AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83251


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83251.t1 MTR_4g032805 96.371 248 8 1 1 248 23 269 0.0 498
MS.gene83251.t1 MTR_2g097690 50.000 62 29 1 109 170 77 136 3.52e-12 63.9
MS.gene83251.t1 MTR_5g022180 36.264 91 55 2 87 176 53 141 7.03e-12 62.4
MS.gene83251.t1 MTR_2g097690 50.000 64 30 1 107 170 75 136 1.34e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83251.t1 AT4G09350 59.389 229 82 3 20 247 28 246 8.25e-96 281
MS.gene83251.t1 AT5G59610 49.231 65 31 1 105 169 71 133 2.68e-11 62.4
MS.gene83251.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene83251.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene83251.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2
MS.gene83251.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2

Find 39 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACCCAAAAGCTTCTT+AGG 0.196506 4.3:-67598451 None:intergenic
CTACTATTCAATCCAATCTT+AGG 0.223430 4.3:+67598217 MS.gene83251:CDS
GAATATTTGTGCAGATTCTT+AGG 0.256272 4.3:+67599178 MS.gene83251:intron
TCTTATTCTTCTTATTGTTT+TGG 0.269689 4.3:-67598245 None:intergenic
GGAGAAACCTAAGAAGCTTT+TGG 0.298843 4.3:+67598444 MS.gene83251:CDS
TGCATTTGTTTGTTGCTGTT+TGG 0.332246 4.3:-67598419 None:intergenic
ATTGCTTCTACATCAGCTTC+TGG 0.336747 4.3:-67599208 None:intergenic
GCAAGTTCATGCTTCTACTA+AGG 0.365942 4.3:+67598309 MS.gene83251:CDS
GAAACTGAAAATAAAATTAG+AGG 0.366458 4.3:+67599411 MS.gene83251:CDS
AGAGGGAGTGAAGTTGTGTC+TGG 0.393350 4.3:-67599268 None:intergenic
AAGCAATCAAAGTAGCATTC+AGG 0.395851 4.3:+67599224 MS.gene83251:CDS
AAAGTAGAAGATTCTATGAT+TGG 0.404469 4.3:+67599353 MS.gene83251:CDS
TTCTTCAAGGCTACCTCATA+AGG 0.409509 4.3:-67599436 None:intergenic
CAAACTGTAGTGAGAGTTTG+AGG 0.431351 4.3:-67598287 None:intergenic
GGATTGAATAGTAGAATTTG+TGG 0.442017 4.3:-67598208 None:intergenic
AACAGGTTCATAATTCTTCA+AGG 0.442631 4.3:-67599449 None:intergenic
CCAAGACGATCAACCATGTC+TGG 0.451377 4.3:-67599472 None:intergenic
CGATCAACCATGTCTGGAAC+AGG 0.460674 4.3:-67599466 None:intergenic
GATGATGAAGGTTGGATTGG+AGG 0.480496 4.3:+67598391 MS.gene83251:CDS
AATAGTAGAATTTGTGGAGA+AGG 0.498829 4.3:-67598202 None:intergenic
AAAATTAGAGGATCCTTATG+AGG 0.498846 4.3:+67599423 MS.gene83251:CDS
TACATGAGTGATACAGCATA+TGG 0.500284 4.3:-67599566 None:intergenic
AATGATGATGAAGGTTGGAT+TGG 0.510553 4.3:+67598388 MS.gene83251:CDS
