Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95596.t1 | XP_013449138.1 | 86.1 | 280 | 39 | 0 | 1 | 280 | 1 | 280 | 1.10E-141 | 512.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95596.t1 | Q9SRM5 | 59.0 | 251 | 101 | 2 | 31 | 279 | 5 | 255 | 5.9e-88 | 325.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95596.t1 | A0A072UBE2 | 86.1 | 280 | 39 | 0 | 1 | 280 | 1 | 280 | 7.8e-142 | 512.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene95596 | -0.831802 | 1.36E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene95596 | -0.806061 | 9.88E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene95596 | -0.805677 | 1.19E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene95596 | -0.821803 | 3.32E-53 | -1.69E-46 |
| MS.gene04932 | MS.gene95596 | 0.828389 | 9.24E-55 | -1.69E-46 |
| MS.gene049549 | MS.gene95596 | 0.812939 | 3.29E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene95596 | 0.828111 | 1.08E-54 | -1.69E-46 |
| MS.gene049633 | MS.gene95596 | -0.808738 | 2.67E-50 | -1.69E-46 |
| MS.gene049634 | MS.gene95596 | 0.811588 | 6.49E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene95596 | -0.82572 | 4.02E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene95596 | -0.808268 | 3.37E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene95596 | 0.801317 | 9.53E-49 | -1.69E-46 |
| MS.gene050444 | MS.gene95596 | -0.800947 | 1.13E-48 | -1.69E-46 |
| MS.gene050496 | MS.gene95596 | -0.816991 | 4.16E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene95596 | -0.82728 | 1.71E-54 | -1.69E-46 |
| MS.gene050518 | MS.gene95596 | -0.801058 | 1.08E-48 | -1.69E-46 |
| MS.gene050538 | MS.gene95596 | -0.809762 | 1.61E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene95596 | -0.8243 | 8.70E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene95596 | -0.829805 | 4.19E-55 | -1.69E-46 |
| MS.gene050675 | MS.gene95596 | -0.817195 | 3.74E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene95596 | -0.818374 | 2.03E-52 | -1.69E-46 |
| MS.gene05079 | MS.gene95596 | 0.805745 | 1.15E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene95596 | 0.80437 | 2.23E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene95596 | -0.806659 | 7.39E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene95596 | -0.823436 | 1.39E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene95596 | -0.822113 | 2.82E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene95596 | -0.804356 | 2.25E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene95596 | 0.825054 | 5.78E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene95596 | 0.808377 | 3.19E-50 | -1.69E-46 |
| MS.gene051131 | MS.gene95596 | 0.800948 | 1.13E-48 | -1.69E-46 |
| MS.gene051138 | MS.gene95596 | 0.819344 | 1.22E-52 | -1.69E-46 |
| MS.gene051317 | MS.gene95596 | -0.802953 | 4.39E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene95596 | 0.80046 | 1.43E-48 | -1.69E-46 |
| MS.gene051366 | MS.gene95596 | 0.803775 | 2.97E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene95596 | 0.838868 | 2.23E-57 | -1.69E-46 |
| MS.gene051749 | MS.gene95596 | 0.805397 | 1.36E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene95596 | -0.810897 | 9.17E-51 | -1.69E-46 |
| MS.gene052129 | MS.gene95596 | 0.815385 | 9.50E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene95596 | -0.800712 | 1.27E-48 | -1.69E-46 |
| MS.gene05240 | MS.gene95596 | 0.828305 | 9.68E-55 | -1.69E-46 |
