Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81653.t1 | XP_013454279.1 | 95.6 | 880 | 39 | 0 | 1 | 880 | 1 | 880 | 0.00E+00 | 1470.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81653.t1 | Q9C895 | 52.7 | 903 | 401 | 5 | 1 | 880 | 1 | 900 | 7.3e-217 | 755.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81653.t1 | A0A072UR29 | 95.6 | 880 | 39 | 0 | 1 | 880 | 1 | 880 | 0.0e+00 | 1470.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene81653 | 0.851133 | 1.08E-60 | -1.69E-46 |
| MS.gene049230 | MS.gene81653 | 0.833225 | 6.03E-56 | -1.69E-46 |
| MS.gene049378 | MS.gene81653 | 0.856447 | 3.20E-62 | -1.69E-46 |
| MS.gene049435 | MS.gene81653 | 0.818448 | 1.95E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene81653 | 0.803263 | 3.79E-49 | -1.69E-46 |
| MS.gene049647 | MS.gene81653 | 0.805278 | 1.44E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene81653 | 0.818787 | 1.63E-52 | -1.69E-46 |
| MS.gene049756 | MS.gene81653 | 0.802384 | 5.76E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene81653 | 0.856704 | 2.69E-62 | -1.69E-46 |
| MS.gene050227 | MS.gene81653 | 0.869601 | 2.74E-66 | -1.69E-46 |
| MS.gene050228 | MS.gene81653 | 0.872758 | 2.49E-67 | -1.69E-46 |
| MS.gene050442 | MS.gene81653 | 0.867069 | 1.80E-65 | -1.69E-46 |
| MS.gene050605 | MS.gene81653 | 0.83927 | 1.75E-57 | -1.69E-46 |
| MS.gene050623 | MS.gene81653 | -0.896659 | 2.98E-76 | -1.69E-46 |
| MS.gene050642 | MS.gene81653 | 0.837252 | 5.80E-57 | -1.69E-46 |
| MS.gene050719 | MS.gene81653 | 0.852195 | 5.41E-61 | -1.69E-46 |
| MS.gene050721 | MS.gene81653 | 0.85185 | 6.78E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene81653 | 0.840302 | 9.44E-58 | -1.69E-46 |
| MS.gene050759 | MS.gene81653 | 0.807243 | 5.56E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene81653 | 0.837495 | 5.03E-57 | -1.69E-46 |
| MS.gene050763 | MS.gene81653 | 0.837174 | 6.07E-57 | -1.69E-46 |
| MS.gene050780 | MS.gene81653 | 0.835805 | 1.35E-56 | -1.69E-46 |
| MS.gene050844 | MS.gene81653 | 0.801176 | 1.02E-48 | -1.69E-46 |
| MS.gene051210 | MS.gene81653 | 0.829708 | 4.43E-55 | -1.69E-46 |
| MS.gene051535 | MS.gene81653 | 0.805787 | 1.13E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene81653 | 0.819997 | 8.65E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene81653 | 0.850301 | 1.86E-60 | -1.69E-46 |
| MS.gene052012 | MS.gene81653 | 0.851542 | 8.29E-61 | -1.69E-46 |
| MS.gene052020 | MS.gene81653 | 0.801327 | 9.49E-49 | -1.69E-46 |
| MS.gene05215 | MS.gene81653 | 0.801445 | 8.97E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene81653 | 0.809734 | 1.63E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene81653 | 0.804511 | 2.09E-49 | -1.69E-46 |
| MS.gene052384 | MS.gene81653 | 0.806361 | 8.54E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene81653 | 0.819742 | 9.90E-53 | -1.69E-46 |
| MS.gene052896 | MS.gene81653 | 0.804837 | 1.78E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene81653 | 0.835447 | 1.67E-56 | -1.69E-46 |
