
TGTTAGTTAGAGATTTAGTG+AGG - chr5.2:67780923-67780942 MS.gene64658:intron 30.0%
TTAATAAAGATTGAACGGTC+TGG + chr5.2:67782144-67782163 None:intergenic 30.0%
! CATATTTGACAACATTGGTT+TGG + chr5.2:67779972-67779991 None:intergenic 30.0%
! CCAGAGTTATTCGATTATTT+TGG - chr5.2:67780555-67780574 MS.gene64658:intron 30.0%
! GGCTATCATATTAAGGATTT+TGG + chr5.2:67780518-67780537 None:intergenic 30.0%
! TGAGTTGAATAGTACATTCA+AGG - chr5.2:67779795-67779814 MS.gene64658:intron 30.0%
! TTATTAAACGAGGCACATTT+TGG - chr5.2:67782157-67782176 MS.gene64658:intron 30.0%
! TTGCATTGGTGGTTTATTTA+TGG - chr5.2:67780416-67780435 MS.gene64658:intron 30.0%
!! TATTCTTTCAATGTTTTGGG+CGG - chr5.2:67781338-67781357 MS.gene64658:intron 30.0%
!! TATTTTTGCTCTTCCAAAAG+CGG - chr5.2:67779944-67779963 MS.gene64658:CDS 30.0%
!! TTTGGATGTATGTTTTTGCA+GGG - chr5.2:67782349-67782368 MS.gene64658:intron 30.0%
!!! CATTCCTTGGATATCTTTTT+TGG - chr5.2:67780177-67780196 MS.gene64658:intron 30.0%
AAAGGAACATTTAACCCTAG+AGG + chr5.2:67780321-67780340 None:intergenic 35.0%
AAATGTGTTATGGCTTCTTC+TGG - chr5.2:67779154-67779173 MS.gene64658:intron 35.0%
AATGTTGTCAAATATGGCCA+TGG - chr5.2:67779976-67779995 MS.gene64658:intron 35.0%
ACTTCAACCTGAATTAGGAA+AGG - chr5.2:67781440-67781459 MS.gene64658:intron 35.0%
ATCATCAAACCAATCAACCA+CGG + chr5.2:67780831-67780850 None:intergenic 35.0%
ATGGCCATATTTGACAACAT+TGG + chr5.2:67779977-67779996 None:intergenic 35.0%
CAAATATCTGTTCGATAGTG+CGG + chr5.2:67781072-67781091 None:intergenic 35.0%
CATAGAAACACTGCGAAAAA+GGG + chr5.2:67780585-67780604 None:intergenic 35.0%
CGAGTCAGCAAATACAAAAA+TGG + chr5.2:67781413-67781432 None:intergenic 35.0%
CTTCAACCTGAATTAGGAAA+GGG - chr5.2:67781441-67781460 MS.gene64658:intron 35.0%
CTTCTACTTCAACCTGAATT+AGG - chr5.2:67781435-67781454 MS.gene64658:intron 35.0%
GCCACTATTTGACAACAATT+TGG + chr5.2:67781118-67781137 None:intergenic 35.0%
GGTCATCAACATTGTATGTT+TGG + chr5.2:67780137-67780156 None:intergenic 35.0%
GGTTTATTTATGGACTCTCA+TGG - chr5.2:67780426-67780445 MS.gene64658:intron 35.0%
GTACTTCGACTTTGCAAAAT+AGG + chr5.2:67782195-67782214 None:intergenic 35.0%
GTCACAAAACATAAGCAAGT+AGG - chr5.2:67779576-67779595 MS.gene64658:intron 35.0%
GTGTGAATGCAGAGTTATTT+CGG - chr5.2:67782262-67782281 MS.gene64658:CDS 35.0%
GTGTTAGTTAAGCACAGTTT+AGG + chr5.2:67781945-67781964 None:intergenic 35.0%
