AlfalfaGEDB | MsG0880045148.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045148.01

KEGG: -
GO: GO:0003676
PFAM: PF00076

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045148.01.T01	KEH20067.1	87.458	295	10	1	1	268	1	295	2.69E-177	500

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045148.01.T01	Q9LFD5	60	230	67	4	18	225	4	230	2.81E-87	263

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045148.01.T01	A0A072TRN4	87.458	295	10	1	1	268	1	295	1.29e-177	500

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048202.01	MsG0880045148.01	0.802576	5.253543e-49	2.582465e-46
MsG0080048312.01	MsG0880045148.01	0.825088	5.672114e-54	5.119087e-51
MsG0080048345.01	MsG0880045148.01	0.808288	3.334186e-50	1.898953e-47
MsG0080048347.01	MsG0880045148.01	0.828561	8.393891e-55	8.373267e-52
MsG0080048352.01	MsG0880045148.01	0.820845	5.530992e-53	4.426259e-50
MsG0080048889.01	MsG0880045148.01	0.801978	6.976021e-49	3.377196e-46
MsG0080048919.01	MsG0880045148.01	-0.814835	1.258571e-51	8.535489e-49
MsG0080049066.01	MsG0880045148.01	-0.838405	2.931957e-57	3.923100e-54
MsG0080049133.01	MsG0880045148.01	-0.805168	1.520335e-49	7.987873e-47
MsG0180000583.01	MsG0880045148.01	0.821156	4.690265e-53	3.786034e-50
MsG0180000763.01	MsG0880045148.01	0.805789	1.126505e-49	6.013999e-47
MsG0180000930.01	MsG0880045148.01	0.800133	1.662933e-48	7.683316e-46
MsG0180001484.01	MsG0880045148.01	-0.801833	7.470695e-49	3.603620e-46
MsG0180002099.01	MsG0880045148.01	-0.806487	8.032561e-50	4.365287e-47
MsG0880044342.01	MsG0880045148.01	0.838617	2.585529e-57	3.482084e-54
MsG0880045148.01	MsG0880045270.01	0.807126	5.887225e-50	3.252367e-47
MsG0880045148.01	MsG0880045369.01	-0.809668	1.688447e-50	9.974562e-48
MsG0880045148.01	MsG0880045385.01	-0.815342	9.709418e-52	6.676663e-49
MsG0880045148.01	MsG0880045413.01	-0.812507	4.094503e-51	2.608196e-48
MsG0880045148.01	MsG0880045458.01	0.822553	2.227187e-53	1.870446e-50
MsG0880045148.01	MsG0880045968.01	-0.832272	1.039243e-55	1.155715e-52
MsG0880045148.01	MsG0880046010.01	-0.838934	2.140372e-57	2.910487e-54
MsG0880045148.01	MsG0880046219.01	-0.803175	3.952512e-49	1.972853e-46
MsG0880045148.01	MsG0880046341.01	-0.831779	1.375598e-55	1.508191e-52
MsG0880045148.01	MsG0880046429.01	0.804486	2.111216e-49	1.089861e-46
MsG0880045148.01	MsG0880046469.01	-0.804323	2.282468e-49	1.173368e-46
MsG0880045148.01	MsG0880046863.01	0.803750	3.003136e-49	1.521297e-46
MsG0180003405.01	MsG0880045148.01	-0.801158	1.027628e-48	4.873162e-46
MsG0180003757.01	MsG0880045148.01	-0.822796	1.955886e-53	1.653783e-50
MsG0180003758.01	MsG0880045148.01	-0.827729	1.332366e-54	1.297161e-51
MsG0180004581.01	MsG0880045148.01	-0.814435	1.542666e-51	1.035071e-48
MsG0180004773.01	MsG0880045148.01	-0.820505	6.621451e-53	5.248763e-50
MsG0180005272.01	MsG0880045148.01	0.829729	4.373645e-55	4.514983e-52
MsG0180005328.01	MsG0880045148.01	0.802775	4.780126e-49	2.361845e-46
MsG0180005356.01	MsG0880045148.01	0.813069	3.085214e-51	1.994549e-48
MsG0180005474.01	MsG0880045148.01	-0.819735	9.934097e-53	7.708317e-50
MsG0180005848.01	MsG0880045148.01	-0.822298	2.552680e-53	2.128149e-50
MsG0180006227.01	MsG0880045148.01	-0.819425	1.169129e-52	8.993684e-50
MsG0280006381.01	MsG0880045148.01	-0.813605	2.351531e-51	1.542654e-48
MsG0780040685.01	MsG0880045148.01	-0.800113	1.678427e-48	7.750838e-46
MsG0780041198.01	MsG0880045148.01	0.817916	2.573098e-52	1.898574e-49
MsG0780041556.01	MsG0880045148.01	0.811910	5.525889e-51	3.463488e-48
MsG0780041620.01	MsG0880045148.01	-0.828315	9.627969e-55	9.535070e-52
MsG0780041663.01	MsG0880045148.01	0.830205	3.347982e-55	3.503907e-52
MsG0780041720.01	MsG0880045148.01	-0.814075	1.852846e-51	1.231090e-48
MsG0780041783.01	MsG0880045148.01	-0.813274	2.781205e-51	1.808106e-48
MsG0880041835.01	MsG0880045148.01	-0.800717	1.264057e-48	5.927435e-46
MsG0880041855.01	MsG0880045148.01	-0.809648	1.705368e-50	1.006926e-47
MsG0880042495.01	MsG0880045148.01	0.818873	1.561741e-52	1.183193e-49
MsG0880042740.01	MsG0880045148.01	0.804176	2.449783e-49	1.254614e-46
MsG0880042858.01	MsG0880045148.01	-0.826703	2.346024e-54	2.216781e-51
MsG0280011165.01	MsG0880045148.01	0.827243	1.742248e-54	1.672503e-51
MsG0280011193.01	MsG0880045148.01	0.822783	1.969310e-53	1.664598e-50
MsG0280011203.01	MsG0880045148.01	0.817498	3.196961e-52	2.332088e-49
MsG0280011281.01	MsG0880045148.01	-0.834994	2.172598e-56	2.621550e-53
MsG0280011301.01	MsG0880045148.01	-0.815728	7.968912e-52	5.538349e-49
MsG0380012148.01	MsG0880045148.01	-0.802279	6.049144e-49	2.951237e-46
MsG0380013114.01	MsG0880045148.01	-0.825511	4.504413e-54	4.114045e-51
MsG0380013272.01	MsG0880045148.01	0.806301	8.793282e-50	4.756010e-47
MsG0380014639.01	MsG0880045148.01	-0.832088	1.153645e-55	1.276148e-52
MsG0380014642.01	MsG0880045148.01	-0.822234	2.642247e-53	2.198695e-50
MsG0380014735.01	MsG0880045148.01	-0.819169	1.337626e-52	1.021643e-49
MsG0680031240.01	MsG0880045148.01	0.801301	9.607035e-49	4.572129e-46
MsG0680031449.01	MsG0880045148.01	-0.801091	1.060141e-48	5.019110e-46
MsG0680031813.01	MsG0880045148.01	-0.807837	4.158479e-50	2.340375e-47
MsG0680034229.01	MsG0880045148.01	-0.813098	3.039897e-51	1.966729e-48
MsG0680035584.01	MsG0880045148.01	-0.844476	7.384340e-59	1.193549e-55
MsG0480018463.01	MsG0880045148.01	-0.800770	1.233025e-48	5.789657e-46
MsG0480018563.01	MsG0880045148.01	0.815507	8.922251e-52	6.163386e-49
MsG0480018628.01	MsG0880045148.01	-0.818006	2.455052e-52	1.815949e-49
