AlfalfaGEDB | Msa1261000

M. sativa cultivar zhongmu-4 / Msa1261000

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261000	XP_013458057.1	98.350	303	5	0	1	303	1	303	0.0	619

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261000	sp\|Q9FNN6\|SRM1_ARATH	51.027	292	95	9	3	283	7	261	4.29e-80	248

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261000	A0A072USM0	98.350	303	5	0	1	303	1	303	0.0	619

TFs/TRs:

Gene ID	Type	Classification
Msa1261000	TF	MYB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017120	Msa1261000	0.824099	9.695389e-54	-8.615850e-47
Msa0035090	Msa1261000	0.802182	6.333911e-49	-8.615850e-47
Msa0050730	Msa1261000	0.806338	8.633338e-50	-8.615850e-47
Msa0060600	Msa1261000	0.801643	8.171743e-49	-8.615850e-47
Msa0065390	Msa1261000	0.816398	5.643754e-52	-8.615850e-47
Msa0075850	Msa1261000	0.808395	3.163548e-50	-8.615850e-47
Msa0094420	Msa1261000	0.814633	1.395125e-51	-8.615850e-47
Msa0110710	Msa1261000	0.820059	8.375734e-53	-8.615850e-47
Msa0606060	Msa1261000	0.820762	5.777932e-53	-8.615850e-47
Msa0622390	Msa1261000	0.835462	1.654316e-56	-8.615850e-47
Msa0633880	Msa1261000	0.801710	7.917883e-49	-8.615850e-47
Msa0686610	Msa1261000	0.819206	1.311713e-52	-8.615850e-47
Msa0704570	Msa1261000	0.801994	6.923628e-49	-8.615850e-47
Msa0714020	Msa1261000	0.807587	4.700010e-50	-8.615850e-47
Msa0714160	Msa1261000	0.802001	6.902265e-49	-8.615850e-47
Msa0740420	Msa1261000	0.826017	3.417352e-54	-8.615850e-47
Msa0748130	Msa1261000	0.817885	2.614174e-52	-8.615850e-47
Msa0757110	Msa1261000	0.816017	6.869263e-52	-8.615850e-47
Msa0772150	Msa1261000	0.802649	5.076120e-49	-8.615850e-47
Msa0820670	Msa1261000	0.809711	1.653313e-50	-8.615850e-47
Msa0836190	Msa1261000	0.824765	6.758667e-54	-8.615850e-47
Msa0130120	Msa1261000	0.809636	1.715349e-50	-8.615850e-47
Msa0141670	Msa1261000	0.806707	7.216344e-50	-8.615850e-47
Msa0157020	Msa1261000	0.830373	3.046954e-55	-8.615850e-47
Msa0162030	Msa1261000	0.810363	1.196271e-50	-8.615850e-47
Msa0168390	Msa1261000	0.801670	8.070331e-49	-8.615850e-47
Msa0175900	Msa1261000	0.817026	4.082449e-52	-8.615850e-47
Msa0207180	Msa1261000	0.831977	1.229046e-55	-8.615850e-47
Msa0208930	Msa1261000	0.809087	2.250689e-50	-8.615850e-47
Msa0227500	Msa1261000	0.803877	2.825999e-49	-8.615850e-47
Msa0230400	Msa1261000	0.822877	1.872022e-53	-8.615850e-47
Msa1248400	Msa1261000	0.823374	1.433963e-53	-8.615850e-47
Msa1250670	Msa1261000	0.804758	1.852023e-49	-8.615850e-47
Msa1261000	Msa1289340	0.804043	2.610816e-49	-8.615850e-47
Msa1261000	Msa1300500	0.942793	3.823340e-102	-8.615850e-47
Msa1261000	Msa1302150	0.819539	1.101558e-52	-8.615850e-47
Msa1261000	Msa1325980	0.804143	2.488746e-49	-8.615850e-47
Msa1261000	Msa1327820	0.839872	1.221578e-57	-8.615850e-47
Msa1261000	Msa1352820	0.807229	5.596346e-50	-8.615850e-47
Msa1261000	Msa1367390	-0.803553	3.299946e-49	-8.615850e-47
Msa1261000	Msa1367940	0.813719	2.220497e-51	-8.615850e-47
Msa1261000	Msa1379070	0.811781	5.897643e-51	-8.615850e-47
Msa1261000	Msa1379090	0.806478	8.068645e-50	-8.615850e-47
Msa1261000	Msa1388230	0.806658	7.392340e-50	-8.615850e-47
Msa1261000	Msa1397540	0.841605	4.295014e-58	-8.615850e-47
Msa1261000	Msa1439800	0.801195	1.009445e-48	-8.615850e-47
Msa1261000	Msa1456650	0.814547	1.457356e-51	-8.615850e-47
Msa0371610	Msa1261000	0.814599	1.419398e-51	-8.615850e-47
Msa0380920	Msa1261000	0.809226	2.101438e-50	-8.615850e-47
Msa0392410	Msa1261000	0.802058	6.717641e-49	-8.615850e-47
Msa0392450	Msa1261000	0.806907	6.547445e-50	-8.615850e-47
Msa0392480	Msa1261000	0.806124	9.581180e-50	-8.615850e-47
Msa0420070	Msa1261000	0.815054	1.125373e-51	-8.615850e-47
Msa0423110	Msa1261000	0.801510	8.703223e-49	-8.615850e-47
Msa0427050	Msa1261000	0.807551	4.781993e-50	-8.615850e-47
Msa0466100	Msa1261000	0.817410	3.346335e-52	-8.615850e-47
Msa0996090	Msa1261000	0.803287	3.746361e-49	-8.615850e-47
Msa1020710	Msa1261000	0.808589	2.874886e-50	-8.615850e-47
Msa1040770	Msa1261000	0.802702	4.949880e-49	-8.615850e-47
Msa1044040	Msa1261000	0.803683	3.100706e-49	-8.615850e-47
Msa1092820	Msa1261000	0.806522	7.895970e-50	-8.615850e-47
Msa0253370	Msa1261000	0.800651	1.304360e-48	-8.615850e-47
Msa0253410	Msa1261000	0.805360	1.385931e-49	-8.615850e-47
Msa0274780	Msa1261000	0.831663	1.469674e-55	-8.615850e-47
Msa0284780	Msa1261000	0.822190	2.704578e-53	-8.615850e-47
Msa0315480	Msa1261000	0.842376	2.685699e-58	-8.615850e-47
Msa0344580	Msa1261000	0.812735	3.650281e-51	-8.615850e-47
Msa0349000	Msa1261000	0.803283	3.753763e-49	-8.615850e-47
Msa1149760	Msa1261000	0.816294	5.954336e-52	-8.615850e-47
Msa1181380	Msa1261000	0.800398	1.468418e-48	-8.615850e-47
Msa1195860	Msa1261000	0.818287	2.120577e-52	-8.615850e-47
Msa1200920	Msa1261000	0.822605	2.166674e-53	-8.615850e-47
Msa1214400	Msa1261000	0.949200	2.058358e-107	-8.615850e-47
Msa0487560	Msa1261000	0.822755	1.999224e-53	-8.615850e-47
Msa0487620	Msa1261000	0.828810	7.307415e-55	-8.615850e-47
Msa0511890	Msa1261000	0.825259	5.168397e-54	-8.615850e-47
