Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291330 | XP_013464264.1 | 94.828 | 116 | 6 | 0 | 1 | 116 | 1 | 116 | 3.96e-78 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291330 | sp|Q8LC53|EPF2_ARATH | 47.222 | 108 | 51 | 2 | 13 | 115 | 12 | 118 | 5.85e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0019600 | Msa0291330 | 0.836305 | 1.011672e-56 | -8.615850e-47 |
| Msa0055450 | Msa0291330 | 0.806194 | 9.258326e-50 | -8.615850e-47 |
| Msa0066320 | Msa0291330 | 0.836792 | 7.601355e-57 | -8.615850e-47 |
| Msa0124130 | Msa0291330 | 0.826216 | 3.064607e-54 | -8.615850e-47 |
| Msa0141120 | Msa0291330 | 0.800022 | 1.751014e-48 | -8.615850e-47 |
| Msa0149140 | Msa0291330 | 0.812724 | 3.670818e-51 | -8.615850e-47 |
| Msa0212080 | Msa0291330 | 0.918913 | 8.654838e-87 | -8.615850e-47 |
| Msa0252630 | Msa0291330 | 0.962856 | 2.230542e-121 | -8.615850e-47 |
| Msa0291330 | Msa0329460 | 0.816948 | 4.250726e-52 | -8.615850e-47 |
| Msa0291330 | Msa0343660 | 0.803581 | 3.255655e-49 | -8.615850e-47 |
| Msa0291330 | Msa0344570 | 0.800909 | 1.154967e-48 | -8.615850e-47 |
| Msa0291330 | Msa0351770 | 0.837238 | 5.849380e-57 | -8.615850e-47 |
| Msa0291330 | Msa0359250 | 0.854168 | 1.472871e-61 | -8.615850e-47 |
| Msa0291330 | Msa0373960 | 0.826204 | 3.084899e-54 | -8.615850e-47 |
| Msa0291330 | Msa0375680 | 0.800143 | 1.654526e-48 | -8.615850e-47 |
| Msa0291330 | Msa0392420 | 0.808736 | 2.674935e-50 | -8.615850e-47 |
| Msa0291330 | Msa0392460 | 0.802483 | 5.491658e-49 | -8.615850e-47 |
| Msa0291330 | Msa0392490 | 0.802778 | 4.773689e-49 | -8.615850e-47 |
| Msa0291330 | Msa0406000 | 0.852862 | 3.494712e-61 | -8.615850e-47 |
| Msa0291330 | Msa0415320 | 0.812350 | 4.432085e-51 | -8.615850e-47 |
| Msa0291330 | Msa0423100 | 0.831593 | 1.528895e-55 | -8.615850e-47 |
| Msa0291330 | Msa0424330 | 0.823175 | 1.595548e-53 | -8.615850e-47 |
| Msa0291330 | Msa0424340 | 0.824908 | 6.253162e-54 | -8.615850e-47 |
| Msa0291330 | Msa0424350 | 0.815111 | 1.092886e-51 | -8.615850e-47 |
| Msa0291330 | Msa0441590 | 0.802267 | 6.083829e-49 | -8.615850e-47 |
| Msa0291330 | Msa0451400 | 0.865542 | 5.477875e-65 | -8.615850e-47 |
| Msa0291330 | Msa0479540 | 0.800254 | 1.571093e-48 | -8.615850e-47 |
| Msa0291330 | Msa0487570 | 0.812954 | 3.269630e-51 | -8.615850e-47 |
| Msa0291330 | Msa0487630 | 0.802846 | 4.621562e-49 | -8.615850e-47 |
| Msa0291330 | Msa0492190 | 0.837486 | 5.053253e-57 | -8.615850e-47 |
| Msa0291330 | Msa0515950 | 0.838880 | 2.210464e-57 | -8.615850e-47 |
| Msa0291330 | Msa0534930 | 0.844751 | 6.228209e-59 | -8.615850e-47 |
| Msa0291330 | Msa0535040 | 0.802711 | 4.927837e-49 | -8.615850e-47 |
| Msa0291330 | Msa0575740 | 0.823866 | 1.099908e-53 | -8.615850e-47 |
| Msa0291330 | Msa0591790 | 0.829384 | 5.304063e-55 | -8.615850e-47 |
| Msa0291330 | Msa0591800 | 0.849309 | 3.511809e-60 | -8.615850e-47 |
