AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90704


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90704.t1 MTR_1g022450 98.507 134 2 0 1 134 1 134 1.81e-96 273
MS.gene90704.t1 MTR_1g022450 97.059 136 2 1 1 134 1 136 1.30e-94 269
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90704.t1 AT1G75350 59.398 133 42 2 1 121 1 133 1.76e-52 162

Find 33 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAACCACATACTCTTTC+TGG 0.120517 1.4:+1797878 None:intergenic
TTAGCATCTTCGTAGAATTT+TGG 0.129378 1.4:+1797943 None:intergenic
ATGGAGATTCCTGTGCTTAA+AGG 0.247138 1.4:-1797748 MS.gene90704:CDS
CGGTGAATTGTCGCAGATTA+TGG 0.250630 1.4:-1797767 MS.gene90704:CDS
GGAAAGAAAGTGGGCAATTT+CGG 0.276920 1.4:-1797994 MS.gene90704:CDS
AGGTAACAGGTCTGCTCTTA+TGG 0.321423 1.4:-1797827 MS.gene90704:CDS
GACCTGTTACCTAGGTAAAA+TGG 0.333258 1.4:+1797838 None:intergenic
TTAATCCCTTTACGTCTAGA+AGG 0.369196 1.4:+1797712 None:intergenic
TTATTCCTTCTAGACGTAAA+GGG 0.415844 1.4:-1797717 MS.gene90704:CDS
ATCAACCACATACTCTTTCT+GGG 0.433365 1.4:+1797879 None:intergenic
ATAACCAATACCTTTGTTCA+AGG 0.436471 1.4:-1798382 MS.gene90704:CDS
GTTATTCCTTCTAGACGTAA+AGG 0.445368 1.4:-1797718 MS.gene90704:CDS
TATGTGGTTGATGTTTGGTC+TGG 0.461863 1.4:-1797868 MS.gene90704:CDS
TCTTATGGTGAGTGATGATC+AGG 0.479241 1.4:-1797812 MS.gene90704:CDS
AAGAGTATGTGGTTGATGTT+TGG 0.479372 1.4:-1797873 MS.gene90704:CDS
GAGAAGTTTCGTAAGAAGTT+CGG 0.493517 1.4:-1797787 MS.gene90704:CDS
AACGAGCTTGTGATGACAAC+TGG 0.499125 1.4:-1797907 MS.gene90704:CDS
ATGTGGTTGATGTTTGGTCT+GGG 0.499973 1.4:-1797867 MS.gene90704:CDS
TTTGTGGGAAGTGGAAGGAA+TGG 0.505762 1.4:+1798355 None:intergenic
TAAGAGCAGACCTGTTACCT+AGG 0.535799 1.4:+1797830 None:intergenic
AAAGGGATTAAAAGTGGTGG+AGG 0.538533 1.4:-1797700 MS.gene90704:CDS
AAAATTCTACGAAGATGCTA+AGG 0.547901 1.4:-1797941 MS.gene90704:CDS
ACAATCTCACCTTTAAGCAC+AGG 0.575557 1.4:+1797739 None:intergenic
GAATCAACAAGGAAAGAAAG+TGG 0.577082 1.4:-1798004 MS.gene90704:CDS
AGACGTAAAGGGATTAAAAG+TGG 0.579688 1.4:-1797706 MS.gene90704:CDS
CGTAAAGGGATTAAAAGTGG+TGG 0.606986 1.4:-1797703 MS.gene90704:CDS
GTTTGGTTGCAGAATCAACA+AGG 0.616245 1.4:-1798015 MS.gene90704:intron
AATCAACAAGGAAAGAAAGT+GGG 0.633997 1.4:-1798003 MS.gene90704:CDS
AAGGGATTAAAAGTGGTGGA+GGG 0.644540 1.4:-1797699 MS.gene90704:CDS
TGTTACCTAGGTAAAATGGA+TGG 0.664203 1.4:+1797842 None:intergenic
TGGAACCCAGAAAGAGTATG+TGG 0.674951 1.4:-1797884 MS.gene90704:CDS
GAGCTTGTGATGACAACTGG+TGG 0.675964 1.4:-1797904 MS.gene90704:CDS
TATGGTGAGTGATGATCAGG+TGG 0.684535 1.4:-1797809 MS.gene90704:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTAATTTCCTAAAT+AGG - chr1.4:1797868-1797887 MS.gene90704:CDS 10.0%
!! ATATTCTAATAGAAATGATA+AGG + chr1.4:1797778-1797797 None:intergenic 15.0%
!! GAAACAATAATATGAATAAT+TGG + chr1.4:1797994-1798013 None:intergenic 15.0%
!! AACAAAACAAAAAAACATGT+TGG + chr1.4:1798030-1798049 None:intergenic 20.0%
!! AATATGTCTATATTAGAATC+AGG + chr1.4:1797961-1797980 None:intergenic 20.0%
!! ATATGTCTATATTAGAATCA+GGG + chr1.4:1797960-1797979 None:intergenic 20.0%
! AACTGTTTACAAACCTATTT+AGG + chr1.4:1797884-1797903 None:intergenic 25.0%
! CAAATGATATCAATACAATC+AGG - chr1.4:1797917-1797936 MS.gene90704:CDS 25.0%
! TAATAGAAATGATAAGGAAG+TGG + chr1.4:1797772-1797791 None:intergenic 25.0%
AAAATTCTACGAAGATGCTA+AGG - chr1.4:1798140-1798159 MS.gene90704:intron 30.0%
AATCAACAAGGAAAGAAAGT+GGG - chr1.4:1798078-1798097 MS.gene90704:intron 30.0%
ATAACCAATACCTTTGTTCA+AGG - chr1.4:1797699-1797718 MS.gene90704:CDS 30.0%
TTATTCCTTCTAGACGTAAA+GGG - chr1.4:1798364-1798383 MS.gene90704:CDS 30.0%
TTCTCATGAATTGTGATTCA+TGG + chr1.4:1797849-1797868 None:intergenic 30.0%