MsG0480019183.01	MsG0880045148.01	-0.808393	3.167044e-50	1.808745e-47
MsG0480020842.01	MsG0880045148.01	-0.815216	1.035588e-51	7.095957e-49
MsG0480021131.01	MsG0880045148.01	0.821461	3.988222e-53	3.246627e-50
MsG0480021327.01	MsG0880045148.01	-0.804938	1.698838e-49	8.873551e-47
MsG0480022515.01	MsG0880045148.01	0.803225	3.858754e-49	1.928485e-46
MsG0480022655.01	MsG0880045148.01	0.838961	2.106527e-57	2.866701e-54
MsG0480022812.01	MsG0880045148.01	0.827890	1.218320e-54	1.191759e-51
MsG0480023062.01	MsG0880045148.01	0.823619	1.256201e-53	1.087225e-50
MsG0480023067.01	MsG0880045148.01	0.831033	2.099897e-55	2.251403e-52
MsG0480023152.01	MsG0880045148.01	-0.823150	1.617515e-53	1.381489e-50
MsG0480023244.01	MsG0880045148.01	-0.804288	2.321399e-49	1.192303e-46
MsG0480023695.01	MsG0880045148.01	-0.833335	5.658496e-56	6.496921e-53
MsG0580024398.01	MsG0880045148.01	0.820100	8.197454e-53	6.426559e-50
MsG0580024490.01	MsG0880045148.01	0.822100	2.837034e-53	2.351695e-50
MsG0580024493.01	MsG0880045148.01	-0.809137	2.195443e-50	1.278744e-47
MsG0580025105.01	MsG0880045148.01	-0.803410	3.532688e-49	1.773952e-46
MsG0580025486.01	MsG0880045148.01	-0.802750	4.837189e-49	2.388493e-46
MsG0580025746.01	MsG0880045148.01	0.832726	8.017114e-56	9.038655e-53
MsG0380015201.01	MsG0880045148.01	-0.803707	3.066021e-49	1.551367e-46
MsG0380015369.01	MsG0880045148.01	0.831453	1.655305e-55	1.797147e-52
MsG0380015616.01	MsG0880045148.01	0.827312	1.677052e-54	1.613172e-51
MsG0380015752.01	MsG0880045148.01	-0.800714	1.266303e-48	5.937335e-46
MsG0380016801.01	MsG0880045148.01	0.834771	2.472025e-56	2.962706e-53
MsG0380016835.01	MsG0880045148.01	-0.805062	1.600345e-49	8.385347e-47
MsG0380016839.01	MsG0880045148.01	0.800549	1.368310e-48	6.389046e-46
MsG0380016931.01	MsG0880045148.01	-0.801793	7.614402e-49	3.669113e-46
MsG0380016934.01	MsG0880045148.01	-0.817136	3.856765e-52	2.785559e-49
MsG0380016935.01	MsG0880045148.01	-0.815040	1.133293e-51	7.728848e-49
MsG0380017405.01	MsG0880045148.01	-0.805332	1.404917e-49	7.412399e-47
MsG0380017812.01	MsG0880045148.01	-0.820400	6.997432e-53	5.531190e-50
MsG0380017928.01	MsG0880045148.01	-0.815176	1.057159e-51	7.235854e-49
MsG0380017962.01	MsG0880045148.01	-0.800452	1.432014e-48	6.670054e-46
MsG0380018051.01	MsG0880045148.01	0.811634	6.346291e-51	3.948528e-48
MsG0480018201.01	MsG0880045148.01	-0.825597	4.298784e-54	3.936073e-51
MsG0480018202.01	MsG0880045148.01	-0.842324	2.772924e-58	4.189403e-55
MsG0480018203.01	MsG0880045148.01	-0.833907	4.072968e-56	4.757497e-53
MsG0580026095.01	MsG0880045148.01	0.832787	7.744754e-56	8.746909e-53
MsG0580026529.01	MsG0880045148.01	0.813716	2.223086e-51	1.462807e-48
MsG0580027638.01	MsG0880045148.01	0.802756	4.822780e-49	2.381713e-46
MsG0580028999.01	MsG0880045148.01	0.809770	1.605489e-50	9.510311e-48
MsG0580029296.01	MsG0880045148.01	0.803816	2.909719e-49	1.476521e-46
MsG0580029782.01	MsG0880045148.01	0.809873	1.525729e-50	9.061823e-48
MsG0580030133.01	MsG0880045148.01	0.817102	3.924723e-52	2.831927e-49
MsG0580030197.01	MsG0880045148.01	-0.800646	1.307408e-48	6.119819e-46
MsG0680030635.01	MsG0880045148.01	-0.800874	1.174593e-48	5.530141e-46
MsG0280006883.01	MsG0880045148.01	0.829236	5.763557e-55	5.864411e-52
MsG0280007216.01	MsG0880045148.01	0.855131	7.749899e-62	1.770867e-58
MsG0280008405.01	MsG0880045148.01	0.811625	6.376303e-51	3.966341e-48
MsG0280008719.01	MsG0880045148.01	0.818565	1.834265e-52	1.377808e-49
MsG0280009079.01	MsG0880045148.01	-0.807263	5.504606e-50	3.052167e-47
MsG0280009094.01	MsG0880045148.01	-0.809860	1.535377e-50	9.115965e-48
MsG0280009167.01	MsG0880045148.01	0.812586	3.934641e-51	2.511523e-48
MsG0280009742.01	MsG0880045148.01	-0.805007	1.643117e-49	8.597202e-47
MsG0280010149.01	MsG0880045148.01	-0.803983	2.686872e-49	1.369317e-46
MsG0280010240.01	MsG0880045148.01	0.815729	7.963560e-52	5.534856e-49
MsG0780035993.01	MsG0880045148.01	0.827501	1.510945e-54	1.461337e-51
MsG0780036661.01	MsG0880045148.01	0.841822	3.762899e-58	5.595560e-55
MsG0780037458.01	MsG0880045148.01	-0.801560	8.500353e-49	4.071893e-46
MsG0780039287.01	MsG0880045148.01	-0.827100	1.884824e-54	1.801691e-51
MsG0780039821.01	MsG0880045148.01	-0.801331	9.471110e-49	4.510826e-46
MsG0780039836.01	MsG0880045148.01	-0.807270	5.487544e-50	3.043220e-47
MsG0780039838.01	MsG0880045148.01	-0.808647	2.794773e-50	1.606646e-47
MsG0780039839.01	MsG0880045148.01	-0.830298	3.177021e-55	3.333569e-52
MsG0780040181.01	MsG0880045148.01	0.808437	3.097972e-50	1.771400e-47

PPI

Gene1	Gene2	Type
MsG0780040190.01	MsG0880045148.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045148.01.T01	MTR_8g066460	87.458	295	10	1	1	268	1	295	0.0	500
MsG0880045148.01.T01	MTR_8g066460	87.097	279	9	1	17	268	1	279	3.44e-170	471
MsG0880045148.01.T01	MTR_5g029640	70.866	254	47	1	17	243	1	254	2.15e-118	347
MsG0880045148.01.T01	MTR_5g029640	76.923	156	36	0	96	251	279	434	1.19e-72	230
MsG0880045148.01.T01	MTR_1g032860	51.969	254	91	3	19	242	31	283	2.77e-84	254
MsG0880045148.01.T01	MTR_8g105910	49.097	277	102	4	12	261	26	290	8.16e-82	248
MsG0880045148.01.T01	MTR_7g086080	44.980	249	94	2	17	223	1	248	4.11e-65	204
MsG0880045148.01.T01	MTR_5g039130	33.750	240	113	2	22	219	10	245	4.26e-38	134
MsG0880045148.01.T01	MTR_7g010100	30.196	255	111	5	21	218	7	251	1.00e-26	104
MsG0880045148.01.T01	MTR_5g038107	66.129	62	21	0	17	78	1	62	1.15e-22	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045148.01.T01	AT4G17720	61.567	268	73	2	17	254	1	268	3.95e-106	310