| Msa0291330 | Msa0594090 | 0.813521 | 2.454688e-51 | -8.615850e-47 |
| Msa0291330 | Msa0669840 | 0.869249 | 3.566221e-66 | -8.615850e-47 |
| Msa0291330 | Msa0711320 | 0.814989 | 1.163291e-51 | -8.615850e-47 |
| Msa0291330 | Msa0725860 | 0.808999 | 2.350331e-50 | -8.615850e-47 |
| Msa0291330 | Msa0739470 | 0.828994 | 6.597276e-55 | -8.615850e-47 |
| Msa0291330 | Msa0739580 | 0.807292 | 5.428301e-50 | -8.615850e-47 |
| Msa0291330 | Msa0751890 | 0.812851 | 3.443174e-51 | -8.615850e-47 |
| Msa0291330 | Msa0764820 | 0.804349 | 2.254102e-49 | -8.615850e-47 |
| Msa0291330 | Msa0770290 | 0.817388 | 3.384953e-52 | -8.615850e-47 |
| Msa0291330 | Msa0819890 | 0.804162 | 2.465584e-49 | -8.615850e-47 |
| Msa0291330 | Msa0823400 | 0.807060 | 6.078801e-50 | -8.615850e-47 |
| Msa0291330 | Msa0831940 | 0.810586 | 1.070793e-50 | -8.615850e-47 |
| Msa0291330 | Msa0860160 | 0.806090 | 9.738298e-50 | -8.615850e-47 |
| Msa0291330 | Msa0860280 | 0.800078 | 1.705664e-48 | -8.615850e-47 |
| Msa0291330 | Msa0867350 | 0.820103 | 8.185962e-53 | -8.615850e-47 |
| Msa0291330 | Msa0898730 | 0.826262 | 2.987142e-54 | -8.615850e-47 |
| Msa0291330 | Msa0899950 | 0.816461 | 5.465954e-52 | -8.615850e-47 |
| Msa0291330 | Msa0922750 | 0.804725 | 1.882054e-49 | -8.615850e-47 |
| Msa0291330 | Msa0927120 | 0.826948 | 2.050191e-54 | -8.615850e-47 |
| Msa0291330 | Msa0929870 | 0.842096 | 3.186075e-58 | -8.615850e-47 |
| Msa0291330 | Msa0941820 | 0.801474 | 8.853277e-49 | -8.615850e-47 |
| Msa0291330 | Msa0961560 | 0.801713 | 7.908621e-49 | -8.615850e-47 |
| Msa0291330 | Msa0965310 | 0.813305 | 2.737093e-51 | -8.615850e-47 |
| Msa0291330 | Msa0996270 | 0.827909 | 1.205402e-54 | -8.615850e-47 |
| Msa0291330 | Msa0999300 | 0.800082 | 1.703031e-48 | -8.615850e-47 |
| Msa0291330 | Msa0999340 | 0.811920 | 5.499023e-51 | -8.615850e-47 |
| Msa0291330 | Msa0999350 | 0.800838 | 1.194125e-48 | -8.615850e-47 |
| Msa0291330 | Msa0999390 | 0.808609 | 2.847923e-50 | -8.615850e-47 |
| Msa0291330 | Msa0999700 | 0.809144 | 2.187450e-50 | -8.615850e-47 |
| Msa0291330 | Msa1037890 | 0.821586 | 3.732329e-53 | -8.615850e-47 |
| Msa0291330 | Msa1037900 | 0.817628 | 2.988343e-52 | -8.615850e-47 |
| Msa0291330 | Msa1084520 | 0.830251 | 3.262497e-55 | -8.615850e-47 |
| Msa0291330 | Msa1125810 | 0.816922 | 4.307255e-52 | -8.615850e-47 |
| Msa0291330 | Msa1129950 | 0.825541 | 4.432518e-54 | -8.615850e-47 |
| Msa0291330 | Msa1129960 | 0.802559 | 5.296795e-49 | -8.615850e-47 |
| Msa0291330 | Msa1130010 | 0.836561 | 8.708348e-57 | -8.615850e-47 |
| Msa0291330 | Msa1145020 | 0.822071 | 2.881797e-53 | -8.615850e-47 |
| Msa0291330 | Msa1171310 | 0.835380 | 1.736064e-56 | -8.615850e-47 |
| Msa0291330 | Msa1179360 | 0.801378 | 9.263330e-49 | -8.615850e-47 |
| Msa0291330 | Msa1201110 | 0.801658 | 8.117101e-49 | -8.615850e-47 |
| Msa0291330 | Msa1201160 | 0.838288 | 3.142728e-57 | -8.615850e-47 |
| Msa0291330 | Msa1228190 | 0.849663 | 2.798929e-60 | -8.615850e-47 |
