AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88667


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88667.t1 MTR_0693s0030 96.241 133 5 0 1 133 1 133 5.02e-91 261
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88667.t1 AT4G25830 33.333 129 77 2 4 132 3 122 1.06e-17 75.1
MS.gene88667.t1 AT4G16442 33.333 135 81 3 1 133 4 131 3.94e-12 60.8

Find 17 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTGAAATTCCGTATTGT+AGG 0.365530 8.1:-4438289 None:intergenic
TATGTGACATCTGCAGCTGC+TGG 0.381099 8.1:+4438223 MS.gene88667:CDS
CCTACAATACGGAATTTCAA+TGG 0.448706 8.1:+4438289 MS.gene88667:CDS
CTTATTATATGCACATGGCT+TGG 0.454545 8.1:+4438314 MS.gene88667:CDS
GATTAGTTTCTTATTAGATC+AGG 0.461935 8.1:+4438336 MS.gene88667:CDS
TCTCAGCTTGTCCTACAATA+CGG 0.494365 8.1:+4438278 MS.gene88667:CDS
AAACTTCTCCTCTAGCCATT+CGG 0.500585 8.1:-4437968 None:intergenic
TGTTGCATACCTTCATCCAC+TGG 0.513091 8.1:-4438912 None:intergenic
GAAAGCTACTTACAAGGACT+TGG 0.522093 8.1:+4438085 MS.gene88667:CDS
TTCAAATGCTGAAGTGTTTG+TGG 0.537098 8.1:-4438854 None:intergenic
ATGTGACATCTGCAGCTGCT+GGG 0.542822 8.1:+4438224 MS.gene88667:CDS
AGAAGCATTCCAGTGGATGA+AGG 0.566952 8.1:+4438903 MS.gene88667:CDS
TAAGAAGAAAGCTACTTACA+AGG 0.592857 8.1:+4438079 MS.gene88667:CDS
TGGTTCTTATTATATGCACA+TGG 0.599744 8.1:+4438309 MS.gene88667:CDS
TGAAGAAATGGAATTCCGAA+TGG 0.602583 8.1:+4437953 None:intergenic
ATGGAATTCCGAATGGCTAG+AGG 0.629083 8.1:+4437960 MS.gene88667:CDS
CTGGATCAGAAGCATTCCAG+TGG 0.661575 8.1:+4438896 MS.gene88667:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAAAATTGAATGAC+AGG + chr8.1:4438567-4438586 MS.gene88667:intron 15.0%
!! AAAATTAAAATTGAATGACA+GGG + chr8.1:4438568-4438587 MS.gene88667:intron 15.0%
!! TACTTATACATGATAATATA+AGG + chr8.1:4438497-4438516 MS.gene88667:intron 15.0%
!!! AAAAAGATAATCATTTTGTT+TGG + chr8.1:4438604-4438623 MS.gene88667:intron 15.0%
!!! GAATTTTTGTATGTTTTTTT+AGG + chr8.1:4438191-4438210 MS.gene88667:intron 15.0%
! AAATTCCTAATTCAAGATCA+TGG - chr8.1:4438177-4438196 None:intergenic 25.0%
! AATAATCATTTGGCAAATCT+TGG + chr8.1:4438796-4438815 MS.gene88667:intron 25.0%
! AGAGAACAAACAAATAAAGA+GGG - chr8.1:4438131-4438150 None:intergenic 25.0%
! ATATACCATGATCTTGAATT+AGG + chr8.1:4438169-4438188 MS.gene88667:intron 25.0%
! CCAAATGATTATTTCAATCA+AGG - chr8.1:4438789-4438808 None:intergenic 25.0%
! CCTTGATTGAAATAATCATT+TGG + chr8.1:4438786-4438805 MS.gene88667:intron 25.0%
! TACTCGAATCAAAATAAACT+TGG + chr8.1:4438471-4438490 MS.gene88667:intron 25.0%
! TCATTTCCTCTTCAAAAATT+AGG + chr8.1:4438524-4438543 MS.gene88667:intron 25.0%
! TGATATCATAGCCATTAAAA+TGG - chr8.1:4438995-4439014 None:intergenic 25.0%
!! ATTATCTTTTTCTTCCTACT+GGG - chr8.1:4438595-4438614 None:intergenic 25.0%
!! CTATATAGCATCCATTTTAA+TGG + chr8.1:4438981-4439000 MS.gene88667:intron 25.0%
!!! GATTAGTTTCTTATTAGATC+AGG + chr8.1:4438336-4438355 MS.gene88667:CDS 25.0%
AAGTAAGCTCAAATCCAATT+AGG + chr8.1:4438448-4438467 MS.gene88667:intron 30.0%
GAGAGAACAAACAAATAAAG+AGG - chr8.1:4438132-4438151 None:intergenic 30.0%