| MS.gene052606 | MS.gene95596 | 0.81299 | 3.21E-51 | -1.69E-46 |
| MS.gene052702 | MS.gene95596 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene95596 | -0.812809 | 3.52E-51 | -1.69E-46 |
| MS.gene053033 | MS.gene95596 | 0.803167 | 3.97E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene95596 | -0.804247 | 2.37E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene95596 | 0.833306 | 5.75E-56 | -1.69E-46 |
| MS.gene053479 | MS.gene95596 | -0.835892 | 1.29E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene95596 | -0.815969 | 7.04E-52 | -1.69E-46 |
| MS.gene054181 | MS.gene95596 | 0.823389 | 1.42E-53 | -1.69E-46 |
| MS.gene054290 | MS.gene95596 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene054642 | MS.gene95596 | -0.806859 | 6.70E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene95596 | 0.818582 | 1.82E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene95596 | -0.817591 | 3.05E-52 | -1.69E-46 |
| MS.gene05483 | MS.gene95596 | 0.80036 | 1.50E-48 | -1.69E-46 |
| MS.gene054884 | MS.gene95596 | 0.814522 | 1.48E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene95596 | -0.806874 | 6.66E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene95596 | 0.818904 | 1.54E-52 | -1.69E-46 |
| MS.gene055158 | MS.gene95596 | 0.814179 | 1.76E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene95596 | -0.813266 | 2.79E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene95596 | -0.824549 | 7.60E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene95596 | -0.828312 | 9.64E-55 | -1.69E-46 |
| MS.gene055748 | MS.gene95596 | -0.828846 | 7.16E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene95596 | -0.813424 | 2.58E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene95596 | -0.827361 | 1.63E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene95596 | -0.839979 | 1.15E-57 | -1.69E-46 |
| MS.gene056224 | MS.gene95596 | 0.820552 | 6.46E-53 | -1.69E-46 |
| MS.gene056359 | MS.gene95596 | 0.808734 | 2.68E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene95596 | -0.84074 | 7.25E-58 | -1.69E-46 |
| MS.gene056590 | MS.gene95596 | 0.816278 | 6.00E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene95596 | 0.815456 | 9.16E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene95596 | -0.808514 | 2.98E-50 | -1.69E-46 |
| MS.gene056688 | MS.gene95596 | 0.805639 | 1.21E-49 | -1.69E-46 |
| MS.gene056692 | MS.gene95596 | -0.819875 | 9.23E-53 | -1.69E-46 |
| MS.gene056694 | MS.gene95596 | 0.815736 | 7.94E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene95596 | 0.834203 | 3.43E-56 | -1.69E-46 |
| MS.gene056856 | MS.gene95596 | 0.864982 | 8.21E-65 | -1.69E-46 |
| MS.gene056857 | MS.gene95596 | 0.810239 | 1.27E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene95596 | 0.802644 | 5.09E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene95596 | 0.83033 | 3.12E-55 | -1.69E-46 |
| MS.gene057544 | MS.gene95596 | 0.811699 | 6.14E-51 | -1.69E-46 |
| MS.gene057658 | MS.gene95596 | -0.809873 | 1.53E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene95596 | 0.835174 | 1.96E-56 | -1.69E-46 |
| MS.gene058158 | MS.gene95596 | 0.808936 | 2.42E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene95596 | 0.800616 | 1.33E-48 | -1.69E-46 |
| MS.gene058268 | MS.gene95596 | 0.803219 | 3.87E-49 | -1.69E-46 |
| MS.gene058269 | MS.gene95596 | 0.804291 | 2.32E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene95596 | 0.832274 | 1.04E-55 | -1.69E-46 |
