AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0964840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964840 MtrunA17_Chr6g0485481 50.376 133 34 2 28 128 1 133 4.38e-35 120
Msa0964840 MtrunA17_Chr6g0485491 66.667 66 22 0 85 150 50 115 6.61e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGAAAACATTGATTAT+TGG 0.176776 6_3:+91869774 Msa0964840:CDS
GCTCACCTTCTACTTCTTCT+TGG 0.223849 6_3:+91870575 Msa0964840:CDS
GGTCATTCTTCTTCATCTTC+TGG 0.226370 6_3:+91870553 Msa0964840:CDS
GACTGACTCTTCATCCCTTT+TGG 0.233722 6_3:-91870211 None:intergenic
ATAATATCATGTAGCGTTAA+TGG 0.280407 6_3:+91870532 Msa0964840:intron
ACTATTAAAGATCTCTTTGT+AGG 0.281391 6_3:-91869668 None:intergenic
AGAGTCAGTCAAAGGTTCTT+TGG 0.292750 6_3:+91870224 Msa0964840:CDS
TCAATTGAGATGGTTATGTT+AGG 0.316257 6_3:-91868174 None:intergenic
AGTATCACCTGGCTTTGATA+TGG 0.372210 6_3:-91869873 None:intergenic
GAGTCAGTCAAAGGTTCTTT+GGG 0.376370 6_3:+91870225 Msa0964840:CDS
AATTCATCATACGTTATTCC+AGG 0.389672 6_3:-91870291 None:intergenic
TTCTATTCACATCTTCCAAA+AGG 0.390556 6_3:+91870196 Msa0964840:CDS
CAATTGAGATGGTTATGTTA+GGG 0.394866 6_3:-91868173 None:intergenic
TCTTGACGCCGTCAGCCATT+TGG 0.395579 6_3:-91870110 None:intergenic
ACCTACACTCAACACATATT+CGG 0.429644 6_3:+91869709 Msa0964840:CDS
AGAAAAGGAGTGTTTGTTGT+TGG 0.434642 6_3:-91868144 None:intergenic
TTCATGATTGATGCTTGTTA+CGG 0.446394 6_3:+91870138 Msa0964840:CDS
ATTGATGCTTGTTACGGAGG+GGG 0.492034 6_3:+91870144 Msa0964840:CDS
ATTCACCAAGAAGAAGTAGA+AGG 0.495476 6_3:-91870580 None:intergenic
GAAGAACAACACTCAAAGCT+TGG 0.496080 6_3:-91868122 None:intergenic
TGATTGATGCTTGTTACGGA+GGG 0.498805 6_3:+91870142 Msa0964840:CDS
TCTATTCACATCTTCCAAAA+GGG 0.515999 6_3:+91870197 Msa0964840:CDS
AAGAACAACACTCAAAGCTT+GGG 0.524167 6_3:-91868121 None:intergenic
ATGTTAGGGGTGAAAAGAAA+AGG 0.530276 6_3:-91868159 None:intergenic
TTAATTACCATATCAAAGCC+AGG 0.542624 6_3:+91869866 Msa0964840:CDS
ATGATTGATGCTTGTTACGG+AGG 0.558998 6_3:+91870141 Msa0964840:CDS
ACTGTTTCTCTAGAGATGCT+AGG 0.561940 6_3:+91870482 Msa0964840:CDS
AATTGAGATGGTTATGTTAG+GGG 0.567185 6_3:-91868172 None:intergenic
TTGATGCTTGTTACGGAGGG+GGG 0.576592 6_3:+91870145 Msa0964840:CDS
GATTGATGCTTGTTACGGAG+GGG 0.589936 6_3:+91870143 Msa0964840:CDS
ATTCATCATACGTTATTCCA+GGG 0.594580 6_3:-91870290 None:intergenic
ATGTGAATAGAACAACTTTG+TGG 0.598030 6_3:-91870185 None:intergenic
AGGGATGAAGAGTCAGTCAA+AGG 0.601689 6_3:+91870216 Msa0964840:CDS
ATAGAATCAAGTTAAGAGAG+TGG 0.603141 6_3:+91870410 Msa0964840:intron
