AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001507.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001507.01.T01 MTR_4g006040 98.925 93 1 0 6 98 145 237 1.79e-63 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGATCACCTCTTTCATTC+TGG 0.220450 1:-22388021 MsG0180001507.01.T01:CDS
TCGTGGGATCCGGGCGGTCC+AGG 0.255275 1:-22388194 MsG0180001507.01.T01:CDS
GAGGATAGATGGGGCGATTC+AGG 0.280696 1:-22388250 MsG0180001507.01.T01:CDS
TTCTATTCACTCGTGGGATC+CGG 0.285661 1:-22388204 MsG0180001507.01.T01:CDS
ATATATCCCTTATCAGTATA+TGG 0.331456 1:-22388136 MsG0180001507.01.T01:CDS
TATCTCTCGAGCACAGGTTT+AGG 0.344757 1:-22388108 MsG0180001507.01.T01:CDS
CTTCTCCTCAGGAGGATAGA+TGG 0.346630 1:-22388261 MsG0180001507.01.T01:CDS
TATCCTCCTGAGGAGAAGTT+TGG 0.356300 1:+22388266 None:intergenic
GAGATCACCTCTTTCATTCT+GGG 0.384850 1:-22388020 MsG0180001507.01.T01:CDS
GTGGGATCCGGGCGGTCCAG+GGG 0.385871 1:-22388192 MsG0180001507.01.T01:CDS
TGGGATCCGGGCGGTCCAGG+GGG 0.394291 1:-22388191 MsG0180001507.01.T01:CDS
CTCGAGCACAGGTTTAGGTT+TGG 0.395333 1:-22388103 MsG0180001507.01.T01:CDS
CGTGGGATCCGGGCGGTCCA+GGG 0.409711 1:-22388193 MsG0180001507.01.T01:CDS
AGAGGTGATCTCGTAGTTCT+TGG 0.431705 1:+22388031 None:intergenic
TTCTCCTCAGGAGGATAGAT+GGG 0.436019 1:-22388260 MsG0180001507.01.T01:CDS
TTTCATTCTGGGGTGACGGC+GGG 0.437684 1:-22388009 MsG0180001507.01.T01:CDS
AGCTGTCCATATACTGATAA+GGG 0.448121 1:+22388130 None:intergenic
TAGCTGTCCATATACTGATA+AGG 0.448263 1:+22388129 None:intergenic
TTGAAACCAAACTTCTCCTC+AGG 0.463068 1:-22388272 None:intergenic
TCTATTCACTCGTGGGATCC+GGG 0.481377 1:-22388203 MsG0180001507.01.T01:CDS
GTCTGTGAAGATACGTTGTT+AGG 0.494777 1:+22388053 None:intergenic
GGGATCCGGGCGGTCCAGGG+GGG 0.502113 1:-22388190 MsG0180001507.01.T01:CDS
CCCACGAGTGAATAGAAAGT+TGG 0.515787 1:+22388210 None:intergenic
GACAGCTATCTCTCGAGCAC+AGG 0.526610 1:-22388114 MsG0180001507.01.T01:CDS
TCCAACTTTCTATTCACTCG+TGG 0.529895 1:-22388211 MsG0180001507.01.T01:CDS
CTTTCATTCTGGGGTGACGG+CGG 0.540721 1:-22388010 MsG0180001507.01.T01:CDS
CGAGAGGAGAGGAGCCATGA+TGG 0.546958 1:+22388166 None:intergenic
CCGTCACCCCAGAATGAAAG+AGG 0.549518 1:+22388013 None:intergenic
CCTCTTTCATTCTGGGGTGA+CGG 0.551798 1:-22388013 MsG0180001507.01.T01:CDS
CCCATTTCGAGTCAAGAGAA+TGG 0.558119 1:+22387980 None:intergenic
ATAGAAAGTTGGATCTACAT+TGG 0.570959 1:+22388221 None:intergenic
AAACCAAACTTCTCCTCAGG+AGG 0.577461 1:-22388269 None:intergenic
CCAACTTTCTATTCACTCGT+GGG 0.591847 1:-22388210 MsG0180001507.01.T01:CDS
