AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1311960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1311960 MtrunA17_Chr8g0340131 92.340 235 17 1 1 235 1 234 7.75e-125 353
Msa1311960 MtrunA17_Chr7g0236051 48.214 112 48 2 124 235 238 339 7.51e-28 108
Msa1311960 MtrunA17_Chr4g0075411 50.420 119 51 3 123 235 101 217 1.73e-27 104
Msa1311960 MtrunA17_Chr2g0289321 46.610 118 37 3 118 235 122 213 2.80e-26 101
Msa1311960 MtrunA17_Chr3g0110201 47.748 111 51 2 125 235 249 352 5.18e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1311960 AT3G25030.3 35.537 242 108 7 3 235 8 210 8.25e-37 129
Msa1311960 AT3G25030.1 35.659 258 126 8 3 235 8 250 5.54e-36 128
Msa1311960 AT3G25030.4 35.659 258 126 8 3 235 8 250 5.54e-36 128
Msa1311960 AT3G25030.2 35.659 258 126 8 3 235 8 250 5.54e-36 128
Msa1311960 AT4G13100.5 31.673 281 112 6 3 235 8 256 4.86e-34 123
Msa1311960 AT4G13100.3 31.673 281 112 6 3 235 8 256 4.86e-34 123
Msa1311960 AT4G13100.4 31.338 284 112 7 3 235 5 256 9.34e-33 119
Msa1311960 AT4G13100.8 31.338 284 112 7 3 235 8 259 1.00e-32 119
Msa1311960 AT4G13100.7 31.338 284 112 7 3 235 8 259 1.00e-32 119
Msa1311960 AT4G13100.9 31.338 284 112 7 3 235 8 259 1.00e-32 119
Msa1311960 AT4G13100.1 31.338 284 112 7 3 235 8 259 1.00e-32 119
Msa1311960 AT4G13100.2 50.820 122 42 2 118 235 158 265 1.66e-31 116
Msa1311960 AT4G03965.1 56.627 83 33 1 156 235 127 209 5.19e-27 103
Msa1311960 AT4G22250.1 58.025 81 33 1 156 235 134 214 3.07e-26 101
Msa1311960 AT1G62370.1 71.429 49 14 0 187 235 156 204 1.66e-23 94.4
Msa1311960 AT3G07120.1 47.222 108 51 2 129 235 258 360 3.06e-22 94.0

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGAAAACGGCGTCGTTT+TGG 0.283292 8_4:-8873912 None:intergenic
GATCCGACGGAATTTCTTCC+CGG 0.365380 8_4:+8874162 Msa1311960:CDS
GGAAGAGAGTGAAATTGAAT+TGG 0.374216 8_4:+8874239 Msa1311960:CDS
ACATTAACAACAACGTTGTT+TGG 0.375493 8_4:-8873985 None:intergenic
GAATGTGTTCAAGAGAGCTT+TGG 0.390646 8_4:+8874454 Msa1311960:CDS
GCTGTTATTGTTGTTGTTGT+CGG 0.397472 8_4:-8874016 None:intergenic
CGTAAAGTGTTATTCTTTGA+CGG 0.401030 8_4:-8873840 None:intergenic
GTATAATATCTAAGAGTGTT+TGG 0.411313 8_4:-8873951 None:intergenic
GAAGAAGAGGAAAGAGAAGA+AGG 0.412992 8_4:+8874336 Msa1311960:CDS