| MS.gene053166 | MS.gene81653 | 0.809875 | 1.52E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene81653 | 0.822758 | 2.00E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene81653 | 0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene054379 | MS.gene81653 | 0.831184 | 1.93E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene81653 | 0.832855 | 7.45E-56 | -1.69E-46 |
| MS.gene054472 | MS.gene81653 | 0.814359 | 1.60E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene81653 | 0.808746 | 2.66E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene81653 | 0.813316 | 2.72E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene81653 | 0.815943 | 7.14E-52 | -1.69E-46 |
| MS.gene054658 | MS.gene81653 | 0.822234 | 2.64E-53 | -1.69E-46 |
| MS.gene05468 | MS.gene81653 | 0.821825 | 3.29E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene81653 | 0.812639 | 3.83E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene81653 | 0.843668 | 1.22E-58 | -1.69E-46 |
| MS.gene05517 | MS.gene81653 | 0.806923 | 6.50E-50 | -1.69E-46 |
| MS.gene055222 | MS.gene81653 | 0.810052 | 1.40E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene81653 | 0.807387 | 5.18E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene81653 | 0.850595 | 1.53E-60 | -1.69E-46 |
| MS.gene055701 | MS.gene81653 | 0.856926 | 2.31E-62 | -1.69E-46 |
| MS.gene055818 | MS.gene81653 | 0.814202 | 1.74E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene81653 | 0.809209 | 2.12E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene81653 | 0.829416 | 5.21E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene81653 | 0.844248 | 8.50E-59 | -1.69E-46 |
| MS.gene056552 | MS.gene81653 | 0.849885 | 2.43E-60 | -1.69E-46 |
| MS.gene056979 | MS.gene81653 | 0.842248 | 2.90E-58 | -1.69E-46 |
| MS.gene057026 | MS.gene81653 | 0.848344 | 6.51E-60 | -1.69E-46 |
| MS.gene057140 | MS.gene81653 | 0.813093 | 3.05E-51 | -1.69E-46 |
| MS.gene05761 | MS.gene81653 | 0.813971 | 1.95E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene81653 | 0.819308 | 1.24E-52 | -1.69E-46 |
| MS.gene058146 | MS.gene81653 | 0.852939 | 3.32E-61 | -1.69E-46 |
| MS.gene058399 | MS.gene81653 | 0.830459 | 2.90E-55 | -1.69E-46 |
| MS.gene058435 | MS.gene81653 | 0.813076 | 3.07E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene81653 | 0.82398 | 1.03E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene81653 | 0.803707 | 3.07E-49 | -1.69E-46 |
| MS.gene059161 | MS.gene81653 | 0.830732 | 2.49E-55 | -1.69E-46 |
| MS.gene059249 | MS.gene81653 | 0.822178 | 2.72E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene81653 | 0.800676 | 1.29E-48 | -1.69E-46 |
| MS.gene059609 | MS.gene81653 | 0.820432 | 6.88E-53 | -1.69E-46 |
| MS.gene059725 | MS.gene81653 | 0.829092 | 6.25E-55 | -1.69E-46 |
| MS.gene060084 | MS.gene81653 | 0.801345 | 9.41E-49 | -1.69E-46 |
| MS.gene060296 | MS.gene81653 | 0.811791 | 5.87E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene81653 | 0.809359 | 1.97E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene81653 | 0.834147 | 3.55E-56 | -1.69E-46 |
