AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225000 MtrunA17_Chr2g0331241 81.633 98 10 1 2 91 409 506 1.74e-50 165
Msa0225000 MtrunA17_Chr8g0350901 94.118 51 3 0 41 91 11 61 7.06e-32 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225000 AT3G16230.13 59.783 92 28 1 3 85 229 320 5.62e-31 111
Msa0225000 AT3G16230.3 59.783 92 28 1 3 85 313 404 1.01e-30 112
Msa0225000 AT3G16230.12 59.783 92 28 1 3 85 313 404 1.01e-30 112
Msa0225000 AT3G16230.10 59.783 92 28 1 3 85 353 444 2.06e-30 111
Msa0225000 AT3G16230.2 59.783 92 28 1 3 85 353 444 2.06e-30 111
Msa0225000 AT3G16230.1 59.783 92 28 1 3 85 353 444 2.06e-30 111
Msa0225000 AT3G16220.1 46.591 88 40 2 4 85 169 255 7.09e-21 83.6

Find 14 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATAAGCCGCTGGTGTTC+AGG 0.332174 2_1:+75050803 Msa0225000:intron
CGAAACATATTCAAGCAGTA+TGG 0.390783 2_1:+75050862 Msa0225000:CDS
CGCTGGTGTTCAGGACAAAT+CGG 0.437383 2_1:+75050812 Msa0225000:CDS
TGTTAAAGCTGGACTTGTCT+TGG 0.438646 2_1:+75050114 Msa0225000:CDS
GCAGTATGGATCAGAGGACT+GGG 0.480693 2_1:+75050876 Msa0225000:CDS
ATTGATGCATATGTTAAAGC+TGG 0.482111 2_1:+75050103 Msa0225000:CDS
AGCAGTATGGATCAGAGGAC+TGG 0.508829 2_1:+75050875 Msa0225000:CDS
TGATGCTAAACAAGGACTGA+AGG 0.585400 2_1:+75050141 Msa0225000:CDS
CGATTTGTCCTGAACACCAG+CGG 0.594621 2_1:-75050811 None:intergenic
AGTATGGATCAGAGGACTGG+GGG 0.620757 2_1:+75050878 Msa0225000:CDS
ATTCAAGCAGTATGGATCAG+AGG 0.622044 2_1:+75050870 Msa0225000:CDS
TTGGAGAATGATGCTAAACA+AGG 0.654196 2_1:+75050133 Msa0225000:CDS
CAGTATGGATCAGAGGACTG+GGG 0.660986 2_1:+75050877 Msa0225000:CDS
GTATGGATCAGAGGACTGGG+GGG 0.685351 2_1:+75050879 Msa0225000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATACAAATGTAATTCA+TGG - chr2_1:75050362-75050381 None:intergenic 15.0%
!! AAATATACAAATGTAATTCA+TGG - chr2_1:75050362-75050381 None:intergenic 15.0%
!! TGAAGGTAAATTAAATTTAG+CGG + chr2_1:75050158-75050177 Msa0225000:intron 20.0%
!!! AAAGTAATCATATTGATAGT+AGG - chr2_1:75050240-75050259 None:intergenic 20.0%
!! TACTATCAATATGATTACTT+TGG + chr2_1:75050239-75050258 Msa0225000:intron 20.0%
!! CAGATAACAAAATGAAATTA+TGG - chr2_1:75050303-75050322 None:intergenic 20.0%
!!! TTTCCAGCTTTAAATTATAA+AGG + chr2_1:75050444-75050463 Msa0225000:intron 20.0%
!!! AAACCTTTATAATTTAAAGC+TGG - chr2_1:75050450-75050469 None:intergenic 20.0%
!!! GCTTTAAATTATAAAGGTTT+TGG + chr2_1:75050450-75050469 Msa0225000:intron 20.0%
