AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48646


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48646.t1 MTR_4g032805 96.371 248 8 1 1 248 23 269 0.0 498
MS.gene48646.t1 MTR_2g097690 50.000 62 29 1 109 170 77 136 3.52e-12 63.9
MS.gene48646.t1 MTR_5g022180 36.264 91 55 2 87 176 53 141 7.03e-12 62.4
MS.gene48646.t1 MTR_2g097690 50.000 64 30 1 107 170 75 136 1.34e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48646.t1 AT4G09350 59.389 229 82 3 20 247 28 246 8.25e-96 281
MS.gene48646.t1 AT5G59610 49.231 65 31 1 105 169 71 133 2.68e-11 62.4
MS.gene48646.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48646.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48646.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2
MS.gene48646.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2

Find 39 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACCCAAAAGCTTCTT+AGG 0.196506 4.1:-67979362 None:intergenic
CTACTATTCAATCCAATCTT+AGG 0.223430 4.1:+67979128 MS.gene48646:CDS
GAATATTTGTGCAGATTCTT+AGG 0.256272 4.1:+67980089 MS.gene48646:intron
TCTTATTCTTCTTATTGTTT+TGG 0.269689 4.1:-67979156 None:intergenic
GGAGAAACCTAAGAAGCTTT+TGG 0.298843 4.1:+67979355 MS.gene48646:CDS
TGCATTTGTTTGTTGCTGTT+TGG 0.332246 4.1:-67979330 None:intergenic
ATTGCTTCTACATCAGCTTC+TGG 0.336747 4.1:-67980119 None:intergenic
GCAAGTTCATGCTTCTACTA+AGG 0.365942 4.1:+67979220 MS.gene48646:CDS
GAAACTGAAAATAAAATTAG+AGG 0.366458 4.1:+67980322 MS.gene48646:CDS
AGAGGGAGTGAAGTTGTGTC+TGG 0.393350 4.1:-67980179 None:intergenic
AAGCAATCAAAGTAGCATTC+AGG 0.395851 4.1:+67980135 MS.gene48646:CDS
AAAGTAGAAGATTCTATGAT+TGG 0.404469 4.1:+67980264 MS.gene48646:CDS
TTCTTCAAGGCTACCTCATA+AGG 0.409509 4.1:-67980347 None:intergenic
CAAACTGTAGTGAGAGTTTG+AGG 0.431351 4.1:-67979198 None:intergenic
GGATTGAATAGTAGAATTTG+TGG 0.442017 4.1:-67979119 None:intergenic
AACAGGTTCATAATTCTTCA+AGG 0.442631 4.1:-67980360 None:intergenic
CCAAGACGATCAACCATGTC+TGG 0.451377 4.1:-67980383 None:intergenic
CGATCAACCATGTCTGGAAC+AGG 0.460674 4.1:-67980377 None:intergenic
GATGATGAAGGTTGGATTGG+AGG 0.480496 4.1:+67979302 MS.gene48646:CDS
AATAGTAGAATTTGTGGAGA+AGG 0.498829 4.1:-67979113 None:intergenic
AAAATTAGAGGATCCTTATG+AGG 0.498846 4.1:+67980334 MS.gene48646:CDS
TACATGAGTGATACAGCATA+TGG 0.500284 4.1:-67980477 None:intergenic
AATGATGATGAAGGTTGGAT+TGG 0.510553 4.1:+67979299 MS.gene48646:CDS