GTGTTGAATATTTGCATTGG+TGG - chr5.2:67780405-67780424 MS.gene64658:intron 35.0%
TAGAAAAATGGCGAAGAGTT+TGG + chr5.2:67780625-67780644 None:intergenic 35.0%
TCAAATAAGCGATTCCAAAC+AGG - chr5.2:67779604-67779623 MS.gene64658:intron 35.0%
TCATAGAAACACTGCGAAAA+AGG + chr5.2:67780586-67780605 None:intergenic 35.0%
TGAAACAGAAGCAGTTGATT+TGG - chr5.2:67782013-67782032 MS.gene64658:CDS 35.0%
TGACAAAAAATTGAAGCCTC+TGG - chr5.2:67781009-67781028 MS.gene64658:intron 35.0%
TGGTATTCATCGTGAATTCA+TGG - chr5.2:67782405-67782424 MS.gene64658:CDS 35.0%
TTCAACCTGAATTAGGAAAG+GGG - chr5.2:67781442-67781461 MS.gene64658:intron 35.0%
TTTGGTTAAGTTCGTGAGTT+AGG - chr5.2:67780195-67780214 MS.gene64658:intron 35.0%
! AAGAAAAGGCATAATGAGTG+TGG + chr5.2:67781535-67781554 None:intergenic 35.0%
! ACTAATGTCATTTCAACCAG+AGG + chr5.2:67781028-67781047 None:intergenic 35.0%
! ATGACCAATTTTGTGAAGGA+TGG - chr5.2:67780032-67780051 MS.gene64658:intron 35.0%
! CAGCCAGTATTTCAGATTTT+TGG - chr5.2:67780077-67780096 MS.gene64658:intron 35.0%
! GAGCCAAAAATCTGAAATAC+TGG + chr5.2:67780083-67780102 None:intergenic 35.0%
!! CACATATTTTCAGGATGACT+TGG - chr5.2:67779651-67779670 MS.gene64658:CDS 35.0%
!! CAGGTGTTGAATATTTGCAT+TGG - chr5.2:67780402-67780421 MS.gene64658:intron 35.0%
!! CTTTGGATGTATGTTTTTGC+AGG - chr5.2:67782348-67782367 MS.gene64658:intron 35.0%
!! TTGGATGTATGTTTTTGCAG+GGG - chr5.2:67782350-67782369 MS.gene64658:intron 35.0%
!!! GAGGCTTCAATTTTTTGTCA+AGG + chr5.2:67781009-67781028 None:intergenic 35.0%
AAAGTAACTGAGCCATTGCA+GGG + chr5.2:67781283-67781302 None:intergenic 40.0%
AAGGAGTCATCAACATTCCT+TGG - chr5.2:67780164-67780183 MS.gene64658:intron 40.0%
AATATGGCCATGGAGGATAT+AGG - chr5.2:67779986-67780005 MS.gene64658:intron 40.0%
AGATCTTGTGTTGCAATTGC+TGG - chr5.2:67778934-67778953 MS.gene64658:intron 40.0%
AGTATAACTTAGGGCCTGTT+TGG + chr5.2:67779621-67779640 None:intergenic 40.0%
ATATGACAACAATGGAGGGT+AGG - chr5.2:67778824-67778843 MS.gene64658:CDS 40.0%
ATCATGCATTCACGCTATTG+CGG - chr5.2:67782116-67782135 MS.gene64658:intron 40.0%
ATCGTGAATTCATGGACCTA+AGG - chr5.2:67782413-67782432 MS.gene64658:CDS 40.0%
ATTGTCATCCTAAATGCGAG+TGG - chr5.2:67782385-67782404 MS.gene64658:intron 40.0%
ATTTCAGCCGCCTTATAAGA+GGG + chr5.2:67782233-67782252 None:intergenic 40.0%