GATTGTGTTAATGATTACCC+TGG 0.604085 6_3:+91870273 Msa0964840:CDS
TCCGAATATGTGTTGAGTGT+AGG 0.619739 6_3:-91869710 None:intergenic
ACACTCAACACATATTCGGA+TGG 0.639597 6_3:+91869713 Msa0964840:CDS
TGAATAGAACAACTTTGTGG+TGG 0.649665 6_3:-91870182 None:intergenic
GTGTTAGACCAAATGGCTGA+CGG 0.654978 6_3:+91870102 Msa0964840:CDS
TCAGCATCAATAAGGAAACC+AGG 0.656233 6_3:-91869818 None:intergenic
GAATAGAACAACTTTGTGGT+GGG 0.689752 6_3:-91870181 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAAAGTTATATTTCTA+AGG - chr6_3:91868344-91868363 None:intergenic 10.0%
!! GATAAAAAAAATATATTACA+TGG + chr6_3:91869350-91869369 Msa0964840:intron 10.0%
!! TTACAAATAAAATAGAAAAA+TGG - chr6_3:91869599-91869618 None:intergenic 10.0%
!!! TGTATTTTATCAAGAAATTA+AGG + chr6_3:91868252-91868271 Msa0964840:CDS 15.0%
!! AAAATACATCTAAGAATATA+TGG - chr6_3:91868294-91868313 None:intergenic 15.0%
!! TAAAAATGATAATATCATGA+GGG - chr6_3:91869156-91869175 None:intergenic 15.0%
!! ATGATATGCAATAAAATAAA+TGG - chr6_3:91869515-91869534 None:intergenic 15.0%
!!! GTTTTTAATCACTATGTATT+TGG - chr6_3:91868218-91868237 None:intergenic 20.0%
!!! AAAATACATGCTTTTTTTCT+TGG - chr6_3:91868240-91868259 None:intergenic 20.0%
!!! TTATTATTTTGTAGTCCTTT+TGG + chr6_3:91868357-91868376 Msa0964840:intron 20.0%
!!! ATGCTTTTAATTTTAAACGT+GGG - chr6_3:91868400-91868419 None:intergenic 20.0%
!!! TATGCTTTTAATTTTAAACG+TGG - chr6_3:91868401-91868420 None:intergenic 20.0%
!! ATATTTGTTTATGTTGTGTT+TGG + chr6_3:91868995-91869014 Msa0964840:intron 20.0%
!!! TAAGTTTTCGAAATTATAAG+TGG + chr6_3:91869018-91869037 Msa0964840:intron 20.0%
!! CAATAAAAAATGTTACTACT+GGG + chr6_3:91869060-91869079 Msa0964840:intron 20.0%
!! CTAAAAATGATAATATCATG+AGG - chr6_3:91869157-91869176 None:intergenic 20.0%
!! CCTCAAATATGAATTATATT+AGG - chr6_3:91869299-91869318 None:intergenic 20.0%
!! CCTAATATAATTCATATTTG+AGG + chr6_3:91869296-91869315 Msa0964840:intron 20.0%
!! ATATGCAATAAAATAAATGG+CGG - chr6_3:91869512-91869531 None:intergenic 20.0%
!!! AAGATGAAAACATTGATTAT+TGG + chr6_3:91869774-91869793 Msa0964840:CDS 20.0%
!! TTTTAAACGTGGGAAAAAAA+TGG - chr6_3:91868390-91868409 None:intergenic 25.0%
! TATTAACTAAAAAAAAGGGC+TGG - chr6_3:91868658-91868677 None:intergenic 25.0%
!!! TTTTAAAGTAAAAAAAGGGC+TGG - chr6_3:91868707-91868726 None:intergenic 25.0%
! ATGATAGAAACATAACACAA+TGG - chr6_3:91868920-91868939 None:intergenic 25.0%
!!! GCGGTTTGTTAAAATAATTA+AGG - chr6_3:91868945-91868964 None:intergenic 25.0%
!!! CCAGTAGTAACATTTTTTAT+TGG - chr6_3:91869062-91869081 None:intergenic 25.0%