Msa0524200	Msa1261000	-0.805589	1.241013e-49	-8.615850e-47
Msa0530210	Msa1261000	0.818134	2.296904e-52	-8.615850e-47
Msa0594180	Msa1261000	0.831309	1.796008e-55	-8.615850e-47
Msa0922540	Msa1261000	0.811418	7.069763e-51	-8.615850e-47
Msa0955310	Msa1261000	0.852390	4.764092e-61	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261000	MtrunA17_Chr4g0062741	98.350	303	5	0	1	303	1	303	0.0	619
Msa1261000	MtrunA17_Chr1g0162081	52.131	305	103	9	8	296	12	289	7.09e-90	270
Msa1261000	MtrunA17_Chr3g0104071	48.804	209	71	5	8	211	43	220	4.54e-56	184
Msa1261000	MtrunA17_Chr5g0429521	48.039	204	69	4	8	206	29	200	1.40e-55	182
Msa1261000	MtrunA17_Chr7g0241761	37.171	304	141	9	3	288	30	301	9.59e-55	180
Msa1261000	MtrunA17_Chr1g0210471	45.500	200	79	3	8	207	24	193	3.12e-53	174
Msa1261000	MtrunA17_Chr5g0436501	40.952	210	84	5	8	212	27	201	5.12e-47	159
Msa1261000	MtrunA17_Chr8g0365011	34.862	327	133	12	1	296	1	278	6.50e-46	156
Msa1261000	MtrunA17_Chr4g0055931	67.059	85	28	0	127	211	84	168	7.14e-35	129
Msa1261000	MtrunA17_Chr6g0488471	72.222	72	20	0	132	203	91	162	6.96e-34	125
Msa1261000	MtrunA17_Chr8g0389241	73.239	71	18	1	132	202	99	168	2.55e-33	123
Msa1261000	MtrunA17_Chr2g0331031	60.638	94	30	1	109	202	87	173	1.65e-32	123
Msa1261000	MtrunA17_Chr8g0364731	50.685	146	49	7	147	278	10	146	6.66e-30	112
Msa1261000	MtrunA17_Chr5g0415191	59.184	98	30	1	107	204	81	168	1.40e-27	108
Msa1261000	MtrunA17_Chr3g0092621	51.456	103	39	1	113	204	48	150	1.30e-26	102
Msa1261000	MtrunA17_Chr4g0059701	68.354	79	25	0	126	204	72	150	1.38e-25	103
Msa1261000	MtrunA17_Chr8g0364741	38.776	147	50	5	4	147	6	115	1.25e-24	96.3
Msa1261000	MtrunA17_Chr5g0409841	50.617	81	38	1	134	212	88	168	6.63e-23	94.7
Msa1261000	MtrunA17_Chr5g0440731	54.430	79	32	2	134	209	101	178	8.63e-23	95.9
Msa1261000	MtrunA17_Chr3g0135671	56.410	78	31	2	128	202	81	158	1.22e-22	94.0
Msa1261000	MtrunA17_Chr5g0440741	56.410	78	29	3	135	209	98	173	5.61e-22	93.6
Msa1261000	MtrunA17_Chr6g0452791	57.746	71	20	1	138	208	80	140	7.13e-20	85.1
Msa1261000	MtrunA17_Chr5g0409831	46.067	89	45	1	127	212	66	154	3.51e-18	81.6
Msa1261000	MtrunA17_Chr5g0432921	32.338	201	93	10	8	205	21	181	3.73e-18	81.6
Msa1261000	MtrunA17_Chr5g0432941	32.836	201	91	10	8	205	22	181	5.12e-17	78.6
Msa1261000	MtrunA17_Chr1g0155181	46.667	75	36	2	1	75	1	71	1.41e-16	73.6
Msa1261000	MtrunA17_Chr3g0141601	46.667	75	37	2	3	77	2	73	3.33e-16	72.8
Msa1261000	MtrunA17_Chr6g0449111	45.946	74	37	1	2	75	4	74	2.54e-14	67.0
Msa1261000	MtrunA17_Chr1g0192061	48.611	72	33	2	140	208	63	133	4.55e-14	69.3
Msa1261000	MtrunA17_Chr3g0144631	40.000	70	39	1	4	73	12	78	8.17e-13	63.2
Msa1261000	MtrunA17_Chr8g0370121	47.170	53	28	0	135	187	97	149	6.69e-12	64.3
Msa1261000	MtrunA17_Chr3g0113121	45.946	74	33	2	140	206	97	170	8.01e-11	60.8
Msa1261000	MtrunA17_Chr3g0081631	43.421	76	38	3	1	75	4	75	8.62e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261000	AT1G49010.1	54.328	335	99	11	1	302	1	314	5.28e-95	283
Msa1261000	AT5G08520.1	51.027	292	95	9	3	283	7	261	4.37e-81	248
Msa1261000	AT2G38090.1	45.050	202	93	2	5	206	26	209	9.02e-57	185
Msa1261000	AT5G58900.1	46.512	215	84	6	2	212	28	215	2.22e-54	179
Msa1261000	AT5G23650.1	44.643	224	96	5	3	224	8	205	5.44e-51	171
Msa1261000	AT5G01200.1	45.098	204	92	3	6	206	28	214	6.93e-51	169
Msa1261000	AT5G04760.1	41.553	219	91	4	8	226	6	187	1.23e-47	159
Msa1261000	AT3G11280.2	44.724	199	74	3	8	206	33	195	1.79e-47	160
Msa1261000	AT3G11280.1	44.724	199	74	3	8	206	33	195	1.79e-47	160
Msa1261000	AT5G05790.2	42.233	206	89	3	1	206	24	199	6.36e-47	159
Msa1261000	AT5G05790.1	42.233	206	89	3	1	206	24	199	6.36e-47	159
Msa1261000	AT5G47390.1	57.798	109	43	1	112	217	67	175	9.81e-35	129
Msa1261000	AT3G10580.2	37.295	244	89	11	1	244	1	180	1.14e-33	124
Msa1261000	AT3G10580.1	37.295	244	89	11	1	244	1	180	1.83e-33	124
Msa1261000	AT5G61620.1	38.265	196	89	7	112	298	84	256	8.56e-31	118
Msa1261000	AT5G56840.1	56.818	88	37	1	121	207	74	161	4.37e-29	111
Msa1261000	AT4G09450.1	35.683	227	92	7	8	229	7	184	3.05e-28	108
Msa1261000	AT1G70000.1	61.458	96	27	1	108	203	78	163	2.57e-27	107
Msa1261000	AT1G70000.2	61.458	96	27	1	108	203	78	163	2.57e-27	107
Msa1261000	AT1G74840.2	53.043	115	51	1	116	227	74	188	4.12e-26	103
Msa1261000	AT1G74840.1	53.043	115	51	1	116	227	74	188	4.90e-26	104
Msa1261000	AT3G16350.1	83.019	53	9	0	133	185	132	184	9.44e-26	105
Msa1261000	AT1G19000.2	68.831	77	24	0	128	204	92	168	2.08e-23	97.4
Msa1261000	AT1G19000.1	68.831	77	24	0	128	204	92	168	2.08e-23	97.4
Msa1261000	AT3G10590.1	47.917	96	48	2	123	217	96	190	5.24e-18	81.3
Msa1261000	AT4G39250.1	40.909	66	36	1	8	73	14	76	1.49e-12	63.2
Msa1261000	AT1G75250.2	39.437	71	40	1	3	73	7	74	1.91e-12	62.8
Msa1261000	AT1G75250.1	39.437	71	40	1	3	73	7	74	5.65e-12	62.4

Find 106 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGGATTTCTTTGGAATTA+TGG	0.155681	8_2:-63248259	None:intergenic
AAAAGATCAAGTTGTAATTT+TGG	0.168041	8_2:+63248578	Msa1261000:CDS
TCTAATTCATTAGCATAATT+TGG	0.179151	8_2:-63248503	None:intergenic
