Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748130 | XP_003621433.2 | 90.947 | 972 | 68 | 4 | 1 | 962 | 1 | 962 | 0.0 | 1774 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748130 | sp|Q94A41|AMY3_ARATH | 47.857 | 420 | 187 | 11 | 554 | 959 | 483 | 884 | 1.93e-116 | 381 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007230 | Msa0748130 | 0.855359 | 6.652305e-62 | -8.615850e-47 |
| Msa0008330 | Msa0748130 | 0.818634 | 1.769204e-52 | -8.615850e-47 |
| Msa0011850 | Msa0748130 | 0.812376 | 4.373328e-51 | -8.615850e-47 |
| Msa0017120 | Msa0748130 | 0.801716 | 7.894802e-49 | -8.615850e-47 |
| Msa0018220 | Msa0748130 | 0.801932 | 7.130490e-49 | -8.615850e-47 |
| Msa0030000 | Msa0748130 | 0.824975 | 6.032713e-54 | -8.615850e-47 |
| Msa0032040 | Msa0748130 | 0.801233 | 9.919950e-49 | -8.615850e-47 |
| Msa0033600 | Msa0748130 | 0.809834 | 1.555127e-50 | -8.615850e-47 |
| Msa0033650 | Msa0748130 | 0.809535 | 1.803929e-50 | -8.615850e-47 |
| Msa0035090 | Msa0748130 | 0.842608 | 2.330477e-58 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748130 | MtrunA17_Chr7g0218861 | 90.947 | 972 | 68 | 4 | 1 | 962 | 1 | 962 | 0.0 | 1774 |
| Msa0748130 | MtrunA17_Chr8g0362571 | 48.418 | 411 | 183 | 9 | 562 | 959 | 514 | 908 | 5.91e-118 | 383 |
| Msa0748130 | MtrunA17_Chr1g0157251 | 43.928 | 387 | 187 | 9 | 566 | 937 | 43 | 414 | 9.42e-100 | 319 |
| Msa0748130 | MtrunA17_Chr1g0153391 | 40.098 | 409 | 214 | 11 | 569 | 959 | 25 | 420 | 1.24e-83 | 276 |
| Msa0748130 | MtrunA17_Chr1g0153421 | 46.995 | 183 | 90 | 4 | 778 | 959 | 42 | 218 | 5.67e-51 | 179 |
| Msa0748130 | MtrunA17_Chr1g0153411 | 37.500 | 136 | 74 | 5 | 569 | 694 | 25 | 159 | 9.94e-22 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748130 | AT1G69830.1 | 47.857 | 420 | 187 | 11 | 554 | 959 | 483 | 884 | 1.97e-117 | 381 |
| Msa0748130 | AT1G76130.2 | 40.144 | 416 | 199 | 7 | 566 | 959 | 24 | 411 | 2.63e-99 | 318 |
| Msa0748130 | AT1G76130.1 | 40.144 | 416 | 199 | 7 | 566 | 959 | 24 | 411 | 2.63e-99 | 318 |
| Msa0748130 | AT4G25000.1 | 36.735 | 392 | 217 | 9 | 558 | 930 | 16 | 395 | 3.37e-71 | 243 |
Find 247 sgRNAs with CRISPR-Local
Find 1050 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGGTGCCTTGGAACAA+AGG | 0.551206 | 5_2:+1268821 | None:intergenic |
| AAAATGTTCCAAACACCATT+TGG | 0.354047 | 5_2:+1262142 | None:intergenic |
| AAACAGGTTACATACTTGTG+TGG | 0.655241 | 5_2:+1258035 | None:intergenic |
| AAAGGGTGCCTTGGAACAAA+GGG | 0.576112 | 5_2:+1268822 | None:intergenic |
| AAATAGATGCTCATCGGCAA+CGG | 0.583800 | 5_2:-1258709 | Msa0748130:intron |
| AAATGCAGATGAAGTACCAC+CGG | 0.620620 | 5_2:+1258664 | None:intergenic |
| AAATGCCTTCACCTGTTGTC+TGG | 0.377850 | 5_2:+1267110 | None:intergenic |
| AAATGGTCATAAAATATGAC+AGG | 0.559902 | 5_2:+1255465 | None:intergenic |
| AACAATTGAGAAGAAGCGGA+AGG | 0.639173 | 5_2:-1266443 | Msa0748130:CDS |