! TAGCATCTTCGTAGAATTTT+GGG + chr1.4:1798140-1798159 None:intergenic 30.0%
! TTAGCATCTTCGTAGAATTT+TGG + chr1.4:1798141-1798160 None:intergenic 30.0%
! TTTTTGTGTGTGTTTGTGTT+TGG - chr1.4:1798049-1798068 MS.gene90704:intron 30.0%
!! TTTTCCTTGAACAAAGGTAT+TGG + chr1.4:1797706-1797725 None:intergenic 30.0%
!!! TACTACAGTACGTTTTTTGT+GGG + chr1.4:1797744-1797763 None:intergenic 30.0%
!!! TTACTACAGTACGTTTTTTG+TGG + chr1.4:1797745-1797764 None:intergenic 30.0%
AGAAATGATAAGGAAGTGGT+TGG + chr1.4:1797768-1797787 None:intergenic 35.0%
AGACGTAAAGGGATTAAAAG+TGG - chr1.4:1798375-1798394 MS.gene90704:CDS 35.0%
ATCAACCACATACTCTTTCT+GGG + chr1.4:1798205-1798224 None:intergenic 35.0%
GAATCAACAAGGAAAGAAAG+TGG - chr1.4:1798077-1798096 MS.gene90704:intron 35.0%
GAGAAGTTTCGTAAGAAGTT+CGG - chr1.4:1798294-1798313 MS.gene90704:intron 35.0%
GTTATTCCTTCTAGACGTAA+AGG - chr1.4:1798363-1798382 MS.gene90704:CDS 35.0%
TGTGATTCATGGCTAATGAA+TGG + chr1.4:1797838-1797857 None:intergenic 35.0%
TGTTACCTAGGTAAAATGGA+TGG + chr1.4:1798242-1798261 None:intergenic 35.0%
TTAATCCCTTTACGTCTAGA+AGG + chr1.4:1798372-1798391 None:intergenic 35.0%
! AAGAGTATGTGGTTGATGTT+TGG - chr1.4:1798208-1798227 MS.gene90704:intron 35.0%
! ATCCATTTTACCTAGGTAAC+AGG - chr1.4:1798241-1798260 MS.gene90704:intron 35.0%
! GAATGGTTTTCCTTGAACAA+AGG + chr1.4:1797712-1797731 None:intergenic 35.0%
AAAGGGATTAAAAGTGGTGG+AGG - chr1.4:1798381-1798400 MS.gene90704:CDS 40.0%
ACAATCTCACCTTTAAGCAC+AGG + chr1.4:1798345-1798364 None:intergenic 40.0%
ATGGAGATTCCTGTGCTTAA+AGG - chr1.4:1798333-1798352 MS.gene90704:intron 40.0%
CATCAACCACATACTCTTTC+TGG + chr1.4:1798206-1798225 None:intergenic 40.0%
CGTAAAGGGATTAAAAGTGG+TGG - chr1.4:1798378-1798397 MS.gene90704:CDS 40.0%
GACCTGTTACCTAGGTAAAA+TGG + chr1.4:1798246-1798265 None:intergenic 40.0%
GGAAAGAAAGTGGGCAATTT+CGG - chr1.4:1798087-1798106 MS.gene90704:intron 40.0%
GTTTGGTTGCAGAATCAACA+AGG - chr1.4:1798066-1798085 MS.gene90704:intron 40.0%
TCTTATGGTGAGTGATGATC+AGG - chr1.4:1798269-1798288 MS.gene90704:intron 40.0%
! AAGGGATTAAAAGTGGTGGA+GGG - chr1.4:1798382-1798401 MS.gene90704:CDS 40.0%
! ATGTGGTTGATGTTTGGTCT+GGG - chr1.4:1798214-1798233 MS.gene90704:intron 40.0%
! TATGTGGTTGATGTTTGGTC+TGG - chr1.4:1798213-1798232 MS.gene90704:intron 40.0%
!!! AGTACGTTTTTTGTGGGAAG+TGG + chr1.4:1797738-1797757 None:intergenic 40.0%
AACGAGCTTGTGATGACAAC+TGG - chr1.4:1798174-1798193 MS.gene90704:intron 45.0%
AGGTAACAGGTCTGCTCTTA+TGG - chr1.4:1798254-1798273 MS.gene90704:intron 45.0%
CGGTGAATTGTCGCAGATTA+TGG - chr1.4:1798314-1798333 MS.gene90704:intron 45.0%
TAAGAGCAGACCTGTTACCT+AGG + chr1.4:1798254-1798273 None:intergenic 45.0%
TATGGTGAGTGATGATCAGG+TGG - chr1.4:1798272-1798291 MS.gene90704:intron 45.0%
TTTGTGGGAAGTGGAAGGAA+TGG + chr1.4:1797729-1797748 None:intergenic 45.0%
! GGGAACCATCCATTTTACCT+AGG - chr1.4:1798234-1798253 MS.gene90704:intron 45.0%
! TGGAACCCAGAAAGAGTATG+TGG - chr1.4:1798197-1798216 MS.gene90704:intron 45.0%
!!! CGTTTTTTGTGGGAAGTGGA+AGG + chr1.4:1797734-1797753 None:intergenic 45.0%
GAGCTTGTGATGACAACTGG+TGG - chr1.4:1798177-1798196 MS.gene90704:intron 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 1797687 1798416 1797687 ID=MS.gene90704
chr1.4 mRNA 1797687 1798416 1797687 ID=MS.gene90704.t1;Parent=MS.gene90704
chr1.4 exon 1798351 1798416 1798351 ID=MS.gene90704.t1.exon1;Parent=MS.gene90704.t1
chr1.4 CDS 1798351 1798416 1798351 ID=cds.MS.gene90704.t1;Parent=MS.gene90704.t1
chr1.4 exon 1797687 1798025 1797687 ID=MS.gene90704.t1.exon2;Parent=MS.gene90704.t1
chr1.4 CDS 1797687 1798025 1797687 ID=cds.MS.gene90704.t1;Parent=MS.gene90704.t1
Gene Sequence

