AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78385

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78385.t1 MTR_2g098010 100.000 223 0 0 1 223 1 223 1.19e-170 468
MS.gene78385.t1 MTR_5g079740 98.655 223 3 0 1 223 1 223 1.96e-168 462
MS.gene78385.t1 MTR_5g079740 98.193 166 3 0 58 223 1 166 5.57e-121 340
MS.gene78385.t1 MTR_7g088490 32.558 129 87 0 9 137 2 130 5.54e-18 79.3
MS.gene78385.t1 MTR_4g087680 32.283 127 86 0 11 137 4 130 9.44e-17 75.9
MS.gene78385.t1 MTR_3g109210 27.273 209 126 4 15 217 13 201 1.43e-15 73.2
MS.gene78385.t1 MTR_4g023520 94.118 34 2 0 58 91 1 34 5.91e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78385.t1 AT3G60820 87.444 223 28 0 1 223 1 223 2.77e-150 417
MS.gene78385.t1 AT3G60820 87.444 223 28 0 1 223 1 223 2.77e-150 417
MS.gene78385.t1 AT3G60820 86.547 223 30 0 1 223 1 223 2.62e-148 412
MS.gene78385.t1 AT1G21720 32.558 129 87 0 9 137 2 130 2.33e-18 80.5
MS.gene78385.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.70e-17 78.2
MS.gene78385.t1 AT1G77440 31.783 129 88 0 9 137 2 130 1.70e-17 78.2
MS.gene78385.t1 AT4G31300 26.794 209 127 4 15 217 12 200 6.04e-13 66.2
MS.gene78385.t1 AT4G31300 26.794 209 127 4 15 217 12 200 6.04e-13 66.2
MS.gene78385.t1 AT4G31300 24.878 205 137 3 15 217 12 201 5.88e-12 63.5

Find 54 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTACAGGTGAGAGATTT+TGG 0.141897 2.3:-5218733 None:intergenic
GGATATAGCTCTCAGGGTTC+TGG 0.295385 2.3:+5220773 MS.gene78385:CDS
TGACTAAACAACACGCTAAT+TGG 0.296923 2.3:+5218499 MS.gene78385:CDS
AAATGTGTGATGGCTTCTTC+TGG 0.302083 2.3:+5218969 MS.gene78385:CDS
TACTATTCTTTCAATGTGTT+GGG 0.329503 2.3:+5220600 MS.gene78385:CDS
GATGAATACCACCAGCATTT+AGG 0.333947 2.3:-5221349 None:intergenic
TTACTATTCTTTCAATGTGT+TGG 0.338506 2.3:+5220599 MS.gene78385:CDS
TACCCTCCATTGTTGTCATA+TGG 0.354246 2.3:-5218525 None:intergenic
AGATCTTGTGTCGCAATTGC+TGG 0.365009 2.3:+5218639 MS.gene78385:intron
GGCAGGTAAGAGGAGTGGGC+TGG 0.368090 2.3:-5220840 None:intergenic
CAGGTAAGAGGAGTGGGCTG+GGG 0.380698 2.3:-5220838 None:intergenic
GCAGGTAAGAGGAGTGGGCT+GGG 0.386622 2.3:-5220839 None:intergenic
AGGTAAGAGGAGTGGGCTGG+GGG 0.399364 2.3:-5220837 None:intergenic
GAAAGCAATTGAGCCATTGC+AGG 0.407579 2.3:-5220546 None:intergenic
ACTTCACCTGGGCAGGTAAG+AGG 0.436789 2.3:-5220850 None:intergenic
GCTATATCCCACCCTCTCAT+AGG 0.437293 2.3:-5220759 None:intergenic
CCCACTCCTCTTACCTGCCC+AGG 0.466732 2.3:+5220844 MS.gene78385:CDS
TTGTGTCGCAATTGCTGGAT+CGG 0.468844 2.3:+5218644 MS.gene78385:CDS
TTCAAGAACTTCACCTGGGC+AGG 0.478299 2.3:-5220857 None:intergenic
TTCAGTTGGTTATCAAGAAA+CGG 0.480345 2.3:-5220812 None:intergenic
TGAAGCAGAAGCAGTTGATC+TGG 0.483304 2.3:+5220980 MS.gene78385:CDS
TGGTATTCATCGTGAGTTCA+TGG 0.483816 2.3:+5221361 MS.gene78385:CDS
AAAAGCATCAGTCTTTCCTT+AGG 0.487279 2.3:-5221385 None:intergenic
GAGGGTGGGATATAGCTCTC+AGG 0.498734 2.3:+5220766 MS.gene78385:CDS
GCGAGTGAGGATGTTGTAAC+CGG 0.513102 2.3:-5218703 None:intergenic