| MS.gene06052 | MS.gene81653 | 0.819777 | 9.72E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene81653 | 0.846298 | 2.37E-59 | -1.69E-46 |
| MS.gene060637 | MS.gene81653 | 0.820506 | 6.62E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene81653 | 0.844844 | 5.88E-59 | -1.69E-46 |
| MS.gene060685 | MS.gene81653 | 0.842601 | 2.34E-58 | -1.69E-46 |
| MS.gene060802 | MS.gene81653 | 0.853404 | 2.44E-61 | -1.69E-46 |
| MS.gene060803 | MS.gene81653 | 0.873004 | 2.06E-67 | -1.69E-46 |
| MS.gene060907 | MS.gene81653 | 0.800074 | 1.71E-48 | -1.69E-46 |
| MS.gene060942 | MS.gene81653 | 0.808645 | 2.80E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene81653 | 0.854023 | 1.62E-61 | -1.69E-46 |
| MS.gene061002 | MS.gene81653 | 0.806147 | 9.47E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene81653 | -0.816972 | 4.20E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81653.t1 | MTR_5g085010 | 96.705 | 880 | 29 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1732 |
| MS.gene81653.t1 | MTR_1g068830 | 89.773 | 880 | 90 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1500 |
| MS.gene81653.t1 | MTR_7g046250 | 31.172 | 879 | 597 | 6 | 4 | 880 | 5 | 877 | 4.85e-125 | 399 |
| MS.gene81653.t1 | MTR_7g046250 | 31.844 | 716 | 481 | 5 | 4 | 718 | 5 | 714 | 3.44e-96 | 317 |
| MS.gene81653.t1 | MTR_3g060950 | 73.203 | 153 | 24 | 3 | 711 | 861 | 61 | 198 | 2.28e-60 | 204 |
| MS.gene81653.t1 | MTR_5g065520 | 40.597 | 335 | 97 | 9 | 275 | 593 | 1 | 249 | 1.64e-46 | 172 |
| MS.gene81653.t1 | MTR_8g064230 | 82.143 | 84 | 15 | 0 | 252 | 335 | 2 | 85 | 2.55e-38 | 137 |
| MS.gene81653.t1 | MTR_5g065540 | 85.106 | 47 | 7 | 0 | 47 | 93 | 12 | 58 | 1.75e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81653.t1 | AT1G55250 | 54.649 | 882 | 397 | 3 | 1 | 880 | 1 | 881 | 0.0 | 922 |
| MS.gene81653.t1 | AT1G55250 | 53.437 | 902 | 395 | 6 | 1 | 880 | 1 | 899 | 0.0 | 905 |
| MS.gene81653.t1 | AT1G55250 | 53.156 | 903 | 397 | 5 | 1 | 880 | 1 | 900 | 0.0 | 900 |
| MS.gene81653.t1 | AT1G55250 | 58.421 | 380 | 157 | 1 | 502 | 880 | 4 | 383 | 1.08e-142 | 428 |
| MS.gene81653.t1 | AT1G55250 | 56.345 | 394 | 157 | 2 | 502 | 880 | 4 | 397 | 1.51e-138 | 418 |
| MS.gene81653.t1 | AT2G44950 | 30.499 | 882 | 595 | 11 | 8 | 880 | 6 | 878 | 2.57e-118 | 381 |
| MS.gene81653.t1 | AT2G44950 | 29.701 | 670 | 454 | 10 | 8 | 669 | 6 | 666 | 3.59e-74 | 257 |
Find 158 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCCTCCACAGAATCTTTC+TGG | 0.139617 | 5.1:+66928682 | None:intergenic |
| GATCTTGTATAGCCTGTTTC+TGG | 0.173211 | 5.1:+66929215 | None:intergenic |
| GAAGGAATTGAATTCTAAAT+TGG | 0.246983 | 5.1:-66923260 | MS.gene81653:CDS |
| CAGAATTCAGCTCAGCAATT+TGG | 0.247609 | 5.1:+66918495 | None:intergenic |
| GCTGAACAAGCTGCTGATTC+TGG | 0.251145 | 5.1:+66929248 | None:intergenic |
| TCAACATTGGATTGCTGAAT+TGG | 0.266671 | 5.1:-66923475 | MS.gene81653:CDS |
| TTTGTAGCTGGTTGATGATA+TGG | 0.269147 | 5.1:-66928948 | MS.gene81653:intron |
| CTTTGCGTCGGTTATCTACT+AGG | 0.292056 | 5.1:-66928617 | MS.gene81653:CDS |