>MS.gene90704

ATGGCGCAGTGCATAACCAATACCTTTGTTCAAGGAAAACCATTCCTTCCACTTCCCACAAAAAACGTACTGTAGTAACCAACCACTTCCTTATCATTTCTATTAGAATATGTCTATATTCATTGTTTCACTTGTAAATATTGATTAGCCATTCATTAGCCATGAATCACAATTCATGAGAATTTATTTAATTTCCTAAATAGGTTTGTAAACAGTTGTATATATTTTCACAAATGATATCAATACAATCAGGAAATTATACTCTTGATTCCCTGATTCTAATATAGACATATTCTAATAATTTCCAATTATTCATATTATTGTTTCTGTATTAAACTAACCAACATGTTTTTTTGTTTTGTTTTTTGTGTGTGTTTGTGTTTGGTTGCAGAATCAACAAGGAAAGAAAGTGGGCAATTTCGGAGTGTATTGCAAGAAGAAAGACATACACCCAAAATTCTACGAAGATGCTAAGGTTTACTGTAACAACGAGCTTGTGATGACAACTGGTGGAACCCAGAAAGAGTATGTGGTTGATGTTTGGTCTGGGAACCATCCATTTTACCTAGGTAACAGGTCTGCTCTTATGGTGAGTGATGATCAGGTGGAGAAGTTTCGTAAGAAGTTCGGTGAATTGTCGCAGATTATGGAGATTCCTGTGCTTAAAGGTGAGATTGTTATTCCTTCTAGACGTAAAGGGATTAAAAGTGGTGGAGGGAAGAAGAAGTGA

Protein sequence

>MS.gene90704.t1

MAQCITNTFVQGKPFLPLPTKNNQQGKKVGNFGVYCKKKDIHPKFYEDAKVYCNNELVMTTGGTQKEYVVDVWSGNHPFYLGNRSALMVSDDQVEKFRKKFGELSQIMEIPVLKGEIVIPSRRKGIKSGGGKKK