GCAGATGAGCTGCCCTGCAA+TGG 0.526195 2.3:+5220533 MS.gene78385:CDS
AACACATTGAAAGAATAGTA+AGG 0.535290 2.3:-5220597 None:intergenic
CCTGGGCAGGTAAGAGGAGT+GGG 0.542041 2.3:-5220844 None:intergenic
GTCTTCACTTATGACGCTGT+TGG 0.550782 2.3:+5220734 MS.gene78385:CDS
TGCTTCAGACAGTGGAGTAA+CGG 0.553618 2.3:-5220964 None:intergenic
ATCGTGAGTTCATGGACCTA+AGG 0.553653 2.3:+5221369 MS.gene78385:CDS
TGTTGGTTCCTATGAGAGGG+TGG 0.564322 2.3:+5220751 MS.gene78385:CDS
TGATGTGAAGGCTTTGCAGA+AGG 0.566876 2.3:+5219001 MS.gene78385:CDS
ACCTGGGCAGGTAAGAGGAG+TGG 0.577674 2.3:-5220845 None:intergenic
CTCCATATGACAACAATGGA+GGG 0.584655 2.3:+5218523 MS.gene78385:CDS
GTGTGATCGCAGCTGATACA+AGG 0.586277 2.3:+5218673 MS.gene78385:CDS
ACGCTGTTGGTTCCTATGAG+AGG 0.586650 2.3:+5220747 MS.gene78385:CDS
TATTCTTTCAATGTGTTGGG+CGG 0.587319 2.3:+5220603 MS.gene78385:CDS
GAAATTGTCATCCTAAATGC+TGG 0.591622 2.3:+5221338 MS.gene78385:CDS
ATTAGCGTGTTGTTTAGTCA+TGG 0.591691 2.3:-5218496 None:intergenic
ACTGCTTCTGCTTCAGACAG+TGG 0.591728 2.3:-5220972 None:intergenic
AGGGTGGGATATAGCTCTCA+GGG 0.602013 2.3:+5220767 MS.gene78385:CDS
AAAGCAATTGAGCCATTGCA+GGG 0.602125 2.3:-5220545 None:intergenic
AAGAAACGGCATAATGAGAG+TGG 0.608965 2.3:-5220798 None:intergenic
CGCTGTTGGTTCCTATGAGA+GGG 0.609542 2.3:+5220748 MS.gene78385:CDS
ATAGCTCACCTTCACTGTCA+AGG 0.612785 2.3:-5220626 None:intergenic
GTTGGTTCCTATGAGAGGGT+GGG 0.623819 2.3:+5220752 MS.gene78385:CDS
AGTGAGGATGTTGTAACCGG+TGG 0.625722 2.3:-5218700 None:intergenic
TCTCCATATGACAACAATGG+AGG 0.635572 2.3:+5218522 MS.gene78385:CDS
TGGTCTCCATATGACAACAA+TGG 0.646174 2.3:+5218519 MS.gene78385:CDS
GCTGATACAAGGATGTCCAC+CGG 0.646826 2.3:+5218684 MS.gene78385:CDS
TTGGGCGGCCTTGACAGTGA+AGG 0.657648 2.3:+5220618 MS.gene78385:CDS
AAGAGCTGAGAAATGTGTGA+TGG 0.689435 2.3:+5218959 MS.gene78385:intron
ATTGTCATCCTAAATGCTGG+TGG 0.706189 2.3:+5221341 MS.gene78385:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACAAAACAAATAAAATT+AGG - chr2.3:5220017-5220036 None:intergenic 10.0%
!! ATTAAAATATGATTTATTGC+AGG + chr2.3:5220938-5220957 MS.gene78385:intron 15.0%
!! ATTGAAAGATATTAAAACAA+GGG - chr2.3:5218589-5218608 None:intergenic 15.0%
!! TATAAACTTTCTATAAATGT+GGG - chr2.3:5219992-5220011 None:intergenic 15.0%
!! TTATTTATTTATCTGTTACA+AGG - chr2.3:5219067-5219086 None:intergenic 15.0%
!!! TAAATCATATTTTAATGTAG+AGG - chr2.3:5220934-5220953 None:intergenic 15.0%
!!! TTATCATTTCTTTAGAATTT+TGG - chr2.3:5220103-5220122 None:intergenic 15.0%
!! AAAAATAGTCCAATAAGTAT+AGG - chr2.3:5219878-5219897 None:intergenic 20.0%
!! AAAATAAATATAGAGGTGAA+TGG - chr2.3:5219818-5219837 None:intergenic 20.0%
!! ACAATAATAGAAAACCAATT+GGG - chr2.3:5218813-5218832 None:intergenic 20.0%
!! ATAGAGTATGTGTATAAATT+AGG + chr2.3:5219585-5219604 MS.gene78385:intron 20.0%
!! CATAAACACAACTAAAAAAA+AGG - chr2.3:5218884-5218903 None:intergenic 20.0%
!! GAACATCAAAATAAATATAG+AGG - chr2.3:5219825-5219844 None:intergenic 20.0%