| Msa0291330 | Msa1271260 | 0.827467 | 1.540000e-54 | -8.615850e-47 |
| Msa0291330 | Msa1294290 | 0.823926 | 1.064752e-53 | -8.615850e-47 |
| Msa0291330 | Msa1305510 | 0.820768 | 5.759752e-53 | -8.615850e-47 |
| Msa0291330 | Msa1378870 | 0.825111 | 5.601590e-54 | -8.615850e-47 |
| Msa0291330 | Msa1389050 | 0.835696 | 1.444155e-56 | -8.615850e-47 |
| Msa0291330 | Msa1406780 | 0.805399 | 1.360413e-49 | -8.615850e-47 |
| Msa0291330 | Msa1420060 | 0.808145 | 3.576937e-50 | -8.615850e-47 |
| Msa0291330 | Msa1421550 | 0.814422 | 1.553599e-51 | -8.615850e-47 |
| Msa0291330 | Msa1431850 | 0.811367 | 7.252869e-51 | -8.615850e-47 |
| Msa0291330 | Msa1435480 | 0.801948 | 7.076420e-49 | -8.615850e-47 |
| Msa0291330 | Msa1455320 | 0.801137 | 1.037865e-48 | -8.615850e-47 |
| Msa0291330 | Msa1458910 | 0.805966 | 1.034288e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291330 | MtrunA17_Chr2g0311361 | 94.118 | 68 | 4 | 0 | 49 | 116 | 31 | 98 | 1.46e-44 | 140 |
| Msa0291330 | MtrunA17_Chr2g0324101 | 59.615 | 52 | 19 | 1 | 65 | 114 | 4 | 55 | 1.53e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291330 | AT1G34245.1 | 47.222 | 108 | 51 | 2 | 13 | 115 | 12 | 118 | 5.95e-29 | 101 |
| Msa0291330 | AT1G71866.2 | 68.627 | 51 | 15 | 1 | 65 | 115 | 85 | 134 | 1.11e-21 | 84.0 |
| Msa0291330 | AT1G71866.1 | 68.627 | 51 | 15 | 1 | 65 | 115 | 121 | 170 | 4.30e-21 | 83.2 |
| Msa0291330 | AT2G20875.1 | 59.615 | 52 | 19 | 1 | 65 | 114 | 53 | 104 | 1.82e-14 | 64.3 |
Find 33 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACAATTATAGACAAAA+AGG | 0.294093 | 2_3:+39613413 | Msa0291330:CDS |
| ACATGTCACGTACCATGATT+TGG | 0.296793 | 2_3:-39613231 | None:intergenic |
| ACCTACCATGACCCTCTTGC+AGG | 0.342195 | 2_3:-39613626 | None:intergenic |
| AGGTTTCAGCAAATGTTCTA+TGG | 0.374663 | 2_3:+39613645 | Msa0291330:CDS |
| CATGATTTGGGATCACTCTA+AGG | 0.418584 | 2_3:-39613218 | None:intergenic |
| CATGTCACGTACCATGATTT+GGG | 0.459472 | 2_3:-39613230 | None:intergenic |
| AACAACTAGGAAAGATTTGT+GGG | 0.460826 | 2_3:-39613163 | None:intergenic |
| TAACAACTAGGAAAGATTTG+TGG | 0.480408 | 2_3:-39613164 | None:intergenic |
| TATGGCAGAGTCTTGCCCTA+TGG | 0.485018 | 2_3:+39613663 | Msa0291330:CDS |
| ACAATCTGGTATAGTGGAAC+CGG | 0.487458 | 2_3:-39613578 | None:intergenic |
| TTGTAGGAAGGAGCAAGAGA+AGG | 0.490101 | 2_3:+39613529 | Msa0291330:intron |
| GTTTACAGATGCATTTGTAA+AGG | 0.496002 | 2_3:+39613685 | Msa0291330:CDS |
| CCACATGCATGGGAACAATC+TGG | 0.502954 | 2_3:-39613592 | None:intergenic |
| CAAATGCATCTGTAAACCAT+AGG | 0.504305 | 2_3:-39613679 | None:intergenic |
| GTGAACATGGTCCACATGCA+TGG | 0.516739 | 2_3:-39613603 | None:intergenic |
| GTAATGGAACTCTATCCAAC+CGG | 0.520621 | 2_3:+39613559 | Msa0291330:CDS |
| ACCCTCTTGCAGGGTGAACA+TGG | 0.527410 | 2_3:-39613616 | None:intergenic |
| TCGATCAATTCGAGGGAACA+TGG | 0.534941 | 2_3:-39613717 | None:intergenic |