AGTTTAGGAGAGTTGGATTT+TGG	0.179396	8_2:+63249539	Msa1261000:three_prime_UTR
TTACTTGGTTTAGACAAATT+TGG	0.202969	8_2:+63249034	Msa1261000:CDS
CATCATCCTCCTGGTGGTTT+AGG	0.251182	8_2:+63249319	Msa1261000:CDS
GGCCCTTGGTTGGATTTCTT+TGG	0.265789	8_2:-63248267	None:intergenic
CCTTATTAGGTGGTGGGTCT+TGG	0.278133	8_2:+63249843	Msa1261000:three_prime_UTR
CCTTCAAATACAACGGGTTT+AGG	0.282083	8_2:+63249268	Msa1261000:CDS
TTACCATGTTGACCTGTTAT+TGG	0.282124	8_2:-63249238	None:intergenic
CCAAGACCCACCACCTAATA+AGG	0.287986	8_2:-63249843	None:intergenic
TTCAAACAAAGATTCTTCTA+AGG	0.298166	8_2:+63248538	Msa1261000:CDS
ACAAATTTGGAAAAGGAGAT+TGG	0.304845	8_2:+63249047	Msa1261000:CDS
ATTTAGTAGATTTCCTTATT+AGG	0.311305	8_2:+63249830	Msa1261000:three_prime_UTR
ATCTGTCCCCAACAAAAGTA+TGG	0.337048	8_2:+63248409	Msa1261000:CDS
TTTGGAGGCTCGTCAGGGTC+GGG	0.344715	8_2:+63249557	Msa1261000:three_prime_UTR
AAAATAAAGTTTAGGAGAGT+TGG	0.358256	8_2:+63249532	Msa1261000:three_prime_UTR
CAAGGAACTTTGTGATATCT+AGG	0.372284	8_2:+63249080	Msa1261000:CDS
TGTTGCTCCTCCTGCTCATA+TGG	0.385813	8_2:+63249372	Msa1261000:CDS
AATCATCAAGCACCAATAAC+AGG	0.394644	8_2:+63249226	Msa1261000:CDS
CCTAAACCCGTTGTATTTGA+AGG	0.403324	8_2:-63249268	None:intergenic
TAGATTTCCTTATTAGGTGG+TGG	0.405451	8_2:+63249836	Msa1261000:three_prime_UTR
ATCCACCTTTACCACTACTA+TGG	0.406819	8_2:-63248637	None:intergenic
GGATGATGGGGAAGCATAAC+AGG	0.408632	8_2:-63249412	None:intergenic
TTCTTCTTCATAGCTCCAAA+TGG	0.430907	8_2:-63248309	None:intergenic
CTTGATGACATGTTGGCCCT+TGG	0.437686	8_2:-63248281	None:intergenic
CCTGGTGGTTTAGGAATGTA+TGG	0.438589	8_2:+63249328	Msa1261000:CDS
CTTAACTTTCCACATAAAAG+AGG	0.441939	8_2:-63249704	None:intergenic
TGTCATCAAGTGGAGCCATT+TGG	0.441940	8_2:+63248294	Msa1261000:CDS
GCTCATATGGCATCTGCAGT+TGG	0.444181	8_2:+63249385	Msa1261000:CDS
AGTTGTCATTGGTGCATTGT+TGG	0.444498	8_2:-63249484	None:intergenic
ACATGACATGATCATAATCT+TGG	0.445405	8_2:-63249929	None:intergenic
ACAATCCCTTCAAATACAAC+GGG	0.448250	8_2:+63249262	Msa1261000:CDS
ATGCACAAAAGTATTTCATA+AGG	0.449793	8_2:+63249125	Msa1261000:CDS
AGAAGATTCAAAAGAACAAT+GGG	0.451020	8_2:+63248373	Msa1261000:CDS
CTATGCTCTTCTTCTGTCCA+TGG	0.458260	8_2:-63248707	None:intergenic
CTCATATGGCATCTGCAGTT+GGG	0.466530	8_2:+63249386	Msa1261000:CDS
GCATGACTTGCTACTTGTGT+TGG	0.467928	8_2:-63249106	None:intergenic
CCAACATTGGTTGGTTCAGT+TGG	0.475875	8_2:-63249625	None:intergenic
CAAAGTTTAGAGAATGAGAA+TGG	0.483834	8_2:-63248234	None:intergenic
GGTTTAGACAAATTTGGAAA+AGG	0.486380	8_2:+63249040	Msa1261000:CDS
AGATTTCCTTATTAGGTGGT+GGG	0.487579	8_2:+63249837	Msa1261000:three_prime_UTR
TTGGAGGCTCGTCAGGGTCG+GGG	0.488821	8_2:+63249558	Msa1261000:three_prime_UTR
TCAGAACAAGAAAGGAGAAA+AGG	0.492641	8_2:+63248680	Msa1261000:CDS
TGTTAACACTAGTAATATCA+TGG	0.493710	8_2:-63249186	None:intergenic
ATGACATGTTGGCCCTTGGT+TGG	0.495450	8_2:-63248277	None:intergenic
TGAGCAGGAGGAGCAACAAC+AGG	0.495632	8_2:-63249367	None:intergenic
TATGATTATATTAAATTCGA+AGG	0.499746	8_2:+63249882	Msa1261000:three_prime_UTR
ATGGTGAAATTAATTGAAGA+TGG	0.500024	8_2:-63248215	None:intergenic
GTGGATGATGATGAGGATGA+TGG	0.500151	8_2:-63249426	None:intergenic
TACATGACATGGTAGTATAG+AGG	0.500647	8_2:-63248145	None:intergenic
CATCATCATCAGAACAAGAA+AGG	0.501232	8_2:+63248672	Msa1261000:CDS
GTTCAGCCTCATCATCCTCC+TGG	0.503699	8_2:+63249310	Msa1261000:CDS
TGGATGATGATGAGGATGAT+GGG	0.504027	8_2:-63249425	None:intergenic
TGTACCTCTTGCTTACCCTA+TGG	0.504295	8_2:+63249456	Msa1261000:CDS
CTAAACCCGTTGTATTTGAA+GGG	0.506036	8_2:-63249267	None:intergenic
TAAGCAAGAGGTACAACATA+AGG	0.508613	8_2:-63249448	None:intergenic
TGCATTGTTGGAGGTGCCAT+AGG	0.508759	8_2:-63249472	None:intergenic
TGTCATTGGTGCATTGTTGG+AGG	0.512443	8_2:-63249481	None:intergenic
AGAATGCTATAGCCATGCAT+TGG	0.517213	8_2:+63248342	Msa1261000:CDS
CACCTTTACCACTACTATGG+TGG	0.519881	8_2:-63248634	None:intergenic
TAACAACAAATAAAAGAAAG+AGG	0.520903	8_2:-63249655	None:intergenic
CCAACTGAACCAACCAATGT+TGG	0.532207	8_2:+63249625	Msa1261000:three_prime_UTR
GCAACAACAGGATGTCCTAC+TGG	0.541033	8_2:-63249355	None:intergenic
GGAGTTGAGCCAACATTGGT+TGG	0.541325	8_2:-63249634	None:intergenic
ACCCACCATAGTAGTGGTAA+AGG	0.542637	8_2:+63248632	Msa1261000:CDS
AAGAAGATTCAAAAGAACAA+TGG	0.544714	8_2:+63248372	Msa1261000:CDS
CACAATCCCTTCAAATACAA+CGG	0.544826	8_2:+63249261	Msa1261000:CDS
GAGATTGGAGAAGTATATCA+AGG	0.547704	8_2:+63249062	Msa1261000:CDS
AAAGGAGAAAAGGAATTCCA+TGG	0.553013	8_2:+63248690	Msa1261000:CDS
ACTGCAGATGCCATATGAGC+AGG	0.554621	8_2:-63249382	None:intergenic
TAACATCATCTGCCAACACT+TGG	0.564260	8_2:-63248451	None:intergenic
CAATGAATAGAGATAGAAGA+AGG	0.573297	8_2:+63249155	Msa1261000:CDS
AAGAGGAGTTGAGCCAACAT+TGG	0.577251	8_2:-63249638	None:intergenic
TAGTAGATTTCCTTATTAGG+TGG	0.580399	8_2:+63249833	Msa1261000:three_prime_UTR
TGGTAGTATAGAGGGTGCGC+AGG	0.586308	8_2:-63248136	None:intergenic
ACCTTTACCACTACTATGGT+GGG	0.587562	8_2:-63248633	None:intergenic
AACTATTTATTAGCATCTCT+AGG	0.588023	8_2:-63249954	None:intergenic
