AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013984.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013984.01.T01 MTR_4g006040 98.925 93 1 0 6 98 145 237 1.79e-63 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGATCACCTCTTTCATTC+TGG 0.220450 3:+48574591 MsG0380013984.01.T01:CDS
TCGTGGGATCCGGGCGGTCC+AGG 0.255275 3:+48574418 MsG0380013984.01.T01:CDS
GAGGATAGATGGGGCGATTC+AGG 0.280696 3:+48574362 MsG0380013984.01.T01:CDS
TTCTATTCACTCGTGGGATC+CGG 0.285661 3:+48574408 MsG0380013984.01.T01:CDS
ATATATCCCTTATCAGTATA+TGG 0.331456 3:+48574476 MsG0380013984.01.T01:CDS
TATCTCTCGAGCACAGGTTT+AGG 0.344757 3:+48574504 MsG0380013984.01.T01:CDS
CTTCTCCTCAGGAGGATAGA+TGG 0.346630 3:+48574351 MsG0380013984.01.T01:CDS
TATCCTCCTGAGGAGAAGTT+TGG 0.356300 3:-48574346 None:intergenic
GAGATCACCTCTTTCATTCT+GGG 0.384850 3:+48574592 MsG0380013984.01.T01:CDS
GTGGGATCCGGGCGGTCCAG+GGG 0.385871 3:+48574420 MsG0380013984.01.T01:CDS
TGGGATCCGGGCGGTCCAGG+GGG 0.394291 3:+48574421 MsG0380013984.01.T01:CDS
CTCGAGCACAGGTTTAGGTT+TGG 0.395333 3:+48574509 MsG0380013984.01.T01:CDS
CGTGGGATCCGGGCGGTCCA+GGG 0.409711 3:+48574419 MsG0380013984.01.T01:CDS
AGAGGTGATCTCGTAGTTCT+TGG 0.431705 3:-48574581 None:intergenic
TTCTCCTCAGGAGGATAGAT+GGG 0.436019 3:+48574352 MsG0380013984.01.T01:CDS
TTTCATTCTGGGGTGACGGC+GGG 0.437684 3:+48574603 MsG0380013984.01.T01:CDS
AGCTGTCCATATACTGATAA+GGG 0.448121 3:-48574482 None:intergenic
TAGCTGTCCATATACTGATA+AGG 0.448263 3:-48574483 None:intergenic
TTGAAACCAAACTTCTCCTC+AGG 0.463068 3:+48574340 None:intergenic
TCTATTCACTCGTGGGATCC+GGG 0.481377 3:+48574409 MsG0380013984.01.T01:CDS
GTCTGTGAAGATACGTTGTT+AGG 0.494777 3:-48574559 None:intergenic
GGGATCCGGGCGGTCCAGGG+GGG 0.502113 3:+48574422 MsG0380013984.01.T01:CDS
CCCACGAGTGAATAGAAAGT+TGG 0.515787 3:-48574402 None:intergenic
GACAGCTATCTCTCGAGCAC+AGG 0.526610 3:+48574498 MsG0380013984.01.T01:CDS
TCCAACTTTCTATTCACTCG+TGG 0.529895 3:+48574401 MsG0380013984.01.T01:CDS
CTTTCATTCTGGGGTGACGG+CGG 0.540721 3:+48574602 MsG0380013984.01.T01:CDS
CGAGAGGAGAGGAGCCATGA+TGG 0.546958 3:-48574446 None:intergenic
CCGTCACCCCAGAATGAAAG+AGG 0.549518 3:-48574599 None:intergenic
CCTCTTTCATTCTGGGGTGA+CGG 0.551798 3:+48574599 MsG0380013984.01.T01:CDS
CCCATTTCGAGTCAAGAGAA+TGG 0.558119 3:-48574632 None:intergenic
ATAGAAAGTTGGATCTACAT+TGG 0.570959 3:-48574391 None:intergenic
AAACCAAACTTCTCCTCAGG+AGG 0.577461 3:+48574343 None:intergenic
CCAACTTTCTATTCACTCGT+GGG 0.591847 3:+48574402 MsG0380013984.01.T01:CDS
