AlfalfaGEDB | MsG0680033276.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033276.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033276.01.T01	KEH17452.1	71.459	466	103	1	1	436	51	516	0	676
MsG0680033276.01.T01	KEH17452.1	29.462	353	207	4	43	359	198	544	2.58E-43	174
MsG0680033276.01.T01	KEH17452.1	27.65	217	151	3	4	220	335	545	9.05E-13	83.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033276.01.T01	Q3ECK2	39.387	424	251	2	2	425	80	497	1.10E-103	327

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033276.01.T01	A0A072TJ47	71.459	466	103	1	1	436	51	516	0.0	676

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680033276.01	PPI
MsG0080048802.01	MsG0680033276.01	PPI
MsG0080048802.01	MsG0680033276.01	PPI
MsG0080048849.01	MsG0680033276.01	PPI
MsG0080048925.01	MsG0680033276.01	PPI
MsG0080048925.01	MsG0680033276.01	PPI
MsG0080048926.01	MsG0680033276.01	PPI
MsG0180000086.01	MsG0680033276.01	PPI
MsG0180001046.01	MsG0680033276.01	PPI
MsG0180001047.01	MsG0680033276.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033276.01.T01	MTR_0014s0250	71.459	466	103	1	1	436	51	516	0.0	676
MsG0680033276.01.T01	MTR_0014s0250	29.462	353	207	4	43	359	198	544	3.13e-47	174
MsG0680033276.01.T01	MTR_0014s0250	27.650	217	151	3	4	220	335	545	1.10e-16	83.6
MsG0680033276.01.T01	MTR_0014s0180	69.762	463	109	2	4	436	58	519	0.0	652
MsG0680033276.01.T01	MTR_0014s0180	29.661	354	243	2	6	359	200	547	9.74e-46	170
MsG0680033276.01.T01	MTR_0014s0180	23.963	217	159	2	4	220	338	548	4.87e-14	75.5
MsG0680033276.01.T01	MTR_0014s0180	26.380	163	120	0	274	436	42	204	1.88e-11	67.0
MsG0680033276.01.T01	MTR_0019s0210	70.021	467	109	2	1	436	55	521	0.0	650
MsG0680033276.01.T01	MTR_0019s0210	30.247	324	215	3	39	359	234	549	9.59e-42	159
MsG0680033276.01.T01	MTR_0019s0210	29.032	310	217	2	134	441	42	350	1.13e-41	158

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033276.01.T01	AT1G62680	39.387	424	251	2	2	425	80	497	1.12e-104	327
MsG0680033276.01.T01	AT1G62680	33.228	316	211	0	130	445	62	377	1.96e-50	183
MsG0680033276.01.T01	AT1G62680	39.387	424	251	2	2	425	80	497	1.12e-104	327
MsG0680033276.01.T01	AT1G62680	33.228	316	211	0	130	445	62	377	1.96e-50	183
MsG0680033276.01.T01	AT1G12700	35.792	461	265	3	2	432	83	542	3.22e-102	322
MsG0680033276.01.T01	AT1G12700	29.799	349	240	2	25	373	245	588	4.14e-50	183
MsG0680033276.01.T01	AT1G12700	30.897	301	208	0	136	436	71	371	3.23e-47	175
MsG0680033276.01.T01	AT1G12700	26.543	162	119	0	275	436	70	231	2.63e-12	70.1
MsG0680033276.01.T01	AT1G12700	35.792	461	265	3	2	432	99	558	3.94e-102	322
MsG0680033276.01.T01	AT1G12700	29.799	349	240	2	25	373	261	604	4.74e-50	183

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Find 119 sgRNAs with CRISPR-Local