AGATCACCTCTTTCATTCTG+GGG 0.626680 1:-22388019 MsG0180001507.01.T01:CDS
ATATGAATTCTCGAGAGGAG+AGG 0.651842 1:+22388155 None:intergenic
GGGATATATGAATTCTCGAG+AGG 0.654907 1:+22388150 None:intergenic
TCGCCCCATCTATCCTCCTG+AGG 0.661903 1:+22388256 None:intergenic
TCTCCTCAGGAGGATAGATG+GGG 0.682857 1:-22388259 MsG0180001507.01.T01:CDS
ATTCACTCGTGGGATCCGGG+CGG 0.763324 1:-22388200 MsG0180001507.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATATCCCTTATCAGTATA+TGG - Chr1:22388120-22388139 MsG0180001507.01.T01:CDS 25.0%
! CCTCAATAGAAAAAGAAAAA+CGG - Chr1:22388176-22388195 MsG0180001507.01.T01:CDS 25.0%
ATAGAAAGTTGGATCTACAT+TGG + Chr1:22388038-22388057 None:intergenic 30.0%
!! CCGTTTTTCTTTTTCTATTG+AGG + Chr1:22388179-22388198 None:intergenic 30.0%
AGCTGTCCATATACTGATAA+GGG + Chr1:22388129-22388148 None:intergenic 35.0%
TAGCTGTCCATATACTGATA+AGG + Chr1:22388130-22388149 None:intergenic 35.0%
AGATCACCTCTTTCATTCTG+GGG - Chr1:22388237-22388256 MsG0180001507.01.T01:CDS 40.0%
ATATGAATTCTCGAGAGGAG+AGG + Chr1:22388104-22388123 None:intergenic 40.0%
CCAACTTTCTATTCACTCGT+GGG - Chr1:22388046-22388065 MsG0180001507.01.T01:CDS 40.0%
GAGATCACCTCTTTCATTCT+GGG - Chr1:22388236-22388255 MsG0180001507.01.T01:CDS 40.0%
GGGATATATGAATTCTCGAG+AGG + Chr1:22388109-22388128 None:intergenic 40.0%
GTCTGTGAAGATACGTTGTT+AGG + Chr1:22388206-22388225 None:intergenic 40.0%
TCCAACTTTCTATTCACTCG+TGG - Chr1:22388045-22388064 MsG0180001507.01.T01:CDS 40.0%
AGAGGTGATCTCGTAGTTCT+TGG + Chr1:22388228-22388247 None:intergenic 45.0%
CCCACGAGTGAATAGAAAGT+TGG + Chr1:22388049-22388068 None:intergenic 45.0%
CGAGATCACCTCTTTCATTC+TGG - Chr1:22388235-22388254 MsG0180001507.01.T01:CDS 45.0%
TATCTCTCGAGCACAGGTTT+AGG - Chr1:22388148-22388167 MsG0180001507.01.T01:CDS 45.0%
TTCTATTCACTCGTGGGATC+CGG - Chr1:22388052-22388071 MsG0180001507.01.T01:CDS 45.0%
TTCTCCTCAGGAGGATAGAT+GGG - Chr1:22387996-22388015 MsG0180001507.01.T01:CDS 45.0%
CTTCTCCTCAGGAGGATAGA+TGG - Chr1:22387995-22388014 MsG0180001507.01.T01:CDS 50.0%
TCTATTCACTCGTGGGATCC+GGG - Chr1:22388053-22388072 MsG0180001507.01.T01:CDS 50.0%
TCTCCTCAGGAGGATAGATG+GGG - Chr1:22387997-22388016 MsG0180001507.01.T01:CDS 50.0%
! CTCGAGCACAGGTTTAGGTT+TGG - Chr1:22388153-22388172 MsG0180001507.01.T01:CDS 50.0%
!! CCTCTTTCATTCTGGGGTGA+CGG - Chr1:22388243-22388262 MsG0180001507.01.T01:CDS 50.0%
CCGTCACCCCAGAATGAAAG+AGG + Chr1:22388246-22388265 None:intergenic 55.0%
GACAGCTATCTCTCGAGCAC+AGG - Chr1:22388142-22388161 MsG0180001507.01.T01:CDS 55.0%