AGATCACCTCTTTCATTCTG+GGG 0.626680 3:+48574593 MsG0380013984.01.T01:CDS
ATATGAATTCTCGAGAGGAG+AGG 0.651842 3:-48574457 None:intergenic
GGGATATATGAATTCTCGAG+AGG 0.654907 3:-48574462 None:intergenic
TCGCCCCATCTATCCTCCTG+AGG 0.661903 3:-48574356 None:intergenic
TCTCCTCAGGAGGATAGATG+GGG 0.682857 3:+48574353 MsG0380013984.01.T01:CDS
ATTCACTCGTGGGATCCGGG+CGG 0.763324 3:+48574412 MsG0380013984.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATATCCCTTATCAGTATA+TGG + Chr3:48574476-48574495 MsG0380013984.01.T01:CDS 25.0%
! CCTCAATAGAAAAAGAAAAA+CGG + Chr3:48574532-48574551 MsG0380013984.01.T01:CDS 25.0%
ATAGAAAGTTGGATCTACAT+TGG - Chr3:48574394-48574413 None:intergenic 30.0%
!! CCGTTTTTCTTTTTCTATTG+AGG - Chr3:48574535-48574554 None:intergenic 30.0%
AGCTGTCCATATACTGATAA+GGG - Chr3:48574485-48574504 None:intergenic 35.0%
TAGCTGTCCATATACTGATA+AGG - Chr3:48574486-48574505 None:intergenic 35.0%
AGATCACCTCTTTCATTCTG+GGG + Chr3:48574593-48574612 MsG0380013984.01.T01:CDS 40.0%
ATATGAATTCTCGAGAGGAG+AGG - Chr3:48574460-48574479 None:intergenic 40.0%
CCAACTTTCTATTCACTCGT+GGG + Chr3:48574402-48574421 MsG0380013984.01.T01:CDS 40.0%
GAGATCACCTCTTTCATTCT+GGG + Chr3:48574592-48574611 MsG0380013984.01.T01:CDS 40.0%
GGGATATATGAATTCTCGAG+AGG - Chr3:48574465-48574484 None:intergenic 40.0%
GTCTGTGAAGATACGTTGTT+AGG - Chr3:48574562-48574581 None:intergenic 40.0%
TCCAACTTTCTATTCACTCG+TGG + Chr3:48574401-48574420 MsG0380013984.01.T01:CDS 40.0%
AGAGGTGATCTCGTAGTTCT+TGG - Chr3:48574584-48574603 None:intergenic 45.0%
CCCACGAGTGAATAGAAAGT+TGG - Chr3:48574405-48574424 None:intergenic 45.0%
CGAGATCACCTCTTTCATTC+TGG + Chr3:48574591-48574610 MsG0380013984.01.T01:CDS 45.0%
TATCTCTCGAGCACAGGTTT+AGG + Chr3:48574504-48574523 MsG0380013984.01.T01:CDS 45.0%
TTCTATTCACTCGTGGGATC+CGG + Chr3:48574408-48574427 MsG0380013984.01.T01:CDS 45.0%
TTCTCCTCAGGAGGATAGAT+GGG + Chr3:48574352-48574371 MsG0380013984.01.T01:CDS 45.0%
CTTCTCCTCAGGAGGATAGA+TGG + Chr3:48574351-48574370 MsG0380013984.01.T01:CDS 50.0%
TCTATTCACTCGTGGGATCC+GGG + Chr3:48574409-48574428 MsG0380013984.01.T01:CDS 50.0%
TCTCCTCAGGAGGATAGATG+GGG + Chr3:48574353-48574372 MsG0380013984.01.T01:CDS 50.0%
! CTCGAGCACAGGTTTAGGTT+TGG + Chr3:48574509-48574528 MsG0380013984.01.T01:CDS 50.0%
!! CCTCTTTCATTCTGGGGTGA+CGG + Chr3:48574599-48574618 MsG0380013984.01.T01:CDS 50.0%
CCGTCACCCCAGAATGAAAG+AGG - Chr3:48574602-48574621 None:intergenic 55.0%
GACAGCTATCTCTCGAGCAC+AGG + Chr3:48574498-48574517 MsG0380013984.01.T01:CDS 55.0%
