Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72986.t1 | XP_013457674.1 | 97.1 | 280 | 8 | 0 | 1 | 280 | 1 | 280 | 2.30E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72986.t1 | Q0WUQ1 | 51.5 | 235 | 111 | 1 | 34 | 265 | 41 | 275 | 3.1e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72986.t1 | A0A072V0P4 | 97.1 | 280 | 8 | 0 | 1 | 280 | 1 | 280 | 1.6e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene72986 | 0.882854 | 7.35E-71 | -1.69E-46 |
| MS.gene049159 | MS.gene72986 | 0.807113 | 5.92E-50 | -1.69E-46 |
| MS.gene049178 | MS.gene72986 | 0.809777 | 1.60E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene72986 | 0.804439 | 2.16E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene72986 | 0.869418 | 3.14E-66 | -1.69E-46 |
| MS.gene049368 | MS.gene72986 | 0.816236 | 6.14E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene72986 | 0.831857 | 1.32E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene72986 | 0.828315 | 9.63E-55 | -1.69E-46 |
| MS.gene049821 | MS.gene72986 | 0.833534 | 5.05E-56 | -1.69E-46 |
| MS.gene049850 | MS.gene72986 | 0.836497 | 9.04E-57 | -1.69E-46 |
| MS.gene049887 | MS.gene72986 | 0.832063 | 1.17E-55 | -1.69E-46 |
| MS.gene050253 | MS.gene72986 | 0.808616 | 2.84E-50 | -1.69E-46 |
| MS.gene05045 | MS.gene72986 | 0.821962 | 3.05E-53 | -1.69E-46 |
| MS.gene050551 | MS.gene72986 | 0.811709 | 6.11E-51 | -1.69E-46 |
| MS.gene050657 | MS.gene72986 | 0.802891 | 4.52E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene72986 | 0.819132 | 1.36E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene72986 | 0.827086 | 1.90E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene72986 | 0.83951 | 1.52E-57 | -1.69E-46 |
| MS.gene050901 | MS.gene72986 | 0.852596 | 4.16E-61 | -1.69E-46 |
| MS.gene051029 | MS.gene72986 | 0.802021 | 6.84E-49 | -1.69E-46 |
| MS.gene051102 | MS.gene72986 | 0.814454 | 1.53E-51 | -1.69E-46 |
| MS.gene051180 | MS.gene72986 | 0.800925 | 1.15E-48 | -1.69E-46 |
| MS.gene051198 | MS.gene72986 | 0.816313 | 5.90E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene72986 | 0.805073 | 1.59E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene72986 | 0.84435 | 7.99E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene72986 | 0.81864 | 1.76E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene72986 | 0.800296 | 1.54E-48 | -1.69E-46 |
| MS.gene051388 | MS.gene72986 | 0.806303 | 8.78E-50 | -1.69E-46 |
| MS.gene051490 | MS.gene72986 | 0.818864 | 1.57E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene72986 | 0.830405 | 2.99E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene72986 | 0.810892 | 9.19E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene72986 | 0.819994 | 8.67E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene72986 | 0.870576 | 1.31E-66 | -1.69E-46 |
| MS.gene051720 | MS.gene72986 | 0.876644 | 1.19E-68 | -1.69E-46 |
| MS.gene051744 | MS.gene72986 | 0.832822 | 7.59E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene72986 | 0.814916 | 1.21E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene72986 | 0.81472 | 1.33E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene72986 | 0.854057 | 1.59E-61 | -1.69E-46 |