CAGCCTCATCATCCTCCTGG+TGG	0.590947	8_2:+63249313	Msa1261000:CDS
TGGTGAAATTAATTGAAGAT+GGG	0.591913	8_2:-63248214	None:intergenic
CACCATAGTAGTGGTAAAGG+TGG	0.591929	8_2:+63248635	Msa1261000:CDS
ACATGACATGGTAGTATAGA+GGG	0.592564	8_2:-63248144	None:intergenic
AGATGATGTTAGTGCAATTG+AGG	0.593055	8_2:+63248463	Msa1261000:CDS
GAAACAACATTACCAAGTGT+TGG	0.597849	8_2:+63248439	Msa1261000:CDS
GGATGATGATGAGGATGATG+GGG	0.600297	8_2:-63249424	None:intergenic
GCAAGAGGTACAACATAAGG+TGG	0.601447	8_2:-63249445	None:intergenic
GATGTTAGTGCAATTGAGGA+AGG	0.604039	8_2:+63248467	Msa1261000:CDS
GGCTCCACTTGATGACATGT+TGG	0.607903	8_2:-63248288	None:intergenic
TTCCAAAGAAATCCAACCAA+GGG	0.612163	8_2:+63248265	Msa1261000:five_prime_UTR
GACTCAGAGTGTAGTGACAG+AGG	0.619139	8_2:+63249591	Msa1261000:three_prime_UTR
AGGGCCAACATGTCATCAAG+TGG	0.623051	8_2:+63248284	Msa1261000:exon
CCATACATTCCTAAACCACC+AGG	0.623664	8_2:-63249328	None:intergenic
GACTCTACCCACCATAGTAG+TGG	0.624946	8_2:+63248626	Msa1261000:CDS
AAACCACCAGGAGGATGATG+AGG	0.627717	8_2:-63249316	None:intergenic
GTGGTAAAGGTGGATTGTCA+AGG	0.632578	8_2:+63248645	Msa1261000:CDS
GCATTGTTGGAGGTGCCATA+GGG	0.632899	8_2:-63249471	None:intergenic
CATGGACAGAAGAAGAGCAT+AGG	0.635255	8_2:+63248708	Msa1261000:CDS
GATATTACTAGTGTTAACAA+TGG	0.637555	8_2:+63249190	Msa1261000:CDS
GGAATGTATGGAGCACCAGT+AGG	0.638071	8_2:+63249340	Msa1261000:CDS
GCACCAATAACAGGTCAACA+TGG	0.640651	8_2:+63249235	Msa1261000:CDS
GCAGATGCCATATGAGCAGG+AGG	0.646062	8_2:-63249379	None:intergenic
ACATAAGGTGGATGATGATG+AGG	0.659765	8_2:-63249433	None:intergenic
GAATTTGTCTCTACATGACA+TGG	0.661482	8_2:-63248156	None:intergenic
ATTCCAAAGAAATCCAACCA+AGG	0.673272	8_2:+63248264	Msa1261000:five_prime_UTR
GGTGCCATAGGGTAAGCAAG+AGG	0.704654	8_2:-63249460	None:intergenic
TACATTCCTAAACCACCAGG+AGG	0.734599	8_2:-63249325	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTTTCTTTTTTTTTTTTTC+AGG	+	chr8_2:63249005-63249024	Msa1261000:intron	15.0%
!!	TATGATTATATTAAATTCGA+AGG	+	chr8_2:63249882-63249901	Msa1261000:three_prime_UTR	15.0%
!!!	TGTTTCTTTTTTTTTTTTTC+AGG	+	chr8_2:63249005-63249024	Msa1261000:intron	15.0%
!!	TATGATTATATTAAATTCGA+AGG	+	chr8_2:63249882-63249901	Msa1261000:three_prime_UTR	15.0%
!!	TCTAATTCATTAGCATAATT+TGG	-	chr8_2:63248506-63248525	None:intergenic	20.0%
!!!	AAAAGATCAAGTTGTAATTT+TGG	+	chr8_2:63248578-63248597	Msa1261000:CDS	20.0%
!!	TTCAAGGTAATTAATTAACA+TGG	+	chr8_2:63248956-63248975	Msa1261000:intron	20.0%
!!!	TTTTTCAGGTTATTTTTACT+TGG	+	chr8_2:63249019-63249038	Msa1261000:intron	20.0%
!!	AAAAAAAAAAGAGTTGTCAT+TGG	-	chr8_2:63249498-63249517	None:intergenic	20.0%
!!!	TTTTGTCGAAAATAAAGTTT+AGG	+	chr8_2:63249524-63249543	Msa1261000:three_prime_UTR	20.0%
!!	TAACAACAAATAAAAGAAAG+AGG	-	chr8_2:63249658-63249677	None:intergenic	20.0%
!!!	ATTTGTTGTTAGTTTTTATG+AGG	+	chr8_2:63249667-63249686	Msa1261000:three_prime_UTR	20.0%
!!	ATTTACTCAAAAAGAATTTG+AGG	+	chr8_2:63249761-63249780	Msa1261000:three_prime_UTR	20.0%
!!	ATTTAGTAGATTTCCTTATT+AGG	+	chr8_2:63249830-63249849	Msa1261000:three_prime_UTR	20.0%
!!	TCTAATTCATTAGCATAATT+TGG	-	chr8_2:63248506-63248525	None:intergenic	20.0%
!!!	AAAAGATCAAGTTGTAATTT+TGG	+	chr8_2:63248578-63248597	Msa1261000:CDS	20.0%
!!	TTCAAGGTAATTAATTAACA+TGG	+	chr8_2:63248956-63248975	Msa1261000:intron	20.0%
!!!	TTTTTCAGGTTATTTTTACT+TGG	+	chr8_2:63249019-63249038	Msa1261000:intron	20.0%
!!	AAAAAAAAAAGAGTTGTCAT+TGG	-	chr8_2:63249498-63249517	None:intergenic	20.0%
!!!	TTTTGTCGAAAATAAAGTTT+AGG	+	chr8_2:63249524-63249543	Msa1261000:three_prime_UTR	20.0%
!!	TAACAACAAATAAAAGAAAG+AGG	-	chr8_2:63249658-63249677	None:intergenic	20.0%
!!!	ATTTGTTGTTAGTTTTTATG+AGG	+	chr8_2:63249667-63249686	Msa1261000:three_prime_UTR	20.0%
!!	ATTTACTCAAAAAGAATTTG+AGG	+	chr8_2:63249761-63249780	Msa1261000:three_prime_UTR	20.0%
!!	ATTTAGTAGATTTCCTTATT+AGG	+	chr8_2:63249830-63249849	Msa1261000:three_prime_UTR	20.0%
!	TGGTGAAATTAATTGAAGAT+GGG	-	chr8_2:63248217-63248236	None:intergenic	25.0%
!	ATGGTGAAATTAATTGAAGA+TGG	-	chr8_2:63248218-63248237	None:intergenic	25.0%
!	AAGAAGATTCAAAAGAACAA+TGG	+	chr8_2:63248372-63248391	Msa1261000:CDS	25.0%
!	AGAAGATTCAAAAGAACAAT+GGG	+	chr8_2:63248373-63248392	Msa1261000:CDS	25.0%
!	TTCAAACAAAGATTCTTCTA+AGG	+	chr8_2:63248538-63248557	Msa1261000:CDS	25.0%
!	TCGATGAACAATTATATGAA+GGG	-	chr8_2:63248769-63248788	None:intergenic	25.0%
!	ATCGATGAACAATTATATGA+AGG	-	chr8_2:63248770-63248789	None:intergenic	25.0%
!	ATGAAATATATTTGTGCACA+CGG	-	chr8_2:63248888-63248907	None:intergenic	25.0%
!	AGATGTACAAAATTTCAACA+AGG	+	chr8_2:63248922-63248941	Msa1261000:intron	25.0%
!	TTACTTGGTTTAGACAAATT+TGG	+	chr8_2:63249034-63249053	Msa1261000:CDS	25.0%
!!!	ATGCACAAAAGTATTTCATA+AGG	+	chr8_2:63249125-63249144	Msa1261000:CDS	25.0%
!	TGTTAACACTAGTAATATCA+TGG	-	chr8_2:63249189-63249208	None:intergenic	25.0%
!	GATATTACTAGTGTTAACAA+TGG	+	chr8_2:63249190-63249209	Msa1261000:CDS	25.0%
!	AAAATAAAGTTTAGGAGAGT+TGG	+	chr8_2:63249532-63249551	Msa1261000:three_prime_UTR	25.0%
