AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005474.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005474.01.T01 MTR_1g100753 96.667 120 4 0 1 120 87 206 9.40e-82 238
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005474.01.T01 AT5G52190 75.610 123 27 1 1 120 86 208 3.32e-63 191

Find 42 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATGGTTGTGTATAAGTT+AGG 0.266389 1:+92237646 MsG0180005474.01.T01:CDS
TGACGAGATCGAACGGCTTC+AGG 0.341605 1:-92237688 None:intergenic
CTGACTGCTCAACCAGAGAT+AGG 0.342066 1:+92237484 MsG0180005474.01.T01:CDS
AGATGGTTGTGTATAAGTTA+GGG 0.370004 1:+92237647 MsG0180005474.01.T01:CDS
ATCAAGACCATTGCTTCCTA+TGG 0.384419 1:+92237588 MsG0180005474.01.T01:CDS
TCGATCTCGTCATACTAATC+TGG 0.390912 1:+92237699 MsG0180005474.01.T01:CDS
TTACGTGCCTGCAAAGACTA+TGG 0.402934 1:+92237537 MsG0180005474.01.T01:CDS
AGGAGATCATTGGTGGTGAT+TGG 0.410627 1:-92237376 None:intergenic
TCATTCGCCATAGTCTTTGC+AGG 0.428098 1:-92237544 None:intergenic
CTCATCGCCTCAGCCGGTCC+AGG 0.437578 1:+92237397 MsG0180005474.01.T01:CDS
TCGACAGTGGAGAAGCCGCC+TGG 0.438096 1:-92237415 None:intergenic
TTCACACAGGACCCTATCTC+TGG 0.470565 1:-92237496 None:intergenic
ACTGTTTGTGTTGTTTGAGA+TGG 0.478342 1:+92237630 MsG0180005474.01.T01:CDS
AGTGGAGAAGCCGCCTGGAC+CGG 0.488941 1:-92237410 None:intergenic
AAAGACTATGGCGAATGATA+AGG 0.495854 1:+92237549 MsG0180005474.01.T01:CDS
GATCTCCTCATCGCCTCAGC+CGG 0.497810 1:+92237391 MsG0180005474.01.T01:CDS
AGATCATTGGTGGTGATTGG+AGG 0.498257 1:-92237373 None:intergenic
ATGGCGAATGATAAGGATGA+AGG 0.506270 1:+92237556 MsG0180005474.01.T01:CDS
GAAGCCGCCTGGACCGGCTG+AGG 0.507477 1:-92237404 None:intergenic
TCAAGACCATTGCTTCCTAT+GGG 0.513407 1:+92237589 MsG0180005474.01.T01:CDS
CACTGTCGATGCTATATGCT+CGG 0.515036 1:+92237429 MsG0180005474.01.T01:CDS
AAGAAGCACTCTTCCACCGT+TGG 0.538774 1:-92237461 None:intergenic
TGAGGCGATGAGGAGATCAT+TGG 0.554801 1:-92237386 None:intergenic
TGACTGCTCAACCAGAGATA+GGG 0.560185 1:+92237485 MsG0180005474.01.T01:CDS
TGGCGAATGATAAGGATGAA+GGG 0.568337 1:+92237557 MsG0180005474.01.T01:CDS
CTGTGTGAAGCATGCTAGTG+TGG 0.570857 1:+92237510 MsG0180005474.01.T01:CDS
GGCGAATGATAAGGATGAAG+GGG 0.593461 1:+92237558 MsG0180005474.01.T01:CDS
CCTATGGGGAGTGTGTATGA+AGG 0.597763 1:+92237604 MsG0180005474.01.T01:CDS
GATGGTTGTGTATAAGTTAG+GGG 0.603756 1:+92237648 MsG0180005474.01.T01:CDS
TGCTCGGTAGCACGATCCAA+CGG 0.606651 1:+92237445 MsG0180005474.01.T01:CDS
CCTTCATACACACTCCCCAT+AGG 0.612186 1:-92237604 None:intergenic
ATTAGTATGACGAGATCGAA+CGG 0.612587 1:-92237695 None:intergenic