| MS.gene058338 | MS.gene95596 | -0.813986 | 1.94E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene95596 | -0.827616 | 1.42E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene95596 | -0.827511 | 1.50E-54 | -1.69E-46 |
| MS.gene058544 | MS.gene95596 | -0.80907 | 2.27E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene95596 | 0.809688 | 1.67E-50 | -1.69E-46 |
| MS.gene058723 | MS.gene95596 | -0.801565 | 8.48E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene95596 | -0.836491 | 9.07E-57 | -1.69E-46 |
| MS.gene059022 | MS.gene95596 | -0.819468 | 1.14E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene95596 | -0.834681 | 2.60E-56 | -1.69E-46 |
| MS.gene059315 | MS.gene95596 | 0.810936 | 8.99E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene95596 | -0.804489 | 2.11E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene95596 | -0.816694 | 4.85E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene95596 | -0.802774 | 4.78E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene95596 | -0.826958 | 2.04E-54 | -1.69E-46 |
| MS.gene059953 | MS.gene95596 | 0.809816 | 1.57E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene95596 | 0.836358 | 9.80E-57 | -1.69E-46 |
| MS.gene06000 | MS.gene95596 | -0.832162 | 1.11E-55 | -1.69E-46 |
| MS.gene060087 | MS.gene95596 | 0.810543 | 1.09E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene95596 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene95596 | 0.825172 | 5.42E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene95596 | -0.820096 | 8.22E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene95596 | -0.827066 | 1.92E-54 | -1.69E-46 |
| MS.gene060823 | MS.gene95596 | 0.812128 | 4.95E-51 | -1.69E-46 |
| MS.gene06084 | MS.gene95596 | 0.810952 | 8.92E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene95596 | -0.827803 | 1.28E-54 | -1.69E-46 |
| MS.gene061044 | MS.gene95596 | 0.815343 | 9.71E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene95596 | -0.808731 | 2.68E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95596.t1 | MTR_7g068500 | 86.071 | 280 | 39 | 0 | 1 | 280 | 1 | 280 | 0.0 | 509 |
| MS.gene95596.t1 | MTR_7g068460 | 85.393 | 267 | 39 | 0 | 14 | 280 | 15 | 281 | 2.05e-174 | 483 |
| MS.gene95596.t1 | MTR_7g068470 | 87.149 | 249 | 31 | 1 | 32 | 280 | 2 | 249 | 1.27e-163 | 454 |
| MS.gene95596.t1 | MTR_7g068470 | 85.944 | 249 | 30 | 2 | 32 | 280 | 2 | 245 | 1.80e-159 | 443 |
| MS.gene95596.t1 | MTR_4g016510 | 67.600 | 250 | 79 | 2 | 32 | 279 | 6 | 255 | 6.40e-125 | 357 |
| MS.gene95596.t1 | MTR_7g068530 | 68.400 | 250 | 76 | 3 | 31 | 279 | 5 | 252 | 1.10e-123 | 353 |
| MS.gene95596.t1 | MTR_7g068450 | 66.008 | 253 | 81 | 3 | 32 | 279 | 2 | 254 | 5.87e-120 | 344 |
| MS.gene95596.t1 | MTR_3g070460 | 58.893 | 253 | 102 | 2 | 29 | 279 | 3 | 255 | 6.84e-111 | 321 |
| MS.gene95596.t1 | MTR_7g068550 | 61.506 | 239 | 90 | 2 | 32 | 269 | 6 | 243 | 5.96e-106 | 308 |
| MS.gene95596.t1 | MTR_7g068520 | 52.610 | 249 | 21 | 2 | 32 | 280 | 3 | 154 | 1.02e-77 | 233 |
| MS.gene95596.t1 | MTR_4g016500 | 45.909 | 220 | 49 | 4 | 60 | 279 | 277 | 426 | 4.02e-53 | 179 |
| MS.gene95596.t1 | MTR_2g015500 | 34.434 | 212 | 129 | 4 | 32 | 233 | 4 | 215 | 2.91e-41 | 142 |
| MS.gene95596.t1 | MTR_2g015510 | 34.597 | 211 | 130 | 4 | 31 | 233 | 4 | 214 | 6.67e-41 | 142 |
| MS.gene95596.t1 | MTR_4g105680 | 31.100 | 209 | 133 | 4 | 54 | 256 | 54 | 257 | 1.56e-28 | 110 |