AAATTCCGTCGGATCTGAAT+TGG 0.419938 8_4:-8874154 None:intergenic
CATGTCGTTGATGGATTTGT+TGG 0.421193 8_4:+8874218 Msa1311960:CDS
GTGTGTATGGTGAGGGATAA+AGG 0.427186 8_4:+8874396 Msa1311960:CDS
AGGAGAAGGGAGTAATGTTA+AGG 0.433410 8_4:+8874356 Msa1311960:CDS
AAAATCATGGAAAGCTTTCA+AGG 0.440126 8_4:+8874059 Msa1311960:CDS
AATGTGTTCAAGAGAGCTTT+GGG 0.444478 8_4:+8874455 Msa1311960:CDS
GCAGGTTAGCATGTCGTTGA+TGG 0.473676 8_4:+8874209 Msa1311960:CDS
AGGAAAGAGAAGAAGGAGAA+GGG 0.480424 8_4:+8874343 Msa1311960:CDS
GAGGAAAGAGAAGAAGGAGA+AGG 0.500274 8_4:+8874342 Msa1311960:CDS
ACACAATTGTTGCGTGTGTA+TGG 0.501642 8_4:+8874383 Msa1311960:CDS
TTGTTAAAGATCGAAAATCA+TGG 0.528304 8_4:+8874046 Msa1311960:CDS
GCTAACCTGCGCCGTCTCAT+CGG 0.529102 8_4:-8874196 None:intergenic
AGAGAGCTTTGGGTTAGTAG+AGG 0.548126 8_4:+8874465 Msa1311960:CDS
TCATCGGAAAACTCGCCGCC+GGG 0.552591 8_4:-8874180 None:intergenic
AGAAGGGAGTAATGTTAAGG+TGG 0.555525 8_4:+8874359 Msa1311960:CDS
CTCATCGGAAAACTCGCCGC+CGG 0.561315 8_4:-8874181 None:intergenic
CTGCAAAACGTGTGTCCACA+TGG 0.562500 8_4:-8874432 None:intergenic
TGAACTTCTAGTGTTGCTTG+TGG 0.565142 8_4:-8873875 None:intergenic
AGTAGTGACGTTGGTGATGA+AGG 0.567789 8_4:+8874267 Msa1311960:CDS
AAAATAAAATGATTGCAAAG+AGG 0.605793 8_4:-8874495 None:intergenic
TCCGACCAATTCAGATCCGA+CGG 0.610865 8_4:+8874149 Msa1311960:CDS
TTGTTGCGTGTGTATGGTGA+GGG 0.665548 8_4:+8874389 Msa1311960:CDS
CCGCCGGGAAGAAATTCCGT+CGG 0.673552 8_4:-8874165 None:intergenic
TCCGTCGGATCTGAATTGGT+CGG 0.675352 8_4:-8874150 None:intergenic
ATTGTTGCGTGTGTATGGTG+AGG 0.686096 8_4:+8874388 Msa1311960:CDS
GGTGCAGCGTTTATACCATG+TGG 0.688981 8_4:+8874417 Msa1311960:CDS
TCACCAACAACAACAAAACG+AGG 0.705279 8_4:+8874122 Msa1311960:CDS
TGGTGATGAAGGTGATGACG+TGG 0.717305 8_4:+8874278 Msa1311960:CDS
TTGGAACGAAGTAGTGACGT+TGG 0.731633 8_4:+8874258 Msa1311960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATGATTGCAAAG+AGG - chr8_4:8874498-8874517 None:intergenic 20.0%
!!! GTATAATATCTAAGAGTGTT+TGG - chr8_4:8873954-8873973 None:intergenic 25.0%
! TTGTTAAAGATCGAAAATCA+TGG + chr8_4:8874046-8874065 Msa1311960:CDS 25.0%
! CGTAAAGTGTTATTCTTTGA+CGG - chr8_4:8873843-8873862 None:intergenic 30.0%
!!! GTTTTTTTGGTTGGAGAAAA+CGG - chr8_4:8873926-8873945 None:intergenic 30.0%