!! GATTGAAAGATATTAAAACA+AGG - chr2.3:5218590-5218609 None:intergenic 20.0%
!! GTATAAACTTTCTATAAATG+TGG - chr2.3:5219993-5220012 None:intergenic 20.0%
!! TAACTTCTATATAGAAAAGA+TGG - chr2.3:5219958-5219977 None:intergenic 20.0%
!! TACAATAATAGAAAACCAAT+TGG - chr2.3:5218814-5218833 None:intergenic 20.0%
!! TAGTTAGTTACATAGTTATT+TGG + chr2.3:5219555-5219574 MS.gene78385:intron 20.0%
!! TATAGAAACATTAGAAAAGA+GGG - chr2.3:5219906-5219925 None:intergenic 20.0%
!! TATTATTGTAAAACTTCTAG+TGG + chr2.3:5218824-5218843 MS.gene78385:intron 20.0%
!! TCAAAATTTACTATTGCTAT+TGG + chr2.3:5220163-5220182 MS.gene78385:intron 20.0%
!! TGTGTTACGATATATAATAA+TGG + chr2.3:5219506-5219525 MS.gene78385:intron 20.0%
!! TTATAGAAACATTAGAAAAG+AGG - chr2.3:5219907-5219926 None:intergenic 20.0%
!! TTGAAAGATATTAAAACAAG+GGG - chr2.3:5218588-5218607 None:intergenic 20.0%
!! TTTAGATTGTAAACATATTC+AGG + chr2.3:5219092-5219111 MS.gene78385:intron 20.0%
!! TTTGGATATACAATTATACT+TGG - chr2.3:5220085-5220104 None:intergenic 20.0%
!!! AAATATTTTCTAGATGAGTA+AGG + chr2.3:5219190-5219209 MS.gene78385:intron 20.0%
!!! AGTTTGTTTATGTATGAAAA+TGG + chr2.3:5221050-5221069 MS.gene78385:intron 20.0%
!!! TTAGATGTTAGTTTGAAATT+TGG + chr2.3:5220365-5220384 MS.gene78385:intron 20.0%
! AACACATTGAAAGAATAGTA+AGG - chr2.3:5220600-5220619 None:intergenic 25.0%
! AATATCGTAAAGTTTGTGAA+TGG + chr2.3:5219637-5219656 MS.gene78385:intron 25.0%
! AGTTATGTTAACAAATAGAG+AGG - chr2.3:5219257-5219276 None:intergenic 25.0%
! ATAGAAACATTAGAAAAGAG+GGG - chr2.3:5219905-5219924 None:intergenic 25.0%
! ATCTAACAACGTAATTGATA+AGG - chr2.3:5220351-5220370 None:intergenic 25.0%
! CAAAATGACAATTGTGTAAA+AGG - chr2.3:5221261-5221280 None:intergenic 25.0%
! CAAGGTAATATTCAAAACAA+TGG - chr2.3:5220439-5220458 None:intergenic 25.0%
! GGCTAATTAAATTAGTTAAG+AGG + chr2.3:5219527-5219546 MS.gene78385:intron 25.0%
! TAAACACTAAAGTCAAAATG+TGG - chr2.3:5221133-5221152 None:intergenic 25.0%
! TAACAAATAGAGAGGTTAAA+AGG - chr2.3:5219249-5219268 None:intergenic 25.0%
! TACTATTCTTTCAATGTGTT+GGG + chr2.3:5220600-5220619 MS.gene78385:CDS 25.0%
! TGAAAGATATTAAAACAAGG+GGG - chr2.3:5218587-5218606 None:intergenic 25.0%
! TTAAGAAAATAAGAAAGACC+AGG - chr2.3:5219711-5219730 None:intergenic 25.0%
! TTACTATTCTTTCAATGTGT+TGG + chr2.3:5220599-5220618 MS.gene78385:CDS 25.0%
!! ATTCTAAAGAAATGATAAGC+AGG + chr2.3:5220105-5220124 MS.gene78385:intron 25.0%
!! TTTGAAATTTGGTGAAATTG+TGG + chr2.3:5220376-5220395 MS.gene78385:intron 25.0%
!!! GTGTTTTTCTTGAAGTATTT+TGG + chr2.3:5220883-5220902 MS.gene78385:intron 25.0%
!!! GTTAAATGTTGCTTTGATTT+GGG + chr2.3:5219320-5219339 MS.gene78385:intron 25.0%
ACAAACAGAACAAAAACACA+AGG - chr2.3:5219142-5219161 None:intergenic 30.0%
ACTTCAACCTAAAATAGGAA+AGG + chr2.3:5220706-5220725 MS.gene78385:intron 30.0%
ATATATTGCTGAAACCCAAT+TGG + chr2.3:5218796-5218815 MS.gene78385:intron 30.0%
ATTCTATATACCGAGAAAAG+AGG - chr2.3:5219449-5219468 None:intergenic 30.0%