!!	AAGAAAAATCCTCTTTTATG+TGG	+	chr8_2:63249695-63249714	Msa1261000:three_prime_UTR	25.0%
!	AACTATTTATTAGCATCTCT+AGG	-	chr8_2:63249957-63249976	None:intergenic	25.0%
!	TGGTGAAATTAATTGAAGAT+GGG	-	chr8_2:63248217-63248236	None:intergenic	25.0%
!	ATGGTGAAATTAATTGAAGA+TGG	-	chr8_2:63248218-63248237	None:intergenic	25.0%
!	AAGAAGATTCAAAAGAACAA+TGG	+	chr8_2:63248372-63248391	Msa1261000:CDS	25.0%
!	AGAAGATTCAAAAGAACAAT+GGG	+	chr8_2:63248373-63248392	Msa1261000:CDS	25.0%
!	TTCAAACAAAGATTCTTCTA+AGG	+	chr8_2:63248538-63248557	Msa1261000:CDS	25.0%
!	TCGATGAACAATTATATGAA+GGG	-	chr8_2:63248769-63248788	None:intergenic	25.0%
!	ATCGATGAACAATTATATGA+AGG	-	chr8_2:63248770-63248789	None:intergenic	25.0%
!	ATGAAATATATTTGTGCACA+CGG	-	chr8_2:63248888-63248907	None:intergenic	25.0%
!	AGATGTACAAAATTTCAACA+AGG	+	chr8_2:63248922-63248941	Msa1261000:intron	25.0%
!	TTACTTGGTTTAGACAAATT+TGG	+	chr8_2:63249034-63249053	Msa1261000:CDS	25.0%
!!!	ATGCACAAAAGTATTTCATA+AGG	+	chr8_2:63249125-63249144	Msa1261000:CDS	25.0%
!	TGTTAACACTAGTAATATCA+TGG	-	chr8_2:63249189-63249208	None:intergenic	25.0%
!	GATATTACTAGTGTTAACAA+TGG	+	chr8_2:63249190-63249209	Msa1261000:CDS	25.0%
!	AAAATAAAGTTTAGGAGAGT+TGG	+	chr8_2:63249532-63249551	Msa1261000:three_prime_UTR	25.0%
!!	AAGAAAAATCCTCTTTTATG+TGG	+	chr8_2:63249695-63249714	Msa1261000:three_prime_UTR	25.0%
!	AACTATTTATTAGCATCTCT+AGG	-	chr8_2:63249957-63249976	None:intergenic	25.0%
!	AATGTAAGTCTTTTTGTGAG+TGG	-	chr8_2:63248181-63248200	None:intergenic	30.0%
	CAAAGTTTAGAGAATGAGAA+TGG	-	chr8_2:63248237-63248256	None:intergenic	30.0%
!!	GTTGGATTTCTTTGGAATTA+TGG	-	chr8_2:63248262-63248281	None:intergenic	30.0%
!!!	TTCTTTTTCAATCCAATGCA+TGG	-	chr8_2:63248357-63248376	None:intergenic	30.0%
!	ACTTGTTCCATACTTTTGTT+GGG	-	chr8_2:63248419-63248438	None:intergenic	30.0%
!	TGATCTTTTATCTGAACTTG+TGG	-	chr8_2:63248567-63248586	None:intergenic	30.0%
!!	TCAAGTTGTAATTTTGGAAG+TGG	+	chr8_2:63248584-63248603	Msa1261000:CDS	30.0%
!!	CAAGTTGTAATTTTGGAAGT+GGG	+	chr8_2:63248585-63248604	Msa1261000:CDS	30.0%
	CAAGGATTCATTTCAATTCA+AGG	+	chr8_2:63248940-63248959	Msa1261000:intron	30.0%
	GGTTTAGACAAATTTGGAAA+AGG	+	chr8_2:63249040-63249059	Msa1261000:CDS	30.0%
	ACAAATTTGGAAAAGGAGAT+TGG	+	chr8_2:63249047-63249066	Msa1261000:CDS	30.0%
	CAATGAATAGAGATAGAAGA+AGG	+	chr8_2:63249155-63249174	Msa1261000:CDS	30.0%
	CTTAACTTTCCACATAAAAG+AGG	-	chr8_2:63249707-63249726	None:intergenic	30.0%
	TAGTAGATTTCCTTATTAGG+TGG	+	chr8_2:63249833-63249852	Msa1261000:three_prime_UTR	30.0%
	ACATGACATGATCATAATCT+TGG	-	chr8_2:63249932-63249951	None:intergenic	30.0%
!	AATGTAAGTCTTTTTGTGAG+TGG	-	chr8_2:63248181-63248200	None:intergenic	30.0%
	CAAAGTTTAGAGAATGAGAA+TGG	-	chr8_2:63248237-63248256	None:intergenic	30.0%
!!	GTTGGATTTCTTTGGAATTA+TGG	-	chr8_2:63248262-63248281	None:intergenic	30.0%
!!!	TTCTTTTTCAATCCAATGCA+TGG	-	chr8_2:63248357-63248376	None:intergenic	30.0%
!	ACTTGTTCCATACTTTTGTT+GGG	-	chr8_2:63248419-63248438	None:intergenic	30.0%
!	TGATCTTTTATCTGAACTTG+TGG	-	chr8_2:63248567-63248586	None:intergenic	30.0%
!!	TCAAGTTGTAATTTTGGAAG+TGG	+	chr8_2:63248584-63248603	Msa1261000:CDS	30.0%
!!	CAAGTTGTAATTTTGGAAGT+GGG	+	chr8_2:63248585-63248604	Msa1261000:CDS	30.0%
	CAAGGATTCATTTCAATTCA+AGG	+	chr8_2:63248940-63248959	Msa1261000:intron	30.0%
	GGTTTAGACAAATTTGGAAA+AGG	+	chr8_2:63249040-63249059	Msa1261000:CDS	30.0%
	ACAAATTTGGAAAAGGAGAT+TGG	+	chr8_2:63249047-63249066	Msa1261000:CDS	30.0%
	CAATGAATAGAGATAGAAGA+AGG	+	chr8_2:63249155-63249174	Msa1261000:CDS	30.0%
	CTTAACTTTCCACATAAAAG+AGG	-	chr8_2:63249707-63249726	None:intergenic	30.0%
	TAGTAGATTTCCTTATTAGG+TGG	+	chr8_2:63249833-63249852	Msa1261000:three_prime_UTR	30.0%
	ACATGACATGATCATAATCT+TGG	-	chr8_2:63249932-63249951	None:intergenic	30.0%
	ACATGACATGGTAGTATAGA+GGG	-	chr8_2:63248147-63248166	None:intergenic	35.0%
	TACATGACATGGTAGTATAG+AGG	-	chr8_2:63248148-63248167	None:intergenic	35.0%
	GAATTTGTCTCTACATGACA+TGG	-	chr8_2:63248159-63248178	None:intergenic	35.0%
	ATTCCAAAGAAATCCAACCA+AGG	+	chr8_2:63248264-63248283	Msa1261000:five_prime_UTR	35.0%
	TTCCAAAGAAATCCAACCAA+GGG	+	chr8_2:63248265-63248284	Msa1261000:five_prime_UTR	35.0%
	TTCTTCTTCATAGCTCCAAA+TGG	-	chr8_2:63248312-63248331	None:intergenic	35.0%
!	CTTGTTCCATACTTTTGTTG+GGG	-	chr8_2:63248418-63248437	None:intergenic	35.0%
!	CACTTGTTCCATACTTTTGT+TGG	-	chr8_2:63248420-63248439	None:intergenic	35.0%
	GAAACAACATTACCAAGTGT+TGG	+	chr8_2:63248439-63248458	Msa1261000:CDS	35.0%
	AGATGATGTTAGTGCAATTG+AGG	+	chr8_2:63248463-63248482	Msa1261000:CDS	35.0%
!	TCTTTTATCTGAACTTGTGG+TGG	-	chr8_2:63248564-63248583	None:intergenic	35.0%
!!	AATTTTGGAAGTGGGTTTTC+TGG	+	chr8_2:63248593-63248612	Msa1261000:CDS	35.0%
	CATCATCATCAGAACAAGAA+AGG	+	chr8_2:63248672-63248691	Msa1261000:CDS	35.0%
	TCAGAACAAGAAAGGAGAAA+AGG	+	chr8_2:63248680-63248699	Msa1261000:CDS	35.0%
	AAAGGAGAAAAGGAATTCCA+TGG	+	chr8_2:63248690-63248709	Msa1261000:CDS	35.0%
!	GAGATTGGAGAAGTATATCA+AGG	+	chr8_2:63249062-63249081	Msa1261000:CDS	35.0%