CTCCATATGACAACAATGGA+GGG - chr5.2:67778820-67778839 MS.gene64658:CDS 40.0%
CTTATAAGAGGGACTCAAGA+GGG + chr5.2:67782222-67782241 None:intergenic 40.0%
GATGAATACCACTCGCATTT+AGG + chr5.2:67782396-67782415 None:intergenic 40.0%
GCTATAACACTGTAGCATAG+CGG - chr5.2:67781147-67781166 MS.gene64658:intron 40.0%
GTCTTCACATATGATGCTGT+TGG - chr5.2:67781468-67781487 MS.gene64658:intron 40.0%
GTTAAGTTCGTGAGTTAGGA+AGG - chr5.2:67780199-67780218 MS.gene64658:intron 40.0%
GTTAGGAAGGAAACTTTGCT+AGG - chr5.2:67780212-67780231 MS.gene64658:intron 40.0%
TACCCTCCATTGTTGTCATA+TGG + chr5.2:67778825-67778844 None:intergenic 40.0%
TATGACAACAATGGAGGGTA+GGG - chr5.2:67778825-67778844 MS.gene64658:CDS 40.0%
TCTCCATATGACAACAATGG+AGG - chr5.2:67778819-67778838 MS.gene64658:CDS 40.0%
TGACCAAACAACACGCTAAT+TGG - chr5.2:67778796-67778815 MS.gene64658:CDS 40.0%
TGGTCTCCATATGACAACAA+TGG - chr5.2:67778816-67778835 MS.gene64658:CDS 40.0%
TGTAAAAGGGCCCAAAGTTT+CGG + chr5.2:67782291-67782310 None:intergenic 40.0%
TTAGAGATTTAGTGAGGGTG+TGG - chr5.2:67780929-67780948 MS.gene64658:intron 40.0%
! AGACCAATTAGCGTGTTGTT+TGG + chr5.2:67778802-67778821 None:intergenic 40.0%
! AGCGTACAAGTGTGAGATTT+TGG + chr5.2:67779031-67779050 None:intergenic 40.0%
! CATAGCGGAATTTGTGCAAA+CGG - chr5.2:67781162-67781181 MS.gene64658:intron 40.0%
! GTTTGGTTCAATGTCGTCAA+TGG + chr5.2:67780120-67780139 None:intergenic 40.0%
! TGCGATGACCAATTTTGTGA+AGG - chr5.2:67780028-67780047 MS.gene64658:intron 40.0%
!! AATTTCTTTATGGAGTGCCG+TGG - chr5.2:67780811-67780830 MS.gene64658:intron 40.0%
!! ATGCTGTTGGTTCCTATGAA+AGG - chr5.2:67781481-67781500 MS.gene64658:intron 40.0%
!! TGCTGTTGGTTCCTATGAAA+GGG - chr5.2:67781482-67781501 MS.gene64658:intron 40.0%
!! TGGATGTATGTTTTTGCAGG+GGG - chr5.2:67782351-67782370 MS.gene64658:intron 40.0%
!! TGGTTTTCAAGCTGATGTCA+AGG - chr5.2:67779174-67779193 MS.gene64658:CDS 40.0%
!!! TTTTGGAGTAATCACGAGTG+AGG + chr5.2:67779014-67779033 None:intergenic 40.0%
ACACACCCCTTTCCTAATTC+AGG + chr5.2:67781450-67781469 None:intergenic 45.0%
ACGAGTGAGGATGTTGTAAC+CGG + chr5.2:67779001-67779020 None:intergenic 45.0%
ACTGCTTCTGTTTCAGACAG+TGG + chr5.2:67782008-67782027 None:intergenic 45.0%
AGTGTCCAACATGGCTTACT+CGG + chr5.2:67779067-67779086 None:intergenic 45.0%
ATTTCGGCTGCCGAAACTTT+GGG - chr5.2:67782278-67782297 MS.gene64658:CDS 45.0%
