AlfalfaGEDB | MS.gene21911

M. sativa cultivar XinJiangDaYe / MS.gene21911

KEGG: K18167
GO: -
PFAM: PF05347.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21911.t1	XP_003610904.1	92.5	93	7	0	1	93	1	93	7.50E-39	169.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21911.t1	A6ZYX9	39.0	77	46	1	7	83	3	78	1.1e-06	53.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21911.t1	G7K7V7	92.5	93	7	0	1	93	1	93	5.4e-39	169.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene21911	0.837913	3.93E-57	-1.69E-46
MS.gene049378	MS.gene21911	0.836172	1.09E-56	-1.69E-46
MS.gene049424	MS.gene21911	0.800661	1.30E-48	-1.69E-46
MS.gene049435	MS.gene21911	0.815175	1.06E-51	-1.69E-46
MS.gene049669	MS.gene21911	0.801454	8.93E-49	-1.69E-46
MS.gene049756	MS.gene21911	0.823136	1.63E-53	-1.69E-46
MS.gene04984	MS.gene21911	0.820737	5.86E-53	-1.69E-46
MS.gene050226	MS.gene21911	0.823936	1.06E-53	-1.69E-46
MS.gene050227	MS.gene21911	0.830128	3.50E-55	-1.69E-46
MS.gene050228	MS.gene21911	0.826522	2.59E-54	-1.69E-46
MS.gene050438	MS.gene21911	0.803066	4.16E-49	-1.69E-46
MS.gene050442	MS.gene21911	0.84583	3.18E-59	-1.69E-46
MS.gene050586	MS.gene21911	0.802548	5.33E-49	-1.69E-46
MS.gene050605	MS.gene21911	0.821333	4.27E-53	-1.69E-46
MS.gene050623	MS.gene21911	-0.823058	1.70E-53	-1.69E-46
MS.gene050680	MS.gene21911	0.805644	1.21E-49	-1.69E-46
MS.gene050719	MS.gene21911	0.854469	1.21E-61	-1.69E-46
MS.gene050721	MS.gene21911	0.852186	5.44E-61	-1.69E-46
MS.gene050724	MS.gene21911	0.862029	6.77E-64	-1.69E-46
MS.gene050750	MS.gene21911	0.815405	9.40E-52	-1.69E-46
MS.gene050759	MS.gene21911	0.800054	1.73E-48	-1.69E-46
MS.gene050762	MS.gene21911	0.803697	3.08E-49	-1.69E-46
MS.gene050763	MS.gene21911	0.804622	1.98E-49	-1.69E-46
MS.gene050780	MS.gene21911	0.824193	9.21E-54	-1.69E-46
MS.gene050815	MS.gene21911	0.835048	2.10E-56	-1.69E-46
MS.gene050844	MS.gene21911	0.809333	1.99E-50	-1.69E-46
MS.gene050953	MS.gene21911	0.834012	3.83E-56	-1.69E-46
MS.gene050987	MS.gene21911	0.800911	1.15E-48	-1.69E-46
MS.gene051243	MS.gene21911	0.838793	2.33E-57	-1.69E-46
MS.gene051328	MS.gene21911	0.800162	1.64E-48	-1.69E-46
MS.gene051349	MS.gene21911	0.824979	6.02E-54	-1.69E-46
MS.gene051452	MS.gene21911	0.829465	5.07E-55	-1.69E-46
MS.gene051535	MS.gene21911	0.821382	4.16E-53	-1.69E-46
MS.gene051757	MS.gene21911	0.825453	4.65E-54	-1.69E-46
MS.gene052012	MS.gene21911	0.83293	7.14E-56	-1.69E-46
MS.gene052176	MS.gene21911	0.820159	7.95E-53	-1.69E-46
MS.gene052376	MS.gene21911	0.811739	6.02E-51	-1.69E-46
MS.gene052426	MS.gene21911	0.880287	6.21E-70	-1.69E-46
MS.gene052773	MS.gene21911	0.802923	4.46E-49	-1.69E-46
MS.gene052863	MS.gene21911	0.810942	8.97E-51	-1.69E-46
MS.gene05288	MS.gene21911	0.819715	1.00E-52	-1.69E-46
MS.gene052896	MS.gene21911	0.818999	1.46E-52	-1.69E-46
MS.gene05289	MS.gene21911	0.810874	9.28E-51	-1.69E-46
MS.gene053008	MS.gene21911	0.816287	5.98E-52	-1.69E-46
MS.gene053074	MS.gene21911	0.80956	1.78E-50	-1.69E-46
MS.gene053166	MS.gene21911	0.813622	2.33E-51	-1.69E-46
MS.gene053494	MS.gene21911	0.803186	3.93E-49	-1.69E-46
