AlfalfaGEDB | MsG0680034468.01

M. sativa cultivar ZhongmuNo.1 / MsG0680034468.01

KEGG: -
GO: GO:0005515
PFAM: PF01535 PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034468.01.T01	XP_024641767.1	78.586	495	106	0	1	495	57	551	0	809
MsG0680034468.01.T01	XP_024641767.1	29.7	367	220	3	164	493	44	409	2.72E-43	174

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034468.01.T01	Q9SXD1	35.888	535	308	1	2	501	75	609	2.05E-121	376

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034468.01.T01	A0A396HN06	78.586	495	106	0	1	495	57	551	0.0	809

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680034468.01	PPI
MsG0080048802.01	MsG0680034468.01	PPI
MsG0080048802.01	MsG0680034468.01	PPI
MsG0080048925.01	MsG0680034468.01	PPI
MsG0080048925.01	MsG0680034468.01	PPI
MsG0080048926.01	MsG0680034468.01	PPI
MsG0180000086.01	MsG0680034468.01	PPI
MsG0180001046.01	MsG0680034468.01	PPI
MsG0180001047.01	MsG0680034468.01	PPI
MsG0180001883.01	MsG0680034468.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034468.01.T01	MTR_6g069300	78.296	493	107	0	1	493	57	549	0.0	804
MsG0680034468.01.T01	MTR_6g069300	29.211	380	269	0	115	494	66	445	9.23e-59	208
MsG0680034468.01.T01	MTR_6g069300	28.532	361	221	3	6	364	237	562	1.48e-39	154
MsG0680034468.01.T01	MTR_0110s0060	73.535	495	131	0	1	495	48	542	0.0	764
MsG0680034468.01.T01	MTR_0110s0060	29.668	391	233	4	164	518	35	419	4.34e-49	179
MsG0680034468.01.T01	MTR_6g079440	73.939	495	129	0	1	495	47	541	0.0	763
MsG0680034468.01.T01	MTR_6g079440	25.589	297	220	1	202	497	37	333	8.75e-29	121
MsG0680034468.01.T01	MTR_0308s0020	67.475	495	159	1	3	495	47	541	0.0	699
MsG0680034468.01.T01	MTR_6g068820	69.697	495	107	2	1	495	1	452	0.0	682
MsG0680034468.01.T01	MTR_0014s0210	65.859	495	164	2	3	495	5	496	0.0	678

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034468.01.T01	AT1G62670	35.888	535	308	1	2	501	75	609	2.09e-122	376
MsG0680034468.01.T01	AT1G62670	27.252	433	315	0	6	438	184	616	4.23e-67	231
MsG0680034468.01.T01	AT1G62670	29.723	397	277	1	114	510	82	476	1.23e-64	224
MsG0680034468.01.T01	AT1G62670	28.000	425	306	0	59	483	62	486	1.02e-63	222
MsG0680034468.01.T01	AT1G62670	29.825	342	203	3	166	507	64	368	7.99e-42	160
MsG0680034468.01.T01	AT1G62670	27.526	287	165	4	269	520	28	306	1.84e-22	102
MsG0680034468.01.T01	AT1G62670	35.888	535	308	1	2	501	75	609	2.09e-122	376
MsG0680034468.01.T01	AT1G62670	27.252	433	315	0	6	438	184	616	4.23e-67	231
MsG0680034468.01.T01	AT1G62670	29.723	397	277	1	114	510	82	476	1.23e-64	224
MsG0680034468.01.T01	AT1G62670	28.000	425	306	0	59	483	62	486	1.02e-63	222

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Find 126 sgRNAs with CRISPR-Local