!! GTCTAAAAGAAAAGATAAAA+GGG - chr2_1:75050701-75050720 None:intergenic 20.0%
!! AGTCTAAAAGAAAAGATAAA+AGG - chr2_1:75050702-75050721 None:intergenic 20.0%
!!! AACAATGTTTTAAAAAGCAA+GGG - chr2_1:75050760-75050779 None:intergenic 20.0%
!!! TAACAATGTTTTAAAAAGCA+AGG - chr2_1:75050761-75050780 None:intergenic 20.0%
!! TGAAGGTAAATTAAATTTAG+CGG + chr2_1:75050158-75050177 Msa0225000:intron 20.0%
!!! AAAGTAATCATATTGATAGT+AGG - chr2_1:75050240-75050259 None:intergenic 20.0%
!! TACTATCAATATGATTACTT+TGG + chr2_1:75050239-75050258 Msa0225000:intron 20.0%
!! CAGATAACAAAATGAAATTA+TGG - chr2_1:75050303-75050322 None:intergenic 20.0%
!!! TTTCCAGCTTTAAATTATAA+AGG + chr2_1:75050444-75050463 Msa0225000:intron 20.0%
!!! AAACCTTTATAATTTAAAGC+TGG - chr2_1:75050450-75050469 None:intergenic 20.0%
!!! GCTTTAAATTATAAAGGTTT+TGG + chr2_1:75050450-75050469 Msa0225000:intron 20.0%
!! GTCTAAAAGAAAAGATAAAA+GGG - chr2_1:75050701-75050720 None:intergenic 20.0%
!! AGTCTAAAAGAAAAGATAAA+AGG - chr2_1:75050702-75050721 None:intergenic 20.0%
!!! AACAATGTTTTAAAAAGCAA+GGG - chr2_1:75050760-75050779 None:intergenic 20.0%
!!! TAACAATGTTTTAAAAAGCA+AGG - chr2_1:75050761-75050780 None:intergenic 20.0%
! TAGTATGTATAATCAAAACC+AGG - chr2_1:75050043-75050062 None:intergenic 25.0%
!! AATATGATTACTTTGGTTCA+AGG + chr2_1:75050246-75050265 Msa0225000:intron 25.0%
!! TATAATTTAAAGCTGGAAAG+AGG - chr2_1:75050443-75050462 None:intergenic 25.0%
! ACGAGAAAAAAAACTAAAGA+AGG + chr2_1:75050498-75050517 Msa0225000:intron 25.0%
!!! AAAGTACAGTCTTATTATGA+AGG - chr2_1:75050560-75050579 None:intergenic 25.0%
!! AGATTTTCATTTGATGAGAA+TGG + chr2_1:75050934-75050953 Msa0225000:CDS 25.0%
! TAGTATGTATAATCAAAACC+AGG - chr2_1:75050043-75050062 None:intergenic 25.0%
!! AATATGATTACTTTGGTTCA+AGG + chr2_1:75050246-75050265 Msa0225000:intron 25.0%
!! TATAATTTAAAGCTGGAAAG+AGG - chr2_1:75050443-75050462 None:intergenic 25.0%
! ACGAGAAAAAAAACTAAAGA+AGG + chr2_1:75050498-75050517 Msa0225000:intron 25.0%
!!! AAAGTACAGTCTTATTATGA+AGG - chr2_1:75050560-75050579 None:intergenic 25.0%
!! AGATTTTCATTTGATGAGAA+TGG + chr2_1:75050934-75050953 Msa0225000:CDS 25.0%
ATTGATGCATATGTTAAAGC+TGG + chr2_1:75050103-75050122 Msa0225000:CDS 30.0%
GTAATTCATGGTCAAAACAT+GGG - chr2_1:75050350-75050369 None:intergenic 30.0%
TGTAATTCATGGTCAAAACA+TGG - chr2_1:75050351-75050370 None:intergenic 30.0%
TGCATAAGTAACATTGATAC+TGG - chr2_1:75050396-75050415 None:intergenic 30.0%