!!! TTGGTTTGTGTTTTTTTGGT+TGG - chr8_4:8873935-8873954 None:intergenic 30.0%
!!! GTGTTTGGTTTGTGTTTTTT+TGG - chr8_4:8873939-8873958 None:intergenic 30.0%
ACATTAACAACAACGTTGTT+TGG - chr8_4:8873988-8874007 None:intergenic 30.0%
AAAATCATGGAAAGCTTTCA+AGG + chr8_4:8874059-8874078 Msa1311960:CDS 30.0%
AGAAGAAGAAAAAGAAGAAG+AGG + chr8_4:8874323-8874342 Msa1311960:CDS 30.0%
! GCTGTTATTGTTGTTGTTGT+CGG - chr8_4:8874019-8874038 None:intergenic 35.0%
!!! TATCCTCGTTTTGTTGTTGT+TGG - chr8_4:8874128-8874147 None:intergenic 35.0%
GGAAGAGAGTGAAATTGAAT+TGG + chr8_4:8874239-8874258 Msa1311960:CDS 35.0%
AATGTGTTCAAGAGAGCTTT+GGG + chr8_4:8874455-8874474 Msa1311960:CDS 35.0%
!! TGAACTTCTAGTGTTGCTTG+TGG - chr8_4:8873878-8873897 None:intergenic 40.0%
TCACCAACAACAACAAAACG+AGG + chr8_4:8874122-8874141 Msa1311960:CDS 40.0%
AAATTCCGTCGGATCTGAAT+TGG - chr8_4:8874157-8874176 None:intergenic 40.0%
!! CATGTCGTTGATGGATTTGT+TGG + chr8_4:8874218-8874237 Msa1311960:CDS 40.0%
GAAGAAGAGGAAAGAGAAGA+AGG + chr8_4:8874336-8874355 Msa1311960:CDS 40.0%
AGGAAAGAGAAGAAGGAGAA+GGG + chr8_4:8874343-8874362 Msa1311960:CDS 40.0%
AGGAGAAGGGAGTAATGTTA+AGG + chr8_4:8874356-8874375 Msa1311960:CDS 40.0%
AGAAGGGAGTAATGTTAAGG+TGG + chr8_4:8874359-8874378 Msa1311960:CDS 40.0%
ACACAATTGTTGCGTGTGTA+TGG + chr8_4:8874383-8874402 Msa1311960:CDS 40.0%
GAATGTGTTCAAGAGAGCTT+TGG + chr8_4:8874454-8874473 Msa1311960:CDS 40.0%
TTGGAACGAAGTAGTGACGT+TGG + chr8_4:8874258-8874277 Msa1311960:CDS 45.0%
!! AGTAGTGACGTTGGTGATGA+AGG + chr8_4:8874267-8874286 Msa1311960:CDS 45.0%
GAGGAAAGAGAAGAAGGAGA+AGG + chr8_4:8874342-8874361 Msa1311960:CDS 45.0%
ATTGTTGCGTGTGTATGGTG+AGG + chr8_4:8874388-8874407 Msa1311960:CDS 45.0%
TTGTTGCGTGTGTATGGTGA+GGG + chr8_4:8874389-8874408 Msa1311960:CDS 45.0%
GTGTGTATGGTGAGGGATAA+AGG + chr8_4:8874396-8874415 Msa1311960:CDS 45.0%
!! AGAGAGCTTTGGGTTAGTAG+AGG + chr8_4:8874465-8874484 Msa1311960:CDS 45.0%
! TGGAGAAAACGGCGTCGTTT+TGG - chr8_4:8873915-8873934 None:intergenic 50.0%
TCCGACCAATTCAGATCCGA+CGG + chr8_4:8874149-8874168 Msa1311960:CDS 50.0%
TCCGTCGGATCTGAATTGGT+CGG - chr8_4:8874153-8874172 None:intergenic 50.0%
GATCCGACGGAATTTCTTCC+CGG + chr8_4:8874162-8874181 Msa1311960:CDS 50.0%