! CCAATAAAAAATGTTACTAC+TGG + chr6_3:91869059-91869078 Msa0964840:intron 25.0%
! ACTATTAAAGATCTCTTTGT+AGG - chr6_3:91869671-91869690 None:intergenic 25.0%
!! GAAAAAAATAAAGTATCACC+TGG - chr6_3:91869887-91869906 None:intergenic 25.0%
!!! CTTTATTTTTTTCACTTCAG+TGG + chr6_3:91869894-91869913 Msa0964840:intron 25.0%
!!! TAAAAAAGTGTTAGACCAAA+TGG + chr6_3:91870095-91870114 Msa0964840:CDS 25.0%
! CAAAGGATCCAAATATTATA+TGG + chr6_3:91870318-91870337 Msa0964840:intron 25.0%
!!! AGAATTTTTTATCTCATCAG+TGG - chr6_3:91870379-91870398 None:intergenic 25.0%
! ATAATATCATGTAGCGTTAA+TGG + chr6_3:91870532-91870551 Msa0964840:intron 25.0%
AATTGAGATGGTTATGTTAG+GGG - chr6_3:91868175-91868194 None:intergenic 30.0%
CAATTGAGATGGTTATGTTA+GGG - chr6_3:91868176-91868195 None:intergenic 30.0%
TCAATTGAGATGGTTATGTT+AGG - chr6_3:91868177-91868196 None:intergenic 30.0%
! GAGAAAGTTTTCAATTGAGA+TGG - chr6_3:91868187-91868206 None:intergenic 30.0%
CAACAAGACAAATCACATTT+AGG - chr6_3:91868460-91868479 None:intergenic 30.0%
! CTAAATGTGATTTGTCTTGT+TGG + chr6_3:91868458-91868477 Msa0964840:intron 30.0%
AAACATAAGATGATGAAGAG+AGG + chr6_3:91868783-91868802 Msa0964840:intron 30.0%
ACTAATCCGACAAATCTATA+CGG - chr6_3:91868874-91868893 None:intergenic 30.0%
! TTACTGATAGTGAAAATCGT+AGG + chr6_3:91869195-91869214 Msa0964840:intron 30.0%
! GTATTTTGTCAGCATCAATA+AGG - chr6_3:91869829-91869848 None:intergenic 30.0%
TTAATTACCATATCAAAGCC+AGG + chr6_3:91869866-91869885 Msa0964840:CDS 30.0%
ATGGTAACTATCAACAAAGA+GGG + chr6_3:91869919-91869938 Msa0964840:intron 30.0%
TATTTGTGATTCTCAATGCA+TGG + chr6_3:91870023-91870042 Msa0964840:intron 30.0%
! TTCATGATTGATGCTTGTTA+CGG + chr6_3:91870138-91870157 Msa0964840:CDS 30.0%
ATGTGAATAGAACAACTTTG+TGG - chr6_3:91870188-91870207 None:intergenic 30.0%
TTCTATTCACATCTTCCAAA+AGG + chr6_3:91870196-91870215 Msa0964840:CDS 30.0%
TCTATTCACATCTTCCAAAA+GGG + chr6_3:91870197-91870216 Msa0964840:CDS 30.0%
ATTCATCATACGTTATTCCA+GGG - chr6_3:91870293-91870312 None:intergenic 30.0%
AATTCATCATACGTTATTCC+AGG - chr6_3:91870294-91870313 None:intergenic 30.0%
CGTATGATGAATTGATACAA+AGG + chr6_3:91870301-91870320 Msa0964840:intron 30.0%
CATGCTGACCATATAATATT+TGG - chr6_3:91870329-91870348 None:intergenic 30.0%
CAAATATTATATGGTCAGCA+TGG + chr6_3:91870327-91870346 Msa0964840:intron 30.0%
ATAGAATCAAGTTAAGAGAG+TGG + chr6_3:91870410-91870429 Msa0964840:intron 30.0%
!! AGAAAAGGAGTGTTTGTTGT+TGG - chr6_3:91868147-91868166 None:intergenic 35.0%