CAAATAAGATATACAGACTC+AGG - chr2.3:5220194-5220213 None:intergenic 30.0%
CCAAAAATATTGACATTCCA+AGG - chr2.3:5220246-5220265 None:intergenic 30.0%
CTGAGTCTGTATATCTTATT+TGG + chr2.3:5220192-5220211 MS.gene78385:intron 30.0%
CTTCAACCTAAAATAGGAAA+GGG + chr2.3:5220707-5220726 MS.gene78385:intron 30.0%
CTTCAACTTCAACCTAAAAT+AGG + chr2.3:5220701-5220720 MS.gene78385:intron 30.0%
GAAGCTTCAATTATTTGTCA+AGG - chr2.3:5220457-5220476 None:intergenic 30.0%
GTAACACATTATGTCATGAT+GGG - chr2.3:5219494-5219513 None:intergenic 30.0%
GTCCAATAAGTATAGGTAAA+AGG - chr2.3:5219871-5219890 None:intergenic 30.0%
TACATGACATGCATTATGTT+AGG - chr2.3:5219771-5219790 None:intergenic 30.0%
TATTCAAAACAATGGAAGCA+TGG - chr2.3:5220431-5220450 None:intergenic 30.0%
TCTATATAGAAAAGATGGCA+AGG - chr2.3:5219953-5219972 None:intergenic 30.0%
TGTAAACATATTCAGGAAAC+AGG + chr2.3:5219099-5219118 MS.gene78385:intron 30.0%
TTCAGTTGGTTATCAAGAAA+CGG - chr2.3:5220815-5220834 None:intergenic 30.0%
! ATAAATTAGGAGAGCACAAA+GGG + chr2.3:5219598-5219617 MS.gene78385:intron 30.0%
! CCTTGGAATGTCAATATTTT+TGG + chr2.3:5220243-5220262 MS.gene78385:intron 30.0%
! CTCCTTTTACCTATACTTAT+TGG + chr2.3:5219866-5219885 MS.gene78385:intron 30.0%
! TATAAATTAGGAGAGCACAA+AGG + chr2.3:5219597-5219616 MS.gene78385:intron 30.0%
! TCACTATGTTGATTACTTGT+TGG + chr2.3:5219167-5219186 MS.gene78385:intron 30.0%
! TGAGTCTAAACTAGGTTTTT+GGG + chr2.3:5218918-5218937 MS.gene78385:intron 30.0%
!! ACAAGCTTTAAACTTGCTTT+GGG + chr2.3:5221288-5221307 MS.gene78385:intron 30.0%
!! GGTTAAATGTTGCTTTGATT+TGG + chr2.3:5219319-5219338 MS.gene78385:intron 30.0%
!! TTCTGCACTAGTATTTTTGT+TGG + chr2.3:5219842-5219861 MS.gene78385:intron 30.0%
!! TTTGGGTGTATGTTTTTACA+GGG + chr2.3:5221305-5221324 MS.gene78385:intron 30.0%
!!! CACAATTGTCATTTTGCATT+AGG + chr2.3:5221265-5221284 MS.gene78385:intron 30.0%
!!! GTATTTTGACTTTGCAAAAC+AGG - chr2.3:5221159-5221178 None:intergenic 30.0%
AAAGCAACATTTAACCCTAG+AGG - chr2.3:5219315-5219334 None:intergenic 35.0%
AAATGATAAGCAGGATTGAG+GGG + chr2.3:5220114-5220133 MS.gene78385:intron 35.0%
AACATATTCAGGAAACAGGA+TGG + chr2.3:5219103-5219122 MS.gene78385:intron 35.0%
AGAAATGATAAGCAGGATTG+AGG + chr2.3:5220112-5220131 MS.gene78385:intron 35.0%
AGAGCGAGCAAATACAAAAA+TGG - chr2.3:5220679-5220698 None:intergenic 35.0%
AGTAGCAACTCTAATGCAAA+GGG + chr2.3:5219660-5219679 MS.gene78385:intron 35.0%
ATACACACATGAAGTTGTGT+AGG - chr2.3:5218768-5218787 None:intergenic 35.0%
ATTGAGGGGAAAGTAAAAAC+TGG + chr2.3:5220128-5220147 MS.gene78385:intron 35.0%
ATTTCGACTGCAGAAAATTG+TGG + chr2.3:5221234-5221253 MS.gene78385:intron 35.0%
CGTAACACATTATGTCATGA+TGG - chr2.3:5219495-5219514 None:intergenic 35.0%
GAAATGATAAGCAGGATTGA+GGG + chr2.3:5220113-5220132 MS.gene78385:intron 35.0%
GAAATTGTCATCCTAAATGC+TGG + chr2.3:5221338-5221357 MS.gene78385:CDS 35.0%
GAGCGAGCAAATACAAAAAT+GGG - chr2.3:5220678-5220697 None:intergenic 35.0%