	CAAGGAACTTTGTGATATCT+AGG	+	chr8_2:63249080-63249099	Msa1261000:CDS	35.0%
	AATCATCAAGCACCAATAAC+AGG	+	chr8_2:63249226-63249245	Msa1261000:CDS	35.0%
	TTACCATGTTGACCTGTTAT+TGG	-	chr8_2:63249241-63249260	None:intergenic	35.0%
	CACAATCCCTTCAAATACAA+CGG	+	chr8_2:63249261-63249280	Msa1261000:CDS	35.0%
	ACAATCCCTTCAAATACAAC+GGG	+	chr8_2:63249262-63249281	Msa1261000:CDS	35.0%
	CTAAACCCGTTGTATTTGAA+GGG	-	chr8_2:63249270-63249289	None:intergenic	35.0%
!	TAAGCAAGAGGTACAACATA+AGG	-	chr8_2:63249451-63249470	None:intergenic	35.0%
!	AGTTTAGGAGAGTTGGATTT+TGG	+	chr8_2:63249539-63249558	Msa1261000:three_prime_UTR	35.0%
	TAGATTTCCTTATTAGGTGG+TGG	+	chr8_2:63249836-63249855	Msa1261000:three_prime_UTR	35.0%
	AGATTTCCTTATTAGGTGGT+GGG	+	chr8_2:63249837-63249856	Msa1261000:three_prime_UTR	35.0%
	ACATGACATGGTAGTATAGA+GGG	-	chr8_2:63248147-63248166	None:intergenic	35.0%
	TACATGACATGGTAGTATAG+AGG	-	chr8_2:63248148-63248167	None:intergenic	35.0%
	GAATTTGTCTCTACATGACA+TGG	-	chr8_2:63248159-63248178	None:intergenic	35.0%
	ATTCCAAAGAAATCCAACCA+AGG	+	chr8_2:63248264-63248283	Msa1261000:five_prime_UTR	35.0%
	TTCCAAAGAAATCCAACCAA+GGG	+	chr8_2:63248265-63248284	Msa1261000:five_prime_UTR	35.0%
	TTCTTCTTCATAGCTCCAAA+TGG	-	chr8_2:63248312-63248331	None:intergenic	35.0%
!	CTTGTTCCATACTTTTGTTG+GGG	-	chr8_2:63248418-63248437	None:intergenic	35.0%
!	CACTTGTTCCATACTTTTGT+TGG	-	chr8_2:63248420-63248439	None:intergenic	35.0%
	GAAACAACATTACCAAGTGT+TGG	+	chr8_2:63248439-63248458	Msa1261000:CDS	35.0%
	AGATGATGTTAGTGCAATTG+AGG	+	chr8_2:63248463-63248482	Msa1261000:CDS	35.0%
!	TCTTTTATCTGAACTTGTGG+TGG	-	chr8_2:63248564-63248583	None:intergenic	35.0%
!!	AATTTTGGAAGTGGGTTTTC+TGG	+	chr8_2:63248593-63248612	Msa1261000:CDS	35.0%
	CATCATCATCAGAACAAGAA+AGG	+	chr8_2:63248672-63248691	Msa1261000:CDS	35.0%
	TCAGAACAAGAAAGGAGAAA+AGG	+	chr8_2:63248680-63248699	Msa1261000:CDS	35.0%
	AAAGGAGAAAAGGAATTCCA+TGG	+	chr8_2:63248690-63248709	Msa1261000:CDS	35.0%
!	GAGATTGGAGAAGTATATCA+AGG	+	chr8_2:63249062-63249081	Msa1261000:CDS	35.0%
	CAAGGAACTTTGTGATATCT+AGG	+	chr8_2:63249080-63249099	Msa1261000:CDS	35.0%
	AATCATCAAGCACCAATAAC+AGG	+	chr8_2:63249226-63249245	Msa1261000:CDS	35.0%
	TTACCATGTTGACCTGTTAT+TGG	-	chr8_2:63249241-63249260	None:intergenic	35.0%
	CACAATCCCTTCAAATACAA+CGG	+	chr8_2:63249261-63249280	Msa1261000:CDS	35.0%
	ACAATCCCTTCAAATACAAC+GGG	+	chr8_2:63249262-63249281	Msa1261000:CDS	35.0%
	CTAAACCCGTTGTATTTGAA+GGG	-	chr8_2:63249270-63249289	None:intergenic	35.0%
!	TAAGCAAGAGGTACAACATA+AGG	-	chr8_2:63249451-63249470	None:intergenic	35.0%
!	AGTTTAGGAGAGTTGGATTT+TGG	+	chr8_2:63249539-63249558	Msa1261000:three_prime_UTR	35.0%
	TAGATTTCCTTATTAGGTGG+TGG	+	chr8_2:63249836-63249855	Msa1261000:three_prime_UTR	35.0%
	AGATTTCCTTATTAGGTGGT+GGG	+	chr8_2:63249837-63249856	Msa1261000:three_prime_UTR	35.0%
	AGAATGCTATAGCCATGCAT+TGG	+	chr8_2:63248342-63248361	Msa1261000:CDS	40.0%
	ATCTGTCCCCAACAAAAGTA+TGG	+	chr8_2:63248409-63248428	Msa1261000:CDS	40.0%
	TAACATCATCTGCCAACACT+TGG	-	chr8_2:63248454-63248473	None:intergenic	40.0%
	GATGTTAGTGCAATTGAGGA+AGG	+	chr8_2:63248467-63248486	Msa1261000:CDS	40.0%
	ACCTTTACCACTACTATGGT+GGG	-	chr8_2:63248636-63248655	None:intergenic	40.0%
	ATCCACCTTTACCACTACTA+TGG	-	chr8_2:63248640-63248659	None:intergenic	40.0%
	CCTAAACCCGTTGTATTTGA+AGG	-	chr8_2:63249271-63249290	None:intergenic	40.0%
!	CCTTCAAATACAACGGGTTT+AGG	+	chr8_2:63249268-63249287	Msa1261000:CDS	40.0%
!	TGGATGATGATGAGGATGAT+GGG	-	chr8_2:63249428-63249447	None:intergenic	40.0%
!	ACATAAGGTGGATGATGATG+AGG	-	chr8_2:63249436-63249455	None:intergenic	40.0%
	AGTTGTCATTGGTGCATTGT+TGG	-	chr8_2:63249487-63249506	None:intergenic	40.0%
!	TTAGGAGAGTTGGATTTTGG+AGG	+	chr8_2:63249542-63249561	Msa1261000:three_prime_UTR	40.0%
	AGAATGCTATAGCCATGCAT+TGG	+	chr8_2:63248342-63248361	Msa1261000:CDS	40.0%
	ATCTGTCCCCAACAAAAGTA+TGG	+	chr8_2:63248409-63248428	Msa1261000:CDS	40.0%
	TAACATCATCTGCCAACACT+TGG	-	chr8_2:63248454-63248473	None:intergenic	40.0%
	GATGTTAGTGCAATTGAGGA+AGG	+	chr8_2:63248467-63248486	Msa1261000:CDS	40.0%
	ACCTTTACCACTACTATGGT+GGG	-	chr8_2:63248636-63248655	None:intergenic	40.0%
	ATCCACCTTTACCACTACTA+TGG	-	chr8_2:63248640-63248659	None:intergenic	40.0%
	CCTAAACCCGTTGTATTTGA+AGG	-	chr8_2:63249271-63249290	None:intergenic	40.0%
!	CCTTCAAATACAACGGGTTT+AGG	+	chr8_2:63249268-63249287	Msa1261000:CDS	40.0%
!	TGGATGATGATGAGGATGAT+GGG	-	chr8_2:63249428-63249447	None:intergenic	40.0%
!	ACATAAGGTGGATGATGATG+AGG	-	chr8_2:63249436-63249455	None:intergenic	40.0%
	AGTTGTCATTGGTGCATTGT+TGG	-	chr8_2:63249487-63249506	None:intergenic	40.0%
!	TTAGGAGAGTTGGATTTTGG+AGG	+	chr8_2:63249542-63249561	Msa1261000:three_prime_UTR	40.0%
	TGTCATCAAGTGGAGCCATT+TGG	+	chr8_2:63248294-63248313	Msa1261000:CDS	45.0%
!!!	GGAAGTGGGTTTTCTGGTTT+AGG	+	chr8_2:63248599-63248618	Msa1261000:CDS	45.0%
	ACCCACCATAGTAGTGGTAA+AGG	+	chr8_2:63248632-63248651	Msa1261000:CDS	45.0%
	CACCTTTACCACTACTATGG+TGG	-	chr8_2:63248637-63248656	None:intergenic	45.0%
