AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1103500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1103500 MtrunA17_Chr4g0062701 98.485 66 1 0 2 67 876 941 5.84e-40 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1103500 AT3G18524.1 45.455 66 36 0 2 67 871 936 1.33e-14 66.6

Find 32 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAATGCTTCAAGAATT+TGG 0.163300 7_3:-39374618 None:intergenic
AAGAATTTGGCGAGCCTTTA+CGG 0.238865 7_3:-39374605 None:intergenic
CAGCAATTTCTTGGCATATA+AGG 0.300692 7_3:+39374192 None:intergenic
TATTTGTTTCAGCAATTTCT+TGG 0.303880 7_3:+39374183 None:intergenic
AAATGCAACCTAATTTAAAA+AGG 0.317673 7_3:-39375047 None:intergenic
GAAATAGTGTAACCTTATTA+TGG 0.319501 7_3:+39374287 Msa1103500:exon
GTAGAATCGTGCAATGGTTC+TGG 0.354488 7_3:+39374978 Msa1103500:three_prime_UTR
GTCTAATACCATATGTCAAA+TGG 0.358396 7_3:+39374817 Msa1103500:three_prime_UTR
GACATATTACCTCCATAATA+AGG 0.360101 7_3:-39374299 None:intergenic
ACCACAACCCATTTGACATA+TGG 0.364150 7_3:-39374825 None:intergenic
AAGGAGTAGTCCAGTGGTAT+AGG 0.406046 7_3:-39374942 None:intergenic
ATACCACTGGACTACTCCTT+TGG 0.418478 7_3:+39374945 Msa1103500:three_prime_UTR
CGCAAGCTTGGCAAGAAGTA+AGG 0.428510 7_3:+39374668 Msa1103500:CDS
AAACTGATGAGATGTCTAAA+GGG 0.458547 7_3:+39374581 Msa1103500:CDS
GAAACTGATGAGATGTCTAA+AGG 0.470476 7_3:+39374580 Msa1103500:CDS
AGGATGCACAACACTGTCAT+TGG 0.479566 7_3:+39374713 Msa1103500:CDS
TCTAATACCATATGTCAAAT+GGG 0.484656 7_3:+39374818 Msa1103500:three_prime_UTR
TGTTGGTAGGTTACCCATGC+AGG 0.486815 7_3:+39374766 Msa1103500:three_prime_UTR
CCATGGACAAAACGCAAGCT+TGG 0.498752 7_3:+39374656 Msa1103500:CDS
GTTGAAAGATAATTTAGAGA+AGG 0.504906 7_3:+39374693 Msa1103500:CDS
ACCATATGTCAAATGGGTTG+TGG 0.522082 7_3:+39374824 Msa1103500:three_prime_UTR
CAAAACTCACATGTCCTGCA+TGG 0.539319 7_3:-39374780 None:intergenic
GATGTCTAAAGGGGCCGTAA+AGG 0.542377 7_3:+39374591 Msa1103500:CDS
TGGTGTCCAACCTATACCAC+TGG 0.545776 7_3:+39374932 Msa1103500:three_prime_UTR
TGCTTTGCCACTAGAAACCA+TGG 0.560545 7_3:+39374639 Msa1103500:CDS
AGTAGTCCAGTGGTATAGGT+TGG 0.570697 7_3:-39374938 None:intergenic
AAAACTCACATGTCCTGCAT+GGG 0.586431 7_3:-39374779 None:intergenic
ATAGTGTAACCTTATTATGG+AGG 0.632352 7_3:+39374290 Msa1103500:exon
TTCTACTCTAACATTACCAA+AGG 0.650856 7_3:-39374961 None:intergenic
AACTGATGAGATGTCTAAAG+GGG 0.665562 7_3:+39374582 Msa1103500:CDS
GTTAGAGTAGAATCGTGCAA+TGG 0.682443 7_3:+39374972 Msa1103500:three_prime_UTR