MsG0880045148.01.T01	AT5G46870	54.643	280	91	3	17	260	1	280	9.76e-91	271
MsG0880045148.01.T01	AT5G46870	54.643	280	91	3	17	260	1	280	9.76e-91	271
MsG0880045148.01.T01	AT5G16840	60.000	230	68	3	18	225	4	231	9.14e-90	267
MsG0880045148.01.T01	AT5G16840	60.000	230	67	4	18	225	4	230	2.87e-88	263
MsG0880045148.01.T01	AT5G16840	59.307	231	69	4	17	225	1	228	1.25e-87	261
MsG0880045148.01.T01	AT1G67950	48.485	231	89	4	19	226	29	252	4.81e-66	207
MsG0880045148.01.T01	AT1G67950	48.485	231	89	4	19	226	28	251	5.09e-66	207
MsG0880045148.01.T01	AT1G67950	48.276	232	89	4	19	226	28	252	6.74e-66	207
MsG0880045148.01.T01	AT5G32450	44.715	246	94	2	20	223	5	250	5.32e-63	199
MsG0880045148.01.T01	AT1G67950	44.724	199	80	4	51	226	1	192	1.32e-47	158
MsG0880045148.01.T01	AT1G14340	32.203	236	118	2	22	219	9	240	6.31e-34	123
MsG0880045148.01.T01	AT3G01210	28.395	243	119	4	23	217	9	244	1.98e-21	90.5
MsG0880045148.01.T01	AT3G01210	28.395	243	119	4	23	217	9	244	1.98e-21	90.5

Find 67 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTTGTTGCTGCGGCTTT+TGG	0.121118	8:-54932415	None:intergenic
CTTGCGAAGGGATTTATCTT+AGG	0.222166	8:+54931983	MsG0880045148.01.T01:CDS
AGAAGTGGATCAAAAGTTTC+AGG	0.259324	8:+54932153	MsG0880045148.01.T01:CDS
AGCAACTGCCAATGCTGATT+TGG	0.280391	8:-54932188	None:intergenic
AATTGCCTTTGTTACTTTCA+AGG	0.314569	8:+54928772	MsG0880045148.01.T01:CDS
TGGAAAATGGATGGATTATC+AGG	0.363124	8:+54932737	MsG0880045148.01.T01:three_prime_UTR
ACTGGTACTACCTGGGTAAC+TGG	0.368921	8:+54932265	MsG0880045148.01.T01:CDS
AATTTGCAGGAGTTTCAAAA+AGG	0.375052	8:-54932501	None:intergenic
AAGCATATCATTTCCAACTT+TGG	0.375062	8:-54932621	None:intergenic
AGAAACTGAATTTCCAAAGT+TGG	0.404196	8:+54932608	MsG0880045148.01.T01:three_prime_UTR
AAAAGTCGGTAATGTTTCCT+TGG	0.410188	8:+54928551	MsG0880045148.01.T01:CDS
AGTAAGGTTGCTAAGGCAGC+TGG	0.411693	8:+54932295	MsG0880045148.01.T01:CDS
AAAATGAAAAGAACTCCTTT+AGG	0.412585	8:-54928590	None:intergenic
TTGCGAAGGGATTTATCTTA+GGG	0.414214	8:+54931984	MsG0880045148.01.T01:CDS
CCGAGGCTGTTGAACTCAAC+TGG	0.416921	8:-54932048	None:intergenic
CTCACACTGAAACAATTTGC+AGG	0.422823	8:-54932514	None:intergenic
TTGAATATGTTGAAATGAAA+AGG	0.433319	8:+54928622	MsG0880045148.01.T01:CDS
TTCCAAGCCTACACCTGCTC+AGG	0.433849	8:+54932447	MsG0880045148.01.T01:CDS
CCAGTATGCGCAGGTCCTTT+CGG	0.448214	8:+54932387	MsG0880045148.01.T01:CDS
GTGGTGCCGACTCTGCTTTA+CGG	0.453078	8:+54931933	MsG0880045148.01.T01:CDS
AAAGTCGGTAATGTTTCCTT+GGG	0.468203	8:+54928552	MsG0880045148.01.T01:CDS
GCTAAGGCAGCTGGGGATGT+AGG	0.476446	8:+54932304	MsG0880045148.01.T01:CDS
CCGAAAGGACCTGCGCATAC+TGG	0.482179	8:-54932387	None:intergenic
CGATAGTAGTACTGCCGTCT+CGG	0.498280	8:-54928807	None:intergenic
ACCCTGAGCAGGTGTAGGCT+TGG	0.499327	8:-54932449	None:intergenic
GCCGCTGAGGGTAAAACTCC+TGG	0.513612	8:+54931911	MsG0880045148.01.T01:CDS
CAAAGGATCAAACCCTGAGC+AGG	0.517360	8:-54932460	None:intergenic
ACACAAGGGTGGAAAATGGA+TGG	0.525526	8:+54932728	MsG0880045148.01.T01:three_prime_UTR
ATATACTCACTGGTACTACC+TGG	0.533676	8:+54932257	MsG0880045148.01.T01:CDS
AGAAAAGACCAAATCAGCAT+TGG	0.537264	8:+54932180	MsG0880045148.01.T01:CDS
GAACAGAAAGTCAGTACCGC+AGG	0.537626	8:+54932211	MsG0880045148.01.T01:CDS
TTTACGGAAGGCAGAGGATG+TGG	0.541227	8:+54931949	MsG0880045148.01.T01:CDS
AGCATCTGAACAAGACCTAA+AGG	0.544372	8:+54928575	MsG0880045148.01.T01:CDS
GTAAGGTTGCTAAGGCAGCT+GGG	0.546936	8:+54932296	MsG0880045148.01.T01:CDS
GTCTTGTTCAGATGCTCCCA+AGG	0.552262	8:-54928568	None:intergenic
TCCAAGCCTACACCTGCTCA+GGG	0.564645	8:+54932448	MsG0880045148.01.T01:CDS
CCAGTTGAGTTCAACAGCCT+CGG	0.565333	8:+54932048	MsG0880045148.01.T01:CDS
AAAGCAGAGTCGGCACCACC+AGG	0.566278	8:-54931929	None:intergenic
AAATCCCTTCGCAAGCATGC+TGG	0.567408	8:-54931975	None:intergenic
CAGGTCAAAACTATAAAAGT+CGG	0.570204	8:+54928537	MsG0880045148.01.T01:CDS
GATGCTGTGAACAAAGCAAA+GGG	0.570764	8:+54932010	MsG0880045148.01.T01:CDS
GGTCACCAGCATGCTTGCGA+AGG	0.578828	8:+54931970	MsG0880045148.01.T01:CDS
GCAGAGATGGGTTCTTCACA+AGG	0.579774	8:+54928480	None:intergenic
GAGTGGTAGAGTTAAAGAAG+TGG	0.581041	8:+54932138	MsG0880045148.01.T01:CDS
CTCTGCTTTACGGAAGGCAG+AGG	0.586688	8:+54931943	MsG0880045148.01.T01:CDS
GGATCCACAAGGTGCCGAGA+CGG	0.588318	8:+54928793	MsG0880045148.01.T01:CDS
GTCACCAGCATGCTTGCGAA+GGG	0.591713	8:+54931971	MsG0880045148.01.T01:CDS
AGATGCTGTGAACAAAGCAA+AGG	0.591752	8:+54932009	MsG0880045148.01.T01:CDS
ACTGCCGTCTCGGCACCTTG+TGG	0.593659	8:-54928797	None:intergenic
TGCCGACTCTGCTTTACGGA+AGG	0.594224	8:+54931937	MsG0880045148.01.T01:CDS
AGTTGAAGACCAGTATGCGC+AGG	0.604810	8:+54932378	MsG0880045148.01.T01:CDS
TGCCTTCCGTAAAGCAGAGT+CGG	0.606291	8:-54931939	None:intergenic
TGGATCCTTGAAAGTAACAA+AGG	0.609650	8:-54928777	None:intergenic
ATGTACAAGAAGTAAATCAA+AGG	0.612134	8:-54932477	None:intergenic
CTTAGTGAGAAATTAACTGC+TGG	0.614592	8:+54932103	MsG0880045148.01.T01:CDS
ACTAAAAGCACCAGTTACCC+AGG	0.621013	8:-54932275	None:intergenic
TATACTCACTGGTACTACCT+GGG	0.629105	8:+54932258	MsG0880045148.01.T01:CDS
TAAGGTTGCTAAGGCAGCTG+GGG	0.631391	8:+54932297	MsG0880045148.01.T01:CDS
GTGCAGTTCACTTGTTGCTG+CGG	0.638692	8:-54932422	None:intergenic
CGAGACGGCAGTACTACTAT+CGG	0.643990	8:+54928808	MsG0880045148.01.T01:CDS
ATCAAACCCTGAGCAGGTGT+AGG	0.644936	8:-54932454	None:intergenic
GTTACTTTCAAGGATCCACA+AGG	0.648181	8:+54928782	MsG0880045148.01.T01:CDS
AACAGAAAGTCAGTACCGCA+GGG	0.666312	8:+54932212	MsG0880045148.01.T01:CDS
GCTGAGGGTAAAACTCCTGG+TGG	0.693763	8:+54931914	MsG0880045148.01.T01:CDS
AAAAGATGTAACTTTCGCCG+AGG	0.709369	8:-54932065	None:intergenic