TAGGATGAGAAATAGCTTAC+AGG - chr2.3:5218749-5218768 None:intergenic 35.0%
TATTCTTTCAATGTGTTGGG+CGG + chr2.3:5220603-5220622 MS.gene78385:CDS 35.0%
TGACTAAACAACACGCTAAT+TGG + chr2.3:5218499-5218518 MS.gene78385:CDS 35.0%
TTCTATTCCATTCAGCTCTT+GGG + chr2.3:5219743-5219762 MS.gene78385:intron 35.0%
TTTCTATTCCATTCAGCTCT+TGG + chr2.3:5219742-5219761 MS.gene78385:intron 35.0%
! AAACTCAGAGTATTCCTCTA+GGG + chr2.3:5219298-5219317 MS.gene78385:intron 35.0%
! ACACATCCCTTTCCTATTTT+AGG - chr2.3:5220716-5220735 None:intergenic 35.0%
! ATACCGAGAAAAGAGGTTTT+CGG - chr2.3:5219442-5219461 None:intergenic 35.0%
! ATGTCATGTATTGAGTTGTG+AGG + chr2.3:5219781-5219800 MS.gene78385:intron 35.0%
! CTGAGTCTAAACTAGGTTTT+TGG + chr2.3:5218917-5218936 MS.gene78385:intron 35.0%
! GAGTCTAAACTAGGTTTTTG+GGG + chr2.3:5218919-5218938 MS.gene78385:intron 35.0%
! TAAACTCAGAGTATTCCTCT+AGG + chr2.3:5219297-5219316 MS.gene78385:intron 35.0%
! TAACCGAAAACCTCTTTTCT+CGG + chr2.3:5219436-5219455 MS.gene78385:intron 35.0%
!! CTTTGGGTGTATGTTTTTAC+AGG + chr2.3:5221304-5221323 MS.gene78385:intron 35.0%
!! GACAAGCTTTAAACTTGCTT+TGG + chr2.3:5221287-5221306 MS.gene78385:intron 35.0%
!! TATGTTTTTACAGGGGGATA+AGG + chr2.3:5221313-5221332 MS.gene78385:intron 35.0%
!! TTGGGTGTATGTTTTTACAG+GGG + chr2.3:5221306-5221325 MS.gene78385:intron 35.0%
AAAGCAATTGAGCCATTGCA+GGG - chr2.3:5220548-5220567 None:intergenic 40.0%
AAATGTGTGATGGCTTCTTC+TGG + chr2.3:5218969-5218988 MS.gene78385:CDS 40.0%
AAGAAACGGCATAATGAGAG+TGG - chr2.3:5220801-5220820 None:intergenic 40.0%
AAGAGCTGAGAAATGTGTGA+TGG + chr2.3:5218959-5218978 MS.gene78385:intron 40.0%
ACCAATTCAAGAACTTCACC+TGG - chr2.3:5220865-5220884 None:intergenic 40.0%
AGGATCGTGGACATGTTTAA+AGG - chr2.3:5219933-5219952 None:intergenic 40.0%
ATTAAAACAAGGGGGAGAGA+GGG - chr2.3:5218579-5218598 None:intergenic 40.0%
ATTGTCATCCTAAATGCTGG+TGG + chr2.3:5221341-5221360 MS.gene78385:CDS 40.0%
CACATTCACACTTGCAGAAT+TGG + chr2.3:5221087-5221106 MS.gene78385:intron 40.0%
CCAATTCAAGAACTTCACCT+GGG - chr2.3:5220864-5220883 None:intergenic 40.0%
CTCCATATGACAACAATGGA+GGG + chr2.3:5218523-5218542 MS.gene78385:CDS 40.0%
GAAGATGCTGAGTCTAAACT+AGG + chr2.3:5218910-5218929 MS.gene78385:intron 40.0%
GAGTAGCAACTCTAATGCAA+AGG + chr2.3:5219659-5219678 MS.gene78385:intron 40.0%
GATGAATACCACCAGCATTT+AGG - chr2.3:5221352-5221371 None:intergenic 40.0%
GGTTTATTTATAGACCGCCA+TGG + chr2.3:5219413-5219432 MS.gene78385:intron 40.0%
GTAGCAACTCTAATGCAAAG+GGG + chr2.3:5219661-5219680 MS.gene78385:intron 40.0%
TAATGCAAAGGGGAAACTCT+TGG + chr2.3:5219671-5219690 MS.gene78385:intron 40.0%
TACCCTCCATTGTTGTCATA+TGG - chr2.3:5218528-5218547 None:intergenic 40.0%
TATTAAAACAAGGGGGAGAG+AGG - chr2.3:5218580-5218599 None:intergenic 40.0%
TCTCCATATGACAACAATGG+AGG + chr2.3:5218522-5218541 MS.gene78385:CDS 40.0%
TGGTATTCATCGTGAGTTCA+TGG + chr2.3:5221361-5221380 MS.gene78385:CDS 40.0%
TGGTCTCCATATGACAACAA+TGG + chr2.3:5218519-5218538 MS.gene78385:CDS 40.0%
