AlfalfaGEDB | Msa0999700

M. sativa cultivar zhongmu-4 / Msa0999700

KEGG: -
GO: GO:0001558 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0008150 GO:0010769 GO:0017016 GO:0017048 GO:0019899 GO:0022603 GO:0022604 GO:0031267 GO:0040008 GO:0042802 GO:0043900 GO:0045595 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051128 GO:0051239 GO:0051510 GO:0060284 GO:0065007 GO:0065009 GO:0080092 GO:0098772 GO:2000241
PFAM: PF03759.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999700	XP_003620607.2	97.538	528	12	1	1	527	22	549	0.0	1061

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999700	sp\|Q9LV40\|ROGF8_ARATH	65.563	453	139	6	76	527	87	523	0.0	577

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999700	F6LPV1	97.538	528	12	1	1	527	22	549	0.0	1061

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0104500	Msa0999700	0.805170	1.519439e-49	-8.615850e-47
Msa0124130	Msa0999700	0.832429	9.499833e-56	-8.615850e-47
Msa0149140	Msa0999700	0.800718	1.263650e-48	-8.615850e-47
Msa0206800	Msa0999700	0.811569	6.557239e-51	-8.615850e-47
Msa0221290	Msa0999700	0.800241	1.580952e-48	-8.615850e-47
Msa0252770	Msa0999700	0.814298	1.654625e-51	-8.615850e-47
Msa0280750	Msa0999700	0.800055	1.724537e-48	-8.615850e-47
Msa0288200	Msa0999700	0.800235	1.585271e-48	-8.615850e-47
Msa0291330	Msa0999700	0.809144	2.187450e-50	-8.615850e-47
Msa0322830	Msa0999700	0.806585	7.657302e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999700	MtrunA17_Chr6g0485861	97.538	528	12	1	1	527	22	549	0.0	1061
Msa0999700	MtrunA17_Chr2g0331631	60.413	533	173	12	6	527	28	533	0.0	580
Msa0999700	MtrunA17_Chr4g0004771	64.579	463	143	11	74	527	90	540	0.0	572
Msa0999700	MtrunA17_Chr7g0240501	52.451	408	156	5	73	460	183	572	1.34e-142	425
Msa0999700	MtrunA17_Chr4g0008771	53.704	378	159	4	62	424	84	460	1.41e-135	404
Msa0999700	MtrunA17_Chr4g0038651	52.533	375	160	5	73	430	86	459	1.76e-131	394
Msa0999700	MtrunA17_Chr3g0112731	50.653	383	163	5	73	436	99	474	4.29e-130	391
Msa0999700	MtrunA17_Chr5g0436141	51.316	380	159	5	76	436	84	456	8.58e-130	390
Msa0999700	MtrunA17_Chr8g0348921	47.074	376	174	5	73	424	96	470	6.00e-119	358
Msa0999700	MtrunA17_Chr7g0247761	43.780	418	167	7	74	424	113	529	1.41e-109	336

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999700	AT3G24620.1	65.563	453	139	6	76	527	87	523	0.0	577
Msa0999700	AT4G13240.1	62.979	470	150	7	65	527	65	517	0.0	575
Msa0999700	AT1G79860.1	62.582	457	127	11	78	527	96	515	0.0	541
Msa0999700	AT1G52240.1	61.894	454	150	7	78	527	108	542	0.0	538
Msa0999700	AT5G19560.1	57.328	464	176	7	73	527	43	493	3.49e-174	501
Msa0999700	AT5G19560.5	57.328	464	176	7	73	527	43	493	3.49e-174	501
Msa0999700	AT5G19560.4	57.328	464	176	7	73	527	62	512	7.19e-174	500
Msa0999700	AT3G16130.1	50.962	520	213	11	19	525	44	534	3.23e-164	477
Msa0999700	AT5G19560.2	63.855	332	116	2	59	387	48	378	3.38e-148	430
Msa0999700	AT5G02010.1	48.798	416	189	6	73	465	69	483	1.01e-137	409