ATGTTAGGGGTGAAAAGAAA+AGG - chr6_3:91868162-91868181 None:intergenic 35.0%
AAAAATGGAAAGGGACCAAA+AGG - chr6_3:91868375-91868394 None:intergenic 35.0%
ACGTGGGAAAAAAATGGAAA+GGG - chr6_3:91868384-91868403 None:intergenic 35.0%
AACGTGGGAAAAAAATGGAA+AGG - chr6_3:91868385-91868404 None:intergenic 35.0%
ATACACAACTATTGTGAGAG+TGG - chr6_3:91868967-91868986 None:intergenic 35.0%
ACCTACACTCAACACATATT+CGG + chr6_3:91869709-91869728 Msa0964840:CDS 35.0%
TATTGGCAAGACAAAAACGA+TGG + chr6_3:91869791-91869810 Msa0964840:CDS 35.0%
!!! TTTTTTCACTTCAGTGGACA+TGG + chr6_3:91869900-91869919 Msa0964840:intron 35.0%
CATGGTAACTATCAACAAAG+AGG + chr6_3:91869918-91869937 Msa0964840:intron 35.0%
TTGAGGAAGTTACCAAGTAA+AGG - chr6_3:91869975-91869994 None:intergenic 35.0%
TATTAGCATGAGATGTGTTG+AGG - chr6_3:91869992-91870011 None:intergenic 35.0%
GAATAGAACAACTTTGTGGT+GGG - chr6_3:91870184-91870203 None:intergenic 35.0%
TGAATAGAACAACTTTGTGG+TGG - chr6_3:91870185-91870204 None:intergenic 35.0%
GATTGTGTTAATGATTACCC+TGG + chr6_3:91870273-91870292 Msa0964840:CDS 35.0%
TTATCTCATCAGTGGCATAA+AGG - chr6_3:91870371-91870390 None:intergenic 35.0%
!!! TCTGCATCTAGCTTTTTCAT+TGG - chr6_3:91870455-91870474 None:intergenic 35.0%
TCTAGAGATGCTAGGTATTT+AGG + chr6_3:91870490-91870509 Msa0964840:intron 35.0%
CATGGGTGGAATTGGAAAAT+AGG + chr6_3:91868536-91868555 Msa0964840:intron 40.0%
! TGAGTTGAATCTACTCACCA+CGG + chr6_3:91868828-91868847 Msa0964840:intron 40.0%
TCGATGCCGTATAGATTTGT+CGG + chr6_3:91868865-91868884 Msa0964840:intron 40.0%
TGTTACTACTGGGTTCTTCT+AGG + chr6_3:91869070-91869089 Msa0964840:intron 40.0%
ATAGTGAAAATCGTAGGCAG+AGG + chr6_3:91869201-91869220 Msa0964840:intron 40.0%
TCCGAATATGTGTTGAGTGT+AGG - chr6_3:91869713-91869732 None:intergenic 40.0%
ACACTCAACACATATTCGGA+TGG + chr6_3:91869713-91869732 Msa0964840:CDS 40.0%
TCAGCATCAATAAGGAAACC+AGG - chr6_3:91869821-91869840 None:intergenic 40.0%
!! AGTATCACCTGGCTTTGATA+TGG - chr6_3:91869876-91869895 None:intergenic 40.0%
AATTACCTTGTTGCAGCCAA+TGG + chr6_3:91869945-91869964 Msa0964840:intron 40.0%
ACCAAGTAAAGGAGTTCCAT+TGG - chr6_3:91869964-91869983 None:intergenic 40.0%
! ATGATTGATGCTTGTTACGG+AGG + chr6_3:91870141-91870160 Msa0964840:CDS 40.0%
! TGATTGATGCTTGTTACGGA+GGG + chr6_3:91870142-91870161 Msa0964840:CDS 40.0%
AGAGTCAGTCAAAGGTTCTT+TGG + chr6_3:91870224-91870243 Msa0964840:CDS 40.0%
GAGTCAGTCAAAGGTTCTTT+GGG + chr6_3:91870225-91870244 Msa0964840:CDS 40.0%
! ACTGTTTCTCTAGAGATGCT+AGG + chr6_3:91870482-91870501 Msa0964840:CDS 40.0%