GCTGGTGCATCAGTTGTGAG+TGG	0.711756	8:+54932121	MsG0880045148.01.T01:CDS
TTTCATTATAGCAGACCCTG+CGG	0.741855	8:-54932227	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATCAATTAGATTAATA+GGG	-	Chr8:54930107-54930126	None:intergenic	10.0%
!!	GTATAATAAAACTTAATAAT+GGG	-	Chr8:54928693-54928712	None:intergenic	10.0%
!!	TGTATAATAAAACTTAATAA+TGG	-	Chr8:54928694-54928713	None:intergenic	10.0%
!!!	AAAATTTTATTTTTACACAA+GGG	+	Chr8:54932714-54932733	MsG0880045148.01.T01:three_prime_UTR	10.0%
!!!	TTTTTTATATATATAATTGC+TGG	-	Chr8:54931506-54931525	None:intergenic	10.0%
!!	AGAATTGAGAATAATAAAAT+AGG	+	Chr8:54929943-54929962	MsG0880045148.01.T01:intron	15.0%
!!	AGATTTAATATAAATTGAAG+GGG	+	Chr8:54930973-54930992	MsG0880045148.01.T01:intron	15.0%
!!	AGTATAAAATTGTATGAAAA+TGG	-	Chr8:54930054-54930073	None:intergenic	15.0%
!!	ATAAAATTGATCCTTAAAAT+AGG	+	Chr8:54931316-54931335	MsG0880045148.01.T01:intron	15.0%
!!	CAGATTTAATATAAATTGAA+GGG	+	Chr8:54930972-54930991	MsG0880045148.01.T01:intron	15.0%
!!	GAACAAATAAAATAGTTTAT+CGG	-	Chr8:54930170-54930189	None:intergenic	15.0%
!!	GAATTGAGAATAATAAAATA+GGG	+	Chr8:54929944-54929963	MsG0880045148.01.T01:intron	15.0%
!!	GATATATCAATTAGATTAAT+AGG	-	Chr8:54930108-54930127	None:intergenic	15.0%
!!	TCAGATTTAATATAAATTGA+AGG	+	Chr8:54930971-54930990	MsG0880045148.01.T01:intron	15.0%
!!	TTAATAATACTAATCATTGA+AGG	+	Chr8:54931377-54931396	MsG0880045148.01.T01:intron	15.0%
!!!	AAATATTACACATTTGTTAT+AGG	-	Chr8:54931886-54931905	None:intergenic	15.0%
!!!	GAAAATTTTATTTTTACACA+AGG	+	Chr8:54932713-54932732	MsG0880045148.01.T01:three_prime_UTR	15.0%
!!!	TCTGATTTATCATTTTATTT+GGG	+	Chr8:54931162-54931181	MsG0880045148.01.T01:intron	15.0%
!!	AACAAGAATAAAAAAGGAAA+AGG	-	Chr8:54930414-54930433	None:intergenic	20.0%
!!	AATAAGTTTACCAACATAAT+AGG	-	Chr8:54931244-54931263	None:intergenic	20.0%
!!	ACAAGAATAAAAAAGGAAAA+GGG	-	Chr8:54930413-54930432	None:intergenic	20.0%
!!	AGAAAACTGATATTTATTTG+AGG	+	Chr8:54929071-54929090	MsG0880045148.01.T01:intron	20.0%
!!	ATATTATATGTCTGATTCAT+GGG	+	Chr8:54929767-54929786	MsG0880045148.01.T01:intron	20.0%
!!	CATGTTTATATGAAATGAAT+AGG	-	Chr8:54929465-54929484	None:intergenic	20.0%
!!	GTAATGAATATATGATTCTT+TGG	-	Chr8:54928936-54928955	None:intergenic	20.0%
!!	TATATTATATGTCTGATTCA+TGG	+	Chr8:54929766-54929785	MsG0880045148.01.T01:intron	20.0%
!!	TATTCAGTTATATATCCTAT+TGG	-	Chr8:54930213-54930232	None:intergenic	20.0%
!!	TTGAATATGTTGAAATGAAA+AGG	+	Chr8:54928622-54928641	MsG0880045148.01.T01:CDS	20.0%
!!!	ACAAATGTGTAATATTTTTC+AGG	+	Chr8:54931889-54931908	MsG0880045148.01.T01:intron	20.0%
!!!	ACTTAACTAAAAACTGATTT+TGG	+	Chr8:54930492-54930511	MsG0880045148.01.T01:intron	20.0%
!!!	AGTTTTTAGTTAAGTTTTTG+TGG	-	Chr8:54930487-54930506	None:intergenic	20.0%
!!!	ATTATCAAAGTTGATCTTTT+TGG	+	Chr8:54930826-54930845	MsG0880045148.01.T01:intron	20.0%
!!!	ATTTGATGCATGTATATTTT+TGG	+	Chr8:54931433-54931452	MsG0880045148.01.T01:intron	20.0%
!!!	GTCTGATTTATCATTTTATT+TGG	+	Chr8:54931161-54931180	MsG0880045148.01.T01:intron	20.0%
!!!	TAAATCTGAAATTGAATGAT+TGG	-	Chr8:54930960-54930979	None:intergenic	20.0%
!!!	TTATATCTAACACTATTTTC+TGG	+	Chr8:54930672-54930691	MsG0880045148.01.T01:intron	20.0%
!!!	TTATTTTTTAGCACAAAGTA+GGG	-	Chr8:54929854-54929873	None:intergenic	20.0%
!	AAAATCTCAGAATTTCAGAT+AGG	-	Chr8:54929619-54929638	None:intergenic	25.0%
!	AAAATGAAAAGAACTCCTTT+AGG	-	Chr8:54928593-54928612	None:intergenic	25.0%
!	AAATCTCAGAATTTCAGATA+GGG	-	Chr8:54929618-54929637	None:intergenic	25.0%
!	AAATTGAAAAGAATAGCCTT+TGG	+	Chr8:54931100-54931119	MsG0880045148.01.T01:intron	25.0%
!	AAGAAACAAGTCACTTAATT+TGG	-	Chr8:54931463-54931482	None:intergenic	25.0%
!	AAGTTATTAAGTGGTTGATT+GGG	-	Chr8:54930368-54930387	None:intergenic	25.0%
!	ACAAAAAACAAAAGAGAAAG+TGG	+	Chr8:54932327-54932346	MsG0880045148.01.T01:CDS	25.0%
!	ACTGTATTACTGTATATTCT+CGG	+	Chr8:54930235-54930254	MsG0880045148.01.T01:intron	25.0%
!	ATATTCATTACTGAGATTTC+AGG	+	Chr8:54928945-54928964	MsG0880045148.01.T01:intron	25.0%
!	ATCCAAAAACTTAAGCTATT+GGG	+	Chr8:54929725-54929744	MsG0880045148.01.T01:intron	25.0%
!	ATGTACAAGAAGTAAATCAA+AGG	-	Chr8:54932480-54932499	None:intergenic	25.0%
!	CAGTCTAGAGAAAAATATAA+GGG	+	Chr8:54932659-54932678	MsG0880045148.01.T01:three_prime_UTR	25.0%
!	GAAATGAATATCCTAGTTAA+TGG	-	Chr8:54931799-54931818	None:intergenic	25.0%
!	GAATCTATATCATAAGAAAG+AGG	-	Chr8:54929596-54929615	None:intergenic	25.0%
!	GATATTCATTTCATCCTATT+GGG	+	Chr8:54931807-54931826	MsG0880045148.01.T01:intron	25.0%
!	GTGGTTAACAAGAATAAAAA+AGG	-	Chr8:54930420-54930439	None:intergenic	25.0%
!	GTTGCTTAATCTTTATGTTA+TGG	+	Chr8:54930803-54930822	MsG0880045148.01.T01:intron	25.0%
!	GTTTAGTGTTATGAAAGAAA+GGG	+	Chr8:54929484-54929503	MsG0880045148.01.T01:intron	25.0%
!	TAGAGAAAAATATAAGGGAA+TGG	+	Chr8:54932664-54932683	MsG0880045148.01.T01:three_prime_UTR	25.0%
!	TATCCAAAAACTTAAGCTAT+TGG	+	Chr8:54929724-54929743	MsG0880045148.01.T01:intron	25.0%
!	TATTATATGTCTGATTCATG+GGG	+	Chr8:54929768-54929787	MsG0880045148.01.T01:intron	25.0%
!	TATTCATTACTGAGATTTCA+GGG	+	Chr8:54928946-54928965	MsG0880045148.01.T01:intron	25.0%
!	TCAGTCTAGAGAAAAATATA+AGG	+	Chr8:54932658-54932677	MsG0880045148.01.T01:three_prime_UTR	25.0%
!	TCCTTAAAATAGGATCAAAT+TGG	+	Chr8:54931326-54931345	MsG0880045148.01.T01:intron	25.0%
!	TGTTTAGTGTTATGAAAGAA+AGG	+	Chr8:54929483-54929502	MsG0880045148.01.T01:intron	25.0%
!	TTTAGTGTTATGAAAGAAAG+GGG	+	Chr8:54929485-54929504	MsG0880045148.01.T01:intron	25.0%
!	TTTGGATAAGTGGTATAATA+TGG	+	Chr8:54931677-54931696	MsG0880045148.01.T01:intron	25.0%
