Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032066.01.T01 | AES75284.2 | 76.54 | 341 | 75 | 2 | 2 | 342 | 19 | 354 | 0 | 529 |
| MsG0680032066.01.T01 | AES75284.2 | 58.642 | 162 | 63 | 2 | 19 | 178 | 208 | 367 | 1.40E-54 | 194 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032065.01 | MsG0680032066.01 | 0.832137 | 1.122336e-55 | 1.243242e-52 |
| MsG0680032066.01 | MsG0680032070.01 | 0.821910 | 3.140316e-53 | 2.589159e-50 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032066.01.T01 | MTR_6g036510 | 76.540 | 341 | 75 | 2 | 2 | 342 | 19 | 354 | 0.0 | 529 |
| MsG0680032066.01.T01 | MTR_6g036510 | 58.642 | 162 | 63 | 2 | 19 | 178 | 208 | 367 | 1.70e-58 | 194 |
| MsG0680032066.01.T01 | MTR_6g036500 | 82.353 | 306 | 52 | 1 | 11 | 314 | 1 | 306 | 0.0 | 518 |
| MsG0680032066.01.T01 | MTR_6g036500 | 67.785 | 149 | 46 | 1 | 196 | 342 | 9 | 157 | 2.53e-59 | 193 |
| MsG0680032066.01.T01 | MTR_4g080060 | 62.304 | 191 | 69 | 2 | 9 | 196 | 11 | 201 | 5.72e-71 | 239 |
| MsG0680032066.01.T01 | MTR_4g080060 | 68.243 | 148 | 45 | 1 | 197 | 342 | 22 | 169 | 2.81e-58 | 203 |
| MsG0680032066.01.T01 | MTR_4g080070 | 62.304 | 191 | 69 | 2 | 9 | 196 | 11 | 201 | 1.17e-70 | 238 |
| MsG0680032066.01.T01 | MTR_4g080070 | 68.919 | 148 | 44 | 1 | 197 | 342 | 22 | 169 | 8.24e-59 | 204 |
| MsG0680032066.01.T01 | MTR_4g080330 | 62.842 | 183 | 65 | 2 | 20 | 199 | 5 | 187 | 7.31e-70 | 236 |
| MsG0680032066.01.T01 | MTR_4g080330 | 68.000 | 150 | 46 | 1 | 195 | 342 | 3 | 152 | 2.34e-61 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032066.01.T01 | AT1G27180 | 36.119 | 335 | 185 | 8 | 7 | 335 | 4 | 315 | 5.90e-63 | 217 |
| MsG0680032066.01.T01 | AT1G27180 | 41.667 | 144 | 79 | 3 | 20 | 160 | 176 | 317 | 3.35e-29 | 119 |
| MsG0680032066.01.T01 | AT5G36930 | 45.946 | 148 | 79 | 1 | 196 | 342 | 13 | 160 | 6.38e-38 | 144 |
| MsG0680032066.01.T01 | AT5G36930 | 43.678 | 174 | 84 | 5 | 20 | 185 | 14 | 181 | 6.66e-34 | 132 |
| MsG0680032066.01.T01 | AT5G36930 | 45.946 | 148 | 79 | 1 | 196 | 342 | 13 | 160 | 6.38e-38 | 144 |
| MsG0680032066.01.T01 | AT5G36930 | 43.678 | 174 | 84 | 5 | 20 | 185 | 14 | 181 | 6.66e-34 | 132 |
| MsG0680032066.01.T01 | AT5G36930 | 45.946 | 148 | 79 | 1 | 196 | 342 | 16 | 163 | 6.69e-38 | 144 |
| MsG0680032066.01.T01 | AT5G36930 | 43.678 | 174 | 84 | 5 | 20 | 185 | 17 | 184 | 6.92e-34 | 132 |
| MsG0680032066.01.T01 | AT5G36930 | 45.946 | 148 | 79 | 1 | 196 | 342 | 13 | 160 | 6.96e-38 | 144 |
| MsG0680032066.01.T01 | AT5G36930 | 43.678 | 174 | 84 | 5 | 20 | 185 | 14 | 181 | 7.01e-34 | 132 |
Find 59 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGTGAATTCATAGCATC+CGG | 0.510982 | 6:-33017510 | MsG0680032066.01.T01:CDS |
| AACTTGCAGGCCACAACACG+CGG | 0.604815 | 6:-33017730 | MsG0680032066.01.T01:intron |
| AACTTGGTGCTTGCGAGAAT+TGG | 0.285119 | 6:-33017421 | MsG0680032066.01.T01:CDS |
| AATTTCACTGATCATCTTTA+TGG | 0.075505 | 6:-33018338 | MsG0680032066.01.T01:CDS |
| ACTCAAATAAGGTGCAAAGA+TGG | 0.498129 | 6:-33018019 | MsG0680032066.01.T01:CDS |
| ACTCAAATACAGTGCAAGGA+TGG | 0.555169 | 6:-33017266 | MsG0680032066.01.T01:CDS |
| AGAATTATGCTTCTTCAACT+TGG | 0.476893 | 6:-33017437 | MsG0680032066.01.T01:CDS |
| AGAGAAACTCTACAACTAGT+GGG | 0.624157 | 6:-33017996 | MsG0680032066.01.T01:CDS |