TTACCAAAGGAGTAGTCCAG+TGG 0.713171 7_3:-39374948 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAGTTTTCTAAATTAGATA+CGG - chr7_3:39374260-39374279 None:intergenic 20.0%
!! TTGAAATATTGAAACAACAA+GGG - chr7_3:39374349-39374368 None:intergenic 20.0%
!! ATTGAAATATTGAAACAACA+AGG - chr7_3:39374350-39374369 None:intergenic 20.0%
!! TGTCAATAATAAAAGAATAC+TGG + chr7_3:39374409-39374428 Msa1103500:intron 20.0%
!!! CAAAAATTCTAGTTGAAAAA+AGG + chr7_3:39374739-39374758 Msa1103500:exon 20.0%
!!! GTAGTTTTTTTTTTTTTCAG+TGG + chr7_3:39374852-39374871 Msa1103500:three_prime_UTR 20.0%
!!! AAAAGAGAATTTTTAGTAGT+AGG + chr7_3:39374908-39374927 Msa1103500:three_prime_UTR 20.0%
!!! GAAGTTTTCTAAATTAGATA+CGG - chr7_3:39374260-39374279 None:intergenic 20.0%
!! TTGAAATATTGAAACAACAA+GGG - chr7_3:39374349-39374368 None:intergenic 20.0%
!! ATTGAAATATTGAAACAACA+AGG - chr7_3:39374350-39374369 None:intergenic 20.0%
!! TGTCAATAATAAAAGAATAC+TGG + chr7_3:39374409-39374428 Msa1103500:intron 20.0%
!!! CAAAAATTCTAGTTGAAAAA+AGG + chr7_3:39374739-39374758 Msa1103500:exon 20.0%
!!! GTAGTTTTTTTTTTTTTCAG+TGG + chr7_3:39374852-39374871 Msa1103500:three_prime_UTR 20.0%
!!! AAAAGAGAATTTTTAGTAGT+AGG + chr7_3:39374908-39374927 Msa1103500:three_prime_UTR 20.0%
! GAAATAGTGTAACCTTATTA+TGG + chr7_3:39374287-39374306 Msa1103500:exon 25.0%
! TGAAATATTGAAACAACAAG+GGG - chr7_3:39374348-39374367 None:intergenic 25.0%
! TTTGATGAAAATTTCACCAT+TGG + chr7_3:39374494-39374513 Msa1103500:intron 25.0%
! GTTGAAAGATAATTTAGAGA+AGG + chr7_3:39374693-39374712 Msa1103500:CDS 25.0%
! TCTAATACCATATGTCAAAT+GGG + chr7_3:39374818-39374837 Msa1103500:three_prime_UTR 25.0%
! GAAATAGTGTAACCTTATTA+TGG + chr7_3:39374287-39374306 Msa1103500:exon 25.0%
! TGAAATATTGAAACAACAAG+GGG - chr7_3:39374348-39374367 None:intergenic 25.0%
! TTTGATGAAAATTTCACCAT+TGG + chr7_3:39374494-39374513 Msa1103500:intron 25.0%
! GTTGAAAGATAATTTAGAGA+AGG + chr7_3:39374693-39374712 Msa1103500:CDS 25.0%
! TCTAATACCATATGTCAAAT+GGG + chr7_3:39374818-39374837 Msa1103500:three_prime_UTR 25.0%
ATAGTGTAACCTTATTATGG+AGG + chr7_3:39374290-39374309 Msa1103500:exon 30.0%
GACATATTACCTCCATAATA+AGG - chr7_3:39374302-39374321 None:intergenic 30.0%
ATGAAAATTTCACCATTGGA+AGG + chr7_3:39374498-39374517 Msa1103500:intron 30.0%
ACTTTAATGATTCCTTCCAA+TGG - chr7_3:39374513-39374532 None:intergenic 30.0%
! GTTTTGCGCTTATAATTTTC+AGG + chr7_3:39374531-39374550 Msa1103500:intron 30.0%
AAACTGATGAGATGTCTAAA+GGG + chr7_3:39374581-39374600 Msa1103500:CDS 30.0%
CAACAAATGCTTCAAGAATT+TGG - chr7_3:39374621-39374640 None:intergenic 30.0%