| AAGAGAAGGTAGCAATGTAA+TGG | 0.538637 | 2_3:+39613543 | Msa0291330:CDS |
| ATGGGAACAATCTGGTATAG+TGG | 0.545689 | 2_3:-39613584 | None:intergenic |
| TGAACATGGTCCACATGCAT+GGG | 0.559386 | 2_3:-39613602 | None:intergenic |
| AAATGCATCTGTAAACCATA+GGG | 0.566896 | 2_3:-39613678 | None:intergenic |
| CTTAGAGTGATCCCAAATCA+TGG | 0.569820 | 2_3:+39613219 | Msa0291330:CDS |
| CCGTGAAGAAAATAACAACT+AGG | 0.577940 | 2_3:-39613176 | None:intergenic |
| ATCATCGATCGATCAATTCG+AGG | 0.580959 | 2_3:-39613725 | None:intergenic |
| GACCATGTTCACCCTGCAAG+AGG | 0.594033 | 2_3:+39613614 | Msa0291330:CDS |
| TTCACCCTGCAAGAGGGTCA+TGG | 0.604879 | 2_3:+39613621 | Msa0291330:CDS |
| CCCTGCAAGAGGGTCATGGT+AGG | 0.608435 | 2_3:+39613625 | Msa0291330:CDS |
| TCATCGATCGATCAATTCGA+GGG | 0.611769 | 2_3:-39613724 | None:intergenic |
| CCTACCATGACCCTCTTGCA+GGG | 0.620825 | 2_3:-39613625 | None:intergenic |
| CCAGATTGTTCCCATGCATG+TGG | 0.632279 | 2_3:+39613592 | Msa0291330:CDS |
| TCTGGTATAGTGGAACCGGT+TGG | 0.657904 | 2_3:-39613574 | None:intergenic |
| ACCATGTTCACCCTGCAAGA+GGG | 0.681360 | 2_3:+39613615 | Msa0291330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAACAATTATAGACAAAA+AGG | + | chr2_3:39613413-39613432 | Msa0291330:CDS | 20.0% |
| ! | ACATCATTACATGATACTAT+GGG | - | chr2_3:39613308-39613327 | None:intergenic | 25.0% |
| ! | TACATCATTACATGATACTA+TGG | - | chr2_3:39613309-39613328 | None:intergenic | 25.0% |
| !!! | TTCCATATTTGTGTTTTTGT+AGG | + | chr2_3:39613513-39613532 | Msa0291330:intron | 25.0% |
| ! | TTCCTACAAAAACACAAATA+TGG | - | chr2_3:39613518-39613537 | None:intergenic | 25.0% |
| !!! | ATATTTGTGTTTTTGTAGGA+AGG | + | chr2_3:39613517-39613536 | Msa0291330:intron | 25.0% |
| AACAACTAGGAAAGATTTGT+GGG | - | chr2_3:39613166-39613185 | None:intergenic | 30.0% | |
| TAACAACTAGGAAAGATTTG+TGG | - | chr2_3:39613167-39613186 | None:intergenic | 30.0% | |
| !!! | CCTAGTTGTTATTTTCTTCA+CGG | + | chr2_3:39613176-39613195 | Msa0291330:CDS | 30.0% |
| ! | TTCTTCACGGTATTTTCTAT+TGG | + | chr2_3:39613189-39613208 | Msa0291330:CDS | 30.0% |
| AAACGAATGTGAAATACTCA+AGG | - | chr2_3:39613348-39613367 | None:intergenic | 30.0% | |
| AAGAAGTGAATGATCAATTG+TGG | + | chr2_3:39613489-39613508 | Msa0291330:intron | 30.0% | |
| AAATGCATCTGTAAACCATA+GGG | - | chr2_3:39613681-39613700 | None:intergenic | 30.0% | |
| GTTTACAGATGCATTTGTAA+AGG | + | chr2_3:39613685-39613704 | Msa0291330:CDS | 30.0% | |
| CCGTGAAGAAAATAACAACT+AGG | - | chr2_3:39613179-39613198 | None:intergenic | 35.0% | |
| ! | TCACGGTATTTTCTATTGGT+TGG | + | chr2_3:39613193-39613212 | Msa0291330:CDS | 35.0% |
| TGTAAGTGCATCAACTTATC+TGG | - | chr2_3:39613384-39613403 | None:intergenic | 35.0% | |
| AAGAGAAGGTAGCAATGTAA+TGG | + | chr2_3:39613543-39613562 | Msa0291330:CDS | 35.0% | |
| AGGTTTCAGCAAATGTTCTA+TGG | + | chr2_3:39613645-39613664 | Msa0291330:CDS | 35.0% | |