Find 325 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTAACTTAAGAATCT+TGG	0.358085	6:-88048493	None:intergenic
AAAAGTGAAACCAAAAGAGC+CGG	0.559475	6:-88051388	None:intergenic
AAAATTGAGGTGACCAAGAT+GGG	0.495650	6:-88048454	None:intergenic
AAAGGGATTTCACCTTGACC+AGG	0.578719	6:+88048611	MsG0680034468.01.T01:CDS
AACATCATCATGAAAGTAAA+GGG	0.419733	6:-88048583	None:intergenic
AATAAGTGAGTTGTATGTTA+CGG	0.498778	6:-88049261	None:intergenic
AATCTTTGTAAGAACCTTCA+TGG	0.336899	6:-88048688	None:intergenic
AATGTCCTTATTGTCAGAAA+TGG	0.429894	6:+88049634	MsG0680034468.01.T01:CDS
AATTATGAATAGTTTATGCA+AGG	0.605408	6:+88048752	MsG0680034468.01.T01:CDS
AATTCAAATATGGGTGGGGT+AGG	0.481226	6:-88048303	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTCAGTTAACTTCTTCT+TGG	-	Chr6:88051456-88051475	None:intergenic	30.0%
AAGTAAGATAGGATACCATA+GGG	+	Chr6:88050733-88050752	MsG0680034468.01.T01:intron	30.0%
AATCTTTGTAAGAACCTTCA+TGG	-	Chr6:88048691-88048710	None:intergenic	30.0%
AATGTCCTTATTGTCAGAAA+TGG	+	Chr6:88049634-88049653	MsG0680034468.01.T01:CDS	30.0%
ACAATGACTACACTTATCAA+AGG	+	Chr6:88048531-88048550	MsG0680034468.01.T01:CDS	30.0%
ACATACAACTCACTTATTGA+TGG	+	Chr6:88049266-88049285	MsG0680034468.01.T01:CDS	30.0%
ACTTATACTTCTTTGATGGA+TGG	+	Chr6:88049056-88049075	MsG0680034468.01.T01:CDS	30.0%
ACTTTACAATTGGCAAGAAA+TGG	-	Chr6:88050288-88050307	None:intergenic	30.0%
AGCTACAGTGTTATGATTAA+CGG	+	Chr6:88049161-88049180	MsG0680034468.01.T01:CDS	30.0%
AGGGCTATAATTTAGATGTT+AGG	+	Chr6:88049558-88049577	MsG0680034468.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	88048294	88053215	88048294	ID=MsG0680034468.01;Name=MsG0680034468.01
Chr6	mRNA	88048294	88053215	88048294	ID=MsG0680034468.01.T01;Parent=MsG0680034468.01;Name=MsG0680034468.01.T01;_AED=0.39;_eAED=0.39;_QI=0\|0\|0\|0.2\|1\|1\|5\|0\|625
Chr6	exon	88048294	88049777	88048294	ID=MsG0680034468.01.T01:exon:18354;Parent=MsG0680034468.01.T01
Chr6	exon	88051363	88051415	88051363	ID=MsG0680034468.01.T01:exon:18355;Parent=MsG0680034468.01.T01
Chr6	exon	88052695	88052761	88052695	ID=MsG0680034468.01.T01:exon:18356;Parent=MsG0680034468.01.T01
Chr6	exon	88052819	88052923	88052819	ID=MsG0680034468.01.T01:exon:18357;Parent=MsG0680034468.01.T01
Chr6	exon	88053047	88053215	88053047	ID=MsG0680034468.01.T01:exon:18358;Parent=MsG0680034468.01.T01
Chr6	CDS	88048294	88049777	88048294	ID=MsG0680034468.01.T01:cds;Parent=MsG0680034468.01.T01
Chr6	CDS	88051363	88051415	88051363	ID=MsG0680034468.01.T01:cds;Parent=MsG0680034468.01.T01
Chr6	CDS	88052695	88052761	88052695	ID=MsG0680034468.01.T01:cds;Parent=MsG0680034468.01.T01
Chr6	CDS	88052819	88052923	88052819	ID=MsG0680034468.01.T01:cds;Parent=MsG0680034468.01.T01
Chr6	CDS	88053047	88053215	88053047	ID=MsG0680034468.01.T01:cds;Parent=MsG0680034468.01.T01