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Find 111 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAAGATGAATAAAGA+TGG	0.515789	6_4:-82035966	Msa0999700:CDS
AAAAGTGATGAGGAAGCTGA+AGG	0.607695	6_4:-82036525	Msa0999700:CDS
AAACCTTGAATATGTTTCCC+AGG	0.494123	6_4:+82038594	None:intergenic
AAAGAAAGAGTGACAAATGG+TGG	0.626399	6_4:-82036488	Msa0999700:CDS
AAATCTGAGAGAGTCATTGA+TGG	0.588625	6_4:+82035181	None:intergenic
AAATGGTGTTTCTTCAGCAC+TGG	0.456274	6_4:-82037830	Msa0999700:CDS
AACCTTGAATATGTTTCCCA+GGG	0.559119	6_4:+82038595	None:intergenic
AACTCTGAGGATGGTGGGAT+AGG	0.517108	6_4:-82035229	Msa0999700:CDS
AAGAATCCAACGAGAATCTC+CGG	0.517591	6_4:+82035278	None:intergenic
AAGCTGAGTGTTTACCTCCA+TGG	0.443142	6_4:+82037271	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGATAAGGATCATGATGA+AGG	-	chr6_4:82035075-82035094	Msa0999700:CDS	30.0%
TTGATAAGGATCATGATGAA+GGG	-	chr6_4:82035076-82035095	Msa0999700:CDS	30.0%
ATATACCAGTGATATTCCAT+TGG	-	chr6_4:82035149-82035168	Msa0999700:CDS	30.0%
AGAAAAATTATACCAGTTGG+TGG	+	chr6_4:82035252-82035271	None:intergenic	30.0%
AAAGTCATGAACTAAGATCA+TGG	-	chr6_4:82035326-82035345	Msa0999700:CDS	30.0%
AAGTCATGAACTAAGATCAT+GGG	-	chr6_4:82035327-82035346	Msa0999700:CDS	30.0%
TTCCTACCATAAAGATTTGA+GGG	+	chr6_4:82035886-82035905	None:intergenic	30.0%
TTTCCTACCATAAAGATTTG+AGG	+	chr6_4:82035887-82035906	None:intergenic	30.0%
CTTTATGGTAGGAAATGAAA+TGG	-	chr6_4:82035892-82035911	Msa0999700:CDS	30.0%
AACTTTAATCGTAACTTCGT+AGG	-	chr6_4:82036055-82036074	Msa0999700:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	82035009	82038658	82035009	ID=Msa0999700;Name=Msa0999700
chr6_4	mRNA	82035009	82038658	82035009	ID=Msa0999700-mRNA-1;Parent=Msa0999700;Name=Msa0999700-mRNA-1;_AED=0.04;_eAED=0.04;_QI=30\|1\|0.71\|1\|1\|1\|7\|0\|527
chr6_4	exon	82035009	82035431	82035009	ID=Msa0999700-mRNA-1:exon:15716;Parent=Msa0999700-mRNA-1
chr6_4	exon	82035821	82036138	82035821	ID=Msa0999700-mRNA-1:exon:15715;Parent=Msa0999700-mRNA-1
chr6_4	exon	82036320	82036595	82036320	ID=Msa0999700-mRNA-1:exon:15714;Parent=Msa0999700-mRNA-1
chr6_4	exon	82036772	82036846	82036772	ID=Msa0999700-mRNA-1:exon:15713;Parent=Msa0999700-mRNA-1
chr6_4	exon	82037286	82037440	82037286	ID=Msa0999700-mRNA-1:exon:15712;Parent=Msa0999700-mRNA-1
chr6_4	exon	82037800	82037916	82037800	ID=Msa0999700-mRNA-1:exon:15711;Parent=Msa0999700-mRNA-1
chr6_4	exon	82038409	82038658	82038409	ID=Msa0999700-mRNA-1:exon:15710;Parent=Msa0999700-mRNA-1
chr6_4	five_prime_UTR	82038629	82038658	82038629	ID=Msa0999700-mRNA-1:five_prime_utr;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82038409	82038628	82038409	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82037800	82037916	82037800	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82037286	82037440	82037286	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82036772	82036846	82036772	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82036320	82036595	82036320	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82035821	82036138	82035821	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1
chr6_4	CDS	82035009	82035431	82035009	ID=Msa0999700-mRNA-1:cds;Parent=Msa0999700-mRNA-1