| CAAATGCATCTGTAAACCAT+AGG | - | chr2_3:39613682-39613701 | None:intergenic | 35.0% | |
| ! | CATGATTTGGGATCACTCTA+AGG | - | chr2_3:39613221-39613240 | None:intergenic | 40.0% |
| CTTAGAGTGATCCCAAATCA+TGG | + | chr2_3:39613219-39613238 | Msa0291330:CDS | 40.0% | |
| CATGTCACGTACCATGATTT+GGG | - | chr2_3:39613233-39613252 | None:intergenic | 40.0% | |
| ACATGTCACGTACCATGATT+TGG | - | chr2_3:39613234-39613253 | None:intergenic | 40.0% | |
| ! | GTAATGGAACTCTATCCAAC+CGG | + | chr2_3:39613559-39613578 | Msa0291330:CDS | 40.0% |
| ! | ACAATCTGGTATAGTGGAAC+CGG | - | chr2_3:39613581-39613600 | None:intergenic | 40.0% |
| ATGGGAACAATCTGGTATAG+TGG | - | chr2_3:39613587-39613606 | None:intergenic | 40.0% | |
| TTGTAGGAAGGAGCAAGAGA+AGG | + | chr2_3:39613529-39613548 | Msa0291330:intron | 45.0% | |
| TGAACATGGTCCACATGCAT+GGG | - | chr2_3:39613605-39613624 | None:intergenic | 45.0% | |
| ! | TCTGGTATAGTGGAACCGGT+TGG | - | chr2_3:39613577-39613596 | None:intergenic | 50.0% |
| CCACATGCATGGGAACAATC+TGG | - | chr2_3:39613595-39613614 | None:intergenic | 50.0% | |
| CCAGATTGTTCCCATGCATG+TGG | + | chr2_3:39613592-39613611 | Msa0291330:CDS | 50.0% | |
| GTGAACATGGTCCACATGCA+TGG | - | chr2_3:39613606-39613625 | None:intergenic | 50.0% | |
| ACCATGTTCACCCTGCAAGA+GGG | + | chr2_3:39613615-39613634 | Msa0291330:CDS | 50.0% | |
| TATGGCAGAGTCTTGCCCTA+TGG | + | chr2_3:39613663-39613682 | Msa0291330:CDS | 50.0% | |
| GACCATGTTCACCCTGCAAG+AGG | + | chr2_3:39613614-39613633 | Msa0291330:CDS | 55.0% | |
| ACCCTCTTGCAGGGTGAACA+TGG | - | chr2_3:39613619-39613638 | None:intergenic | 55.0% | |
| TTCACCCTGCAAGAGGGTCA+TGG | + | chr2_3:39613621-39613640 | Msa0291330:CDS | 55.0% | |
| CCTACCATGACCCTCTTGCA+GGG | - | chr2_3:39613628-39613647 | None:intergenic | 55.0% | |
| ACCTACCATGACCCTCTTGC+AGG | - | chr2_3:39613629-39613648 | None:intergenic | 55.0% | |
| CCCTGCAAGAGGGTCATGGT+AGG | + | chr2_3:39613625-39613644 | Msa0291330:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 39613141 | 39613735 | 39613141 | ID=Msa0291330;Name=Msa0291330 |
| chr2_3 | mRNA | 39613141 | 39613735 | 39613141 | ID=Msa0291330-mRNA-1;Parent=Msa0291330;Name=Msa0291330-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|3|0|116 |
| chr2_3 | exon | 39613141 | 39613240 | 39613141 | ID=Msa0291330-mRNA-1:exon:12810;Parent=Msa0291330-mRNA-1 |
| chr2_3 | exon | 39613385 | 39613434 | 39613385 | ID=Msa0291330-mRNA-1:exon:12811;Parent=Msa0291330-mRNA-1 |
| chr2_3 | exon | 39613535 | 39613735 | 39613535 | ID=Msa0291330-mRNA-1:exon:12812;Parent=Msa0291330-mRNA-1 |
| chr2_3 | CDS | 39613141 | 39613240 | 39613141 | ID=Msa0291330-mRNA-1:cds;Parent=Msa0291330-mRNA-1 |
| chr2_3 | CDS | 39613385 | 39613434 | 39613385 | ID=Msa0291330-mRNA-1:cds;Parent=Msa0291330-mRNA-1 |
| chr2_3 | CDS | 39613535 | 39613735 | 39613535 | ID=Msa0291330-mRNA-1:cds;Parent=Msa0291330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