TGGATTCTTGTGTCAACTCC+AGG 0.515932 4.3:-67598358 None:intergenic
GGGAGAATGATGATGAAGGT+TGG 0.524754 4.3:+67598383 MS.gene83251:CDS
CTTCATCATCATTCTCCCAA+TGG 0.525735 4.3:-67598378 None:intergenic
GACTTGCAACTTCTTGAGCA+AGG 0.527830 4.3:-67599378 None:intergenic
GACATGGTTGATCGTCTTGG+TGG 0.530101 4.3:+67599475 MS.gene83251:CDS
CCAGACATGGTTGATCGTCT+TGG 0.532935 4.3:+67599472 MS.gene83251:CDS
AGTTGACACAAGAATCCATT+GGG 0.545872 4.3:+67598363 MS.gene83251:CDS
TTATGAACCTGTTCCAGACA+TGG 0.570153 4.3:+67599459 MS.gene83251:CDS
ACTTGCAACTTCTTGAGCAA+GGG 0.574767 4.3:-67599377 None:intergenic
AGCATTCAGGAAACTTTCAA+AGG 0.585269 4.3:+67599237 MS.gene83251:CDS
ACATGGTTGATCGTCTTGGT+GGG 0.606623 4.3:+67599476 MS.gene83251:CDS
CATTGGGAGAATGATGATGA+AGG 0.623650 4.3:+67598379 MS.gene83251:CDS
GAGTTGACACAAGAATCCAT+TGG 0.644255 4.3:+67598362 MS.gene83251:CDS
ACAGCAACAAACAAATGCAG+AGG 0.656916 4.3:+67598423 MS.gene83251:CDS
GCTTCTACTAAGGACACTCA+AGG 0.674014 4.3:+67598319 MS.gene83251:CDS
ATTCTTGTGTCAACTCCAGG+TGG 0.739348 4.3:-67598355 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGAAGAATAAGATTTTTT+AGG + chr4.3:67598254-67598273 MS.gene83251:CDS 15.0%
!!! TAGTTTAAAATTGAATTTCA+AGG - chr4.3:67598608-67598627 None:intergenic 15.0%
!! GAAACTGAAAATAAAATTAG+AGG + chr4.3:67599411-67599430 MS.gene83251:CDS 20.0%
!!! ATTAAATTCTGTTTTTCTTC+AGG + chr4.3:67599026-67599045 MS.gene83251:intron 20.0%
!!! CGTTTTAAAAAAAGAAAAAC+TGG + chr4.3:67599069-67599088 MS.gene83251:intron 20.0%
!!! TAATTTCATGAATTTTTCTG+AGG - chr4.3:67599302-67599321 None:intergenic 20.0%
!!! TCTTATTCTTCTTATTGTTT+TGG - chr4.3:67598248-67598267 None:intergenic 20.0%
!!! TTTATACTTTTTGTAGGAAA+CGG - chr4.3:67598532-67598551 None:intergenic 20.0%
! AAAGTAGAAGATTCTATGAT+TGG + chr4.3:67599353-67599372 MS.gene83251:CDS 25.0%
! AGACTAAAACAACTATTAGT+CGG + chr4.3:67598861-67598880 MS.gene83251:intron 25.0%
!! TTATTTTCAGTTTCTCTTGT+TGG - chr4.3:67599405-67599424 None:intergenic 25.0%
!!! TGGTGTTTTATACTTTTTGT+AGG - chr4.3:67598538-67598557 None:intergenic 25.0%
AAAATTAGAGGATCCTTATG+AGG + chr4.3:67599423-67599442 MS.gene83251:CDS 30.0%
AACAGGTTCATAATTCTTCA+AGG - chr4.3:67599452-67599471 None:intergenic 30.0%
AATAGTAGAATTTGTGGAGA+AGG - chr4.3:67598205-67598224 None:intergenic 30.0%
AGTTCGTATAAGTATGTGTT+TGG + chr4.3:67598629-67598648 MS.gene83251:intron 30.0%
ATGTCAGAGTTTGATCAAAA+AGG - chr4.3:67598896-67598915 None:intergenic 30.0%