MS.gene05415	MS.gene21911	0.806113	9.63E-50	-1.69E-46
MS.gene054379	MS.gene21911	0.800638	1.31E-48	-1.69E-46
MS.gene054405	MS.gene21911	0.81595	7.11E-52	-1.69E-46
MS.gene054467	MS.gene21911	0.807497	4.91E-50	-1.69E-46
MS.gene054472	MS.gene21911	0.800029	1.75E-48	-1.69E-46
MS.gene054542	MS.gene21911	0.803259	3.80E-49	-1.69E-46
MS.gene054559	MS.gene21911	0.800158	1.64E-48	-1.69E-46
MS.gene054657	MS.gene21911	0.814656	1.38E-51	-1.69E-46
MS.gene054658	MS.gene21911	0.828163	1.05E-54	-1.69E-46
MS.gene054930	MS.gene21911	0.818292	2.12E-52	-1.69E-46
MS.gene05510	MS.gene21911	0.860814	1.59E-63	-1.69E-46
MS.gene05517	MS.gene21911	0.801768	7.71E-49	-1.69E-46
MS.gene055623	MS.gene21911	0.85494	8.81E-62	-1.69E-46
MS.gene055701	MS.gene21911	0.829622	4.64E-55	-1.69E-46
MS.gene055818	MS.gene21911	0.817315	3.52E-52	-1.69E-46
MS.gene055926	MS.gene21911	0.81161	6.42E-51	-1.69E-46
MS.gene05604	MS.gene21911	0.816091	6.61E-52	-1.69E-46
MS.gene056480	MS.gene21911	0.80244	5.60E-49	-1.69E-46
MS.gene056506	MS.gene21911	0.826565	2.53E-54	-1.69E-46
MS.gene056760	MS.gene21911	0.832328	1.01E-55	-1.69E-46
MS.gene056873	MS.gene21911	0.809672	1.69E-50	-1.69E-46
MS.gene057140	MS.gene21911	0.829983	3.79E-55	-1.69E-46
MS.gene057200	MS.gene21911	0.802301	5.99E-49	-1.69E-46
MS.gene057395	MS.gene21911	0.822617	2.15E-53	-1.69E-46
MS.gene05761	MS.gene21911	0.804738	1.87E-49	-1.69E-46
MS.gene057693	MS.gene21911	0.837387	5.36E-57	-1.69E-46
MS.gene057750	MS.gene21911	0.822697	2.06E-53	-1.69E-46
MS.gene057869	MS.gene21911	0.8133	2.74E-51	-1.69E-46
MS.gene058146	MS.gene21911	0.881737	1.87E-70	-1.69E-46
MS.gene058168	MS.gene21911	0.80004	1.74E-48	-1.69E-46
MS.gene058399	MS.gene21911	0.802959	4.38E-49	-1.69E-46
MS.gene058435	MS.gene21911	0.801911	7.20E-49	-1.69E-46
MS.gene058548	MS.gene21911	0.814462	1.52E-51	-1.69E-46
MS.gene058595	MS.gene21911	0.825927	3.59E-54	-1.69E-46
MS.gene058906	MS.gene21911	0.811609	6.43E-51	-1.69E-46
MS.gene058979	MS.gene21911	0.810386	1.18E-50	-1.69E-46
MS.gene059124	MS.gene21911	0.805577	1.25E-49	-1.69E-46
MS.gene059128	MS.gene21911	0.803927	2.76E-49	-1.69E-46
MS.gene059249	MS.gene21911	0.817974	2.50E-52	-1.69E-46
MS.gene05979	MS.gene21911	0.811947	5.43E-51	-1.69E-46
MS.gene059830	MS.gene21911	0.807125	5.89E-50	-1.69E-46
MS.gene059842	MS.gene21911	0.810246	1.27E-50	-1.69E-46
MS.gene060011	MS.gene21911	0.80389	2.81E-49	-1.69E-46
MS.gene060084	MS.gene21911	0.803386	3.57E-49	-1.69E-46
MS.gene060310	MS.gene21911	0.815191	1.05E-51	-1.69E-46
MS.gene060322	MS.gene21911	0.860086	2.64E-63	-1.69E-46
MS.gene060572	MS.gene21911	0.815399	9.43E-52	-1.69E-46
MS.gene060677	MS.gene21911	0.82955	4.84E-55	-1.69E-46
MS.gene060683	MS.gene21911	0.815707	8.05E-52	-1.69E-46
MS.gene060685	MS.gene21911	0.817221	3.69E-52	-1.69E-46
MS.gene060802	MS.gene21911	0.838921	2.16E-57	-1.69E-46
MS.gene060803	MS.gene21911	0.847456	1.14E-59	-1.69E-46
MS.gene060998	MS.gene21911	0.862039	6.72E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21911.t1	MTR_5g008340	92.473	93	7	0	1	93	1	93	1.76e-59	177