| ACCTTTCATTTGCATCAAAC+TGG | 0.292093 | 5.1:+66920891 | None:intergenic |
| GTTACAGATTGAAAAGTTAC+AGG | 0.298097 | 5.1:-66921952 | MS.gene81653:intron |
| GATTGATGATATGACGAAAA+AGG | 0.308345 | 5.1:-66928345 | MS.gene81653:CDS |
| TCAGGAGTTAGAATTTGTTT+TGG | 0.321414 | 5.1:-66921922 | MS.gene81653:CDS |
| AAAAGATTCTTTGAAAACTT+TGG | 0.324595 | 5.1:-66928897 | MS.gene81653:CDS |
| AGCACTCGTCTATATAATTC+TGG | 0.335894 | 5.1:+66928255 | None:intergenic |
| TCTGACATAAAACAACTTAC+AGG | 0.344586 | 5.1:-66928221 | MS.gene81653:intron |
| TTCCCACCTGGCAAACTCTA+AGG | 0.353582 | 5.1:+66919289 | None:intergenic |
| TTTCTGAGCTTCAAGATTCC+CGG | 0.355151 | 5.1:-66923717 | MS.gene81653:CDS |
| CTTTCAGATTCTCTAACTTC+TGG | 0.356059 | 5.1:+66921058 | None:intergenic |
| CTCTAGACCATGTTCATCTA+AGG | 0.361421 | 5.1:+66921000 | None:intergenic |
| AAAGTTACAGGAGGAGAATC+AGG | 0.371139 | 5.1:-66921940 | MS.gene81653:CDS |
| AAGCTGAAATAGAAGAGTTA+AGG | 0.371256 | 5.1:-66920916 | MS.gene81653:CDS |
| AACCTCGATTTCAGATATAT+AGG | 0.375681 | 5.1:+66920480 | None:intergenic |
| CATCCCCATTTCTTTAGATA+AGG | 0.377185 | 5.1:+66922981 | None:intergenic |
| AGATGAGGCTTCTTCTTGTC+TGG | 0.380963 | 5.1:+66930320 | None:intergenic |
| GCTCTCTCTGCAGGCTTCTC+AGG | 0.381182 | 5.1:+66923883 | None:intergenic |
| CAAAGGATCAACTTCAACAT+TGG | 0.382751 | 5.1:-66923488 | MS.gene81653:CDS |
| TGCAAACAAATGTGCTGAAC+AGG | 0.382951 | 5.1:-66922246 | MS.gene81653:CDS |
| AAAGATATCAAAGCTGAGTT+TGG | 0.385405 | 5.1:-66923018 | MS.gene81653:CDS |
| TTCAGCCTTATCTAAAGAAA+TGG | 0.386599 | 5.1:-66922986 | MS.gene81653:CDS |
| TTCCTTTCCTTCGTGTCCTC+CGG | 0.387864 | 5.1:-66928730 | MS.gene81653:CDS |
| CAGGACTAACATTGCAAAAC+GGG | 0.396999 | 5.1:-66923284 | MS.gene81653:CDS |
| AGAGTTTGCCAGGTGGGAAT+TGG | 0.397987 | 5.1:-66919285 | MS.gene81653:CDS |
| AAAGCAATTTGAGGAACTTC+AGG | 0.401444 | 5.1:-66923674 | MS.gene81653:intron |
| CACTTCCCGAGTATACTCTT+TGG | 0.403272 | 5.1:-66923511 | MS.gene81653:CDS |
| CCTGCCTGTGGAACAGCATT+TGG | 0.404398 | 5.1:-66917980 | MS.gene81653:CDS |
| GTTGATGATATGGCTCTTCT+CGG | 0.410027 | 5.1:-66928938 | MS.gene81653:CDS |
| TCTTCTACTCTCTTCAAGTT+CGG | 0.410715 | 5.1:+66923990 | None:intergenic |
| TCAGCCTTATCTAAAGAAAT+GGG | 0.420197 | 5.1:-66922985 | MS.gene81653:CDS |
| GAAGCTTGAGGAAGAACTAA+TGG | 0.422228 | 5.1:-66918528 | MS.gene81653:CDS |
| AGCTGAAATAGAAGAGTTAA+GGG | 0.422995 | 5.1:-66920915 | MS.gene81653:CDS |
| ACACAGACCAGTATCTTGTT+TGG | 0.429741 | 5.1:+66923117 | None:intergenic |
| TGCTTCCTCCAGGCTGATGC+AGG | 0.438735 | 5.1:-66929283 | MS.gene81653:intron |
| CAGCCGCCTTAGAGTTTGCC+AGG | 0.439108 | 5.1:-66919295 | MS.gene81653:CDS |
| AGATACTGTTGATGATCATA+TGG | 0.440228 | 5.1:-66928574 | MS.gene81653:CDS |
| GCAGACAGAAGTCCAGAAAC+AGG | 0.440620 | 5.1:-66929227 | MS.gene81653:CDS |
| GCTCTTCTCGGGATACAAGC+TGG | 0.449889 | 5.1:-66928926 | MS.gene81653:CDS |
| CTCTTTAGCTTTAATAGCCT+CGG | 0.449984 | 5.1:+66920516 | None:intergenic |
| CAAATGTGCTGAACAGGATT+CGG | 0.450852 | 5.1:-66922240 | MS.gene81653:CDS |
| TCAAGATTTCTTTGTATGCA+TGG | 0.451174 | 5.1:+66918022 | None:intergenic |