GGTCATTCTTCTTCATCTTC+TGG + chr6_3:91870553-91870572 Msa0964840:CDS 40.0%
!! CTATACGGCATCGATTCTGA+CGG - chr6_3:91868859-91868878 None:intergenic 45.0%
CACAACTATTGTGAGAGTGG+CGG - chr6_3:91868964-91868983 None:intergenic 45.0%
TGGCAAGACAAAAACGATGG+AGG + chr6_3:91869794-91869813 Msa0964840:CDS 45.0%
GCCAATGGAACTCCTTTACT+TGG + chr6_3:91869960-91869979 Msa0964840:intron 45.0%
! ATTTGCTACGTGACTTGTGC+AGG + chr6_3:91870063-91870082 Msa0964840:intron 45.0%
GTGTTAGACCAAATGGCTGA+CGG + chr6_3:91870102-91870121 Msa0964840:CDS 45.0%
! GATTGATGCTTGTTACGGAG+GGG + chr6_3:91870143-91870162 Msa0964840:CDS 45.0%
! ATTGATGCTTGTTACGGAGG+GGG + chr6_3:91870144-91870163 Msa0964840:CDS 45.0%
!! GACTGACTCTTCATCCCTTT+TGG - chr6_3:91870214-91870233 None:intergenic 45.0%
AGGGATGAAGAGTCAGTCAA+AGG + chr6_3:91870216-91870235 Msa0964840:CDS 45.0%
TCATCAGTGGCATAAAGGTC+GGG - chr6_3:91870366-91870385 None:intergenic 45.0%
CTCATCAGTGGCATAAAGGT+CGG - chr6_3:91870367-91870386 None:intergenic 45.0%
GCTCACCTTCTACTTCTTCT+TGG + chr6_3:91870575-91870594 Msa0964840:CDS 45.0%
!! TATTTATTAACTAAAAAAAA+GGG - chr6_3:91868662-91868681 None:intergenic 5.0%
!! TTATTTATTAACTAAAAAAA+AGG - chr6_3:91868663-91868682 None:intergenic 5.0%
!!! TTATTTTTAAAGTAAAAAAA+GGG - chr6_3:91868711-91868730 None:intergenic 5.0%
!!! ATTATTTTTAAAGTAAAAAA+AGG - chr6_3:91868712-91868731 None:intergenic 5.0%
!! TAAATTATTGTAAAAATAAA+TGG - chr6_3:91869108-91869127 None:intergenic 5.0%
!!! ATATATTTTTTTTATCTATA+TGG - chr6_3:91869346-91869365 None:intergenic 5.0%
!!! TAAATAATTAATAATTTGTA+AGG - chr6_3:91869423-91869442 None:intergenic 5.0%
ATGAACCTCACGCTGAAGTG+TGG + chr6_3:91868501-91868520 Msa0964840:intron 50.0%
TCCAATTCCACCCATGCCAA+AGG - chr6_3:91868532-91868551 None:intergenic 50.0%
GTTGAATCTACTCACCACGG+CGG + chr6_3:91868831-91868850 Msa0964840:intron 50.0%
GACAAAAACGATGGAGGACC+TGG + chr6_3:91869800-91869819 Msa0964840:CDS 50.0%
! GAGTTCCATTGGCTGCAACA+AGG - chr6_3:91869953-91869972 None:intergenic 50.0%
! TTGATGCTTGTTACGGAGGG+GGG + chr6_3:91870145-91870164 Msa0964840:CDS 50.0%
CAGCGTGAGGTTCATGCACA+TGG - chr6_3:91868496-91868515 None:intergenic 55.0%
CTGAAGTGTGGAGCTGCCTT+TGG + chr6_3:91868513-91868532 Msa0964840:intron 55.0%
TGTGGAGCTGCCTTTGGCAT+GGG + chr6_3:91868519-91868538 Msa0964840:intron 55.0%
GCCTTTGGCATGGGTGGAAT+TGG + chr6_3:91868528-91868547 Msa0964840:intron 55.0%
TCTTGACGCCGTCAGCCATT+TGG - chr6_3:91870113-91870132 None:intergenic 55.0%
GTGGCATAAAGGTCGGGTAG+TGG - chr6_3:91870360-91870379 None:intergenic 55.0%