GCATAACTCTTAAAGTAGTT+AGG + chr8.1:4438696-4438715 MS.gene88667:intron 30.0%
TAAGAAGAAAGCTACTTACA+AGG + chr8.1:4438079-4438098 MS.gene88667:CDS 30.0%
TGGTTCTTATTATATGCACA+TGG + chr8.1:4438309-4438328 MS.gene88667:CDS 30.0%
! GATTATCTTTTTCTTCCTAC+TGG - chr8.1:4438596-4438615 None:intergenic 30.0%
! TGCTTTTGATACTGAAACAA+AGG + chr8.1:4438040-4438059 MS.gene88667:CDS 30.0%
!! GAGATACCTAATTTTTGAAG+AGG - chr8.1:4438533-4438552 None:intergenic 30.0%
!! TTTTGATTCGAGTACCTAAT+TGG - chr8.1:4438465-4438484 None:intergenic 30.0%
ATAATAATGCCCCTCCAATT+TGG - chr8.1:4438957-4438976 None:intergenic 35.0%
CCATTGAAATTCCGTATTGT+AGG - chr8.1:4438292-4438311 None:intergenic 35.0%
CCTACAATACGGAATTTCAA+TGG + chr8.1:4438289-4438308 MS.gene88667:CDS 35.0%
CTTATTATATGCACATGGCT+TGG + chr8.1:4438314-4438333 MS.gene88667:CDS 35.0%
! TTCAAATGCTGAAGTGTTTG+TGG - chr8.1:4438857-4438876 None:intergenic 35.0%
AAACTTCTCCTCTAGCCATT+CGG - chr8.1:4437971-4437990 None:intergenic 40.0%
ACCAGATTCTGCTACCAAAT+TGG + chr8.1:4438940-4438959 MS.gene88667:intron 40.0%
AGATTCTGCTACCAAATTGG+AGG + chr8.1:4438943-4438962 MS.gene88667:intron 40.0%
ATTCTGCTACCAAATTGGAG+GGG + chr8.1:4438945-4438964 MS.gene88667:intron 40.0%
GAAAGCTACTTACAAGGACT+TGG + chr8.1:4438085-4438104 MS.gene88667:CDS 40.0%
GATTCTGCTACCAAATTGGA+GGG + chr8.1:4438944-4438963 MS.gene88667:intron 40.0%
TCCAGTAAGTGCAAAAAGAC+AGG - chr8.1:4438881-4438900 None:intergenic 40.0%
TCTCAGCTTGTCCTACAATA+CGG + chr8.1:4438278-4438297 MS.gene88667:CDS 40.0%
! TCCAATTTGGTAGCAGAATC+TGG - chr8.1:4438944-4438963 None:intergenic 40.0%
ATGGAATTCCGAATGGCTAG+AGG + chr8.1:4437960-4437979 MS.gene88667:CDS 45.0%
TGTTGCATACCTTCATCCAC+TGG - chr8.1:4438915-4438934 None:intergenic 45.0%
! GCCTGTCTTTTTGCACTTAC+TGG + chr8.1:4438877-4438896 MS.gene88667:CDS 45.0%
!! AGAAGCATTCCAGTGGATGA+AGG + chr8.1:4438903-4438922 MS.gene88667:CDS 45.0%
!! ATATAATTATATACAATTAT+AGG + chr8.1:4438400-4438419 MS.gene88667:intron 5.0%
ATGTGACATCTGCAGCTGCT+GGG + chr8.1:4438224-4438243 MS.gene88667:CDS 50.0%
TATGTGACATCTGCAGCTGC+TGG + chr8.1:4438223-4438242 MS.gene88667:CDS 50.0%
TTGAATGACAGGGACCCAGT+AGG + chr8.1:4438578-4438597 MS.gene88667:intron 50.0%
!! CTGGATCAGAAGCATTCCAG+TGG + chr8.1:4438896-4438915 MS.gene88667:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 4437960 4439039 4437960 ID=MS.gene88667
chr8.1 mRNA 4437960 4439039 4437960 ID=MS.gene88667.t1;Parent=MS.gene88667
chr8.1 exon 4437960 4438117 4437960 ID=MS.gene88667.t1.exon1;Parent=MS.gene88667.t1
chr8.1 CDS 4437960 4438117 4437960 ID=cds.MS.gene88667.t1;Parent=MS.gene88667.t1
chr8.1 exon 4438213 4438357 4438213 ID=MS.gene88667.t1.exon2;Parent=MS.gene88667.t1
chr8.1 CDS 4438213 4438357 4438213 ID=cds.MS.gene88667.t1;Parent=MS.gene88667.t1
chr8.1 exon 4438832 4438924 4438832 ID=MS.gene88667.t1.exon3;Parent=MS.gene88667.t1
chr8.1 CDS 4438832 4438924 4438832 ID=cds.MS.gene88667.t1;Parent=MS.gene88667.t1
chr8.1 exon 4439025 4439039 4439025 ID=MS.gene88667.t1.exon4;Parent=MS.gene88667.t1
chr8.1 CDS 4439025 4439039 4439025 ID=cds.MS.gene88667.t1;Parent=MS.gene88667.t1
Gene Sequence