!! ATGCATTATTTCTAGCAGAA+TGG + chr2_1:75050411-75050430 Msa0225000:intron 30.0%
!! ACAGTCTTATTATGAAGGTA+AGG - chr2_1:75050555-75050574 None:intergenic 30.0%
TTATACTTTGCTTGTAGGTT+TGG + chr2_1:75050590-75050609 Msa0225000:intron 30.0%
ATTGATGCATATGTTAAAGC+TGG + chr2_1:75050103-75050122 Msa0225000:CDS 30.0%
GTAATTCATGGTCAAAACAT+GGG - chr2_1:75050350-75050369 None:intergenic 30.0%
TGTAATTCATGGTCAAAACA+TGG - chr2_1:75050351-75050370 None:intergenic 30.0%
TGCATAAGTAACATTGATAC+TGG - chr2_1:75050396-75050415 None:intergenic 30.0%
!! ATGCATTATTTCTAGCAGAA+TGG + chr2_1:75050411-75050430 Msa0225000:intron 30.0%
!! ACAGTCTTATTATGAAGGTA+AGG - chr2_1:75050555-75050574 None:intergenic 30.0%
TTATACTTTGCTTGTAGGTT+TGG + chr2_1:75050590-75050609 Msa0225000:intron 30.0%
TTGGAGAATGATGCTAAACA+AGG + chr2_1:75050133-75050152 Msa0225000:CDS 35.0%
GAAGGTTATCATCTTCTTTG+AGG + chr2_1:75050516-75050535 Msa0225000:intron 35.0%
AAGCAAAGTATAACAGCATG+GGG - chr2_1:75050583-75050602 None:intergenic 35.0%
CAAGCAAAGTATAACAGCAT+GGG - chr2_1:75050584-75050603 None:intergenic 35.0%
ACAAGCAAAGTATAACAGCA+TGG - chr2_1:75050585-75050604 None:intergenic 35.0%
TGCTGTTATACTTTGCTTGT+AGG + chr2_1:75050585-75050604 Msa0225000:intron 35.0%
CGAAACATATTCAAGCAGTA+TGG + chr2_1:75050862-75050881 Msa0225000:CDS 35.0%
!! CCTGCATATTTTCAGGAAAA+GGG - chr2_1:75050984-75051003 None:intergenic 35.0%
!! ACCTGCATATTTTCAGGAAA+AGG - chr2_1:75050985-75051004 None:intergenic 35.0%
TTGGAGAATGATGCTAAACA+AGG + chr2_1:75050133-75050152 Msa0225000:CDS 35.0%
GAAGGTTATCATCTTCTTTG+AGG + chr2_1:75050516-75050535 Msa0225000:intron 35.0%
AAGCAAAGTATAACAGCATG+GGG - chr2_1:75050583-75050602 None:intergenic 35.0%
CAAGCAAAGTATAACAGCAT+GGG - chr2_1:75050584-75050603 None:intergenic 35.0%
ACAAGCAAAGTATAACAGCA+TGG - chr2_1:75050585-75050604 None:intergenic 35.0%
TGCTGTTATACTTTGCTTGT+AGG + chr2_1:75050585-75050604 Msa0225000:intron 35.0%
CGAAACATATTCAAGCAGTA+TGG + chr2_1:75050862-75050881 Msa0225000:CDS 35.0%
!! CCTGCATATTTTCAGGAAAA+GGG - chr2_1:75050984-75051003 None:intergenic 35.0%
!! ACCTGCATATTTTCAGGAAA+AGG - chr2_1:75050985-75051004 None:intergenic 35.0%
TGTTAAAGCTGGACTTGTCT+TGG + chr2_1:75050114-75050133 Msa0225000:CDS 40.0%
TGATGCTAAACAAGGACTGA+AGG + chr2_1:75050141-75050160 Msa0225000:CDS 40.0%
GAAGGTAAGGTTTCCTATCA+GGG - chr2_1:75050542-75050561 None:intergenic 40.0%
TGAAGGTAAGGTTTCCTATC+AGG - chr2_1:75050543-75050562 None:intergenic 40.0%
