AlfalfaGEDB | MsG0680033272.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033272.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033272.01.T01	XP_013443362.2	67.8	500	73	3	1	412	52	551	0	654

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033272.01.T01	P0C7Q7	34.632	462	247	3	1	409	79	538	2.96E-91	290

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033272.01.T01	A0A396HGV9	67.265	501	76	3	1	413	52	552	0.0	652

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680033272.01	PPI
MsG0080048802.01	MsG0680033272.01	PPI
MsG0080048925.01	MsG0680033272.01	PPI
MsG0180001046.01	MsG0680033272.01	PPI
MsG0180001047.01	MsG0680033272.01	PPI
MsG0180001883.01	MsG0680033272.01	PPI
MsG0180001883.01	MsG0680033272.01	PPI
MsG0180001883.01	MsG0680033272.01	PPI
MsG0180001883.01	MsG0680033272.01	PPI
MsG0180001883.01	MsG0680033272.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033272.01.T01	MTR_0019s0210	67.000	500	77	3	1	412	52	551	0.0	649
MsG0680033272.01.T01	MTR_0014s0250	66.733	499	77	3	1	411	48	545	0.0	645
MsG0680033272.01.T01	MTR_3g034990	65.932	499	82	2	1	411	57	555	0.0	641
MsG0680033272.01.T01	MTR_7g012890	65.130	499	86	2	1	411	48	546	0.0	635
MsG0680033272.01.T01	MTR_7g012890	25.210	238	124	3	210	410	45	265	7.69e-17	82.8
MsG0680033272.01.T01	MTR_0014s0180	63.928	499	90	3	1	411	52	548	0.0	617
MsG0680033272.01.T01	MTR_0014s0180	25.758	264	178	1	168	413	42	305	1.09e-25	109
MsG0680033272.01.T01	MTR_0429s0020	65.180	471	104	2	1	411	60	530	0.0	610
MsG0680033272.01.T01	MTR_0429s0020	28.384	229	144	2	202	410	49	277	4.45e-19	89.7
MsG0680033272.01.T01	MTR_0308s0020	62.725	499	98	2	1	411	43	541	0.0	606

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033272.01.T01	AT1G12700	34.632	462	247	3	1	409	79	538	3.02e-92	290
MsG0680033272.01.T01	AT1G12700	27.729	339	242	3	8	346	261	596	1.88e-44	163
MsG0680033272.01.T01	AT1G12700	29.344	259	165	1	170	410	71	329	2.05e-33	132
MsG0680033272.01.T01	AT1G12700	34.632	462	247	3	1	409	95	554	4.21e-92	290
MsG0680033272.01.T01	AT1G12700	27.729	339	242	3	8	346	277	612	2.09e-44	163
MsG0680033272.01.T01	AT1G12700	29.344	259	165	1	170	410	87	345	2.42e-33	132
MsG0680033272.01.T01	AT1G12700	34.632	462	247	3	1	409	95	554	4.21e-92	290
MsG0680033272.01.T01	AT1G12700	27.729	339	242	3	8	346	277	612	2.09e-44	163
MsG0680033272.01.T01	AT1G12700	29.344	259	165	1	170	410	87	345	2.42e-33	132
MsG0680033272.01.T01	AT3G22470	34.322	472	245	5	1	409	61	530	2.31e-91	287

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Find 71 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGTTGAGAAAGATTGAA+GGG	0.571677	6:+60785570	MsG0680033272.01.T01:CDS
AATAATAGTTTCATAAGTTA+CGG	0.451848	6:-60786557	None:intergenic
AATAGTTCTTAAAACATGTT+TGG	0.365220	6:-60785984	None:intergenic
AATCTTGTTAAATTCAATTA+TGG	0.199698	6:-60785183	None:intergenic
AATCTTTCTCAACATTTGCA+AGG	0.523976	6:-60785564	None:intergenic
AATTCAATTATGGATGGGGT+AGG	0.528128	6:-60785173	None:intergenic
ACAATCCATTTATCAAGGTC+TGG	0.465795	6:-60785517	None:intergenic
ACATAGATCATCAAGCAATA+TGG	0.410968	6:-60785834	None:intergenic
ACCTTGACTACTCTTGCCAA+AGG	0.544270	6:+60785407	MsG0680033272.01.T01:CDS
ACCTTTGGCAAGAGTAGTCA+AGG	0.535374	6:-60785408	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTTTCTCAACATTTGCA+AGG	-	Chr6:60785567-60785586	None:intergenic	30.0%
ACATAGATCATCAAGCAATA+TGG	-	Chr6:60785837-60785856	None:intergenic	30.0%
ACGTACAATTCATTAATGGA+TGG	+	Chr6:60785932-60785951	MsG0680033272.01.T01:CDS	30.0%
ATGACAACTTAAGAATGCAT+GGG	+	Chr6:60786387-60786406	MsG0680033272.01.T01:intron	30.0%
CAAATGTTGAGAAAGATTGA+AGG	+	Chr6:60785569-60785588	MsG0680033272.01.T01:CDS	30.0%
CCTAGTTAATGAAGTGAATA+AGG	+	Chr6:60785961-60785980	MsG0680033272.01.T01:CDS	30.0%
CCTTATTCACTTCATTAACT+AGG	-	Chr6:60785964-60785983	None:intergenic	30.0%
GAATTATAAGTAACCGTATC+AGG	-	Chr6:60786133-60786152	None:intergenic	30.0%
GAATTGTACGTAACAATATC+TGG	-	Chr6:60785923-60785942	None:intergenic	30.0%
GTGAAGAAAATTAAAGACCA+AGG	+	Chr6:60786310-60786329	MsG0680033272.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	60785158	60786663	60785158	ID=MsG0680033272.01;Name=MsG0680033272.01
Chr6	mRNA	60785158	60786663	60785158	ID=MsG0680033272.01.T01;Parent=MsG0680033272.01;Name=MsG0680033272.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|1\|1\|1\|3\|0\|413
Chr6	exon	60785158	60786014	60785158	ID=MsG0680033272.01.T01:exon:4061;Parent=MsG0680033272.01.T01
Chr6	exon	60786120	60786331	60786120	ID=MsG0680033272.01.T01:exon:4060;Parent=MsG0680033272.01.T01
Chr6	exon	60786491	60786663	60786491	ID=MsG0680033272.01.T01:exon:4059;Parent=MsG0680033272.01.T01
Chr6	CDS	60785158	60786014	60785158	ID=MsG0680033272.01.T01:cds;Parent=MsG0680033272.01.T01
Chr6	CDS	60786120	60786331	60786120	ID=MsG0680033272.01.T01:cds;Parent=MsG0680033272.01.T01
Chr6	CDS	60786491	60786663	60786491	ID=MsG0680033272.01.T01:cds;Parent=MsG0680033272.01.T01