AGTTGAAAAAAGGAGTTTGT+TGG + chr7_3:39374749-39374768 Msa1103500:exon 30.0%
GTCTAATACCATATGTCAAA+TGG + chr7_3:39374817-39374836 Msa1103500:three_prime_UTR 30.0%
!!! GAGAATTTTTAGTAGTAGGT+TGG + chr7_3:39374912-39374931 Msa1103500:three_prime_UTR 30.0%
TTCTACTCTAACATTACCAA+AGG - chr7_3:39374964-39374983 None:intergenic 30.0%
! TTTGAAGAGACTAAGGTTTT+TGG + chr7_3:39375013-39375032 Msa1103500:three_prime_UTR 30.0%
ATAGTGTAACCTTATTATGG+AGG + chr7_3:39374290-39374309 Msa1103500:exon 30.0%
GACATATTACCTCCATAATA+AGG - chr7_3:39374302-39374321 None:intergenic 30.0%
ATGAAAATTTCACCATTGGA+AGG + chr7_3:39374498-39374517 Msa1103500:intron 30.0%
ACTTTAATGATTCCTTCCAA+TGG - chr7_3:39374513-39374532 None:intergenic 30.0%
! GTTTTGCGCTTATAATTTTC+AGG + chr7_3:39374531-39374550 Msa1103500:intron 30.0%
AAACTGATGAGATGTCTAAA+GGG + chr7_3:39374581-39374600 Msa1103500:CDS 30.0%
CAACAAATGCTTCAAGAATT+TGG - chr7_3:39374621-39374640 None:intergenic 30.0%
AGTTGAAAAAAGGAGTTTGT+TGG + chr7_3:39374749-39374768 Msa1103500:exon 30.0%
GTCTAATACCATATGTCAAA+TGG + chr7_3:39374817-39374836 Msa1103500:three_prime_UTR 30.0%
!!! GAGAATTTTTAGTAGTAGGT+TGG + chr7_3:39374912-39374931 Msa1103500:three_prime_UTR 30.0%
TTCTACTCTAACATTACCAA+AGG - chr7_3:39374964-39374983 None:intergenic 30.0%
! TTTGAAGAGACTAAGGTTTT+TGG + chr7_3:39375013-39375032 Msa1103500:three_prime_UTR 30.0%
CACGAGTTAGAAGAAAAATC+GGG - chr7_3:39374227-39374246 None:intergenic 35.0%
CCACGAGTTAGAAGAAAAAT+CGG - chr7_3:39374228-39374247 None:intergenic 35.0%
!! TTGCGCTTATAATTTTCAGG+TGG + chr7_3:39374534-39374553 Msa1103500:intron 35.0%
!!! TGCGCTTATAATTTTCAGGT+GGG + chr7_3:39374535-39374554 Msa1103500:intron 35.0%
GAAACTGATGAGATGTCTAA+AGG + chr7_3:39374580-39374599 Msa1103500:CDS 35.0%
AACTGATGAGATGTCTAAAG+GGG + chr7_3:39374582-39374601 Msa1103500:CDS 35.0%
!! GAAAAAAGGAGTTTGTTGGT+AGG + chr7_3:39374753-39374772 Msa1103500:three_prime_UTR 35.0%
CACGAGTTAGAAGAAAAATC+GGG - chr7_3:39374227-39374246 None:intergenic 35.0%
CCACGAGTTAGAAGAAAAAT+CGG - chr7_3:39374228-39374247 None:intergenic 35.0%
!! TTGCGCTTATAATTTTCAGG+TGG + chr7_3:39374534-39374553 Msa1103500:intron 35.0%
!!! TGCGCTTATAATTTTCAGGT+GGG + chr7_3:39374535-39374554 Msa1103500:intron 35.0%
GAAACTGATGAGATGTCTAA+AGG + chr7_3:39374580-39374599 Msa1103500:CDS 35.0%
AACTGATGAGATGTCTAAAG+GGG + chr7_3:39374582-39374601 Msa1103500:CDS 35.0%
!! GAAAAAAGGAGTTTGTTGGT+AGG + chr7_3:39374753-39374772 Msa1103500:three_prime_UTR 35.0%