| MS.gene95596.t1 | MTR_2g015480 | 29.534 | 193 | 112 | 8 | 50 | 233 | 76 | 253 | 1.09e-20 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95596.t1 | AT3G11210 | 58.964 | 251 | 101 | 2 | 31 | 279 | 5 | 255 | 1.52e-111 | 322 |
| MS.gene95596.t1 | AT2G38180 | 60.262 | 229 | 81 | 4 | 32 | 257 | 6 | 227 | 3.50e-99 | 293 |
| MS.gene95596.t1 | AT5G45920 | 39.713 | 209 | 119 | 4 | 32 | 233 | 3 | 211 | 1.02e-46 | 157 |
| MS.gene95596.t1 | AT5G45920 | 39.713 | 209 | 119 | 4 | 32 | 233 | 3 | 211 | 1.02e-46 | 157 |
| MS.gene95596.t1 | AT5G62930 | 34.914 | 232 | 140 | 4 | 32 | 256 | 2 | 229 | 4.05e-46 | 155 |
| MS.gene95596.t1 | AT5G62930 | 34.599 | 237 | 144 | 4 | 27 | 256 | 23 | 255 | 7.03e-46 | 155 |
| MS.gene95596.t1 | AT5G45920 | 40.000 | 185 | 104 | 4 | 32 | 209 | 3 | 187 | 5.57e-40 | 138 |
| MS.gene95596.t1 | AT5G45920 | 40.000 | 185 | 104 | 4 | 32 | 209 | 3 | 187 | 5.57e-40 | 138 |
| MS.gene95596.t1 | AT5G62930 | 30.682 | 176 | 107 | 5 | 94 | 263 | 7 | 173 | 4.34e-25 | 99.0 |
Find 61 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAATGCCAGCTGAATT+TGG | 0.274855 | 7.4:+35123798 | MS.gene95596:CDS |
| AATGAGCTCAGTGATGAATT+TGG | 0.285385 | 7.4:+35123447 | MS.gene95596:intron |
| AAGGCGTGCTGTGCAAGTTT+TGG | 0.302736 | 7.4:+35122570 | MS.gene95596:CDS |
| TATGATATGGTTTCTCTTGA+TGG | 0.317939 | 7.4:+35123834 | MS.gene95596:CDS |
| AGAAGATTCACCTTATGATA+TGG | 0.335446 | 7.4:+35123821 | MS.gene95596:CDS |
| CGATCAACGAGGCACAAATT+AGG | 0.337436 | 7.4:+35123324 | MS.gene95596:CDS |
| AATATATTCAGAAGCGTGTT+TGG | 0.360952 | 7.4:+35123500 | MS.gene95596:CDS |
| TGGTCCTCATGTACCATTGC+AGG | 0.370383 | 7.4:+35122917 | MS.gene95596:CDS |
| GTTCAATACAGTTGCTATGA+AGG | 0.383262 | 7.4:+35122341 | MS.gene95596:CDS |
| AGGCCTAAAATTGTGTTGTT+TGG | 0.388286 | 7.4:+35122308 | MS.gene95596:CDS |
| GAAGATTGCTACTCATATAA+TGG | 0.403964 | 7.4:+35122959 | MS.gene95596:CDS |
| ATACAGTTGCTATGAAGGTT+GGG | 0.406406 | 7.4:+35122346 | MS.gene95596:CDS |
| AGTGGGAACCAAGTCTATAT+TGG | 0.407643 | 7.4:+35123775 | MS.gene95596:CDS |
| GGACCAAGGCCACTTGGATA+TGG | 0.410264 | 7.4:-35122900 | None:intergenic |
| CGTCCATATCCAAGTGGCCT+TGG | 0.417610 | 7.4:+35122897 | MS.gene95596:CDS |
| CACTCTACATCTTGAGGAAA+AGG | 0.437938 | 7.4:-35123885 | None:intergenic |
| TGCCATTGAGCAACCATCAT+TGG | 0.437939 | 7.4:+35122842 | MS.gene95596:CDS |
| GTGTCGTGAGATGAAAATAA+AGG | 0.443191 | 7.4:+35123527 | MS.gene95596:CDS |
| ATGAGCTCAGTGATGAATTT+GGG | 0.445759 | 7.4:+35123448 | MS.gene95596:intron |
| AATACAGTTGCTATGAAGGT+TGG | 0.465220 | 7.4:+35122345 | MS.gene95596:CDS |
| ACCTTTCAATCCTCTCTCTC+TGG | 0.465769 | 7.4:+35122162 | MS.gene95596:CDS |
| CTAATTTGTGCCTCGTTGAT+CGG | 0.468483 | 7.4:-35123323 | None:intergenic |
| AAGGTTGGGGAGCAATTAAG+AGG | 0.474474 | 7.4:+35122252 | MS.gene95596:intron |
| TCAAGAGAAACCATATCATA+AGG | 0.483799 | 7.4:-35123831 | None:intergenic |
| ATGATATGGTTTCTCTTGAT+GGG | 0.487292 | 7.4:+35123835 | MS.gene95596:CDS |
| CAATTCAGAAGAGAGATGAT+TGG | 0.494653 | 7.4:+35123568 | MS.gene95596:CDS |
| GATATGCTGCTTGGAATTCA+AGG | 0.495898 | 7.4:+35122551 | MS.gene95596:CDS |
| TTCTCACTTGTACGCTCGTA+AGG | 0.496236 | 7.4:+35122379 | MS.gene95596:CDS |
| GGGTTCTTCCATTGCACAGT+GGG | 0.510655 | 7.4:+35122273 | MS.gene95596:CDS |
| AGGAATACATACAAAATATG+AGG | 0.511040 | 7.4:+35122937 | MS.gene95596:CDS |
| TTGGTCATTGTGTACTTTGG+TGG | 0.513435 | 7.4:+35122861 | MS.gene95596:CDS |
| AAGTACACAATGACCAATGA+TGG | 0.528971 | 7.4:-35122855 | None:intergenic |
| TCATTGGTCATTGTGTACTT+TGG | 0.529767 | 7.4:+35122858 | MS.gene95596:CDS |