Gene Sequence

>MsG0680034468.01.T01

ATGAACAACCCTACCCCACCCATATTTGAATTTGGTAAGATCTTATCTTCTCTTGTTAAGTTGAACCATTTCCACACTGCTATTTCATTTTCTAAACAAATGGAACTCAAACAAATTCAACCTGACATTTTTACTTTAACCATCTTAATTAATTGTTACTCCCATCTTGGTCACCTCAATTTTGCTTTTTCTATGTTGGCCAAGATTCTTAAGTTAGGTTTTCAACCTGATACCATAACAATGACTACACTTATCAAAGGTCTGTGTCTTAATGGCAAGGTTAAGGAAGCCCTTTACTTTCATGATGATGTTAGAGCAAAGGGATTTCACCTTGACCAGGTTAGTTATGGGACCTTGATTGATGGGTTATGTAAAATAGGTGAAACAACAGCTGCCATGAAGGTTCTTACAAAGATTGATAAGTTGTTGGTTCAACCTGATGTCGTCATGTACAACACAATTATGAATAGTTTATGCAAGGATAAACTTGTAATTGATGCTTGTGATTTATATTCTCAAATGCTTCTCAAGAGAATTTCTCCTAATGTTATCACTTACAGTACTTTAATATATGGATTTTGTATTGTGGGTCAATTCAAACAAGCCATTGATATGTTAAATGATATGCTCGTGAAAAACATCAGCCCAAATGTTCGTACTTTTAACATATTGGTCGATGGTTTATGTAAGGAAGGAGAGGTGAAAAAAGCTAGAAATGTGTTAGCTGTTATGATAAAACAAGGTGTGGAACCTAATGTTGTTACTTATACTTCTTTGATGGATGGTTATTTCTTGGTTAAAGAAGTGAGCAGAGCCACACATGTATTCAACACTATGGCTCAAAGGGGAGTGACTCCTGATGTTCATAGCTACAGTGTTATGATTAACGGATTATGCAAGCATAAAATGGTGGATGAAGCTGTCAATATCTTCAAAGAAATGCAGTTAAAAAACATGGCTCCTAATACCGTAACATACAACTCACTTATTGATGGACTTTGCCAATTAGGGAAAATATCTGATGTTTTGGAACTTATAGATGAGATGCATGATAGAGGTCAACAAGCTAACATAATTACTTACAGTTCTTTACTAGATGCATTGTTCAAAAACCATCAGGTTGACAAGGCAATTGCATTATTGACGAAGATCAAAGACCAGGGAATTGAACCAGATATGTTCACATACACAATACTTGTTGATGGACTGTGCAAAAATGGAAGACTTAAGGATGCACAAAATGTATATCAGGATCTTCTGATTAAGGGCTATAATTTAGATGTTAGGATGTATAATGTGATGGTTAATGGGCTTTGTAAAGAGGGCTTGTTTGATGATGCAATGTCCTTATTGTCAGAAATGGAAGACAATGGTTGCACCCCTAATGCTGTAACTTTTCAAACTCTTATCCATGCTTTGTTTGAAAGCAACAAGAATGATAAGGCCGTGGAACTTCTACGTGAAATGATTGCTAGAGGTCTACTGTTCAACCCTTCGGTTGTTGTGATGCCGGCTCTTTTGGTTTCACTTTTGCTGGGCTTACTTCTGAATTACGGTCATTTATTGGGAGAACTAACCCCTACCCTATTCCCTCAATTAAAAATGTTTGATATTGAATTGTTATTACAAGTGGAGTACAGTAGGAAGAAATTCCCACTTCCATGGCATTTTAGCATATGTGAATTTACAAGATTTTGTCCTATTGGACTGTACCAAAAAGTTGATGAAAATGGAAGTTTCAGGTCATTCATGCTTTCATTTGACCTTGGACGTGATTGCAATAAGATGGGTCAAACTCTTTACTATATTTGCAAGATTTGGTCCTATTGGACATTTGGACTGTCTCATGTAATGCCTAAGTGTTATGGTGTTAATTAG

Protein sequence

>MsG0680034468.01.T01

MNNPTPPIFEFGKILSSLVKLNHFHTAISFSKQMELKQIQPDIFTLTILINCYSHLGHLNFAFSMLAKILKLGFQPDTITMTTLIKGLCLNGKVKEALYFHDDVRAKGFHLDQVSYGTLIDGLCKIGETTAAMKVLTKIDKLLVQPDVVMYNTIMNSLCKDKLVIDACDLYSQMLLKRISPNVITYSTLIYGFCIVGQFKQAIDMLNDMLVKNISPNVRTFNILVDGLCKEGEVKKARNVLAVMIKQGVEPNVVTYTSLMDGYFLVKEVSRATHVFNTMAQRGVTPDVHSYSVMINGLCKHKMVDEAVNIFKEMQLKNMAPNTVTYNSLIDGLCQLGKISDVLELIDEMHDRGQQANIITYSSLLDALFKNHQVDKAIALLTKIKDQGIEPDMFTYTILVDGLCKNGRLKDAQNVYQDLLIKGYNLDVRMYNVMVNGLCKEGLFDDAMSLLSEMEDNGCTPNAVTFQTLIHALFESNKNDKAVELLREMIARGLLFNPSVVVMPALLVSLLLGLLLNYGHLLGELTPTLFPQLKMFDIELLLQVEYSRKKFPLPWHFSICEFTRFCPIGLYQKVDENGSFRSFMLSFDLGRDCNKMGQTLYYICKIWSYWTFGLSHVMPKCYGVN*