!!	ACCAATTTGATCCTATTTTA+AGG	-	Chr8:54931330-54931349	None:intergenic	25.0%
!!	ATATTTTTCTCTAGACTGAA+TGG	-	Chr8:54932657-54932676	None:intergenic	25.0%
!!	ATTTTATCTTAAAGCAGTGA+AGG	-	Chr8:54930901-54930920	None:intergenic	25.0%
!!	TACAATTTTATACTCCCTAA+AGG	+	Chr8:54930060-54930079	MsG0880045148.01.T01:intron	25.0%
!!	TATTTTTCTCTAGACTGAAT+GGG	-	Chr8:54932656-54932675	None:intergenic	25.0%
!!	TTATGTTGGTAAACTTATTC+TGG	+	Chr8:54931245-54931264	MsG0880045148.01.T01:intron	25.0%
!!	TTTTATCTTAAAGCAGTGAA+GGG	-	Chr8:54930900-54930919	None:intergenic	25.0%
!!!	ACAGTAGTTGTCTTATTTTT+GGG	+	Chr8:54931064-54931083	MsG0880045148.01.T01:intron	25.0%
!!!	ATTTTATTTTTACACAAGGG+TGG	+	Chr8:54932717-54932736	MsG0880045148.01.T01:three_prime_UTR	25.0%
!!!	ATTTTGATATCACTGCTAAA+TGG	+	Chr8:54931403-54931422	MsG0880045148.01.T01:intron	25.0%
!!!	CTATTTTCTGGCAATTTTAT+TGG	+	Chr8:54930684-54930703	MsG0880045148.01.T01:intron	25.0%
!!!	GGTTTCATAAGTCTTTTTTT+TGG	+	Chr8:54929138-54929157	MsG0880045148.01.T01:intron	25.0%
!!!	GTTACATGTATTTTATTTGC+AGG	+	Chr8:54928518-54928537	MsG0880045148.01.T01:CDS	25.0%
!!!	GTTATTTTTTAGCACAAAGT+AGG	-	Chr8:54929855-54929874	None:intergenic	25.0%
!!!	TGTTCTATTTTGATAGAAAC+TGG	+	Chr8:54930185-54930204	MsG0880045148.01.T01:intron	25.0%
!!!	TTGTGTTTTTATCTCTGATT+TGG	-	Chr8:54930271-54930290	None:intergenic	25.0%
	AAAAATAACGACATCGAACA+CGG	-	Chr8:54929304-54929323	None:intergenic	30.0%
	AAAAATCGCTATATACTCAC+TGG	+	Chr8:54932247-54932266	MsG0880045148.01.T01:CDS	30.0%
	AAATAAGACAACTACTGTCT+AGG	-	Chr8:54931062-54931081	None:intergenic	30.0%
	AAGCATATCATTTCCAACTT+TGG	-	Chr8:54932624-54932643	None:intergenic	30.0%
	AATTTGCAGGAGTTTCAAAA+AGG	-	Chr8:54932504-54932523	None:intergenic	30.0%
	ACAATCCAAGACAAATTGAT+TGG	-	Chr8:54928910-54928929	None:intergenic	30.0%
	ACCAACTAAGTATACCAAAT+AGG	-	Chr8:54930723-54930742	None:intergenic	30.0%
	AGAAACTGAATTTCCAAAGT+TGG	+	Chr8:54932608-54932627	MsG0880045148.01.T01:three_prime_UTR	30.0%
	AGATTGAACTCTAGATCAAA+TGG	+	Chr8:54929902-54929921	MsG0880045148.01.T01:intron	30.0%
	AGGGAGAATAATGATAGAAA+GGG	-	Chr8:54930088-54930107	None:intergenic	30.0%
	AGTTAATGGTATGTTTGGAA+TGG	-	Chr8:54931785-54931804	None:intergenic	30.0%
	AGTTTATCGGTATATCAGTT+TGG	-	Chr8:54930157-54930176	None:intergenic	30.0%
	ATCAAAGCCTCTATATTAAG+AGG	-	Chr8:54930589-54930608	None:intergenic	30.0%
	ATCCTAGTTAATGGTATGTT+TGG	-	Chr8:54931790-54931809	None:intergenic	30.0%
	ATCTTCTCCTCTTAATATAG+AGG	+	Chr8:54930579-54930598	MsG0880045148.01.T01:intron	30.0%
	ATGAACTCATTTCATCCTAT+TGG	+	Chr8:54931740-54931759	MsG0880045148.01.T01:intron	30.0%
	ATTAACTGATGATCAATGTC+AGG	-	Chr8:54928841-54928860	None:intergenic	30.0%
	ATTTGTCAGTAACATAACTC+TGG	+	Chr8:54931569-54931588	MsG0880045148.01.T01:intron	30.0%
	CAATCCAAGACAAATTGATT+GGG	-	Chr8:54928909-54928928	None:intergenic	30.0%
	CAGGTCAAAACTATAAAAGT+CGG	+	Chr8:54928537-54928556	MsG0880045148.01.T01:CDS	30.0%
	GAAGTTATTAAGTGGTTGAT+TGG	-	Chr8:54930369-54930388	None:intergenic	30.0%
	GGAGAATTCTTTCTTCTTTA+TGG	-	Chr8:54930020-54930039	None:intergenic	30.0%
	GGATATTCATTTCATCCTAT+TGG	+	Chr8:54931806-54931825	MsG0880045148.01.T01:intron	30.0%
	GGCAAAAATATCCTAGTTAA+TGG	-	Chr8:54931865-54931884	None:intergenic	30.0%
	GTTGTCTAGAAGTTATTAAG+TGG	-	Chr8:54930377-54930396	None:intergenic	30.0%
	TAGGGAGAATAATGATAGAA+AGG	-	Chr8:54930089-54930108	None:intergenic	30.0%
	TGAACTCATTTCATCCTATT+GGG	+	Chr8:54931741-54931760	MsG0880045148.01.T01:intron	30.0%
	TTAGACCTCTCATAAAAGAT+TGG	-	Chr8:54930558-54930577	None:intergenic	30.0%
	TTCCAAACATACCATTAACT+AGG	+	Chr8:54931785-54931804	MsG0880045148.01.T01:intron	30.0%
	TTGTTGACATCCTATTATGT+TGG	+	Chr8:54931231-54931250	MsG0880045148.01.T01:intron	30.0%
!	AAACTGATAATGCAGCATAA+GGG	+	Chr8:54929117-54929136	MsG0880045148.01.T01:intron	30.0%
!	AAGGAGTTCTTTTCATTTTC+TGG	+	Chr8:54928594-54928613	MsG0880045148.01.T01:CDS	30.0%
!	ACATCTTTTGACCAAAAACT+TGG	+	Chr8:54932079-54932098	MsG0880045148.01.T01:CDS	30.0%
!	ACTCTTATTTAGACCAATAG+AGG	+	Chr8:54929873-54929892	MsG0880045148.01.T01:intron	30.0%
!	AGTCAATAGCTTAAGCTTTT+GGG	-	Chr8:54930633-54930652	None:intergenic	30.0%
!	ATGTTTGGAATGGTGATATA+AGG	-	Chr8:54931775-54931794	None:intergenic	30.0%
!	CATCTTTTGACCAAAAACTT+GGG	+	Chr8:54932080-54932099	MsG0880045148.01.T01:CDS	30.0%
!	GACAGATTTTACTAGTTACT+TGG	+	Chr8:54932546-54932565	MsG0880045148.01.T01:three_prime_UTR	30.0%
!	GTGTTGACAAAATGAAACAT+AGG	-	Chr8:54929555-54929574	None:intergenic	30.0%
!	TATAGCCAATCTTTTATGAG+AGG	+	Chr8:54930550-54930569	MsG0880045148.01.T01:intron	30.0%
!	TCTCATAAAAGATTGGCTAT+AGG	-	Chr8:54930551-54930570	None:intergenic	30.0%
!	TTTCATTTTGTCAACACCAA+GGG	+	Chr8:54929558-54929577	MsG0880045148.01.T01:intron	30.0%
!!	AAACTGATTTTGGTCAGTAT+TGG	+	Chr8:54930502-54930521	MsG0880045148.01.T01:intron	30.0%
!!	AAGTTTTTCAACATATCAGG+GGG	+	Chr8:54931534-54931553	MsG0880045148.01.T01:intron	30.0%
!!	AATTGCCTTTGTTACTTTCA+AGG	+	Chr8:54928772-54928791	MsG0880045148.01.T01:CDS	30.0%
!!	ACCAAGTTTTTCAACATATC+AGG	+	Chr8:54931531-54931550	MsG0880045148.01.T01:intron	30.0%
!!	AGCTTAAGTTTTTGGATAGT+TGG	-	Chr8:54929722-54929741	None:intergenic	30.0%
!!	CAAGTTTTTCAACATATCAG+GGG	+	Chr8:54931533-54931552	MsG0880045148.01.T01:intron	30.0%
!!	CCAAGTTTTTCAACATATCA+GGG	+	Chr8:54931532-54931551	MsG0880045148.01.T01:intron	30.0%
!!	GTGACTTGTTTCTTTAATGT+AGG	+	Chr8:54931469-54931488	MsG0880045148.01.T01:intron	30.0%
!!	TACTGATGTTAATTTGTGCT+TGG	+	Chr8:54931024-54931043	MsG0880045148.01.T01:intron	30.0%