| AATATTGACAAAGCAATTTG+AGG | 0.451416 | 5.1:-66923683 | MS.gene81653:CDS |
| ATCTAAAGAAATGGGGATGA+TGG | 0.454064 | 5.1:-66922977 | MS.gene81653:CDS |
| AATTACAAATAGTGAAGAAC+AGG | 0.462184 | 5.1:-66919473 | MS.gene81653:intron |
| GTTCAGCACATTTGTTTGCA+AGG | 0.462913 | 5.1:+66922249 | None:intergenic |
| CGTGAGACGCGGGAAGAAAC+GGG | 0.463036 | 5.1:+66930290 | None:intergenic |
| AATGCAAAAGGATGCAGCTA+TGG | 0.467843 | 5.1:-66923950 | MS.gene81653:CDS |
| TCCGTTCACTGCATCAGCAT+TGG | 0.477019 | 5.1:+66923915 | None:intergenic |
| CCTTTCATTTGCATCAAACT+GGG | 0.477201 | 5.1:+66920892 | None:intergenic |
| TCGTGAGACGCGGGAAGAAA+CGG | 0.486950 | 5.1:+66930289 | None:intergenic |
| CTACATAGAAGTAGATCATC+CGG | 0.493035 | 5.1:+66928711 | None:intergenic |
| TTATATCATGCAGGAGTTCA+CGG | 0.493537 | 5.1:-66918553 | MS.gene81653:intron |
| CGCCTTAGAGTTTGCCAGGT+GGG | 0.494648 | 5.1:-66919291 | MS.gene81653:CDS |
| TTCCACAGGCAGGACATTTG+CGG | 0.497983 | 5.1:+66917990 | None:intergenic |
| GAAGCCTGCAGAGAGAGCTA+TGG | 0.498135 | 5.1:-66923878 | MS.gene81653:CDS |
| ATGGAAAGAATTGAGGATAT+AGG | 0.501506 | 5.1:-66928555 | MS.gene81653:CDS |
| AGGCAGGACATTTGCGGTGA+CGG | 0.501630 | 5.1:+66917996 | None:intergenic |
| CGAGGCTATTAAAGCTAAAG+AGG | 0.502369 | 5.1:-66920515 | MS.gene81653:CDS |
| CTGTCCAAATGCTGTTCCAC+AGG | 0.503225 | 5.1:+66917976 | None:intergenic |
| AAGTACCTTCAGGATTATCA+AGG | 0.503978 | 5.1:+66928870 | None:intergenic |
| CTCTTCTCGGGATACAAGCT+GGG | 0.505460 | 5.1:-66928925 | MS.gene81653:CDS |
| CCTTTGCCAAAGAGTATACT+CGG | 0.508295 | 5.1:+66923505 | None:intergenic |
| GCAGGACTAACATTGCAAAA+CGG | 0.510569 | 5.1:-66923285 | MS.gene81653:CDS |
| AATCACTTCACGAAAATTGT+CGG | 0.513242 | 5.1:+66928319 | None:intergenic |
| CCCAGTTTGATGCAAATGAA+AGG | 0.514393 | 5.1:-66920892 | MS.gene81653:intron |
| CCAAATGCTGTTCCACAGGC+AGG | 0.515641 | 5.1:+66917980 | None:intergenic |
| GTTCACTGCATCAGCATTGG+AGG | 0.516207 | 5.1:+66923918 | None:intergenic |
| GCCTGGAGGATTATCAGAAA+AGG | 0.516683 | 5.1:-66921890 | MS.gene81653:intron |
| GAAGAGGCCAAACAAGATAC+TGG | 0.517357 | 5.1:-66923124 | MS.gene81653:intron |
| AAAAGGAATTGGATAGTGAA+AGG | 0.522583 | 5.1:-66919169 | MS.gene81653:intron |
| TTCTGAGCTTCAAGATTCCC+GGG | 0.523846 | 5.1:-66923716 | MS.gene81653:CDS |
| ATCATTGACAGAGTCTCTGC+AGG | 0.528509 | 5.1:-66923442 | MS.gene81653:intron |
| AGTCAGTCTGAAAATGCAAA+AGG | 0.529585 | 5.1:-66923962 | MS.gene81653:CDS |
| AAGAATTGAGGATATAGGTC+AGG | 0.532464 | 5.1:-66928550 | MS.gene81653:CDS |
| TGCAAAAGTGTATTAATGAA+AGG | 0.535858 | 5.1:-66923168 | MS.gene81653:CDS |
| TTTGCGTCGGTTATCTACTA+GGG | 0.536181 | 5.1:-66928616 | MS.gene81653:CDS |
| CATATGTATTATACCTTCCG+TGG | 0.537093 | 5.1:+66928509 | None:intergenic |
| AAGCCTGCAGAGAGAGCTAT+GGG | 0.539173 | 5.1:-66923877 | MS.gene81653:CDS |
| AATCAACCAACACTGGGATC+AGG | 0.540393 | 5.1:-66929131 | MS.gene81653:intron |
| TAATTGCAATCAACCAACAC+TGG | 0.541368 | 5.1:-66929138 | MS.gene81653:CDS |
| AAACCCATAGCTCTCTCTGC+AGG | 0.543797 | 5.1:+66923874 | None:intergenic |