| AACATCGAAGTTCAGATCCT+TGG | 0.434053 | 5_2:-1266554 | Msa0748130:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTCATGATTCAACTTTCTC+TGG | - | chr5_2:1254697-1254716 | Msa0748130:three_prime_UTR | 30.0% | |
| GATTCTGCATATTTCAATCA+AGG | - | chr5_2:1254867-1254886 | Msa0748130:three_prime_UTR | 30.0% | |
| TATAGATGATGAACCTATAG+TGG | - | chr5_2:1255242-1255261 | Msa0748130:CDS | 30.0% | |
| CTGATTGAATTTGTAGTGTT+TGG | - | chr5_2:1255282-1255301 | Msa0748130:CDS | 30.0% | |
| GTAGTGTTTGGTAAAATTAG+TGG | - | chr5_2:1255294-1255313 | Msa0748130:CDS | 30.0% | |
| GAAGAAATGACATTACGATA+TGG | - | chr5_2:1255497-1255516 | Msa0748130:intron | 30.0% | |
| AGATGACTTATGTAATGTCA+TGG | - | chr5_2:1255734-1255753 | Msa0748130:intron | 30.0% | |
| GATGACTTATGTAATGTCAT+GGG | - | chr5_2:1255735-1255754 | Msa0748130:intron | 30.0% | |
| AGTTTATGGTAAAATCGATG+TGG | - | chr5_2:1255909-1255928 | Msa0748130:intron | 30.0% | |
| ACAAAGTTGAAGATATTCCA+CGG | + | chr5_2:1256151-1256170 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 1254580 | 1270386 | 1254580 | ID=Msa0748130;Name=Msa0748130 |
| chr5_2 | mRNA | 1254580 | 1270386 | 1254580 | ID=Msa0748130-mRNA-1;Parent=Msa0748130;Name=Msa0748130-mRNA-1;_AED=0.11;_eAED=0.11;_QI=394|0.86|0.93|0.93|0.86|0.81|16|635|962 |
| chr5_2 | exon | 1254580 | 1255487 | 1254580 | ID=Msa0748130-mRNA-1:exon:314;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1255669 | 1255737 | 1255669 | ID=Msa0748130-mRNA-1:exon:313;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1258050 | 1258184 | 1258050 | ID=Msa0748130-mRNA-1:exon:312;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1258649 | 1258725 | 1258649 | ID=Msa0748130-mRNA-1:exon:311;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1259543 | 1259669 | 1259543 | ID=Msa0748130-mRNA-1:exon:310;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1261338 | 1261453 | 1261338 | ID=Msa0748130-mRNA-1:exon:309;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1262062 | 1262167 | 1262062 | ID=Msa0748130-mRNA-1:exon:308;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1263139 | 1263183 | 1263139 | ID=Msa0748130-mRNA-1:exon:307;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1263671 | 1263768 | 1263671 | ID=Msa0748130-mRNA-1:exon:306;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1265295 | 1265427 | 1265295 | ID=Msa0748130-mRNA-1:exon:305;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1266235 | 1266266 | 1266235 | ID=Msa0748130-mRNA-1:exon:304;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1266377 | 1267874 | 1266377 | ID=Msa0748130-mRNA-1:exon:303;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1268211 | 1268271 | 1268211 | ID=Msa0748130-mRNA-1:exon:302;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1268783 | 1268908 | 1268783 | ID=Msa0748130-mRNA-1:exon:301;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1269827 | 1269904 | 1269827 | ID=Msa0748130-mRNA-1:exon:300;Parent=Msa0748130-mRNA-1 |