GCAGGTTAGCATGTCGTTGA+TGG + chr8_4:8874209-8874228 Msa1311960:CDS 50.0%
! TGGTGATGAAGGTGATGACG+TGG + chr8_4:8874278-8874297 Msa1311960:CDS 50.0%
GGTGCAGCGTTTATACCATG+TGG + chr8_4:8874417-8874436 Msa1311960:CDS 50.0%
CTGCAAAACGTGTGTCCACA+TGG - chr8_4:8874435-8874454 None:intergenic 50.0%
! CGGCGAGTTTTCCGATGAGA+CGG + chr8_4:8874185-8874204 Msa1311960:CDS 55.0%
CCGCCGGGAAGAAATTCCGT+CGG - chr8_4:8874168-8874187 None:intergenic 60.0%
CCGACGGAATTTCTTCCCGG+CGG + chr8_4:8874165-8874184 Msa1311960:CDS 60.0%
TCATCGGAAAACTCGCCGCC+GGG - chr8_4:8874183-8874202 None:intergenic 60.0%
CTCATCGGAAAACTCGCCGC+CGG - chr8_4:8874184-8874203 None:intergenic 60.0%
! GTTTTCCGATGAGACGGCGC+AGG + chr8_4:8874191-8874210 Msa1311960:CDS 60.0%
GCTAACCTGCGCCGTCTCAT+CGG - chr8_4:8874199-8874218 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 8873832 8874539 8873832 ID=Msa1311960;Name=Msa1311960
chr8_4 mRNA 8873832 8874539 8873832 ID=Msa1311960-mRNA-1;Parent=Msa1311960;Name=Msa1311960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|235
chr8_4 exon 8873832 8874539 8873832 ID=Msa1311960-mRNA-1:exon:3257;Parent=Msa1311960-mRNA-1
chr8_4 CDS 8873832 8874539 8873832 ID=Msa1311960-mRNA-1:cds;Parent=Msa1311960-mRNA-1
Gene Sequence

>Msa1311960

ATGGAAAACCGTCAAAGAATAACACTTTACGATCAAATGACATCCACAAGCAACACTAGAAGTTCACTAGCTAGTTTAATCCAAAACGACGCCGTTTTCTCCAACCAAAAAAACACAAACCAAACACTCTTAGATATTATACGAGAAAACGAACCAAACAACGTTGTTGTTAATGTTAGTGTTACCGACAACAACAACAATAACAGCAACAACGTTGTTAAAGATCGAAAATCATGGAAAGCTTTCAAGGAATTGCTTCTTTTCAAACGTCGTAACTCTGAAGAATCAACTCACCAACAACAACAAAACGAGGATATTCCGACCAATTCAGATCCGACGGAATTTCTTCCCGGCGGCGAGTTTTCCGATGAGACGGCGCAGGTTAGCATGTCGTTGATGGATTTGTTGGAAGAGAGTGAAATTGAATTGGAACGAAGTAGTGACGTTGGTGATGAAGGTGATGACGTGGAAGAGAATAATGAGAAACGAGAAGAAGAAGAAAAAGAAGAAGAGGAAAGAGAAGAAGGAGAAGGGAGTAATGTTAAGGTGGAACACAATTGTTGCGTGTGTATGGTGAGGGATAAAGGTGCAGCGTTTATACCATGTGGACACACGTTTTGCAGAATGTGTTCAAGAGAGCTTTGGGTTAGTAGAGGAAATTGTCCTCTTTGCAATCATTTTATTTTAGAAGTTCTTGATATTTTCTGA

Protein sequence

>Msa1311960

MENRQRITLYDQMTSTSNTRSSLASLIQNDAVFSNQKNTNQTLLDIIRENEPNNVVVNVSVTDNNNNNSNNVVKDRKSWKAFKELLLFKRRNSEESTHQQQQNEDIPTNSDPTEFLPGGEFSDETAQVSMSLMDLLEESEIELERSSDVGDEGDDVEENNEKREEEEKEEEEREEGEGSNVKVEHNCCVCMVRDKGAAFIPCGHTFCRMCSRELWVSRGNCPLCNHFILEVLDIF*