| GAATCTTCTCCAAATTCAGC+TGG | 0.530268 | 7.4:-35123807 | None:intergenic |
| ACATGAGGACCAAGGCCACT+TGG | 0.533170 | 7.4:-35122906 | None:intergenic |
| GTTCTTCCATTGCACAGTGG+GGG | 0.534821 | 7.4:+35122275 | MS.gene95596:CDS |
| ACCAGAGAGAGAGGATTGAA+AGG | 0.536372 | 7.4:-35122163 | None:intergenic |
| TAATCCGAGTCTTATGTGAC+AGG | 0.539331 | 7.4:-35123283 | None:intergenic |
| TACAGTTGCTATGAAGGTTG+GGG | 0.558888 | 7.4:+35122347 | MS.gene95596:CDS |
| GAGAAAGTTACCAGAGAGAG+AGG | 0.562172 | 7.4:-35122172 | None:intergenic |
| CAAGGCCACTCTACATCTTG+AGG | 0.564026 | 7.4:-35123891 | None:intergenic |
| AATTGCAGACCACATATCAA+TGG | 0.566695 | 7.4:-35123550 | None:intergenic |
| ATTAACTTCCAATATAGACT+TGG | 0.567442 | 7.4:-35123783 | None:intergenic |
| TGTCATAAAAGAAGCAGAGT+GGG | 0.567830 | 7.4:+35123758 | MS.gene95596:CDS |
| AAATAAAGGCCATTGATATG+TGG | 0.571676 | 7.4:+35123541 | MS.gene95596:CDS |
| GGAGTTCATCTATCAACTGA+GGG | 0.572805 | 7.4:+35123705 | MS.gene95596:CDS |
| GACCAATGATGGTTGCTCAA+TGG | 0.577669 | 7.4:-35122844 | None:intergenic |
| ATTTGTGCCTCGTTGATCGG+AGG | 0.599779 | 7.4:-35123320 | None:intergenic |
| ATCTTGAGGAAAAGGCACAT+TGG | 0.602253 | 7.4:-35123877 | None:intergenic |
| TATTCCTGCAATGGTACATG+AGG | 0.621940 | 7.4:-35122921 | None:intergenic |
| GGGGAGTGAAATATTAACAA+AGG | 0.628715 | 7.4:+35123725 | MS.gene95596:CDS |
| GGTTCTTCCATTGCACAGTG+GGG | 0.662401 | 7.4:+35122274 | MS.gene95596:CDS |
| AGAGCCTGTCACATAAGACT+CGG | 0.665508 | 7.4:+35123279 | MS.gene95596:intron |
| ATGTCATAAAAGAAGCAGAG+TGG | 0.667998 | 7.4:+35123757 | MS.gene95596:CDS |
| TGGAGTTCATCTATCAACTG+AGG | 0.669042 | 7.4:+35123704 | MS.gene95596:CDS |
| AGGGTTCTTCCATTGCACAG+TGG | 0.691611 | 7.4:+35122272 | MS.gene95596:CDS |
| TGTATTCGTCCATATCCAAG+TGG | 0.698167 | 7.4:+35122891 | MS.gene95596:CDS |
| AGGTTGGGGAGCAATTAAGA+GGG | 0.698292 | 7.4:+35122253 | MS.gene95596:intron |
| GAGTTCATCTATCAACTGAG+GGG | 0.728386 | 7.4:+35123706 | MS.gene95596:CDS |
| CAGTAGTCCTCCGATCAACG+AGG | 0.731081 | 7.4:+35123313 | MS.gene95596:CDS |
| GCAATGGTACATGAGGACCA+AGG | 0.776215 | 7.4:-35122914 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTAATTATTGATAATAAA+TGG | - | chr7.4:35123196-35123215 | None:intergenic | 10.0% |
| !!! | AATTTAAAACATTTTAGTTT+AGG | - | chr7.4:35123636-35123655 | None:intergenic | 10.0% |
| !! | AATATGATTGTATTGAAAAA+TGG | - | chr7.4:35122226-35122245 | None:intergenic | 15.0% |
| !! | AGATATATCATAGAATAATT+AGG | - | chr7.4:35123409-35123428 | None:intergenic | 15.0% |
| !! | TCAATAATAATTTAATTACG+TGG | + | chr7.4:35123662-35123681 | MS.gene95596:intron | 15.0% |
| !!! | AAAGATTATACATTTGTTAA+GGG | - | chr7.4:35123373-35123392 | None:intergenic | 15.0% |
| !!! | GTTATTTTGATTTCAAATTT+CGG | + | chr7.4:35122994-35123013 | MS.gene95596:intron | 15.0% |
| !!! | TTTTCAATACAATCATATTA+TGG | + | chr7.4:35122227-35122246 | MS.gene95596:intron | 15.0% |
| !! | AAATCAAAATAATCACATCA+TGG | - | chr7.4:35122768-35122787 | None:intergenic | 20.0% |
| !! | AGTATGAAACTTAATAATTC+AGG | - | chr7.4:35123098-35123117 | None:intergenic | 20.0% |
| !! | ATTACGGTATTAATAATTAG+AGG | - | chr7.4:35122419-35122438 | None:intergenic | 20.0% |
| !!! | CAAAGATTATACATTTGTTA+AGG | - | chr7.4:35123374-35123393 | None:intergenic | 20.0% |
| !!! | TTAATACCGTAATGTTTTAT+TGG | + | chr7.4:35122426-35122445 | MS.gene95596:intron | 20.0% |
| !!! | TTTGGATAAAATTTTTCCTA+AGG | + | chr7.4:35122588-35122607 | MS.gene95596:CDS | 20.0% |