| MS.gene052307 | MS.gene72986 | 0.825364 | 4.88E-54 | -1.69E-46 |
| MS.gene052335 | MS.gene72986 | 0.828597 | 8.23E-55 | -1.69E-46 |
| MS.gene052342 | MS.gene72986 | 0.804113 | 2.52E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene72986 | 0.854455 | 1.22E-61 | -1.69E-46 |
| MS.gene052514 | MS.gene72986 | 0.803255 | 3.80E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene72986 | 0.880825 | 3.99E-70 | -1.69E-46 |
| MS.gene052584 | MS.gene72986 | 0.808166 | 3.54E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene72986 | 0.820722 | 5.90E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene72986 | 0.844773 | 6.14E-59 | -1.69E-46 |
| MS.gene052673 | MS.gene72986 | -0.800994 | 1.11E-48 | -1.69E-46 |
| MS.gene052732 | MS.gene72986 | 0.825603 | 4.28E-54 | -1.69E-46 |
| MS.gene052773 | MS.gene72986 | 0.828761 | 7.51E-55 | -1.69E-46 |
| MS.gene052863 | MS.gene72986 | 0.819127 | 1.37E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene72986 | 0.821503 | 3.90E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene72986 | 0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene72986 | 0.832965 | 7.00E-56 | -1.69E-46 |
| MS.gene053143 | MS.gene72986 | 0.803602 | 3.22E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene72986 | 0.85842 | 8.34E-63 | -1.69E-46 |
| MS.gene053164 | MS.gene72986 | 0.809412 | 1.92E-50 | -1.69E-46 |
| MS.gene053171 | MS.gene72986 | 0.815439 | 9.24E-52 | -1.69E-46 |
| MS.gene053174 | MS.gene72986 | 0.804174 | 2.45E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene72986 | 0.842418 | 2.62E-58 | -1.69E-46 |
| MS.gene053581 | MS.gene72986 | 0.869526 | 2.90E-66 | -1.69E-46 |
| MS.gene053599 | MS.gene72986 | 0.816136 | 6.46E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene72986 | 0.806967 | 6.36E-50 | -1.69E-46 |
| MS.gene053786 | MS.gene72986 | 0.801457 | 8.92E-49 | -1.69E-46 |
| MS.gene054139 | MS.gene72986 | 0.814559 | 1.45E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene72986 | 0.806734 | 7.12E-50 | -1.69E-46 |
| MS.gene054550 | MS.gene72986 | 0.806494 | 8.01E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene72986 | 0.850015 | 2.23E-60 | -1.69E-46 |
| MS.gene05458 | MS.gene72986 | 0.850312 | 1.84E-60 | -1.69E-46 |
| MS.gene05462 | MS.gene72986 | 0.803734 | 3.03E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene72986 | 0.811878 | 5.62E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene72986 | 0.816871 | 4.42E-52 | -1.69E-46 |
| MS.gene055144 | MS.gene72986 | 0.828293 | 9.75E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene72986 | 0.801095 | 1.06E-48 | -1.69E-46 |
| MS.gene055310 | MS.gene72986 | 0.817989 | 2.48E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene72986 | 0.825346 | 4.93E-54 | -1.69E-46 |
| MS.gene055313 | MS.gene72986 | 0.840525 | 8.25E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene72986 | 0.872949 | 2.15E-67 | -1.69E-46 |
| MS.gene055466 | MS.gene72986 | 0.872126 | 4.04E-67 | -1.69E-46 |