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21911.t1	AT2G39725	67.391	92	27	1	1	92	1	89	1.34e-38	124
MS.gene21911.t1	AT2G39725	67.391	92	27	1	1	92	1	89	1.34e-38	124

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAGAGTGCAAAGGCTTTC+TGG	0.170483	5.1:+2396682	MS.gene21911:CDS
GCGGCGTTCTTCGTCGTGTT+TGG	0.337693	5.1:-2396758	None:intergenic
CTTAACAACCCTGGTACCAC+TGG	0.425728	5.1:+2396895	MS.gene21911:CDS
GAAGATAACCCAGTGGTACC+AGG	0.475476	5.1:-2396904	None:intergenic
TCCAGAAAGCCTTTGCACTC+TGG	0.484965	5.1:-2396683	None:intergenic
ATTGAATATTTACTTCGTCG+TGG	0.613668	5.1:+2396853	MS.gene21911:CDS
AAGATAACCCAGTGGTACCA+GGG	0.621203	5.1:-2396903	None:intergenic
ATACTAAAGATTCTATGTTG+CGG	0.622444	5.1:-2396777	None:intergenic
TTAACAACCCTGGTACCACT+GGG	0.624488	5.1:+2396896	MS.gene21911:CDS
CAAGTGCTTAGCTTATACAG+AGG	0.632036	5.1:+2396715	MS.gene21911:CDS
TGGGAGGAACCAGAGTGCAA+AGG	0.649416	5.1:+2396674	MS.gene21911:CDS
CTTGATCAGCTTAACAACCC+TGG	0.668759	5.1:+2396886	MS.gene21911:CDS
TTGCAATGAAGATAACCCAG+TGG	0.758644	5.1:-2396911	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GATCAATTTCTTTTGAATTA+CGG	-	chr5.1:2396816-2396835	None:intergenic	20.0%
!	ATACTAAAGATTCTATGTTG+CGG	-	chr5.1:2396780-2396799	None:intergenic	25.0%
	ATTGAATATTTACTTCGTCG+TGG	+	chr5.1:2396853-2396872	MS.gene21911:CDS	30.0%
	CAAGTGCTTAGCTTATACAG+AGG	+	chr5.1:2396715-2396734	MS.gene21911:CDS	40.0%
	TTGCAATGAAGATAACCCAG+TGG	-	chr5.1:2396914-2396933	None:intergenic	40.0%
	AAGATAACCCAGTGGTACCA+GGG	-	chr5.1:2396906-2396925	None:intergenic	45.0%
	CTTGATCAGCTTAACAACCC+TGG	+	chr5.1:2396886-2396905	MS.gene21911:CDS	45.0%
	TTAACAACCCTGGTACCACT+GGG	+	chr5.1:2396896-2396915	MS.gene21911:CDS	45.0%
	ACCAGAGTGCAAAGGCTTTC+TGG	+	chr5.1:2396682-2396701	MS.gene21911:CDS	50.0%
	CTTAACAACCCTGGTACCAC+TGG	+	chr5.1:2396895-2396914	MS.gene21911:CDS	50.0%
	GAAGATAACCCAGTGGTACC+AGG	-	chr5.1:2396907-2396926	None:intergenic	50.0%
	TCCAGAAAGCCTTTGCACTC+TGG	-	chr5.1:2396686-2396705	None:intergenic	50.0%
	TGGGAGGAACCAGAGTGCAA+AGG	+	chr5.1:2396674-2396693	MS.gene21911:CDS	55.0%
	GCGGCGTTCTTCGTCGTGTT+TGG	-	chr5.1:2396761-2396780	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	2396673	2396954	2396673	ID=MS.gene21911
chr5.1	mRNA	2396673	2396954	2396673	ID=MS.gene21911.t1;Parent=MS.gene21911
chr5.1	exon	2396673	2396954	2396673	ID=MS.gene21911.t1.exon1;Parent=MS.gene21911.t1
chr5.1	CDS	2396673	2396954	2396673	ID=cds.MS.gene21911.t1;Parent=MS.gene21911.t1

Gene Sequence	>MS.gene21911 ATGGGAGGAACCAGAGTGCAAAGGCTTTCTGGAATGCAAAAGCAAGTGCTTAGCTTATACAGAGGATTTCTACGCGCAGCACGTTCCAAACACGACGAAGAACGCCGCAACATAGAATCTTTAGTATCTCAAGAATTTCGCCGTAATTCAAAAGAAATTGATCGAAAGAATTTTCAATACATTGAATATTTACTTCGTCGTGGTCATAAACAGCTTGATCAGCTTAACAACCCTGGTACCACTGGGTTATCTTCATTGCAACTTCATTTATCTAAAGATTAA
Protein sequence	>MS.gene21911.t1 MGGTRVQRLSGMQKQVLSLYRGFLRAARSKHDEERRNIESLVSQEFRRNSKEIDRKNFQYIEYLLRRGHKQLDQLNNPGTTGLSSLQLHLSKD