| ! | ACAGAACCAATAAAACATTA+CGG | - | chr7.4:35122435-35122454 | None:intergenic | 25.0% |
| ! | AGGAATACATACAAAATATG+AGG | + | chr7.4:35122937-35122956 | MS.gene95596:CDS | 25.0% |
| ! | ATACAATCATATTATGGTGA+AGG | + | chr7.4:35122233-35122252 | MS.gene95596:intron | 25.0% |
| ! | ATGTTTGTTTCATGTAAGTA+TGG | + | chr7.4:35123595-35123614 | MS.gene95596:intron | 25.0% |
| ! | ATTAACTTCCAATATAGACT+TGG | - | chr7.4:35123786-35123805 | None:intergenic | 25.0% |
| ! | GTCATTATATTCAATACCTT+AGG | - | chr7.4:35122607-35122626 | None:intergenic | 25.0% |
| ! | TTGAATATAATGACTGATGA+AGG | + | chr7.4:35122613-35122632 | MS.gene95596:intron | 25.0% |
| ! | TTGATCCAAAAAATTTCCTA+AGG | + | chr7.4:35122642-35122661 | MS.gene95596:intron | 25.0% |
| ! | TTTAACACAATTGAAATACG+TGG | + | chr7.4:35123031-35123050 | MS.gene95596:intron | 25.0% |
| !! | GTTTGATTATTTCTTAGTGA+TGG | + | chr7.4:35123684-35123703 | MS.gene95596:intron | 25.0% |
| !!! | ATATGCCTTAGGAAATTTTT+TGG | - | chr7.4:35122650-35122669 | None:intergenic | 25.0% |
| AAATAAAGGCCATTGATATG+TGG | + | chr7.4:35123541-35123560 | MS.gene95596:CDS | 30.0% | |
| AATCATATTATGGTGAAGGT+TGG | + | chr7.4:35122237-35122256 | MS.gene95596:intron | 30.0% | |
| ACTTATGTATATGCTGCAAA+TGG | - | chr7.4:35122698-35122717 | None:intergenic | 30.0% | |
| AGAAGATTCACCTTATGATA+TGG | + | chr7.4:35123821-35123840 | MS.gene95596:CDS | 30.0% | |
| ATCATATTATGGTGAAGGTT+GGG | + | chr7.4:35122238-35122257 | MS.gene95596:intron | 30.0% | |
| ATGATATGGTTTCTCTTGAT+GGG | + | chr7.4:35123835-35123854 | MS.gene95596:CDS | 30.0% | |
| GAAGATTGCTACTCATATAA+TGG | + | chr7.4:35122959-35122978 | MS.gene95596:CDS | 30.0% | |
| TATGATATGGTTTCTCTTGA+TGG | + | chr7.4:35123834-35123853 | MS.gene95596:CDS | 30.0% | |
| TCAAGAGAAACCATATCATA+AGG | - | chr7.4:35123834-35123853 | None:intergenic | 30.0% | |
| ! | AATATATTCAGAAGCGTGTT+TGG | + | chr7.4:35123500-35123519 | MS.gene95596:CDS | 30.0% |
| ! | GAACCAAACAACACAATTTT+AGG | - | chr7.4:35122314-35122333 | None:intergenic | 30.0% |
| ! | TACATTTGTTAAGGGAACAA+AGG | - | chr7.4:35123365-35123384 | None:intergenic | 30.0% |
| ! | TTTTGTATGTATTCCTGCAA+TGG | - | chr7.4:35122933-35122952 | None:intergenic | 30.0% |
| !! | ACCTAACATTGATTTCATGT+AGG | + | chr7.4:35122510-35122529 | MS.gene95596:intron | 30.0% |
| AAGTACACAATGACCAATGA+TGG | - | chr7.4:35122858-35122877 | None:intergenic | 35.0% | |
| AAGTTAATGCCAGCTGAATT+TGG | + | chr7.4:35123798-35123817 | MS.gene95596:CDS | 35.0% | |
| AATACAGTTGCTATGAAGGT+TGG | + | chr7.4:35122345-35122364 | MS.gene95596:CDS | 35.0% | |
| AATGAGCTCAGTGATGAATT+TGG | + | chr7.4:35123447-35123466 | MS.gene95596:intron | 35.0% | |
| AATTGCAGACCACATATCAA+TGG | - | chr7.4:35123553-35123572 | None:intergenic | 35.0% | |
| ATACAGTTGCTATGAAGGTT+GGG | + | chr7.4:35122346-35122365 | MS.gene95596:CDS | 35.0% | |
| ATGAGCTCAGTGATGAATTT+GGG | + | chr7.4:35123448-35123467 | MS.gene95596:intron | 35.0% | |
| ATGTCATAAAAGAAGCAGAG+TGG | + | chr7.4:35123757-35123776 | MS.gene95596:CDS | 35.0% | |
| CAATTCAGAAGAGAGATGAT+TGG | + | chr7.4:35123568-35123587 | MS.gene95596:CDS | 35.0% | |
| GACAATGCATAATATATGCC+AGG | - | chr7.4:35122459-35122478 | None:intergenic | 35.0% | |
| GCCTACATGAAATCAATGTT+AGG | - | chr7.4:35122514-35122533 | None:intergenic | 35.0% | |
| GGGGAGTGAAATATTAACAA+AGG | + | chr7.4:35123725-35123744 | MS.gene95596:CDS | 35.0% | |
| GTGTCGTGAGATGAAAATAA+AGG | + | chr7.4:35123527-35123546 | MS.gene95596:CDS | 35.0% | |