ATGCCTTGCATTCATCAAAG+TGG - chr2_1:75050661-75050680 None:intergenic 40.0%
CTTTGATGAATGCAAGGCAT+AGG + chr2_1:75050661-75050680 Msa0225000:intron 40.0%
TGAATGCAAGGCATAGGAAA+AGG + chr2_1:75050667-75050686 Msa0225000:intron 40.0%
ATGTTCAGCAGCAAAAGCTA+AGG - chr2_1:75050734-75050753 None:intergenic 40.0%
AAATAGCTGATATAAGCCGC+TGG + chr2_1:75050795-75050814 Msa0225000:intron 40.0%
ATTCAAGCAGTATGGATCAG+AGG + chr2_1:75050870-75050889 Msa0225000:CDS 40.0%
! CCCTTTTCCTGAAAATATGC+AGG + chr2_1:75050981-75051000 Msa0225000:CDS 40.0%
! CACTCTACCTGCATATTTTC+AGG - chr2_1:75050991-75051010 None:intergenic 40.0%
TGTTAAAGCTGGACTTGTCT+TGG + chr2_1:75050114-75050133 Msa0225000:CDS 40.0%
TGATGCTAAACAAGGACTGA+AGG + chr2_1:75050141-75050160 Msa0225000:CDS 40.0%
GAAGGTAAGGTTTCCTATCA+GGG - chr2_1:75050542-75050561 None:intergenic 40.0%
TGAAGGTAAGGTTTCCTATC+AGG - chr2_1:75050543-75050562 None:intergenic 40.0%
ATGCCTTGCATTCATCAAAG+TGG - chr2_1:75050661-75050680 None:intergenic 40.0%
CTTTGATGAATGCAAGGCAT+AGG + chr2_1:75050661-75050680 Msa0225000:intron 40.0%
TGAATGCAAGGCATAGGAAA+AGG + chr2_1:75050667-75050686 Msa0225000:intron 40.0%
ATGTTCAGCAGCAAAAGCTA+AGG - chr2_1:75050734-75050753 None:intergenic 40.0%
AAATAGCTGATATAAGCCGC+TGG + chr2_1:75050795-75050814 Msa0225000:intron 40.0%
ATTCAAGCAGTATGGATCAG+AGG + chr2_1:75050870-75050889 Msa0225000:CDS 40.0%
! CCCTTTTCCTGAAAATATGC+AGG + chr2_1:75050981-75051000 Msa0225000:CDS 40.0%
! CACTCTACCTGCATATTTTC+AGG - chr2_1:75050991-75051010 None:intergenic 40.0%
TATGGATCTGTCATCGTACC+TGG + chr2_1:75050022-75050041 Msa0225000:intron 45.0%
!! ATCTTCTTTGAGGCCCTGAT+AGG + chr2_1:75050526-75050545 Msa0225000:intron 45.0%
! ACGCCACTTTGATGAATGCA+AGG + chr2_1:75050655-75050674 Msa0225000:intron 45.0%
TATGGATCTGTCATCGTACC+TGG + chr2_1:75050022-75050041 Msa0225000:intron 45.0%
!! ATCTTCTTTGAGGCCCTGAT+AGG + chr2_1:75050526-75050545 Msa0225000:intron 45.0%
! ACGCCACTTTGATGAATGCA+AGG + chr2_1:75050655-75050674 Msa0225000:intron 45.0%
!!! AAAGTTTTTTTTTTTTTTTT+TGG + chr2_1:75049978-75049997 Msa0225000:intron 5.0%
!!! AAGTTTTTTTTTTTTTTTTT+GGG + chr2_1:75049979-75049998 Msa0225000:intron 5.0%
!! TTATAAATTACAAATAATTA+TGG + chr2_1:75050004-75050023 Msa0225000:intron 5.0%
!!! AAAGTTTTTTTTTTTTTTTT+TGG + chr2_1:75049978-75049997 Msa0225000:intron 5.0%
!!! AAGTTTTTTTTTTTTTTTTT+GGG + chr2_1:75049979-75049998 Msa0225000:intron 5.0%
!! TTATAAATTACAAATAATTA+TGG + chr2_1:75050004-75050023 Msa0225000:intron 5.0%