GAGGATAGATGGGGCGATTC+AGG - Chr1:22388006-22388025 MsG0180001507.01.T01:CDS 55.0%
!! CTTTCATTCTGGGGTGACGG+CGG - Chr1:22388246-22388265 MsG0180001507.01.T01:CDS 55.0%
!! TTTCATTCTGGGGTGACGGC+GGG - Chr1:22388247-22388266 MsG0180001507.01.T01:CDS 55.0%
ATTCACTCGTGGGATCCGGG+CGG - Chr1:22388056-22388075 MsG0180001507.01.T01:CDS 60.0%
CGAGAGGAGAGGAGCCATGA+TGG + Chr1:22388093-22388112 None:intergenic 60.0%
TCGCCCCATCTATCCTCCTG+AGG + Chr1:22388003-22388022 None:intergenic 60.0%
CGGTCCAGGGGGGACCATCA+TGG - Chr1:22388076-22388095 MsG0180001507.01.T01:CDS 70.0%
GGAGCCATGATGGTCCCCCC+TGG + Chr1:22388083-22388102 None:intergenic 70.0%
ATGGTCCCCCCTGGACCGCC+CGG + Chr1:22388074-22388093 None:intergenic 75.0%
CGTGGGATCCGGGCGGTCCA+GGG - Chr1:22388063-22388082 MsG0180001507.01.T01:CDS 75.0%
GTGGGATCCGGGCGGTCCAG+GGG - Chr1:22388064-22388083 MsG0180001507.01.T01:CDS 75.0%
TCGTGGGATCCGGGCGGTCC+AGG - Chr1:22388062-22388081 MsG0180001507.01.T01:CDS 75.0%
TGGGATCCGGGCGGTCCAGG+GGG - Chr1:22388065-22388084 MsG0180001507.01.T01:CDS 75.0%
! GGGATCCGGGCGGTCCAGGG+GGG - Chr1:22388066-22388085 MsG0180001507.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 22387991 22388287 22387991 ID=MsG0180001507.01;Name=MsG0180001507.01
Chr1 mRNA 22387991 22388287 22387991 ID=MsG0180001507.01.T01;Parent=MsG0180001507.01;Name=MsG0180001507.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|98
Chr1 exon 22387991 22388287 22387991 ID=MsG0180001507.01.T01:exon:19351;Parent=MsG0180001507.01.T01
Chr1 CDS 22387991 22388287 22387991 ID=MsG0180001507.01.T01:cds;Parent=MsG0180001507.01.T01
Chr1 mRNA 22388109 22388183 22388109 ID=MsG0180001507.01.T02;Parent=MsG0180001507.01;Name=MsG0180001507.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|24
Chr1 exon 22388109 22388183 22388109 ID=MsG0180001507.01.T02:exon:19352;Parent=MsG0180001507.01.T02
Chr1 CDS 22388109 22388183 22388109 ID=MsG0180001507.01.T02:cds;Parent=MsG0180001507.01.T02
Gene Sequence

>MsG0180001507.01.T01

CAAACTTCTCCTCAGGAGGATAGATGGGGCGATTCAGGTGAGATCCAATGTAGATCCAACTTTCTATTCACTCGTGGGATCCGGGCGGTCCAGGGGGGACCATCATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAGGTTTGGCCTCAATAGAAAAAGAAAAACGGAGCACCTAACAACGTATCTTCACAGACCAAGAACTACGAGATCACCTCTTTCATTCTGGGGTGACGGCGGGATCGTACCATTCTCTTGA

>MsG0180001507.01.T02

ATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAG

Protein sequence

>MsG0180001507.01.T01

QTSPQEDRWGDSGEIQCRSNFLFTRGIRAVQGGPSWLLSSREFIYPLSVYGQLSLEHRFRFGLNRKRKTEHLTTYLHRPRTTRSPLSFWGDGGIVPFS*

>MsG0180001507.01.T02

MAPLLSRIHISLISIWTAISRAQV*