| GTTCAATACAGTTGCTATGA+AGG | + | chr7.4:35122341-35122360 | MS.gene95596:CDS | 35.0% | |
| TATGTATATGCTGCAAATGG+TGG | - | chr7.4:35122695-35122714 | None:intergenic | 35.0% | |
| TCATATTATGGTGAAGGTTG+GGG | + | chr7.4:35122239-35122258 | MS.gene95596:intron | 35.0% | |
| TCATTGGTCATTGTGTACTT+TGG | + | chr7.4:35122858-35122877 | MS.gene95596:CDS | 35.0% | |
| TGTCATAAAAGAAGCAGAGT+GGG | + | chr7.4:35123758-35123777 | MS.gene95596:CDS | 35.0% | |
| TTGAGGGTAAAATATGCCTT+AGG | - | chr7.4:35122661-35122680 | None:intergenic | 35.0% | |
| ! | AGGCCTAAAATTGTGTTGTT+TGG | + | chr7.4:35122308-35122327 | MS.gene95596:CDS | 35.0% |
| ! | TGAGCTTAACTTAGTTGGTA+TGG | - | chr7.4:35122481-35122500 | None:intergenic | 35.0% |
| ! | TGGTGGTATTACTTAAGTTG+AGG | - | chr7.4:35122678-35122697 | None:intergenic | 35.0% |
| !! | GGTGGTATTACTTAAGTTGA+GGG | - | chr7.4:35122677-35122696 | None:intergenic | 35.0% |
| !! | TGTAGGCTGATATAGTTTTG+CGG | + | chr7.4:35122527-35122546 | MS.gene95596:intron | 35.0% |
| !!! | TGTTTTATTGGTTCTGTACC+TGG | + | chr7.4:35122438-35122457 | MS.gene95596:intron | 35.0% |
| AGTGGGAACCAAGTCTATAT+TGG | + | chr7.4:35123775-35123794 | MS.gene95596:CDS | 40.0% | |
| CACCTCTGATACTCTTATAC+TGG | + | chr7.4:35122802-35122821 | MS.gene95596:intron | 40.0% | |
| CACTCTACATCTTGAGGAAA+AGG | - | chr7.4:35123888-35123907 | None:intergenic | 40.0% | |
| GAATCTTCTCCAAATTCAGC+TGG | - | chr7.4:35123810-35123829 | None:intergenic | 40.0% | |
| GAGTTCATCTATCAACTGAG+GGG | + | chr7.4:35123706-35123725 | MS.gene95596:CDS | 40.0% | |
| GGAGTTCATCTATCAACTGA+GGG | + | chr7.4:35123705-35123724 | MS.gene95596:CDS | 40.0% | |
| TACAGTTGCTATGAAGGTTG+GGG | + | chr7.4:35122347-35122366 | MS.gene95596:CDS | 40.0% | |
| TATTCCTGCAATGGTACATG+AGG | - | chr7.4:35122924-35122943 | None:intergenic | 40.0% | |
| TGGAGTTCATCTATCAACTG+AGG | + | chr7.4:35123704-35123723 | MS.gene95596:CDS | 40.0% | |
| TGTATTCGTCCATATCCAAG+TGG | + | chr7.4:35122891-35122910 | MS.gene95596:CDS | 40.0% | |
| TTGGTCATTGTGTACTTTGG+TGG | + | chr7.4:35122861-35122880 | MS.gene95596:CDS | 40.0% | |
| ! | ATCTTGAGGAAAAGGCACAT+TGG | - | chr7.4:35123880-35123899 | None:intergenic | 40.0% |
| ! | CTAATTTGTGCCTCGTTGAT+CGG | - | chr7.4:35123326-35123345 | None:intergenic | 40.0% |
| ! | CTTTTCCTCAAGATGTAGAG+TGG | + | chr7.4:35123886-35123905 | MS.gene95596:CDS | 40.0% |
| ! | GATATGCTGCTTGGAATTCA+AGG | + | chr7.4:35122551-35122570 | MS.gene95596:CDS | 40.0% |
| ! | TAATCCGAGTCTTATGTGAC+AGG | - | chr7.4:35123286-35123305 | None:intergenic | 40.0% |
| !! | GACCAGTATAAGAGTATCAG+AGG | - | chr7.4:35122807-35122826 | None:intergenic | 40.0% |
| !! | GTAGGCTGATATAGTTTTGC+GGG | + | chr7.4:35122528-35122547 | MS.gene95596:intron | 40.0% |
| !! | TAGGCTGATATAGTTTTGCG+GGG | + | chr7.4:35122529-35122548 | MS.gene95596:intron | 40.0% |
| AAGGTTGGGGAGCAATTAAG+AGG | + | chr7.4:35122252-35122271 | MS.gene95596:intron | 45.0% | |
| ACCAGAGAGAGAGGATTGAA+AGG | - | chr7.4:35122166-35122185 | None:intergenic | 45.0% | |
| ACCTTTCAATCCTCTCTCTC+TGG | + | chr7.4:35122162-35122181 | MS.gene95596:CDS | 45.0% | |
| AGAGCCTGTCACATAAGACT+CGG | + | chr7.4:35123279-35123298 | MS.gene95596:intron | 45.0% | |
| AGGTTGGGGAGCAATTAAGA+GGG | + | chr7.4:35122253-35122272 | MS.gene95596:intron | 45.0% | |
| CCAACTAAGTTAAGCTCACG+AGG | + | chr7.4:35122483-35122502 | MS.gene95596:intron | 45.0% | |
| CCTCGTGAGCTTAACTTAGT+TGG | - | chr7.4:35122486-35122505 | None:intergenic | 45.0% | |