CAGGGCTGAGAAATGTGTTA+TGG - chr5.2:67779144-67779163 MS.gene64658:intron 45.0%
CCTCTTGAGTCCCTCTTATA+AGG - chr5.2:67782220-67782239 MS.gene64658:CDS 45.0%
CCTTATAAGAGGGACTCAAG+AGG + chr5.2:67782223-67782242 None:intergenic 45.0%
CGCACCATCCTTCACAAAAT+TGG + chr5.2:67780039-67780058 None:intergenic 45.0%
CTTGAGTCCCTCTTATAAGG+CGG - chr5.2:67782223-67782242 MS.gene64658:CDS 45.0%
GAAAGTAACTGAGCCATTGC+AGG + chr5.2:67781284-67781303 None:intergenic 45.0%
GATTTCAGCCGCCTTATAAG+AGG + chr5.2:67782234-67782253 None:intergenic 45.0%
GCTATATCCCACCCTTTCAT+AGG + chr5.2:67781496-67781515 None:intergenic 45.0%
GGATATAGCTCTCAAGGTTC+TGG - chr5.2:67781507-67781526 MS.gene64658:intron 45.0%
GTTGTCAAATATGGCCATGG+AGG - chr5.2:67779979-67779998 MS.gene64658:intron 45.0%
TAGCGTGAATGCATGATGAC+AGG + chr5.2:67782113-67782132 None:intergenic 45.0%
TATTTCGGCTGCCGAAACTT+TGG - chr5.2:67782277-67782296 MS.gene64658:CDS 45.0%
TGATGTCAAGGCTTTGCAGA+AGG - chr5.2:67779186-67779205 MS.gene64658:CDS 45.0%
TGGTTCAATGTCGTCAATGG+CGG + chr5.2:67780117-67780136 None:intergenic 45.0%
TTTACGAACTTCACCTGTGC+AGG + chr5.2:67781594-67781613 None:intergenic 45.0%
! GTTGGTTCCTATGAAAGGGT+GGG - chr5.2:67781486-67781505 MS.gene64658:intron 45.0%
!! AGGATGACTTGGTGTGAGTT+TGG - chr5.2:67779662-67779681 MS.gene64658:CDS 45.0%
!! ATACAATGTTGATGACCGCG+TGG - chr5.2:67780140-67780159 MS.gene64658:intron 45.0%
!! GGTTTTGTCTGCTAGGCATT+TGG - chr5.2:67779207-67779226 MS.gene64658:CDS 45.0%
!! TGCAGAAGGTTTTGTCTGCT+AGG - chr5.2:67779200-67779219 MS.gene64658:CDS 45.0%
!! TGTTGGTTCCTATGAAAGGG+TGG - chr5.2:67781485-67781504 MS.gene64658:intron 45.0%
!!! CATTGGTTTGGTTCCGCTTT+TGG + chr5.2:67779960-67779979 None:intergenic 45.0%
!!! ACTTTATTTTTTTTTTTTTT+TGG - chr5.2:67780710-67780729 MS.gene64658:intron 5.0%
ACTTCACCTGTGCAGGTAAG+AGG + chr5.2:67781587-67781606 None:intergenic 50.0%
AGGGTGGGATATAGCTCTCA+AGG - chr5.2:67781501-67781520 MS.gene64658:intron 50.0%
ATAGTGCACCTTCACTGTCG+AGG + chr5.2:67781364-67781383 None:intergenic 50.0%
ATGGCCATGGAGGATATAGG+TGG - chr5.2:67779989-67780008 MS.gene64658:intron 50.0%
CGATTACTGTGTTATCGCCG+CGG - chr5.2:67778960-67778979 MS.gene64658:intron 50.0%
CGCGGCGATAACACAGTAAT+CGG + chr5.2:67778962-67778981 None:intergenic 50.0%