| MS.gene055468 | MS.gene72986 | 0.874226 | 7.97E-68 | -1.69E-46 |
| MS.gene055469 | MS.gene72986 | 0.873628 | 1.27E-67 | -1.69E-46 |
| MS.gene055512 | MS.gene72986 | 0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene72986 | 0.864297 | 1.35E-64 | -1.69E-46 |
| MS.gene055537 | MS.gene72986 | 0.846904 | 1.62E-59 | -1.69E-46 |
| MS.gene055580 | MS.gene72986 | -0.833451 | 5.29E-56 | -1.69E-46 |
| MS.gene055818 | MS.gene72986 | 0.810847 | 9.40E-51 | -1.69E-46 |
| MS.gene055864 | MS.gene72986 | 0.801967 | 7.01E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene72986 | 0.807193 | 5.70E-50 | -1.69E-46 |
| MS.gene055891 | MS.gene72986 | 0.828873 | 7.06E-55 | -1.69E-46 |
| MS.gene055926 | MS.gene72986 | 0.834837 | 2.38E-56 | -1.69E-46 |
| MS.gene056005 | MS.gene72986 | 0.801852 | 7.40E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene72986 | -0.826014 | 3.42E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene72986 | 0.810364 | 1.20E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene72986 | 0.829726 | 4.38E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene72986 | 0.8335 | 5.15E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene72986 | 0.876015 | 1.95E-68 | -1.69E-46 |
| MS.gene056390 | MS.gene72986 | 0.83822 | 3.27E-57 | -1.69E-46 |
| MS.gene056507 | MS.gene72986 | 0.815244 | 1.02E-51 | -1.69E-46 |
| MS.gene056522 | MS.gene72986 | -0.80128 | 9.70E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene72986 | 0.803556 | 3.30E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene72986 | 0.8159 | 7.29E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene72986 | 0.807678 | 4.50E-50 | -1.69E-46 |
| MS.gene057294 | MS.gene72986 | 0.816654 | 4.95E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene72986 | 0.840148 | 1.04E-57 | -1.69E-46 |
| MS.gene058087 | MS.gene72986 | 0.815133 | 1.08E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene72986 | 0.813323 | 2.71E-51 | -1.69E-46 |
| MS.gene058360 | MS.gene72986 | 0.807835 | 4.16E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene72986 | 0.822484 | 2.31E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene72986 | 0.821327 | 4.28E-53 | -1.69E-46 |
| MS.gene058636 | MS.gene72986 | 0.816015 | 6.88E-52 | -1.69E-46 |
| MS.gene058639 | MS.gene72986 | 0.811346 | 7.33E-51 | -1.69E-46 |
| MS.gene058647 | MS.gene72986 | 0.805232 | 1.47E-49 | -1.69E-46 |
| MS.gene058668 | MS.gene72986 | 0.812393 | 4.34E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene72986 | 0.837603 | 4.72E-57 | -1.69E-46 |
| MS.gene058945 | MS.gene72986 | 0.805107 | 1.57E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene72986 | 0.825597 | 4.30E-54 | -1.69E-46 |
| MS.gene059171 | MS.gene72986 | 0.836867 | 7.28E-57 | -1.69E-46 |
| MS.gene059227 | MS.gene72986 | 0.808099 | 3.66E-50 | -1.69E-46 |
| MS.gene059283 | MS.gene72986 | 0.807509 | 4.88E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene72986 | 0.815555 | 8.71E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene72986 | 0.823425 | 1.39E-53 | -1.69E-46 |
| MS.gene059713 | MS.gene72986 | 0.811207 | 7.86E-51 | -1.69E-46 |