| ATGCAAAAGGATGCAGCTAT+GGG | 0.546927 | 5.1:-66923949 | MS.gene81653:CDS |
| TATTAAAGCTAAAGAGGCTG+AGG | 0.549699 | 5.1:-66920509 | MS.gene81653:CDS |
| TCCTAGAGTCAGAAATGTCA+TGG | 0.550226 | 5.1:+66923212 | None:intergenic |
| GGAATTGGCAGATGCTGAGA+AGG | 0.550271 | 5.1:-66919270 | MS.gene81653:CDS |
| TAGATAACCGACGCAAAGCA+AGG | 0.550352 | 5.1:+66928622 | None:intergenic |
| ATCCAGAAAGATTCTGTGGA+GGG | 0.551161 | 5.1:-66928684 | MS.gene81653:CDS |
| GCTTGAATTTCGTGAGACGC+GGG | 0.552167 | 5.1:+66930280 | None:intergenic |
| CTCGGGATACAAGCTGGGAG+AGG | 0.556620 | 5.1:-66928920 | MS.gene81653:CDS |
| TTGATGATATGGCTCTTCTC+GGG | 0.557494 | 5.1:-66928937 | MS.gene81653:CDS |
| AGGAAGCCCTTGCTTTGCGT+CGG | 0.560789 | 5.1:-66928629 | MS.gene81653:CDS |
| CAAAGCTGAGTTTGGTGTGA+TGG | 0.562082 | 5.1:-66923010 | MS.gene81653:CDS |
| TGATCATATGGAAAGAATTG+AGG | 0.563216 | 5.1:-66928562 | MS.gene81653:CDS |
| TCCAATGCTGATGCAGTGAA+CGG | 0.564453 | 5.1:-66923916 | MS.gene81653:CDS |
| GATTACCTTGATAATCCTGA+AGG | 0.565357 | 5.1:-66928875 | MS.gene81653:intron |
| TAACATTGCAAAACGGGAGA+AGG | 0.566402 | 5.1:-66923278 | MS.gene81653:CDS |
| AGCATCTGCCAATTCCCACC+TGG | 0.566723 | 5.1:+66919277 | None:intergenic |
| TCCATGACATTTCTGACTCT+AGG | 0.566902 | 5.1:-66923213 | MS.gene81653:CDS |
| CACGAAGATTTATCCACGGA+AGG | 0.568556 | 5.1:-66928522 | MS.gene81653:intron |
| CGAGATTACTAATTATGTTG+AGG | 0.568617 | 5.1:-66928649 | MS.gene81653:CDS |
| CTGAAGCTGCTTGTGAACAA+AGG | 0.572664 | 5.1:-66920952 | MS.gene81653:CDS |
| GAGGGAGGACTGTAATATCA+AGG | 0.573477 | 5.1:-66920296 | MS.gene81653:intron |
| TTCAATTGAATTCTTCAACT+CGG | 0.577121 | 5.1:+66923849 | None:intergenic |
| CCAACGTCTATTACAGCAAG+TGG | 0.577654 | 5.1:-66920323 | MS.gene81653:CDS |
| CCGAGTATACTCTTTGGCAA+AGG | 0.579298 | 5.1:-66923505 | MS.gene81653:CDS |
| TCTTGAAATCAAGATGGAAG+AGG | 0.579606 | 5.1:-66923140 | MS.gene81653:CDS |
| ATCCGGAGGACACGAAGGAA+AGG | 0.581923 | 5.1:+66928728 | None:intergenic |
| ATTACAGCAAGTGGCTGAGA+GGG | 0.584569 | 5.1:-66920314 | MS.gene81653:CDS |
| AATAAAGTCGTCAAAAGCAA+TGG | 0.585648 | 5.1:-66922216 | MS.gene81653:intron |
| GAATGATCTTGAAATCAAGA+TGG | 0.585926 | 5.1:-66923146 | MS.gene81653:CDS |
| TGCTTGAATTTCGTGAGACG+CGG | 0.591340 | 5.1:+66930279 | None:intergenic |
| GAAACGGGAGTGAGAAGATG+AGG | 0.598522 | 5.1:+66930305 | None:intergenic |
| CGCTAAGAGAGAAATTGAGT+GGG | 0.598649 | 5.1:-66922888 | MS.gene81653:CDS |
| AACCTATATATCTGAAATCG+AGG | 0.599571 | 5.1:-66920482 | MS.gene81653:intron |
| GAATTCAATTGAAGAAGCGA+AGG | 0.600369 | 5.1:-66923839 | MS.gene81653:intron |
| TGGAAACACAGTTGAAGCGA+TGG | 0.602141 | 5.1:-66922957 | MS.gene81653:CDS |
| ATTAATCCAGAAAGATTCTG+TGG | 0.603677 | 5.1:-66928688 | MS.gene81653:CDS |
| CAATAACCTGATCCCAGTGT+TGG | 0.604541 | 5.1:+66929125 | None:intergenic |
| TGGAGTCTGCGGTTAATGCA+AGG | 0.604681 | 5.1:-66923240 | MS.gene81653:CDS |
| AGATAACCGACGCAAAGCAA+GGG | 0.606298 | 5.1:+66928623 | None:intergenic |
| CGAGCAGATTCAGAAAGATG+TGG | 0.606867 | 5.1:-66919201 | MS.gene81653:CDS |
| TCGCTAAGAGAGAAATTGAG+TGG | 0.608930 | 5.1:-66922889 | MS.gene81653:CDS |