TGGATTCTTGTGTCAACTCC+AGG 0.515932 4.1:-67979269 None:intergenic
GGGAGAATGATGATGAAGGT+TGG 0.524754 4.1:+67979294 MS.gene48646:CDS
CTTCATCATCATTCTCCCAA+TGG 0.525735 4.1:-67979289 None:intergenic
GACTTGCAACTTCTTGAGCA+AGG 0.527830 4.1:-67980289 None:intergenic
GACATGGTTGATCGTCTTGG+TGG 0.530101 4.1:+67980386 MS.gene48646:CDS
CCAGACATGGTTGATCGTCT+TGG 0.532935 4.1:+67980383 MS.gene48646:CDS
AGTTGACACAAGAATCCATT+GGG 0.545872 4.1:+67979274 MS.gene48646:CDS
TTATGAACCTGTTCCAGACA+TGG 0.570153 4.1:+67980370 MS.gene48646:CDS
ACTTGCAACTTCTTGAGCAA+GGG 0.574767 4.1:-67980288 None:intergenic
AGCATTCAGGAAACTTTCAA+AGG 0.585269 4.1:+67980148 MS.gene48646:CDS
ACATGGTTGATCGTCTTGGT+GGG 0.606623 4.1:+67980387 MS.gene48646:CDS
CATTGGGAGAATGATGATGA+AGG 0.623650 4.1:+67979290 MS.gene48646:CDS
GAGTTGACACAAGAATCCAT+TGG 0.644255 4.1:+67979273 MS.gene48646:CDS
ACAGCAACAAACAAATGCAG+AGG 0.656916 4.1:+67979334 MS.gene48646:CDS
GCTTCTACTAAGGACACTCA+AGG 0.674014 4.1:+67979230 MS.gene48646:CDS
ATTCTTGTGTCAACTCCAGG+TGG 0.739348 4.1:-67979266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGAAGAATAAGATTTTTT+AGG + chr4.1:67979165-67979184 MS.gene48646:CDS 15.0%
!!! TAGTTTAAAATTGAATTTCA+AGG - chr4.1:67979519-67979538 None:intergenic 15.0%
!! GAAACTGAAAATAAAATTAG+AGG + chr4.1:67980322-67980341 MS.gene48646:CDS 20.0%
!!! ATTAAATTCTGTTTTTCTTC+AGG + chr4.1:67979937-67979956 MS.gene48646:intron 20.0%
!!! CGTTTTAAAAAAAGAAAAAC+TGG + chr4.1:67979980-67979999 MS.gene48646:intron 20.0%
!!! TAATTTCATGAATTTTTCTG+AGG - chr4.1:67980213-67980232 None:intergenic 20.0%
!!! TCTTATTCTTCTTATTGTTT+TGG - chr4.1:67979159-67979178 None:intergenic 20.0%
!!! TTTATACTTTTTGTAGGAAA+CGG - chr4.1:67979443-67979462 None:intergenic 20.0%
! AAAGTAGAAGATTCTATGAT+TGG + chr4.1:67980264-67980283 MS.gene48646:CDS 25.0%
! AGACTAAAACAACTATTAGT+CGG + chr4.1:67979772-67979791 MS.gene48646:intron 25.0%
!! TTATTTTCAGTTTCTCTTGT+TGG - chr4.1:67980316-67980335 None:intergenic 25.0%
!!! TGGTGTTTTATACTTTTTGT+AGG - chr4.1:67979449-67979468 None:intergenic 25.0%
AAAATTAGAGGATCCTTATG+AGG + chr4.1:67980334-67980353 MS.gene48646:CDS 30.0%
AACAGGTTCATAATTCTTCA+AGG - chr4.1:67980363-67980382 None:intergenic 30.0%
AATAGTAGAATTTGTGGAGA+AGG - chr4.1:67979116-67979135 None:intergenic 30.0%
AGTTCGTATAAGTATGTGTT+TGG + chr4.1:67979540-67979559 MS.gene48646:intron 30.0%
ATGTCAGAGTTTGATCAAAA+AGG - chr4.1:67979807-67979826 None:intergenic 30.0%