| MS.gene059725 | MS.gene72986 | 0.833677 | 4.65E-56 | -1.69E-46 |
| MS.gene059842 | MS.gene72986 | 0.810099 | 1.36E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene72986 | 0.801622 | 8.25E-49 | -1.69E-46 |
| MS.gene059965 | MS.gene72986 | 0.845928 | 2.99E-59 | -1.69E-46 |
| MS.gene060519 | MS.gene72986 | 0.828717 | 7.70E-55 | -1.69E-46 |
| MS.gene06052 | MS.gene72986 | 0.801319 | 9.52E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene72986 | 0.834675 | 2.61E-56 | -1.69E-46 |
| MS.gene060637 | MS.gene72986 | 0.826585 | 2.50E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene72986 | 0.823287 | 1.50E-53 | -1.69E-46 |
| MS.gene060884 | MS.gene72986 | 0.800121 | 1.67E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene72986 | 0.802941 | 4.42E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene72986 | 0.833885 | 4.12E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene72986 | 0.841045 | 6.03E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72986.t1 | MTR_4g102740 | 97.143 | 280 | 8 | 0 | 1 | 280 | 1 | 280 | 0.0 | 543 |
| MS.gene72986.t1 | MTR_0086s0010 | 45.724 | 304 | 118 | 4 | 20 | 280 | 22 | 321 | 4.43e-79 | 243 |
| MS.gene72986.t1 | MTR_1g101440 | 47.902 | 286 | 114 | 5 | 28 | 280 | 18 | 301 | 4.39e-76 | 235 |
| MS.gene72986.t1 | MTR_2g018860 | 39.759 | 249 | 114 | 5 | 32 | 279 | 30 | 243 | 1.86e-53 | 174 |
| MS.gene72986.t1 | MTR_7g069610 | 44.554 | 202 | 111 | 1 | 32 | 232 | 19 | 220 | 1.59e-47 | 158 |
| MS.gene72986.t1 | MTR_4g017810 | 37.179 | 234 | 119 | 2 | 47 | 280 | 19 | 224 | 2.08e-40 | 140 |
| MS.gene72986.t1 | MTR_2g018860 | 39.109 | 202 | 88 | 4 | 79 | 279 | 34 | 201 | 1.68e-35 | 126 |
| MS.gene72986.t1 | MTR_6g022040 | 41.176 | 119 | 61 | 2 | 24 | 136 | 22 | 137 | 3.39e-21 | 87.8 |
| MS.gene72986.t1 | MTR_6g022020 | 43.810 | 105 | 57 | 1 | 32 | 136 | 38 | 140 | 3.77e-21 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72986.t1 | AT5G52060 | 46.545 | 275 | 119 | 3 | 34 | 280 | 41 | 315 | 1.63e-72 | 226 |
| MS.gene72986.t1 | AT5G62100 | 45.238 | 252 | 131 | 3 | 31 | 280 | 14 | 260 | 1.12e-66 | 209 |
| MS.gene72986.t1 | AT5G07220 | 44.615 | 260 | 132 | 4 | 32 | 280 | 21 | 279 | 2.37e-65 | 206 |
| MS.gene72986.t1 | AT5G62100 | 43.726 | 263 | 130 | 5 | 31 | 280 | 14 | 271 | 1.70e-63 | 201 |
| MS.gene72986.t1 | AT5G62100 | 51.087 | 184 | 89 | 1 | 31 | 214 | 14 | 196 | 1.02e-56 | 181 |
| MS.gene72986.t1 | AT5G62100 | 50.543 | 184 | 90 | 1 | 31 | 214 | 14 | 196 | 4.75e-56 | 180 |
| MS.gene72986.t1 | AT3G51780 | 35.985 | 264 | 124 | 5 | 31 | 279 | 9 | 242 | 6.22e-46 | 155 |
| MS.gene72986.t1 | AT5G62100 | 54.412 | 136 | 61 | 1 | 31 | 166 | 14 | 148 | 3.61e-44 | 147 |
| MS.gene72986.t1 | AT5G62100 | 54.412 | 136 | 61 | 1 | 31 | 166 | 14 | 148 | 3.61e-44 | 147 |
| MS.gene72986.t1 | AT5G14360 | 40.000 | 105 | 60 | 1 | 33 | 137 | 49 | 150 | 1.83e-20 | 86.3 |
| MS.gene72986.t1 | AT5G40630 | 39.815 | 108 | 62 | 2 | 26 | 132 | 50 | 155 | 1.03e-19 | 84.3 |
Find 40 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACATTGGATGAAATTTA+TGG | 0.207184 | 8.3:+19044504 | MS.gene72986:CDS |