| CACCGCAAATGTCCTGCCTG+TGG | 0.609399 | 5.1:-66917992 | MS.gene81653:CDS |
| AAACACAAGGAGTATACTGT+TGG | 0.609461 | 5.1:-66928281 | MS.gene81653:CDS |
| CTGTTCTGACCGTCCAAAGG+AGG | 0.610548 | 5.1:-66918399 | MS.gene81653:intron |
| GAATTCTAAATTGGAGTCTG+CGG | 0.618680 | 5.1:-66923251 | MS.gene81653:CDS |
| ACAGCAAGTGGCTGAGAGGG+AGG | 0.618824 | 5.1:-66920311 | MS.gene81653:CDS |
| CTTTGGACGGTCAGAACAGA+CGG | 0.621473 | 5.1:+66918403 | None:intergenic |
| AAGAGAGAAATTGAGTGGGA+AGG | 0.622883 | 5.1:-66922884 | MS.gene81653:intron |
| AGGAGAGTTTGACGAAATCG+TGG | 0.625770 | 5.1:-66924013 | MS.gene81653:CDS |
| CGTCTGTTCTGACCGTCCAA+AGG | 0.628492 | 5.1:-66918402 | MS.gene81653:CDS |
| AATTGCAATCAACCAACACT+GGG | 0.631357 | 5.1:-66929137 | MS.gene81653:CDS |
| GAGTTCACGGAAGAAGCTTG+AGG | 0.633563 | 5.1:-66918540 | MS.gene81653:CDS |
| GTGAAGGCAAAACATTTACA+TGG | 0.635152 | 5.1:-66919574 | MS.gene81653:CDS |
| CAGAAAGATTCTGTGGAGGG+AGG | 0.638260 | 5.1:-66928681 | MS.gene81653:CDS |
| TTTGGACATGTATGGCCTGG+AGG | 0.642076 | 5.1:-66921904 | MS.gene81653:CDS |
| CAGCCTTATCTAAAGAAATG+GGG | 0.644025 | 5.1:-66922984 | MS.gene81653:CDS |
| AATCCAGAAAGATTCTGTGG+AGG | 0.648780 | 5.1:-66928685 | MS.gene81653:CDS |
| ACAGATTGAAAAGTTACAGG+AGG | 0.653733 | 5.1:-66921949 | MS.gene81653:intron |
| TATTACAGCAAGTGGCTGAG+AGG | 0.658845 | 5.1:-66920315 | MS.gene81653:CDS |
| CCACTTGCTGTAATAGACGT+TGG | 0.660621 | 5.1:+66920323 | None:intergenic |
| AGATCATCCGGAGGACACGA+AGG | 0.660758 | 5.1:+66928723 | None:intergenic |
| TACTCTCCATGCAAAACACA+AGG | 0.662712 | 5.1:-66928294 | MS.gene81653:CDS |
| GCTTGTATCAGAAAGCGTGA+AGG | 0.670954 | 5.1:-66919590 | MS.gene81653:CDS |
| CTTACACGAAGATTTATCCA+CGG | 0.672505 | 5.1:-66928526 | MS.gene81653:CDS |
| CTTTGCCAAAGAGTATACTC+GGG | 0.675113 | 5.1:+66923506 | None:intergenic |
| CAAACTCTAAGGCGGCTGCG+AGG | 0.683577 | 5.1:+66919300 | None:intergenic |
| CCGCCTTAGAGTTTGCCAGG+TGG | 0.688256 | 5.1:-66919292 | MS.gene81653:CDS |
| CCACCTGGCAAACTCTAAGG+CGG | 0.716860 | 5.1:+66919292 | None:intergenic |
| CATAGAAGTAGATCATCCGG+AGG | 0.734057 | 5.1:+66928714 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 66917949 | 66930366 | 66917949 | ID=MS.gene81653 |
| chr5.1 | mRNA | 66917949 | 66930366 | 66917949 | ID=MS.gene81653.t1;Parent=MS.gene81653 |
| chr5.1 | exon | 66930256 | 66930366 | 66930256 | ID=MS.gene81653.t1.exon1;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66930256 | 66930366 | 66930256 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66929132 | 66929293 | 66929132 | ID=MS.gene81653.t1.exon2;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66929132 | 66929293 | 66929132 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66928876 | 66928963 | 66928876 | ID=MS.gene81653.t1.exon3;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66928876 | 66928963 | 66928876 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66928523 | 66928753 | 66928523 | ID=MS.gene81653.t1.exon4;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66928523 | 66928753 | 