CTGGACCGGCTGAGGCGATG+AGG 0.614008 1:-92237396 None:intergenic
ACACTCCCCATAGGAAGCAA+TGG 0.614848 1:-92237595 None:intergenic
GGCGATGAGGAGATCATTGG+TGG 0.627157 1:-92237383 None:intergenic
ATTGGTGGTGATTGGAGGAG+TGG 0.628718 1:-92237368 None:intergenic
TGTGAAGCATGCTAGTGTGG+TGG 0.631703 1:+92237513 MsG0180005474.01.T01:CDS
CACACTAGCATGCTTCACAC+AGG 0.655929 1:-92237509 None:intergenic
CGAGCATATAGCATCGACAG+TGG 0.676439 1:-92237428 None:intergenic
CAAGACCATTGCTTCCTATG+GGG 0.677128 1:+92237590 MsG0180005474.01.T01:CDS
ATCGCCTCAGCCGGTCCAGG+CGG 0.693754 1:+92237400 MsG0180005474.01.T01:CDS
TCGGTAGCACGATCCAACGG+TGG 0.767760 1:+92237448 MsG0180005474.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGATGGTTGTGTATAAGTTA+GGG + Chr1:92237647-92237666 MsG0180005474.01.T01:CDS 30.0%
! TATAAGTTAGGGGAGATTTT+AGG + Chr1:92237658-92237677 MsG0180005474.01.T01:CDS 30.0%
AAAGACTATGGCGAATGATA+AGG + Chr1:92237549-92237568 MsG0180005474.01.T01:CDS 35.0%
ATTAGTATGACGAGATCGAA+CGG - Chr1:92237698-92237717 None:intergenic 35.0%
GAGATGGTTGTGTATAAGTT+AGG + Chr1:92237646-92237665 MsG0180005474.01.T01:CDS 35.0%
GATGGTTGTGTATAAGTTAG+GGG + Chr1:92237648-92237667 MsG0180005474.01.T01:CDS 35.0%
!! ACTGTTTGTGTTGTTTGAGA+TGG + Chr1:92237630-92237649 MsG0180005474.01.T01:CDS 35.0%
ATCAAGACCATTGCTTCCTA+TGG + Chr1:92237588-92237607 MsG0180005474.01.T01:CDS 40.0%
ATGGCGAATGATAAGGATGA+AGG + Chr1:92237556-92237575 MsG0180005474.01.T01:CDS 40.0%
TCAAGACCATTGCTTCCTAT+GGG + Chr1:92237589-92237608 MsG0180005474.01.T01:CDS 40.0%
TCGATCTCGTCATACTAATC+TGG + Chr1:92237699-92237718 MsG0180005474.01.T01:CDS 40.0%
TGGCGAATGATAAGGATGAA+GGG + Chr1:92237557-92237576 MsG0180005474.01.T01:CDS 40.0%
CAAGACCATTGCTTCCTATG+GGG + Chr1:92237590-92237609 MsG0180005474.01.T01:CDS 45.0%
CACTGTCGATGCTATATGCT+CGG + Chr1:92237429-92237448 MsG0180005474.01.T01:CDS 45.0%
GGCGAATGATAAGGATGAAG+GGG + Chr1:92237558-92237577 MsG0180005474.01.T01:CDS 45.0%
TCATTCGCCATAGTCTTTGC+AGG - Chr1:92237547-92237566 None:intergenic 45.0%
TGACTGCTCAACCAGAGATA+GGG + Chr1:92237485-92237504 MsG0180005474.01.T01:CDS 45.0%
TTACGTGCCTGCAAAGACTA+TGG + Chr1:92237537-92237556 MsG0180005474.01.T01:CDS 45.0%
!! AGATCATTGGTGGTGATTGG+AGG - Chr1:92237376-92237395 None:intergenic 45.0%
!! AGGAGATCATTGGTGGTGAT+TGG - Chr1:92237379-92237398 None:intergenic 45.0%
ACACTCCCCATAGGAAGCAA+TGG - Chr1:92237598-92237617 None:intergenic 50.0%
CACACTAGCATGCTTCACAC+AGG - Chr1:92237512-92237531 None:intergenic 50.0%
CCTTCATACACACTCCCCAT+AGG - Chr1:92237607-92237626 None:intergenic 50.0%