! CCGATTTTTCTTCTAACTCG+TGG + chr7_3:39374225-39374244 Msa1103500:five_prime_UTR 40.0%
TGAAACAACAAGGGGATTAC+AGG - chr7_3:39374340-39374359 None:intergenic 40.0%
AAGAATTTGGCGAGCCTTTA+CGG - chr7_3:39374608-39374627 None:intergenic 40.0%
!! GTTTTGTCCATGGTTTCTAG+TGG - chr7_3:39374649-39374668 None:intergenic 40.0%
AAAACTCACATGTCCTGCAT+GGG - chr7_3:39374782-39374801 None:intergenic 40.0%
!! ACCATATGTCAAATGGGTTG+TGG + chr7_3:39374824-39374843 Msa1103500:three_prime_UTR 40.0%
ACCACAACCCATTTGACATA+TGG - chr7_3:39374828-39374847 None:intergenic 40.0%
GTTAGAGTAGAATCGTGCAA+TGG + chr7_3:39374972-39374991 Msa1103500:three_prime_UTR 40.0%
!! GTGCACTTTTGAAGAGACTA+AGG + chr7_3:39375006-39375025 Msa1103500:three_prime_UTR 40.0%
! CCGATTTTTCTTCTAACTCG+TGG + chr7_3:39374225-39374244 Msa1103500:five_prime_UTR 40.0%
TGAAACAACAAGGGGATTAC+AGG - chr7_3:39374340-39374359 None:intergenic 40.0%
AAGAATTTGGCGAGCCTTTA+CGG - chr7_3:39374608-39374627 None:intergenic 40.0%
!! GTTTTGTCCATGGTTTCTAG+TGG - chr7_3:39374649-39374668 None:intergenic 40.0%
AAAACTCACATGTCCTGCAT+GGG - chr7_3:39374782-39374801 None:intergenic 40.0%
!! ACCATATGTCAAATGGGTTG+TGG + chr7_3:39374824-39374843 Msa1103500:three_prime_UTR 40.0%
ACCACAACCCATTTGACATA+TGG - chr7_3:39374828-39374847 None:intergenic 40.0%
GTTAGAGTAGAATCGTGCAA+TGG + chr7_3:39374972-39374991 Msa1103500:three_prime_UTR 40.0%
!! GTGCACTTTTGAAGAGACTA+AGG + chr7_3:39375006-39375025 Msa1103500:three_prime_UTR 40.0%
TAGAAGAAAAATCGGGCTGC+AGG - chr7_3:39374220-39374239 None:intergenic 45.0%
TGCTTTGCCACTAGAAACCA+TGG + chr7_3:39374639-39374658 Msa1103500:CDS 45.0%
AGGATGCACAACACTGTCAT+TGG + chr7_3:39374713-39374732 Msa1103500:CDS 45.0%
CAAAACTCACATGTCCTGCA+TGG - chr7_3:39374783-39374802 None:intergenic 45.0%
AGTAGTCCAGTGGTATAGGT+TGG - chr7_3:39374941-39374960 None:intergenic 45.0%
AAGGAGTAGTCCAGTGGTAT+AGG - chr7_3:39374945-39374964 None:intergenic 45.0%
ATACCACTGGACTACTCCTT+TGG + chr7_3:39374945-39374964 Msa1103500:three_prime_UTR 45.0%
TTACCAAAGGAGTAGTCCAG+TGG - chr7_3:39374951-39374970 None:intergenic 45.0%
GTAGAATCGTGCAATGGTTC+TGG + chr7_3:39374978-39374997 Msa1103500:three_prime_UTR 45.0%
TAGAAGAAAAATCGGGCTGC+AGG - chr7_3:39374220-39374239 None:intergenic 45.0%
TGCTTTGCCACTAGAAACCA+TGG + chr7_3:39374639-39374658 Msa1103500:CDS 45.0%
AGGATGCACAACACTGTCAT+TGG + chr7_3:39374713-39374732 Msa1103500:CDS 45.0%
CAAAACTCACATGTCCTGCA+TGG - chr7_3:39374783-39374802 None:intergenic 45.0%
AGTAGTCCAGTGGTATAGGT+TGG - chr7_3:39374941-39374960 None:intergenic 45.0%