GAGGATAGATGGGGCGATTC+AGG + Chr3:48574362-48574381 MsG0380013984.01.T01:CDS 55.0%
!! CTTTCATTCTGGGGTGACGG+CGG + Chr3:48574602-48574621 MsG0380013984.01.T01:CDS 55.0%
!! TTTCATTCTGGGGTGACGGC+GGG + Chr3:48574603-48574622 MsG0380013984.01.T01:CDS 55.0%
ATTCACTCGTGGGATCCGGG+CGG + Chr3:48574412-48574431 MsG0380013984.01.T01:CDS 60.0%
CGAGAGGAGAGGAGCCATGA+TGG - Chr3:48574449-48574468 None:intergenic 60.0%
TCGCCCCATCTATCCTCCTG+AGG - Chr3:48574359-48574378 None:intergenic 60.0%
CGGTCCAGGGGGGACCATCA+TGG + Chr3:48574432-48574451 MsG0380013984.01.T01:CDS 70.0%
GGAGCCATGATGGTCCCCCC+TGG - Chr3:48574439-48574458 None:intergenic 70.0%
ATGGTCCCCCCTGGACCGCC+CGG - Chr3:48574430-48574449 None:intergenic 75.0%
CGTGGGATCCGGGCGGTCCA+GGG + Chr3:48574419-48574438 MsG0380013984.01.T01:CDS 75.0%
GTGGGATCCGGGCGGTCCAG+GGG + Chr3:48574420-48574439 MsG0380013984.01.T01:CDS 75.0%
TCGTGGGATCCGGGCGGTCC+AGG + Chr3:48574418-48574437 MsG0380013984.01.T01:CDS 75.0%
TGGGATCCGGGCGGTCCAGG+GGG + Chr3:48574421-48574440 MsG0380013984.01.T01:CDS 75.0%
! GGGATCCGGGCGGTCCAGGG+GGG + Chr3:48574422-48574441 MsG0380013984.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48574347 48574643 48574347 ID=MsG0380013984.01;Name=MsG0380013984.01
Chr3 mRNA 48574347 48574643 48574347 ID=MsG0380013984.01.T01;Parent=MsG0380013984.01;Name=MsG0380013984.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|98
Chr3 exon 48574347 48574643 48574347 ID=MsG0380013984.01.T01:exon:17949;Parent=MsG0380013984.01.T01
Chr3 CDS 48574347 48574643 48574347 ID=MsG0380013984.01.T01:cds;Parent=MsG0380013984.01.T01
Chr3 mRNA 48574451 48574525 48574451 ID=MsG0380013984.01.T02;Parent=MsG0380013984.01;Name=MsG0380013984.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|24
Chr3 exon 48574451 48574525 48574451 ID=MsG0380013984.01.T02:exon:17950;Parent=MsG0380013984.01.T02
Chr3 CDS 48574451 48574525 48574451 ID=MsG0380013984.01.T02:cds;Parent=MsG0380013984.01.T02
Gene Sequence

>MsG0380013984.01.T02

ATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAG

>MsG0380013984.01.T01

CAAACTTCTCCTCAGGAGGATAGATGGGGCGATTCAGGTGAGATCCAATGTAGATCCAACTTTCTATTCACTCGTGGGATCCGGGCGGTCCAGGGGGGACCATCATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAGGTTTGGCCTCAATAGAAAAAGAAAAACGGAGCACCTAACAACGTATCTTCACAGACCAAGAACTACGAGATCACCTCTTTCATTCTGGGGTGACGGCGGGATCGTACCATTCTCTTGA

Protein sequence

>MsG0380013984.01.T02

MAPLLSRIHISLISIWTAISRAQV*

>MsG0380013984.01.T01

QTSPQEDRWGDSGEIQCRSNFLFTRGIRAVQGGPSWLLSSREFIYPLSVYGQLSLEHRFRFGLNRKRKTEHLTTYLHRPRTTRSPLSFWGDGGIVPFS*