	CACCATAGTAGTGGTAAAGG+TGG	+	chr8_2:63248635-63248654	Msa1261000:CDS	45.0%
!!	GTGGTAAAGGTGGATTGTCA+AGG	+	chr8_2:63248645-63248664	Msa1261000:CDS	45.0%
	CTATGCTCTTCTTCTGTCCA+TGG	-	chr8_2:63248710-63248729	None:intergenic	45.0%
!	CATGGACAGAAGAAGAGCAT+AGG	+	chr8_2:63248708-63248727	Msa1261000:CDS	45.0%
!!	GCATGACTTGCTACTTGTGT+TGG	-	chr8_2:63249109-63249128	None:intergenic	45.0%
	GCACCAATAACAGGTCAACA+TGG	+	chr8_2:63249235-63249254	Msa1261000:CDS	45.0%
	TACATTCCTAAACCACCAGG+AGG	-	chr8_2:63249328-63249347	None:intergenic	45.0%
	CCATACATTCCTAAACCACC+AGG	-	chr8_2:63249331-63249350	None:intergenic	45.0%
	CCTGGTGGTTTAGGAATGTA+TGG	+	chr8_2:63249328-63249347	Msa1261000:CDS	45.0%
	CTCATATGGCATCTGCAGTT+GGG	+	chr8_2:63249386-63249405	Msa1261000:CDS	45.0%
!	GGATGATGATGAGGATGATG+GGG	-	chr8_2:63249427-63249446	None:intergenic	45.0%
!	GTGGATGATGATGAGGATGA+TGG	-	chr8_2:63249429-63249448	None:intergenic	45.0%
!	GCAAGAGGTACAACATAAGG+TGG	-	chr8_2:63249448-63249467	None:intergenic	45.0%
	TGTACCTCTTGCTTACCCTA+TGG	+	chr8_2:63249456-63249475	Msa1261000:CDS	45.0%
	TGTCATTGGTGCATTGTTGG+AGG	-	chr8_2:63249484-63249503	None:intergenic	45.0%
!	CCAACATTGGTTGGTTCAGT+TGG	-	chr8_2:63249628-63249647	None:intergenic	45.0%
	CCAACTGAACCAACCAATGT+TGG	+	chr8_2:63249625-63249644	Msa1261000:three_prime_UTR	45.0%
	AAGAGGAGTTGAGCCAACAT+TGG	-	chr8_2:63249641-63249660	None:intergenic	45.0%
	TGTCATCAAGTGGAGCCATT+TGG	+	chr8_2:63248294-63248313	Msa1261000:CDS	45.0%
!!!	GGAAGTGGGTTTTCTGGTTT+AGG	+	chr8_2:63248599-63248618	Msa1261000:CDS	45.0%
	ACCCACCATAGTAGTGGTAA+AGG	+	chr8_2:63248632-63248651	Msa1261000:CDS	45.0%
	CACCTTTACCACTACTATGG+TGG	-	chr8_2:63248637-63248656	None:intergenic	45.0%
	CACCATAGTAGTGGTAAAGG+TGG	+	chr8_2:63248635-63248654	Msa1261000:CDS	45.0%
!!	GTGGTAAAGGTGGATTGTCA+AGG	+	chr8_2:63248645-63248664	Msa1261000:CDS	45.0%
	CTATGCTCTTCTTCTGTCCA+TGG	-	chr8_2:63248710-63248729	None:intergenic	45.0%
!	CATGGACAGAAGAAGAGCAT+AGG	+	chr8_2:63248708-63248727	Msa1261000:CDS	45.0%
!!	GCATGACTTGCTACTTGTGT+TGG	-	chr8_2:63249109-63249128	None:intergenic	45.0%
	GCACCAATAACAGGTCAACA+TGG	+	chr8_2:63249235-63249254	Msa1261000:CDS	45.0%
	TACATTCCTAAACCACCAGG+AGG	-	chr8_2:63249328-63249347	None:intergenic	45.0%
	CCATACATTCCTAAACCACC+AGG	-	chr8_2:63249331-63249350	None:intergenic	45.0%
	CCTGGTGGTTTAGGAATGTA+TGG	+	chr8_2:63249328-63249347	Msa1261000:CDS	45.0%
	CTCATATGGCATCTGCAGTT+GGG	+	chr8_2:63249386-63249405	Msa1261000:CDS	45.0%
!	GGATGATGATGAGGATGATG+GGG	-	chr8_2:63249427-63249446	None:intergenic	45.0%
!	GTGGATGATGATGAGGATGA+TGG	-	chr8_2:63249429-63249448	None:intergenic	45.0%
!	GCAAGAGGTACAACATAAGG+TGG	-	chr8_2:63249448-63249467	None:intergenic	45.0%
	TGTACCTCTTGCTTACCCTA+TGG	+	chr8_2:63249456-63249475	Msa1261000:CDS	45.0%
	TGTCATTGGTGCATTGTTGG+AGG	-	chr8_2:63249484-63249503	None:intergenic	45.0%
!	CCAACATTGGTTGGTTCAGT+TGG	-	chr8_2:63249628-63249647	None:intergenic	45.0%
	CCAACTGAACCAACCAATGT+TGG	+	chr8_2:63249625-63249644	Msa1261000:three_prime_UTR	45.0%
	AAGAGGAGTTGAGCCAACAT+TGG	-	chr8_2:63249641-63249660	None:intergenic	45.0%
	GGCCCTTGGTTGGATTTCTT+TGG	-	chr8_2:63248270-63248289	None:intergenic	50.0%
	ATGACATGTTGGCCCTTGGT+TGG	-	chr8_2:63248280-63248299	None:intergenic	50.0%
!	CTTGATGACATGTTGGCCCT+TGG	-	chr8_2:63248284-63248303	None:intergenic	50.0%
	AGGGCCAACATGTCATCAAG+TGG	+	chr8_2:63248284-63248303	Msa1261000:exon	50.0%
!	GGCTCCACTTGATGACATGT+TGG	-	chr8_2:63248291-63248310	None:intergenic	50.0%
	GACTCTACCCACCATAGTAG+TGG	+	chr8_2:63248626-63248645	Msa1261000:CDS	50.0%
	AAACCACCAGGAGGATGATG+AGG	-	chr8_2:63249319-63249338	None:intergenic	50.0%
	CATCATCCTCCTGGTGGTTT+AGG	+	chr8_2:63249319-63249338	Msa1261000:CDS	50.0%
!	GGAATGTATGGAGCACCAGT+AGG	+	chr8_2:63249340-63249359	Msa1261000:CDS	50.0%
	GCAACAACAGGATGTCCTAC+TGG	-	chr8_2:63249358-63249377	None:intergenic	50.0%
	TGTTGCTCCTCCTGCTCATA+TGG	+	chr8_2:63249372-63249391	Msa1261000:CDS	50.0%
	ACTGCAGATGCCATATGAGC+AGG	-	chr8_2:63249385-63249404	None:intergenic	50.0%
	GCTCATATGGCATCTGCAGT+TGG	+	chr8_2:63249385-63249404	Msa1261000:CDS	50.0%
!!	GGATGATGGGGAAGCATAAC+AGG	-	chr8_2:63249415-63249434	None:intergenic	50.0%
!!	GCATTGTTGGAGGTGCCATA+GGG	-	chr8_2:63249474-63249493	None:intergenic	50.0%
!!	TGCATTGTTGGAGGTGCCAT+AGG	-	chr8_2:63249475-63249494	None:intergenic	50.0%
!!	TTGGATTTTGGAGGCTCGTC+AGG	+	chr8_2:63249551-63249570	Msa1261000:three_prime_UTR	50.0%
!!	TGGATTTTGGAGGCTCGTCA+GGG	+	chr8_2:63249552-63249571	Msa1261000:three_prime_UTR	50.0%
	GACTCAGAGTGTAGTGACAG+AGG	+	chr8_2:63249591-63249610	Msa1261000:three_prime_UTR	50.0%
	GGAGTTGAGCCAACATTGGT+TGG	-	chr8_2:63249637-63249656	None:intergenic	50.0%
	CCAAGACCCACCACCTAATA+AGG	-	chr8_2:63249846-63249865	None:intergenic	50.0%
!!	CCTTATTAGGTGGTGGGTCT+TGG	+	chr8_2:63249843-63249862	Msa1261000:three_prime_UTR	50.0%
	GGCCCTTGGTTGGATTTCTT+TGG	-	chr8_2:63248270-63248289	None:intergenic	50.0%
	ATGACATGTTGGCCCTTGGT+TGG	-	chr8_2:63248280-63248299	None:intergenic	50.0%
!	CTTGATGACATGTTGGCCCT+TGG	-	chr8_2:63248284-63248303	None:intergenic	50.0%
	AGGGCCAACATGTCATCAAG+TGG	+	chr8_2:63248284-63248303	Msa1261000:exon	50.0%
!	GGCTCCACTTGATGACATGT+TGG	-	chr8_2:63248291-63248310	None:intergenic	50.0%
	GACTCTACCCACCATAGTAG+TGG	+	chr8_2:63248626-63248645	Msa1261000:CDS	50.0%
	AAACCACCAGGAGGATGATG+AGG	-	chr8_2:63249319-63249338	None:intergenic	50.0%
	CATCATCCTCCTGGTGGTTT+AGG	+	chr8_2:63249319-63249338	Msa1261000:CDS	50.0%
!	GGAATGTATGGAGCACCAGT+AGG	+	chr8_2:63249340-63249359	Msa1261000:CDS	50.0%
	GCAACAACAGGATGTCCTAC+TGG	-	chr8_2:63249358-63249377	None:intergenic	50.0%
	TGTTGCTCCTCCTGCTCATA+TGG	+	chr8_2:63249372-63249391	Msa1261000:CDS	50.0%
	ACTGCAGATGCCATATGAGC+AGG	-	chr8_2:63249385-63249404	None:intergenic	50.0%
	GCTCATATGGCATCTGCAGT+TGG	+	chr8_2:63249385-63249404	Msa1261000:CDS	50.0%
!!	GGATGATGGGGAAGCATAAC+AGG	-	chr8_2:63249415-63249434	None:intergenic	50.0%
!!	GCATTGTTGGAGGTGCCATA+GGG	-	chr8_2:63249474-63249493	None:intergenic	50.0%
!!	TGCATTGTTGGAGGTGCCAT+AGG	-	chr8_2:63249475-63249494	None:intergenic	50.0%
!!	TTGGATTTTGGAGGCTCGTC+AGG	+	chr8_2:63249551-63249570	Msa1261000:three_prime_UTR	50.0%
!!	TGGATTTTGGAGGCTCGTCA+GGG	+	chr8_2:63249552-63249571	Msa1261000:three_prime_UTR	50.0%
	GACTCAGAGTGTAGTGACAG+AGG	+	chr8_2:63249591-63249610	Msa1261000:three_prime_UTR	50.0%
	GGAGTTGAGCCAACATTGGT+TGG	-	chr8_2:63249637-63249656	None:intergenic	50.0%
	CCAAGACCCACCACCTAATA+AGG	-	chr8_2:63249846-63249865	None:intergenic	50.0%
!!	CCTTATTAGGTGGTGGGTCT+TGG	+	chr8_2:63249843-63249862	Msa1261000:three_prime_UTR	50.0%
	GTTCAGCCTCATCATCCTCC+TGG	+	chr8_2:63249310-63249329	Msa1261000:CDS	55.0%
	TGAGCAGGAGGAGCAACAAC+AGG	-	chr8_2:63249370-63249389	None:intergenic	55.0%
	GCAGATGCCATATGAGCAGG+AGG	-	chr8_2:63249382-63249401	None:intergenic	55.0%
!	GGTGCCATAGGGTAAGCAAG+AGG	-	chr8_2:63249463-63249482	None:intergenic	55.0%
!!	TTTTGGAGGCTCGTCAGGGT+CGG	+	chr8_2:63249556-63249575	Msa1261000:three_prime_UTR	55.0%
	GTTCAGCCTCATCATCCTCC+TGG	+	chr8_2:63249310-63249329	Msa1261000:CDS	55.0%
	TGAGCAGGAGGAGCAACAAC+AGG	-	chr8_2:63249370-63249389	None:intergenic	55.0%
	GCAGATGCCATATGAGCAGG+AGG	-	chr8_2:63249382-63249401	None:intergenic	55.0%
!	GGTGCCATAGGGTAAGCAAG+AGG	-	chr8_2:63249463-63249482	None:intergenic	55.0%
!!	TTTTGGAGGCTCGTCAGGGT+CGG	+	chr8_2:63249556-63249575	Msa1261000:three_prime_UTR	55.0%
	CAGCCTCATCATCCTCCTGG+TGG	+	chr8_2:63249313-63249332	Msa1261000:CDS	60.0%
	TTTGGAGGCTCGTCAGGGTC+GGG	+	chr8_2:63249557-63249576	Msa1261000:three_prime_UTR	60.0%
	CAGCCTCATCATCCTCCTGG+TGG	+	chr8_2:63249313-63249332	Msa1261000:CDS	60.0%
	TTTGGAGGCTCGTCAGGGTC+GGG	+	chr8_2:63249557-63249576	Msa1261000:three_prime_UTR	60.0%
	TTGGAGGCTCGTCAGGGTCG+GGG	+	chr8_2:63249558-63249577	Msa1261000:three_prime_UTR	65.0%
	TTGGAGGCTCGTCAGGGTCG+GGG	+	chr8_2:63249558-63249577	Msa1261000:three_prime_UTR	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	63248138	63249987	63248138	ID=Msa1261000;Name=Msa1261000
chr8_2	mRNA	63248138	63249987	63248138	ID=Msa1261000-mRNA-1;Parent=Msa1261000;Name=Msa1261000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=155\|1\|1\|1\|1\|1\|2\|486\|303
chr8_2	exon	63248138	63248729	63248138	ID=Msa1261000-mRNA-1:exon:20186;Parent=Msa1261000-mRNA-1
chr8_2	exon	63249027	63249987	63249027	ID=Msa1261000-mRNA-1:exon:20187;Parent=Msa1261000-mRNA-1
chr8_2	five_prime_UTR	63248138	63248292	63248138	ID=Msa1261000-mRNA-1:five_prime_utr;Parent=Msa1261000-mRNA-1
chr8_2	CDS	63248293	63248729	63248293	ID=Msa1261000-mRNA-1:cds;Parent=Msa1261000-mRNA-1
chr8_2	CDS	63249027	63249501	63249027	ID=Msa1261000-mRNA-1:cds;Parent=Msa1261000-mRNA-1
chr8_2	three_prime_UTR	63249502	63249987	63249502	ID=Msa1261000-mRNA-1:three_prime_utr;Parent=Msa1261000-mRNA-1

Gene Sequence

>Msa1261000

ATGTCATCAAGTGGAGCCATTTGGAGCTATGAAGAAGAAAAAGCATTTGAGAATGCTATAGCCATGCATTGGATTGAAAAAGAAGATTCAAAAGAACAATGGGAAAAGATTGCTTCATCTGTCCCCAACAAAAGTATGGAACAAGTGAAACAACATTACCAAGTGTTGGCAGATGATGTTAGTGCAATTGAGGAAGGTCATGTATCATTGCCAAATTATGCTAATGAATTAGATACTATTAATTCTTCAAACAAAGATTCTTCTAAGGCCACCACAAGTTCAGATAAAAGATCAAGTTGTAATTTTGGAAGTGGGTTTTCTGGTTTAGGACATGACTCTACCCACCATAGTAGTGGTAAAGGTGGATTGTCAAGGTCATCATCATCATCAGAACAAGAAAGGAGAAAAGGAATTCCATGGACAGAAGAAGAGCATAGGTTATTTTTACTTGGTTTAGACAAATTTGGAAAAGGAGATTGGAGAAGTATATCAAGGAACTTTGTGATATCTAGGACACCAACACAAGTAGCAAGTCATGCACAAAAGTATTTCATAAGGTTGAATTCAATGAATAGAGATAGAAGAAGGTCTAGTATCCATGATATTACTAGTGTTAACAATGGAGATGTAGCTAGTAATCATCAAGCACCAATAACAGGTCAACATGGTAACACAATCCCTTCAAATACAACGGGTTTAGGACAATCAATGAAGCATAGAGTTCAGCCTCATCATCCTCCTGGTGGTTTAGGAATGTATGGAGCACCAGTAGGACATCCTGTTGTTGCTCCTCCTGCTCATATGGCATCTGCAGTTGGGACACCTGTTATGCTTCCCCATCATCCTCATCATCATCCACCTTATGTTGTACCTCTTGCTTACCCTATGGCACCTCCAACAATGCACCAATGA

Protein sequence

>Msa1261000

MSSSGAIWSYEEEKAFENAIAMHWIEKEDSKEQWEKIASSVPNKSMEQVKQHYQVLADDVSAIEEGHVSLPNYANELDTINSSNKDSSKATTSSDKRSSCNFGSGFSGLGHDSTHHSSGKGGLSRSSSSSEQERRKGIPWTEEEHRLFLLGLDKFGKGDWRSISRNFVISRTPTQVASHAQKYFIRLNSMNRDRRRSSIHDITSVNNGDVASNHQAPITGQHGNTIPSNTTGLGQSMKHRVQPHHPPGGLGMYGAPVGHPVVAPPAHMASAVGTPVMLPHHPHHHPPYVVPLAYPMAPPTMHQ*