!!!	AGTTGACTTGTTTAGCTTTT+TGG	+	Chr8:54929019-54929038	MsG0880045148.01.T01:intron	30.0%
!!!	GACAGTAGTTGTCTTATTTT+TGG	+	Chr8:54931063-54931082	MsG0880045148.01.T01:intron	30.0%
	AAAAGTCGGTAATGTTTCCT+TGG	+	Chr8:54928551-54928570	MsG0880045148.01.T01:CDS	35.0%
	AAAATAACGACATCGAACAC+GGG	-	Chr8:54929303-54929322	None:intergenic	35.0%
	AAAGTCGGTAATGTTTCCTT+GGG	+	Chr8:54928552-54928571	MsG0880045148.01.T01:CDS	35.0%
	AAATAGGATCAAATTGGTCC+TGG	+	Chr8:54931332-54931351	MsG0880045148.01.T01:intron	35.0%
	AAGAAAGAATTCTCCACCAA+GGG	+	Chr8:54930025-54930044	MsG0880045148.01.T01:intron	35.0%
	AAGCTAAACAAGTCAACTCA+AGG	-	Chr8:54929017-54929036	None:intergenic	35.0%
	AATCCTTTGCTTGTTCAGTT+TGG	+	Chr8:54929375-54929394	MsG0880045148.01.T01:intron	35.0%
	ACTTATCACCAAAAAAGGCA+GGG	-	Chr8:54929179-54929198	None:intergenic	35.0%
	AGAAAAGACCAAATCAGCAT+TGG	+	Chr8:54932180-54932199	MsG0880045148.01.T01:CDS	35.0%
	AGAAGTGGATCAAAAGTTTC+AGG	+	Chr8:54932153-54932172	MsG0880045148.01.T01:CDS	35.0%
	ATGTCACTGATATCTATGAG+AGG	-	Chr8:54931288-54931307	None:intergenic	35.0%
	ATGTTTGTCAATTATGTGCG+TGG	-	Chr8:54930439-54930458	None:intergenic	35.0%
	CCCTGATATGTTGAAAAACT+TGG	-	Chr8:54931535-54931554	None:intergenic	35.0%
	CTTAGTGAGAAATTAACTGC+TGG	+	Chr8:54932103-54932122	MsG0880045148.01.T01:CDS	35.0%
	CTTTGTTGAGCGTAAATATC+AGG	+	Chr8:54929641-54929660	MsG0880045148.01.T01:intron	35.0%
	GCCTATTTGGTATACTTAGT+TGG	+	Chr8:54930719-54930738	MsG0880045148.01.T01:intron	35.0%
	GCTACTAGAACTAGAAGAAT+GGG	+	Chr8:54930929-54930948	MsG0880045148.01.T01:intron	35.0%
	GGAGAATAATGATAGAAAGG+GGG	-	Chr8:54930086-54930105	None:intergenic	35.0%
	GGGAGAATAATGATAGAAAG+GGG	-	Chr8:54930087-54930106	None:intergenic	35.0%
	GTATTACTGTATATTCTCGG+AGG	+	Chr8:54930238-54930257	MsG0880045148.01.T01:intron	35.0%
	GTTAATATGTGTATCACGCA+TGG	+	Chr8:54928855-54928874	MsG0880045148.01.T01:intron	35.0%
	TACTTATCACCAAAAAAGGC+AGG	-	Chr8:54929180-54929199	None:intergenic	35.0%
	TATTAAGTGGTTGATTGGGA+AGG	-	Chr8:54930364-54930383	None:intergenic	35.0%
	TCAATCTCTGTTTCCTCTAT+TGG	-	Chr8:54929889-54929908	None:intergenic	35.0%
	TGATAGAAACTGGTACCAAT+AGG	+	Chr8:54930195-54930214	MsG0880045148.01.T01:intron	35.0%
	TGCTACTAGAACTAGAAGAA+TGG	+	Chr8:54930928-54930947	MsG0880045148.01.T01:intron	35.0%
	TGGATCCTTGAAAGTAACAA+AGG	-	Chr8:54928780-54928799	None:intergenic	35.0%
	TGTATGAAAATGGAAACCCT+TGG	-	Chr8:54930044-54930063	None:intergenic	35.0%
	TTGAGCGTAAATATCAGGTT+TGG	+	Chr8:54929646-54929665	MsG0880045148.01.T01:intron	35.0%
	TTGCGAAGGGATTTATCTTA+GGG	+	Chr8:54931984-54932003	MsG0880045148.01.T01:CDS	35.0%
!	AGGGTACTTATCACCAAAAA+AGG	-	Chr8:54929184-54929203	None:intergenic	35.0%
!	CACCCAATAGCTTAAGTTTT+TGG	-	Chr8:54929730-54929749	None:intergenic	35.0%
!	CAGTCAATAGCTTAAGCTTT+TGG	-	Chr8:54930634-54930653	None:intergenic	35.0%
!	CATAAAAGATTGGCTATAGG+AGG	-	Chr8:54930548-54930567	None:intergenic	35.0%
!	GAAACTGATAATGCAGCATA+AGG	+	Chr8:54929116-54929135	MsG0880045148.01.T01:intron	35.0%
!	GTGTGTCATTATCCGAATAA+AGG	+	Chr8:54928986-54929005	MsG0880045148.01.T01:intron	35.0%
!	GTTATGAAAGAAAGGGGTTT+TGG	+	Chr8:54929491-54929510	MsG0880045148.01.T01:intron	35.0%
!	GTTTCATTTTGTCAACACCA+AGG	+	Chr8:54929557-54929576	MsG0880045148.01.T01:intron	35.0%
!	TATGTGTCAGCTACTTTCTT+AGG	+	Chr8:54929415-54929434	MsG0880045148.01.T01:intron	35.0%
!	TGCTTTTAGTAAGGTTGCTA+AGG	+	Chr8:54932288-54932307	MsG0880045148.01.T01:CDS	35.0%
!	TTTGGAATGGTGATATAAGG+TGG	-	Chr8:54931772-54931791	None:intergenic	35.0%
!	TTTTACACAAGGGTGGAAAA+TGG	+	Chr8:54932724-54932743	MsG0880045148.01.T01:three_prime_UTR	35.0%
!!	AATTTGTGCTTGGTTTGCAT+AGG	+	Chr8:54931034-54931053	MsG0880045148.01.T01:intron	35.0%
!!	TGGAAAATGGATGGATTATC+AGG	+	Chr8:54932737-54932756	MsG0880045148.01.T01:three_prime_UTR	35.0%
!!	TTTTTAGCACAAAGTAGGGT+GGG	-	Chr8:54929850-54929869	None:intergenic	35.0%
!!!	TTTTTTAGCACAAAGTAGGG+TGG	-	Chr8:54929851-54929870	None:intergenic	35.0%
	AAAAGATGTAACTTTCGCCG+AGG	-	Chr8:54932068-54932087	None:intergenic	40.0%
	ACACATATATGCAGCCTCTA+AGG	-	Chr8:54929402-54929421	None:intergenic	40.0%
	ACTGTATATTCTCGGAGGAT+AGG	+	Chr8:54930243-54930262	MsG0880045148.01.T01:intron	40.0%
	AGAATCAAGCTCTCGAAACT+TGG	-	Chr8:54930339-54930358	None:intergenic	40.0%
	AGATGCTGTGAACAAAGCAA+AGG	+	Chr8:54932009-54932028	MsG0880045148.01.T01:CDS	40.0%
	AGCACCCAATCAATTTGTCT+TGG	+	Chr8:54928902-54928921	MsG0880045148.01.T01:intron	40.0%
	AGCATCTGAACAAGACCTAA+AGG	+	Chr8:54928575-54928594	MsG0880045148.01.T01:CDS	40.0%
	AGGCAACATCATACTTCCAA+AGG	-	Chr8:54931119-54931138	None:intergenic	40.0%
	AGTACCCTCCATTTCTCAAA+AGG	+	Chr8:54929196-54929215	MsG0880045148.01.T01:intron	40.0%
	AGTTAATGGTGTGCTTGGAA+TGG	-	Chr8:54931851-54931870	None:intergenic	40.0%
	ATAAGGTGGAATAGCCCAAT+AGG	-	Chr8:54931758-54931777	None:intergenic	40.0%
	ATATACTCACTGGTACTACC+TGG	+	Chr8:54932257-54932276	MsG0880045148.01.T01:CDS	40.0%
	ATCCTAGTTAATGGTGTGCT+TGG	-	Chr8:54931856-54931875	None:intergenic	40.0%
	ATGAAAATGGAAACCCTTGG+TGG	-	Chr8:54930041-54930060	None:intergenic	40.0%
	ATTCATGTGATGTCTGATGC+TGG	+	Chr8:54930866-54930885	MsG0880045148.01.T01:intron	40.0%
	ATTGGGTGAAGACACACAAA+TGG	+	Chr8:54929742-54929761	MsG0880045148.01.T01:intron	40.0%
	CCATGTTCAGAGGAAAGATT+AGG	+	Chr8:54930744-54930763	MsG0880045148.01.T01:intron	40.0%
	CCTAATCTTTCCTCTGAACA+TGG	-	Chr8:54930747-54930766	None:intergenic	40.0%
	CCTCTAGTTAATGTGCTCAA+GGG	+	Chr8:54931650-54931669	MsG0880045148.01.T01:intron	40.0%
	CCTTGAGCACATTAACTAGA+GGG	-	Chr8:54931652-54931671	None:intergenic	40.0%
