AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48649


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48649.t1 MTR_4g032805 96.371 248 8 1 1 248 23 269 0.0 498
MS.gene48649.t1 MTR_2g097690 50.000 62 29 1 109 170 77 136 3.52e-12 63.9
MS.gene48649.t1 MTR_5g022180 36.264 91 55 2 87 176 53 141 7.03e-12 62.4
MS.gene48649.t1 MTR_2g097690 50.000 64 30 1 107 170 75 136 1.34e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48649.t1 AT4G09350 59.389 229 82 3 20 247 28 246 8.25e-96 281
MS.gene48649.t1 AT5G59610 49.231 65 31 1 105 169 71 133 2.68e-11 62.4
MS.gene48649.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48649.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48649.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2
MS.gene48649.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2

Find 39 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACCCAAAAGCTTCTT+AGG 0.196506 4.3:-67611583 None:intergenic
CTACTATTCAATCCAATCTT+AGG 0.223430 4.3:+67611349 MS.gene48649:CDS
GAATATTTGTGCAGATTCTT+AGG 0.256272 4.3:+67612310 MS.gene48649:intron
TCTTATTCTTCTTATTGTTT+TGG 0.269689 4.3:-67611377 None:intergenic
GGAGAAACCTAAGAAGCTTT+TGG 0.298843 4.3:+67611576 MS.gene48649:CDS
TGCATTTGTTTGTTGCTGTT+TGG 0.332246 4.3:-67611551 None:intergenic
ATTGCTTCTACATCAGCTTC+TGG 0.336747 4.3:-67612340 None:intergenic
GCAAGTTCATGCTTCTACTA+AGG 0.365942 4.3:+67611441 MS.gene48649:CDS
GAAACTGAAAATAAAATTAG+AGG 0.366458 4.3:+67612543 MS.gene48649:CDS
AGAGGGAGTGAAGTTGTGTC+TGG 0.393350 4.3:-67612400 None:intergenic
AAGCAATCAAAGTAGCATTC+AGG 0.395851 4.3:+67612356 MS.gene48649:CDS
AAAGTAGAAGATTCTATGAT+TGG 0.404469 4.3:+67612485 MS.gene48649:CDS
TTCTTCAAGGCTACCTCATA+AGG 0.409509 4.3:-67612568 None:intergenic
CAAACTGTAGTGAGAGTTTG+AGG 0.431351 4.3:-67611419 None:intergenic
GGATTGAATAGTAGAATTTG+TGG 0.442017 4.3:-67611340 None:intergenic
AACAGGTTCATAATTCTTCA+AGG 0.442631 4.3:-67612581 None:intergenic
CCAAGACGATCAACCATGTC+TGG 0.451377 4.3:-67612604 None:intergenic
CGATCAACCATGTCTGGAAC+AGG 0.460674 4.3:-67612598 None:intergenic
GATGATGAAGGTTGGATTGG+AGG 0.480496 4.3:+67611523 MS.gene48649:CDS
AATAGTAGAATTTGTGGAGA+AGG 0.498829 4.3:-67611334 None:intergenic
AAAATTAGAGGATCCTTATG+AGG 0.498846 4.3:+67612555 MS.gene48649:CDS
TACATGAGTGATACAGCATA+TGG 0.500284 4.3:-67612698 None:intergenic
AATGATGATGAAGGTTGGAT+TGG 0.510553 4.3:+67611520 MS.gene48649:CDS