CTACTATTCAATCCAATCTT+AGG + chr4.3:67598217-67598236 MS.gene83251:CDS 30.0%
GAATATTTGTGCAGATTCTT+AGG + chr4.3:67599178-67599197 MS.gene83251:intron 30.0%
GGATTGAATAGTAGAATTTG+TGG - chr4.3:67598211-67598230 None:intergenic 30.0%
GGCAAAATTCAGTTTACAAT+CGG - chr4.3:67598587-67598606 None:intergenic 30.0%
TCAGCTAATGTTGAATAGTT+TGG + chr4.3:67598809-67598828 MS.gene83251:intron 30.0%
TGACAAGAGTTACTGAATAA+TGG + chr4.3:67599156-67599175 MS.gene83251:intron 30.0%
TGATCAAACTCTGACATAAT+CGG + chr4.3:67598899-67598918 MS.gene83251:intron 30.0%
TTTCTCATGATACAAATCTC+TGG - chr4.3:67599121-67599140 None:intergenic 30.0%
! AACCACTTCGATTTTCTATT+AGG + chr4.3:67598560-67598579 MS.gene83251:intron 30.0%
! ACATAATCGGTACATGTTTT+GGG + chr4.3:67598912-67598931 MS.gene83251:intron 30.0%
! AGTTATACTTAGTGCTCTTT+TGG - chr4.3:67598781-67598800 None:intergenic 30.0%
! CGGTGGTTTTTATAAAATCA+CGG - chr4.3:67598752-67598771 None:intergenic 30.0%
! CGTGATTTTATAAAAACCAC+CGG + chr4.3:67598750-67598769 MS.gene83251:intron 30.0%
! TAGAAAATCGAAGTGGTTTT+TGG - chr4.3:67598558-67598577 None:intergenic 30.0%
AAGCAATCAAAGTAGCATTC+AGG + chr4.3:67599224-67599243 MS.gene83251:CDS 35.0%
AGCATTCAGGAAACTTTCAA+AGG + chr4.3:67599237-67599256 MS.gene83251:CDS 35.0%
AGTTGACACAAGAATCCATT+GGG + chr4.3:67598363-67598382 MS.gene83251:CDS 35.0%
ATCTCTGGTAACTAGTAAGT+TGG - chr4.3:67599106-67599125 None:intergenic 35.0%
GACCTAATAGAAAATCGAAG+TGG - chr4.3:67598565-67598584 None:intergenic 35.0%
TACATGAGTGATACAGCATA+TGG - chr4.3:67599569-67599588 None:intergenic 35.0%
TACGACTTGAGTTCAATTGT+TGG + chr4.3:67598833-67598852 MS.gene83251:intron 35.0%
! AATGATGATGAAGGTTGGAT+TGG + chr4.3:67598388-67598407 MS.gene83251:CDS 35.0%
! GACATAATCGGTACATGTTT+TGG + chr4.3:67598911-67598930 MS.gene83251:intron 35.0%
! GAGAAACCTAAGAAGCTTTT+GGG + chr4.3:67598445-67598464 MS.gene83251:CDS 35.0%
! TGCATTTGTTTGTTGCTGTT+TGG - chr4.3:67598422-67598441 None:intergenic 35.0%
!! AAAGCTTGCTTTTGTAGCTT+CGG - chr4.3:67598709-67598728 None:intergenic 35.0%
!!! GCTGATTTGCTCAATTTTCA+AGG + chr4.3:67598478-67598497 MS.gene83251:CDS 35.0%
!!! GTTTTGGTGTTTCCTAAGAT+TGG - chr4.3:67598232-67598251 None:intergenic 35.0%
!!! TGTTTGGACTAGCTTTTACA+AGG + chr4.3:67598645-67598664 MS.gene83251:intron 35.0%
!!! TTTCTGAGGCTGTTTTTAGA+GGG - chr4.3:67599288-67599307 None:intergenic 35.0%