Gene Sequence

>Msa0999700

ATGTTTGAGATCCCTGGGAAACATATTCAAGGTTTATTTGATAAGGATCATGATGAAGGGAGTGATCATAGTGATCATAAAGTTTACTCAAAAAGCTTTGAATCAGCAAGATATACCAGTGATATTCCATTGGAAACTCAGCCAATAGGAGTTGCTCTCAATATTAATAATGATCATATTCAAGAAGCAGCAGCTAGAATGCCTCCTAAGCCACCAACTGAAACTGATTTGATGAAGGAAAGATTTGCTAAGCTTCTTCTAGGAGAAGACATGTCAGGTGCAGGAAATGGTGTTTCTTCAGCACTGGCTTTGTCAAATGCCATAACAAACCTTGCTGCATCTGTTTTTGGAGAACAATCAAAGTTGGAGCCAATGTCTCAAGAAAGGAAGACTAGGTGGAGAAAAGAAATTGAATGGCTTCTTTCTGTGACTGATCATATTGTTGAATTTGCTCCATCACAACAGTTAGCTAAGGATGGATCAACCATGGAGATCATGACGACTCGGCAAAGAAGCGATCTGCTCATGAACATTCCGGCCTTGCGCAAGCTTGATGCAATGCTCCTTGACATTTTGGACAACTTTAGAGATCAAAATGAATTCTGGTATGTCTCAAAAAGTGATGAGGAAGCTGAAGGTAATACTGTCACTCAAAGAAAGAGTGACAAATGGTGGCTCCCAGTTGTTAAAGTTCCTCCAACAGGGTTATCAGATGTAGCTGTGAAATGGATACAATTCCAGAAAGACAATGTTAACCAAGTACTTAAAGCAGCCATGGCAATAAATGCTCAAGTACTATCCGAGATGGAGATACCTGACAACTACATTGAATCTCTCCCTAAGAATGGTAGAGAAAGCCTTGGTGAGTCGATCTATAAGTGCATTACTGTGGAATACTTTGATCCGGGACAATTCCTCTCAACAATGGACATGACCACAGAACATAAAGTTCTTGACCTCAAGAATAGGATTGAAGCTTCTATTGTAATATGGAAAAGGAAGATGAATAAAGATGGAAAATCTTCATGGAGTTCGGCAATAAGCATGGAGAAGAGGGAACTCTTTGAAGAGAGAGCAGAAACAATACTGTTGATGATCAAACAAGAGTTTCCAGGACTTCCACAGTCTTCACTTGATATCAGCAAAATCCAATATAACAAGGATGTGGGACAAGCCATTCTAGAGAGCTATTCAAGAGTAATAGAAAGCTTGGCTTATACAGTTTTATCAAGGATTGATGATGTTTTATATGTGGATTCAATGACAAAGAATCCATCGTTGGCGGCATCCAGCCGGAGATTCTCGTTGGATTCTTTGCCGGTGGCTGATCAAACATCTCCAAACTCTGAGGATGGTGGGATAGGAAGGATGAGTTCTTCAGACACACCACCATCAATGACTCTCTCAGATTTCATGGGATGGACAAAAGGAGATTTGAAAAGGACTAATTCTACAGGTGACTTAGAAGACTTGAAAGACAAAGATGAAAAAGTTTTGAATAAGCTTGGAACACCGAAGAAAAATTATTACTTGGACAAGCTTGAGTATTTGAACACTATAAGAAGTCCTATCGCGCGACATTAA

Protein sequence

>Msa0999700

MFEIPGKHIQGLFDKDHDEGSDHSDHKVYSKSFESARYTSDIPLETQPIGVALNINNDHIQEAAARMPPKPPTETDLMKERFAKLLLGEDMSGAGNGVSSALALSNAITNLAASVFGEQSKLEPMSQERKTRWRKEIEWLLSVTDHIVEFAPSQQLAKDGSTMEIMTTRQRSDLLMNIPALRKLDAMLLDILDNFRDQNEFWYVSKSDEEAEGNTVTQRKSDKWWLPVVKVPPTGLSDVAVKWIQFQKDNVNQVLKAAMAINAQVLSEMEIPDNYIESLPKNGRESLGESIYKCITVEYFDPGQFLSTMDMTTEHKVLDLKNRIEASIVIWKRKMNKDGKSSWSSAISMEKRELFEERAETILLMIKQEFPGLPQSSLDISKIQYNKDVGQAILESYSRVIESLAYTVLSRIDDVLYVDSMTKNPSLAASSRRFSLDSLPVADQTSPNSEDGGIGRMSSSDTPPSMTLSDFMGWTKGDLKRTNSTGDLEDLKDKDEKVLNKLGTPKKNYYLDKLEYLNTIRSPIARH*