| CTCTAAGGTTACTTCAATTA+AGG | 0.213411 | 8.3:-19044263 | None:intergenic |
| CTTAATTGAAGTAACCTTAG+AGG | 0.272826 | 8.3:+19044264 | MS.gene72986:CDS |
| CATGAGAACTGATATGAATT+TGG | 0.287339 | 8.3:-19043909 | None:intergenic |
| ATCAGTTCTCATGCAAGTTT+TGG | 0.294978 | 8.3:+19043919 | MS.gene72986:CDS |
| GGTTGACAAACTTGCCAAAA+AGG | 0.303391 | 8.3:+19044285 | MS.gene72986:CDS |
| GGCAAGTTTGTCAACCTCTA+AGG | 0.306312 | 8.3:-19044278 | None:intergenic |
| GAGTAGAGAAAGAAGGATAT+TGG | 0.354055 | 8.3:+19044201 | MS.gene72986:CDS |
| ATCAAAGTCAAAGTGAAGTA+TGG | 0.369232 | 8.3:+19043874 | MS.gene72986:CDS |
| CTTCACTTTGACTTTGATGA+TGG | 0.373301 | 8.3:-19043869 | None:intergenic |
| AATTCCCATTCAGCTACATT+TGG | 0.373777 | 8.3:-19043787 | None:intergenic |
| TTAATCAAAAGTTTCCCATT+TGG | 0.385906 | 8.3:-19044837 | None:intergenic |
| CAATCCAAATGTAGCTGAAT+GGG | 0.407553 | 8.3:+19043783 | MS.gene72986:CDS |
| AATTTGGTAGGTTTCAGCTT+TGG | 0.444763 | 8.3:+19044404 | MS.gene72986:intron |
| TAACATAACAAAGGAGAGTA+AGG | 0.457931 | 8.3:+19044727 | MS.gene72986:CDS |
| TCAATCCAAATGTAGCTGAA+TGG | 0.461891 | 8.3:+19043782 | MS.gene72986:CDS |
| TTGGAGGGTAATGTTTCTAA+AGG | 0.464546 | 8.3:+19044423 | MS.gene72986:CDS |
| AAGAATTGCAAAGAAGGAAA+AGG | 0.466398 | 8.3:+19044231 | MS.gene72986:CDS |
| GAAATTGCAGAGAAAAGAAC+AGG | 0.476993 | 8.3:+19044539 | MS.gene72986:CDS |
| CAGTTGTAGTAACAACCAAA+TGG | 0.480586 | 8.3:+19044822 | MS.gene72986:CDS |
| TTGGTAGGTTTCAGCTTTGG+AGG | 0.481735 | 8.3:+19044407 | MS.gene72986:intron |
| GAAATTAAGAATTGCAAAGA+AGG | 0.485109 | 8.3:+19044225 | MS.gene72986:CDS |
| AGGTGAAGTTATTTCAGAGT+TGG | 0.494651 | 8.3:+19044443 | MS.gene72986:CDS |
| AGTTGTAGTAACAACCAAAT+GGG | 0.521225 | 8.3:+19044823 | MS.gene72986:CDS |
| AACTGATATGAATTTGGTGA+TGG | 0.530668 | 8.3:-19043903 | None:intergenic |
| AAAATGTTGACAGAATCAAC+AGG | 0.539679 | 8.3:+19044061 | MS.gene72986:CDS |
| GATATTGAGAGAGTCAAAGA+TGG | 0.547900 | 8.3:+19044148 | MS.gene72986:CDS |
| ACATCGAGAGTAGAGAAAGA+AGG | 0.558063 | 8.3:+19044194 | MS.gene72986:CDS |
| ACTGACTCAGAATGTTTCAA+AGG | 0.560272 | 8.3:-19044803 | None:intergenic |
| GATGAGAGTATTGATTACAT+TGG | 0.562495 | 8.3:+19044491 | MS.gene72986:CDS |
| TTGGATGAAATTTATGGTGA+GGG | 0.566235 | 8.3:+19044510 | MS.gene72986:CDS |
| AAAACTTGCAATGCAGTCAA+AGG | 0.573016 | 8.3:+19043696 | None:intergenic |
| TGGTAGGTTTCAGCTTTGGA+GGG | 0.580586 | 8.3:+19044408 | MS.gene72986:intron |
| ATTTGGATTGACATGAACAT+TGG | 0.587267 | 8.3:-19043770 | None:intergenic |
| GCTGAATGGGAATTAAGACC+AGG | 0.631294 | 8.3:+19043796 | MS.gene72986:CDS |
| ACTGATATGAATTTGGTGAT+GGG | 0.633798 | 8.3:-19043902 | None:intergenic |
| ATTGGATGAAATTTATGGTG+AGG | 0.644553 | 8.3:+19044509 | MS.gene72986:CDS |
| TGCAGTCAAAGGTTACAAAG+AGG | 0.668299 | 8.3:+19043707 | MS.gene72986:CDS |
| GAATGGGAATTAAGACCAGG+TGG | 0.675489 | 8.3:+19043799 | MS.gene72986:CDS |
| AAATAACAATAACATAACAA+AGG | 0.682250 | 8.3:+19044718 | MS.gene72986:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAAAATTAATGTGT+AGG | + | chr8.3:19044634-19044653 | MS.gene72986:intron | 10.0% |