!!! TTTTCTGAGGCTGTTTTTAG+AGG - chr4.3:67599289-67599308 None:intergenic 35.0%
ACAGCAACAAACAAATGCAG+AGG + chr4.3:67598423-67598442 MS.gene83251:CDS 40.0%
ACTTGCAACTTCTTGAGCAA+GGG - chr4.3:67599380-67599399 None:intergenic 40.0%
ATTGCTTCTACATCAGCTTC+TGG - chr4.3:67599211-67599230 None:intergenic 40.0%
CAAACTGTAGTGAGAGTTTG+AGG - chr4.3:67598290-67598309 None:intergenic 40.0%
CTTCATCATCATTCTCCCAA+TGG - chr4.3:67598381-67598400 None:intergenic 40.0%
GAGTTGACACAAGAATCCAT+TGG + chr4.3:67598362-67598381 MS.gene83251:CDS 40.0%
GCAAGTTCATGCTTCTACTA+AGG + chr4.3:67598309-67598328 MS.gene83251:CDS 40.0%
TCTTCACCCAAAAGCTTCTT+AGG - chr4.3:67598454-67598473 None:intergenic 40.0%
TTATGAACCTGTTCCAGACA+TGG + chr4.3:67599459-67599478 MS.gene83251:CDS 40.0%
TTCTTCAAGGCTACCTCATA+AGG - chr4.3:67599439-67599458 None:intergenic 40.0%
! CATTGGGAGAATGATGATGA+AGG + chr4.3:67598379-67598398 MS.gene83251:CDS 40.0%
! GGAGAAACCTAAGAAGCTTT+TGG + chr4.3:67598444-67598463 MS.gene83251:CDS 40.0%
!! AAGCTTGCTTTTGTAGCTTC+GGG - chr4.3:67598708-67598727 None:intergenic 40.0%
!! TTTTCACGTATAGCTTGCCT+CGG - chr4.3:67598684-67598703 None:intergenic 40.0%
!!! TAGTGCTCTTTTGGTTTAGC+CGG - chr4.3:67598772-67598791 None:intergenic 40.0%
ACATGGTTGATCGTCTTGGT+GGG + chr4.3:67599476-67599495 MS.gene83251:CDS 45.0%
ATTCTTGTGTCAACTCCAGG+TGG - chr4.3:67598358-67598377 None:intergenic 45.0%
GACTTGCAACTTCTTGAGCA+AGG - chr4.3:67599381-67599400 None:intergenic 45.0%
GCTTCTACTAAGGACACTCA+AGG + chr4.3:67598319-67598338 MS.gene83251:CDS 45.0%
TGGATTCTTGTGTCAACTCC+AGG - chr4.3:67598361-67598380 None:intergenic 45.0%
! GATGATGAAGGTTGGATTGG+AGG + chr4.3:67598391-67598410 MS.gene83251:CDS 45.0%
! GGGAGAATGATGATGAAGGT+TGG + chr4.3:67598383-67598402 MS.gene83251:CDS 45.0%
! TTTCACGTATAGCTTGCCTC+GGG - chr4.3:67598683-67598702 None:intergenic 45.0%
AAGGCAAATATCACGTCCCG+AGG + chr4.3:67598664-67598683 MS.gene83251:intron 50.0%
AGAGGGAGTGAAGTTGTGTC+TGG - chr4.3:67599271-67599290 None:intergenic 50.0%
CCAAGACGATCAACCATGTC+TGG - chr4.3:67599475-67599494 None:intergenic 50.0%
CCAGACATGGTTGATCGTCT+TGG + chr4.3:67599472-67599491 MS.gene83251:CDS 50.0%
GACATGGTTGATCGTCTTGG+TGG + chr4.3:67599475-67599494 MS.gene83251:CDS 50.0%
! CGATCAACCATGTCTGGAAC+AGG - chr4.3:67599469-67599488 None:intergenic 50.0%
!! ACTCCAGGTGGTGCTTTTTG+TGG - chr4.3:67598346-67598365 None:intergenic 50.0%
!!! TGCTCTTTTGGTTTAGCCGG+TGG - chr4.3:67598769-67598788 None:intergenic 50.0%