66928523 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66928222 | 66928389 | 66928222 | ID=MS.gene81653.t1.exon5;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66928222 | 66928389 | 66928222 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66923840 | 66924033 | 66923840 | ID=MS.gene81653.t1.exon6;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66923840 | 66924033 | 66923840 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66923675 | 66923758 | 66923675 | ID=MS.gene81653.t1.exon7;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66923675 | 66923758 | 66923675 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66923443 | 66923562 | 66923443 | ID=MS.gene81653.t1.exon8;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66923443 | 66923562 | 66923443 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66923125 | 66923311 | 66923125 | ID=MS.gene81653.t1.exon9;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66923125 | 66923311 | 66923125 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66922885 | 66923045 | 66922885 | ID=MS.gene81653.t1.exon10;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66922885 | 66923045 | 66922885 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66922217 | 66922288 | 66922217 | ID=MS.gene81653.t1.exon11;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66922217 | 66922288 | 66922217 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66921891 | 66921967 | 66921891 | ID=MS.gene81653.t1.exon12;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66921891 | 66921967 | 66921891 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66920893 | 66921084 | 66920893 | ID=MS.gene81653.t1.exon13;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66920893 | 66921084 | 66920893 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66920483 | 66920555 | 66920483 | ID=MS.gene81653.t1.exon14;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66920483 | 66920555 | 66920483 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66920297 | 66920383 | 66920297 | ID=MS.gene81653.t1.exon15;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66920297 | 66920383 | 66920297 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66919474 | 66919611 | 66919474 | ID=MS.gene81653.t1.exon16;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66919474 | 66919611 | 66919474 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66919170 | 66919372 | 66919170 | ID=MS.gene81653.t1.exon17;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66919170 | 66919372 | 66919170 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66918400 | 66918562 | 66918400 | ID=MS.gene81653.t1.exon18;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66918400 | 66918562 | 66918400 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| chr5.1 | exon | 66917949 | 66918080 | 66917949 | ID=MS.gene81653.t1.exon19;Parent=MS.gene81653.t1 |
| chr5.1 | CDS | 66917949 | 66918080 | 66917949 | ID=cds.MS.gene81653.t1;Parent=MS.gene81653.t1 |
| Gene Sequence |
| Protein sequence |