AAGGAGTAGTCCAGTGGTAT+AGG - chr7_3:39374945-39374964 None:intergenic 45.0%
ATACCACTGGACTACTCCTT+TGG + chr7_3:39374945-39374964 Msa1103500:three_prime_UTR 45.0%
TTACCAAAGGAGTAGTCCAG+TGG - chr7_3:39374951-39374970 None:intergenic 45.0%
GTAGAATCGTGCAATGGTTC+TGG + chr7_3:39374978-39374997 Msa1103500:three_prime_UTR 45.0%
GATGTCTAAAGGGGCCGTAA+AGG + chr7_3:39374591-39374610 Msa1103500:CDS 50.0%
! CCAAGCTTGCGTTTTGTCCA+TGG - chr7_3:39374659-39374678 None:intergenic 50.0%
CCATGGACAAAACGCAAGCT+TGG + chr7_3:39374656-39374675 Msa1103500:CDS 50.0%
CGCAAGCTTGGCAAGAAGTA+AGG + chr7_3:39374668-39374687 Msa1103500:CDS 50.0%
! TGTTGGTAGGTTACCCATGC+AGG + chr7_3:39374766-39374785 Msa1103500:three_prime_UTR 50.0%
TGGTGTCCAACCTATACCAC+TGG + chr7_3:39374932-39374951 Msa1103500:three_prime_UTR 50.0%
GATGTCTAAAGGGGCCGTAA+AGG + chr7_3:39374591-39374610 Msa1103500:CDS 50.0%
! CCAAGCTTGCGTTTTGTCCA+TGG - chr7_3:39374659-39374678 None:intergenic 50.0%
CCATGGACAAAACGCAAGCT+TGG + chr7_3:39374656-39374675 Msa1103500:CDS 50.0%
CGCAAGCTTGGCAAGAAGTA+AGG + chr7_3:39374668-39374687 Msa1103500:CDS 50.0%
! TGTTGGTAGGTTACCCATGC+AGG + chr7_3:39374766-39374785 Msa1103500:three_prime_UTR 50.0%
TGGTGTCCAACCTATACCAC+TGG + chr7_3:39374932-39374951 Msa1103500:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 39374193 39375057 39374193 ID=Msa1103500;Name=Msa1103500
chr7_3 mRNA 39374193 39375057 39374193 ID=Msa1103500-mRNA-1;Parent=Msa1103500;Name=Msa1103500-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113|1|1|1|1|1|2|307|67
chr7_3 exon 39374193 39374311 39374193 ID=Msa1103500-mRNA-1:exon:7969;Parent=Msa1103500-mRNA-1
chr7_3 exon 39374553 39375057 39374553 ID=Msa1103500-mRNA-1:exon:7970;Parent=Msa1103500-mRNA-1
chr7_3 five_prime_UTR 39374193 39374305 39374193 ID=Msa1103500-mRNA-1:five_prime_utr;Parent=Msa1103500-mRNA-1
chr7_3 CDS 39374306 39374311 39374306 ID=Msa1103500-mRNA-1:cds;Parent=Msa1103500-mRNA-1
chr7_3 CDS 39374553 39374750 39374553 ID=Msa1103500-mRNA-1:cds;Parent=Msa1103500-mRNA-1
chr7_3 three_prime_UTR 39374751 39375057 39374751 ID=Msa1103500-mRNA-1:three_prime_utr;Parent=Msa1103500-mRNA-1
Gene Sequence

>Msa1103500

ATGGAGGTGGGTTCTAAACGTAAGAGAGTGTTTGAAACTGATGAGATGTCTAAAGGGGCCGTAAAGGCTCGCCAAATTCTTGAAGCATTTGTTGCTTTGCCACTAGAAACCATGGACAAAACGCAAGCTTGGCAAGAAGTAAGGAAGTTGAAAGATAATTTAGAGAAGGATGCACAACACTGTCATTGGTTGCAAAAATTCTAG

Protein sequence

>Msa1103500

MEVGSKRKRVFETDEMSKGAVKARQILEAFVALPLETMDKTQAWQEVRKLKDNLEKDAQHCHWLQKF*