!! GGACCACAAAAAGCACCACC+TGG + chr4.3:67598340-67598359 MS.gene83251:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 67598184 67599609 67598184 ID=MS.gene83251
chr4.3 mRNA 67598184 67599609 67598184 ID=MS.gene83251.t1;Parent=MS.gene83251
chr4.3 exon 67598184 67598512 67598184 ID=MS.gene83251.t1.exon1;Parent=MS.gene83251.t1
chr4.3 CDS 67598184 67598512 67598184 ID=cds.MS.gene83251.t1;Parent=MS.gene83251.t1
chr4.3 exon 67599192 67599609 67599192 ID=MS.gene83251.t1.exon2;Parent=MS.gene83251.t1
chr4.3 CDS 67599192 67599609 67599192 ID=cds.MS.gene83251.t1;Parent=MS.gene83251.t1
Gene Sequence

>MS.gene83251

ATGGCTTCAACAGCAACACCTTCTCCACAAATTCTACTATTCAATCCAATCTTAGGAAACACCAAAACAATAAGAAGAATAAGATTTTTTAGGATAACAACAACCTCAAACTCTCACTACAGTTTGCAAGTTCATGCTTCTACTAAGGACACTCAAGGACCACAAAAAGCACCACCTGGAGTTGACACAAGAATCCATTGGGAGAATGATGATGAAGGTTGGATTGGAGGTAGCACCAAACAGCAACAAACAAATGCAGAGGAGAAACCTAAGAAGCTTTTGGGTGAAGACTTTGCTGATTTGCTCAATTTTCAAGGTTCTCATTATGAGTGAGTTTCTTTATAACCGTTTCCTACAAAAAGTATAAAACACCAAAAACCACTTCGATTTTCTATTAGGTCCGATTGTAAACTGAATTTTGCCTTGAAATTCAATTTTAAACTAAAGTTCGTATAAGTATGTGTTTGGACTAGCTTTTACAAGGCAAATATCACGTCCCGAGGCAAGCTATACGTGAAAATCCCGAAGCTACAAAAGCAAGCTTTTGTAAAAACGTGAAAATTTACCGTGATTTTATAAAAACCACCGGCTAAACCAAAAGAGCACTAAGTATAACTAAAGCATTTCAGCTAATGTTGAATAGTTTGGATACGACTTGAGTTCAATTGTTGGCTAAAAGACTAAAACAACTATTAGTCGGACTTTACTTCCTTTTTGATCAAACTCTGACATAATCGGTACATGTTTTGGGTTAAGATAAAAATATTAAGAGTTTGTTGCATTGTAAACTGATTTTTTCAGAATGAAGTTCATTTAAACTTAAGTTTAGTCAAGATACTATTATTAAATTCTGTTTTTCTTCAGGTAACATGAGAATTTTCTTTTCGTTTTAAAAAAAGAAAAACTGGTGCATAAGTTGCCAACTTACTAGTTACCAGAGATTTGTATCATGAGAAAACTTGTTTATTTAAATGACAAGAGTTACTGAATAATGGAATATTTGTGCAGATTCTTAGGAATATCACCAGAAGCTGATGTAGAAGCAATCAAAGTAGCATTCAGGAAACTTTCAAAGGAGTATCATCCAGACACAACTTCACTCCCTCTAAAAACAGCCTCAGAAAAATTCATGAAATTAAAAGAAGTTTACACTGTTCTGAGCAATGAAGAAAGTAGAAGATTCTATGATTGGACCCTTGCTCAAGAAGTTGCAAGTCGCCAACAAGAGAAACTGAAAATAAAATTAGAGGATCCTTATGAGGTAGCCTTGAAGAATTATGAACCTGTTCCAGACATGGTTGATCGTCTTGGTGGGAAGAATATGAAGCTTAGTGATCAAGCTGTTTCTGCTATCACTATTGATATTTTTATCATTATTTTTTCCATATGCTGTATCACTCATGTAGTTTTCTTTAAAGAATACTGA

Protein sequence

>MS.gene83251.t1

MASTATPSPQILLFNPILGNTKTIRRIRFFRITTTSNSHYSLQVHASTKDTQGPQKAPPGVDTRIHWENDDEGWIGGSTKQQQTNAEEKPKKLLGEDFADLLNFQGSHYEFLGISPEADVEAIKVAFRKLSKEYHPDTTSLPLKTASEKFMKLKEVYTVLSNEESRRFYDWTLAQEVASRQQEKLKIKLEDPYEVALKNYEPVPDMVDRLGGKNMKLSDQAVSAITIDIFIIIFSICCITHVVFFKEY