CTACTATTCAATCCAATCTT+AGG + chr4.1:67979128-67979147 MS.gene48646:CDS 30.0%
GAATATTTGTGCAGATTCTT+AGG + chr4.1:67980089-67980108 MS.gene48646:intron 30.0%
GGATTGAATAGTAGAATTTG+TGG - chr4.1:67979122-67979141 None:intergenic 30.0%
GGCAAAATTCAGTTTACAAT+CGG - chr4.1:67979498-67979517 None:intergenic 30.0%
TCAGCTAATGTTGAATAGTT+TGG + chr4.1:67979720-67979739 MS.gene48646:intron 30.0%
TGACAAGAGTTACTGAATAA+TGG + chr4.1:67980067-67980086 MS.gene48646:intron 30.0%
TGATCAAACTCTGACATAAT+CGG + chr4.1:67979810-67979829 MS.gene48646:intron 30.0%
TTTCTCATGATACAAATCTC+TGG - chr4.1:67980032-67980051 None:intergenic 30.0%
! AACCACTTCGATTTTCTATT+AGG + chr4.1:67979471-67979490 MS.gene48646:intron 30.0%
! ACATAATCGGTACATGTTTT+GGG + chr4.1:67979823-67979842 MS.gene48646:intron 30.0%
! AGTTATACTTAGTGCTCTTT+TGG - chr4.1:67979692-67979711 None:intergenic 30.0%
! CGGTGGTTTTTATAAAATCA+CGG - chr4.1:67979663-67979682 None:intergenic 30.0%
! CGTGATTTTATAAAAACCAC+CGG + chr4.1:67979661-67979680 MS.gene48646:intron 30.0%
! TAGAAAATCGAAGTGGTTTT+TGG - chr4.1:67979469-67979488 None:intergenic 30.0%
AAGCAATCAAAGTAGCATTC+AGG + chr4.1:67980135-67980154 MS.gene48646:CDS 35.0%
AGCATTCAGGAAACTTTCAA+AGG + chr4.1:67980148-67980167 MS.gene48646:CDS 35.0%
AGTTGACACAAGAATCCATT+GGG + chr4.1:67979274-67979293 MS.gene48646:CDS 35.0%
ATCTCTGGTAACTAGTAAGT+TGG - chr4.1:67980017-67980036 None:intergenic 35.0%
GACCTAATAGAAAATCGAAG+TGG - chr4.1:67979476-67979495 None:intergenic 35.0%
TACATGAGTGATACAGCATA+TGG - chr4.1:67980480-67980499 None:intergenic 35.0%
TACGACTTGAGTTCAATTGT+TGG + chr4.1:67979744-67979763 MS.gene48646:intron 35.0%
! AATGATGATGAAGGTTGGAT+TGG + chr4.1:67979299-67979318 MS.gene48646:CDS 35.0%
! GACATAATCGGTACATGTTT+TGG + chr4.1:67979822-67979841 MS.gene48646:intron 35.0%
! GAGAAACCTAAGAAGCTTTT+GGG + chr4.1:67979356-67979375 MS.gene48646:CDS 35.0%
! TGCATTTGTTTGTTGCTGTT+TGG - chr4.1:67979333-67979352 None:intergenic 35.0%
!! AAAGCTTGCTTTTGTAGCTT+CGG - chr4.1:67979620-67979639 None:intergenic 35.0%
!!! GCTGATTTGCTCAATTTTCA+AGG + chr4.1:67979389-67979408 MS.gene48646:CDS 35.0%
!!! GTTTTGGTGTTTCCTAAGAT+TGG - chr4.1:67979143-67979162 None:intergenic 35.0%
!!! TGTTTGGACTAGCTTTTACA+AGG + chr4.1:67979556-67979575 MS.gene48646:intron 35.0%
!!! TTTCTGAGGCTGTTTTTAGA+GGG - chr4.1:67980199-67980218 None:intergenic 35.0%