| !! | AAATAACAATAACATAACAA+AGG | + | chr8.3:19044718-19044737 | MS.gene72986:CDS | 15.0% |
| !! | CTAAATAATTCAATGAAATT+TGG | + | chr8.3:19044388-19044407 | MS.gene72986:intron | 15.0% |
| !! | TGATTATTATTGGATTATTA+TGG | + | chr8.3:19044013-19044032 | MS.gene72986:intron | 15.0% |
| !! | TTAAAATATGCAAAGTATAA+AGG | - | chr8.3:19043969-19043988 | None:intergenic | 15.0% |
| !! | AAGTATAAAGGAAATTCTAA+AGG | - | chr8.3:19043957-19043976 | None:intergenic | 20.0% |
| !! | ATAAAATTAATGTGTAGGTT+AGG | + | chr8.3:19044639-19044658 | MS.gene72986:intron | 20.0% |
| !! | ATAATTCAATGAAATTTGGT+AGG | + | chr8.3:19044392-19044411 | MS.gene72986:intron | 20.0% |
| !! | ATTACATTGGATGAAATTTA+TGG | + | chr8.3:19044504-19044523 | MS.gene72986:CDS | 20.0% |
| !! | TTTCAACATTGTAAAAATAG+TGG | + | chr8.3:19044365-19044384 | MS.gene72986:intron | 20.0% |
| !!! | TGATTGATGATGATTATTAT+TGG | + | chr8.3:19044003-19044022 | MS.gene72986:intron | 20.0% |
| ! | GAAATTAAGAATTGCAAAGA+AGG | + | chr8.3:19044225-19044244 | MS.gene72986:CDS | 25.0% |
| ! | TTAATCAAAAGTTTCCCATT+TGG | - | chr8.3:19044840-19044859 | None:intergenic | 25.0% |
| !! | TTGGATTATTATGGTTTTTG+TGG | + | chr8.3:19044022-19044041 | MS.gene72986:intron | 25.0% |
| AAAATGTTGACAGAATCAAC+AGG | + | chr8.3:19044061-19044080 | MS.gene72986:CDS | 30.0% | |
| AAGAATTGCAAAGAAGGAAA+AGG | + | chr8.3:19044231-19044250 | MS.gene72986:CDS | 30.0% | |
| AGTTGTAGTAACAACCAAAT+GGG | + | chr8.3:19044823-19044842 | MS.gene72986:CDS | 30.0% | |
| ATCAAAGTCAAAGTGAAGTA+TGG | + | chr8.3:19043874-19043893 | MS.gene72986:CDS | 30.0% | |
| ATTGGATGAAATTTATGGTG+AGG | + | chr8.3:19044509-19044528 | MS.gene72986:CDS | 30.0% | |
| ATTTGGATTGACATGAACAT+TGG | - | chr8.3:19043773-19043792 | None:intergenic | 30.0% | |
| CAGTTATGAAAACAAAGAGT+TGG | - | chr8.3:19044568-19044587 | None:intergenic | 30.0% | |
| CATGAGAACTGATATGAATT+TGG | - | chr8.3:19043912-19043931 | None:intergenic | 30.0% | |
| CTTAATTGAAGTAACCTTAG+AGG | + | chr8.3:19044264-19044283 | MS.gene72986:CDS | 30.0% | |
| TAACATAACAAAGGAGAGTA+AGG | + | chr8.3:19044727-19044746 | MS.gene72986:CDS | 30.0% | |
| TTGGATGAAATTTATGGTGA+GGG | + | chr8.3:19044510-19044529 | MS.gene72986:CDS | 30.0% | |
| ! | AACGATCAACATGTTTTCAA+GGG | + | chr8.3:19043732-19043751 | MS.gene72986:CDS | 30.0% |
| ! | AACTGATATGAATTTGGTGA+TGG | - | chr8.3:19043906-19043925 | None:intergenic | 30.0% |
| ! | ACTGATATGAATTTGGTGAT+GGG | - | chr8.3:19043905-19043924 | None:intergenic | 30.0% |
| ! | ATTATGGTTTTTGTGGTGTA+GGG | + | chr8.3:19044029-19044048 | MS.gene72986:intron | 30.0% |
| ! | CACATTATTGTGTACCTTTT+TGG | - | chr8.3:19044302-19044321 | None:intergenic | 30.0% |
| ! | TATTATGGTTTTTGTGGTGT+AGG | + | chr8.3:19044028-19044047 | MS.gene72986:intron | 30.0% |
| !! | CTCTAAGGTTACTTCAATTA+AGG | - | chr8.3:19044266-19044285 | None:intergenic | 30.0% |
| !! | GATGAGAGTATTGATTACAT+TGG | + | chr8.3:19044491-19044510 | MS.gene72986:CDS | 30.0% |
| !!! | CAACTCTTTGTTTTCATAAC+TGG | + | chr8.3:19044566-19044585 | MS.gene72986:intron | 30.0% |
| AATTCCCATTCAGCTACATT+TGG | - | chr8.3:19043790-19043809 | None:intergenic | 35.0% | |