Gene Sequence

>MsG0680033272.01.T01

ATGCTTCGTATGAATCCTACCCCATCCATAATTGAATTTAACAAGATTTTAGGTTCCCTTGTTAAGTCTAACAATAAACATTACCCAACTGCTATTTCCCTTTCTCATCGATTGGAATTCCATGGAATTATTCCTAATATTATTACTTTCAACATCCTGATCAACTGTTTCTGTCATCTTGGTCAAATGAATTTTCCCTTTTCTATTTTGGCGAAAATTCTCAAGATGGGTTATCATCCCAATACCATAACCTTGACTACTCTTGCCAAAGGTTTTTGTTTAAACCATAAGGTCAAGGAAGCTCTACACTTTCACGACCACGTGCTTGCACTTGGATTTCACTTAGACCAAGTTAGCTACCAGACCTTGATAAATGGATTGTGTAAAATGGGAGAAACAATAGCTGCCTTGCAAATGTTGAGAAAGATTGAAGGGAAATTGGTCAACACCAATGTGGTAATGTATAGCATAATTATTGATAGTTTGTGTAAAGATAAACTTGTAATTGATGCTTATGAGTTATATTCTGAAATGATTGCAAAGAAAATTTCTCCTGATGTTGTCACTTTCAATTCTCTAATATATGAATTTTGCATTGTTGGTCAATTGAAAGATGCATTTGGTTTGTTCCATCAAATGGTATTGAAAAACATTAACCCGAGCGTGTATACTTTTACCATATTGCTTGATGATCTATGTAAGGAAGGAAAGATGAAAGAGGCTAAAAATGTGTTGGCTATGATGATGAAAGGAAGTATAAAACCAGATATTGTTACGTACAATTCATTAATGGATGGGTATTGCCTAGTTAATGAAGTGAATAAGGCCAAACATGTTTTAAGAACTATTGCTCGAATAGGAATTGCTCCTGATACGGTTACTTATAATTCTCTCATTGATGGTTTGTGCAAATCAGGTAGAATCTCCTATGCTTGGGAGCTTGTTGATGAGATGCATGTTAATTGTCAACCACCTGACATATTCACTTACAGTTCTTTAATAGATTCTTTATGCAAAAGCAATCATCTTGACAAGGCTATTGCATTAGTGAAGAAAATTAAAGACCAAGAGGGCTTGTTTGATGAAGCAGAGGAGTTGTTGTCAAAAATGAATGACAATGGTATCATTCCTGATGCCGTAACTTATGAAACTATTATTCGCGCACTCTTTCATAAAGACGAGAATGAAAAAGCGGAGAAACTCCTTCGAAAAATGATTGCTAGAGGCCTACTAAAAGAGTAA

Protein sequence

>MsG0680033272.01.T01

MLRMNPTPSIIEFNKILGSLVKSNNKHYPTAISLSHRLEFHGIIPNIITFNILINCFCHLGQMNFPFSILAKILKMGYHPNTITLTTLAKGFCLNHKVKEALHFHDHVLALGFHLDQVSYQTLINGLCKMGETIAALQMLRKIEGKLVNTNVVMYSIIIDSLCKDKLVIDAYELYSEMIAKKISPDVVTFNSLIYEFCIVGQLKDAFGLFHQMVLKNINPSVYTFTILLDDLCKEGKMKEAKNVLAMMMKGSIKPDIVTYNSLMDGYCLVNEVNKAKHVLRTIARIGIAPDTVTYNSLIDGLCKSGRISYAWELVDEMHVNCQPPDIFTYSSLIDSLCKSNHLDKAIALVKKIKDQEGLFDEAEELLSKMNDNGIIPDAVTYETIIRALFHKDENEKAEKLLRKMIARGLLKE*