| AGAGAAACTCTCACACAAGT+GGG | 0.591432 | 6:-33017243 | MsG0680032066.01.T01:CDS |
| AGCGGAAGAGCTGCTACTGC+TGG | 0.446175 | 6:+33018433 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAATTATGCTTCTTCAACT+TGG | - | Chr6:33018209-33018228 | MsG0680032066.01.T01:CDS | 30.0% | |
| ATACCAAGATGACTCAAATA+AGG | - | Chr6:33017616-33017635 | MsG0680032066.01.T01:CDS | 30.0% | |
| GATGACACTAAACTTGAAAA+AGG | - | Chr6:33018118-33018137 | MsG0680032066.01.T01:CDS | 30.0% | |
| GGATCAACATCATAAAAAAC+AGG | + | Chr6:33017536-33017555 | None:intergenic | 30.0% | |
| TCATAATCATTCTTTCTCCT+TGG | + | Chr6:33017251-33017270 | None:intergenic | 30.0% | |
| TTCAGAGATGAACTTCAAAA+AGG | - | Chr6:33017359-33017378 | MsG0680032066.01.T01:CDS | 30.0% | |
| AAAGGTGAATTCATAGCATC+CGG | - | Chr6:33018136-33018155 | MsG0680032066.01.T01:CDS | 35.0% | |
| AAGTAAGCAATCTTCCCTAT+TGG | - | Chr6:33017701-33017720 | MsG0680032066.01.T01:CDS | 35.0% | |
| ACTCAAATAAGGTGCAAAGA+TGG | - | Chr6:33017627-33017646 | MsG0680032066.01.T01:CDS | 35.0% | |
| AGAGAAACTCTACAACTAGT+GGG | - | Chr6:33017650-33017669 | MsG0680032066.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 33017209 | 33018459 | 33017209 | ID=MsG0680032066.01;Name=MsG0680032066.01 |
| Chr6 | mRNA | 33017209 | 33018459 | 33017209 | ID=MsG0680032066.01.T01;Parent=MsG0680032066.01;Name=MsG0680032066.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|342 |
| Chr6 | exon | 33017966 | 33018459 | 33017966 | ID=MsG0680032066.01.T01:exon:18270;Parent=MsG0680032066.01.T01 |
| Chr6 | exon | 33017209 | 33017743 | 33017209 | ID=MsG0680032066.01.T01:exon:18269;Parent=MsG0680032066.01.T01 |
| Chr6 | CDS | 33017966 | 33018459 | 33017966 | ID=MsG0680032066.01.T01:cds;Parent=MsG0680032066.01.T01 |
| Chr6 | CDS | 33017209 | 33017743 | 33017209 | ID=MsG0680032066.01.T01:cds;Parent=MsG0680032066.01.T01 |
| Gene Sequence |
>MsG0680032066.01.T01 ATGGCCAGCAGTAGCAGCTCTTCCGCTCTAGTGACATCTCCAAGGAGAAAGAATGATTATGACGTGTTTGTGAGCTTCAGAGGCAGAGACACACGCTTGAATTTCACTGATCATCTTTATGGTGCCCTTCAAAGAAAAAAAATTGTTACATTCAGAGATGAACTTCAAAAAGGTGAATCCGTAGTATCCGAACTCTTTCGCGCAATTGAAGGCTCTCAGGTTTTCATAGTTGTATTCTCTCCGAAATATGCTGCTTCATCTTGGTGCTTGCAAGAATTAGCGTACATCTTTAACTGTAGTGTATTGTATGGAAAACGCGTTCTGCCTGTTTTTTATGATGTTGATCCTTCTGAGGTGCGAAAGCAAAATGGTGGTTATGGTGAATCCCTTGCCAAACTTGGAGAGAGATACCAAGATGACTCAAATAAGGTGCAAAGATGGAGAGAAACTCTACAACTAGTGGGTAATATCGTTGGCTGGGATCTATGTCATAAGCCACAACACGCGGAGCTTGAAAACATCATTGAACATATAAATATTCTGGGTTGCAAGTTTCCAAGTCGTACAAAAGATTTGGTTGAGATTAATTATGACGTGTTTGTGAGCTTTAGAGGCCTAGACACACGCTCCAATTTCACCAATGATCTTTTTGCTGCTCTACAAAGAAGAGGCATTAATGCATTCAGAGATGACACTAAACTTGAAAAAGGTGAATTCATAGCATCCGGACTCTCGAAAGCAATTGAAGCCTCTCAGGTTTACATTGTTGTATTCTCTAAGAATTATGCTTCTTCAACTTGGTGCTTGCGAGAATTGGAATACATCCTTCATTGCAGTCAAAAACATGGAAAACGTGTTCTTCCTGTTTTCTATGATGTTGATCCTTCTGAGGTGCAAAAGCAAAGTGGTGGCTATGCTGAAGCCCTATCCAAACATGGATTCGAACATGACTCAAATACAGTGCAAGGATGGAGAGAAACTCTCACACAAGTGGGGAATATCCCTAGCTTGAATCTACGTCATAAGTAA |
| Protein sequence |
>MsG0680032066.01.T01 MASSSSSSALVTSPRRKNDYDVFVSFRGRDTRLNFTDHLYGALQRKKIVTFRDELQKGESVVSELFRAIEGSQVFIVVFSPKYAASSWCLQELAYIFNCSVLYGKRVLPVFYDVDPSEVRKQNGGYGESLAKLGERYQDDSNKVQRWRETLQLVGNIVGWDLCHKPQHAELENIIEHINILGCKFPSRTKDLVEINYDVFVSFRGLDTRSNFTNDLFAALQRRGINAFRDDTKLEKGEFIASGLSKAIEASQVYIVVFSKNYASSTWCLRELEYILHCSQKHGKRVLPVFYDVDPSEVQKQSGGYAEALSKHGFEHDSNTVQGWRETLTQVGNIPSLNLRHK* |