TTCAACCGCCTGATCTTAAT+AGG - chr2.3:5221189-5221208 None:intergenic 40.0%
! AAAGAGGTTTTCGGTTACCA+TGG - chr2.3:5219433-5219452 None:intergenic 40.0%
! AAGCAGTGTTGAGCATTTGA+TGG + chr2.3:5219392-5219411 MS.gene78385:intron 40.0%
! AGCTTACAGGTGAGAGATTT+TGG - chr2.3:5218736-5218755 None:intergenic 40.0%
! CAAAACAATGGAAGCATGGA+AGG - chr2.3:5220427-5220446 None:intergenic 40.0%
!! TGGGTGTATGTTTTTACAGG+GGG + chr2.3:5221307-5221326 MS.gene78385:intron 40.0%
!! TGGTTTTCAAGCTGATGTGA+AGG + chr2.3:5218989-5219008 MS.gene78385:CDS 40.0%
AATCTCGTGACTGTGAATGC+AGG + chr2.3:5221208-5221227 MS.gene78385:intron 45.0%
ACCGCCTGATCTTAATAGGA+CGG - chr2.3:5221185-5221204 None:intergenic 45.0%
AGAAAAGATGGCAAGGATCG+TGG - chr2.3:5219946-5219965 None:intergenic 45.0%
AGATCTTGTGTCGCAATTGC+TGG + chr2.3:5218639-5218658 MS.gene78385:intron 45.0%
ATAGCTCACCTTCACTGTCA+AGG - chr2.3:5220629-5220648 None:intergenic 45.0%
ATCGTGAGTTCATGGACCTA+AGG + chr2.3:5221369-5221388 MS.gene78385:CDS 45.0%
ATCTCGTGACTGTGAATGCA+GGG + chr2.3:5221209-5221228 MS.gene78385:intron 45.0%
CAAGCCGTCCTATTAAGATC+AGG + chr2.3:5221178-5221197 MS.gene78385:intron 45.0%
CCCAGGTGAAGTTCTTGAAT+TGG + chr2.3:5220861-5220880 MS.gene78385:intron 45.0%
CTGCAAGTGTGAATGTGTGA+TGG - chr2.3:5221084-5221103 None:intergenic 45.0%
CTTAGCAACATGACATGCCT+TGG + chr2.3:5220226-5220245 MS.gene78385:intron 45.0%
GAAAGCAATTGAGCCATTGC+AGG - chr2.3:5220549-5220568 None:intergenic 45.0%
GTCTTCACTTATGACGCTGT+TGG + chr2.3:5220734-5220753 MS.gene78385:CDS 45.0%
TGAAGCAGAAGCAGTTGATC+TGG + chr2.3:5220980-5220999 MS.gene78385:CDS 45.0%
TGATGTGAAGGCTTTGCAGA+AGG + chr2.3:5219001-5219020 MS.gene78385:CDS 45.0%
TGCTTCAGACAGTGGAGTAA+CGG - chr2.3:5220967-5220986 None:intergenic 45.0%
TTAGGAACCCAAGAGCTGAA+TGG - chr2.3:5219753-5219772 None:intergenic 45.0%
TTGGAGGAGAGATTATCTCC+TGG + chr2.3:5219690-5219709 MS.gene78385:intron 45.0%
TTGTGTCGCAATTGCTGGAT+CGG + chr2.3:5218644-5218663 MS.gene78385:CDS 45.0%
!! GGTTTTGTCTGCTAGGCATT+TGG + chr2.3:5219022-5219041 MS.gene78385:CDS 45.0%
!! TGCAGAAGGTTTTGTCTGCT+AGG + chr2.3:5219015-5219034 MS.gene78385:CDS 45.0%
!!! TTTTGGAGTAATCGCGAGTG+AGG - chr2.3:5218719-5218738 None:intergenic 45.0%
ACTGCTTCTGCTTCAGACAG+TGG - chr2.3:5220975-5220994 None:intergenic 50.0%
AGGGTGGGATATAGCTCTCA+GGG + chr2.3:5220767-5220786 MS.gene78385:CDS 50.0%
AGTGAGGATGTTGTAACCGG+TGG - chr2.3:5218703-5218722 None:intergenic 50.0%
GCCGTCCTATTAAGATCAGG+CGG + chr2.3:5221181-5221200 MS.gene78385:intron 50.0%
GCGAGTGAGGATGTTGTAAC+CGG - chr2.3:5218706-5218725 None:intergenic 50.0%
GCTATATCCCACCCTCTCAT+AGG - chr2.3:5220762-5220781 None:intergenic 50.0%
GCTGATACAAGGATGTCCAC+CGG + chr2.3:5218684-5218703 MS.gene78385:CDS 50.0%
GGATATAGCTCTCAGGGTTC+TGG + chr2.3:5220773-5220792 MS.gene78385:CDS 50.0%
GTGTGATCGCAGCTGATACA+AGG + chr2.3:5218673-5218692 MS.gene78385:CDS 50.0%
TGCAAAGGGGAAACTCTTGG+AGG + chr2.3:5219674-5219693 MS.gene78385:intron 50.0%