CTCATCCGAGTAAGCCATGT+TGG - chr5.2:67779059-67779078 MS.gene64658:intron 50.0%
TGGCTGACATATCACGTCGT+GGG + chr5.2:67780063-67780082 None:intergenic 50.0%
!! GTGGACTGGGAGACTTTAGT+TGG + chr5.2:67781563-67781582 None:intergenic 50.0%
!! TATGGAGTGCCGTGGTTGAT+TGG - chr5.2:67780819-67780838 MS.gene64658:intron 50.0%
ACCTGTGCAGGTAAGAGGAG+TGG + chr5.2:67781582-67781601 None:intergenic 55.0%
CCATGGAGGATATAGGTGGC+GGG - chr5.2:67779993-67780012 MS.gene64658:intron 55.0%
CTGGCTGACATATCACGTCG+TGG + chr5.2:67780064-67780083 None:intergenic 55.0%
GCAAATGAGCTGCCCTGCAA+TGG - chr5.2:67781268-67781287 MS.gene64658:intron 55.0%
GCCATGGAGGATATAGGTGG+CGG - chr5.2:67779992-67780011 MS.gene64658:intron 55.0%
GCGGATACGAGGATGTCTAC+CGG - chr5.2:67778979-67778998 MS.gene64658:intron 55.0%
GGTAGACATCCTCGTATCCG+CGG + chr5.2:67778980-67778999 None:intergenic 55.0%
TAGAACATGGGAGCCGCAGA+AGG + chr5.2:67780980-67780999 None:intergenic 55.0%
TCCACTCCTCTTACCTGCAC+AGG - chr5.2:67781578-67781597 MS.gene64658:intron 55.0%
TGCAGGTAAGAGGAGTGGAC+TGG + chr5.2:67781577-67781596 None:intergenic 55.0%
! GCAGGTAAGAGGAGTGGACT+GGG + chr5.2:67781576-67781595 None:intergenic 55.0%
!! ATGTCGTCAATGGCGGACAG+TGG + chr5.2:67780110-67780129 None:intergenic 55.0%
!! ATGTTGATGACCGCGTGGCA+AGG - chr5.2:67780145-67780164 MS.gene64658:intron 55.0%
!! GTTGATGACTCCTTGCCACG+CGG + chr5.2:67780158-67780177 None:intergenic 55.0%
CCCGCCACCTATATCCTCCA+TGG + chr5.2:67779996-67780015 None:intergenic 60.0%
GTGTTATCGCCGCGGATACG+AGG - chr5.2:67778968-67778987 MS.gene64658:intron 60.0%
!! TCGTCAATGGCGGACAGTGG+CGG + chr5.2:67780107-67780126 None:intergenic 60.0%
TTGGGCGGCCTCGACAGTGA+AGG - chr5.2:67781353-67781372 MS.gene64658:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 67778795 67782444 67778795 ID=MS.gene64658
chr5.2 mRNA 67778795 67782444 67778795 ID=MS.gene64658.t1;Parent=MS.gene64658
chr5.2 exon 67782398 67782444 67782398 ID=MS.gene64658.t1.exon1;Parent=MS.gene64658.t1
chr5.2 CDS 67782398 67782444 67782398 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67782196 67782303 67782196 ID=MS.gene64658.t1.exon2;Parent=MS.gene64658.t1
chr5.2 CDS 67782196 67782303 67782196 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67782011 67782092 67782011 ID=MS.gene64658.t1.exon3;Parent=MS.gene64658.t1