TGGATTCTTGTGTCAACTCC+AGG 0.515932 4.3:-67611490 None:intergenic
GGGAGAATGATGATGAAGGT+TGG 0.524754 4.3:+67611515 MS.gene48649:CDS
CTTCATCATCATTCTCCCAA+TGG 0.525735 4.3:-67611510 None:intergenic
GACTTGCAACTTCTTGAGCA+AGG 0.527830 4.3:-67612510 None:intergenic
GACATGGTTGATCGTCTTGG+TGG 0.530101 4.3:+67612607 MS.gene48649:CDS
CCAGACATGGTTGATCGTCT+TGG 0.532935 4.3:+67612604 MS.gene48649:CDS
AGTTGACACAAGAATCCATT+GGG 0.545872 4.3:+67611495 MS.gene48649:CDS
TTATGAACCTGTTCCAGACA+TGG 0.570153 4.3:+67612591 MS.gene48649:CDS
ACTTGCAACTTCTTGAGCAA+GGG 0.574767 4.3:-67612509 None:intergenic
AGCATTCAGGAAACTTTCAA+AGG 0.585269 4.3:+67612369 MS.gene48649:CDS
ACATGGTTGATCGTCTTGGT+GGG 0.606623 4.3:+67612608 MS.gene48649:CDS
CATTGGGAGAATGATGATGA+AGG 0.623650 4.3:+67611511 MS.gene48649:CDS
GAGTTGACACAAGAATCCAT+TGG 0.644255 4.3:+67611494 MS.gene48649:CDS
ACAGCAACAAACAAATGCAG+AGG 0.656916 4.3:+67611555 MS.gene48649:CDS
GCTTCTACTAAGGACACTCA+AGG 0.674014 4.3:+67611451 MS.gene48649:CDS
ATTCTTGTGTCAACTCCAGG+TGG 0.739348 4.3:-67611487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGAAGAATAAGATTTTTT+AGG + chr4.3:67611386-67611405 MS.gene48649:CDS 15.0%
!!! TAGTTTAAAATTGAATTTCA+AGG - chr4.3:67611740-67611759 None:intergenic 15.0%
!! GAAACTGAAAATAAAATTAG+AGG + chr4.3:67612543-67612562 MS.gene48649:CDS 20.0%
!!! ATTAAATTCTGTTTTTCTTC+AGG + chr4.3:67612158-67612177 MS.gene48649:intron 20.0%
!!! CGTTTTAAAAAAAGAAAAAC+TGG + chr4.3:67612201-67612220 MS.gene48649:intron 20.0%
!!! TAATTTCATGAATTTTTCTG+AGG - chr4.3:67612434-67612453 None:intergenic 20.0%
!!! TCTTATTCTTCTTATTGTTT+TGG - chr4.3:67611380-67611399 None:intergenic 20.0%
!!! TTTATACTTTTTGTAGGAAA+CGG - chr4.3:67611664-67611683 None:intergenic 20.0%
! AAAGTAGAAGATTCTATGAT+TGG + chr4.3:67612485-67612504 MS.gene48649:CDS 25.0%
! AGACTAAAACAACTATTAGT+CGG + chr4.3:67611993-67612012 MS.gene48649:intron 25.0%
!! TTATTTTCAGTTTCTCTTGT+TGG - chr4.3:67612537-67612556 None:intergenic 25.0%
!!! TGGTGTTTTATACTTTTTGT+AGG - chr4.3:67611670-67611689 None:intergenic 25.0%
AAAATTAGAGGATCCTTATG+AGG + chr4.3:67612555-67612574 MS.gene48649:CDS 30.0%
AACAGGTTCATAATTCTTCA+AGG - chr4.3:67612584-67612603 None:intergenic 30.0%
AATAGTAGAATTTGTGGAGA+AGG - chr4.3:67611337-67611356 None:intergenic 30.0%
AGTTCGTATAAGTATGTGTT+TGG + chr4.3:67611761-67611780 MS.gene48649:intron 30.0%
ATGTCAGAGTTTGATCAAAA+AGG - chr4.3:67612028-67612047 None:intergenic 30.0%