TTCAAGAACTTCACCTGGGC+AGG - chr2.3:5220860-5220879 None:intergenic 50.0%
! GAGGTTTTCGGTTACCATGG+CGG - chr2.3:5219430-5219449 None:intergenic 50.0%
! GTTGGTTCCTATGAGAGGGT+GGG + chr2.3:5220752-5220771 MS.gene78385:CDS 50.0%
!! ACGCTGTTGGTTCCTATGAG+AGG + chr2.3:5220747-5220766 MS.gene78385:CDS 50.0%
!! CGCTGTTGGTTCCTATGAGA+GGG + chr2.3:5220748-5220767 MS.gene78385:CDS 50.0%
!! TGTTGGTTCCTATGAGAGGG+TGG + chr2.3:5220751-5220770 MS.gene78385:CDS 50.0%
ACTTCACCTGGGCAGGTAAG+AGG - chr2.3:5220853-5220872 None:intergenic 55.0%
GAGGGTGGGATATAGCTCTC+AGG + chr2.3:5220766-5220785 MS.gene78385:CDS 55.0%
AACAAGGGGGAGAGAGGGAG+AGG - chr2.3:5218574-5218593 None:intergenic 60.0%
ACAAGGGGGAGAGAGGGAGA+GGG - chr2.3:5218573-5218592 None:intergenic 60.0%
ACCTGGGCAGGTAAGAGGAG+TGG - chr2.3:5220848-5220867 None:intergenic 60.0%
CCTGGGCAGGTAAGAGGAGT+GGG - chr2.3:5220847-5220866 None:intergenic 60.0%
GCAGATGAGCTGCCCTGCAA+TGG + chr2.3:5220533-5220552 MS.gene78385:CDS 60.0%
TTGGGCGGCCTTGACAGTGA+AGG + chr2.3:5220618-5220637 MS.gene78385:CDS 60.0%
! GCAGGTAAGAGGAGTGGGCT+GGG - chr2.3:5220842-5220861 None:intergenic 60.0%
!! AGGTAAGAGGAGTGGGCTGG+GGG - chr2.3:5220840-5220859 None:intergenic 60.0%
!! CAGGTAAGAGGAGTGGGCTG+GGG - chr2.3:5220841-5220860 None:intergenic 60.0%
CCCACTCCTCTTACCTGCCC+AGG + chr2.3:5220844-5220863 MS.gene78385:CDS 65.0%
GGCAGGTAAGAGGAGTGGGC+TGG - chr2.3:5220843-5220862 None:intergenic 65.0%
!! GTGGGCTGGGGGACTTCAGT+TGG - chr2.3:5220829-5220848 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5218498 5221400 5218498 ID=MS.gene78385
chr2.3 mRNA 5218498 5221400 5218498 ID=MS.gene78385.t1;Parent=MS.gene78385
chr2.3 exon 5218498 5218544 5218498 ID=MS.gene78385.t1.exon1;Parent=MS.gene78385.t1
chr2.3 CDS 5218498 5218544 5218498 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5218641 5218748 5218641 ID=MS.gene78385.t1.exon2;Parent=MS.gene78385.t1
chr2.3 CDS 5218641 5218748 5218641 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5218962 5219043 5218962 ID=MS.gene78385.t1.exon3;Parent=MS.gene78385.t1
chr2.3 CDS 5218962 5219043 5218962 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5220510 5220639 5220510 ID=MS.gene78385.t1.exon4;Parent=MS.gene78385.t1
chr2.3 CDS 5220510 5220639 5220510 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5220723 5220865 5220723 ID=MS.gene78385.t1.exon5;Parent=MS.gene78385.t1
chr2.3 CDS 5220723 5220865 5220723 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5220960 5221046 5220960 ID=MS.gene78385.t1.exon6;Parent=MS.gene78385.t1
chr2.3 CDS 5220960 5221046 5220960 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
chr2.3 exon 5221326 5221400 5221326 ID=MS.gene78385.t1.exon7;Parent=MS.gene78385.t1
chr2.3 CDS 5221326 5221400 5221326 ID=cds.MS.gene78385.t1;Parent=MS.gene78385.t1
Gene Sequence

>MS.gene78385

ATGACTAAACAACACGCTAATTGGTCTCCATATGACAACAATGGAGGGTAATCTCTCTCTCTCTCTCTCTCTCCCTCTCCCTCTCTCCCCCTTGTTTTAATATCTTTCAATCACTATCTTTTTAATATTATTATTATTTGTAGATCTTGTGTCGCAATTGCTGGATCGGATTACTGTGTGATCGCAGCTGATACAAGGATGTCCACCGGTTACAACATCCTCACTCGCGATTACTCCAAAATCTCTCACCTGTAAGCTATTTCTCATCCTACACAACTTCATGTGTGTATATATATATATATATTGCTGAAACCCAATTGGTTTTCTATTATTGTAAAACTTCTAGTGGTGTTAATTTTCTTGATCATAGCATTAGACAAATGCCTTTTTTTTAGTTGTGTTTATGCTGTTAGAAGATGCTGAGTCTAAACTAGGTTTTTGGGGTTTGTGTTTTGTTTCGCAAGAGCTGAGAAATGTGTGATGGCTTCTTCTGGTTTTCAAGCTGATGTGAAGGCTTTGCAGAAGGTTTTGTCTGCTAGGCATTTGGTGAGTCTAATAATCTCAATCCTTGTAACAGATAAATAAATAATACTATTTAGATTGTAAACATATTCAGGAAACAGGATGGCTTTGTTGAATTTCCTTGTGTTTTTGTTCTGTTTGTATGCTTCACTATGTTGATTACTTGTTGGAAATATTTTCTAGATGAGTAAGGAAGTTTAGAAGAAACTTTATTCAGTTGCAAATTCCTTTTAACCTCTCTATTTGTTAACATAACTGATTTAGAACTACAGAAGAATAAACTCAGAGTATTCCTCTAGGGTTAAATGTTGCTTTGATTTGGGTTTCAGATATTCTGATAATAAACTAAACTCAATATTCTTAGTTATACAAAAGCAGTGTTGAGCATTTGATGGTTTATTTATAGACCGCCATGGTAACCGAAAACCTCTTTTCTCGGTATATAGAATTAGATACATTGATTAATTGACACCCATCATGACATAATGTGTTACGATATATAATAATGGCTAATTAAATTAGTTAAGAGGTGATATAGTTAGTTACATAGTTATTTGGATTAGTTATAGAGTATGTGTATAAATTAGGAGAGCACAAAGGGTAGAGAGTATCTTTTAAATATCGTAAAGTTTGTGAATGGAGTAGCAACTCTAATGCAAAGGGGAAACTCTTGGAGGAGAGATTATCTCCTGGTCTTTCTTATTTTCTTAATTCAGTTGTTCTTTCTATTCCATTCAGCTCTTGGGTTCCTAACATAATGCATGTCATGTATTGAGTTGTGAGGATTGAGATCGACCATTCACCTCTATATTTATTTTGATGTTCTGCACTAGTATTTTTGTTGGTCTCCTTTTACCTATACTTATTGGACTATTTTTGTCACCCCTCTTTTCTAATGTTTCTATAAGTTCCTTTAAACATGTCCACGATCCTTGCCATCTTTTCTATATAGAAGTTATTGAGTTTAATCCCACATTTATAGAAAGTTTATACTCCTAATTTTATTTGTTTTGTATAATCATCATCTGCTATGTAAATTGTATTATTATCGTCTTTTTGACGCCAAGTATAATTGTATATCCAAAATTCTAAAGAAATGATAAGCAGGATTGAGGGGAAAGTAAAAACTGGCTAAGTGTTCTGTCAAAATTTACTATTGCTATTGGACTTTCCTGAGTCTGTATATCTTATTTGGAAATATTACTACTTAGCAACATGACATGCCTTGGAATGTCAATATTTTTGGAATACTGTGCTTAATTGATTTGCTGATACTAATTGCATTGCATCAATTATTAAAGTTTTTACTCTTTTATCAATGCATATAACCTTATCAATTACGTTGTTAGATGTTAGTTTGAAATTTGGTGAAATTGTGGTGTTTGACTCACAAACTGTTATTTACCTTCCATGCTTCCATTGTTTTGAATATTACCTTGACAAATAATTGAAGCTTCTGATTGAAATGACGCTATTGATTCTTAATGCAGACTTATCAGCATCAACACAACAAGCAGATGAGCTGCCCTGCAATGGCTCAATTGCTTTCAAACACACTTTATTACAAACGCTTCTTTCCTTACTATTCTTTCAATGTGTTGGGCGGCCTTGACAGTGAAGGTGAGCTATTTCTAACTTTCTATACTTGTTTGTGTCCCATTTTTGTATTTGCTCGCTCTGACTTCAACTTCAACCTAAAATAGGAAAGGGATGTGTCTTCACTTATGACGCTGTTGGTTCCTATGAGAGGGTGGGATATAGCTCTCAGGGTTCTGGCTCCACTCTCATTATGCCGTTTCTTGATAACCAACTGAAGTCCCCCAGCCCACTCCTCTTACCTGCCCAGGTGAAGTTCTTGAATTGGTGTTTTTCTTGAAGTATTTTGGTGCTTTAAATTATTTTTGAATAACACCTCTACATTAAAATATGATTTATTGCAGGATGCCGTTACTCCACTGTCTGAAGCAGAAGCAGTTGATCTGGTCAAAACTGTTTTCGCATCTGCAACTGAGAGAGATATATACACTGTAAGTTTGTTTATGTATGAAAATGGATTGTCTGCCATCACACATTCACACTTGCAGAATTGGATCGTCTAATCTTAATCAATCCACATTTTGACTTTAGTGTTTAAGACCTGTTTTGCAAAGTCAAAATACAAGCCGTCCTATTAAGATCAGGCGGTTGAAATCTCGTGACTGTGAATGCAGGGTTATTTCGACTGCAGAAAATTGTGGTCCTTTTACACAATTGTCATTTTGCATTAGGACAAGCTTTAAACTTGCTTTGGGTGTATGTTTTTACAGGGGGATAAGGTTGAAATTGTCATCCTAAATGCTGGTGGTATTCATCGTGAGTTCATGGACCTAAGGAAAGACTGA
Protein sequence

>MS.gene78385.t1

MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD