Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27008.t1 | AUS45886.1 | 88 | 117 | 6 | 1 | 2 | 110 | 3 | 119 | 4.70E-48 | 200.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27008.t1 | Q43636 | 66.7 | 117 | 30 | 2 | 2 | 110 | 3 | 118 | 7.2e-37 | 154.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27008.t1 | A0A2I7VTB4 | 88.0 | 117 | 6 | 1 | 2 | 110 | 3 | 119 | 3.4e-48 | 200.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene27008 | 0.80766 | 4.53E-50 | -1.69E-46 |
| MS.gene049151 | MS.gene27008 | 0.812084 | 5.06E-51 | -1.69E-46 |
| MS.gene049159 | MS.gene27008 | 0.840672 | 7.55E-58 | -1.69E-46 |
| MS.gene049378 | MS.gene27008 | 0.837711 | 4.42E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene27008 | 0.801248 | 9.85E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene27008 | 0.819047 | 1.43E-52 | -1.69E-46 |
| MS.gene049647 | MS.gene27008 | 0.805559 | 1.26E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene27008 | 0.800476 | 1.42E-48 | -1.69E-46 |
| MS.gene04984 | MS.gene27008 | 0.80699 | 6.29E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene27008 | 0.838713 | 2.44E-57 | -1.69E-46 |
| MS.gene050227 | MS.gene27008 | 0.823139 | 1.63E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene27008 | 0.831243 | 1.86E-55 | -1.69E-46 |
| MS.gene050442 | MS.gene27008 | 0.849035 | 4.19E-60 | -1.69E-46 |
| MS.gene050605 | MS.gene27008 | 0.811651 | 6.29E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene27008 | -0.813434 | 2.57E-51 | -1.69E-46 |
| MS.gene050629 | MS.gene27008 | -0.808735 | 2.68E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene27008 | 0.857553 | 1.51E-62 | -1.69E-46 |
| MS.gene050724 | MS.gene27008 | 0.809713 | 1.65E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene27008 | 0.818246 | 2.17E-52 | -1.69E-46 |
| MS.gene050762 | MS.gene27008 | 0.850364 | 1.78E-60 | -1.69E-46 |
| MS.gene050763 | MS.gene27008 | 0.852191 | 5.43E-61 | -1.69E-46 |
| MS.gene050780 | MS.gene27008 | 0.815631 | 8.37E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene27008 | 0.82957 | 4.78E-55 | -1.69E-46 |
| MS.gene050820 | MS.gene27008 | 0.811458 | 6.93E-51 | -1.69E-46 |
| MS.gene050826 | MS.gene27008 | 0.856106 | 4.03E-62 | -1.69E-46 |
| MS.gene050953 | MS.gene27008 | 0.83087 | 2.30E-55 | -1.69E-46 |
| MS.gene050987 | MS.gene27008 | 0.807039 | 6.14E-50 | -1.69E-46 |
| MS.gene051180 | MS.gene27008 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene27008 | 0.813136 | 2.98E-51 | -1.69E-46 |
| MS.gene051210 | MS.gene27008 | 0.808548 | 2.93E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene27008 | 0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene27008 | 0.810901 | 9.15E-51 | -1.69E-46 |
| MS.gene051368 | MS.gene27008 | 0.818097 | 2.34E-52 | -1.69E-46 |
| MS.gene051452 | MS.gene27008 | 0.812236 | 4.69E-51 | -1.69E-46 |
| MS.gene051535 | MS.gene27008 | 0.809968 | 1.46E-50 | -1.69E-46 |
| MS.gene051800 | MS.gene27008 | 0.812278 | 4.59E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene27008 | 0.805226 | 1.48E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene27008 | 0.815612 | 8.46E-52 | -1.69E-46 |
| MS.gene052333 | MS.gene27008 | 0.805819 | 1.11E-49 | -1.69E-46 |
| MS.gene052426 | MS.gene27008 | 0.862 | 6.91E-64 | -1.69E-46 |
| MS.gene052665 | MS.gene27008 | 0.809004 | 2.34E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene27008 | 0.819444 | 1.16E-52 | -1.69E-46 |
| MS.gene052675 | MS.gene27008 | 0.818795 | 1.63E-52 | -1.69E-46 |
| MS.gene052677 | MS.gene27008 | 0.828157 | 1.05E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene27008 | 0.803826 | 2.90E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene27008 | 0.807209 | 5.65E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene27008 | 0.822818 | 1.93E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene27008 | 0.808407 | 3.14E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene27008 | 0.850072 | 2.15E-60 | -1.69E-46 |
| MS.gene053184 | MS.gene27008 | 0.812965 | 3.25E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene27008 | 0.811148 | 8.09E-51 | -1.69E-46 |
| MS.gene053494 | MS.gene27008 | 0.863327 | 2.69E-64 | -1.69E-46 |
| MS.gene053577 | MS.gene27008 | 0.810689 | 1.02E-50 | -1.69E-46 |
| MS.gene053584 | MS.gene27008 | 0.825909 | 3.62E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene27008 | 0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene27008 | 0.816738 | 4.74E-52 | -1.69E-46 |
| MS.gene05417 | MS.gene27008 | 0.800376 | 1.48E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene27008 | 0.842125 | 3.13E-58 | -1.69E-46 |
| MS.gene054379 | MS.gene27008 | 0.872922 | 2.19E-67 | -1.69E-46 |
| MS.gene054405 | MS.gene27008 | 0.822102 | 2.83E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene27008 | 0.818904 | 1.54E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene27008 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene054472 | MS.gene27008 | 0.812473 | 4.17E-51 | -1.69E-46 |
| MS.gene054542 | MS.gene27008 | 0.802953 | 4.39E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene27008 | 0.824048 | 9.97E-54 | -1.69E-46 |
| MS.gene05468 | MS.gene27008 | 0.810697 | 1.01E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene27008 | 0.855692 | 5.32E-62 | -1.69E-46 |
| MS.gene05510 | MS.gene27008 | 0.878833 | 2.04E-69 | -1.69E-46 |
| MS.gene055227 | MS.gene27008 | 0.825026 | 5.87E-54 | -1.69E-46 |
| MS.gene055356 | MS.gene27008 | 0.831203 | 1.91E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene27008 | 0.812261 | 4.63E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene27008 | 0.81112 | 8.21E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene27008 | 0.841896 | 3.60E-58 | -1.69E-46 |
| MS.gene055701 | MS.gene27008 | 0.890204 | 1.22E-73 | -1.69E-46 |
| MS.gene055818 | MS.gene27008 | 0.819938 | 8.93E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene27008 | 0.859018 | 5.53E-63 | -1.69E-46 |
| MS.gene05595 | MS.gene27008 | 0.807589 | 4.70E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene27008 | 0.842969 | 1.87E-58 | -1.69E-46 |
| MS.gene056173 | MS.gene27008 | 0.806531 | 7.86E-50 | -1.69E-46 |
| MS.gene056480 | MS.gene27008 | 0.828994 | 6.60E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene27008 | 0.84035 | 9.17E-58 | -1.69E-46 |
| MS.gene056760 | MS.gene27008 | 0.806795 | 6.92E-50 | -1.69E-46 |
| MS.gene056873 | MS.gene27008 | 0.815661 | 8.25E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene27008 | 0.80066 | 1.30E-48 | -1.69E-46 |
| MS.gene057194 | MS.gene27008 | 0.805738 | 1.15E-49 | -1.69E-46 |
| MS.gene057394 | MS.gene27008 | 0.836943 | 6.96E-57 | -1.69E-46 |
| MS.gene057395 | MS.gene27008 | 0.801409 | 9.13E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene27008 | 0.81006 | 1.39E-50 | -1.69E-46 |
| MS.gene05761 | MS.gene27008 | 0.83484 | 2.38E-56 | -1.69E-46 |
| MS.gene057750 | MS.gene27008 | 0.831934 | 1.26E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene27008 | 0.801592 | 8.37E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene27008 | 0.844103 | 9.30E-59 | -1.69E-46 |
| MS.gene057929 | MS.gene27008 | 0.801955 | 7.05E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene27008 | 0.800433 | 1.44E-48 | -1.69E-46 |
| MS.gene058146 | MS.gene27008 | 0.859376 | 4.32E-63 | -1.69E-46 |
| MS.gene058399 | MS.gene27008 | 0.812165 | 4.86E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene27008 | 0.83702 | 6.65E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene27008 | 0.813701 | 2.24E-51 | -1.69E-46 |
| MS.gene059124 | MS.gene27008 | 0.823222 | 1.56E-53 | -1.69E-46 |
| MS.gene059128 | MS.gene27008 | 0.863766 | 1.97E-64 | -1.69E-46 |
| MS.gene059161 | MS.gene27008 | 0.860615 | 1.83E-63 | -1.69E-46 |
| MS.gene059382 | MS.gene27008 | 0.808934 | 2.43E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene27008 | 0.82015 | 7.99E-53 | -1.69E-46 |
| MS.gene059713 | MS.gene27008 | 0.824262 | 8.88E-54 | -1.69E-46 |
| MS.gene059842 | MS.gene27008 | 0.804449 | 2.15E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene27008 | 0.839449 | 1.57E-57 | -1.69E-46 |
| MS.gene060167 | MS.gene27008 | 0.818034 | 2.42E-52 | -1.69E-46 |
| MS.gene060296 | MS.gene27008 | 0.805805 | 1.12E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene27008 | 0.825005 | 5.93E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene27008 | 0.851496 | 8.55E-61 | -1.69E-46 |
| MS.gene06052 | MS.gene27008 | 0.830979 | 2.16E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene27008 | 0.864806 | 9.33E-65 | -1.69E-46 |
| MS.gene060685 | MS.gene27008 | 0.860276 | 2.31E-63 | -1.69E-46 |
| MS.gene060802 | MS.gene27008 | 0.864533 | 1.14E-64 | -1.69E-46 |
| MS.gene060803 | MS.gene27008 | 0.846309 | 2.35E-59 | -1.69E-46 |
| MS.gene060940 | MS.gene27008 | 0.811566 | 6.57E-51 | -1.69E-46 |
| MS.gene060942 | MS.gene27008 | 0.81947 | 1.14E-52 | -1.69E-46 |
| MS.gene060943 | MS.gene27008 | 0.827899 | 1.21E-54 | -1.69E-46 |
| MS.gene060998 | MS.gene27008 | 0.883035 | 6.30E-71 | -1.69E-46 |
| MS.gene061002 | MS.gene27008 | 0.830629 | 2.64E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27008.t1 | MTR_1g023140 | 87.179 | 117 | 7 | 1 | 2 | 110 | 3 | 119 | 2.50e-67 | 198 |
| MS.gene27008.t1 | MTR_3g112410 | 75.455 | 110 | 19 | 1 | 2 | 103 | 3 | 112 | 9.77e-54 | 164 |
| MS.gene27008.t1 | MTR_5g021180 | 62.037 | 108 | 33 | 1 | 4 | 103 | 7 | 114 | 6.26e-44 | 139 |
| MS.gene27008.t1 | MTR_7g009070 | 49.020 | 102 | 44 | 1 | 11 | 104 | 32 | 133 | 6.39e-31 | 107 |
| MS.gene27008.t1 | MTR_8g107460 | 43.519 | 108 | 53 | 1 | 4 | 103 | 253 | 360 | 1.12e-25 | 98.6 |
| MS.gene27008.t1 | MTR_8g005320 | 37.736 | 106 | 58 | 1 | 3 | 100 | 24 | 129 | 7.60e-25 | 91.7 |
| MS.gene27008.t1 | MTR_3g112400 | 53.763 | 93 | 31 | 2 | 1 | 93 | 1 | 81 | 1.49e-24 | 89.4 |
| MS.gene27008.t1 | MTR_8g006685 | 37.736 | 106 | 58 | 1 | 3 | 100 | 93 | 198 | 2.41e-24 | 92.0 |
| MS.gene27008.t1 | MTR_8g006560 | 37.736 | 106 | 58 | 1 | 3 | 100 | 93 | 198 | 2.41e-24 | 92.0 |
| MS.gene27008.t1 | MTR_7g073680 | 35.714 | 112 | 64 | 1 | 3 | 106 | 22 | 133 | 1.11e-22 | 86.3 |
| MS.gene27008.t1 | MTR_5g037930 | 42.202 | 109 | 55 | 1 | 5 | 105 | 20 | 128 | 1.50e-21 | 82.8 |
| MS.gene27008.t1 | MTR_5g037950 | 42.453 | 106 | 53 | 1 | 7 | 104 | 21 | 126 | 1.41e-19 | 77.8 |
| MS.gene27008.t1 | MTR_5g037890 | 39.450 | 109 | 58 | 1 | 5 | 105 | 18 | 126 | 5.89e-18 | 73.6 |
| MS.gene27008.t1 | MTR_2g010750 | 33.913 | 115 | 68 | 1 | 4 | 110 | 8 | 122 | 5.68e-17 | 71.2 |
| MS.gene27008.t1 | MTR_4g094690 | 35.106 | 94 | 51 | 2 | 8 | 91 | 62 | 155 | 6.54e-16 | 69.7 |
| MS.gene27008.t1 | MTR_3g089065 | 35.780 | 109 | 57 | 3 | 8 | 106 | 70 | 175 | 2.01e-15 | 68.2 |
| MS.gene27008.t1 | MTR_1g098660 | 38.158 | 76 | 38 | 1 | 25 | 91 | 76 | 151 | 8.12e-15 | 66.6 |
| MS.gene27008.t1 | MTR_2g079420 | 39.474 | 76 | 37 | 1 | 25 | 91 | 84 | 159 | 1.17e-12 | 61.2 |
| MS.gene27008.t1 | MTR_2g082590 | 29.464 | 112 | 71 | 1 | 4 | 107 | 9 | 120 | 2.12e-12 | 59.3 |
| MS.gene27008.t1 | MTR_8g059015 | 35.227 | 88 | 47 | 2 | 25 | 102 | 87 | 174 | 4.84e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27008.t1 | AT3G51030 | 62.037 | 108 | 33 | 1 | 4 | 103 | 5 | 112 | 1.14e-46 | 146 |
| MS.gene27008.t1 | AT1G45145 | 55.856 | 111 | 41 | 1 | 1 | 103 | 1 | 111 | 3.30e-40 | 130 |
| MS.gene27008.t1 | AT5G42980 | 55.556 | 117 | 44 | 1 | 1 | 109 | 1 | 117 | 3.48e-40 | 129 |
| MS.gene27008.t1 | AT1G19730 | 53.846 | 117 | 45 | 1 | 2 | 109 | 3 | 119 | 4.36e-37 | 122 |
| MS.gene27008.t1 | AT5G39950 | 40.187 | 107 | 56 | 1 | 6 | 104 | 26 | 132 | 2.53e-24 | 90.1 |
| MS.gene27008.t1 | AT3G17880 | 37.037 | 108 | 60 | 1 | 4 | 103 | 262 | 369 | 2.61e-24 | 95.1 |
| MS.gene27008.t1 | AT3G17880 | 37.037 | 108 | 60 | 1 | 4 | 103 | 269 | 376 | 2.85e-24 | 95.1 |
| MS.gene27008.t1 | AT3G56420 | 38.462 | 104 | 56 | 1 | 2 | 97 | 37 | 140 | 1.50e-23 | 89.0 |
| MS.gene27008.t1 | AT3G56420 | 38.462 | 104 | 56 | 1 | 2 | 97 | 37 | 140 | 1.50e-23 | 89.0 |
| MS.gene27008.t1 | AT3G56420 | 38.462 | 104 | 56 | 1 | 2 | 97 | 37 | 140 | 1.50e-23 | 89.0 |
| MS.gene27008.t1 | AT3G08710 | 34.545 | 110 | 64 | 1 | 5 | 106 | 23 | 132 | 2.78e-22 | 85.1 |
| MS.gene27008.t1 | AT3G08710 | 34.545 | 110 | 64 | 1 | 5 | 106 | 23 | 132 | 2.78e-22 | 85.1 |
| MS.gene27008.t1 | AT3G08710 | 34.545 | 110 | 64 | 1 | 5 | 106 | 23 | 132 | 2.78e-22 | 85.1 |
| MS.gene27008.t1 | AT1G59730 | 36.607 | 112 | 63 | 1 | 1 | 104 | 17 | 128 | 3.13e-21 | 82.0 |
| MS.gene27008.t1 | AT1G69880 | 36.190 | 105 | 59 | 1 | 7 | 103 | 39 | 143 | 1.62e-20 | 80.9 |
| MS.gene27008.t1 | AT2G40790 | 38.235 | 102 | 55 | 1 | 4 | 97 | 39 | 140 | 2.41e-19 | 78.2 |
| MS.gene27008.t1 | AT2G40790 | 38.235 | 102 | 55 | 1 | 4 | 97 | 39 | 140 | 2.41e-19 | 78.2 |
| MS.gene27008.t1 | AT1G11530 | 34.653 | 101 | 58 | 1 | 5 | 97 | 2 | 102 | 1.26e-14 | 65.1 |
| MS.gene27008.t1 | AT1G76760 | 36.842 | 76 | 39 | 1 | 25 | 91 | 79 | 154 | 2.63e-14 | 65.5 |
| MS.gene27008.t1 | AT2G35010 | 32.110 | 109 | 64 | 1 | 2 | 100 | 81 | 189 | 2.77e-14 | 65.9 |
| MS.gene27008.t1 | AT2G35010 | 32.110 | 109 | 64 | 1 | 2 | 100 | 81 | 189 | 2.77e-14 | 65.9 |
| MS.gene27008.t1 | AT1G43560 | 35.556 | 90 | 44 | 2 | 25 | 100 | 74 | 163 | 8.42e-14 | 63.9 |
| MS.gene27008.t1 | AT5G16400 | 35.366 | 82 | 44 | 1 | 28 | 100 | 98 | 179 | 6.53e-11 | 56.6 |
Find 27 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGCAATCTCAGCTAGAAT+TGG | 0.355652 | 1.3:-2221286 | None:intergenic |
| AGAAGGTAAGGAAGTGGATA+AGG | 0.412033 | 1.3:+2233820 | MS.gene27008:CDS |
| CTTATTCTTGAAAGAAGGTA+AGG | 0.442943 | 1.3:+2233808 | MS.gene27008:CDS |
| ACCTTCTTATTCTTGAAAGA+AGG | 0.451509 | 1.3:+2233803 | MS.gene27008:CDS |
| AAATGCAATCACCAAGCATA+AGG | 0.465102 | 1.3:+2233871 | MS.gene27008:CDS |
| TAAGGTAGTTGGTGCTAGGA+AGG | 0.479587 | 1.3:+2233838 | MS.gene27008:CDS |
| TGGATAAGGTAGTTGGTGCT+AGG | 0.488936 | 1.3:+2233834 | MS.gene27008:CDS |
| AAGGAAGTGGATAAGGTAGT+TGG | 0.491604 | 1.3:+2233827 | MS.gene27008:CDS |
| AACAGTTGCATCCTTATGCT+TGG | 0.492616 | 1.3:-2233882 | None:intergenic |
| ACCTTCTTTCAAGAATAAGA+AGG | 0.495256 | 1.3:-2233804 | None:intergenic |
| TTCAGAGTGTTGCTGAGGAA+TGG | 0.506641 | 1.3:+2233762 | MS.gene27008:intron |
| CTAGAATTGGAGCAATAAAA+CGG | 0.524722 | 1.3:-2221273 | None:intergenic |
| CTTGAAAGAAGGTAAGGAAG+TGG | 0.537499 | 1.3:+2233814 | MS.gene27008:CDS |
| TGGTGCTAGGAAGGAAGAGC+TGG | 0.539272 | 1.3:+2233847 | MS.gene27008:CDS |
| TGCTAAGAAGACTCCTGTGT+TGG | 0.542035 | 1.3:+2221303 | MS.gene27008:CDS |
| TCTTTCAAGAATAAGAAGGT+TGG | 0.555397 | 1.3:-2233800 | None:intergenic |
| CTCCTCCTTCCACTGTTCAA+CGG | 0.563671 | 1.3:-2219660 | None:intergenic |
| CATTGAATAATAGATTGTGG+TGG | 0.597012 | 1.3:+2221222 | MS.gene27008:intron |
| GCAGCTGAAGGACAGGTGAT+CGG | 0.598988 | 1.3:+2219629 | MS.gene27008:CDS |
| GTGGAAGGAGGAGATCCAGA+AGG | 0.606920 | 1.3:+2219670 | MS.gene27008:CDS |
| TGGAAGGAGGAGATCCAGAA+GGG | 0.610530 | 1.3:+2219671 | MS.gene27008:CDS |
| TGCTGAGGAATGGTCTGTTG+AGG | 0.618156 | 1.3:+2233772 | MS.gene27008:CDS |
| TCATACCGTTGAACAGTGGA+AGG | 0.620633 | 1.3:+2219655 | MS.gene27008:CDS |
| ATTGGAGCAATAAAACGGCA+TGG | 0.627618 | 1.3:-2221268 | None:intergenic |
| CCTATTTCAGAGTGTTGCTG+AGG | 0.630806 | 1.3:+2233757 | MS.gene27008:intron |
| TACCGTTGAACAGTGGAAGG+AGG | 0.648899 | 1.3:+2219658 | MS.gene27008:CDS |
| GTGTTCATACCGTTGAACAG+TGG | 0.666984 | 1.3:+2219651 | MS.gene27008:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 2219626 | 2233916 | 2219626 | ID=MS.gene27008 |
| chr1.3 | mRNA | 2219626 | 2233916 | 2219626 | ID=MS.gene27008.t1;Parent=MS.gene27008 |
| chr1.3 | exon | 2219626 | 2219712 | 2219626 | ID=MS.gene27008.t1.exon1;Parent=MS.gene27008.t1 |
| chr1.3 | CDS | 2219626 | 2219712 | 2219626 | ID=cds.MS.gene27008.t1;Parent=MS.gene27008.t1 |
| chr1.3 | exon | 2221235 | 2221333 | 2221235 | ID=MS.gene27008.t1.exon2;Parent=MS.gene27008.t1 |
| chr1.3 | CDS | 2221235 | 2221333 | 2221235 | ID=cds.MS.gene27008.t1;Parent=MS.gene27008.t1 |
| chr1.3 | exon | 2233767 | 2233916 | 2233767 | ID=MS.gene27008.t1.exon3;Parent=MS.gene27008.t1 |
| chr1.3 | CDS | 2233767 | 2233916 | 2233767 | ID=cds.MS.gene27008.t1;Parent=MS.gene27008.t1 |
| Gene Sequence |
| Protein sequence |