| chr5_2 | exon | 1270078 | 1270386 | 1270078 | ID=Msa0748130-mRNA-1:exon:299;Parent=Msa0748130-mRNA-1 |
| chr5_2 | five_prime_UTR | 1270078 | 1270386 | 1270078 | ID=Msa0748130-mRNA-1:five_prime_utr;Parent=Msa0748130-mRNA-1 |
| chr5_2 | five_prime_UTR | 1269827 | 1269904 | 1269827 | ID=Msa0748130-mRNA-1:five_prime_utr;Parent=Msa0748130-mRNA-1 |
| chr5_2 | five_prime_UTR | 1268902 | 1268908 | 1268902 | ID=Msa0748130-mRNA-1:five_prime_utr;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1268783 | 1268901 | 1268783 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1268211 | 1268271 | 1268211 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1266377 | 1267874 | 1266377 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1266235 | 1266266 | 1266235 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1265295 | 1265427 | 1265295 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1263671 | 1263768 | 1263671 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1263139 | 1263183 | 1263139 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1262062 | 1262167 | 1262062 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1261338 | 1261453 | 1261338 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1259543 | 1259669 | 1259543 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1258649 | 1258725 | 1258649 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1258050 | 1258184 | 1258050 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1255669 | 1255737 | 1255669 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | CDS | 1255215 | 1255487 | 1255215 | ID=Msa0748130-mRNA-1:cds;Parent=Msa0748130-mRNA-1 |
| chr5_2 | three_prime_UTR | 1254580 | 1255214 | 1254580 | ID=Msa0748130-mRNA-1:three_prime_utr;Parent=Msa0748130-mRNA-1 |
| Gene Sequence |
>Msa0748130 ATGGGAGCTGCTGTGTTGCCTGATACAGCAGTTTTGGGAGGAGTTCTTAAACGCTATCCCTTTGTTCCAAGGCACCCTTTTACCCGTGGAATATCTTCAACTTTGTCAGTAAGAAACAGAAGTTATTCCTTTGCTGACAAGAGAATCTTCAAATCAGCACACATAGTTTTTTCACACAATAATGGGGATGACATGTTTACAGATATAGTTGTTGATCAAGATTTAGGGAAAAATGAAGTTCTGGGAATAGAAGATGAATTAATAGCAGCCAAGAAATCTCTTTCAGAAGCACAAGACAGACAAGAGGCTATAGAGAAGGAGAGAGATCAATTGCTTGAAGAACTTGCACGCTCTGAGGCTAGAAAACAGGAATACATCGCCGCCATTTTACACGACAAAGAATTAGCTATAAGAGAGCTTGAGGCTGCCAAATCTCTTTTCCAGAAAAATCTGGAGGAATCTGTTGAAGAGAAGTTCTCGCTGCAATCTAAGCTGGTCCTTGCTAAAACAGATGCTGTTGATCTTGCAGTGCAGGTTGAAAAGTTAGCAGAGGCTGCTTTCCAGCAGGCAACTTCTCATATACTACAAGATGCTCAATTCAGAATTTCATCTGCTGAAACCACAGCCGCTGATGCAGCTCATCAGATCGAAAAGCAAATTAAGGATGCAACTGAGGGTACAATATCGTCCATTGTGGAGAAGTCAAAACATGCTATAGAAAGGGCTTTAGCTGTGGCAGAGGAAGCAGGTGAACATGCAAAGGAAGCCATGGAAACATTTATTGATGGTACATCTCCATTTACGGAAATTACCTCTGTCCAGGTAGAAAATATCAAGTTACAAGGAATGTTAAGTGATTTAGAATCTCAGATGATGGTTGCGAGAAATGAGGTTGCTAGACTGAACATAGAATTGGAGCACACCAGACAACAGGTGAAGGCATTTGAACAAAGAGCCATTGATGCGGAGAAAGCTTTGCTTGATCTTCAAGAGTCACACAGGAAAACCACTTTACAGCAAGAGGAGGAGATGAAGTCATTAATGGAGAAAATGAGAAAAGATGTGGCAGACAAGACGAAGGCAATTTCCAAGGCTTTTAAGACTGACTTGAAAAACATAAAGGCTACTATTGAAGCTTCAAAAGAAGTAGTCGTCAGTAAAGATAATGCCTACCTCAGAAGATGTGCAGCACTGCAGAGGTCATTGATGGCATCAGAAGATGCTCTGAAGATGTGGAAACAAAGAGCTGAAATGGCTGAAACATTGCTAATGAAAGAAAGAAAACTAGATGTAGAAGATGAAGATTCAATATATGCTGTTAATGGTGGGAGGATAGATCTTTTGACAGATGTTGATTCTCAAAAGTGGAAACTACTGAGTGACGGTCCTCGTAGAGATATACCTCAGTGGATGGCAAGAAGGATTAAGGCTGTCATTCCCAAATTTCCACCAAAAAAGACTGATGTAGCTGAAGCATTAACATCGAAGTTCAGATCCTTGGAGTTGCCTAAAGCCGATGAAGTATGGTCTATTGCTCGAGAAAAGCCGAAGGAAGGGGATGCTTTAATTGAACATGTTTTCGAAAGAGAAACAATTGAGAAGAAGCGGAAGGCCTTGGAGCGTGCTCTTCAACGGAAGACTATAAAGTGGGAGAAAGCCCCTGAACAAAAAATACTAGAGCCAGGAACAGGAACAGGACGAGAGATTGTGTTTCAAGCTTTTAACTGGGAGAGCTGGAGAAGGCAATGGTACCAGGAGTTGGCATCTAAAGCTGCTGATTTATCTAAATGTGGGGTGACTGCAGTGTGGCTGCCGCCACCAACAGAATCTGTTGCTGCTCAAGGTTACATGCCTTCTGATCTTTACAACTTGAATTCATCCTATGGTTCTGTGGAAGAACTTAAATATTGTATAGAGGAATTGCATTCTCACGATCTGCTGGCCTTGGGAGATGTTGTACTTAACCATCGATGTGCACATAAACAGAGTCCAAATGGTGTTTGGAACATTTTCGGTGGCAAGCTAGCATGGGGACCTGAAGCAATTGTATGCGATGATCCACATTTTCAGGGGCGTGGAAATCCTTCAAGTGAGGCTTCTCAGGCACATATGTTAAAGAATATATCGAAGCATCAAATCCTGTATTTGCCATTGGAGAATATTGGGACAGCTTGTCTTATGAACATGGAAGTTTGTGCTACAACCAAGATCTGCCCTGAAATCTCATGTGAATGTGAAAAAAAATCTTCCGGTGTTGGCTTTTGTTTAGTGCATGATGCACAGTTGTTGGAGCATTTAAGAGAGGTCCAGTTCTGTTGCCTGCGCGATGCGCGTGATGCTCATCGGCAACGGATTGTTAATTGGATCAATGCGACCGGTGGTACTTCATCTGCATTTGATATAACTACAAAGGGGATACTTCATTCCGCTTTGCACAATGAATATTGGAGAATGATAGACCCTCAAGGGAAACCAACAGGAGTTATGGGATGGTGGCCTTCTCGTGCTGTCACATTTCTAGAGAACCATGATACAGGGTCCACACAAGGCCACTGGCCATTTCCACGAGATAAGCTGATGCAGGGATATGCATATATCTTGACTCATCCTGGAACTCCTGTCATATTTTATGACCATTTCTATGATTTTGGCATCCATGATGTAATAACTGAGTTGATTGAGGCGCGGAGACGGGCTGGGATCCATTGTCGGAGCTCTATCAAGATATACAATGCAAACAATGAAGGATACGTAGCTCAAGTTGGTGATTCACTAGTAATGAAGCTTGGGCAGTTTGATTGGAATCCTTCAAAGGAGAACCGTTTGGAAGGGAGCTGGCAGAAGTTCGTTGACAAAGGATCAGATTACCAAGTTTGGTTGAGACAATAA |
| Protein sequence |
>Msa0748130 MGAAVLPDTAVLGGVLKRYPFVPRHPFTRGISSTLSVRNRSYSFADKRIFKSAHIVFSHNNGDDMFTDIVVDQDLGKNEVLGIEDELIAAKKSLSEAQDRQEAIEKERDQLLEELARSEARKQEYIAAILHDKELAIRELEAAKSLFQKNLEESVEEKFSLQSKLVLAKTDAVDLAVQVEKLAEAAFQQATSHILQDAQFRISSAETTAADAAHQIEKQIKDATEGTISSIVEKSKHAIERALAVAEEAGEHAKEAMETFIDGTSPFTEITSVQVENIKLQGMLSDLESQMMVARNEVARLNIELEHTRQQVKAFEQRAIDAEKALLDLQESHRKTTLQQEEEMKSLMEKMRKDVADKTKAISKAFKTDLKNIKATIEASKEVVVSKDNAYLRRCAALQRSLMASEDALKMWKQRAEMAETLLMKERKLDVEDEDSIYAVNGGRIDLLTDVDSQKWKLLSDGPRRDIPQWMARRIKAVIPKFPPKKTDVAEALTSKFRSLELPKADEVWSIAREKPKEGDALIEHVFERETIEKKRKALERALQRKTIKWEKAPEQKILEPGTGTGREIVFQAFNWESWRRQWYQELASKAADLSKCGVTAVWLPPPTESVAAQGYMPSDLYNLNSSYGSVEELKYCIEELHSHDLLALGDVVLNHRCAHKQSPNGVWNIFGGKLAWGPEAIVCDDPHFQGRGNPSSEASQAHMLKNISKHQILYLPLENIGTACLMNMEVCATTKICPEISCECEKKSSGVGFCLVHDAQLLEHLREVQFCCLRDARDAHRQRIVNWINATGGTSSAFDITTKGILHSALHNEYWRMIDPQGKPTGVMGWWPSRAVTFLENHDTGSTQGHWPFPRDKLMQGYAYILTHPGTPVIFYDHFYDFGIHDVITELIEARRRAGIHCRSSIKIYNANNEGYVAQVGDSLVMKLGQFDWNPSKENRLEGSWQKFVDKGSDYQVWLRQ* |