	CTCAAGGCAATGCCTTTATT+CGG	-	Chr8:54929001-54929020	None:intergenic	40.0%
	CTCACACTGAAACAATTTGC+AGG	-	Chr8:54932517-54932536	None:intergenic	40.0%
	CTTATCACCAAAAAAGGCAG+GGG	-	Chr8:54929178-54929197	None:intergenic	40.0%
	CTTGCGAAGGGATTTATCTT+AGG	+	Chr8:54931983-54932002	MsG0880045148.01.T01:CDS	40.0%
	CTTGGAATGGTGATATAAGG+TGG	-	Chr8:54931838-54931857	None:intergenic	40.0%
	GAAGAAAGAATTCTCCACCA+AGG	+	Chr8:54930024-54930043	MsG0880045148.01.T01:intron	40.0%
	GAGTGGTAGAGTTAAAGAAG+TGG	+	Chr8:54932138-54932157	MsG0880045148.01.T01:CDS	40.0%
	GATATGCATCTCTGCCTATT+TGG	+	Chr8:54930706-54930725	MsG0880045148.01.T01:intron	40.0%
	GATGCTGTGAACAAAGCAAA+GGG	+	Chr8:54932010-54932029	MsG0880045148.01.T01:CDS	40.0%
	GTGCTTGGAATGGTGATATA+AGG	-	Chr8:54931841-54931860	None:intergenic	40.0%
	TACAATGTGACAAGCTGAGA+TGG	-	Chr8:54929279-54929298	None:intergenic	40.0%
	TATACTCACTGGTACTACCT+GGG	+	Chr8:54932258-54932277	MsG0880045148.01.T01:CDS	40.0%
	TGCACAATACGAACTAACAG+TGG	-	Chr8:54929531-54929550	None:intergenic	40.0%
	TTCCAAGCACACCATTAACT+AGG	+	Chr8:54931851-54931870	MsG0880045148.01.T01:intron	40.0%
	TTGTTCAGTTTGGACCTTAG+AGG	+	Chr8:54929385-54929404	MsG0880045148.01.T01:intron	40.0%
	TTTCATTATAGCAGACCCTG+CGG	-	Chr8:54932230-54932249	None:intergenic	40.0%
!	AAGATTAGGACTGTGCTGTT+AGG	+	Chr8:54930758-54930777	MsG0880045148.01.T01:intron	40.0%
!	AATGTGCTCAAGGGTATGTT+TGG	+	Chr8:54931659-54931678	MsG0880045148.01.T01:intron	40.0%
!	AGCTACTTTCTTAGGCAGTT+AGG	+	Chr8:54929423-54929442	MsG0880045148.01.T01:intron	40.0%
!	ATTTTCACCCCTGCCTTTTT+TGG	+	Chr8:54929168-54929187	MsG0880045148.01.T01:intron	40.0%
!	CAAGGGTATGTTTGGATAAG+TGG	+	Chr8:54931667-54931686	MsG0880045148.01.T01:intron	40.0%
!	GGTAACTGGTGCTTTTAGTA+AGG	+	Chr8:54932279-54932298	MsG0880045148.01.T01:CDS	40.0%
!	GTTACTTTCAAGGATCCACA+AGG	+	Chr8:54928782-54928801	MsG0880045148.01.T01:CDS	40.0%
!	TCTCACTAAGCCCAAGTTTT+TGG	-	Chr8:54932093-54932112	None:intergenic	40.0%
!	TTAGTTGGTTCCATGTTCAG+AGG	+	Chr8:54930734-54930753	MsG0880045148.01.T01:intron	40.0%
!!	AAACGCCTTTTGAGAAATGG+AGG	-	Chr8:54929204-54929223	None:intergenic	40.0%
!!	AACGCCTTTTGAGAAATGGA+GGG	-	Chr8:54929203-54929222	None:intergenic	40.0%
!!	GAGAAACGCCTTTTGAGAAA+TGG	-	Chr8:54929207-54929226	None:intergenic	40.0%
!!	TTAACTAGAGGGTGTTTACG+AGG	-	Chr8:54931641-54931660	None:intergenic	40.0%
!!!	AATATTTTTCAGGCCGCTGA+GGG	+	Chr8:54931899-54931918	MsG0880045148.01.T01:intron	40.0%
!!!	TAATATTTTTCAGGCCGCTG+AGG	+	Chr8:54931898-54931917	MsG0880045148.01.T01:intron	40.0%
	AACAGAAAGTCAGTACCGCA+GGG	+	Chr8:54932212-54932231	MsG0880045148.01.T01:CDS	45.0%
	ACACAAGGGTGGAAAATGGA+TGG	+	Chr8:54932728-54932747	MsG0880045148.01.T01:three_prime_UTR	45.0%
	AGGATCAAATTGGTCCTGGA+AGG	+	Chr8:54931336-54931355	MsG0880045148.01.T01:intron	45.0%
	ATGATAGAAAGGGGGCCTTT+AGG	-	Chr8:54930078-54930097	None:intergenic	45.0%
	CCCTCTAGTTAATGTGCTCA+AGG	+	Chr8:54931649-54931668	MsG0880045148.01.T01:intron	45.0%
	CCCTTGAGCACATTAACTAG+AGG	-	Chr8:54931653-54931672	None:intergenic	45.0%
	GAGGTTCACAATGAAACCCT+TGG	-	Chr8:54929577-54929596	None:intergenic	45.0%
	GCAGGTGGAAGTTTGTAATC+TGG	-	Chr8:54931597-54931616	None:intergenic	45.0%
	GGCTAGATAACGCAGCATAA+GGG	+	Chr8:54929040-54929059	MsG0880045148.01.T01:intron	45.0%
	GGGATAGCTAATTCACGACA+TGG	-	Chr8:54930613-54930632	None:intergenic	45.0%
	GGTCCAAACTGAACAAGCAA+AGG	-	Chr8:54929381-54929400	None:intergenic	45.0%
	TCAAGCTCTCGAAACTTGGT+AGG	-	Chr8:54930335-54930354	None:intergenic	45.0%
	TCAGACACATCCCAAAGTAG+AGG	-	Chr8:54931147-54931166	None:intergenic	45.0%
	TGAGAGGTTGCATACATCGT+TGG	-	Chr8:54931272-54931291	None:intergenic	45.0%
	TGATAGAAAGGGGGCCTTTA+GGG	-	Chr8:54930077-54930096	None:intergenic	45.0%
	TGCCTAAGTGCCTCTACTTT+GGG	+	Chr8:54931134-54931153	MsG0880045148.01.T01:intron	45.0%
	TGGCTAGATAACGCAGCATA+AGG	+	Chr8:54929039-54929058	MsG0880045148.01.T01:intron	45.0%
	TGTCTACACTTCCAGACCAA+GGG	-	Chr8:54929818-54929837	None:intergenic	45.0%
	TTGCCTAAGTGCCTCTACTT+TGG	+	Chr8:54931133-54931152	MsG0880045148.01.T01:intron	45.0%
	TTGTCTACACTTCCAGACCA+AGG	-	Chr8:54929819-54929838	None:intergenic	45.0%
!	AGCAACTGCCAATGCTGATT+TGG	-	Chr8:54932191-54932210	None:intergenic	45.0%
!	CACAGAAAGTGCAGTAGACA+CGG	+	Chr8:54930307-54930326	MsG0880045148.01.T01:intron	45.0%
!	TGGAAGTGTAGACAATGCAC+AGG	+	Chr8:54929824-54929843	MsG0880045148.01.T01:intron	45.0%
!!	ACTAAAAGCACCAGTTACCC+AGG	-	Chr8:54932278-54932297	None:intergenic	45.0%
!!	ATCCCAAAGTAGAGGCACTT+AGG	-	Chr8:54931139-54931158	None:intergenic	45.0%
!!	TTGGTCAGTATTGGCCTTCT+CGG	+	Chr8:54930511-54930530	MsG0880045148.01.T01:intron	45.0%
	AAATCCCTTCGCAAGCATGC+TGG	-	Chr8:54931978-54931997	None:intergenic	50.0%
	ACAGATGATGCCAAAGCAGC+AGG	-	Chr8:54931615-54931634	None:intergenic	50.0%
	ACCAAGGGCTCTTACGTTAG+TGG	-	Chr8:54929803-54929822	None:intergenic	50.0%
	ACTGGTACTACCTGGGTAAC+TGG	+	Chr8:54932265-54932284	MsG0880045148.01.T01:CDS	50.0%
	AGTAAGGTTGCTAAGGCAGC+TGG	+	Chr8:54932295-54932314	MsG0880045148.01.T01:CDS	50.0%
	AGTTGAAGACCAGTATGCGC+AGG	+	Chr8:54932378-54932397	MsG0880045148.01.T01:CDS	50.0%
	ATCAAACCCTGAGCAGGTGT+AGG	-	Chr8:54932457-54932476	None:intergenic	50.0%
	CAAAGGATCAAACCCTGAGC+AGG	-	Chr8:54932463-54932482	None:intergenic	50.0%
	CCAAGGGCTCTTACGTTAGT+GGG	-	Chr8:54929802-54929821	None:intergenic	50.0%
	CCAGTTGAGTTCAACAGCCT+CGG	+	Chr8:54932048-54932067	MsG0880045148.01.T01:CDS	50.0%
	CGAGACGGCAGTACTACTAT+CGG	+	Chr8:54928808-54928827	MsG0880045148.01.T01:CDS	50.0%
	CGATAGTAGTACTGCCGTCT+CGG	-	Chr8:54928810-54928829	None:intergenic	50.0%