CTACTATTCAATCCAATCTT+AGG + chr4.3:67611349-67611368 MS.gene48649:CDS 30.0%
GAATATTTGTGCAGATTCTT+AGG + chr4.3:67612310-67612329 MS.gene48649:intron 30.0%
GGATTGAATAGTAGAATTTG+TGG - chr4.3:67611343-67611362 None:intergenic 30.0%
GGCAAAATTCAGTTTACAAT+CGG - chr4.3:67611719-67611738 None:intergenic 30.0%
TCAGCTAATGTTGAATAGTT+TGG + chr4.3:67611941-67611960 MS.gene48649:intron 30.0%
TGACAAGAGTTACTGAATAA+TGG + chr4.3:67612288-67612307 MS.gene48649:intron 30.0%
TGATCAAACTCTGACATAAT+CGG + chr4.3:67612031-67612050 MS.gene48649:intron 30.0%
TTTCTCATGATACAAATCTC+TGG - chr4.3:67612253-67612272 None:intergenic 30.0%
! AACCACTTCGATTTTCTATT+AGG + chr4.3:67611692-67611711 MS.gene48649:intron 30.0%
! ACATAATCGGTACATGTTTT+GGG + chr4.3:67612044-67612063 MS.gene48649:intron 30.0%
! AGTTATACTTAGTGCTCTTT+TGG - chr4.3:67611913-67611932 None:intergenic 30.0%
! CGGTGGTTTTTATAAAATCA+CGG - chr4.3:67611884-67611903 None:intergenic 30.0%
! CGTGATTTTATAAAAACCAC+CGG + chr4.3:67611882-67611901 MS.gene48649:intron 30.0%
! TAGAAAATCGAAGTGGTTTT+TGG - chr4.3:67611690-67611709 None:intergenic 30.0%
AAGCAATCAAAGTAGCATTC+AGG + chr4.3:67612356-67612375 MS.gene48649:CDS 35.0%
AGCATTCAGGAAACTTTCAA+AGG + chr4.3:67612369-67612388 MS.gene48649:CDS 35.0%
AGTTGACACAAGAATCCATT+GGG + chr4.3:67611495-67611514 MS.gene48649:CDS 35.0%
ATCTCTGGTAACTAGTAAGT+TGG - chr4.3:67612238-67612257 None:intergenic 35.0%
GACCTAATAGAAAATCGAAG+TGG - chr4.3:67611697-67611716 None:intergenic 35.0%
TACATGAGTGATACAGCATA+TGG - chr4.3:67612701-67612720 None:intergenic 35.0%
TACGACTTGAGTTCAATTGT+TGG + chr4.3:67611965-67611984 MS.gene48649:intron 35.0%
! AATGATGATGAAGGTTGGAT+TGG + chr4.3:67611520-67611539 MS.gene48649:CDS 35.0%
! GACATAATCGGTACATGTTT+TGG + chr4.3:67612043-67612062 MS.gene48649:intron 35.0%
! GAGAAACCTAAGAAGCTTTT+GGG + chr4.3:67611577-67611596 MS.gene48649:CDS 35.0%
! TGCATTTGTTTGTTGCTGTT+TGG - chr4.3:67611554-67611573 None:intergenic 35.0%
!! AAAGCTTGCTTTTGTAGCTT+CGG - chr4.3:67611841-67611860 None:intergenic 35.0%
!!! GCTGATTTGCTCAATTTTCA+AGG + chr4.3:67611610-67611629 MS.gene48649:CDS 35.0%
!!! GTTTTGGTGTTTCCTAAGAT+TGG - chr4.3:67611364-67611383 None:intergenic 35.0%
!!! TGTTTGGACTAGCTTTTACA+AGG + chr4.3:67611777-67611796 MS.gene48649:intron 35.0%
!!! TTTCTGAGGCTGTTTTTAGA+GGG - chr4.3:67612420-67612439 None:intergenic 35.0%