CAGCTCCACACTTCAGCGTG+AGG - chr6_3:91868509-91868528 None:intergenic 60.0%
GTGTGGAGCTGCCTTTGGCA+TGG + chr6_3:91868518-91868537 Msa0964840:intron 60.0%
GGAGCTGCCTTTGGCATGGG+TGG + chr6_3:91868522-91868541 Msa0964840:intron 65.0%
CGATTCTGACGGTACCGCCG+TGG - chr6_3:91868848-91868867 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 91868123 91870600 91868123 ID=Msa0964840;Name=Msa0964840
chr6_3 mRNA 91868123 91870600 91868123 ID=Msa0964840-mRNA-1;Parent=Msa0964840;Name=Msa0964840-mRNA-1;_AED=0.23;_eAED=0.23;_QI=128|0|0.2|0.8|0.75|0.8|5|0|204
chr6_3 exon 91868123 91868273 91868123 ID=Msa0964840-mRNA-1:exon:16630;Parent=Msa0964840-mRNA-1
chr6_3 exon 91869658 91869887 91869658 ID=Msa0964840-mRNA-1:exon:16631;Parent=Msa0964840-mRNA-1
chr6_3 exon 91870085 91870301 91870085 ID=Msa0964840-mRNA-1:exon:16632;Parent=Msa0964840-mRNA-1
chr6_3 exon 91870414 91870503 91870414 ID=Msa0964840-mRNA-1:exon:16633;Parent=Msa0964840-mRNA-1
chr6_3 exon 91870546 91870600 91870546 ID=Msa0964840-mRNA-1:exon:16634;Parent=Msa0964840-mRNA-1
chr6_3 five_prime_UTR 91868123 91868250 91868123 ID=Msa0964840-mRNA-1:five_prime_utr;Parent=Msa0964840-mRNA-1
chr6_3 CDS 91868251 91868273 91868251 ID=Msa0964840-mRNA-1:cds;Parent=Msa0964840-mRNA-1
chr6_3 CDS 91869658 91869887 91869658 ID=Msa0964840-mRNA-1:cds;Parent=Msa0964840-mRNA-1
chr6_3 CDS 91870085 91870301 91870085 ID=Msa0964840-mRNA-1:cds;Parent=Msa0964840-mRNA-1
chr6_3 CDS 91870414 91870503 91870414 ID=Msa0964840-mRNA-1:cds;Parent=Msa0964840-mRNA-1
chr6_3 CDS 91870546 91870600 91870546 ID=Msa0964840-mRNA-1:cds;Parent=Msa0964840-mRNA-1
Gene Sequence

>Msa0964840

ATGTATTTTATCAAGAAATTAAGCAATAATTCTCCTACAAAGAGATCTTTAATAGTCAATTTACAATTTCGAGAACCTACACTCAACACATATTCGGATGGTGTGAAGATAAAATGTTTGCTAATGAAATATAAATTTAAAGATGAAAACATTGATTATTGGCAAGACAAAAACGATGGAGGACCTGGTTTCCTTATTGATGCTGACAAAATACGTAATAGATTACATGATTTAATTACCATATCAAAGCCAGGTTGTGAATTTAAAAAAGTGTTAGACCAAATGGCTGACGGCGTCAAGATAATTTTCATGATTGATGCTTGTTACGGAGGGGGGTTTATTGAACTAGCCCACCACAAAGTTGTTCTATTCACATCTTCCAAAAGGGATGAAGAGTCAGTCAAAGGTTCTTTGGGTTCTGTGTTTACGAATGCGTTTGTTGATTGTGTTAATGATTACCCTGGAATAACAATCAAGTTAAGAGAGTGGAATAGAGAGATGTTTGACTCCAATGAAAAAGCTAGATGCAGAAGCTTGTACTGTTTCTCTAGAGATGCTAGCGTTAATGGTCATTCTTCTTCATCTTCTGGCTCACCTTCTACTTCTTCTTGGTGA

Protein sequence

>Msa0964840

MYFIKKLSNNSPTKRSLIVNLQFREPTLNTYSDGVKIKCLLMKYKFKDENIDYWQDKNDGGPGFLIDADKIRNRLHDLITISKPGCEFKKVLDQMADGVKIIFMIDACYGGGFIELAHHKVVLFTSSKRDEESVKGSLGSVFTNAFVDCVNDYPGITIKLREWNREMFDSNEKARCRSLYCFSRDASVNGHSSSSSGSPSTSSW*