Find 441 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAATCGAAGGGAAATT+GGG	0.454722	6:+60852504	MsG0680033276.01.T01:intron
AAATTACCAATGGTTCCCTA+TGG	0.520787	6:+60853777	MsG0680033276.01.T01:CDS
AACGGGGAAGGCTCCGTAAG+GGG	0.460124	6:+60855810	MsG0680033276.01.T01:CDS
AAGCATTATTTCTCGAATAG+GGG	0.598350	6:+60852915	MsG0680033276.01.T01:CDS
AAGGCTACAATGTATCAGTC+TGG	0.495670	6:+60853352	MsG0680033276.01.T01:CDS
AATAATGCTTAGCACATGTT+TGG	0.411816	6:-60852902	None:intergenic
AATCCATAGCATACTGGTTT+TGG	0.273984	6:+60857638	MsG0680033276.01.T01:CDS
AATTGCCTTGTCAACATAGT+GGG	0.512191	6:-60853199	None:intergenic
ACAGAGCCTCGACTTCATCA+AGG	0.606861	6:-60853416	None:intergenic
ACAGTTTAAACTATGTCAGT+AGG	0.591561	6:-60857711	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAATCGAAGGGAAATT+GGG	+	Chr6:60852504-60852523	MsG0680033276.01.T01:intron	30.0%
AAATACTCCAAAGACAGATA+AGG	-	Chr6:60857576-60857595	None:intergenic	30.0%
AAGAGTTAATTAGCACTCAT+TGG	-	Chr6:60857075-60857094	None:intergenic	30.0%
AATAATGCTTAGCACATGTT+TGG	-	Chr6:60852905-60852924	None:intergenic	30.0%
AATGTCCATTGAAAACAAGT+TGG	-	Chr6:60857479-60857498	None:intergenic	30.0%
ACATACACTACACTTATTGA+TGG	+	Chr6:60853270-60853289	MsG0680033276.01.T01:CDS	30.0%
ACGTGGAAAGGATATTTAAT+TGG	+	Chr6:60854130-60854149	MsG0680033276.01.T01:intron	30.0%
ACTTACAATTCTCTCATTGA+TGG	+	Chr6:60853060-60853079	MsG0680033276.01.T01:CDS	30.0%
ACTTTCAGTTCTCTAATCTA+TGG	+	Chr6:60852640-60852659	MsG0680033276.01.T01:CDS	30.0%
AGAAAGAGCAATAAATGGAT+TGG	+	Chr6:60854746-60854765	MsG0680033276.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	60852096	60857728	60852096	ID=MsG0680033276.01;Name=MsG0680033276.01
Chr6	mRNA	60852096	60857728	60852096	ID=MsG0680033276.01.T01;Parent=MsG0680033276.01;Name=MsG0680033276.01.T01;_AED=0.51;_eAED=0.51;_QI=0\|0\|0\|0.28\|1\|1\|7\|0\|587
Chr6	exon	60852096	60852434	60852096	ID=MsG0680033276.01.T01:exon:4042;Parent=MsG0680033276.01.T01
Chr6	exon	60852517	60853476	60852517	ID=MsG0680033276.01.T01:exon:4041;Parent=MsG0680033276.01.T01
Chr6	exon	60853751	60853838	60853751	ID=MsG0680033276.01.T01:exon:4040;Parent=MsG0680033276.01.T01
Chr6	exon	60855754	60855840	60855754	ID=MsG0680033276.01.T01:exon:4039;Parent=MsG0680033276.01.T01
Chr6	exon	60856570	60856671	60856570	ID=MsG0680033276.01.T01:exon:4038;Parent=MsG0680033276.01.T01
Chr6	exon	60856773	60856859	60856773	ID=MsG0680033276.01.T01:exon:4037;Parent=MsG0680033276.01.T01
Chr6	exon	60857628	60857728	60857628	ID=MsG0680033276.01.T01:exon:4036;Parent=MsG0680033276.01.T01
Chr6	CDS	60852096	60852434	60852096	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60852517	60853476	60852517	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60853751	60853838	60853751	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60855754	60855840	60855754	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60856570	60856671	60856570	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60856773	60856859	60856773	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01
Chr6	CDS	60857628	60857728	60857628	ID=MsG0680033276.01.T01:cds;Parent=MsG0680033276.01.T01