!!! TTTTCTGAGGCTGTTTTTAG+AGG - chr4.1:67980200-67980219 None:intergenic 35.0%
ACAGCAACAAACAAATGCAG+AGG + chr4.1:67979334-67979353 MS.gene48646:CDS 40.0%
ACTTGCAACTTCTTGAGCAA+GGG - chr4.1:67980291-67980310 None:intergenic 40.0%
ATTGCTTCTACATCAGCTTC+TGG - chr4.1:67980122-67980141 None:intergenic 40.0%
CAAACTGTAGTGAGAGTTTG+AGG - chr4.1:67979201-67979220 None:intergenic 40.0%
CTTCATCATCATTCTCCCAA+TGG - chr4.1:67979292-67979311 None:intergenic 40.0%
GAGTTGACACAAGAATCCAT+TGG + chr4.1:67979273-67979292 MS.gene48646:CDS 40.0%
GCAAGTTCATGCTTCTACTA+AGG + chr4.1:67979220-67979239 MS.gene48646:CDS 40.0%
TCTTCACCCAAAAGCTTCTT+AGG - chr4.1:67979365-67979384 None:intergenic 40.0%
TTATGAACCTGTTCCAGACA+TGG + chr4.1:67980370-67980389 MS.gene48646:CDS 40.0%
TTCTTCAAGGCTACCTCATA+AGG - chr4.1:67980350-67980369 None:intergenic 40.0%
! CATTGGGAGAATGATGATGA+AGG + chr4.1:67979290-67979309 MS.gene48646:CDS 40.0%
! GGAGAAACCTAAGAAGCTTT+TGG + chr4.1:67979355-67979374 MS.gene48646:CDS 40.0%
!! AAGCTTGCTTTTGTAGCTTC+GGG - chr4.1:67979619-67979638 None:intergenic 40.0%
!! TTTTCACGTATAGCTTGCCT+CGG - chr4.1:67979595-67979614 None:intergenic 40.0%
!!! TAGTGCTCTTTTGGTTTAGC+CGG - chr4.1:67979683-67979702 None:intergenic 40.0%
ACATGGTTGATCGTCTTGGT+GGG + chr4.1:67980387-67980406 MS.gene48646:CDS 45.0%
ATTCTTGTGTCAACTCCAGG+TGG - chr4.1:67979269-67979288 None:intergenic 45.0%
GACTTGCAACTTCTTGAGCA+AGG - chr4.1:67980292-67980311 None:intergenic 45.0%
GCTTCTACTAAGGACACTCA+AGG + chr4.1:67979230-67979249 MS.gene48646:CDS 45.0%
TGGATTCTTGTGTCAACTCC+AGG - chr4.1:67979272-67979291 None:intergenic 45.0%
! GATGATGAAGGTTGGATTGG+AGG + chr4.1:67979302-67979321 MS.gene48646:CDS 45.0%
! GGGAGAATGATGATGAAGGT+TGG + chr4.1:67979294-67979313 MS.gene48646:CDS 45.0%
! TTTCACGTATAGCTTGCCTC+GGG - chr4.1:67979594-67979613 None:intergenic 45.0%
AAGGCAAATATCACGTCCCG+AGG + chr4.1:67979575-67979594 MS.gene48646:intron 50.0%
AGAGGGAGTGAAGTTGTGTC+TGG - chr4.1:67980182-67980201 None:intergenic 50.0%
CCAAGACGATCAACCATGTC+TGG - chr4.1:67980386-67980405 None:intergenic 50.0%
CCAGACATGGTTGATCGTCT+TGG + chr4.1:67980383-67980402 MS.gene48646:CDS 50.0%
GACATGGTTGATCGTCTTGG+TGG + chr4.1:67980386-67980405 MS.gene48646:CDS 50.0%
! CGATCAACCATGTCTGGAAC+AGG - chr4.1:67980380-67980399 None:intergenic 50.0%
!! ACTCCAGGTGGTGCTTTTTG+TGG - chr4.1:67979257-67979276 None:intergenic 50.0%
!!! TGCTCTTTTGGTTTAGCCGG+TGG - chr4.1:67979680-67979699 None:intergenic 50.0%