!!! TTTTCTGAGGCTGTTTTTAG+AGG - chr4.3:67612421-67612440 None:intergenic 35.0%
ACAGCAACAAACAAATGCAG+AGG + chr4.3:67611555-67611574 MS.gene48649:CDS 40.0%
ACTTGCAACTTCTTGAGCAA+GGG - chr4.3:67612512-67612531 None:intergenic 40.0%
ATTGCTTCTACATCAGCTTC+TGG - chr4.3:67612343-67612362 None:intergenic 40.0%
CAAACTGTAGTGAGAGTTTG+AGG - chr4.3:67611422-67611441 None:intergenic 40.0%
CTTCATCATCATTCTCCCAA+TGG - chr4.3:67611513-67611532 None:intergenic 40.0%
GAGTTGACACAAGAATCCAT+TGG + chr4.3:67611494-67611513 MS.gene48649:CDS 40.0%
GCAAGTTCATGCTTCTACTA+AGG + chr4.3:67611441-67611460 MS.gene48649:CDS 40.0%
TCTTCACCCAAAAGCTTCTT+AGG - chr4.3:67611586-67611605 None:intergenic 40.0%
TTATGAACCTGTTCCAGACA+TGG + chr4.3:67612591-67612610 MS.gene48649:CDS 40.0%
TTCTTCAAGGCTACCTCATA+AGG - chr4.3:67612571-67612590 None:intergenic 40.0%
! CATTGGGAGAATGATGATGA+AGG + chr4.3:67611511-67611530 MS.gene48649:CDS 40.0%
! GGAGAAACCTAAGAAGCTTT+TGG + chr4.3:67611576-67611595 MS.gene48649:CDS 40.0%
!! AAGCTTGCTTTTGTAGCTTC+GGG - chr4.3:67611840-67611859 None:intergenic 40.0%
!! TTTTCACGTATAGCTTGCCT+CGG - chr4.3:67611816-67611835 None:intergenic 40.0%
!!! TAGTGCTCTTTTGGTTTAGC+CGG - chr4.3:67611904-67611923 None:intergenic 40.0%
ACATGGTTGATCGTCTTGGT+GGG + chr4.3:67612608-67612627 MS.gene48649:CDS 45.0%
ATTCTTGTGTCAACTCCAGG+TGG - chr4.3:67611490-67611509 None:intergenic 45.0%
GACTTGCAACTTCTTGAGCA+AGG - chr4.3:67612513-67612532 None:intergenic 45.0%
GCTTCTACTAAGGACACTCA+AGG + chr4.3:67611451-67611470 MS.gene48649:CDS 45.0%
TGGATTCTTGTGTCAACTCC+AGG - chr4.3:67611493-67611512 None:intergenic 45.0%
! GATGATGAAGGTTGGATTGG+AGG + chr4.3:67611523-67611542 MS.gene48649:CDS 45.0%
! GGGAGAATGATGATGAAGGT+TGG + chr4.3:67611515-67611534 MS.gene48649:CDS 45.0%
! TTTCACGTATAGCTTGCCTC+GGG - chr4.3:67611815-67611834 None:intergenic 45.0%
AAGGCAAATATCACGTCCCG+AGG + chr4.3:67611796-67611815 MS.gene48649:intron 50.0%
AGAGGGAGTGAAGTTGTGTC+TGG - chr4.3:67612403-67612422 None:intergenic 50.0%
CCAAGACGATCAACCATGTC+TGG - chr4.3:67612607-67612626 None:intergenic 50.0%
CCAGACATGGTTGATCGTCT+TGG + chr4.3:67612604-67612623 MS.gene48649:CDS 50.0%
GACATGGTTGATCGTCTTGG+TGG + chr4.3:67612607-67612626 MS.gene48649:CDS 50.0%
! CGATCAACCATGTCTGGAAC+AGG - chr4.3:67612601-67612620 None:intergenic 50.0%
!! ACTCCAGGTGGTGCTTTTTG+TGG - chr4.3:67611478-67611497 None:intergenic 50.0%
!!! TGCTCTTTTGGTTTAGCCGG+TGG - chr4.3:67611901-67611920 None:intergenic 50.0%