chr5.2 CDS 67782011 67782092 67782011 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67779865 67779994 67779865 ID=MS.gene64658.t1.exon4;Parent=MS.gene64658.t1
chr5.2 CDS 67779865 67779994 67779865 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67779640 67779782 67779640 ID=MS.gene64658.t1.exon5;Parent=MS.gene64658.t1
chr5.2 CDS 67779640 67779782 67779640 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67779160 67779246 67779160 ID=MS.gene64658.t1.exon6;Parent=MS.gene64658.t1
chr5.2 CDS 67779160 67779246 67779160 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
chr5.2 exon 67778795 67778869 67778795 ID=MS.gene64658.t1.exon7;Parent=MS.gene64658.t1
chr5.2 CDS 67778795 67778869 67778795 ID=cds.MS.gene64658.t1;Parent=MS.gene64658.t1
Gene Sequence

>MS.gene64658

ATGACCAAACAACACGCTAATTGGTCTCCATATGACAACAATGGAGGGTAGGGTACTCTTTCTTTCTCTATTCTCAGTTTAAACAACTATTTTTTCAATAATTTTTTGGTATTAATTTCATTATTTGTTTGTATTTTGTAGATCTTGTGTTGCAATTGCTGGATCCGATTACTGTGTTATCGCCGCGGATACGAGGATGTCTACCGGTTACAACATCCTCACTCGTGATTACTCCAAAATCTCACACTTGTACGCTATTTATTTCTCATCCGAGTAAGCCATGTTGGACACTGTTTTTTTTTTTATGTTGTTGTTGTTGTTTAAGGTTGTTTTTTTGTTTGTTTTGTGACAGGGCTGAGAAATGTGTTATGGCTTCTTCTGGTTTTCAAGCTGATGTCAAGGCTTTGCAGAAGGTTTTGTCTGCTAGGCATTTGGTCAGTCTAACTTTCTTAATCTCATTGCTTTATCATCTAAATACTGTATTTGCAGCTTACACATGATAAGCGCTTATGTTTAAGCTTACATAAGCTATTTTTAAAGAAAAAGATAAAATAATGTTAATTTGTTTTTGTATATGCTATAAGTTGTTTTTGTATAAGGTATATTGGAAAACTTATGAAAATAAGTTGAAAAAAGGTTATGAAAATTGTCATAAGCTCTTTTCATAAATTCTCCCAAAACAGTATCACAAAATTTATGCGAGTAATTAAGCTCAAATAAGCGATTTCAAACTGGCCCTAAGTAGTACTATTTAGATTGCTAGTAGTCAAGCTCAAATAGTGTCACAAAACATAAGCAAGTAGGTAAGCTCAAATAAGCGATTCCAAACAGGCCCTAAGTTATACTATTTAGATTGCACATATTTTCAGGATGACTTGGTGTGAGTTTGGTTCTGCAGTGACGCAAATTAATTTTCAATGGATTGATTATGTGAAACTATTTCTGGCTAAAAGTGAGTTGAATGTATAGTGATTTATGTTTGGAAACGTTTGTGCAAATGTGAGTTGAATAGTACATTCAAGGCTAATAATCAATTGTAGAAGCAAAAAATTACAAATTCTAGCTTTAAGTTAGAATCAATTTTGGAGATAATAAATTCTACTCGAGAACATCCGCAAATTCCAAAATTCATTCTACACATCTAGAATCTATTTTTGCTCTTCCAAAAGCGGAACCAAACCAATGTTGTCAAATATGGCCATGGAGGATATAGGTGGCGGGTTTTTGACTTTCTGCGATGACCAATTTTGTGAAGGATGGTGCGACCCACGACGTGATATGTCAGCCAGTATTTCAGATTTTTGGCTCTCCGCCACTGTCCGCCATTGACGACATTGAACCAAACATACAATGTTGATGACCGCGTGGCAAGGAGTCATCAACATTCCTTGGATATCTTTTTTGGTTAAGTTCGTGAGTTAGGAAGGAAACTTTGCTAGGTTTTAAATTGATTTTAATTAAGCTCCCTATTTGTAACATTACTGATGTACAGATGTAGAACTATAAAAGAATAAAATAGTACTCCTCTAGGGTTAAATGTTCCTTTGTTTTGAGTTTCAGATATTCTAACAAGAAACTAAAAACTCAATATTCTTAACTATACAAAGCAGGTGTTGAATATTTGCATTGGTGGTTTATTTATGGACTCTCATGGTAATCAGAAACCTTAATGTAATAGCCCTATAAGTAAGTTTGACTATACTAAGTACATTCTCAGAAACCAAAATCCTTAATATGATAGCCCTATAAGTTTAACTATACCAGAGTTATTCGATTATTTTGGTCAACCCTTTTTCGCAGTGTTTCTATGAGCTTCTACACACATTGCCAAACTCTTCGCCATTTTTCTATATAGAAGTTACCCTCAACATATAATTGTATATCCAAAATTGTAAAGAAAACCTTAGTCTGTATGACTTTATTTTTTTTTTTTTTTGGAAATATTATTTTGCAACCTAACATGTTTCTGAATTAGTTCAATATGCATTTTAAGATACATGAATAATTGAATTTATAAATTTCTTTATGGAGTGCCGTGGTTGATTGGTTTGATGATACTAGTTGAATCAATTATGAAAGCTTAATCTTTTTTTGATGTATATAACCTGATTAATTATGTAGTTAGAGATGTTAGTTAGAGATTTAGTGAGGGTGTGGCGTTTGACTCATAAATTATGCTATACCTTCTGCGGCTCCCATGTTCTAATTTTACCTTGACAAAAAATTGAAGCCTCTGGTTGAAATGACATTAGTGTTGTCAAATAGCGGCAATAGCCGCACTATCGAACAGATATTTGTACAAATTGCTATTGCTTTTAGTCCAAATTGTTGTCAAATAGTGGCTACAACAACGCTATAACACTGTAGCATAGCGGAATTTGTGCAAACGGCTATTTTCTGCAATTCATGATTGACTATACTGTATGACATCATATTGATTCTGAATGCAGACATATCAGCATCAACACAACAAGCAAATGAGCTGCCCTGCAATGGCTCAGTTACTTTCAAACACACTTTATTACAAACGATTCTTTCCTTATTATTCTTTCAATGTTTTGGGCGGCCTCGACAGTGAAGGTGCACTATTTCTACCTTTCTATTATTGTAATGTTCCATTTTTGTATTTGCTGACTCGAACTTCTACTTCAACCTGAATTAGGAAAGGGGTGTGTCTTCACATATGATGCTGTTGGTTCCTATGAAAGGGTGGGATATAGCTCTCAAGGTTCTGGATCCACACTCATTATGCCTTTTCTTGATAACCAACTAAAGTCTCCCAGTCCACTCCTCTTACCTGCACAGGTGAAGTTCGTAAATTGATTTGCTTGAAATATTTGATGCTTTAAGGGCTCGTTTGTTATCGATTATAAAAAAGTGATTTTGACATTCAATAATTTAGAGATACTTTTTTTGGAGATTTTTAAGGAAAACATAACTTGTTGTGTTTGTTTACTATAATGAAAATCACTTTTAAACAAATCATCTATAAAAATGATTTTCACGAGAACCTACTAAGAACAACTTCTCATTTGAAATAGTTATAAGATTTTTTTTCCTTCAGATTCTAATTTTTTTAATGAACTAATACAAACTTCCAAAAGTGATTATTATTTTATTTTAAGAAAAAAGATGTAGCAAACAGGCCCTAAACTGTGCTTAACTAACACCTCTACATTAAAATATGATTTATTGCAGGATGCTGTTACTCCACTGTCTGAAACAGAAGCAGTTGATTTGGTCAAAACAGTTTTTGCATCAGCAACTGAGAGAGATATATACACTGTAAGTTTGTTTGCATACATAAATGAATTGCCTGTCATCATGCATTCACGCTATTGCGGATCCAGACCGTTCAATCTTTATTAAACGAGGCACATTTTGGCTTTAGTAAAGACCTATTTTGCAAAGTCGAAGTACGAGTCCCTCTTGAGTCCCTCTTATAAGGCGGCTGAAATCTCATGATTGTGTGAATGCAGAGTTATTTCGGCTGCCGAAACTTTGGGCCCTTTTACATAAATGTCATTTTACATTAGGACAAATGTTTAACTTACTTTGGATGTATGTTTTTGCAGGGGGATAAAGTTGAAATTGTCATCCTAAATGCGAGTGGTATTCATCGTGAATTCATGGACCTAAGGAAAGACTGA
Protein sequence

>MS.gene64658.t1

MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSETEAVDLVKTVFASATERDIYTGDKVEIVILNASGIHREFMDLRKD