Gene Sequence

>MsG0680033276.01.T01

ATGCGTCATACTCCATCCATTATTGAATTTAACAAGATCTTAACTTACCTTGTTAAAACTAAGACTCACTACAACACTGTTATTTCCTTTTTCCGACTAATGCAATATAACGGAATTGTGCCAACTATTGTTACTCTCGGCATCGTCATCAATTGTTACTGCCATCTACGTGAAATCAATTTTGCATTTTCTATGTTGGCAAAGATTCTTAAGATGGGTTATAAGCCAGATACCATAACCTTGACTACACTTATGAAAGGTTTGTGTTTTAATGGTAAGGTCGAGGAAGCTCTCCACTTTCACAACCATGTTCTTGCACTTGGATTTCACTTGGATCATGGAAATTGGGCAACACCAATTTATAACACAATCATTGATAGTTTGTGTAAAGATAAACTTGTAACCAATGCCTATGAGTTATACTCTAAAATGATTGCAAAGAGAATTTCTCCTACTGTTGTCACTTTCAGTTCTCTAATCTATGGATTTTGTATTGTTGGTCAATTCAAAGATGCATTTCATTTGTTCCATGAAATGGTATTGAAAAACATCAACCCAAATGTTTATACTTTTAATATTTTGGTTGATGCTCTTTGTAAGGAAGGAGATGTGAAAGGAGCTAAAAGTTTCTTAGTCGTGATGATGAAAGAAGGGGTAATACCAGATGTTGTTACATACAATTCATTAATGGATGGATATTGCCTTGTTAATGAAGTCAATAAGGCCAAACATGTGCTAAGCATTATTTCTCGAATAGGGGTGGCTCCTGATGCTCATAGTTATAGTATCATGATTAATGGATTCTGTAAGATTAAAATGGTCGACGAAGCCTTGAGTCTCTTCGATGAAATGTGTTGCAGAGGAATTGCTCCTAATACGGTAACTTACAATTCTCTCATTGATGGTTTGTGCAAATCTGGGAGAGTTTCTAATGCTTGGAAGCTACTTGATCAGATGCATGACAGAGGTCAACCTTCCGATATAATTACTTACAATTCCTTATTACATGCTTTATGTAAAACCCACTATGTTGACAAGGCAATTGAATTGGTCAAAAATATTAAAGACCAAGGCATTCAACCTGATATGCACACATACACTACACTTATTGATGGATTATGCAAACAAGGAAGACTTAAGGACGCACAAGTGATTTTTCAGGATCTTTTGATTAAAGGCTACAATGTATCAGTCTGGACATATAATATTATGATCAATGGTCTTTGTTTAGAGGGCTTCCTTGATGAAGTCGAGGCTCTGTTGTCAAAAATGGAAGACAACTTATGCATTCCTGATGTTGTTCATTTTTATATTTTCATGATACCAAATTACCAATGGTTCCCTATGGCTTTCAGAGTTTTGAATTTAAGTAAAGCTAGAGTTGTGAGTGCCATGTCTCTTGCAATCCATGAAGGTATGGATTCAAGGGGTTCTCATGATGGGAACGGGGAAGGCTCCGTAAGGGGATCACATGATAAGCAGGATGTTTATGCATGTTCGGTGCCGGATGGATATTACGTCAAGGGTTCGTTCGTAAAGCTGCGGGACGATATGGACATCAAGGATGTATTTTTATTACTAATATTAACGCACGTGCCAGACTTTCTAGATACGTCTGGTATGGGTGTTACAGATAGAACTCGTAAAATCAAGATACAAAACCGGTGTGTGGTAATCCATAGCATACTGGTTTTGGATGGCTTGAGGATAAAGAAAGTACAGGATATTGATGTTTACTGTTTCTTGCCTACTGACATAGTTTAA

Protein sequence

>MsG0680033276.01.T01

MRHTPSIIEFNKILTYLVKTKTHYNTVISFFRLMQYNGIVPTIVTLGIVINCYCHLREINFAFSMLAKILKMGYKPDTITLTTLMKGLCFNGKVEEALHFHNHVLALGFHLDHGNWATPIYNTIIDSLCKDKLVTNAYELYSKMIAKRISPTVVTFSSLIYGFCIVGQFKDAFHLFHEMVLKNINPNVYTFNILVDALCKEGDVKGAKSFLVVMMKEGVIPDVVTYNSLMDGYCLVNEVNKAKHVLSIISRIGVAPDAHSYSIMINGFCKIKMVDEALSLFDEMCCRGIAPNTVTYNSLIDGLCKSGRVSNAWKLLDQMHDRGQPSDIITYNSLLHALCKTHYVDKAIELVKNIKDQGIQPDMHTYTTLIDGLCKQGRLKDAQVIFQDLLIKGYNVSVWTYNIMINGLCLEGFLDEVEALLSKMEDNLCIPDVVHFYIFMIPNYQWFPMAFRVLNLSKARVVSAMSLAIHEGMDSRGSHDGNGEGSVRGSHDKQDVYACSVPDGYYVKGSFVKLRDDMDIKDVFLLLILTHVPDFLDTSGMGVTDRTRKIKIQNRCVVIHSILVLDGLRIKKVQDIDVYCFLPTDIV*