>MS.gene88667

ATGGAATTCCGAATGGCTAGAGGAGAAGTTTTGCTAAGAGTTTCAGCAATATTGTTCTTAGTATCAACAGCTTGTATAGTTGCTTTTGATACTGAAACAAAGGTTGTAATATTAACGATTAAGAAGAAAGCTACTTACAAGGACTTGGATGCTCTCAAGTAAAGTTCTCCCTCTTTATTTGTTTGTTCTCTCACATTTTATGTTGAAATATATACCATGATCTTGAATTAGGAATTTTTGTATGTTTTTTTAGGATCTTGTTGTATGTGACATCTGCAGCTGCTGGGTATAACATGCTTCAATTATGCAAACACTCTTTCTCAGCTTGTCCTACAATACGGAATTTCAATGGTTCTTATTATATGCACATGGCTTGGATTAGTTTCTTATTAGATCAGGTAAATAAACTCTTTACTTATTTAGCTGAGAATACATATTACATATAATTATATACAATTATAGGTTGATCAAAGTATGCATATTAATTGAAGTAAGCTCAAATCCAATTAGGTACTCGAATCAAAATAAACTTGGTTATACTTATACATGATAATATAAGGTATCTCATTTCCTCTTCAAAAATTAGGTATCTCATATTCTTGACATAAAAAATTAAAATTGAATGACAGGGACCCAGTAGGAAGAAAAAGATAATCATTTTGTTTGGAGAGCTTAATTTTGATGCATTGTCAATATTAAAAGTTTTACGCTCTCAACAAATCACAATCAATCATATGCATAACTCTTAAAGTAGTTAGGATTAAAGTCAATATTTGTAATGATCTTTTGATTAAGATTGATACTTAACAGTGCATATGAATTAAATCCTTGATTGAAATAATCATTTGGCAAATCTTGGTTTTTAATAACAGATTGTTGTGTACTTGACATTCGCCACAAACACTTCAGCATTTGAAGCCTGTCTTTTTGCACTTACTGGATCAGAAGCATTCCAGTGGATGAAGGTATGCAACAAATTCACCAGATTCTGCTACCAAATTGGAGGGGCATTATTATGCTGCTATATAGCATCCATTTTAATGGCTATGATATCAACTATCTCAGCTTACAAAGTTTTAA

Protein sequence

>MS.gene88667.t1

MEFRMARGEVLLRVSAILFLVSTACIVAFDTETKVVILTIKKKATYKDLDALKILLYVTSAAAGYNMLQLCKHSFSACPTIRNFNGSYYMHMAWISFLLDQIVVYLTFATNTSAFEACLFALTGSEAFQWMKLTKF