CTGACTGCTCAACCAGAGAT+AGG + Chr1:92237484-92237503 MsG0180005474.01.T01:CDS 50.0%
CTGTGTGAAGCATGCTAGTG+TGG + Chr1:92237510-92237529 MsG0180005474.01.T01:CDS 50.0%
TGAGGCGATGAGGAGATCAT+TGG - Chr1:92237389-92237408 None:intergenic 50.0%
TGTGAAGCATGCTAGTGTGG+TGG + Chr1:92237513-92237532 MsG0180005474.01.T01:CDS 50.0%
TTCACACAGGACCCTATCTC+TGG - Chr1:92237499-92237518 None:intergenic 50.0%
! CCTATGGGGAGTGTGTATGA+AGG + Chr1:92237604-92237623 MsG0180005474.01.T01:CDS 50.0%
!! AAGAAGCACTCTTCCACCGT+TGG - Chr1:92237464-92237483 None:intergenic 50.0%
!! ATTGGTGGTGATTGGAGGAG+TGG - Chr1:92237371-92237390 None:intergenic 50.0%
!! CGAGCATATAGCATCGACAG+TGG - Chr1:92237431-92237450 None:intergenic 50.0%
GGCGATGAGGAGATCATTGG+TGG - Chr1:92237386-92237405 None:intergenic 55.0%
TGACGAGATCGAACGGCTTC+AGG - Chr1:92237691-92237710 None:intergenic 55.0%
TGCTCGGTAGCACGATCCAA+CGG + Chr1:92237445-92237464 MsG0180005474.01.T01:CDS 55.0%
GATCTCCTCATCGCCTCAGC+CGG + Chr1:92237391-92237410 MsG0180005474.01.T01:CDS 60.0%
TCGGTAGCACGATCCAACGG+TGG + Chr1:92237448-92237467 MsG0180005474.01.T01:CDS 60.0%
AGTGGAGAAGCCGCCTGGAC+CGG - Chr1:92237413-92237432 None:intergenic 65.0%
TCGACAGTGGAGAAGCCGCC+TGG - Chr1:92237418-92237437 None:intergenic 65.0%
ATCGCCTCAGCCGGTCCAGG+CGG + Chr1:92237400-92237419 MsG0180005474.01.T01:CDS 70.0%
CTCATCGCCTCAGCCGGTCC+AGG + Chr1:92237397-92237416 MsG0180005474.01.T01:CDS 70.0%
CTGGACCGGCTGAGGCGATG+AGG - Chr1:92237399-92237418 None:intergenic 70.0%
GAAGCCGCCTGGACCGGCTG+AGG - Chr1:92237407-92237426 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 92237364 92237726 92237364 ID=MsG0180005474.01;Name=MsG0180005474.01
Chr1 mRNA 92237364 92237726 92237364 ID=MsG0180005474.01.T01;Parent=MsG0180005474.01;Name=MsG0180005474.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|120
Chr1 exon 92237364 92237726 92237364 ID=MsG0180005474.01.T01:exon:9852;Parent=MsG0180005474.01.T01
Chr1 CDS 92237364 92237726 92237364 ID=MsG0180005474.01.T01:cds;Parent=MsG0180005474.01.T01
Gene Sequence

>MsG0180005474.01.T01

ATGACCACTCCTCCAATCACCACCAATGATCTCCTCATCGCCTCAGCCGGTCCAGGCGGCTTCTCCACTGTCGATGCTATATGCTCGGTAGCACGATCCAACGGTGGAAGAGTGCTTCTTCTGACTGCTCAACCAGAGATAGGGTCCTGTGTGAAGCATGCTAGTGTGGTGGCTTACGTGCCTGCAAAGACTATGGCGAATGATAAGGATGAAGGGGATGTGAAATCAAGACCATTGCTTCCTATGGGGAGTGTGTATGAAGGTGCACTGTTTGTGTTGTTTGAGATGGTTGTGTATAAGTTAGGGGAGATTTTAGGTGAGAGTCCTGAAGCCGTTCGATCTCGTCATACTAATCTGGAATGA

Protein sequence

>MsG0180005474.01.T01

MTTPPITTNDLLIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPEIGSCVKHASVVAYVPAKTMANDKDEGDVKSRPLLPMGSVYEGALFVLFEMVVYKLGEILGESPEAVRSRHTNLE*