| AATTTGGTAGGTTTCAGCTT+TGG | + | chr8.3:19044404-19044423 | MS.gene72986:intron | 35.0% | |
| CAATCCAAATGTAGCTGAAT+GGG | + | chr8.3:19043783-19043802 | MS.gene72986:CDS | 35.0% | |
| CAGTTGTAGTAACAACCAAA+TGG | + | chr8.3:19044822-19044841 | MS.gene72986:CDS | 35.0% | |
| GAAATTGCAGAGAAAAGAAC+AGG | + | chr8.3:19044539-19044558 | MS.gene72986:CDS | 35.0% | |
| GAGTAGAGAAAGAAGGATAT+TGG | + | chr8.3:19044201-19044220 | MS.gene72986:CDS | 35.0% | |
| GATATTGAGAGAGTCAAAGA+TGG | + | chr8.3:19044148-19044167 | MS.gene72986:CDS | 35.0% | |
| TCAATCCAAATGTAGCTGAA+TGG | + | chr8.3:19043782-19043801 | MS.gene72986:CDS | 35.0% | |
| ! | ACTGACTCAGAATGTTTCAA+AGG | - | chr8.3:19044806-19044825 | None:intergenic | 35.0% |
| ! | AGGTGAAGTTATTTCAGAGT+TGG | + | chr8.3:19044443-19044462 | MS.gene72986:CDS | 35.0% |
| ! | ATCAGTTCTCATGCAAGTTT+TGG | + | chr8.3:19043919-19043938 | MS.gene72986:CDS | 35.0% |
| ! | CAACGATCAACATGTTTTCA+AGG | + | chr8.3:19043731-19043750 | MS.gene72986:CDS | 35.0% |
| ! | CTTCACTTTGACTTTGATGA+TGG | - | chr8.3:19043872-19043891 | None:intergenic | 35.0% |
| ! | TTATGGTTTTTGTGGTGTAG+GGG | + | chr8.3:19044030-19044049 | MS.gene72986:intron | 35.0% |
| ! | TTGGAGGGTAATGTTTCTAA+AGG | + | chr8.3:19044423-19044442 | MS.gene72986:CDS | 35.0% |
| ACATCGAGAGTAGAGAAAGA+AGG | + | chr8.3:19044194-19044213 | MS.gene72986:CDS | 40.0% | |
| GGTTGACAAACTTGCCAAAA+AGG | + | chr8.3:19044285-19044304 | MS.gene72986:CDS | 40.0% | |
| TGCAGTCAAAGGTTACAAAG+AGG | + | chr8.3:19043707-19043726 | MS.gene72986:CDS | 40.0% | |
| ! | TTTTGTACTAGCATTCCACC+TGG | - | chr8.3:19043817-19043836 | None:intergenic | 40.0% |
| GAATGGGAATTAAGACCAGG+TGG | + | chr8.3:19043799-19043818 | MS.gene72986:CDS | 45.0% | |
| GCTGAATGGGAATTAAGACC+AGG | + | chr8.3:19043796-19043815 | MS.gene72986:CDS | 45.0% | |
| GGCAAGTTTGTCAACCTCTA+AGG | - | chr8.3:19044281-19044300 | None:intergenic | 45.0% | |
| ! | TTGGTAGGTTTCAGCTTTGG+AGG | + | chr8.3:19044407-19044426 | MS.gene72986:intron | 45.0% |
| !! | TGGTAGGTTTCAGCTTTGGA+GGG | + | chr8.3:19044408-19044427 | MS.gene72986:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19043706 | 19044859 | 19043706 | ID=MS.gene72986 |
| chr8.3 | mRNA | 19043706 | 19044859 | 19043706 | ID=MS.gene72986.t1;Parent=MS.gene72986 |
| chr8.3 | exon | 19043706 | 19043940 | 19043706 | ID=MS.gene72986.t1.exon1;Parent=MS.gene72986.t1 |
| chr8.3 | CDS | 19043706 | 19043940 | 19043706 | ID=cds.MS.gene72986.t1;Parent=MS.gene72986.t1 |
| chr8.3 | exon | 19044050 | 19044306 | 19044050 | ID=MS.gene72986.t1.exon2;Parent=MS.gene72986.t1 |
| chr8.3 | CDS | 19044050 | 19044306 | 19044050 | ID=cds.MS.gene72986.t1;Parent=MS.gene72986.t1 |
| chr8.3 | exon | 19044414 | 19044560 | 19044414 | ID=MS.gene72986.t1.exon3;Parent=MS.gene72986.t1 |
| chr8.3 | CDS | 19044414 | 19044560 | 19044414 | ID=cds.MS.gene72986.t1;Parent=MS.gene72986.t1 |
| chr8.3 | exon | 19044656 | 19044859 | 19044656 | ID=MS.gene72986.t1.exon4;Parent=MS.gene72986.t1 |
| chr8.3 | CDS | 19044656 | 19044859 | 19044656 | ID=cds.MS.gene72986.t1;Parent=MS.gene72986.t1 |
| Gene Sequence |
| Protein sequence |