GATATAAGCCGCTGGTGTTC+AGG + chr2_1:75050803-75050822 Msa0225000:intron 50.0%
CGATTTGTCCTGAACACCAG+CGG - chr2_1:75050814-75050833 None:intergenic 50.0%
CGCTGGTGTTCAGGACAAAT+CGG + chr2_1:75050812-75050831 Msa0225000:CDS 50.0%
AGCAGTATGGATCAGAGGAC+TGG + chr2_1:75050875-75050894 Msa0225000:CDS 50.0%
GCAGTATGGATCAGAGGACT+GGG + chr2_1:75050876-75050895 Msa0225000:CDS 50.0%
CAGTATGGATCAGAGGACTG+GGG + chr2_1:75050877-75050896 Msa0225000:CDS 50.0%
AGTATGGATCAGAGGACTGG+GGG + chr2_1:75050878-75050897 Msa0225000:CDS 50.0%
GATATAAGCCGCTGGTGTTC+AGG + chr2_1:75050803-75050822 Msa0225000:intron 50.0%
CGATTTGTCCTGAACACCAG+CGG - chr2_1:75050814-75050833 None:intergenic 50.0%
CGCTGGTGTTCAGGACAAAT+CGG + chr2_1:75050812-75050831 Msa0225000:CDS 50.0%
AGCAGTATGGATCAGAGGAC+TGG + chr2_1:75050875-75050894 Msa0225000:CDS 50.0%
GCAGTATGGATCAGAGGACT+GGG + chr2_1:75050876-75050895 Msa0225000:CDS 50.0%
CAGTATGGATCAGAGGACTG+GGG + chr2_1:75050877-75050896 Msa0225000:CDS 50.0%
AGTATGGATCAGAGGACTGG+GGG + chr2_1:75050878-75050897 Msa0225000:CDS 50.0%
GTATGGATCAGAGGACTGGG+GGG + chr2_1:75050879-75050898 Msa0225000:CDS 55.0%
GTATGGATCAGAGGACTGGG+GGG + chr2_1:75050879-75050898 Msa0225000:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75049953 75051011 75049953 ID=Msa0225000;Name=Msa0225000
chr2_1 mRNA 75049953 75051011 75049953 ID=Msa0225000-mRNA-1;Parent=Msa0225000;Name=Msa0225000-mRNA-1;_AED=0.50;_eAED=0.57;_QI=0|0|0|0.66|1|1|3|0|91
chr2_1 exon 75049953 75049959 75049953 ID=Msa0225000-mRNA-1:exon:22401;Parent=Msa0225000-mRNA-1
chr2_1 exon 75050095 75050162 75050095 ID=Msa0225000-mRNA-1:exon:22402;Parent=Msa0225000-mRNA-1
chr2_1 exon 75050811 75051011 75050811 ID=Msa0225000-mRNA-1:exon:22403;Parent=Msa0225000-mRNA-1
chr2_1 CDS 75049953 75049959 75049953 ID=Msa0225000-mRNA-1:cds;Parent=Msa0225000-mRNA-1
chr2_1 CDS 75050095 75050162 75050095 ID=Msa0225000-mRNA-1:cds;Parent=Msa0225000-mRNA-1
chr2_1 CDS 75050811 75051011 75050811 ID=Msa0225000-mRNA-1:cds;Parent=Msa0225000-mRNA-1
Gene Sequence

>Msa0225000

ATGAATAAAGTCATAATTGATGCATATGTTAAAGCTGGACTTGTCTTGGAGAATGATGCTAAACAAGGACTGAAGCCGCTGGTGTTCAGGACAAATCGGAAGAGAAATGATGTCTCTTTTGATGCACGAAACATATTCAAGCAGTATGGATCAGAGGACTGGGGGGAATATCTTATTCGTGAAGCTCATCTATCGAAGAGATTTTCATTTGATGAGAATGGATACTATCATCGCTGTGCTTCAATCCCTTTTCCTGAAAATATGCAGGTAGAGTGA

Protein sequence

>Msa0225000

MNKVIIDAYVKAGLVLENDAKQGLKPLVFRTNRKRNDVSFDARNIFKQYGSEDWGEYLIREAHLSKRFSFDENGYYHRCASIPFPENMQVE*