Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020051.01.T01 | AES69825.1 | 97.959 | 98 | 2 | 0 | 1 | 98 | 1 | 98 | 8.99E-62 | 193 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0480020051.01 | 0.803306 | 3.713292e-49 | 1.859602e-46 |
| MsG0080048059.01 | MsG0480020051.01 | 0.812550 | 4.007190e-51 | 2.555444e-48 |
| MsG0080048611.01 | MsG0480020051.01 | 0.802631 | 5.120149e-49 | 2.520405e-46 |
| MsG0080048633.01 | MsG0480020051.01 | -0.824182 | 9.271803e-54 | 8.152762e-51 |
| MsG0080048652.01 | MsG0480020051.01 | -0.823140 | 1.626020e-53 | 1.388426e-50 |
| MsG0080048691.01 | MsG0480020051.01 | 0.815992 | 6.955726e-52 | 4.869039e-49 |
| MsG0080048694.01 | MsG0480020051.01 | 0.905824 | 2.851415e-80 | 4.694883e-76 |
| MsG0080048695.01 | MsG0480020051.01 | 0.906246 | 1.820530e-80 | 3.055239e-76 |
| MsG0080048697.01 | MsG0480020051.01 | 0.877318 | 6.915867e-69 | 3.462632e-65 |
| MsG0080048887.01 | MsG0480020051.01 | 0.816352 | 5.779167e-52 | 4.085576e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020051.01.T01 | MTR_3g035620 | 97.959 | 98 | 2 | 0 | 1 | 98 | 1 | 98 | 1.09e-65 | 193 |
| MsG0480020051.01.T01 | MTR_0002s0250 | 97.959 | 98 | 2 | 0 | 1 | 98 | 116 | 213 | 2.87e-65 | 196 |
Find 64 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCGATTCCCGTAGTAG+CGG | 0.670785 | 4:+32893312 | MsG0480020051.01.T01:exon |
| AAACCGTGAAAACGGGGTTG+TGG | 0.519102 | 4:+32893356 | MsG0480020051.01.T01:three_prime_UTR |
| AAACGAGGAAAGGCTTATGG+TGG | 0.638319 | 4:+32893105 | MsG0480020051.01.T01:CDS |
| AACAGCCTAAACCGTGAAAA+CGG | 0.420484 | 4:+32893348 | MsG0480020051.01.T01:three_prime_UTR |
| AACCGTGAAAACGGGGTTGT+GGG | 0.493453 | 4:+32893357 | MsG0480020051.01.T01:three_prime_UTR |
| AAGGCTTATGGTGGATACCT+AGG | 0.574482 | 4:+32893114 | MsG0480020051.01.T01:CDS |
| ACAGCCTAAACCGTGAAAAC+GGG | 0.421801 | 4:+32893349 | MsG0480020051.01.T01:three_prime_UTR |
| ACTACGCCCTTCCTCGTCTC+TGG | 0.292452 | 4:-32893140 | None:intergenic |
| AGATCCGAGGATTCCCGAAT+AGG | 0.444375 | 4:+32893200 | MsG0480020051.01.T01:CDS |
| AGGCACCCAGAGACGAGGAA+GGG | 0.625622 | 4:+32893134 | MsG0480020051.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAAATAAGCATAGATCCG+AGG | + | Chr4:32893187-32893206 | MsG0480020051.01.T01:CDS | 35.0% | |
| TTGAAGTTTATGCCATCCTA+AGG | - | Chr4:32893069-32893088 | None:intergenic | 35.0% | |
| AACAGCCTAAACCGTGAAAA+CGG | + | Chr4:32893348-32893367 | MsG0480020051.01.T01:three_prime_UTR | 40.0% | |
| GCATAAGCTAGTGATGCTTT+CGG | - | Chr4:32893455-32893474 | None:intergenic | 40.0% | |
| GTTCGAAAGGTTGACCTATT+CGG | - | Chr4:32893217-32893236 | None:intergenic | 40.0% | |
| TACTGGACTTTCACCATCTA+GGG | - | Chr4:32893431-32893450 | None:intergenic | 40.0% | |
| TAGTAAGCGACGAAATGCTT+CGG | + | Chr4:32893159-32893178 | MsG0480020051.01.T01:CDS | 40.0% | |
| TGAAACATCTTAGTAGCCAG+AGG | + | Chr4:32893279-32893298 | MsG0480020051.01.T01:CDS | 40.0% | |
| TTCAAACGAGGAAAGGCTTA+TGG | + | Chr4:32893102-32893121 | MsG0480020051.01.T01:CDS | 40.0% | |
| TTCGAAAGGTTGACCTATTC+GGG | - | Chr4:32893216-32893235 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 32893032 | 32893572 | 32893032 | ID=MsG0480020051.01;Name=MsG0480020051.01 |
| Chr4 | mRNA | 32893032 | 32893572 | 32893032 | ID=MsG0480020051.01.T01;Parent=MsG0480020051.01;Name=MsG0480020051.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|244|98 |
| Chr4 | exon | 32893032 | 32893572 | 32893032 | ID=MsG0480020051.01.T01:exon:6022;Parent=MsG0480020051.01.T01 |
| Chr4 | CDS | 32893032 | 32893328 | 32893032 | ID=MsG0480020051.01.T01:cds;Parent=MsG0480020051.01.T01 |
| Chr4 | three_prime_UTR | 32893329 | 32893572 | 32893329 | ID=MsG0480020051.01.T01:three_prime_utr;Parent=MsG0480020051.01.T01 |
| Gene Sequence |
>MsG0480020051.01.T01 ATGGACGTTGATAAGATCCTTCCATCTAGCAGCACCTTAGGATGGCATAAACTTCAAGTTAAGGGCGAGGTTCAAACGAGGAAAGGCTTATGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGATCCGAGGATTCCCGAATAGGTCAACCTTTCGAACTGCTGCTGAATTCACGAGCAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTAG |
| Protein sequence |
>MsG0480020051.01.T01 MDVDKILPSSSTLGWHKLQVKGEVQTRKGLWWIPRHPETRKGVVSDEMLRGVENKHRSEDSRIGQPFELLLNSRAGKRQPGELKHLSSQRKRKQKRFP* |