!! GGACCACAAAAAGCACCACC+TGG + chr4.1:67979251-67979270 MS.gene48646:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 67979095 67980520 67979095 ID=MS.gene48646
chr4.1 mRNA 67979095 67980520 67979095 ID=MS.gene48646.t1;Parent=MS.gene48646
chr4.1 exon 67979095 67979423 67979095 ID=MS.gene48646.t1.exon1;Parent=MS.gene48646.t1
chr4.1 CDS 67979095 67979423 67979095 ID=cds.MS.gene48646.t1;Parent=MS.gene48646.t1
chr4.1 exon 67980103 67980520 67980103 ID=MS.gene48646.t1.exon2;Parent=MS.gene48646.t1
chr4.1 CDS 67980103 67980520 67980103 ID=cds.MS.gene48646.t1;Parent=MS.gene48646.t1
Gene Sequence

>MS.gene48646

ATGGCTTCAACAGCAACACCTTCTCCACAAATTCTACTATTCAATCCAATCTTAGGAAACACCAAAACAATAAGAAGAATAAGATTTTTTAGGATAACAACAACCTCAAACTCTCACTACAGTTTGCAAGTTCATGCTTCTACTAAGGACACTCAAGGACCACAAAAAGCACCACCTGGAGTTGACACAAGAATCCATTGGGAGAATGATGATGAAGGTTGGATTGGAGGTAGCACCAAACAGCAACAAACAAATGCAGAGGAGAAACCTAAGAAGCTTTTGGGTGAAGACTTTGCTGATTTGCTCAATTTTCAAGGTTCTCATTATGAGTGAGTTTCTTTATAACCGTTTCCTACAAAAAGTATAAAACACCAAAAACCACTTCGATTTTCTATTAGGTCCGATTGTAAACTGAATTTTGCCTTGAAATTCAATTTTAAACTAAAGTTCGTATAAGTATGTGTTTGGACTAGCTTTTACAAGGCAAATATCACGTCCCGAGGCAAGCTATACGTGAAAATCCCGAAGCTACAAAAGCAAGCTTTTGTAAAAACGTGAAAATTTACCGTGATTTTATAAAAACCACCGGCTAAACCAAAAGAGCACTAAGTATAACTAAAGCATTTCAGCTAATGTTGAATAGTTTGGATACGACTTGAGTTCAATTGTTGGCTAAAAGACTAAAACAACTATTAGTCGGACTTTACTTCCTTTTTGATCAAACTCTGACATAATCGGTACATGTTTTGGGTTAAGATAAAAATATTAAGAGTTTGTTGCATTGTAAACTGATTTTTTCAGAATGAAGTTCATTTAAACTTAAGTTTAGTCAAGATACTATTATTAAATTCTGTTTTTCTTCAGGTAACATGAGAATTTTCTTTTCGTTTTAAAAAAAGAAAAACTGGTGCATAAGTTGCCAACTTACTAGTTACCAGAGATTTGTATCATGAGAAAACTTGTTTATTTAAATGACAAGAGTTACTGAATAATGGAATATTTGTGCAGATTCTTAGGAATATCACCAGAAGCTGATGTAGAAGCAATCAAAGTAGCATTCAGGAAACTTTCAAAGGAGTATCATCCAGACACAACTTCACTCCCTCTAAAAACAGCCTCAGAAAAATTCATGAAATTAAAAGAAGTTTACACTGTTCTGAGCAATGAAGAAAGTAGAAGATTCTATGATTGGACCCTTGCTCAAGAAGTTGCAAGTCGCCAACAAGAGAAACTGAAAATAAAATTAGAGGATCCTTATGAGGTAGCCTTGAAGAATTATGAACCTGTTCCAGACATGGTTGATCGTCTTGGTGGGAAGAATATGAAGCTTAGTGATCAAGCTGTTTCTGCTATCACTATTGATATTTTTATCATTATTTTTTCCATATGCTGTATCACTCATGTAGTTTTCTTTAAAGAATACTGA

Protein sequence

>MS.gene48646.t1

MASTATPSPQILLFNPILGNTKTIRRIRFFRITTTSNSHYSLQVHASTKDTQGPQKAPPGVDTRIHWENDDEGWIGGSTKQQQTNAEEKPKKLLGEDFADLLNFQGSHYEFLGISPEADVEAIKVAFRKLSKEYHPDTTSLPLKTASEKFMKLKEVYTVLSNEESRRFYDWTLAQEVASRQQEKLKIKLEDPYEVALKNYEPVPDMVDRLGGKNMKLSDQAVSAITIDIFIIIFSICCITHVVFFKEY