!! GGACCACAAAAAGCACCACC+TGG + chr4.3:67611472-67611491 MS.gene48649:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 67611316 67612741 67611316 ID=MS.gene48649
chr4.3 mRNA 67611316 67612741 67611316 ID=MS.gene48649.t1;Parent=MS.gene48649
chr4.3 exon 67611316 67611644 67611316 ID=MS.gene48649.t1.exon1;Parent=MS.gene48649.t1
chr4.3 CDS 67611316 67611644 67611316 ID=cds.MS.gene48649.t1;Parent=MS.gene48649.t1
chr4.3 exon 67612324 67612741 67612324 ID=MS.gene48649.t1.exon2;Parent=MS.gene48649.t1
chr4.3 CDS 67612324 67612741 67612324 ID=cds.MS.gene48649.t1;Parent=MS.gene48649.t1
Gene Sequence

>MS.gene48649

ATGGCTTCAACAGCAACACCTTCTCCACAAATTCTACTATTCAATCCAATCTTAGGAAACACCAAAACAATAAGAAGAATAAGATTTTTTAGGATAACAACAACCTCAAACTCTCACTACAGTTTGCAAGTTCATGCTTCTACTAAGGACACTCAAGGACCACAAAAAGCACCACCTGGAGTTGACACAAGAATCCATTGGGAGAATGATGATGAAGGTTGGATTGGAGGTAGCACCAAACAGCAACAAACAAATGCAGAGGAGAAACCTAAGAAGCTTTTGGGTGAAGACTTTGCTGATTTGCTCAATTTTCAAGGTTCTCATTATGAGTGAGTTTCTTTATAACCGTTTCCTACAAAAAGTATAAAACACCAAAAACCACTTCGATTTTCTATTAGGTCCGATTGTAAACTGAATTTTGCCTTGAAATTCAATTTTAAACTAAAGTTCGTATAAGTATGTGTTTGGACTAGCTTTTACAAGGCAAATATCACGTCCCGAGGCAAGCTATACGTGAAAATCCCGAAGCTACAAAAGCAAGCTTTTGTAAAAACGTGAAAATTTACCGTGATTTTATAAAAACCACCGGCTAAACCAAAAGAGCACTAAGTATAACTAAAGCATTTCAGCTAATGTTGAATAGTTTGGATACGACTTGAGTTCAATTGTTGGCTAAAAGACTAAAACAACTATTAGTCGGACTTTACTTCCTTTTTGATCAAACTCTGACATAATCGGTACATGTTTTGGGTTAAGATAAAAATATTAAGAGTTTGTTGCATTGTAAACTGATTTTTTCAGAATGAAGTTCATTTAAACTTAAGTTTAGTCAAGATACTATTATTAAATTCTGTTTTTCTTCAGGTAACATGAGAATTTTCTTTTCGTTTTAAAAAAAGAAAAACTGGTGCATAAGTTGCCAACTTACTAGTTACCAGAGATTTGTATCATGAGAAAACTTGTTTATTTAAATGACAAGAGTTACTGAATAATGGAATATTTGTGCAGATTCTTAGGAATATCACCAGAAGCTGATGTAGAAGCAATCAAAGTAGCATTCAGGAAACTTTCAAAGGAGTATCATCCAGACACAACTTCACTCCCTCTAAAAACAGCCTCAGAAAAATTCATGAAATTAAAAGAAGTTTACACTGTTCTGAGCAATGAAGAAAGTAGAAGATTCTATGATTGGACCCTTGCTCAAGAAGTTGCAAGTCGCCAACAAGAGAAACTGAAAATAAAATTAGAGGATCCTTATGAGGTAGCCTTGAAGAATTATGAACCTGTTCCAGACATGGTTGATCGTCTTGGTGGGAAGAATATGAAGCTTAGTGATCAAGCTGTTTCTGCTATCACTATTGATATTTTTATCATTATTTTTTCCATATGCTGTATCACTCATGTAGTTTTCTTTAAAGAATACTGA

Protein sequence

>MS.gene48649.t1

MASTATPSPQILLFNPILGNTKTIRRIRFFRITTTSNSHYSLQVHASTKDTQGPQKAPPGVDTRIHWENDDEGWIGGSTKQQQTNAEEKPKKLLGEDFADLLNFQGSHYEFLGISPEADVEAIKVAFRKLSKEYHPDTTSLPLKTASEKFMKLKEVYTVLSNEESRRFYDWTLAQEVASRQQEKLKIKLEDPYEVALKNYEPVPDMVDRLGGKNMKLSDQAVSAITIDIFIIIFSICCITHVVFFKEY