	CGTGTCTACTGCACTTTCTG+TGG	-	Chr8:54930309-54930328	None:intergenic	50.0%
	GAACAGAAAGTCAGTACCGC+AGG	+	Chr8:54932211-54932230	MsG0880045148.01.T01:CDS	50.0%
	GGCTATAGGAGGAGAGACAA+AGG	-	Chr8:54930537-54930556	None:intergenic	50.0%
	GTAAGGTTGCTAAGGCAGCT+GGG	+	Chr8:54932296-54932315	MsG0880045148.01.T01:CDS	50.0%
	TAACGTAAGAGCCCTTGGTC+TGG	+	Chr8:54929804-54929823	MsG0880045148.01.T01:intron	50.0%
	TAAGGTTGCTAAGGCAGCTG+GGG	+	Chr8:54932297-54932316	MsG0880045148.01.T01:CDS	50.0%
	TGCCTTCCGTAAAGCAGAGT+CGG	-	Chr8:54931942-54931961	None:intergenic	50.0%
	TGGTCCTGGAAGGATGAAAC+TGG	+	Chr8:54931346-54931365	MsG0880045148.01.T01:intron	50.0%
	TTTACGGAAGGCAGAGGATG+TGG	+	Chr8:54931949-54931968	MsG0880045148.01.T01:CDS	50.0%
!	ACCAGGAGTTTTACCCTCAG+CGG	-	Chr8:54931915-54931934	None:intergenic	50.0%
!	CAAACTTCCACCTGCTGCTT+TGG	+	Chr8:54931602-54931621	MsG0880045148.01.T01:intron	50.0%
!	GCTCTTACGTTAGTGGGCAA+GGG	-	Chr8:54929796-54929815	None:intergenic	50.0%
!	GTCTTGTTCAGATGCTCCCA+AGG	-	Chr8:54928571-54928590	None:intergenic	50.0%
!	GTGCAGTTCACTTGTTGCTG+CGG	-	Chr8:54932425-54932444	None:intergenic	50.0%
!!	ACTTGTTGCTGCGGCTTTTG+GGG	-	Chr8:54932416-54932435	None:intergenic	50.0%
!!	CACTTGTTGCTGCGGCTTTT+GGG	-	Chr8:54932417-54932436	None:intergenic	50.0%
!!	TCACTTGTTGCTGCGGCTTT+TGG	-	Chr8:54932418-54932437	None:intergenic	50.0%
	AGGAGAGACAAAGGCCGAGA+AGG	-	Chr8:54930528-54930547	None:intergenic	55.0%
	CCAGTATGCGCAGGTCCTTT+CGG	+	Chr8:54932387-54932406	MsG0880045148.01.T01:CDS	55.0%
	CCCACTAACGTAAGAGCCCT+TGG	+	Chr8:54929799-54929818	MsG0880045148.01.T01:intron	55.0%
	CCGAGGCTGTTGAACTCAAC+TGG	-	Chr8:54932051-54932070	None:intergenic	55.0%
	CTCTGCTTTACGGAAGGCAG+AGG	+	Chr8:54931943-54931962	MsG0880045148.01.T01:CDS	55.0%
	GATGATGCCAAAGCAGCAGG+TGG	-	Chr8:54931612-54931631	None:intergenic	55.0%
	GCTGAGGGTAAAACTCCTGG+TGG	+	Chr8:54931914-54931933	MsG0880045148.01.T01:CDS	55.0%
	GCTGCCAGTTTCATCCTTCC+AGG	-	Chr8:54931353-54931372	None:intergenic	55.0%
	GCTGGTGCATCAGTTGTGAG+TGG	+	Chr8:54932121-54932140	MsG0880045148.01.T01:CDS	55.0%
	GGTCAGTGAGTTGCTGTGCT+TGG	+	Chr8:54928643-54928662	MsG0880045148.01.T01:intron	55.0%
	GTCACCAGCATGCTTGCGAA+GGG	+	Chr8:54931971-54931990	MsG0880045148.01.T01:CDS	55.0%
	TCCAAGCCTACACCTGCTCA+GGG	+	Chr8:54932448-54932467	MsG0880045148.01.T01:CDS	55.0%
	TTCCAAGCCTACACCTGCTC+AGG	+	Chr8:54932447-54932466	MsG0880045148.01.T01:CDS	55.0%
!	GGCTCTTACGTTAGTGGGCA+AGG	-	Chr8:54929797-54929816	None:intergenic	55.0%
!!	GTGGTGCCGACTCTGCTTTA+CGG	+	Chr8:54931933-54931952	MsG0880045148.01.T01:CDS	55.0%
!!	TGCCGACTCTGCTTTACGGA+AGG	+	Chr8:54931937-54931956	MsG0880045148.01.T01:CDS	55.0%
	AAAGCAGAGTCGGCACCACC+AGG	-	Chr8:54931932-54931951	None:intergenic	60.0%
	ACCCTGAGCAGGTGTAGGCT+TGG	-	Chr8:54932452-54932471	None:intergenic	60.0%
	CCGAAAGGACCTGCGCATAC+TGG	-	Chr8:54932390-54932409	None:intergenic	60.0%
	GCCGCTGAGGGTAAAACTCC+TGG	+	Chr8:54931911-54931930	MsG0880045148.01.T01:CDS	60.0%
	GCTAAGGCAGCTGGGGATGT+AGG	+	Chr8:54932304-54932323	MsG0880045148.01.T01:CDS	60.0%
	GGTCACCAGCATGCTTGCGA+AGG	+	Chr8:54931970-54931989	MsG0880045148.01.T01:CDS	60.0%
!!	CGGCTTTTGGGGACTCCGAA+AGG	-	Chr8:54932405-54932424	None:intergenic	60.0%
!!	GGATCCACAAGGTGCCGAGA+CGG	+	Chr8:54928793-54928812	MsG0880045148.01.T01:CDS	60.0%
	ACTGCCGTCTCGGCACCTTG+TGG	-	Chr8:54928800-54928819	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	54928486	54932772	54928486	ID=MsG0880045148.01;Name=MsG0880045148.01
Chr8	mRNA	54928486	54932772	54928486	ID=MsG0880045148.01.T01;Parent=MsG0880045148.01;Name=MsG0880045148.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|0.5\|0.66\|1\|1\|1\|3\|289\|268
Chr8	exon	54928486	54928643	54928486	ID=MsG0880045148.01.T01:exon:23684;Parent=MsG0880045148.01.T01
Chr8	exon	54928754	54928829	54928754	ID=MsG0880045148.01.T01:exon:23685;Parent=MsG0880045148.01.T01
Chr8	exon	54931911	54932772	54931911	ID=MsG0880045148.01.T01:exon:23686;Parent=MsG0880045148.01.T01
Chr8	CDS	54928486	54928643	54928486	ID=MsG0880045148.01.T01:cds;Parent=MsG0880045148.01.T01
Chr8	CDS	54928754	54928829	54928754	ID=MsG0880045148.01.T01:cds;Parent=MsG0880045148.01.T01
Chr8	CDS	54931911	54932483	54931911	ID=MsG0880045148.01.T01:cds;Parent=MsG0880045148.01.T01
Chr8	three_prime_UTR	54932484	54932772	54932484	ID=MsG0880045148.01.T01:three_prime_utr;Parent=MsG0880045148.01.T01

Gene Sequence

>MsG0880045148.01.T01

ATGGGTTCTTCACAAGGTTCTAAATGTTCAGTGTTACATGTATTTTATTTGCAGGTCAAAACTATAAAAGTCGGTAATGTTTCCTTGGGAGCATCTGAACAAGACCTAAAGGAGTTCTTTTCATTTTCTGGTGACATTGAATATGTTGAAATGAAAAGCTACGATGAACGATCTCAAATTGCCTTTGTTACTTTCAAGGATCCACAAGGTGCCGAGACGGCAGTACTACTATCGGCCGCTGAGGGTAAAACTCCTGGTGGTGCCGACTCTGCTTTACGGAAGGCAGAGGATGTGGTCACCAGCATGCTTGCGAAGGGATTTATCTTAGGGAAAGATGCTGTGAACAAAGCAAAGGGTTTTGATGAGAAGCACCAGTTGAGTTCAACAGCCTCGGCGAAAGTTACATCTTTTGACCAAAAACTTGGGCTTAGTGAGAAATTAACTGCTGGTGCATCAGTTGTGAGTGGTAGAGTTAAAGAAGTGGATCAAAAGTTTCAGGTTTCAGAAAAGACCAAATCAGCATTGGCAGTTGCTGAACAGAAAGTCAGTACCGCAGGGTCTGCTATAATGAAAAATCGCTATATACTCACTGGTACTACCTGGGTAACTGGTGCTTTTAGTAAGGTTGCTAAGGCAGCTGGGGATGTAGGACAAAAAACAAAAGAGAAAGTGGAAAATGCAGAAGTAGAACAGAATCGTAAAGTTGAAGACCAGTATGCGCAGGTCCTTTCGGAGTCCCCAAAAGCCGCAGCAACAAGTGAACTGCACTCTTCCAAGCCTACACCTGCTCAGGGTTTGATCCTTTGA

Protein sequence

>MsG0880045148.01.T01

MGSSQGSKCSVLHVFYLQVKTIKVGNVSLGASEQDLKEFFSFSGDIEYVEMKSYDERSQIAFVTFKDPQGAETAVLLSAAEGKTPGGADSALRKAEDVVTSMLAKGFILGKDAVNKAKGFDEKHQLSSTASAKVTSFDQKLGLSEKLTAGASVVSGRVKEVDQKFQVSEKTKSALAVAEQKVSTAGSAIMKNRYILTGTTWVTGAFSKVAKAAGDVGQKTKEKVENAEVEQNRKVEDQYAQVLSESPKAAATSELHSSKPTPAQGLIL*