| CGATCAACGAGGCACAAATT+AGG | + | chr7.4:35123324-35123343 | MS.gene95596:CDS | 45.0% | |
| GACCAATGATGGTTGCTCAA+TGG | - | chr7.4:35122847-35122866 | None:intergenic | 45.0% | |
| GAGAAAGTTACCAGAGAGAG+AGG | - | chr7.4:35122175-35122194 | None:intergenic | 45.0% | |
| TGCCATTGAGCAACCATCAT+TGG | + | chr7.4:35122842-35122861 | MS.gene95596:CDS | 45.0% | |
| TTCTCACTTGTACGCTCGTA+AGG | + | chr7.4:35122379-35122398 | MS.gene95596:CDS | 45.0% | |
| AGGGTTCTTCCATTGCACAG+TGG | + | chr7.4:35122272-35122291 | MS.gene95596:CDS | 50.0% | |
| ATTTGTGCCTCGTTGATCGG+AGG | - | chr7.4:35123323-35123342 | None:intergenic | 50.0% | |
| CAAGGCCACTCTACATCTTG+AGG | - | chr7.4:35123894-35123913 | None:intergenic | 50.0% | |
| GCAATGGTACATGAGGACCA+AGG | - | chr7.4:35122917-35122936 | None:intergenic | 50.0% | |
| GGGTTCTTCCATTGCACAGT+GGG | + | chr7.4:35122273-35122292 | MS.gene95596:CDS | 50.0% | |
| GGTTCTTCCATTGCACAGTG+GGG | + | chr7.4:35122274-35122293 | MS.gene95596:CDS | 50.0% | |
| GTTCTTCCATTGCACAGTGG+GGG | + | chr7.4:35122275-35122294 | MS.gene95596:CDS | 50.0% | |
| TGGTCCTCATGTACCATTGC+AGG | + | chr7.4:35122917-35122936 | MS.gene95596:CDS | 50.0% | |
| ! | AAGGCGTGCTGTGCAAGTTT+TGG | + | chr7.4:35122570-35122589 | MS.gene95596:CDS | 50.0% |
| ! | TTTTGCGGGGATATGCTGCT+TGG | + | chr7.4:35122542-35122561 | MS.gene95596:CDS | 50.0% |
| ACAGTGGGGGAGCTATATCG+AGG | + | chr7.4:35122288-35122307 | MS.gene95596:CDS | 55.0% | |
| ACATGAGGACCAAGGCCACT+TGG | - | chr7.4:35122909-35122928 | None:intergenic | 55.0% | |
| ATAGCTCCCCCACTGTGCAA+TGG | - | chr7.4:35122284-35122303 | None:intergenic | 55.0% | |
| CAGTAGTCCTCCGATCAACG+AGG | + | chr7.4:35123313-35123332 | MS.gene95596:CDS | 55.0% | |
| CGTCCATATCCAAGTGGCCT+TGG | + | chr7.4:35122897-35122916 | MS.gene95596:CDS | 55.0% | |
| GGACCAAGGCCACTTGGATA+TGG | - | chr7.4:35122903-35122922 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 35122144 | 35123914 | 35122144 | ID=MS.gene95596 |
| chr7.4 | mRNA | 35122144 | 35123914 | 35122144 | ID=MS.gene95596.t1;Parent=MS.gene95596 |
| chr7.4 | exon | 35122144 | 35122183 | 35122144 | ID=MS.gene95596.t1.exon1;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35122144 | 35122183 | 35122144 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35122255 | 35122400 | 35122255 | ID=MS.gene95596.t1.exon2;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35122255 | 35122400 | 35122255 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35122532 | 35122609 | 35122532 | ID=MS.gene95596.t1.exon3;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35122532 | 35122609 | 35122532 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35122840 | 35122980 | 35122840 | ID=MS.gene95596.t1.exon4;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35122840 | 35122980 | 35122840 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35123281 | 35123354 | 35123281 | ID=MS.gene95596.t1.exon5;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35123281 | 35123354 | 35123281 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35123458 | 35123607 | 35123458 | ID=MS.gene95596.t1.exon6;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35123458 | 35123607 | 35123458 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| chr7.4 | exon | 35123701 | 35123914 | 35123701 | ID=MS.gene95596.t1.exon7;Parent=MS.gene95596.t1 |
| chr7.4 | CDS | 35123701 | 35123914 | 35123701 | ID=cds.MS.gene95596.t1;Parent=MS.gene95596.t1 |
| Gene Sequence |
| Protein sequence |
